AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0022640


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0022640 MtrunA17_Chr1g0175821 46.316 190 92 1 6 185 26 215 4.46e-48 155
Msa0022640 MtrunA17_Chr1g0175811 40.724 221 81 6 1 186 1 206 1.33e-36 126
Msa0022640 MtrunA17_Chr1g0175791 44.571 175 64 4 8 155 67 235 1.34e-29 109
Msa0022640 MtrunA17_Chr5g0420601 33.333 201 96 5 21 186 85 282 2.11e-18 80.5
Msa0022640 MtrunA17_Chr1g0175781 30.769 208 95 5 21 186 90 290 4.85e-15 71.2
Msa0022640 MtrunA17_Chr1g0175751 28.846 208 99 5 21 186 119 319 9.46e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAACTTGGTCAAGGTTTA+AGG 0.142695 1_1:+42538426 None:intergenic
CTTGTAAACAATTCCTTCTT+TGG 0.221022 1_1:+42538568 None:intergenic
CTTCGAAAAGAGTGGATTAA+TGG 0.276341 1_1:-42538645 Msa0022640:CDS
CTTGGTCAAGGTTTAAGGTT+AGG 0.388940 1_1:+42538431 None:intergenic
ATGGCTACTCTACATCAATC+TGG 0.390287 1_1:-42538913 Msa0022640:CDS
GCAAGCAACACCCGTTCAAT+TGG 0.411385 1_1:-42538816 Msa0022640:CDS
TGCTCGTTGTATGGAGCAAA+AGG 0.418843 1_1:-42538786 Msa0022640:CDS
GGAAAATTGCACACCAAAGA+AGG 0.418990 1_1:-42538581 Msa0022640:CDS
GGTCTCGATTGCTCGTTGTA+TGG 0.434380 1_1:-42538795 Msa0022640:CDS
CAATAAAATTGAAGAATTGA+AGG 0.437348 1_1:-42538519 Msa0022640:CDS
AGAAGGAATTGTTTACAAGT+TGG 0.457133 1_1:-42538564 Msa0022640:CDS
CTGAATCTGATAATAACATA+GGG 0.473665 1_1:-42538697 Msa0022640:CDS
AGGAATTGTTTACAAGTTGG+AGG 0.487776 1_1:-42538561 Msa0022640:CDS
AAAGGATCATGAGGCATATG+AGG 0.490646 1_1:-42538870 Msa0022640:CDS
ATTGTAGTTCAGCACAATGT+TGG 0.502018 1_1:-42538491 Msa0022640:CDS
TCTGAATCTGATAATAACAT+AGG 0.504914 1_1:-42538698 Msa0022640:CDS
GAGTGGATTAATGGAAAGAA+CGG 0.524492 1_1:-42538636 Msa0022640:CDS
GCACAATGTTGGCTTTAATG+AGG 0.525326 1_1:-42538480 Msa0022640:CDS
TCATGAGGCATATGAGGAGT+GGG 0.527499 1_1:-42538864 Msa0022640:CDS
GCAATCGAGACCAATTGAAC+GGG 0.527802 1_1:+42538806 None:intergenic
GTAGAGTAGCCATTCTCAGC+TGG 0.533250 1_1:+42538923 None:intergenic
AAGACTACACCAGCTGAGAA+TGG 0.552919 1_1:-42538932 Msa0022640:CDS
TGGCTACTCTACATCAATCT+GGG 0.557094 1_1:-42538912 Msa0022640:CDS
GAGTTAATCTTCGAAAAGAG+TGG 0.570774 1_1:-42538653 Msa0022640:CDS
ATCATGAGGCATATGAGGAG+TGG 0.581486 1_1:-42538865 Msa0022640:CDS
AGAATGGATCCCAAGAGATG+TGG 0.586826 1_1:-42538977 Msa0022640:CDS
CGGGTGTTGCTTGCAAAACA+CGG 0.601763 1_1:+42538825 None:intergenic
TATCACAGAAAAGGATCATG+AGG 0.608121 1_1:-42538879 Msa0022640:CDS
AGCAATCGAGACCAATTGAA+CGG 0.624783 1_1:+42538805 None:intergenic
TGAATCTGATAATAACATAG+GGG 0.669556 1_1:-42538696 Msa0022640:CDS
CATGAGGCATATGAGGAGTG+GGG 0.686751 1_1:-42538863 Msa0022640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAAAATTGAAGAATTGA+AGG - chr1_1:42538891-42538910 Msa0022640:CDS 20.0%
!! TGACAATTTTATCACAGAAA+AGG - chr1_1:42538522-42538541 Msa0022640:CDS 25.0%
! TCTGAATCTGATAATAACAT+AGG - chr1_1:42538712-42538731 Msa0022640:CDS 25.0%
! CTGAATCTGATAATAACATA+GGG - chr1_1:42538713-42538732 Msa0022640:CDS 25.0%
! TGAATCTGATAATAACATAG+GGG - chr1_1:42538714-42538733 Msa0022640:CDS 25.0%
!! AATGAAAACTCCAAGATTTT+AGG - chr1_1:42538808-42538827 Msa0022640:CDS 25.0%
! GTGCAATTTTCCTAAAATCT+TGG + chr1_1:42538821-42538840 None:intergenic 30.0%
CTTGTAAACAATTCCTTCTT+TGG + chr1_1:42538845-42538864 None:intergenic 30.0%
AGAAGGAATTGTTTACAAGT+TGG - chr1_1:42538846-42538865 Msa0022640:CDS 30.0%
TATCACAGAAAAGGATCATG+AGG - chr1_1:42538531-42538550 Msa0022640:CDS 35.0%
TGATTTGAAAAAGAGCTGCA+GGG - chr1_1:42538651-42538670 Msa0022640:CDS 35.0%
GAGTTAATCTTCGAAAAGAG+TGG - chr1_1:42538757-42538776 Msa0022640:CDS 35.0%
!! CTTCGAAAAGAGTGGATTAA+TGG - chr1_1:42538765-42538784 Msa0022640:CDS 35.0%
!! GAGTGGATTAATGGAAAGAA+CGG - chr1_1:42538774-42538793 Msa0022640:CDS 35.0%
! AGGAATTGTTTACAAGTTGG+AGG - chr1_1:42538849-42538868 Msa0022640:CDS 35.0%
ATTGTAGTTCAGCACAATGT+TGG - chr1_1:42538919-42538938 Msa0022640:CDS 35.0%
! CTACGTTTTCCACATCTCTT+GGG + chr1_1:42538445-42538464 None:intergenic 40.0%
ATGGCTACTCTACATCAATC+TGG - chr1_1:42538497-42538516 Msa0022640:CDS 40.0%
TGGCTACTCTACATCAATCT+GGG - chr1_1:42538498-42538517 Msa0022640:CDS 40.0%
AAAGGATCATGAGGCATATG+AGG - chr1_1:42538540-42538559 Msa0022640:CDS 40.0%
AGCAATCGAGACCAATTGAA+CGG + chr1_1:42538608-42538627 None:intergenic 40.0%
GTGATTTGAAAAAGAGCTGC+AGG - chr1_1:42538650-42538669 Msa0022640:CDS 40.0%
GGAAAATTGCACACCAAAGA+AGG - chr1_1:42538829-42538848 Msa0022640:CDS 40.0%
!! GCACAATGTTGGCTTTAATG+AGG - chr1_1:42538930-42538949 Msa0022640:CDS 40.0%
! GCTACGTTTTCCACATCTCT+TGG + chr1_1:42538446-42538465 None:intergenic 45.0%
AAGACTACACCAGCTGAGAA+TGG - chr1_1:42538478-42538497 Msa0022640:CDS 45.0%
ATCATGAGGCATATGAGGAG+TGG - chr1_1:42538545-42538564 Msa0022640:CDS 45.0%
TCATGAGGCATATGAGGAGT+GGG - chr1_1:42538546-42538565 Msa0022640:CDS 45.0%
GCAATCGAGACCAATTGAAC+GGG + chr1_1:42538607-42538626 None:intergenic 45.0%
TGCTCGTTGTATGGAGCAAA+AGG - chr1_1:42538624-42538643 Msa0022640:CDS 45.0%
GTAGAGTAGCCATTCTCAGC+TGG + chr1_1:42538490-42538509 None:intergenic 50.0%
CATGAGGCATATGAGGAGTG+GGG - chr1_1:42538547-42538566 Msa0022640:CDS 50.0%
!! CGGGTGTTGCTTGCAAAACA+CGG + chr1_1:42538588-42538607 None:intergenic 50.0%
GCAAGCAACACCCGTTCAAT+TGG - chr1_1:42538594-42538613 Msa0022640:CDS 50.0%
GGTCTCGATTGCTCGTTGTA+TGG - chr1_1:42538615-42538634 Msa0022640:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 42538436 42538996 42538436 ID=Msa0022640;Name=Msa0022640
chr1_1 mRNA 42538436 42538996 42538436 ID=Msa0022640-mRNA-1;Parent=Msa0022640;Name=Msa0022640-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|186
chr1_1 exon 42538436 42538996 42538436 ID=Msa0022640-mRNA-1:exon:12457;Parent=Msa0022640-mRNA-1
chr1_1 CDS 42538436 42538996 42538436 ID=Msa0022640-mRNA-1:cds;Parent=Msa0022640-mRNA-1
Gene Sequence

>Msa0022640

ATGGATCCCAAGAGATGTGGAAAACGTAGCAAAAGAAAGAGAAAGACTACACCAGCTGAGAATGGCTACTCTACATCAATCTGGGATGACAATTTTATCACAGAAAAGGATCATGAGGCATATGAGGAGTGGGGATTAGAAAAGTGCTTCCGTGTTTTGCAAGCAACACCCGTTCAATTGGTCTCGATTGCTCGTTGTATGGAGCAAAAGGTTTGTGATTTGAAAAAGAGCTGCAGGGCTCACATCGAAGAGAATACTAAATTGAAAAAGAAGTTGTCTGAATCTGATAATAACATAGGGGAATTAAAGAGAAAGTTCGAAGAGTTAATCTTCGAAAAGAGTGGATTAATGGAAAGAACGGAAGACTTGAAGAATGAAAACTCCAAGATTTTAGGAAAATTGCACACCAAAGAAGGAATTGTTTACAAGTTGGAGGCTGAAATTAAAGAGCTGAACAATAAAATTGAAGAATTGAAGGATGAAATTGTAGTTCAGCACAATGTTGGCTTTAATGAGGCTGTCATACAATTTTTAACTTCAATACCTAACCTTAAACCTTGA

Protein sequence

>Msa0022640

MDPKRCGKRSKRKRKTTPAENGYSTSIWDDNFITEKDHEAYEEWGLEKCFRVLQATPVQLVSIARCMEQKVCDLKKSCRAHIEENTKLKKKLSESDNNIGELKRKFEELIFEKSGLMERTEDLKNENSKILGKLHTKEGIVYKLEAEIKELNNKIEELKDEIVVQHNVGFNEAVIQFLTSIPNLKP*