AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1171400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171400 MtrunA17_Chr7g0266441 95.515 379 15 1 1 379 1 377 0.0 725
Msa1171400 MtrunA17_Chr1g0178491 64.042 381 125 6 2 377 1 374 5.40e-173 487
Msa1171400 MtrunA17_Chr7g0266431 87.234 188 23 1 189 375 2 189 8.98e-120 344
Msa1171400 MtrunA17_Chr7g0266421 86.667 90 12 0 14 103 10 99 1.78e-48 160
Msa1171400 MtrunA17_Chr3g0143611 25.826 333 185 14 34 329 5 312 7.78e-14 72.0
Msa1171400 MtrunA17_Chr2g0294691 29.814 161 101 5 214 371 43 194 7.88e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171400 AT1G57790.1 44.789 355 182 6 24 374 3 347 3.45e-101 303
Msa1171400 AT3G56470.1 41.880 351 179 7 32 376 27 358 3.96e-82 255
Msa1171400 AT4G00893.1 39.489 352 198 8 33 378 44 386 2.06e-79 249
Msa1171400 AT4G10660.1 41.000 300 161 6 62 354 3 293 9.08e-68 217
Msa1171400 AT4G12370.1 38.835 309 170 8 81 379 1 300 1.48e-67 216
Msa1171400 AT1G49360.5 34.527 391 213 15 8 372 61 434 6.97e-52 179
Msa1171400 AT1G49360.1 34.527 391 213 15 8 372 84 457 1.31e-51 179
Msa1171400 AT1G49360.3 35.813 363 192 14 37 373 8 355 1.76e-51 176
Msa1171400 AT1G49360.2 35.813 363 192 14 37 373 8 355 1.76e-51 176
Msa1171400 AT1G49360.4 35.813 363 192 14 37 373 8 355 1.76e-51 176
Msa1171400 AT3G18720.1 34.085 355 189 11 36 374 51 376 2.35e-50 173
Msa1171400 AT4G10695.2 43.216 199 97 3 46 238 1 189 3.88e-48 163
Msa1171400 AT4G10695.1 43.216 199 97 3 46 238 1 189 3.88e-48 163
Msa1171400 AT4G12382.1 45.455 132 66 2 33 164 9 134 1.37e-35 127
Msa1171400 AT4G12382.2 45.455 132 66 2 33 164 9 134 1.37e-35 127
Msa1171400 AT2G44735.1 40.260 154 78 5 139 288 109 252 5.73e-23 99.4
Msa1171400 AT4G10700.1 41.270 126 66 3 258 376 119 243 1.19e-20 90.5
Msa1171400 AT4G10700.1 47.312 93 43 2 62 154 3 89 4.02e-20 89.0

Find 103 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATCTAACTTGAAAATTAT+TGG 0.158009 8_1:+1018587 None:intergenic
GAACCACCAAAAGACTTTAT+TGG 0.163822 8_1:-1018326 Msa1171400:CDS
CAGAAGTTCTCAAAAGTATT+TGG 0.184683 8_1:+1018353 None:intergenic
TTCCCTAAAGTCCGTTTCTA+TGG 0.238841 8_1:-1018449 Msa1171400:CDS
AGCTAAAATATTTATCTCTT+TGG 0.254305 8_1:-1017031 Msa1171400:three_prime_UTR
CCTATGTACAACCTTCAAAT+AGG 0.266334 8_1:+1017092 None:intergenic
TTTCCATAGAAACGGACTTT+AGG 0.272121 8_1:+1018446 None:intergenic
GATCTAACTTGAAAATTATT+GGG 0.325599 8_1:+1018588 None:intergenic
CTCTCTTTAATCCCTTTACT+AGG 0.326943 8_1:-1019021 Msa1171400:CDS
CAAGTTAGATCTGACACTAA+TGG 0.329403 8_1:-1018576 Msa1171400:CDS
ATCCAGCCAATAAAGTCTTT+TGG 0.339525 8_1:+1018320 None:intergenic
AGAACTCTCTGCTAAGCATT+TGG 0.351257 8_1:+1018694 None:intergenic
TTGACCCACTTGAACGTACT+TGG 0.354769 8_1:-1018739 Msa1171400:CDS
TTCCATAGAAACGGACTTTA+GGG 0.361226 8_1:+1018447 None:intergenic
AACATTCTTCGCATGATGTC+GGG 0.375799 8_1:-1019951 Msa1171400:exon
TCTTGTCTCGGTTGGATGTT+GGG 0.383783 8_1:-1019300 Msa1171400:CDS
CCATGTTTGAGTGAAGCATT+TGG 0.387770 8_1:-1017203 Msa1171400:three_prime_UTR
GCTATAAGCACGTGTTATCC+TGG 0.393896 8_1:-1018881 Msa1171400:CDS
ATGTTCCACAAAAGCATTGC+AGG 0.397891 8_1:+1016968 None:intergenic
GAACTCTCTGCTAAGCATTT+GGG 0.407425 8_1:+1018695 None:intergenic
ATGGTTGGTTATTGCTAAAC+CGG 0.409750 8_1:-1019081 Msa1171400:CDS
GGAATTGTTCTTGTCTCGGT+TGG 0.421431 8_1:-1019308 Msa1171400:CDS
GATTAGCAAAACCTATTTGA+AGG 0.423915 8_1:-1017103 Msa1171400:three_prime_UTR
TTCCCTTGAGTTGCCAGAGT+TGG 0.426018 8_1:-1019131 Msa1171400:CDS
CTGCTTGTCGTGCAGGAACT+AGG 0.426509 8_1:-1017252 Msa1171400:intron
AACAATTCCAAGACTTCAGC+AGG 0.429634 8_1:+1019322 None:intergenic
TTCTTGTCTCGGTTGGATGT+TGG 0.436225 8_1:-1019301 Msa1171400:CDS
TCTTGGAATTGTTCTTGTCT+CGG 0.438217 8_1:-1019312 Msa1171400:CDS
TCTTGTGCTCCAACATCAAC+CGG 0.442241 8_1:-1018947 Msa1171400:CDS
TATTGCTAAACCGGCAGGAC+TGG 0.447316 8_1:-1019072 Msa1171400:CDS
ACAATTTGGAGCTGCAAACA+TGG 0.451046 8_1:-1019354 Msa1171400:CDS
TTCGCATGATGTCGGGGAAA+AGG 0.455236 8_1:-1019944 Msa1171400:exon
CTCTGGCAACTCAAGGGAAT+AGG 0.460842 8_1:+1019135 None:intergenic
CGATCTCCCTGCTGAAGTCT+TGG 0.469551 8_1:-1019329 Msa1171400:CDS
AAAGAGGTGAGAACACTAGA+TGG 0.470190 8_1:-1018548 Msa1171400:CDS
GAATAGGTCTTACGCTGCAC+AGG 0.470767 8_1:+1019151 None:intergenic
TCTCTTTAATCCCTTTACTA+GGG 0.471216 8_1:-1019020 Msa1171400:CDS
ATGTGGTCCTTTGATGTCAC+AGG 0.471463 8_1:-1017142 Msa1171400:three_prime_UTR
TGATATGTCCTGTCAAACTT+TGG 0.477303 8_1:+1018983 None:intergenic
GAAAATTAATATAACACATC+CGG 0.478730 8_1:+1018928 None:intergenic
AATACATAAGCCAAGGTGAT+TGG 0.482476 8_1:+1019209 None:intergenic
CTTGAGTTGCCAGAGTTGGA+TGG 0.483726 8_1:-1019127 Msa1171400:CDS
CCTGTCACGACACTGCTCAC+TGG 0.484710 8_1:+1018382 None:intergenic
GTGATCAGATCCCTAGTAAA+GGG 0.491655 8_1:+1019010 None:intergenic
CTGTCACGACACTGCTCACT+GGG 0.495007 8_1:+1018383 None:intergenic
CACCAAAAGACTTTATTGGC+TGG 0.495682 8_1:-1018322 Msa1171400:CDS
CTGAGCGACAGAGGTTGGCT+AGG 0.495793 8_1:-1018767 Msa1171400:CDS
TGAGCGACAGAGGTTGGCTA+GGG 0.496901 8_1:-1018766 Msa1171400:CDS
CCAACCTCCGCCAGTCCTGC+CGG 0.497826 8_1:+1019062 None:intergenic
CCAGTGAGCAGTGTCGTGAC+AGG 0.497864 8_1:-1018382 Msa1171400:CDS
CGTGTTTGCTATACAAAAGA+TGG 0.502296 8_1:-1019100 Msa1171400:CDS
CTATAAGCACGTGTTATCCT+GGG 0.505414 8_1:-1018880 Msa1171400:CDS
CAGGACTGGCGGAGGTTGGA+CGG 0.511557 8_1:-1019058 Msa1171400:CDS
CTTCAGCTGTTTGCAAGAGA+TGG 0.513153 8_1:-1019264 Msa1171400:CDS
CCTATTTGAAGGTTGTACAT+AGG 0.514646 8_1:-1017092 Msa1171400:three_prime_UTR
CCGGCAGGACTGGCGGAGGT+TGG 0.523905 8_1:-1019062 Msa1171400:CDS
ATTGTCTGAGCGACAGAGGT+TGG 0.524197 8_1:-1018772 Msa1171400:CDS
CAAGACTTCAGCAGGGAGAT+CGG 0.525981 8_1:+1019330 None:intergenic
CATTCTAGGACTTACGTCAC+TGG 0.528125 8_1:-1018494 Msa1171400:CDS
TTGGTTATTGCTAAACCGGC+AGG 0.529301 8_1:-1019077 Msa1171400:CDS
CGTGATCAGATCCCTAGTAA+AGG 0.536285 8_1:+1019009 None:intergenic
TCACGCTGCCAAAGTTTGAC+AGG 0.539518 8_1:-1018991 Msa1171400:CDS
CCAAATGCTTCACTCAAACA+TGG 0.541352 8_1:+1017203 None:intergenic
GTCACTGGAATAATGAGAAA+CGG 0.542182 8_1:-1018479 Msa1171400:CDS
TAGATCTGACACTAATGGAA+TGG 0.542919 8_1:-1018571 Msa1171400:CDS
TCTGCTAAGCATTTGGGCGG+AGG 0.544418 8_1:+1018701 None:intergenic
CAACATTCTTCGCATGATGT+CGG 0.545714 8_1:-1019952 Msa1171400:exon
TTTGCTATACAAAAGATGGT+TGG 0.546454 8_1:-1019096 Msa1171400:CDS
CATCCAACTCTGGCAACTCA+AGG 0.553068 8_1:+1019128 None:intergenic
ATAACACATCCGGTTGATGT+TGG 0.553987 8_1:+1018938 None:intergenic
TTGAAATATTAAGAAATCAA+CGG 0.556920 8_1:+1016876 None:intergenic
TTTGGGAAATACATAAGCCA+AGG 0.561817 8_1:+1019202 None:intergenic
CATAGGAAGTTAATATGTGA+AGG 0.564276 8_1:-1017075 Msa1171400:three_prime_UTR
GACACTAATGGAATGGAAAG+AGG 0.564517 8_1:-1018564 Msa1171400:CDS
TGATGTTGGAGCACAAGAGA+AGG 0.568366 8_1:+1018952 None:intergenic
TGCTAAACCGGCAGGACTGG+CGG 0.569550 8_1:-1019069 Msa1171400:CDS
CAATAGAAGAGCTGCTGCTG+AGG 0.574535 8_1:-1019386 Msa1171400:CDS
ACACGACATCCATCCAACTC+TGG 0.576746 8_1:+1019118 None:intergenic
CTTGTCTCGGTTGGATGTTG+GGG 0.581584 8_1:-1019299 Msa1171400:CDS
CTCTAAACCTGTGACATCAA+AGG 0.581595 8_1:+1017135 None:intergenic
AGGAATGAATATCTGTGAGT+AGG 0.583262 8_1:-1017055 Msa1171400:three_prime_UTR
GTGATTCTTCATGCAAAATG+TGG 0.585218 8_1:-1017159 Msa1171400:three_prime_UTR
ATCCAACTCTGGCAACTCAA+GGG 0.590798 8_1:+1019129 None:intergenic
TAAACCGGCAGGACTGGCGG+AGG 0.593956 8_1:-1019066 Msa1171400:CDS
ACAGTCCAAGTACGTTCAAG+TGG 0.603382 8_1:+1018734 None:intergenic
CAATTTGGAGCTGCAAACAT+GGG 0.606485 8_1:-1019353 Msa1171400:CDS
GCTCACTGGGATGATATCTA+CGG 0.610400 8_1:+1018396 None:intergenic
GTCGACTACACGTAAAGAAG+TGG 0.612484 8_1:+1019231 None:intergenic
ACATTCTTCGCATGATGTCG+GGG 0.620719 8_1:-1019950 Msa1171400:exon
CAGTGAGCAGTGTCGTGACA+GGG 0.621223 8_1:-1018381 Msa1171400:CDS
AATAGGTCTTACGCTGCACA+GGG 0.638547 8_1:+1019152 None:intergenic
CTCTCTGCTAAGCATTTGGG+CGG 0.648219 8_1:+1018698 None:intergenic
TTTGTAGTTTATATATGTTG+TGG 0.650518 8_1:-1018617 Msa1171400:CDS
AGTTTATATATGTTGTGGTG+AGG 0.651124 8_1:-1018612 Msa1171400:CDS
AGAATCACAAAGAACTCGAG+AGG 0.661545 8_1:+1017174 None:intergenic
GAGCGACAGAGGTTGGCTAG+GGG 0.671202 8_1:-1018765 Msa1171400:CDS
CAGTCCAAGTACGTTCAAGT+GGG 0.682692 8_1:+1018735 None:intergenic
GACCACTGTTAATTATGACG+CGG 0.704466 8_1:-1018846 Msa1171400:CDS
TATAAGCACGTGTTATCCTG+GGG 0.707016 8_1:-1018879 Msa1171400:CDS
ACAATTCCAAGACTTCAGCA+GGG 0.713188 8_1:+1019323 None:intergenic
GTGTAGTCGACCAATCACCT+TGG 0.718766 8_1:-1019219 Msa1171400:CDS
TACCGCGTCATAATTAACAG+TGG 0.732690 8_1:+1018844 None:intergenic
AAATTTATGATAGAGCACAA+AGG 0.741593 8_1:-1018647 Msa1171400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATCTTATTTTCAAATA+AGG - chr8_1:1019442-1019461 Msa1171400:intron 10.0%
!!! ATATTTCAATTATATTTTTG+TGG - chr8_1:1019970-1019989 Msa1171400:five_prime_UTR 10.0%
!! CAATAATAATAATATGTATG+GGG + chr8_1:1016965-1016984 None:intergenic 15.0%
!! GCAATAATAATAATATGTAT+GGG + chr8_1:1016966-1016985 None:intergenic 15.0%
!! AGCAATAATAATAATATGTA+TGG + chr8_1:1016967-1016986 None:intergenic 15.0%
!! TACATATTATTATTATTGCT+AGG - chr8_1:1016967-1016986 Msa1171400:three_prime_UTR 15.0%
!! ACATATTATTATTATTGCTA+GGG - chr8_1:1016968-1016987 Msa1171400:three_prime_UTR 15.0%
!!! AATTTTGTTCAACAATTAAT+TGG - chr8_1:1017012-1017031 Msa1171400:three_prime_UTR 15.0%
!! TTTAGATTGATAAACATATT+TGG - chr8_1:1017290-1017309 Msa1171400:intron 15.0%
!! GATGTAAAAAAAAAAATTAG+CGG - chr8_1:1018532-1018551 Msa1171400:CDS 15.0%
!! TTGAAATATTAAGAAATCAA+CGG + chr8_1:1019959-1019978 None:intergenic 15.0%
!!! TCAATTTTTTGGCTTAATAA+TGG - chr8_1:1017046-1017065 Msa1171400:three_prime_UTR 20.0%
!! TATGATTTCTATATCCAATT+CGG - chr8_1:1017105-1017124 Msa1171400:three_prime_UTR 20.0%
!! GTTTCATCTATATTTCATAT+TGG - chr8_1:1017141-1017160 Msa1171400:three_prime_UTR 20.0%
!! GAAAATTAATATAACACATC+CGG + chr8_1:1017907-1017926 None:intergenic 20.0%
!! TTTGTAGTTTATATATGTTG+TGG - chr8_1:1018215-1018234 Msa1171400:intron 20.0%
!! GATCTAACTTGAAAATTATT+GGG + chr8_1:1018247-1018266 None:intergenic 20.0%
!! AGATCTAACTTGAAAATTAT+TGG + chr8_1:1018248-1018267 None:intergenic 20.0%
!! AGACTACATATTATAACTAT+TGG - chr8_1:1018616-1018635 Msa1171400:CDS 20.0%
!!! ATTTTTCATTTCCAAAACTA+AGG + chr8_1:1018959-1018978 None:intergenic 20.0%
!!! TTAGTTTTGGAAATGAAAAA+TGG - chr8_1:1018958-1018977 Msa1171400:CDS 20.0%
!!! TTATTTTCAAATAAGGACTT+GGG - chr8_1:1019449-1019468 Msa1171400:intron 20.0%
!! AGCTAAAATATTTATCTCTT+TGG - chr8_1:1019801-1019820 Msa1171400:intron 20.0%
! ATAATAATATGTATGGGGTT+AGG + chr8_1:1016960-1016979 None:intergenic 25.0%
!!! TTGCACTAACTTCAATTTTT+TGG - chr8_1:1017035-1017054 Msa1171400:three_prime_UTR 25.0%
! CATGAAAAATTTAACCGAAT+TGG + chr8_1:1017122-1017141 None:intergenic 25.0%
! TAGTTAATTCACAATAAAGC+TGG + chr8_1:1017178-1017197 None:intergenic 25.0%
!!! CATTTTTAAGTTGATGATTG+TGG - chr8_1:1017199-1017218 Msa1171400:three_prime_UTR 25.0%
! AGATTGATAAACATATTTGG+TGG - chr8_1:1017293-1017312 Msa1171400:intron 25.0%
! GATTGATAAACATATTTGGT+GGG - chr8_1:1017294-1017313 Msa1171400:intron 25.0%
! CTTATGTATTTCCCAAAAAA+GGG - chr8_1:1017636-1017655 Msa1171400:intron 25.0%
!!! CATAACAATTACCCTTTTTT+GGG + chr8_1:1017650-1017669 None:intergenic 25.0%
!!! TCATAACAATTACCCTTTTT+TGG + chr8_1:1017651-1017670 None:intergenic 25.0%
!! ATATTAATTTTCAGACGTGT+TGG - chr8_1:1017915-1017934 Msa1171400:intron 25.0%
!! ATAAGCTTGTCTTTTCTAAT+GGG - chr8_1:1018030-1018049 Msa1171400:intron 25.0%
! AAATTTATGATAGAGCACAA+AGG - chr8_1:1018185-1018204 Msa1171400:intron 25.0%
!!! TTGTGTAGAGCATTTATTTT+TGG - chr8_1:1018581-1018600 Msa1171400:CDS 25.0%
!! TATTGGATACATGCTTTATT+AGG - chr8_1:1018633-1018652 Msa1171400:CDS 25.0%
! TATTAGGCTGTAAACATTTA+TGG - chr8_1:1018649-1018668 Msa1171400:CDS 25.0%
! CAATAAGACTAGGAAAATTT+AGG + chr8_1:1018728-1018747 None:intergenic 25.0%
! TGAGTTATATGCAAATCTTA+AGG - chr8_1:1018795-1018814 Msa1171400:CDS 25.0%
!!! CACTAGTTTGTTGTAAAAAT+TGG - chr8_1:1019074-1019093 Msa1171400:CDS 25.0%
!! TTTAACTTCAATCGAGATTA+TGG + chr8_1:1019141-1019160 None:intergenic 25.0%
!! AGGGTATTCTATAAAATAAG+AGG + chr8_1:1019242-1019261 None:intergenic 25.0%
! GATAGTTTGGAAAATATAGT+CGG - chr8_1:1019299-1019318 Msa1171400:CDS 25.0%
!!! CTTATTTTCAAATAAGGACT+TGG - chr8_1:1019448-1019467 Msa1171400:intron 25.0%
! AGAAAAAGATATGATCAAAC+AGG + chr8_1:1019536-1019555 None:intergenic 25.0%
TTGTTGAACAAAATTGAGAG+TGG + chr8_1:1017007-1017026 None:intergenic 30.0%
AAAAAATGAAGTTAGCTAGC+AGG + chr8_1:1017361-1017380 None:intergenic 30.0%
TGAGGTAAAAAGCAACAATT+TGG - chr8_1:1017464-1017483 Msa1171400:intron 30.0%
GCTTATGTATTTCCCAAAAA+AGG - chr8_1:1017635-1017654 Msa1171400:intron 30.0%
! TTTGCTATACAAAAGATGGT+TGG - chr8_1:1017736-1017755 Msa1171400:intron 30.0%
TCTCTTTAATCCCTTTACTA+GGG - chr8_1:1017812-1017831 Msa1171400:intron 30.0%
! GATAAGCTTGTCTTTTCTAA+TGG - chr8_1:1018029-1018048 Msa1171400:intron 30.0%
AGTTTATATATGTTGTGGTG+AGG - chr8_1:1018220-1018239 Msa1171400:intron 30.0%
!!! GTTTTTTGTCATCTCATTCT+AGG - chr8_1:1018324-1018343 Msa1171400:CDS 30.0%
!! CAGAAGTTCTCAAAAGTATT+TGG + chr8_1:1018482-1018501 None:intergenic 30.0%
!! CAAATACTTTTGAGAACTTC+TGG - chr8_1:1018480-1018499 Msa1171400:CDS 30.0%
TTGTAGCTAACAATAAGACT+AGG + chr8_1:1018738-1018757 None:intergenic 30.0%
AAATCTTAAGGTTTCTCCAA+TGG - chr8_1:1018807-1018826 Msa1171400:CDS 30.0%
! ATGTTCATGATCCTTAGTTT+TGG - chr8_1:1018945-1018964 Msa1171400:CDS 30.0%
! ATTTTATAGAATACCCTTGC+AGG - chr8_1:1019245-1019264 Msa1171400:CDS 30.0%
! CTATATTTTCCAAACTATCG+AGG + chr8_1:1019298-1019317 None:intergenic 30.0%
ACTGGTATATGATTGCTAAA+TGG - chr8_1:1019351-1019370 Msa1171400:CDS 30.0%
! ATTTTGCCTGTTTGATCAAA+TGG - chr8_1:1019395-1019414 Msa1171400:CDS 30.0%
GATTAGCAAAACCTATTTGA+AGG - chr8_1:1019729-1019748 Msa1171400:intron 30.0%
CATAGGAAGTTAATATGTGA+AGG - chr8_1:1019757-1019776 Msa1171400:intron 30.0%
GGAGAAAAATCTTGAAATTC+AGG + chr8_1:1019846-1019865 None:intergenic 30.0%
ATATGTATGGGGTTAGGTTA+GGG + chr8_1:1016954-1016973 None:intergenic 35.0%
AATATGTATGGGGTTAGGTT+AGG + chr8_1:1016955-1016974 None:intergenic 35.0%
ATTCACAATAAAGCTGGTCA+TGG + chr8_1:1017172-1017191 None:intergenic 35.0%
GTAAACAGAACAGAAACAGT+GGG + chr8_1:1017255-1017274 None:intergenic 35.0%
AGTAAACAGAACAGAAACAG+TGG + chr8_1:1017256-1017275 None:intergenic 35.0%
! GATAAACATATTTGGTGGGA+TGG - chr8_1:1017298-1017317 Msa1171400:intron 35.0%
TCTTGGAATTGTTCTTGTCT+CGG - chr8_1:1017520-1017539 Msa1171400:intron 35.0%
AATACATAAGCCAAGGTGAT+TGG + chr8_1:1017626-1017645 None:intergenic 35.0%
TTTGGGAAATACATAAGCCA+AGG + chr8_1:1017633-1017652 None:intergenic 35.0%
!! CGTGTTTGCTATACAAAAGA+TGG - chr8_1:1017732-1017751 Msa1171400:intron 35.0%
! ATGGTTGGTTATTGCTAAAC+CGG - chr8_1:1017751-1017770 Msa1171400:intron 35.0%
CTCTCTTTAATCCCTTTACT+AGG - chr8_1:1017811-1017830 Msa1171400:intron 35.0%
TGATATGTCCTGTCAAACTT+TGG + chr8_1:1017852-1017871 None:intergenic 35.0%
CAAGTTAGATCTGACACTAA+TGG - chr8_1:1018256-1018275 Msa1171400:intron 35.0%
TAGATCTGACACTAATGGAA+TGG - chr8_1:1018261-1018280 Msa1171400:intron 35.0%
GTCACTGGAATAATGAGAAA+CGG - chr8_1:1018353-1018372 Msa1171400:CDS 35.0%
! TTCCATAGAAACGGACTTTA+GGG + chr8_1:1018388-1018407 None:intergenic 35.0%
! TTTCCATAGAAACGGACTTT+AGG + chr8_1:1018389-1018408 None:intergenic 35.0%
! TGCAACGTTTTCCATAGAAA+CGG + chr8_1:1018397-1018416 None:intergenic 35.0%
GAACCACCAAAAGACTTTAT+TGG - chr8_1:1018506-1018525 Msa1171400:CDS 35.0%
! ATCCAGCCAATAAAGTCTTT+TGG + chr8_1:1018515-1018534 None:intergenic 35.0%
GTAAACATTTATGGAGCTAC+TGG - chr8_1:1018658-1018677 Msa1171400:CDS 35.0%
GAATAAGATTGCATGCATCA+TGG + chr8_1:1018771-1018790 None:intergenic 35.0%
ACTAATGCACATGTAACCAT+TGG + chr8_1:1018826-1018845 None:intergenic 35.0%
! GACAGAGTACAATTTTGCTA+TGG - chr8_1:1018894-1018913 Msa1171400:CDS 35.0%
TGAAAAATGGTCAGAGTTAG+AGG - chr8_1:1018971-1018990 Msa1171400:CDS 35.0%
ACACACATGACTTTCTATGA+TGG + chr8_1:1019108-1019127 None:intergenic 35.0%
! ATTTTCCAAACTATCGAGGA+TGG + chr8_1:1019294-1019313 None:intergenic 35.0%
ATCCAACCATTTGATCAAAC+AGG + chr8_1:1019404-1019423 None:intergenic 35.0%
GTGATTCTTCATGCAAAATG+TGG - chr8_1:1019673-1019692 Msa1171400:intron 35.0%
CCTATGTACAACCTTCAAAT+AGG + chr8_1:1019743-1019762 None:intergenic 35.0%
CCTATTTGAAGGTTGTACAT+AGG - chr8_1:1019740-1019759 Msa1171400:intron 35.0%
AGGAATGAATATCTGTGAGT+AGG - chr8_1:1019777-1019796 Msa1171400:intron 35.0%
CAACATTCTTCGCATGATGT+CGG - chr8_1:1016880-1016899 Msa1171400:three_prime_UTR 40.0%
AACATTCTTCGCATGATGTC+GGG - chr8_1:1016881-1016900 Msa1171400:three_prime_UTR 40.0%
! ACAATTTGGAGCTGCAAACA+TGG - chr8_1:1017478-1017497 Msa1171400:intron 40.0%
! CAATTTGGAGCTGCAAACAT+GGG - chr8_1:1017479-1017498 Msa1171400:intron 40.0%
ACAATTCCAAGACTTCAGCA+GGG + chr8_1:1017512-1017531 None:intergenic 40.0%
AACAATTCCAAGACTTCAGC+AGG + chr8_1:1017513-1017532 None:intergenic 40.0%
GTGATCAGATCCCTAGTAAA+GGG + chr8_1:1017825-1017844 None:intergenic 40.0%
! ATAACACATCCGGTTGATGT+TGG + chr8_1:1017897-1017916 None:intergenic 40.0%
CTATAAGCACGTGTTATCCT+GGG - chr8_1:1017952-1017971 Msa1171400:intron 40.0%
TATAAGCACGTGTTATCCTG+GGG - chr8_1:1017953-1017972 Msa1171400:intron 40.0%
GTTATCCTGGGGAAAAAGAA+TGG - chr8_1:1017964-1017983 Msa1171400:intron 40.0%
GACCACTGTTAATTATGACG+CGG - chr8_1:1017986-1018005 Msa1171400:intron 40.0%
TACCGCGTCATAATTAACAG+TGG + chr8_1:1017991-1018010 None:intergenic 40.0%
! TTTTATTGTCTGAGCGACAG+AGG - chr8_1:1018056-1018075 Msa1171400:intron 40.0%
GAACTCTCTGCTAAGCATTT+GGG + chr8_1:1018140-1018159 None:intergenic 40.0%
AGAACTCTCTGCTAAGCATT+TGG + chr8_1:1018141-1018160 None:intergenic 40.0%
AGAGTTCTACTGCCAAAAAC+TGG - chr8_1:1018153-1018172 Msa1171400:intron 40.0%
GACACTAATGGAATGGAAAG+AGG - chr8_1:1018268-1018287 Msa1171400:intron 40.0%
AAAGAGGTGAGAACACTAGA+TGG - chr8_1:1018284-1018303 Msa1171400:intron 40.0%
TTCCCTAAAGTCCGTTTCTA+TGG - chr8_1:1018383-1018402 Msa1171400:CDS 40.0%
! CAGCCAATAAAGTCTTTTGG+TGG + chr8_1:1018512-1018531 None:intergenic 40.0%
CACCAAAAGACTTTATTGGC+TGG - chr8_1:1018510-1018529 Msa1171400:CDS 40.0%
AGTAGCATCTCCAATGTTGT+TGG - chr8_1:1018843-1018862 Msa1171400:CDS 40.0%
GTACTCAGTACCAACAACAT+TGG + chr8_1:1018856-1018875 None:intergenic 40.0%
TTCTTTAGTCTGTGCTGAAC+TGG - chr8_1:1019333-1019352 Msa1171400:CDS 40.0%
TGCCTGTTTGATCAAATGGT+TGG - chr8_1:1019399-1019418 Msa1171400:intron 40.0%
CCAAATGCTTCACTCAAACA+TGG + chr8_1:1019632-1019651 None:intergenic 40.0%
! CCATGTTTGAGTGAAGCATT+TGG - chr8_1:1019629-1019648 Msa1171400:intron 40.0%
AGAATCACAAAGAACTCGAG+AGG + chr8_1:1019661-1019680 None:intergenic 40.0%
CTCTAAACCTGTGACATCAA+AGG + chr8_1:1019700-1019719 None:intergenic 40.0%
! TTTCTCCTGCAATGCTTTTG+TGG - chr8_1:1019859-1019878 Msa1171400:intron 40.0%
!! ATGTTCCACAAAAGCATTGC+AGG + chr8_1:1019867-1019886 None:intergenic 40.0%
ACATTCTTCGCATGATGTCG+GGG - chr8_1:1016882-1016901 Msa1171400:three_prime_UTR 45.0%
GGAATTGTTCTTGTCTCGGT+TGG - chr8_1:1017524-1017543 Msa1171400:intron 45.0%
TTCTTGTCTCGGTTGGATGT+TGG - chr8_1:1017531-1017550 Msa1171400:intron 45.0%
TCTTGTCTCGGTTGGATGTT+GGG - chr8_1:1017532-1017551 Msa1171400:intron 45.0%
CTTCAGCTGTTTGCAAGAGA+TGG - chr8_1:1017568-1017587 Msa1171400:intron 45.0%
GTCGACTACACGTAAAGAAG+TGG + chr8_1:1017604-1017623 None:intergenic 45.0%
AATAGGTCTTACGCTGCACA+GGG + chr8_1:1017683-1017702 None:intergenic 45.0%
ATCCAACTCTGGCAACTCAA+GGG + chr8_1:1017706-1017725 None:intergenic 45.0%
TTGGTTATTGCTAAACCGGC+AGG - chr8_1:1017755-1017774 Msa1171400:intron 45.0%
CGTGATCAGATCCCTAGTAA+AGG + chr8_1:1017826-1017845 None:intergenic 45.0%
TGATGTTGGAGCACAAGAGA+AGG + chr8_1:1017883-1017902 None:intergenic 45.0%
TCTTGTGCTCCAACATCAAC+CGG - chr8_1:1017885-1017904 Msa1171400:intron 45.0%
GCTATAAGCACGTGTTATCC+TGG - chr8_1:1017951-1017970 Msa1171400:intron 45.0%
TTGACCCACTTGAACGTACT+TGG - chr8_1:1018093-1018112 Msa1171400:intron 45.0%
CAGTCCAAGTACGTTCAAGT+GGG + chr8_1:1018100-1018119 None:intergenic 45.0%
ACAGTCCAAGTACGTTCAAG+TGG + chr8_1:1018101-1018120 None:intergenic 45.0%
TACTGCCAAAAACTGGTCGA+AGG - chr8_1:1018160-1018179 Msa1171400:intron 45.0%
ACTGCCAAAAACTGGTCGAA+GGG - chr8_1:1018161-1018180 Msa1171400:intron 45.0%
! TTTCCCCTTCGACCAGTTTT+TGG + chr8_1:1018168-1018187 None:intergenic 45.0%
!! CATTCTAGGACTTACGTCAC+TGG - chr8_1:1018338-1018357 Msa1171400:CDS 45.0%
GCTCACTGGGATGATATCTA+CGG + chr8_1:1018439-1018458 None:intergenic 45.0%
! GTTTTCATCAACGCCTGCAA+GGG + chr8_1:1019261-1019280 None:intergenic 45.0%
TTCCAAACTATCGAGGATGG+AGG + chr8_1:1019291-1019310 None:intergenic 45.0%
ATGTGGTCCTTTGATGTCAC+AGG - chr8_1:1019690-1019709 Msa1171400:intron 45.0%
!!! TAATATTTTTTATAAAAAGT+TGG - chr8_1:1019179-1019198 Msa1171400:CDS 5.0%
!!! AATATTTTTTATAAAAAGTT+GGG - chr8_1:1019180-1019199 Msa1171400:CDS 5.0%
TTCGCATGATGTCGGGGAAA+AGG - chr8_1:1016888-1016907 Msa1171400:three_prime_UTR 50.0%
CAATAGAAGAGCTGCTGCTG+AGG - chr8_1:1017446-1017465 Msa1171400:intron 50.0%
CAAGACTTCAGCAGGGAGAT+CGG + chr8_1:1017505-1017524 None:intergenic 50.0%
CTTGTCTCGGTTGGATGTTG+GGG - chr8_1:1017533-1017552 Msa1171400:intron 50.0%
GTGTAGTCGACCAATCACCT+TGG - chr8_1:1017613-1017632 Msa1171400:intron 50.0%
GAATAGGTCTTACGCTGCAC+AGG + chr8_1:1017684-1017703 None:intergenic 50.0%
CTCTGGCAACTCAAGGGAAT+AGG + chr8_1:1017700-1017719 None:intergenic 50.0%
! TTCCCTTGAGTTGCCAGAGT+TGG - chr8_1:1017701-1017720 Msa1171400:intron 50.0%
CATCCAACTCTGGCAACTCA+AGG + chr8_1:1017707-1017726 None:intergenic 50.0%
! CTTGAGTTGCCAGAGTTGGA+TGG - chr8_1:1017705-1017724 Msa1171400:intron 50.0%
ACACGACATCCATCCAACTC+TGG + chr8_1:1017717-1017736 None:intergenic 50.0%
TATTGCTAAACCGGCAGGAC+TGG - chr8_1:1017760-1017779 Msa1171400:intron 50.0%
TCACGCTGCCAAAGTTTGAC+AGG - chr8_1:1017841-1017860 Msa1171400:intron 50.0%
! GTGGTCCATTCTTTTTCCCC+AGG + chr8_1:1017972-1017991 None:intergenic 50.0%
ATTGTCTGAGCGACAGAGGT+TGG - chr8_1:1018060-1018079 Msa1171400:intron 50.0%
CTCTCTGCTAAGCATTTGGG+CGG + chr8_1:1018137-1018156 None:intergenic 50.0%
CTGCCAAAAACTGGTCGAAG+GGG - chr8_1:1018162-1018181 Msa1171400:intron 50.0%
! GGTTTTCATCAACGCCTGCA+AGG + chr8_1:1019262-1019281 None:intergenic 50.0%
! TATCGAGGATGGAGGTGTGT+GGG + chr8_1:1019283-1019302 None:intergenic 50.0%
CACCTCCATCCTCGATAGTT+TGG - chr8_1:1019286-1019305 Msa1171400:CDS 50.0%
TAGTTCCTGCACGACAAGCA+GGG + chr8_1:1019581-1019600 None:intergenic 50.0%
CGATCTCCCTGCTGAAGTCT+TGG - chr8_1:1017503-1017522 Msa1171400:intron 55.0%
! TGAGCGACAGAGGTTGGCTA+GGG - chr8_1:1018066-1018085 Msa1171400:intron 55.0%
TCTGCTAAGCATTTGGGCGG+AGG + chr8_1:1018134-1018153 None:intergenic 55.0%
CTGTCACGACACTGCTCACT+GGG + chr8_1:1018452-1018471 None:intergenic 55.0%
CAGTGAGCAGTGTCGTGACA+GGG - chr8_1:1018451-1018470 Msa1171400:CDS 55.0%
! CTATCGAGGATGGAGGTGTG+TGG + chr8_1:1019284-1019303 None:intergenic 55.0%
CTAGTTCCTGCACGACAAGC+AGG + chr8_1:1019582-1019601 None:intergenic 55.0%
CTGCTTGTCGTGCAGGAACT+AGG - chr8_1:1019580-1019599 Msa1171400:intron 55.0%
TGCTAAACCGGCAGGACTGG+CGG - chr8_1:1017763-1017782 Msa1171400:intron 60.0%
! CTGAGCGACAGAGGTTGGCT+AGG - chr8_1:1018065-1018084 Msa1171400:intron 60.0%
! GAGCGACAGAGGTTGGCTAG+GGG - chr8_1:1018067-1018086 Msa1171400:intron 60.0%
CCTGTCACGACACTGCTCAC+TGG + chr8_1:1018453-1018472 None:intergenic 60.0%
CCAGTGAGCAGTGTCGTGAC+AGG - chr8_1:1018450-1018469 Msa1171400:CDS 60.0%
GACTTCCCTGCTTGTCGTGC+AGG - chr8_1:1019573-1019592 Msa1171400:intron 60.0%
! TAAACCGGCAGGACTGGCGG+AGG - chr8_1:1017766-1017785 Msa1171400:intron 65.0%
! CAGGACTGGCGGAGGTTGGA+CGG - chr8_1:1017774-1017793 Msa1171400:intron 65.0%
CCAACCTCCGCCAGTCCTGC+CGG + chr8_1:1017773-1017792 None:intergenic 70.0%
! CCGGCAGGACTGGCGGAGGT+TGG - chr8_1:1017770-1017789 Msa1171400:intron 75.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 1016860 1019994 1016860 ID=Msa1171400;Name=Msa1171400
chr8_1 mRNA 1016860 1019994 1016860 ID=Msa1171400-mRNA-1;Parent=Msa1171400;Name=Msa1171400-mRNA-1;_AED=0.02;_eAED=0.02;_QI=33|1|1|1|0.5|0.33|3|421|379
chr8_1 exon 1016860 1017259 1016860 ID=Msa1171400-mRNA-1:exon:354;Parent=Msa1171400-mRNA-1
chr8_1 exon 1018295 1019417 1018295 ID=Msa1171400-mRNA-1:exon:353;Parent=Msa1171400-mRNA-1
chr8_1 exon 1019924 1019994 1019924 ID=Msa1171400-mRNA-1:exon:352;Parent=Msa1171400-mRNA-1
chr8_1 five_prime_UTR 1019962 1019994 1019962 ID=Msa1171400-mRNA-1:five_prime_utr;Parent=Msa1171400-mRNA-1
chr8_1 CDS 1019924 1019961 1019924 ID=Msa1171400-mRNA-1:cds;Parent=Msa1171400-mRNA-1
chr8_1 CDS 1018316 1019417 1018316 ID=Msa1171400-mRNA-1:cds;Parent=Msa1171400-mRNA-1
chr8_1 three_prime_UTR 1018295 1018315 1018295 ID=Msa1171400-mRNA-1:three_prime_utr;Parent=Msa1171400-mRNA-1
chr8_1 three_prime_UTR 1016860 1017259 1016860 ID=Msa1171400-mRNA-1:three_prime_utr;Parent=Msa1171400-mRNA-1
Gene Sequence

>Msa1171400

ATGATGTCGGGGAAAAGGAAAAAAAAGTCGAAATCATCAATCAAAGACAATAGAAGAGCTGCTGCTGAGGTAAAAAGCAACAATTTGGAGCTGCAAACATGGGCCGATCTCCCTGCTGAAGTCTTGGAATTGTTCTTGTCTCGGTTGGATGTTGGGGATAATATTCGTGCTTCAGCTGTTTGCAAGAGATGGTGTTCAGTTGCCACTTCTTTACGTGTAGTCGACCAATCACCTTGGCTTATGTATTTCCCAAAAAAGGGTAATTGTTATGATTTCTATGACCCTGTGCAGCGTAAGACCTATTCCCTTGAGTTGCCAGAGTTGGATGGATGTCGTGTTTGCTATACAAAAGATGGTTGGTTATTGCTAAACCGGCAGGACTGGCGGAGGTTGGACGGAAATCACATTTTTTCTCTCTTTAATCCCTTTACTAGGGATCTGATCACGCTGCCAAAGTTTGACAGGACATATCAAATTGCTGCCTTCTCTTGTGCTCCAACATCAACCGGATGTGTTATATTAATTTTCAGACGTGTTGGTTCTAGTCTTGTAGCTATAAGCACGTGTTATCCTGGGGAAAAAGAATGGACCACTGTTAATTATGACGCGGTACTCTCATGTAGCATGTGCGATAAGCTTGTCTTTTCTAATGGGTTATTTTATTGTCTGAGCGACAGAGGTTGGCTAGGGGTGTTTGACCCACTTGAACGTACTTGGACTGTTCTCGAAGTACCTCCGCCCAAATGCTTAGCAGAGAGTTCTACTGCCAAAAACTGGTCGAAGGGGAAATTTATGATAGAGCACAAAGGAAATATATTTGTAGTTTATATATGTTGTGGTGAGGACCCAATAATTTTCAAGTTAGATCTGACACTAATGGAATGGAAAGAGGTGAGAACACTAGATGGAGTAACACTTTTTGCTAGTTTTTTGTCATCTCATTCTAGGACTTACGTCACTGGAATAATGAGAAACGGTGTCTACTTCCCTAAAGTCCGTTTCTATGGAAAACGTTGCATATCATTCTCTCTTGATGACCGTAGATATCATCCCAGTGAGCAGTGTCGTGACAGGGTAGAACCAAATACTTTTGAGAACTTCTGGATTGAACCACCAAAAGACTTTATTGGCTGGATGTAA
Protein sequence

>Msa1171400

MMSGKRKKKSKSSIKDNRRAAAEVKSNNLELQTWADLPAEVLELFLSRLDVGDNIRASAVCKRWCSVATSLRVVDQSPWLMYFPKKGNCYDFYDPVQRKTYSLELPELDGCRVCYTKDGWLLLNRQDWRRLDGNHIFSLFNPFTRDLITLPKFDRTYQIAAFSCAPTSTGCVILIFRRVGSSLVAISTCYPGEKEWTTVNYDAVLSCSMCDKLVFSNGLFYCLSDRGWLGVFDPLERTWTVLEVPPPKCLAESSTAKNWSKGKFMIEHKGNIFVVYICCGEDPIIFKLDLTLMEWKEVRTLDGVTLFASFLSSHSRTYVTGIMRNGVYFPKVRFYGKRCISFSLDDRRYHPSEQCRDRVEPNTFENFWIEPPKDFIGWM*