AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1125400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125400 MtrunA17_Chr7g0266441 95.515 379 15 1 1 379 1 377 0.0 725
Msa1125400 MtrunA17_Chr1g0178491 64.042 381 125 6 2 377 1 374 5.40e-173 487
Msa1125400 MtrunA17_Chr7g0266431 87.234 188 23 1 189 375 2 189 8.98e-120 344
Msa1125400 MtrunA17_Chr7g0266421 86.667 90 12 0 14 103 10 99 1.78e-48 160
Msa1125400 MtrunA17_Chr3g0143611 25.826 333 185 14 34 329 5 312 7.78e-14 72.0
Msa1125400 MtrunA17_Chr2g0294691 29.814 161 101 5 214 371 43 194 7.88e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125400 AT1G57790.1 44.789 355 182 6 24 374 3 347 3.45e-101 303
Msa1125400 AT3G56470.1 41.880 351 179 7 32 376 27 358 3.96e-82 255
Msa1125400 AT4G00893.1 39.489 352 198 8 33 378 44 386 2.06e-79 249
Msa1125400 AT4G10660.1 41.000 300 161 6 62 354 3 293 9.08e-68 217
Msa1125400 AT4G12370.1 38.835 309 170 8 81 379 1 300 1.48e-67 216
Msa1125400 AT1G49360.5 34.527 391 213 15 8 372 61 434 6.97e-52 179
Msa1125400 AT1G49360.1 34.527 391 213 15 8 372 84 457 1.31e-51 179
Msa1125400 AT1G49360.3 35.813 363 192 14 37 373 8 355 1.76e-51 176
Msa1125400 AT1G49360.2 35.813 363 192 14 37 373 8 355 1.76e-51 176
Msa1125400 AT1G49360.4 35.813 363 192 14 37 373 8 355 1.76e-51 176
Msa1125400 AT3G18720.1 34.085 355 189 11 36 374 51 376 2.35e-50 173
Msa1125400 AT4G10695.2 43.216 199 97 3 46 238 1 189 3.88e-48 163
Msa1125400 AT4G10695.1 43.216 199 97 3 46 238 1 189 3.88e-48 163
Msa1125400 AT4G12382.1 45.455 132 66 2 33 164 9 134 1.37e-35 127
Msa1125400 AT4G12382.2 45.455 132 66 2 33 164 9 134 1.37e-35 127
Msa1125400 AT2G44735.1 40.260 154 78 5 139 288 109 252 5.73e-23 99.4
Msa1125400 AT4G10700.1 41.270 126 66 3 258 376 119 243 1.19e-20 90.5
Msa1125400 AT4G10700.1 47.312 93 43 2 62 154 3 89 4.02e-20 89.0

Find 103 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATCTAACTTGAAAATTAT+TGG 0.158009 7_3:+76903641 None:intergenic
GAACCACCAAAAGACTTTAT+TGG 0.163822 7_3:-76903380 Msa1125400:CDS
CAGAAGTTCTCAAAAGTATT+TGG 0.184683 7_3:+76903407 None:intergenic
TTCCCTAAAGTCCGTTTCTA+TGG 0.238841 7_3:-76903503 Msa1125400:CDS
AGCTAAAATATTTATCTCTT+TGG 0.254305 7_3:-76902085 Msa1125400:three_prime_UTR
CCTATGTACAACCTTCAAAT+AGG 0.266334 7_3:+76902146 None:intergenic
TTTCCATAGAAACGGACTTT+AGG 0.272121 7_3:+76903500 None:intergenic
GATCTAACTTGAAAATTATT+GGG 0.325599 7_3:+76903642 None:intergenic
CTCTCTTTAATCCCTTTACT+AGG 0.326943 7_3:-76904075 Msa1125400:CDS
CAAGTTAGATCTGACACTAA+TGG 0.329403 7_3:-76903630 Msa1125400:CDS
ATCCAGCCAATAAAGTCTTT+TGG 0.339525 7_3:+76903374 None:intergenic
AGAACTCTCTGCTAAGCATT+TGG 0.351257 7_3:+76903748 None:intergenic
TTGACCCACTTGAACGTACT+TGG 0.354769 7_3:-76903793 Msa1125400:CDS
TTCCATAGAAACGGACTTTA+GGG 0.361226 7_3:+76903501 None:intergenic
AACATTCTTCGCATGATGTC+GGG 0.375799 7_3:-76905005 Msa1125400:exon
TCTTGTCTCGGTTGGATGTT+GGG 0.383783 7_3:-76904354 Msa1125400:CDS
CCATGTTTGAGTGAAGCATT+TGG 0.387770 7_3:-76902257 Msa1125400:three_prime_UTR
GCTATAAGCACGTGTTATCC+TGG 0.393896 7_3:-76903935 Msa1125400:CDS
ATGTTCCACAAAAGCATTGC+AGG 0.397891 7_3:+76902022 None:intergenic
GAACTCTCTGCTAAGCATTT+GGG 0.407425 7_3:+76903749 None:intergenic
ATGGTTGGTTATTGCTAAAC+CGG 0.409750 7_3:-76904135 Msa1125400:CDS
GGAATTGTTCTTGTCTCGGT+TGG 0.421431 7_3:-76904362 Msa1125400:CDS
GATTAGCAAAACCTATTTGA+AGG 0.423915 7_3:-76902157 Msa1125400:three_prime_UTR
TTCCCTTGAGTTGCCAGAGT+TGG 0.426018 7_3:-76904185 Msa1125400:CDS
CTGCTTGTCGTGCAGGAACT+AGG 0.426509 7_3:-76902306 Msa1125400:intron
AACAATTCCAAGACTTCAGC+AGG 0.429634 7_3:+76904376 None:intergenic
TTCTTGTCTCGGTTGGATGT+TGG 0.436225 7_3:-76904355 Msa1125400:CDS
TCTTGGAATTGTTCTTGTCT+CGG 0.438217 7_3:-76904366 Msa1125400:CDS
TCTTGTGCTCCAACATCAAC+CGG 0.442241 7_3:-76904001 Msa1125400:CDS
TATTGCTAAACCGGCAGGAC+TGG 0.447316 7_3:-76904126 Msa1125400:CDS
ACAATTTGGAGCTGCAAACA+TGG 0.451046 7_3:-76904408 Msa1125400:CDS
TTCGCATGATGTCGGGGAAA+AGG 0.455236 7_3:-76904998 Msa1125400:exon
CTCTGGCAACTCAAGGGAAT+AGG 0.460842 7_3:+76904189 None:intergenic
CGATCTCCCTGCTGAAGTCT+TGG 0.469551 7_3:-76904383 Msa1125400:CDS
AAAGAGGTGAGAACACTAGA+TGG 0.470190 7_3:-76903602 Msa1125400:CDS
GAATAGGTCTTACGCTGCAC+AGG 0.470767 7_3:+76904205 None:intergenic
TCTCTTTAATCCCTTTACTA+GGG 0.471216 7_3:-76904074 Msa1125400:CDS
ATGTGGTCCTTTGATGTCAC+AGG 0.471463 7_3:-76902196 Msa1125400:three_prime_UTR
TGATATGTCCTGTCAAACTT+TGG 0.477303 7_3:+76904037 None:intergenic
GAAAATTAATATAACACATC+CGG 0.478730 7_3:+76903982 None:intergenic
AATACATAAGCCAAGGTGAT+TGG 0.482476 7_3:+76904263 None:intergenic
CTTGAGTTGCCAGAGTTGGA+TGG 0.483726 7_3:-76904181 Msa1125400:CDS
CCTGTCACGACACTGCTCAC+TGG 0.484710 7_3:+76903436 None:intergenic
GTGATCAGATCCCTAGTAAA+GGG 0.491655 7_3:+76904064 None:intergenic
CTGTCACGACACTGCTCACT+GGG 0.495007 7_3:+76903437 None:intergenic
CACCAAAAGACTTTATTGGC+TGG 0.495682 7_3:-76903376 Msa1125400:CDS
CTGAGCGACAGAGGTTGGCT+AGG 0.495793 7_3:-76903821 Msa1125400:CDS
TGAGCGACAGAGGTTGGCTA+GGG 0.496901 7_3:-76903820 Msa1125400:CDS
CCAACCTCCGCCAGTCCTGC+CGG 0.497826 7_3:+76904116 None:intergenic
CCAGTGAGCAGTGTCGTGAC+AGG 0.497864 7_3:-76903436 Msa1125400:CDS
CGTGTTTGCTATACAAAAGA+TGG 0.502296 7_3:-76904154 Msa1125400:CDS
CTATAAGCACGTGTTATCCT+GGG 0.505414 7_3:-76903934 Msa1125400:CDS
CAGGACTGGCGGAGGTTGGA+CGG 0.511557 7_3:-76904112 Msa1125400:CDS
CTTCAGCTGTTTGCAAGAGA+TGG 0.513153 7_3:-76904318 Msa1125400:CDS
CCTATTTGAAGGTTGTACAT+AGG 0.514646 7_3:-76902146 Msa1125400:three_prime_UTR
CCGGCAGGACTGGCGGAGGT+TGG 0.523905 7_3:-76904116 Msa1125400:CDS
ATTGTCTGAGCGACAGAGGT+TGG 0.524197 7_3:-76903826 Msa1125400:CDS
CAAGACTTCAGCAGGGAGAT+CGG 0.525981 7_3:+76904384 None:intergenic
CATTCTAGGACTTACGTCAC+TGG 0.528125 7_3:-76903548 Msa1125400:CDS
TTGGTTATTGCTAAACCGGC+AGG 0.529301 7_3:-76904131 Msa1125400:CDS
CGTGATCAGATCCCTAGTAA+AGG 0.536285 7_3:+76904063 None:intergenic
TCACGCTGCCAAAGTTTGAC+AGG 0.539518 7_3:-76904045 Msa1125400:CDS
CCAAATGCTTCACTCAAACA+TGG 0.541352 7_3:+76902257 None:intergenic
GTCACTGGAATAATGAGAAA+CGG 0.542182 7_3:-76903533 Msa1125400:CDS
TAGATCTGACACTAATGGAA+TGG 0.542919 7_3:-76903625 Msa1125400:CDS
TCTGCTAAGCATTTGGGCGG+AGG 0.544418 7_3:+76903755 None:intergenic
CAACATTCTTCGCATGATGT+CGG 0.545714 7_3:-76905006 Msa1125400:exon
TTTGCTATACAAAAGATGGT+TGG 0.546454 7_3:-76904150 Msa1125400:CDS
CATCCAACTCTGGCAACTCA+AGG 0.553068 7_3:+76904182 None:intergenic
ATAACACATCCGGTTGATGT+TGG 0.553987 7_3:+76903992 None:intergenic
TTGAAATATTAAGAAATCAA+CGG 0.556920 7_3:+76901930 None:intergenic
TTTGGGAAATACATAAGCCA+AGG 0.561817 7_3:+76904256 None:intergenic
CATAGGAAGTTAATATGTGA+AGG 0.564276 7_3:-76902129 Msa1125400:three_prime_UTR
GACACTAATGGAATGGAAAG+AGG 0.564517 7_3:-76903618 Msa1125400:CDS
TGATGTTGGAGCACAAGAGA+AGG 0.568366 7_3:+76904006 None:intergenic
TGCTAAACCGGCAGGACTGG+CGG 0.569550 7_3:-76904123 Msa1125400:CDS
CAATAGAAGAGCTGCTGCTG+AGG 0.574535 7_3:-76904440 Msa1125400:CDS
ACACGACATCCATCCAACTC+TGG 0.576746 7_3:+76904172 None:intergenic
CTTGTCTCGGTTGGATGTTG+GGG 0.581584 7_3:-76904353 Msa1125400:CDS
CTCTAAACCTGTGACATCAA+AGG 0.581595 7_3:+76902189 None:intergenic
AGGAATGAATATCTGTGAGT+AGG 0.583262 7_3:-76902109 Msa1125400:three_prime_UTR
GTGATTCTTCATGCAAAATG+TGG 0.585218 7_3:-76902213 Msa1125400:three_prime_UTR
ATCCAACTCTGGCAACTCAA+GGG 0.590798 7_3:+76904183 None:intergenic
TAAACCGGCAGGACTGGCGG+AGG 0.593956 7_3:-76904120 Msa1125400:CDS
ACAGTCCAAGTACGTTCAAG+TGG 0.603382 7_3:+76903788 None:intergenic
CAATTTGGAGCTGCAAACAT+GGG 0.606485 7_3:-76904407 Msa1125400:CDS
GCTCACTGGGATGATATCTA+CGG 0.610400 7_3:+76903450 None:intergenic
GTCGACTACACGTAAAGAAG+TGG 0.612484 7_3:+76904285 None:intergenic
ACATTCTTCGCATGATGTCG+GGG 0.620719 7_3:-76905004 Msa1125400:exon
CAGTGAGCAGTGTCGTGACA+GGG 0.621223 7_3:-76903435 Msa1125400:CDS
AATAGGTCTTACGCTGCACA+GGG 0.638547 7_3:+76904206 None:intergenic
CTCTCTGCTAAGCATTTGGG+CGG 0.648219 7_3:+76903752 None:intergenic
TTTGTAGTTTATATATGTTG+TGG 0.650518 7_3:-76903671 Msa1125400:CDS
AGTTTATATATGTTGTGGTG+AGG 0.651124 7_3:-76903666 Msa1125400:CDS
AGAATCACAAAGAACTCGAG+AGG 0.661545 7_3:+76902228 None:intergenic
GAGCGACAGAGGTTGGCTAG+GGG 0.671202 7_3:-76903819 Msa1125400:CDS
CAGTCCAAGTACGTTCAAGT+GGG 0.682692 7_3:+76903789 None:intergenic
GACCACTGTTAATTATGACG+CGG 0.704466 7_3:-76903900 Msa1125400:CDS
TATAAGCACGTGTTATCCTG+GGG 0.707016 7_3:-76903933 Msa1125400:CDS
ACAATTCCAAGACTTCAGCA+GGG 0.713188 7_3:+76904377 None:intergenic
GTGTAGTCGACCAATCACCT+TGG 0.718766 7_3:-76904273 Msa1125400:CDS
TACCGCGTCATAATTAACAG+TGG 0.732690 7_3:+76903898 None:intergenic
AAATTTATGATAGAGCACAA+AGG 0.741593 7_3:-76903701 Msa1125400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATCTTATTTTCAAATA+AGG - chr7_3:76904496-76904515 Msa1125400:intron 10.0%
!!! ATATTTCAATTATATTTTTG+TGG - chr7_3:76905024-76905043 Msa1125400:five_prime_UTR 10.0%
!! CAATAATAATAATATGTATG+GGG + chr7_3:76902019-76902038 None:intergenic 15.0%
!! GCAATAATAATAATATGTAT+GGG + chr7_3:76902020-76902039 None:intergenic 15.0%
!! AGCAATAATAATAATATGTA+TGG + chr7_3:76902021-76902040 None:intergenic 15.0%
!! TACATATTATTATTATTGCT+AGG - chr7_3:76902021-76902040 Msa1125400:three_prime_UTR 15.0%
!! ACATATTATTATTATTGCTA+GGG - chr7_3:76902022-76902041 Msa1125400:three_prime_UTR 15.0%
!!! AATTTTGTTCAACAATTAAT+TGG - chr7_3:76902066-76902085 Msa1125400:three_prime_UTR 15.0%
!! TTTAGATTGATAAACATATT+TGG - chr7_3:76902344-76902363 Msa1125400:intron 15.0%
!! GATGTAAAAAAAAAAATTAG+CGG - chr7_3:76903586-76903605 Msa1125400:CDS 15.0%
!! TTGAAATATTAAGAAATCAA+CGG + chr7_3:76905013-76905032 None:intergenic 15.0%
!!! TCAATTTTTTGGCTTAATAA+TGG - chr7_3:76902100-76902119 Msa1125400:three_prime_UTR 20.0%
!! TATGATTTCTATATCCAATT+CGG - chr7_3:76902159-76902178 Msa1125400:three_prime_UTR 20.0%
!! GTTTCATCTATATTTCATAT+TGG - chr7_3:76902195-76902214 Msa1125400:three_prime_UTR 20.0%
!! GAAAATTAATATAACACATC+CGG + chr7_3:76902961-76902980 None:intergenic 20.0%
!! TTTGTAGTTTATATATGTTG+TGG - chr7_3:76903269-76903288 Msa1125400:intron 20.0%
!! GATCTAACTTGAAAATTATT+GGG + chr7_3:76903301-76903320 None:intergenic 20.0%
!! AGATCTAACTTGAAAATTAT+TGG + chr7_3:76903302-76903321 None:intergenic 20.0%
!! AGACTACATATTATAACTAT+TGG - chr7_3:76903670-76903689 Msa1125400:CDS 20.0%
!!! ATTTTTCATTTCCAAAACTA+AGG + chr7_3:76904013-76904032 None:intergenic 20.0%
!!! TTAGTTTTGGAAATGAAAAA+TGG - chr7_3:76904012-76904031 Msa1125400:CDS 20.0%
!!! TTATTTTCAAATAAGGACTT+GGG - chr7_3:76904503-76904522 Msa1125400:intron 20.0%
!! AGCTAAAATATTTATCTCTT+TGG - chr7_3:76904855-76904874 Msa1125400:intron 20.0%
! ATAATAATATGTATGGGGTT+AGG + chr7_3:76902014-76902033 None:intergenic 25.0%
!!! TTGCACTAACTTCAATTTTT+TGG - chr7_3:76902089-76902108 Msa1125400:three_prime_UTR 25.0%
! CATGAAAAATTTAACCGAAT+TGG + chr7_3:76902176-76902195 None:intergenic 25.0%
! TAGTTAATTCACAATAAAGC+TGG + chr7_3:76902232-76902251 None:intergenic 25.0%
!!! CATTTTTAAGTTGATGATTG+TGG - chr7_3:76902253-76902272 Msa1125400:three_prime_UTR 25.0%
! AGATTGATAAACATATTTGG+TGG - chr7_3:76902347-76902366 Msa1125400:intron 25.0%
! GATTGATAAACATATTTGGT+GGG - chr7_3:76902348-76902367 Msa1125400:intron 25.0%
! CTTATGTATTTCCCAAAAAA+GGG - chr7_3:76902690-76902709 Msa1125400:intron 25.0%
!!! CATAACAATTACCCTTTTTT+GGG + chr7_3:76902704-76902723 None:intergenic 25.0%
!!! TCATAACAATTACCCTTTTT+TGG + chr7_3:76902705-76902724 None:intergenic 25.0%
!! ATATTAATTTTCAGACGTGT+TGG - chr7_3:76902969-76902988 Msa1125400:intron 25.0%
!! ATAAGCTTGTCTTTTCTAAT+GGG - chr7_3:76903084-76903103 Msa1125400:intron 25.0%
! AAATTTATGATAGAGCACAA+AGG - chr7_3:76903239-76903258 Msa1125400:intron 25.0%
!!! TTGTGTAGAGCATTTATTTT+TGG - chr7_3:76903635-76903654 Msa1125400:CDS 25.0%
!! TATTGGATACATGCTTTATT+AGG - chr7_3:76903687-76903706 Msa1125400:CDS 25.0%
! TATTAGGCTGTAAACATTTA+TGG - chr7_3:76903703-76903722 Msa1125400:CDS 25.0%
! CAATAAGACTAGGAAAATTT+AGG + chr7_3:76903782-76903801 None:intergenic 25.0%
! TGAGTTATATGCAAATCTTA+AGG - chr7_3:76903849-76903868 Msa1125400:CDS 25.0%
!!! CACTAGTTTGTTGTAAAAAT+TGG - chr7_3:76904128-76904147 Msa1125400:CDS 25.0%
!! TTTAACTTCAATCGAGATTA+TGG + chr7_3:76904195-76904214 None:intergenic 25.0%
!! AGGGTATTCTATAAAATAAG+AGG + chr7_3:76904296-76904315 None:intergenic 25.0%
! GATAGTTTGGAAAATATAGT+CGG - chr7_3:76904353-76904372 Msa1125400:CDS 25.0%
!!! CTTATTTTCAAATAAGGACT+TGG - chr7_3:76904502-76904521 Msa1125400:intron 25.0%
! AGAAAAAGATATGATCAAAC+AGG + chr7_3:76904590-76904609 None:intergenic 25.0%
TTGTTGAACAAAATTGAGAG+TGG + chr7_3:76902061-76902080 None:intergenic 30.0%
AAAAAATGAAGTTAGCTAGC+AGG + chr7_3:76902415-76902434 None:intergenic 30.0%
TGAGGTAAAAAGCAACAATT+TGG - chr7_3:76902518-76902537 Msa1125400:intron 30.0%
GCTTATGTATTTCCCAAAAA+AGG - chr7_3:76902689-76902708 Msa1125400:intron 30.0%
! TTTGCTATACAAAAGATGGT+TGG - chr7_3:76902790-76902809 Msa1125400:intron 30.0%
TCTCTTTAATCCCTTTACTA+GGG - chr7_3:76902866-76902885 Msa1125400:intron 30.0%
! GATAAGCTTGTCTTTTCTAA+TGG - chr7_3:76903083-76903102 Msa1125400:intron 30.0%
AGTTTATATATGTTGTGGTG+AGG - chr7_3:76903274-76903293 Msa1125400:intron 30.0%
!!! GTTTTTTGTCATCTCATTCT+AGG - chr7_3:76903378-76903397 Msa1125400:CDS 30.0%
!! CAGAAGTTCTCAAAAGTATT+TGG + chr7_3:76903536-76903555 None:intergenic 30.0%
!! CAAATACTTTTGAGAACTTC+TGG - chr7_3:76903534-76903553 Msa1125400:CDS 30.0%
TTGTAGCTAACAATAAGACT+AGG + chr7_3:76903792-76903811 None:intergenic 30.0%
AAATCTTAAGGTTTCTCCAA+TGG - chr7_3:76903861-76903880 Msa1125400:CDS 30.0%
! ATGTTCATGATCCTTAGTTT+TGG - chr7_3:76903999-76904018 Msa1125400:CDS 30.0%
! ATTTTATAGAATACCCTTGC+AGG - chr7_3:76904299-76904318 Msa1125400:CDS 30.0%
! CTATATTTTCCAAACTATCG+AGG + chr7_3:76904352-76904371 None:intergenic 30.0%
ACTGGTATATGATTGCTAAA+TGG - chr7_3:76904405-76904424 Msa1125400:CDS 30.0%
! ATTTTGCCTGTTTGATCAAA+TGG - chr7_3:76904449-76904468 Msa1125400:CDS 30.0%
GATTAGCAAAACCTATTTGA+AGG - chr7_3:76904783-76904802 Msa1125400:intron 30.0%
CATAGGAAGTTAATATGTGA+AGG - chr7_3:76904811-76904830 Msa1125400:intron 30.0%
GGAGAAAAATCTTGAAATTC+AGG + chr7_3:76904900-76904919 None:intergenic 30.0%
ATATGTATGGGGTTAGGTTA+GGG + chr7_3:76902008-76902027 None:intergenic 35.0%
AATATGTATGGGGTTAGGTT+AGG + chr7_3:76902009-76902028 None:intergenic 35.0%
ATTCACAATAAAGCTGGTCA+TGG + chr7_3:76902226-76902245 None:intergenic 35.0%
GTAAACAGAACAGAAACAGT+GGG + chr7_3:76902309-76902328 None:intergenic 35.0%
AGTAAACAGAACAGAAACAG+TGG + chr7_3:76902310-76902329 None:intergenic 35.0%
! GATAAACATATTTGGTGGGA+TGG - chr7_3:76902352-76902371 Msa1125400:intron 35.0%
TCTTGGAATTGTTCTTGTCT+CGG - chr7_3:76902574-76902593 Msa1125400:intron 35.0%
AATACATAAGCCAAGGTGAT+TGG + chr7_3:76902680-76902699 None:intergenic 35.0%
TTTGGGAAATACATAAGCCA+AGG + chr7_3:76902687-76902706 None:intergenic 35.0%
!! CGTGTTTGCTATACAAAAGA+TGG - chr7_3:76902786-76902805 Msa1125400:intron 35.0%
! ATGGTTGGTTATTGCTAAAC+CGG - chr7_3:76902805-76902824 Msa1125400:intron 35.0%
CTCTCTTTAATCCCTTTACT+AGG - chr7_3:76902865-76902884 Msa1125400:intron 35.0%
TGATATGTCCTGTCAAACTT+TGG + chr7_3:76902906-76902925 None:intergenic 35.0%
CAAGTTAGATCTGACACTAA+TGG - chr7_3:76903310-76903329 Msa1125400:intron 35.0%
TAGATCTGACACTAATGGAA+TGG - chr7_3:76903315-76903334 Msa1125400:intron 35.0%
GTCACTGGAATAATGAGAAA+CGG - chr7_3:76903407-76903426 Msa1125400:CDS 35.0%
! TTCCATAGAAACGGACTTTA+GGG + chr7_3:76903442-76903461 None:intergenic 35.0%
! TTTCCATAGAAACGGACTTT+AGG + chr7_3:76903443-76903462 None:intergenic 35.0%
! TGCAACGTTTTCCATAGAAA+CGG + chr7_3:76903451-76903470 None:intergenic 35.0%
GAACCACCAAAAGACTTTAT+TGG - chr7_3:76903560-76903579 Msa1125400:CDS 35.0%
! ATCCAGCCAATAAAGTCTTT+TGG + chr7_3:76903569-76903588 None:intergenic 35.0%
GTAAACATTTATGGAGCTAC+TGG - chr7_3:76903712-76903731 Msa1125400:CDS 35.0%
GAATAAGATTGCATGCATCA+TGG + chr7_3:76903825-76903844 None:intergenic 35.0%
ACTAATGCACATGTAACCAT+TGG + chr7_3:76903880-76903899 None:intergenic 35.0%
! GACAGAGTACAATTTTGCTA+TGG - chr7_3:76903948-76903967 Msa1125400:CDS 35.0%
TGAAAAATGGTCAGAGTTAG+AGG - chr7_3:76904025-76904044 Msa1125400:CDS 35.0%
ACACACATGACTTTCTATGA+TGG + chr7_3:76904162-76904181 None:intergenic 35.0%
! ATTTTCCAAACTATCGAGGA+TGG + chr7_3:76904348-76904367 None:intergenic 35.0%
ATCCAACCATTTGATCAAAC+AGG + chr7_3:76904458-76904477 None:intergenic 35.0%
GTGATTCTTCATGCAAAATG+TGG - chr7_3:76904727-76904746 Msa1125400:intron 35.0%
CCTATGTACAACCTTCAAAT+AGG + chr7_3:76904797-76904816 None:intergenic 35.0%
CCTATTTGAAGGTTGTACAT+AGG - chr7_3:76904794-76904813 Msa1125400:intron 35.0%
AGGAATGAATATCTGTGAGT+AGG - chr7_3:76904831-76904850 Msa1125400:intron 35.0%
CAACATTCTTCGCATGATGT+CGG - chr7_3:76901934-76901953 Msa1125400:three_prime_UTR 40.0%
AACATTCTTCGCATGATGTC+GGG - chr7_3:76901935-76901954 Msa1125400:three_prime_UTR 40.0%
! ACAATTTGGAGCTGCAAACA+TGG - chr7_3:76902532-76902551 Msa1125400:intron 40.0%
! CAATTTGGAGCTGCAAACAT+GGG - chr7_3:76902533-76902552 Msa1125400:intron 40.0%
ACAATTCCAAGACTTCAGCA+GGG + chr7_3:76902566-76902585 None:intergenic 40.0%
AACAATTCCAAGACTTCAGC+AGG + chr7_3:76902567-76902586 None:intergenic 40.0%
GTGATCAGATCCCTAGTAAA+GGG + chr7_3:76902879-76902898 None:intergenic 40.0%
! ATAACACATCCGGTTGATGT+TGG + chr7_3:76902951-76902970 None:intergenic 40.0%
CTATAAGCACGTGTTATCCT+GGG - chr7_3:76903006-76903025 Msa1125400:intron 40.0%
TATAAGCACGTGTTATCCTG+GGG - chr7_3:76903007-76903026 Msa1125400:intron 40.0%
GTTATCCTGGGGAAAAAGAA+TGG - chr7_3:76903018-76903037 Msa1125400:intron 40.0%
GACCACTGTTAATTATGACG+CGG - chr7_3:76903040-76903059 Msa1125400:intron 40.0%
TACCGCGTCATAATTAACAG+TGG + chr7_3:76903045-76903064 None:intergenic 40.0%
! TTTTATTGTCTGAGCGACAG+AGG - chr7_3:76903110-76903129 Msa1125400:intron 40.0%
GAACTCTCTGCTAAGCATTT+GGG + chr7_3:76903194-76903213 None:intergenic 40.0%
AGAACTCTCTGCTAAGCATT+TGG + chr7_3:76903195-76903214 None:intergenic 40.0%
AGAGTTCTACTGCCAAAAAC+TGG - chr7_3:76903207-76903226 Msa1125400:intron 40.0%
GACACTAATGGAATGGAAAG+AGG - chr7_3:76903322-76903341 Msa1125400:intron 40.0%
AAAGAGGTGAGAACACTAGA+TGG - chr7_3:76903338-76903357 Msa1125400:intron 40.0%
TTCCCTAAAGTCCGTTTCTA+TGG - chr7_3:76903437-76903456 Msa1125400:CDS 40.0%
! CAGCCAATAAAGTCTTTTGG+TGG + chr7_3:76903566-76903585 None:intergenic 40.0%
CACCAAAAGACTTTATTGGC+TGG - chr7_3:76903564-76903583 Msa1125400:CDS 40.0%
AGTAGCATCTCCAATGTTGT+TGG - chr7_3:76903897-76903916 Msa1125400:CDS 40.0%
GTACTCAGTACCAACAACAT+TGG + chr7_3:76903910-76903929 None:intergenic 40.0%
TTCTTTAGTCTGTGCTGAAC+TGG - chr7_3:76904387-76904406 Msa1125400:CDS 40.0%
TGCCTGTTTGATCAAATGGT+TGG - chr7_3:76904453-76904472 Msa1125400:intron 40.0%
CCAAATGCTTCACTCAAACA+TGG + chr7_3:76904686-76904705 None:intergenic 40.0%
! CCATGTTTGAGTGAAGCATT+TGG - chr7_3:76904683-76904702 Msa1125400:intron 40.0%
AGAATCACAAAGAACTCGAG+AGG + chr7_3:76904715-76904734 None:intergenic 40.0%
CTCTAAACCTGTGACATCAA+AGG + chr7_3:76904754-76904773 None:intergenic 40.0%
! TTTCTCCTGCAATGCTTTTG+TGG - chr7_3:76904913-76904932 Msa1125400:intron 40.0%
!! ATGTTCCACAAAAGCATTGC+AGG + chr7_3:76904921-76904940 None:intergenic 40.0%
ACATTCTTCGCATGATGTCG+GGG - chr7_3:76901936-76901955 Msa1125400:three_prime_UTR 45.0%
GGAATTGTTCTTGTCTCGGT+TGG - chr7_3:76902578-76902597 Msa1125400:intron 45.0%
TTCTTGTCTCGGTTGGATGT+TGG - chr7_3:76902585-76902604 Msa1125400:intron 45.0%
TCTTGTCTCGGTTGGATGTT+GGG - chr7_3:76902586-76902605 Msa1125400:intron 45.0%
CTTCAGCTGTTTGCAAGAGA+TGG - chr7_3:76902622-76902641 Msa1125400:intron 45.0%
GTCGACTACACGTAAAGAAG+TGG + chr7_3:76902658-76902677 None:intergenic 45.0%
AATAGGTCTTACGCTGCACA+GGG + chr7_3:76902737-76902756 None:intergenic 45.0%
ATCCAACTCTGGCAACTCAA+GGG + chr7_3:76902760-76902779 None:intergenic 45.0%
TTGGTTATTGCTAAACCGGC+AGG - chr7_3:76902809-76902828 Msa1125400:intron 45.0%
CGTGATCAGATCCCTAGTAA+AGG + chr7_3:76902880-76902899 None:intergenic 45.0%
TGATGTTGGAGCACAAGAGA+AGG + chr7_3:76902937-76902956 None:intergenic 45.0%
TCTTGTGCTCCAACATCAAC+CGG - chr7_3:76902939-76902958 Msa1125400:intron 45.0%
GCTATAAGCACGTGTTATCC+TGG - chr7_3:76903005-76903024 Msa1125400:intron 45.0%
TTGACCCACTTGAACGTACT+TGG - chr7_3:76903147-76903166 Msa1125400:intron 45.0%
CAGTCCAAGTACGTTCAAGT+GGG + chr7_3:76903154-76903173 None:intergenic 45.0%
ACAGTCCAAGTACGTTCAAG+TGG + chr7_3:76903155-76903174 None:intergenic 45.0%
TACTGCCAAAAACTGGTCGA+AGG - chr7_3:76903214-76903233 Msa1125400:intron 45.0%
ACTGCCAAAAACTGGTCGAA+GGG - chr7_3:76903215-76903234 Msa1125400:intron 45.0%
! TTTCCCCTTCGACCAGTTTT+TGG + chr7_3:76903222-76903241 None:intergenic 45.0%
!! CATTCTAGGACTTACGTCAC+TGG - chr7_3:76903392-76903411 Msa1125400:CDS 45.0%
GCTCACTGGGATGATATCTA+CGG + chr7_3:76903493-76903512 None:intergenic 45.0%
! GTTTTCATCAACGCCTGCAA+GGG + chr7_3:76904315-76904334 None:intergenic 45.0%
TTCCAAACTATCGAGGATGG+AGG + chr7_3:76904345-76904364 None:intergenic 45.0%
ATGTGGTCCTTTGATGTCAC+AGG - chr7_3:76904744-76904763 Msa1125400:intron 45.0%
!!! TAATATTTTTTATAAAAAGT+TGG - chr7_3:76904233-76904252 Msa1125400:CDS 5.0%
!!! AATATTTTTTATAAAAAGTT+GGG - chr7_3:76904234-76904253 Msa1125400:CDS 5.0%
TTCGCATGATGTCGGGGAAA+AGG - chr7_3:76901942-76901961 Msa1125400:three_prime_UTR 50.0%
CAATAGAAGAGCTGCTGCTG+AGG - chr7_3:76902500-76902519 Msa1125400:intron 50.0%
CAAGACTTCAGCAGGGAGAT+CGG + chr7_3:76902559-76902578 None:intergenic 50.0%
CTTGTCTCGGTTGGATGTTG+GGG - chr7_3:76902587-76902606 Msa1125400:intron 50.0%
GTGTAGTCGACCAATCACCT+TGG - chr7_3:76902667-76902686 Msa1125400:intron 50.0%
GAATAGGTCTTACGCTGCAC+AGG + chr7_3:76902738-76902757 None:intergenic 50.0%
CTCTGGCAACTCAAGGGAAT+AGG + chr7_3:76902754-76902773 None:intergenic 50.0%
! TTCCCTTGAGTTGCCAGAGT+TGG - chr7_3:76902755-76902774 Msa1125400:intron 50.0%
CATCCAACTCTGGCAACTCA+AGG + chr7_3:76902761-76902780 None:intergenic 50.0%
! CTTGAGTTGCCAGAGTTGGA+TGG - chr7_3:76902759-76902778 Msa1125400:intron 50.0%
ACACGACATCCATCCAACTC+TGG + chr7_3:76902771-76902790 None:intergenic 50.0%
TATTGCTAAACCGGCAGGAC+TGG - chr7_3:76902814-76902833 Msa1125400:intron 50.0%
TCACGCTGCCAAAGTTTGAC+AGG - chr7_3:76902895-76902914 Msa1125400:intron 50.0%
! GTGGTCCATTCTTTTTCCCC+AGG + chr7_3:76903026-76903045 None:intergenic 50.0%
ATTGTCTGAGCGACAGAGGT+TGG - chr7_3:76903114-76903133 Msa1125400:intron 50.0%
CTCTCTGCTAAGCATTTGGG+CGG + chr7_3:76903191-76903210 None:intergenic 50.0%
CTGCCAAAAACTGGTCGAAG+GGG - chr7_3:76903216-76903235 Msa1125400:intron 50.0%
! GGTTTTCATCAACGCCTGCA+AGG + chr7_3:76904316-76904335 None:intergenic 50.0%
! TATCGAGGATGGAGGTGTGT+GGG + chr7_3:76904337-76904356 None:intergenic 50.0%
CACCTCCATCCTCGATAGTT+TGG - chr7_3:76904340-76904359 Msa1125400:CDS 50.0%
TAGTTCCTGCACGACAAGCA+GGG + chr7_3:76904635-76904654 None:intergenic 50.0%
CGATCTCCCTGCTGAAGTCT+TGG - chr7_3:76902557-76902576 Msa1125400:intron 55.0%
! TGAGCGACAGAGGTTGGCTA+GGG - chr7_3:76903120-76903139 Msa1125400:intron 55.0%
TCTGCTAAGCATTTGGGCGG+AGG + chr7_3:76903188-76903207 None:intergenic 55.0%
CTGTCACGACACTGCTCACT+GGG + chr7_3:76903506-76903525 None:intergenic 55.0%
CAGTGAGCAGTGTCGTGACA+GGG - chr7_3:76903505-76903524 Msa1125400:CDS 55.0%
! CTATCGAGGATGGAGGTGTG+TGG + chr7_3:76904338-76904357 None:intergenic 55.0%
CTAGTTCCTGCACGACAAGC+AGG + chr7_3:76904636-76904655 None:intergenic 55.0%
CTGCTTGTCGTGCAGGAACT+AGG - chr7_3:76904634-76904653 Msa1125400:intron 55.0%
TGCTAAACCGGCAGGACTGG+CGG - chr7_3:76902817-76902836 Msa1125400:intron 60.0%
! CTGAGCGACAGAGGTTGGCT+AGG - chr7_3:76903119-76903138 Msa1125400:intron 60.0%
! GAGCGACAGAGGTTGGCTAG+GGG - chr7_3:76903121-76903140 Msa1125400:intron 60.0%
CCTGTCACGACACTGCTCAC+TGG + chr7_3:76903507-76903526 None:intergenic 60.0%
CCAGTGAGCAGTGTCGTGAC+AGG - chr7_3:76903504-76903523 Msa1125400:CDS 60.0%
GACTTCCCTGCTTGTCGTGC+AGG - chr7_3:76904627-76904646 Msa1125400:intron 60.0%
! TAAACCGGCAGGACTGGCGG+AGG - chr7_3:76902820-76902839 Msa1125400:intron 65.0%
! CAGGACTGGCGGAGGTTGGA+CGG - chr7_3:76902828-76902847 Msa1125400:intron 65.0%
CCAACCTCCGCCAGTCCTGC+CGG + chr7_3:76902827-76902846 None:intergenic 70.0%
! CCGGCAGGACTGGCGGAGGT+TGG - chr7_3:76902824-76902843 Msa1125400:intron 75.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 76901914 76905048 76901914 ID=Msa1125400;Name=Msa1125400
chr7_3 mRNA 76901914 76905048 76901914 ID=Msa1125400-mRNA-1;Parent=Msa1125400;Name=Msa1125400-mRNA-1;_AED=0.02;_eAED=0.02;_QI=33|1|1|1|0.5|0.33|3|421|379
chr7_3 exon 76901914 76902313 76901914 ID=Msa1125400-mRNA-1:exon:21093;Parent=Msa1125400-mRNA-1
chr7_3 exon 76903349 76904471 76903349 ID=Msa1125400-mRNA-1:exon:21092;Parent=Msa1125400-mRNA-1
chr7_3 exon 76904978 76905048 76904978 ID=Msa1125400-mRNA-1:exon:21091;Parent=Msa1125400-mRNA-1
chr7_3 five_prime_UTR 76905016 76905048 76905016 ID=Msa1125400-mRNA-1:five_prime_utr;Parent=Msa1125400-mRNA-1
chr7_3 CDS 76904978 76905015 76904978 ID=Msa1125400-mRNA-1:cds;Parent=Msa1125400-mRNA-1
chr7_3 CDS 76903370 76904471 76903370 ID=Msa1125400-mRNA-1:cds;Parent=Msa1125400-mRNA-1
chr7_3 three_prime_UTR 76903349 76903369 76903349 ID=Msa1125400-mRNA-1:three_prime_utr;Parent=Msa1125400-mRNA-1
chr7_3 three_prime_UTR 76901914 76902313 76901914 ID=Msa1125400-mRNA-1:three_prime_utr;Parent=Msa1125400-mRNA-1
Gene Sequence

>Msa1125400

ATGATGTCGGGGAAAAGGAAAAAAAAGTCGAAATCATCAATCAAAGACAATAGAAGAGCTGCTGCTGAGGTAAAAAGCAACAATTTGGAGCTGCAAACATGGGCCGATCTCCCTGCTGAAGTCTTGGAATTGTTCTTGTCTCGGTTGGATGTTGGGGATAATATTCGTGCTTCAGCTGTTTGCAAGAGATGGTGTTCAGTTGCCACTTCTTTACGTGTAGTCGACCAATCACCTTGGCTTATGTATTTCCCAAAAAAGGGTAATTGTTATGATTTCTATGACCCTGTGCAGCGTAAGACCTATTCCCTTGAGTTGCCAGAGTTGGATGGATGTCGTGTTTGCTATACAAAAGATGGTTGGTTATTGCTAAACCGGCAGGACTGGCGGAGGTTGGACGGAAATCACATTTTTTCTCTCTTTAATCCCTTTACTAGGGATCTGATCACGCTGCCAAAGTTTGACAGGACATATCAAATTGCTGCCTTCTCTTGTGCTCCAACATCAACCGGATGTGTTATATTAATTTTCAGACGTGTTGGTTCTAGTCTTGTAGCTATAAGCACGTGTTATCCTGGGGAAAAAGAATGGACCACTGTTAATTATGACGCGGTACTCTCATGTAGCATGTGCGATAAGCTTGTCTTTTCTAATGGGTTATTTTATTGTCTGAGCGACAGAGGTTGGCTAGGGGTGTTTGACCCACTTGAACGTACTTGGACTGTTCTCGAAGTACCTCCGCCCAAATGCTTAGCAGAGAGTTCTACTGCCAAAAACTGGTCGAAGGGGAAATTTATGATAGAGCACAAAGGAAATATATTTGTAGTTTATATATGTTGTGGTGAGGACCCAATAATTTTCAAGTTAGATCTGACACTAATGGAATGGAAAGAGGTGAGAACACTAGATGGAGTAACACTTTTTGCTAGTTTTTTGTCATCTCATTCTAGGACTTACGTCACTGGAATAATGAGAAACGGTGTCTACTTCCCTAAAGTCCGTTTCTATGGAAAACGTTGCATATCATTCTCTCTTGATGACCGTAGATATCATCCCAGTGAGCAGTGTCGTGACAGGGTAGAACCAAATACTTTTGAGAACTTCTGGATTGAACCACCAAAAGACTTTATTGGCTGGATGTAA
Protein sequence

>Msa1125400

MMSGKRKKKSKSSIKDNRRAAAEVKSNNLELQTWADLPAEVLELFLSRLDVGDNIRASAVCKRWCSVATSLRVVDQSPWLMYFPKKGNCYDFYDPVQRKTYSLELPELDGCRVCYTKDGWLLLNRQDWRRLDGNHIFSLFNPFTRDLITLPKFDRTYQIAAFSCAPTSTGCVILIFRRVGSSLVAISTCYPGEKEWTTVNYDAVLSCSMCDKLVFSNGLFYCLSDRGWLGVFDPLERTWTVLEVPPPKCLAESSTAKNWSKGKFMIEHKGNIFVVYICCGEDPIIFKLDLTLMEWKEVRTLDGVTLFASFLSSHSRTYVTGIMRNGVYFPKVRFYGKRCISFSLDDRRYHPSEQCRDRVEPNTFENFWIEPPKDFIGWM*