AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1125680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125680 MtrunA17_Chr7g0266441 95.515 379 15 1 1 379 1 377 0.0 725
Msa1125680 MtrunA17_Chr1g0178491 64.042 381 125 6 2 377 1 374 5.40e-173 487
Msa1125680 MtrunA17_Chr7g0266431 87.234 188 23 1 189 375 2 189 8.98e-120 344
Msa1125680 MtrunA17_Chr7g0266421 86.667 90 12 0 14 103 10 99 1.78e-48 160
Msa1125680 MtrunA17_Chr3g0143611 25.826 333 185 14 34 329 5 312 7.78e-14 72.0
Msa1125680 MtrunA17_Chr2g0294691 29.814 161 101 5 214 371 43 194 7.88e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125680 AT1G57790.1 44.789 355 182 6 24 374 3 347 3.45e-101 303
Msa1125680 AT3G56470.1 41.880 351 179 7 32 376 27 358 3.96e-82 255
Msa1125680 AT4G00893.1 39.489 352 198 8 33 378 44 386 2.06e-79 249
Msa1125680 AT4G10660.1 41.000 300 161 6 62 354 3 293 9.08e-68 217
Msa1125680 AT4G12370.1 38.835 309 170 8 81 379 1 300 1.48e-67 216
Msa1125680 AT1G49360.5 34.527 391 213 15 8 372 61 434 6.97e-52 179
Msa1125680 AT1G49360.1 34.527 391 213 15 8 372 84 457 1.31e-51 179
Msa1125680 AT1G49360.3 35.813 363 192 14 37 373 8 355 1.76e-51 176
Msa1125680 AT1G49360.2 35.813 363 192 14 37 373 8 355 1.76e-51 176
Msa1125680 AT1G49360.4 35.813 363 192 14 37 373 8 355 1.76e-51 176
Msa1125680 AT3G18720.1 34.085 355 189 11 36 374 51 376 2.35e-50 173
Msa1125680 AT4G10695.2 43.216 199 97 3 46 238 1 189 3.88e-48 163
Msa1125680 AT4G10695.1 43.216 199 97 3 46 238 1 189 3.88e-48 163
Msa1125680 AT4G12382.1 45.455 132 66 2 33 164 9 134 1.37e-35 127
Msa1125680 AT4G12382.2 45.455 132 66 2 33 164 9 134 1.37e-35 127
Msa1125680 AT2G44735.1 40.260 154 78 5 139 288 109 252 5.73e-23 99.4
Msa1125680 AT4G10700.1 41.270 126 66 3 258 376 119 243 1.19e-20 90.5
Msa1125680 AT4G10700.1 47.312 93 43 2 62 154 3 89 4.02e-20 89.0

Find 103 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATCTAACTTGAAAATTAT+TGG 0.158009 7_3:+77134884 None:intergenic
GAACCACCAAAAGACTTTAT+TGG 0.163822 7_3:-77134623 Msa1125680:CDS
CAGAAGTTCTCAAAAGTATT+TGG 0.184683 7_3:+77134650 None:intergenic
TTCCCTAAAGTCCGTTTCTA+TGG 0.238841 7_3:-77134746 Msa1125680:CDS
AGCTAAAATATTTATCTCTT+TGG 0.254305 7_3:-77133328 Msa1125680:three_prime_UTR
CCTATGTACAACCTTCAAAT+AGG 0.266334 7_3:+77133389 None:intergenic
TTTCCATAGAAACGGACTTT+AGG 0.272121 7_3:+77134743 None:intergenic
GATCTAACTTGAAAATTATT+GGG 0.325599 7_3:+77134885 None:intergenic
CTCTCTTTAATCCCTTTACT+AGG 0.326943 7_3:-77135318 Msa1125680:CDS
CAAGTTAGATCTGACACTAA+TGG 0.329403 7_3:-77134873 Msa1125680:CDS
ATCCAGCCAATAAAGTCTTT+TGG 0.339525 7_3:+77134617 None:intergenic
AGAACTCTCTGCTAAGCATT+TGG 0.351257 7_3:+77134991 None:intergenic
TTGACCCACTTGAACGTACT+TGG 0.354769 7_3:-77135036 Msa1125680:CDS
TTCCATAGAAACGGACTTTA+GGG 0.361226 7_3:+77134744 None:intergenic
AACATTCTTCGCATGATGTC+GGG 0.375799 7_3:-77136248 Msa1125680:exon
TCTTGTCTCGGTTGGATGTT+GGG 0.383783 7_3:-77135597 Msa1125680:CDS
CCATGTTTGAGTGAAGCATT+TGG 0.387770 7_3:-77133500 Msa1125680:three_prime_UTR
GCTATAAGCACGTGTTATCC+TGG 0.393896 7_3:-77135178 Msa1125680:CDS
ATGTTCCACAAAAGCATTGC+AGG 0.397891 7_3:+77133265 None:intergenic
GAACTCTCTGCTAAGCATTT+GGG 0.407425 7_3:+77134992 None:intergenic
ATGGTTGGTTATTGCTAAAC+CGG 0.409750 7_3:-77135378 Msa1125680:CDS
GGAATTGTTCTTGTCTCGGT+TGG 0.421431 7_3:-77135605 Msa1125680:CDS
GATTAGCAAAACCTATTTGA+AGG 0.423915 7_3:-77133400 Msa1125680:three_prime_UTR
TTCCCTTGAGTTGCCAGAGT+TGG 0.426018 7_3:-77135428 Msa1125680:CDS
CTGCTTGTCGTGCAGGAACT+AGG 0.426509 7_3:-77133549 Msa1125680:intron
AACAATTCCAAGACTTCAGC+AGG 0.429634 7_3:+77135619 None:intergenic
TTCTTGTCTCGGTTGGATGT+TGG 0.436225 7_3:-77135598 Msa1125680:CDS
TCTTGGAATTGTTCTTGTCT+CGG 0.438217 7_3:-77135609 Msa1125680:CDS
TCTTGTGCTCCAACATCAAC+CGG 0.442241 7_3:-77135244 Msa1125680:CDS
TATTGCTAAACCGGCAGGAC+TGG 0.447316 7_3:-77135369 Msa1125680:CDS
ACAATTTGGAGCTGCAAACA+TGG 0.451046 7_3:-77135651 Msa1125680:CDS
TTCGCATGATGTCGGGGAAA+AGG 0.455236 7_3:-77136241 Msa1125680:exon
CTCTGGCAACTCAAGGGAAT+AGG 0.460842 7_3:+77135432 None:intergenic
CGATCTCCCTGCTGAAGTCT+TGG 0.469551 7_3:-77135626 Msa1125680:CDS
AAAGAGGTGAGAACACTAGA+TGG 0.470190 7_3:-77134845 Msa1125680:CDS
GAATAGGTCTTACGCTGCAC+AGG 0.470767 7_3:+77135448 None:intergenic
TCTCTTTAATCCCTTTACTA+GGG 0.471216 7_3:-77135317 Msa1125680:CDS
ATGTGGTCCTTTGATGTCAC+AGG 0.471463 7_3:-77133439 Msa1125680:three_prime_UTR
TGATATGTCCTGTCAAACTT+TGG 0.477303 7_3:+77135280 None:intergenic
GAAAATTAATATAACACATC+CGG 0.478730 7_3:+77135225 None:intergenic
AATACATAAGCCAAGGTGAT+TGG 0.482476 7_3:+77135506 None:intergenic
CTTGAGTTGCCAGAGTTGGA+TGG 0.483726 7_3:-77135424 Msa1125680:CDS
CCTGTCACGACACTGCTCAC+TGG 0.484710 7_3:+77134679 None:intergenic
GTGATCAGATCCCTAGTAAA+GGG 0.491655 7_3:+77135307 None:intergenic
CTGTCACGACACTGCTCACT+GGG 0.495007 7_3:+77134680 None:intergenic
CACCAAAAGACTTTATTGGC+TGG 0.495682 7_3:-77134619 Msa1125680:CDS
CTGAGCGACAGAGGTTGGCT+AGG 0.495793 7_3:-77135064 Msa1125680:CDS
TGAGCGACAGAGGTTGGCTA+GGG 0.496901 7_3:-77135063 Msa1125680:CDS
CCAACCTCCGCCAGTCCTGC+CGG 0.497826 7_3:+77135359 None:intergenic
CCAGTGAGCAGTGTCGTGAC+AGG 0.497864 7_3:-77134679 Msa1125680:CDS
CGTGTTTGCTATACAAAAGA+TGG 0.502296 7_3:-77135397 Msa1125680:CDS
CTATAAGCACGTGTTATCCT+GGG 0.505414 7_3:-77135177 Msa1125680:CDS
CAGGACTGGCGGAGGTTGGA+CGG 0.511557 7_3:-77135355 Msa1125680:CDS
CTTCAGCTGTTTGCAAGAGA+TGG 0.513153 7_3:-77135561 Msa1125680:CDS
CCTATTTGAAGGTTGTACAT+AGG 0.514646 7_3:-77133389 Msa1125680:three_prime_UTR
CCGGCAGGACTGGCGGAGGT+TGG 0.523905 7_3:-77135359 Msa1125680:CDS
ATTGTCTGAGCGACAGAGGT+TGG 0.524197 7_3:-77135069 Msa1125680:CDS
CAAGACTTCAGCAGGGAGAT+CGG 0.525981 7_3:+77135627 None:intergenic
CATTCTAGGACTTACGTCAC+TGG 0.528125 7_3:-77134791 Msa1125680:CDS
TTGGTTATTGCTAAACCGGC+AGG 0.529301 7_3:-77135374 Msa1125680:CDS
CGTGATCAGATCCCTAGTAA+AGG 0.536285 7_3:+77135306 None:intergenic
TCACGCTGCCAAAGTTTGAC+AGG 0.539518 7_3:-77135288 Msa1125680:CDS
CCAAATGCTTCACTCAAACA+TGG 0.541352 7_3:+77133500 None:intergenic
GTCACTGGAATAATGAGAAA+CGG 0.542182 7_3:-77134776 Msa1125680:CDS
TAGATCTGACACTAATGGAA+TGG 0.542919 7_3:-77134868 Msa1125680:CDS
TCTGCTAAGCATTTGGGCGG+AGG 0.544418 7_3:+77134998 None:intergenic
CAACATTCTTCGCATGATGT+CGG 0.545714 7_3:-77136249 Msa1125680:exon
TTTGCTATACAAAAGATGGT+TGG 0.546454 7_3:-77135393 Msa1125680:CDS
CATCCAACTCTGGCAACTCA+AGG 0.553068 7_3:+77135425 None:intergenic
ATAACACATCCGGTTGATGT+TGG 0.553987 7_3:+77135235 None:intergenic
TTGAAATATTAAGAAATCAA+CGG 0.556920 7_3:+77133173 None:intergenic
TTTGGGAAATACATAAGCCA+AGG 0.561817 7_3:+77135499 None:intergenic
CATAGGAAGTTAATATGTGA+AGG 0.564276 7_3:-77133372 Msa1125680:three_prime_UTR
GACACTAATGGAATGGAAAG+AGG 0.564517 7_3:-77134861 Msa1125680:CDS
TGATGTTGGAGCACAAGAGA+AGG 0.568366 7_3:+77135249 None:intergenic
TGCTAAACCGGCAGGACTGG+CGG 0.569550 7_3:-77135366 Msa1125680:CDS
CAATAGAAGAGCTGCTGCTG+AGG 0.574535 7_3:-77135683 Msa1125680:CDS
ACACGACATCCATCCAACTC+TGG 0.576746 7_3:+77135415 None:intergenic
CTTGTCTCGGTTGGATGTTG+GGG 0.581584 7_3:-77135596 Msa1125680:CDS
CTCTAAACCTGTGACATCAA+AGG 0.581595 7_3:+77133432 None:intergenic
AGGAATGAATATCTGTGAGT+AGG 0.583262 7_3:-77133352 Msa1125680:three_prime_UTR
GTGATTCTTCATGCAAAATG+TGG 0.585218 7_3:-77133456 Msa1125680:three_prime_UTR
ATCCAACTCTGGCAACTCAA+GGG 0.590798 7_3:+77135426 None:intergenic
TAAACCGGCAGGACTGGCGG+AGG 0.593956 7_3:-77135363 Msa1125680:CDS
ACAGTCCAAGTACGTTCAAG+TGG 0.603382 7_3:+77135031 None:intergenic
CAATTTGGAGCTGCAAACAT+GGG 0.606485 7_3:-77135650 Msa1125680:CDS
GCTCACTGGGATGATATCTA+CGG 0.610400 7_3:+77134693 None:intergenic
GTCGACTACACGTAAAGAAG+TGG 0.612484 7_3:+77135528 None:intergenic
ACATTCTTCGCATGATGTCG+GGG 0.620719 7_3:-77136247 Msa1125680:exon
CAGTGAGCAGTGTCGTGACA+GGG 0.621223 7_3:-77134678 Msa1125680:CDS
AATAGGTCTTACGCTGCACA+GGG 0.638547 7_3:+77135449 None:intergenic
CTCTCTGCTAAGCATTTGGG+CGG 0.648219 7_3:+77134995 None:intergenic
TTTGTAGTTTATATATGTTG+TGG 0.650518 7_3:-77134914 Msa1125680:CDS
AGTTTATATATGTTGTGGTG+AGG 0.651124 7_3:-77134909 Msa1125680:CDS
AGAATCACAAAGAACTCGAG+AGG 0.661545 7_3:+77133471 None:intergenic
GAGCGACAGAGGTTGGCTAG+GGG 0.671202 7_3:-77135062 Msa1125680:CDS
CAGTCCAAGTACGTTCAAGT+GGG 0.682692 7_3:+77135032 None:intergenic
GACCACTGTTAATTATGACG+CGG 0.704466 7_3:-77135143 Msa1125680:CDS
TATAAGCACGTGTTATCCTG+GGG 0.707016 7_3:-77135176 Msa1125680:CDS
ACAATTCCAAGACTTCAGCA+GGG 0.713188 7_3:+77135620 None:intergenic
GTGTAGTCGACCAATCACCT+TGG 0.718766 7_3:-77135516 Msa1125680:CDS
TACCGCGTCATAATTAACAG+TGG 0.732690 7_3:+77135141 None:intergenic
AAATTTATGATAGAGCACAA+AGG 0.741593 7_3:-77134944 Msa1125680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATCTTATTTTCAAATA+AGG - chr7_3:77135739-77135758 Msa1125680:intron 10.0%
!!! ATATTTCAATTATATTTTTG+TGG - chr7_3:77136267-77136286 Msa1125680:five_prime_UTR 10.0%
!! CAATAATAATAATATGTATG+GGG + chr7_3:77133262-77133281 None:intergenic 15.0%
!! GCAATAATAATAATATGTAT+GGG + chr7_3:77133263-77133282 None:intergenic 15.0%
!! AGCAATAATAATAATATGTA+TGG + chr7_3:77133264-77133283 None:intergenic 15.0%
!! TACATATTATTATTATTGCT+AGG - chr7_3:77133264-77133283 Msa1125680:three_prime_UTR 15.0%
!! ACATATTATTATTATTGCTA+GGG - chr7_3:77133265-77133284 Msa1125680:three_prime_UTR 15.0%
!!! AATTTTGTTCAACAATTAAT+TGG - chr7_3:77133309-77133328 Msa1125680:three_prime_UTR 15.0%
!! TTTAGATTGATAAACATATT+TGG - chr7_3:77133587-77133606 Msa1125680:intron 15.0%
!! GATGTAAAAAAAAAAATTAG+CGG - chr7_3:77134829-77134848 Msa1125680:CDS 15.0%
!! TTGAAATATTAAGAAATCAA+CGG + chr7_3:77136256-77136275 None:intergenic 15.0%
!!! TCAATTTTTTGGCTTAATAA+TGG - chr7_3:77133343-77133362 Msa1125680:three_prime_UTR 20.0%
!! TATGATTTCTATATCCAATT+CGG - chr7_3:77133402-77133421 Msa1125680:three_prime_UTR 20.0%
!! GTTTCATCTATATTTCATAT+TGG - chr7_3:77133438-77133457 Msa1125680:three_prime_UTR 20.0%
!! GAAAATTAATATAACACATC+CGG + chr7_3:77134204-77134223 None:intergenic 20.0%
!! TTTGTAGTTTATATATGTTG+TGG - chr7_3:77134512-77134531 Msa1125680:intron 20.0%
!! GATCTAACTTGAAAATTATT+GGG + chr7_3:77134544-77134563 None:intergenic 20.0%
!! AGATCTAACTTGAAAATTAT+TGG + chr7_3:77134545-77134564 None:intergenic 20.0%
!! AGACTACATATTATAACTAT+TGG - chr7_3:77134913-77134932 Msa1125680:CDS 20.0%
!!! ATTTTTCATTTCCAAAACTA+AGG + chr7_3:77135256-77135275 None:intergenic 20.0%
!!! TTAGTTTTGGAAATGAAAAA+TGG - chr7_3:77135255-77135274 Msa1125680:CDS 20.0%
!!! TTATTTTCAAATAAGGACTT+GGG - chr7_3:77135746-77135765 Msa1125680:intron 20.0%
!! AGCTAAAATATTTATCTCTT+TGG - chr7_3:77136098-77136117 Msa1125680:intron 20.0%
! ATAATAATATGTATGGGGTT+AGG + chr7_3:77133257-77133276 None:intergenic 25.0%
!!! TTGCACTAACTTCAATTTTT+TGG - chr7_3:77133332-77133351 Msa1125680:three_prime_UTR 25.0%
! CATGAAAAATTTAACCGAAT+TGG + chr7_3:77133419-77133438 None:intergenic 25.0%
! TAGTTAATTCACAATAAAGC+TGG + chr7_3:77133475-77133494 None:intergenic 25.0%
!!! CATTTTTAAGTTGATGATTG+TGG - chr7_3:77133496-77133515 Msa1125680:three_prime_UTR 25.0%
! AGATTGATAAACATATTTGG+TGG - chr7_3:77133590-77133609 Msa1125680:intron 25.0%
! GATTGATAAACATATTTGGT+GGG - chr7_3:77133591-77133610 Msa1125680:intron 25.0%
! CTTATGTATTTCCCAAAAAA+GGG - chr7_3:77133933-77133952 Msa1125680:intron 25.0%
!!! CATAACAATTACCCTTTTTT+GGG + chr7_3:77133947-77133966 None:intergenic 25.0%
!!! TCATAACAATTACCCTTTTT+TGG + chr7_3:77133948-77133967 None:intergenic 25.0%
!! ATATTAATTTTCAGACGTGT+TGG - chr7_3:77134212-77134231 Msa1125680:intron 25.0%
!! ATAAGCTTGTCTTTTCTAAT+GGG - chr7_3:77134327-77134346 Msa1125680:intron 25.0%
! AAATTTATGATAGAGCACAA+AGG - chr7_3:77134482-77134501 Msa1125680:intron 25.0%
!!! TTGTGTAGAGCATTTATTTT+TGG - chr7_3:77134878-77134897 Msa1125680:CDS 25.0%
!! TATTGGATACATGCTTTATT+AGG - chr7_3:77134930-77134949 Msa1125680:CDS 25.0%
! TATTAGGCTGTAAACATTTA+TGG - chr7_3:77134946-77134965 Msa1125680:CDS 25.0%
! CAATAAGACTAGGAAAATTT+AGG + chr7_3:77135025-77135044 None:intergenic 25.0%
! TGAGTTATATGCAAATCTTA+AGG - chr7_3:77135092-77135111 Msa1125680:CDS 25.0%
!!! CACTAGTTTGTTGTAAAAAT+TGG - chr7_3:77135371-77135390 Msa1125680:CDS 25.0%
!! TTTAACTTCAATCGAGATTA+TGG + chr7_3:77135438-77135457 None:intergenic 25.0%
!! AGGGTATTCTATAAAATAAG+AGG + chr7_3:77135539-77135558 None:intergenic 25.0%
! GATAGTTTGGAAAATATAGT+CGG - chr7_3:77135596-77135615 Msa1125680:CDS 25.0%
!!! CTTATTTTCAAATAAGGACT+TGG - chr7_3:77135745-77135764 Msa1125680:intron 25.0%
! AGAAAAAGATATGATCAAAC+AGG + chr7_3:77135833-77135852 None:intergenic 25.0%
TTGTTGAACAAAATTGAGAG+TGG + chr7_3:77133304-77133323 None:intergenic 30.0%
AAAAAATGAAGTTAGCTAGC+AGG + chr7_3:77133658-77133677 None:intergenic 30.0%
TGAGGTAAAAAGCAACAATT+TGG - chr7_3:77133761-77133780 Msa1125680:intron 30.0%
GCTTATGTATTTCCCAAAAA+AGG - chr7_3:77133932-77133951 Msa1125680:intron 30.0%
! TTTGCTATACAAAAGATGGT+TGG - chr7_3:77134033-77134052 Msa1125680:intron 30.0%
TCTCTTTAATCCCTTTACTA+GGG - chr7_3:77134109-77134128 Msa1125680:intron 30.0%
! GATAAGCTTGTCTTTTCTAA+TGG - chr7_3:77134326-77134345 Msa1125680:intron 30.0%
AGTTTATATATGTTGTGGTG+AGG - chr7_3:77134517-77134536 Msa1125680:intron 30.0%
!!! GTTTTTTGTCATCTCATTCT+AGG - chr7_3:77134621-77134640 Msa1125680:CDS 30.0%
!! CAGAAGTTCTCAAAAGTATT+TGG + chr7_3:77134779-77134798 None:intergenic 30.0%
!! CAAATACTTTTGAGAACTTC+TGG - chr7_3:77134777-77134796 Msa1125680:CDS 30.0%
TTGTAGCTAACAATAAGACT+AGG + chr7_3:77135035-77135054 None:intergenic 30.0%
AAATCTTAAGGTTTCTCCAA+TGG - chr7_3:77135104-77135123 Msa1125680:CDS 30.0%
! ATGTTCATGATCCTTAGTTT+TGG - chr7_3:77135242-77135261 Msa1125680:CDS 30.0%
! ATTTTATAGAATACCCTTGC+AGG - chr7_3:77135542-77135561 Msa1125680:CDS 30.0%
! CTATATTTTCCAAACTATCG+AGG + chr7_3:77135595-77135614 None:intergenic 30.0%
ACTGGTATATGATTGCTAAA+TGG - chr7_3:77135648-77135667 Msa1125680:CDS 30.0%
! ATTTTGCCTGTTTGATCAAA+TGG - chr7_3:77135692-77135711 Msa1125680:CDS 30.0%
GATTAGCAAAACCTATTTGA+AGG - chr7_3:77136026-77136045 Msa1125680:intron 30.0%
CATAGGAAGTTAATATGTGA+AGG - chr7_3:77136054-77136073 Msa1125680:intron 30.0%
GGAGAAAAATCTTGAAATTC+AGG + chr7_3:77136143-77136162 None:intergenic 30.0%
ATATGTATGGGGTTAGGTTA+GGG + chr7_3:77133251-77133270 None:intergenic 35.0%
AATATGTATGGGGTTAGGTT+AGG + chr7_3:77133252-77133271 None:intergenic 35.0%
ATTCACAATAAAGCTGGTCA+TGG + chr7_3:77133469-77133488 None:intergenic 35.0%
GTAAACAGAACAGAAACAGT+GGG + chr7_3:77133552-77133571 None:intergenic 35.0%
AGTAAACAGAACAGAAACAG+TGG + chr7_3:77133553-77133572 None:intergenic 35.0%
! GATAAACATATTTGGTGGGA+TGG - chr7_3:77133595-77133614 Msa1125680:intron 35.0%
TCTTGGAATTGTTCTTGTCT+CGG - chr7_3:77133817-77133836 Msa1125680:intron 35.0%
AATACATAAGCCAAGGTGAT+TGG + chr7_3:77133923-77133942 None:intergenic 35.0%
TTTGGGAAATACATAAGCCA+AGG + chr7_3:77133930-77133949 None:intergenic 35.0%
!! CGTGTTTGCTATACAAAAGA+TGG - chr7_3:77134029-77134048 Msa1125680:intron 35.0%
! ATGGTTGGTTATTGCTAAAC+CGG - chr7_3:77134048-77134067 Msa1125680:intron 35.0%
CTCTCTTTAATCCCTTTACT+AGG - chr7_3:77134108-77134127 Msa1125680:intron 35.0%
TGATATGTCCTGTCAAACTT+TGG + chr7_3:77134149-77134168 None:intergenic 35.0%
CAAGTTAGATCTGACACTAA+TGG - chr7_3:77134553-77134572 Msa1125680:intron 35.0%
TAGATCTGACACTAATGGAA+TGG - chr7_3:77134558-77134577 Msa1125680:intron 35.0%
GTCACTGGAATAATGAGAAA+CGG - chr7_3:77134650-77134669 Msa1125680:CDS 35.0%
! TTCCATAGAAACGGACTTTA+GGG + chr7_3:77134685-77134704 None:intergenic 35.0%
! TTTCCATAGAAACGGACTTT+AGG + chr7_3:77134686-77134705 None:intergenic 35.0%
! TGCAACGTTTTCCATAGAAA+CGG + chr7_3:77134694-77134713 None:intergenic 35.0%
GAACCACCAAAAGACTTTAT+TGG - chr7_3:77134803-77134822 Msa1125680:CDS 35.0%
! ATCCAGCCAATAAAGTCTTT+TGG + chr7_3:77134812-77134831 None:intergenic 35.0%
GTAAACATTTATGGAGCTAC+TGG - chr7_3:77134955-77134974 Msa1125680:CDS 35.0%
GAATAAGATTGCATGCATCA+TGG + chr7_3:77135068-77135087 None:intergenic 35.0%
ACTAATGCACATGTAACCAT+TGG + chr7_3:77135123-77135142 None:intergenic 35.0%
! GACAGAGTACAATTTTGCTA+TGG - chr7_3:77135191-77135210 Msa1125680:CDS 35.0%
TGAAAAATGGTCAGAGTTAG+AGG - chr7_3:77135268-77135287 Msa1125680:CDS 35.0%
ACACACATGACTTTCTATGA+TGG + chr7_3:77135405-77135424 None:intergenic 35.0%
! ATTTTCCAAACTATCGAGGA+TGG + chr7_3:77135591-77135610 None:intergenic 35.0%
ATCCAACCATTTGATCAAAC+AGG + chr7_3:77135701-77135720 None:intergenic 35.0%
GTGATTCTTCATGCAAAATG+TGG - chr7_3:77135970-77135989 Msa1125680:intron 35.0%
CCTATGTACAACCTTCAAAT+AGG + chr7_3:77136040-77136059 None:intergenic 35.0%
CCTATTTGAAGGTTGTACAT+AGG - chr7_3:77136037-77136056 Msa1125680:intron 35.0%
AGGAATGAATATCTGTGAGT+AGG - chr7_3:77136074-77136093 Msa1125680:intron 35.0%
CAACATTCTTCGCATGATGT+CGG - chr7_3:77133177-77133196 Msa1125680:three_prime_UTR 40.0%
AACATTCTTCGCATGATGTC+GGG - chr7_3:77133178-77133197 Msa1125680:three_prime_UTR 40.0%
! ACAATTTGGAGCTGCAAACA+TGG - chr7_3:77133775-77133794 Msa1125680:intron 40.0%
! CAATTTGGAGCTGCAAACAT+GGG - chr7_3:77133776-77133795 Msa1125680:intron 40.0%
ACAATTCCAAGACTTCAGCA+GGG + chr7_3:77133809-77133828 None:intergenic 40.0%
AACAATTCCAAGACTTCAGC+AGG + chr7_3:77133810-77133829 None:intergenic 40.0%
GTGATCAGATCCCTAGTAAA+GGG + chr7_3:77134122-77134141 None:intergenic 40.0%
! ATAACACATCCGGTTGATGT+TGG + chr7_3:77134194-77134213 None:intergenic 40.0%
CTATAAGCACGTGTTATCCT+GGG - chr7_3:77134249-77134268 Msa1125680:intron 40.0%
TATAAGCACGTGTTATCCTG+GGG - chr7_3:77134250-77134269 Msa1125680:intron 40.0%
GTTATCCTGGGGAAAAAGAA+TGG - chr7_3:77134261-77134280 Msa1125680:intron 40.0%
GACCACTGTTAATTATGACG+CGG - chr7_3:77134283-77134302 Msa1125680:intron 40.0%
TACCGCGTCATAATTAACAG+TGG + chr7_3:77134288-77134307 None:intergenic 40.0%
! TTTTATTGTCTGAGCGACAG+AGG - chr7_3:77134353-77134372 Msa1125680:intron 40.0%
GAACTCTCTGCTAAGCATTT+GGG + chr7_3:77134437-77134456 None:intergenic 40.0%
AGAACTCTCTGCTAAGCATT+TGG + chr7_3:77134438-77134457 None:intergenic 40.0%
AGAGTTCTACTGCCAAAAAC+TGG - chr7_3:77134450-77134469 Msa1125680:intron 40.0%
GACACTAATGGAATGGAAAG+AGG - chr7_3:77134565-77134584 Msa1125680:intron 40.0%
AAAGAGGTGAGAACACTAGA+TGG - chr7_3:77134581-77134600 Msa1125680:intron 40.0%
TTCCCTAAAGTCCGTTTCTA+TGG - chr7_3:77134680-77134699 Msa1125680:CDS 40.0%
! CAGCCAATAAAGTCTTTTGG+TGG + chr7_3:77134809-77134828 None:intergenic 40.0%
CACCAAAAGACTTTATTGGC+TGG - chr7_3:77134807-77134826 Msa1125680:CDS 40.0%
AGTAGCATCTCCAATGTTGT+TGG - chr7_3:77135140-77135159 Msa1125680:CDS 40.0%
GTACTCAGTACCAACAACAT+TGG + chr7_3:77135153-77135172 None:intergenic 40.0%
TTCTTTAGTCTGTGCTGAAC+TGG - chr7_3:77135630-77135649 Msa1125680:CDS 40.0%
TGCCTGTTTGATCAAATGGT+TGG - chr7_3:77135696-77135715 Msa1125680:intron 40.0%
CCAAATGCTTCACTCAAACA+TGG + chr7_3:77135929-77135948 None:intergenic 40.0%
! CCATGTTTGAGTGAAGCATT+TGG - chr7_3:77135926-77135945 Msa1125680:intron 40.0%
AGAATCACAAAGAACTCGAG+AGG + chr7_3:77135958-77135977 None:intergenic 40.0%
CTCTAAACCTGTGACATCAA+AGG + chr7_3:77135997-77136016 None:intergenic 40.0%
! TTTCTCCTGCAATGCTTTTG+TGG - chr7_3:77136156-77136175 Msa1125680:intron 40.0%
!! ATGTTCCACAAAAGCATTGC+AGG + chr7_3:77136164-77136183 None:intergenic 40.0%
ACATTCTTCGCATGATGTCG+GGG - chr7_3:77133179-77133198 Msa1125680:three_prime_UTR 45.0%
GGAATTGTTCTTGTCTCGGT+TGG - chr7_3:77133821-77133840 Msa1125680:intron 45.0%
TTCTTGTCTCGGTTGGATGT+TGG - chr7_3:77133828-77133847 Msa1125680:intron 45.0%
TCTTGTCTCGGTTGGATGTT+GGG - chr7_3:77133829-77133848 Msa1125680:intron 45.0%
CTTCAGCTGTTTGCAAGAGA+TGG - chr7_3:77133865-77133884 Msa1125680:intron 45.0%
GTCGACTACACGTAAAGAAG+TGG + chr7_3:77133901-77133920 None:intergenic 45.0%
AATAGGTCTTACGCTGCACA+GGG + chr7_3:77133980-77133999 None:intergenic 45.0%
ATCCAACTCTGGCAACTCAA+GGG + chr7_3:77134003-77134022 None:intergenic 45.0%
TTGGTTATTGCTAAACCGGC+AGG - chr7_3:77134052-77134071 Msa1125680:intron 45.0%
CGTGATCAGATCCCTAGTAA+AGG + chr7_3:77134123-77134142 None:intergenic 45.0%
TGATGTTGGAGCACAAGAGA+AGG + chr7_3:77134180-77134199 None:intergenic 45.0%
TCTTGTGCTCCAACATCAAC+CGG - chr7_3:77134182-77134201 Msa1125680:intron 45.0%
GCTATAAGCACGTGTTATCC+TGG - chr7_3:77134248-77134267 Msa1125680:intron 45.0%
TTGACCCACTTGAACGTACT+TGG - chr7_3:77134390-77134409 Msa1125680:intron 45.0%
CAGTCCAAGTACGTTCAAGT+GGG + chr7_3:77134397-77134416 None:intergenic 45.0%
ACAGTCCAAGTACGTTCAAG+TGG + chr7_3:77134398-77134417 None:intergenic 45.0%
TACTGCCAAAAACTGGTCGA+AGG - chr7_3:77134457-77134476 Msa1125680:intron 45.0%
ACTGCCAAAAACTGGTCGAA+GGG - chr7_3:77134458-77134477 Msa1125680:intron 45.0%
! TTTCCCCTTCGACCAGTTTT+TGG + chr7_3:77134465-77134484 None:intergenic 45.0%
!! CATTCTAGGACTTACGTCAC+TGG - chr7_3:77134635-77134654 Msa1125680:CDS 45.0%
GCTCACTGGGATGATATCTA+CGG + chr7_3:77134736-77134755 None:intergenic 45.0%
! GTTTTCATCAACGCCTGCAA+GGG + chr7_3:77135558-77135577 None:intergenic 45.0%
TTCCAAACTATCGAGGATGG+AGG + chr7_3:77135588-77135607 None:intergenic 45.0%
ATGTGGTCCTTTGATGTCAC+AGG - chr7_3:77135987-77136006 Msa1125680:intron 45.0%
!!! TAATATTTTTTATAAAAAGT+TGG - chr7_3:77135476-77135495 Msa1125680:CDS 5.0%
!!! AATATTTTTTATAAAAAGTT+GGG - chr7_3:77135477-77135496 Msa1125680:CDS 5.0%
TTCGCATGATGTCGGGGAAA+AGG - chr7_3:77133185-77133204 Msa1125680:three_prime_UTR 50.0%
CAATAGAAGAGCTGCTGCTG+AGG - chr7_3:77133743-77133762 Msa1125680:intron 50.0%
CAAGACTTCAGCAGGGAGAT+CGG + chr7_3:77133802-77133821 None:intergenic 50.0%
CTTGTCTCGGTTGGATGTTG+GGG - chr7_3:77133830-77133849 Msa1125680:intron 50.0%
GTGTAGTCGACCAATCACCT+TGG - chr7_3:77133910-77133929 Msa1125680:intron 50.0%
GAATAGGTCTTACGCTGCAC+AGG + chr7_3:77133981-77134000 None:intergenic 50.0%
CTCTGGCAACTCAAGGGAAT+AGG + chr7_3:77133997-77134016 None:intergenic 50.0%
! TTCCCTTGAGTTGCCAGAGT+TGG - chr7_3:77133998-77134017 Msa1125680:intron 50.0%
CATCCAACTCTGGCAACTCA+AGG + chr7_3:77134004-77134023 None:intergenic 50.0%
! CTTGAGTTGCCAGAGTTGGA+TGG - chr7_3:77134002-77134021 Msa1125680:intron 50.0%
ACACGACATCCATCCAACTC+TGG + chr7_3:77134014-77134033 None:intergenic 50.0%
TATTGCTAAACCGGCAGGAC+TGG - chr7_3:77134057-77134076 Msa1125680:intron 50.0%
TCACGCTGCCAAAGTTTGAC+AGG - chr7_3:77134138-77134157 Msa1125680:intron 50.0%
! GTGGTCCATTCTTTTTCCCC+AGG + chr7_3:77134269-77134288 None:intergenic 50.0%
ATTGTCTGAGCGACAGAGGT+TGG - chr7_3:77134357-77134376 Msa1125680:intron 50.0%
CTCTCTGCTAAGCATTTGGG+CGG + chr7_3:77134434-77134453 None:intergenic 50.0%
CTGCCAAAAACTGGTCGAAG+GGG - chr7_3:77134459-77134478 Msa1125680:intron 50.0%
! GGTTTTCATCAACGCCTGCA+AGG + chr7_3:77135559-77135578 None:intergenic 50.0%
! TATCGAGGATGGAGGTGTGT+GGG + chr7_3:77135580-77135599 None:intergenic 50.0%
CACCTCCATCCTCGATAGTT+TGG - chr7_3:77135583-77135602 Msa1125680:CDS 50.0%
TAGTTCCTGCACGACAAGCA+GGG + chr7_3:77135878-77135897 None:intergenic 50.0%
CGATCTCCCTGCTGAAGTCT+TGG - chr7_3:77133800-77133819 Msa1125680:intron 55.0%
! TGAGCGACAGAGGTTGGCTA+GGG - chr7_3:77134363-77134382 Msa1125680:intron 55.0%
TCTGCTAAGCATTTGGGCGG+AGG + chr7_3:77134431-77134450 None:intergenic 55.0%
CTGTCACGACACTGCTCACT+GGG + chr7_3:77134749-77134768 None:intergenic 55.0%
CAGTGAGCAGTGTCGTGACA+GGG - chr7_3:77134748-77134767 Msa1125680:CDS 55.0%
! CTATCGAGGATGGAGGTGTG+TGG + chr7_3:77135581-77135600 None:intergenic 55.0%
CTAGTTCCTGCACGACAAGC+AGG + chr7_3:77135879-77135898 None:intergenic 55.0%
CTGCTTGTCGTGCAGGAACT+AGG - chr7_3:77135877-77135896 Msa1125680:intron 55.0%
TGCTAAACCGGCAGGACTGG+CGG - chr7_3:77134060-77134079 Msa1125680:intron 60.0%
! CTGAGCGACAGAGGTTGGCT+AGG - chr7_3:77134362-77134381 Msa1125680:intron 60.0%
! GAGCGACAGAGGTTGGCTAG+GGG - chr7_3:77134364-77134383 Msa1125680:intron 60.0%
CCTGTCACGACACTGCTCAC+TGG + chr7_3:77134750-77134769 None:intergenic 60.0%
CCAGTGAGCAGTGTCGTGAC+AGG - chr7_3:77134747-77134766 Msa1125680:CDS 60.0%
GACTTCCCTGCTTGTCGTGC+AGG - chr7_3:77135870-77135889 Msa1125680:intron 60.0%
! TAAACCGGCAGGACTGGCGG+AGG - chr7_3:77134063-77134082 Msa1125680:intron 65.0%
! CAGGACTGGCGGAGGTTGGA+CGG - chr7_3:77134071-77134090 Msa1125680:intron 65.0%
CCAACCTCCGCCAGTCCTGC+CGG + chr7_3:77134070-77134089 None:intergenic 70.0%
! CCGGCAGGACTGGCGGAGGT+TGG - chr7_3:77134067-77134086 Msa1125680:intron 75.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 77133157 77136291 77133157 ID=Msa1125680;Name=Msa1125680
chr7_3 mRNA 77133157 77136291 77133157 ID=Msa1125680-mRNA-1;Parent=Msa1125680;Name=Msa1125680-mRNA-1;_AED=0.02;_eAED=0.02;_QI=33|1|1|1|0.5|0.33|3|421|379
chr7_3 exon 77133157 77133556 77133157 ID=Msa1125680-mRNA-1:exon:21211;Parent=Msa1125680-mRNA-1
chr7_3 exon 77134592 77135714 77134592 ID=Msa1125680-mRNA-1:exon:21210;Parent=Msa1125680-mRNA-1
chr7_3 exon 77136221 77136291 77136221 ID=Msa1125680-mRNA-1:exon:21209;Parent=Msa1125680-mRNA-1
chr7_3 five_prime_UTR 77136259 77136291 77136259 ID=Msa1125680-mRNA-1:five_prime_utr;Parent=Msa1125680-mRNA-1
chr7_3 CDS 77136221 77136258 77136221 ID=Msa1125680-mRNA-1:cds;Parent=Msa1125680-mRNA-1
chr7_3 CDS 77134613 77135714 77134613 ID=Msa1125680-mRNA-1:cds;Parent=Msa1125680-mRNA-1
chr7_3 three_prime_UTR 77134592 77134612 77134592 ID=Msa1125680-mRNA-1:three_prime_utr;Parent=Msa1125680-mRNA-1
chr7_3 three_prime_UTR 77133157 77133556 77133157 ID=Msa1125680-mRNA-1:three_prime_utr;Parent=Msa1125680-mRNA-1
Gene Sequence

>Msa1125680

ATGATGTCGGGGAAAAGGAAAAAAAAGTCGAAATCATCAATCAAAGACAATAGAAGAGCTGCTGCTGAGGTAAAAAGCAACAATTTGGAGCTGCAAACATGGGCCGATCTCCCTGCTGAAGTCTTGGAATTGTTCTTGTCTCGGTTGGATGTTGGGGATAATATTCGTGCTTCAGCTGTTTGCAAGAGATGGTGTTCAGTTGCCACTTCTTTACGTGTAGTCGACCAATCACCTTGGCTTATGTATTTCCCAAAAAAGGGTAATTGTTATGATTTCTATGACCCTGTGCAGCGTAAGACCTATTCCCTTGAGTTGCCAGAGTTGGATGGATGTCGTGTTTGCTATACAAAAGATGGTTGGTTATTGCTAAACCGGCAGGACTGGCGGAGGTTGGACGGAAATCACATTTTTTCTCTCTTTAATCCCTTTACTAGGGATCTGATCACGCTGCCAAAGTTTGACAGGACATATCAAATTGCTGCCTTCTCTTGTGCTCCAACATCAACCGGATGTGTTATATTAATTTTCAGACGTGTTGGTTCTAGTCTTGTAGCTATAAGCACGTGTTATCCTGGGGAAAAAGAATGGACCACTGTTAATTATGACGCGGTACTCTCATGTAGCATGTGCGATAAGCTTGTCTTTTCTAATGGGTTATTTTATTGTCTGAGCGACAGAGGTTGGCTAGGGGTGTTTGACCCACTTGAACGTACTTGGACTGTTCTCGAAGTACCTCCGCCCAAATGCTTAGCAGAGAGTTCTACTGCCAAAAACTGGTCGAAGGGGAAATTTATGATAGAGCACAAAGGAAATATATTTGTAGTTTATATATGTTGTGGTGAGGACCCAATAATTTTCAAGTTAGATCTGACACTAATGGAATGGAAAGAGGTGAGAACACTAGATGGAGTAACACTTTTTGCTAGTTTTTTGTCATCTCATTCTAGGACTTACGTCACTGGAATAATGAGAAACGGTGTCTACTTCCCTAAAGTCCGTTTCTATGGAAAACGTTGCATATCATTCTCTCTTGATGACCGTAGATATCATCCCAGTGAGCAGTGTCGTGACAGGGTAGAACCAAATACTTTTGAGAACTTCTGGATTGAACCACCAAAAGACTTTATTGGCTGGATGTAA
Protein sequence

>Msa1125680

MMSGKRKKKSKSSIKDNRRAAAEVKSNNLELQTWADLPAEVLELFLSRLDVGDNIRASAVCKRWCSVATSLRVVDQSPWLMYFPKKGNCYDFYDPVQRKTYSLELPELDGCRVCYTKDGWLLLNRQDWRRLDGNHIFSLFNPFTRDLITLPKFDRTYQIAAFSCAPTSTGCVILIFRRVGSSLVAISTCYPGEKEWTTVNYDAVLSCSMCDKLVFSNGLFYCLSDRGWLGVFDPLERTWTVLEVPPPKCLAESSTAKNWSKGKFMIEHKGNIFVVYICCGEDPIIFKLDLTLMEWKEVRTLDGVTLFASFLSSHSRTYVTGIMRNGVYFPKVRFYGKRCISFSLDDRRYHPSEQCRDRVEPNTFENFWIEPPKDFIGWM*