AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1125660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125660 MtrunA17_Chr7g0266441 96.466 283 8 1 1 283 1 281 0.0 565
Msa1125660 MtrunA17_Chr1g0178491 62.021 287 97 6 2 283 1 280 1.07e-122 355
Msa1125660 MtrunA17_Chr7g0266431 84.375 96 14 1 189 283 2 97 1.40e-53 172
Msa1125660 MtrunA17_Chr7g0266421 86.667 90 12 0 14 103 10 99 1.00e-49 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1125660 AT1G57790.1 44.867 263 132 5 24 283 3 255 2.46e-74 231
Msa1125660 AT3G56470.1 43.529 255 127 4 32 283 27 267 1.19e-63 204
Msa1125660 AT4G00893.1 40.392 255 142 5 33 283 44 292 6.86e-59 192
Msa1125660 AT4G10695.2 43.216 199 97 3 46 238 1 189 2.22e-49 163
Msa1125660 AT4G10695.1 43.216 199 97 3 46 238 1 189 2.22e-49 163
Msa1125660 AT4G10660.1 38.767 227 128 3 62 283 3 223 6.41e-47 159
Msa1125660 AT1G49360.5 37.276 279 149 11 8 275 61 324 2.30e-45 159
Msa1125660 AT1G49360.1 37.276 279 149 11 8 275 84 347 3.20e-45 159
Msa1125660 AT3G18720.1 38.095 252 134 7 36 275 51 292 6.37e-45 156
Msa1125660 AT1G49360.3 39.600 250 127 10 37 275 8 244 8.28e-45 155
Msa1125660 AT1G49360.2 39.600 250 127 10 37 275 8 244 8.28e-45 155
Msa1125660 AT1G49360.4 39.600 250 127 10 37 275 8 244 8.28e-45 155
Msa1125660 AT4G12370.1 37.915 211 117 6 81 283 1 205 1.47e-40 142
Msa1125660 AT4G12382.1 45.455 132 66 2 33 164 9 134 1.12e-36 127
Msa1125660 AT4G12382.2 45.455 132 66 2 33 164 9 134 1.12e-36 127
Msa1125660 AT2G44735.1 41.727 139 75 4 139 273 109 245 1.18e-23 99.4
Msa1125660 AT4G10700.1 47.312 93 43 2 62 154 3 89 6.14e-21 89.7

Find 102 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATCTAACTTGAAAATTA+TGG 0.161853 7_3:-77119336 None:intergenic
GAACCACCAAAAGACTTTAT+TGG 0.163822 7_3:+77119596 Msa1125660:three_prime_UTR
CAGAAGTTCTCAAAAGTATT+TGG 0.184683 7_3:-77119569 None:intergenic
TTCCCTAAAGTCCGTTTCTA+TGG 0.238841 7_3:+77119473 Msa1125660:three_prime_UTR
AGCTAAAATATTTATCTCTT+TGG 0.254305 7_3:+77120889 Msa1125660:three_prime_UTR
CCTATGTACAACCTTCAAAT+AGG 0.266334 7_3:-77120828 None:intergenic
TTTCCATAGAAACGGACTTT+AGG 0.272121 7_3:-77119476 None:intergenic
CTCTCTTTAATCCCTTTACT+AGG 0.326943 7_3:+77118902 Msa1125660:CDS
CAAGTTAGATCTGACACTAA+TGG 0.329403 7_3:+77119346 Msa1125660:three_prime_UTR
ATCCAGCCAATAAAGTCTTT+TGG 0.339525 7_3:-77119602 None:intergenic
AGAACTCTCTGCTAAGCATT+TGG 0.351257 7_3:-77119229 None:intergenic
TTGACCCACTTGAACGTACT+TGG 0.354769 7_3:+77119184 Msa1125660:CDS
TTCCATAGAAACGGACTTTA+GGG 0.361226 7_3:-77119475 None:intergenic
AACATTCTTCGCATGATGTC+GGG 0.375799 7_3:+77117972 Msa1125660:exon
TCTTGTCTCGGTTGGATGTT+GGG 0.383783 7_3:+77118623 Msa1125660:CDS
CCATGTTTGAGTGAAGCATT+TGG 0.387770 7_3:+77120717 Msa1125660:three_prime_UTR
AGATCTAACTTGAAAATTAT+GGG 0.392998 7_3:-77119335 None:intergenic
GCTATAAGCACGTGTTATCC+TGG 0.393896 7_3:+77119042 Msa1125660:CDS
ATGTTCCACAAAAGCATTGC+AGG 0.397891 7_3:-77120952 None:intergenic
GAACTCTCTGCTAAGCATTT+GGG 0.407425 7_3:-77119228 None:intergenic
ATGGTTGGTTATTGCTAAAC+CGG 0.409750 7_3:+77118842 Msa1125660:CDS
GGAATTGTTCTTGTCTCGGT+TGG 0.421431 7_3:+77118615 Msa1125660:CDS
GATTAGCAAAACCTATTTGA+AGG 0.423915 7_3:+77120817 Msa1125660:three_prime_UTR
TTCCCTTGAGTTGCCAGAGT+TGG 0.426018 7_3:+77118792 Msa1125660:CDS
AACAATTCCAAGACTTCAGC+AGG 0.429634 7_3:-77118601 None:intergenic
TTCTTGTCTCGGTTGGATGT+TGG 0.436225 7_3:+77118622 Msa1125660:CDS
TCTTGGAATTGTTCTTGTCT+CGG 0.438217 7_3:+77118611 Msa1125660:CDS
TCTTGTGCTCCAACATCAAC+CGG 0.442241 7_3:+77118976 Msa1125660:CDS
TATTGCTAAACCGGCAGGAC+TGG 0.447316 7_3:+77118851 Msa1125660:CDS
ACAATTTGGAGCTGCAAACA+TGG 0.451046 7_3:+77118569 Msa1125660:CDS
TTCGCATGATGTCGGGGAAA+AGG 0.455236 7_3:+77117979 Msa1125660:exon
CTCTGGCAACTCAAGGGAAT+AGG 0.460842 7_3:-77118788 None:intergenic
CGATCTCCCTGCTGAAGTCT+TGG 0.469551 7_3:+77118594 Msa1125660:CDS
AAAGAGGTGAGAACACTAGA+TGG 0.470190 7_3:+77119374 Msa1125660:three_prime_UTR
GAATAGGTCTTACGCTGCAC+AGG 0.470767 7_3:-77118772 None:intergenic
TCTCTTTAATCCCTTTACTA+GGG 0.471216 7_3:+77118903 Msa1125660:CDS
ATGTGGTCCTTTGATGTCAC+AGG 0.471463 7_3:+77120778 Msa1125660:three_prime_UTR
TGATATGTCCTGTCAAACTT+TGG 0.477303 7_3:-77118940 None:intergenic
GAAAATTAATATAACACATC+CGG 0.478730 7_3:-77118995 None:intergenic
AATACATAAGCCAAGGTGAT+TGG 0.482476 7_3:-77118714 None:intergenic
CTTGAGTTGCCAGAGTTGGA+TGG 0.483726 7_3:+77118796 Msa1125660:CDS
CCTGTCACGACACTGCTCAC+TGG 0.484710 7_3:-77119540 None:intergenic
GTGATCAGATCCCTAGTAAA+GGG 0.491655 7_3:-77118913 None:intergenic
CTGTCACGACACTGCTCACT+GGG 0.495007 7_3:-77119539 None:intergenic
CACCAAAAGACTTTATTGGC+TGG 0.495682 7_3:+77119600 Msa1125660:three_prime_UTR
CTGAGCGACAGAGGTTGGCT+AGG 0.495793 7_3:+77119156 Msa1125660:CDS
TGAGCGACAGAGGTTGGCTA+GGG 0.496901 7_3:+77119157 Msa1125660:CDS
CCAACCTCCGCCAGTCCTGC+CGG 0.497826 7_3:-77118861 None:intergenic
CCAGTGAGCAGTGTCGTGAC+AGG 0.497864 7_3:+77119540 Msa1125660:three_prime_UTR
CGTGTTTGCTATACAAAAGA+TGG 0.502296 7_3:+77118823 Msa1125660:CDS
CTATAAGCACGTGTTATCCT+GGG 0.505414 7_3:+77119043 Msa1125660:CDS
CAGGACTGGCGGAGGTTGGA+CGG 0.511557 7_3:+77118865 Msa1125660:CDS
CTTCAGCTGTTTGCAAGAGA+TGG 0.513153 7_3:+77118659 Msa1125660:CDS
CCTATTTGAAGGTTGTACAT+AGG 0.514646 7_3:+77120828 Msa1125660:three_prime_UTR
CCGGCAGGACTGGCGGAGGT+TGG 0.523905 7_3:+77118861 Msa1125660:CDS
ATTGTCTGAGCGACAGAGGT+TGG 0.524197 7_3:+77119151 Msa1125660:CDS
CAAGACTTCAGCAGGGAGAT+CGG 0.525981 7_3:-77118593 None:intergenic
CATTCTAGGACTTACGTCAC+TGG 0.528125 7_3:+77119428 Msa1125660:three_prime_UTR
TTGGTTATTGCTAAACCGGC+AGG 0.529301 7_3:+77118846 Msa1125660:CDS
CGTGATCAGATCCCTAGTAA+AGG 0.536285 7_3:-77118914 None:intergenic
TCACGCTGCCAAAGTTTGAC+AGG 0.539518 7_3:+77118932 Msa1125660:CDS
CCAAATGCTTCACTCAAACA+TGG 0.541352 7_3:-77120717 None:intergenic
GTCACTGGAATAATGAGAAA+CGG 0.542182 7_3:+77119443 Msa1125660:three_prime_UTR
TAGATCTGACACTAATGGAA+TGG 0.542919 7_3:+77119351 Msa1125660:three_prime_UTR
TCTGCTAAGCATTTGGGCGG+AGG 0.544418 7_3:-77119222 None:intergenic
CAACATTCTTCGCATGATGT+CGG 0.545714 7_3:+77117971 Msa1125660:exon
TTTGCTATACAAAAGATGGT+TGG 0.546454 7_3:+77118827 Msa1125660:CDS
CATCCAACTCTGGCAACTCA+AGG 0.553068 7_3:-77118795 None:intergenic
ATAACACATCCGGTTGATGT+TGG 0.553987 7_3:-77118985 None:intergenic
TTGAAATATTAAGAAATCAA+CGG 0.556920 7_3:-77121044 None:intergenic
TTTGGGAAATACATAAGCCA+AGG 0.561817 7_3:-77118721 None:intergenic
CATAGGAAGTTAATATGTGA+AGG 0.564276 7_3:+77120845 Msa1125660:three_prime_UTR
GACACTAATGGAATGGAAAG+AGG 0.564517 7_3:+77119358 Msa1125660:three_prime_UTR
TGATGTTGGAGCACAAGAGA+AGG 0.568366 7_3:-77118971 None:intergenic
TGCTAAACCGGCAGGACTGG+CGG 0.569550 7_3:+77118854 Msa1125660:CDS
CAATAGAAGAGCTGCTGCTG+AGG 0.574535 7_3:+77118537 Msa1125660:CDS
ACACGACATCCATCCAACTC+TGG 0.576746 7_3:-77118805 None:intergenic
CTTGTCTCGGTTGGATGTTG+GGG 0.581584 7_3:+77118624 Msa1125660:CDS
CTCTAAACCTGTGACATCAA+AGG 0.581595 7_3:-77120785 None:intergenic
AGGAATGAATATCTGTGAGT+AGG 0.583262 7_3:+77120865 Msa1125660:three_prime_UTR
GTGATTCTTCATGCAAAATG+TGG 0.585218 7_3:+77120761 Msa1125660:three_prime_UTR
ATCCAACTCTGGCAACTCAA+GGG 0.590798 7_3:-77118794 None:intergenic
TAAACCGGCAGGACTGGCGG+AGG 0.593956 7_3:+77118857 Msa1125660:CDS
ACAGTCCAAGTACGTTCAAG+TGG 0.603382 7_3:-77119189 None:intergenic
CAATTTGGAGCTGCAAACAT+GGG 0.606485 7_3:+77118570 Msa1125660:CDS
GCTCACTGGGATGATATCTA+CGG 0.610400 7_3:-77119526 None:intergenic
GTCGACTACACGTAAAGAAG+TGG 0.612484 7_3:-77118692 None:intergenic
ACATTCTTCGCATGATGTCG+GGG 0.620719 7_3:+77117973 Msa1125660:exon
CAGTGAGCAGTGTCGTGACA+GGG 0.621223 7_3:+77119541 Msa1125660:three_prime_UTR
AATAGGTCTTACGCTGCACA+GGG 0.638547 7_3:-77118771 None:intergenic
CTCTCTGCTAAGCATTTGGG+CGG 0.648219 7_3:-77119225 None:intergenic
TTTGTAGTTTATATATGTTG+TGG 0.650518 7_3:+77119306 Msa1125660:CDS
AGTTTATATATGTTGTGGTG+AGG 0.651124 7_3:+77119311 Msa1125660:CDS
AGAATCACAAAGAACTCGAG+AGG 0.661545 7_3:-77120746 None:intergenic
GAGCGACAGAGGTTGGCTAG+GGG 0.671202 7_3:+77119158 Msa1125660:CDS
CAGTCCAAGTACGTTCAAGT+GGG 0.682692 7_3:-77119188 None:intergenic
GACCACTGTTAATTATGACG+CGG 0.704466 7_3:+77119077 Msa1125660:CDS
TATAAGCACGTGTTATCCTG+GGG 0.707016 7_3:+77119044 Msa1125660:CDS
ACAATTCCAAGACTTCAGCA+GGG 0.713188 7_3:-77118600 None:intergenic
GTGTAGTCGACCAATCACCT+TGG 0.718766 7_3:+77118704 Msa1125660:CDS
TACCGCGTCATAATTAACAG+TGG 0.732690 7_3:-77119079 None:intergenic
AAATTTATGATAGAGCACAA+AGG 0.741593 7_3:+77119276 Msa1125660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATCTTATTTTCAAATA+AGG + chr7_3:77120531-77120550 Msa1125660:intron 10.0%
!!! ATATTTCAATTATATTTTTG+TGG + chr7_3:77121058-77121077 Msa1125660:three_prime_UTR 10.0%
!! CAATAATAATAATATGTATG+GGG - chr7_3:77118056-77118075 None:intergenic 15.0%
!! GCAATAATAATAATATGTAT+GGG - chr7_3:77118057-77118076 None:intergenic 15.0%
!! AGCAATAATAATAATATGTA+TGG - chr7_3:77118058-77118077 None:intergenic 15.0%
!! TACATATTATTATTATTGCT+AGG + chr7_3:77118058-77118077 Msa1125660:intron 15.0%
!! ACATATTATTATTATTGCTA+GGG + chr7_3:77118059-77118078 Msa1125660:intron 15.0%
!!! AATTTTGTTCAACAATTAAT+TGG + chr7_3:77118103-77118122 Msa1125660:intron 15.0%
!! TTTAGATTGATAAACATATT+TGG + chr7_3:77118381-77118400 Msa1125660:intron 15.0%
!! GATGTAAAAAAAAAAATTAG+CGG + chr7_3:77119622-77119641 Msa1125660:three_prime_UTR 15.0%
!! TTGAAATATTAAGAAATCAA+CGG - chr7_3:77121047-77121066 None:intergenic 15.0%
!!! TCAATTTTTTGGCTTAATAA+TGG + chr7_3:77118137-77118156 Msa1125660:intron 20.0%
!! TATGATTTCTATATCCAATT+CGG + chr7_3:77118196-77118215 Msa1125660:intron 20.0%
!! GTTTCATCTATATTTCATAT+TGG + chr7_3:77118232-77118251 Msa1125660:intron 20.0%
!! GAAAATTAATATAACACATC+CGG - chr7_3:77118998-77119017 None:intergenic 20.0%
!! TTTGTAGTTTATATATGTTG+TGG + chr7_3:77119306-77119325 Msa1125660:CDS 20.0%
!! AGATCTAACTTGAAAATTAT+GGG - chr7_3:77119338-77119357 None:intergenic 20.0%
!! AGACTACATATTATAACTAT+TGG + chr7_3:77119705-77119724 Msa1125660:intron 20.0%
!!! ATTTTTCATTTCCAAAACTA+AGG - chr7_3:77120048-77120067 None:intergenic 20.0%
!!! TTAGTTTTGGAAATGAAAAA+TGG + chr7_3:77120047-77120066 Msa1125660:intron 20.0%
!!! TTATTTTCAAATAAGGACTT+GGG + chr7_3:77120538-77120557 Msa1125660:intron 20.0%
!! AGCTAAAATATTTATCTCTT+TGG + chr7_3:77120889-77120908 Msa1125660:three_prime_UTR 20.0%
! ATAATAATATGTATGGGGTT+AGG - chr7_3:77118051-77118070 None:intergenic 25.0%
!!! TTGCACTAACTTCAATTTTT+TGG + chr7_3:77118126-77118145 Msa1125660:intron 25.0%
! CATGAAAAATTTAACCGAAT+TGG - chr7_3:77118213-77118232 None:intergenic 25.0%
! TAGTTAATTCACAATAAAGC+TGG - chr7_3:77118269-77118288 None:intergenic 25.0%
!!! CATTTTTAAGTTGATGATTG+TGG + chr7_3:77118290-77118309 Msa1125660:intron 25.0%
! AGATTGATAAACATATTTGG+TGG + chr7_3:77118384-77118403 Msa1125660:intron 25.0%
! GATTGATAAACATATTTGGT+GGG + chr7_3:77118385-77118404 Msa1125660:intron 25.0%
! CTTATGTATTTCCCAAAAAA+GGG + chr7_3:77118727-77118746 Msa1125660:CDS 25.0%
!!! CATAACAATTACCCTTTTTT+GGG - chr7_3:77118741-77118760 None:intergenic 25.0%
!!! TCATAACAATTACCCTTTTT+TGG - chr7_3:77118742-77118761 None:intergenic 25.0%
!! ATATTAATTTTCAGACGTGT+TGG + chr7_3:77119006-77119025 Msa1125660:CDS 25.0%
!! ATAAGCTTGTCTTTTCTAAT+GGG + chr7_3:77119121-77119140 Msa1125660:CDS 25.0%
! AAATTTATGATAGAGCACAA+AGG + chr7_3:77119276-77119295 Msa1125660:CDS 25.0%
! CAGATCTAACTTGAAAATTA+TGG - chr7_3:77119339-77119358 None:intergenic 25.0%
!!! TTTGTGTAGAGCATTTATTT+TGG + chr7_3:77119670-77119689 Msa1125660:intron 25.0%
!! TATTGGATACATGCTTTATT+AGG + chr7_3:77119722-77119741 Msa1125660:intron 25.0%
! TATTAGGCTGTAAACATTTA+TGG + chr7_3:77119738-77119757 Msa1125660:intron 25.0%
! CAATAAGACTAGGAAAATTT+AGG - chr7_3:77119817-77119836 None:intergenic 25.0%
! TGAGTTATATGCAAATCTTA+AGG + chr7_3:77119884-77119903 Msa1125660:intron 25.0%
!!! CACTAGTTTGTTGTAAAAAT+TGG + chr7_3:77120163-77120182 Msa1125660:intron 25.0%
!! TTTAACTTCAATCGAGATTA+TGG - chr7_3:77120230-77120249 None:intergenic 25.0%
!! AGGGTATTCTATAAAATAAG+AGG - chr7_3:77120331-77120350 None:intergenic 25.0%
! GATAGTTTGGAAAATATAGT+CGG + chr7_3:77120388-77120407 Msa1125660:intron 25.0%
!!! CTTATTTTCAAATAAGGACT+TGG + chr7_3:77120537-77120556 Msa1125660:intron 25.0%
! AGAAAAAGATATGATCAAAC+AGG - chr7_3:77120625-77120644 None:intergenic 25.0%
TTGTTGAACAAAATTGAGAG+TGG - chr7_3:77118098-77118117 None:intergenic 30.0%
AAAAAATGAAGTTAGCTAGC+AGG - chr7_3:77118452-77118471 None:intergenic 30.0%
TGAGGTAAAAAGCAACAATT+TGG + chr7_3:77118555-77118574 Msa1125660:CDS 30.0%
GCTTATGTATTTCCCAAAAA+AGG + chr7_3:77118726-77118745 Msa1125660:CDS 30.0%
! TTTGCTATACAAAAGATGGT+TGG + chr7_3:77118827-77118846 Msa1125660:CDS 30.0%
TCTCTTTAATCCCTTTACTA+GGG + chr7_3:77118903-77118922 Msa1125660:CDS 30.0%
! GATAAGCTTGTCTTTTCTAA+TGG + chr7_3:77119120-77119139 Msa1125660:CDS 30.0%
AGTTTATATATGTTGTGGTG+AGG + chr7_3:77119311-77119330 Msa1125660:CDS 30.0%
!!! GTTTTTTGTCATCTCATTCT+AGG + chr7_3:77119414-77119433 Msa1125660:three_prime_UTR 30.0%
!! CAGAAGTTCTCAAAAGTATT+TGG - chr7_3:77119572-77119591 None:intergenic 30.0%
!! CAAATACTTTTGAGAACTTC+TGG + chr7_3:77119570-77119589 Msa1125660:three_prime_UTR 30.0%
TTGTAGCTAACAATAAGACT+AGG - chr7_3:77119827-77119846 None:intergenic 30.0%
AAATCTTAAGGTTTCTCCAA+TGG + chr7_3:77119896-77119915 Msa1125660:intron 30.0%
! ATGTTCATGATCCTTAGTTT+TGG + chr7_3:77120034-77120053 Msa1125660:intron 30.0%
! ATTTTATAGAATACCCTTGC+AGG + chr7_3:77120334-77120353 Msa1125660:intron 30.0%
! CTATATTTTCCAAACTATCG+AGG - chr7_3:77120387-77120406 None:intergenic 30.0%
ACTGGTATATGATTGCTAAA+TGG + chr7_3:77120440-77120459 Msa1125660:intron 30.0%
! ATTTTGCCTGTTTGATCAAA+TGG + chr7_3:77120484-77120503 Msa1125660:intron 30.0%
GATTAGCAAAACCTATTTGA+AGG + chr7_3:77120817-77120836 Msa1125660:three_prime_UTR 30.0%
CATAGGAAGTTAATATGTGA+AGG + chr7_3:77120845-77120864 Msa1125660:three_prime_UTR 30.0%
GGAGAAAAATCTTGAAATTC+AGG - chr7_3:77120934-77120953 None:intergenic 30.0%
ATATGTATGGGGTTAGGTTA+GGG - chr7_3:77118045-77118064 None:intergenic 35.0%
AATATGTATGGGGTTAGGTT+AGG - chr7_3:77118046-77118065 None:intergenic 35.0%
ATTCACAATAAAGCTGGTCA+TGG - chr7_3:77118263-77118282 None:intergenic 35.0%
GTAAACAGAACAGAAACAGT+GGG - chr7_3:77118346-77118365 None:intergenic 35.0%
AGTAAACAGAACAGAAACAG+TGG - chr7_3:77118347-77118366 None:intergenic 35.0%
! GATAAACATATTTGGTGGGA+TGG + chr7_3:77118389-77118408 Msa1125660:intron 35.0%
TCTTGGAATTGTTCTTGTCT+CGG + chr7_3:77118611-77118630 Msa1125660:CDS 35.0%
AATACATAAGCCAAGGTGAT+TGG - chr7_3:77118717-77118736 None:intergenic 35.0%
TTTGGGAAATACATAAGCCA+AGG - chr7_3:77118724-77118743 None:intergenic 35.0%
!! CGTGTTTGCTATACAAAAGA+TGG + chr7_3:77118823-77118842 Msa1125660:CDS 35.0%
! ATGGTTGGTTATTGCTAAAC+CGG + chr7_3:77118842-77118861 Msa1125660:CDS 35.0%
CTCTCTTTAATCCCTTTACT+AGG + chr7_3:77118902-77118921 Msa1125660:CDS 35.0%
TGATATGTCCTGTCAAACTT+TGG - chr7_3:77118943-77118962 None:intergenic 35.0%
CAAGTTAGATCTGACACTAA+TGG + chr7_3:77119346-77119365 Msa1125660:three_prime_UTR 35.0%
TAGATCTGACACTAATGGAA+TGG + chr7_3:77119351-77119370 Msa1125660:three_prime_UTR 35.0%
GTCACTGGAATAATGAGAAA+CGG + chr7_3:77119443-77119462 Msa1125660:three_prime_UTR 35.0%
! TTCCATAGAAACGGACTTTA+GGG - chr7_3:77119478-77119497 None:intergenic 35.0%
! TTTCCATAGAAACGGACTTT+AGG - chr7_3:77119479-77119498 None:intergenic 35.0%
! TGCAACGTTTTCCATAGAAA+CGG - chr7_3:77119487-77119506 None:intergenic 35.0%
GAACCACCAAAAGACTTTAT+TGG + chr7_3:77119596-77119615 Msa1125660:three_prime_UTR 35.0%
! ATCCAGCCAATAAAGTCTTT+TGG - chr7_3:77119605-77119624 None:intergenic 35.0%
GTAAACATTTATGGAGCTAC+TGG + chr7_3:77119747-77119766 Msa1125660:intron 35.0%
GAATAAGATTGCATGCATCA+TGG - chr7_3:77119860-77119879 None:intergenic 35.0%
ACTAATGCACATGTAACCAT+TGG - chr7_3:77119915-77119934 None:intergenic 35.0%
! GACAGAGTACAATTTTGCTA+TGG + chr7_3:77119983-77120002 Msa1125660:intron 35.0%
TGAAAAATGGTCAGAGTTAG+AGG + chr7_3:77120060-77120079 Msa1125660:intron 35.0%
ACACACATGACTTTCTATGA+TGG - chr7_3:77120197-77120216 None:intergenic 35.0%
! ATTTTCCAAACTATCGAGGA+TGG - chr7_3:77120383-77120402 None:intergenic 35.0%
ATCCAACCATTTGATCAAAC+AGG - chr7_3:77120493-77120512 None:intergenic 35.0%
GTGATTCTTCATGCAAAATG+TGG + chr7_3:77120761-77120780 Msa1125660:three_prime_UTR 35.0%
CCTATGTACAACCTTCAAAT+AGG - chr7_3:77120831-77120850 None:intergenic 35.0%
CCTATTTGAAGGTTGTACAT+AGG + chr7_3:77120828-77120847 Msa1125660:three_prime_UTR 35.0%
AGGAATGAATATCTGTGAGT+AGG + chr7_3:77120865-77120884 Msa1125660:three_prime_UTR 35.0%
CAACATTCTTCGCATGATGT+CGG + chr7_3:77117971-77117990 Msa1125660:exon 40.0%
AACATTCTTCGCATGATGTC+GGG + chr7_3:77117972-77117991 Msa1125660:exon 40.0%
! ACAATTTGGAGCTGCAAACA+TGG + chr7_3:77118569-77118588 Msa1125660:CDS 40.0%
! CAATTTGGAGCTGCAAACAT+GGG + chr7_3:77118570-77118589 Msa1125660:CDS 40.0%
ACAATTCCAAGACTTCAGCA+GGG - chr7_3:77118603-77118622 None:intergenic 40.0%
AACAATTCCAAGACTTCAGC+AGG - chr7_3:77118604-77118623 None:intergenic 40.0%
GTGATCAGATCCCTAGTAAA+GGG - chr7_3:77118916-77118935 None:intergenic 40.0%
! ATAACACATCCGGTTGATGT+TGG - chr7_3:77118988-77119007 None:intergenic 40.0%
CTATAAGCACGTGTTATCCT+GGG + chr7_3:77119043-77119062 Msa1125660:CDS 40.0%
TATAAGCACGTGTTATCCTG+GGG + chr7_3:77119044-77119063 Msa1125660:CDS 40.0%
GTTATCCTGGGGAAAAAGAA+TGG + chr7_3:77119055-77119074 Msa1125660:CDS 40.0%
GACCACTGTTAATTATGACG+CGG + chr7_3:77119077-77119096 Msa1125660:CDS 40.0%
TACCGCGTCATAATTAACAG+TGG - chr7_3:77119082-77119101 None:intergenic 40.0%
! TTTTATTGTCTGAGCGACAG+AGG + chr7_3:77119147-77119166 Msa1125660:CDS 40.0%
GAACTCTCTGCTAAGCATTT+GGG - chr7_3:77119231-77119250 None:intergenic 40.0%
AGAACTCTCTGCTAAGCATT+TGG - chr7_3:77119232-77119251 None:intergenic 40.0%
AGAGTTCTACTGCCAAAAAC+TGG + chr7_3:77119244-77119263 Msa1125660:CDS 40.0%
GACACTAATGGAATGGAAAG+AGG + chr7_3:77119358-77119377 Msa1125660:three_prime_UTR 40.0%
AAAGAGGTGAGAACACTAGA+TGG + chr7_3:77119374-77119393 Msa1125660:three_prime_UTR 40.0%
TTCCCTAAAGTCCGTTTCTA+TGG + chr7_3:77119473-77119492 Msa1125660:three_prime_UTR 40.0%
! CAGCCAATAAAGTCTTTTGG+TGG - chr7_3:77119602-77119621 None:intergenic 40.0%
CACCAAAAGACTTTATTGGC+TGG + chr7_3:77119600-77119619 Msa1125660:three_prime_UTR 40.0%
AGTAGCATCTCCAATGTTGT+TGG + chr7_3:77119932-77119951 Msa1125660:intron 40.0%
GTACTCAGTACCAACAACAT+TGG - chr7_3:77119945-77119964 None:intergenic 40.0%
TTCTTTAGTCTGTGCTGAAC+TGG + chr7_3:77120422-77120441 Msa1125660:intron 40.0%
TGCCTGTTTGATCAAATGGT+TGG + chr7_3:77120488-77120507 Msa1125660:intron 40.0%
CCAAATGCTTCACTCAAACA+TGG - chr7_3:77120720-77120739 None:intergenic 40.0%
! CCATGTTTGAGTGAAGCATT+TGG + chr7_3:77120717-77120736 Msa1125660:three_prime_UTR 40.0%
AGAATCACAAAGAACTCGAG+AGG - chr7_3:77120749-77120768 None:intergenic 40.0%
CTCTAAACCTGTGACATCAA+AGG - chr7_3:77120788-77120807 None:intergenic 40.0%
! TTTCTCCTGCAATGCTTTTG+TGG + chr7_3:77120947-77120966 Msa1125660:three_prime_UTR 40.0%
!! ATGTTCCACAAAAGCATTGC+AGG - chr7_3:77120955-77120974 None:intergenic 40.0%
ACATTCTTCGCATGATGTCG+GGG + chr7_3:77117973-77117992 Msa1125660:exon 45.0%
GGAATTGTTCTTGTCTCGGT+TGG + chr7_3:77118615-77118634 Msa1125660:CDS 45.0%
TTCTTGTCTCGGTTGGATGT+TGG + chr7_3:77118622-77118641 Msa1125660:CDS 45.0%
TCTTGTCTCGGTTGGATGTT+GGG + chr7_3:77118623-77118642 Msa1125660:CDS 45.0%
CTTCAGCTGTTTGCAAGAGA+TGG + chr7_3:77118659-77118678 Msa1125660:CDS 45.0%
GTCGACTACACGTAAAGAAG+TGG - chr7_3:77118695-77118714 None:intergenic 45.0%
AATAGGTCTTACGCTGCACA+GGG - chr7_3:77118774-77118793 None:intergenic 45.0%
ATCCAACTCTGGCAACTCAA+GGG - chr7_3:77118797-77118816 None:intergenic 45.0%
TTGGTTATTGCTAAACCGGC+AGG + chr7_3:77118846-77118865 Msa1125660:CDS 45.0%
CGTGATCAGATCCCTAGTAA+AGG - chr7_3:77118917-77118936 None:intergenic 45.0%
TGATGTTGGAGCACAAGAGA+AGG - chr7_3:77118974-77118993 None:intergenic 45.0%
TCTTGTGCTCCAACATCAAC+CGG + chr7_3:77118976-77118995 Msa1125660:CDS 45.0%
GCTATAAGCACGTGTTATCC+TGG + chr7_3:77119042-77119061 Msa1125660:CDS 45.0%
TTGACCCACTTGAACGTACT+TGG + chr7_3:77119184-77119203 Msa1125660:CDS 45.0%
CAGTCCAAGTACGTTCAAGT+GGG - chr7_3:77119191-77119210 None:intergenic 45.0%
ACAGTCCAAGTACGTTCAAG+TGG - chr7_3:77119192-77119211 None:intergenic 45.0%
TACTGCCAAAAACTGGTCGA+AGG + chr7_3:77119251-77119270 Msa1125660:CDS 45.0%
ACTGCCAAAAACTGGTCGAA+GGG + chr7_3:77119252-77119271 Msa1125660:CDS 45.0%
! TTTCCCCTTCGACCAGTTTT+TGG - chr7_3:77119259-77119278 None:intergenic 45.0%
!! CATTCTAGGACTTACGTCAC+TGG + chr7_3:77119428-77119447 Msa1125660:three_prime_UTR 45.0%
GCTCACTGGGATGATATCTA+CGG - chr7_3:77119529-77119548 None:intergenic 45.0%
! GTTTTCATCAACGCCTGCAA+GGG - chr7_3:77120350-77120369 None:intergenic 45.0%
TTCCAAACTATCGAGGATGG+AGG - chr7_3:77120380-77120399 None:intergenic 45.0%
ATGTGGTCCTTTGATGTCAC+AGG + chr7_3:77120778-77120797 Msa1125660:three_prime_UTR 45.0%
!!! TAATATTTTTTATAAAAAGT+TGG + chr7_3:77120268-77120287 Msa1125660:intron 5.0%
!!! AATATTTTTTATAAAAAGTT+GGG + chr7_3:77120269-77120288 Msa1125660:intron 5.0%
TTCGCATGATGTCGGGGAAA+AGG + chr7_3:77117979-77117998 Msa1125660:exon 50.0%
CAATAGAAGAGCTGCTGCTG+AGG + chr7_3:77118537-77118556 Msa1125660:CDS 50.0%
CAAGACTTCAGCAGGGAGAT+CGG - chr7_3:77118596-77118615 None:intergenic 50.0%
CTTGTCTCGGTTGGATGTTG+GGG + chr7_3:77118624-77118643 Msa1125660:CDS 50.0%
GTGTAGTCGACCAATCACCT+TGG + chr7_3:77118704-77118723 Msa1125660:CDS 50.0%
GAATAGGTCTTACGCTGCAC+AGG - chr7_3:77118775-77118794 None:intergenic 50.0%
CTCTGGCAACTCAAGGGAAT+AGG - chr7_3:77118791-77118810 None:intergenic 50.0%
! TTCCCTTGAGTTGCCAGAGT+TGG + chr7_3:77118792-77118811 Msa1125660:CDS 50.0%
CATCCAACTCTGGCAACTCA+AGG - chr7_3:77118798-77118817 None:intergenic 50.0%
! CTTGAGTTGCCAGAGTTGGA+TGG + chr7_3:77118796-77118815 Msa1125660:CDS 50.0%
ACACGACATCCATCCAACTC+TGG - chr7_3:77118808-77118827 None:intergenic 50.0%
TATTGCTAAACCGGCAGGAC+TGG + chr7_3:77118851-77118870 Msa1125660:CDS 50.0%
TCACGCTGCCAAAGTTTGAC+AGG + chr7_3:77118932-77118951 Msa1125660:CDS 50.0%
! GTGGTCCATTCTTTTTCCCC+AGG - chr7_3:77119063-77119082 None:intergenic 50.0%
ATTGTCTGAGCGACAGAGGT+TGG + chr7_3:77119151-77119170 Msa1125660:CDS 50.0%
CTCTCTGCTAAGCATTTGGG+CGG - chr7_3:77119228-77119247 None:intergenic 50.0%
CTGCCAAAAACTGGTCGAAG+GGG + chr7_3:77119253-77119272 Msa1125660:CDS 50.0%
! GGTTTTCATCAACGCCTGCA+AGG - chr7_3:77120351-77120370 None:intergenic 50.0%
! TATCGAGGATGGAGGTGTGT+GGG - chr7_3:77120372-77120391 None:intergenic 50.0%
CACCTCCATCCTCGATAGTT+TGG + chr7_3:77120375-77120394 Msa1125660:intron 50.0%
CGATCTCCCTGCTGAAGTCT+TGG + chr7_3:77118594-77118613 Msa1125660:CDS 55.0%
! TGAGCGACAGAGGTTGGCTA+GGG + chr7_3:77119157-77119176 Msa1125660:CDS 55.0%
TCTGCTAAGCATTTGGGCGG+AGG - chr7_3:77119225-77119244 None:intergenic 55.0%
CTGTCACGACACTGCTCACT+GGG - chr7_3:77119542-77119561 None:intergenic 55.0%
CAGTGAGCAGTGTCGTGACA+GGG + chr7_3:77119541-77119560 Msa1125660:three_prime_UTR 55.0%
! CTATCGAGGATGGAGGTGTG+TGG - chr7_3:77120373-77120392 None:intergenic 55.0%
CTAGTCCTGCACGACAAGCA+GGG - chr7_3:77120670-77120689 None:intergenic 55.0%
TGCTAAACCGGCAGGACTGG+CGG + chr7_3:77118854-77118873 Msa1125660:CDS 60.0%
! CTGAGCGACAGAGGTTGGCT+AGG + chr7_3:77119156-77119175 Msa1125660:CDS 60.0%
! GAGCGACAGAGGTTGGCTAG+GGG + chr7_3:77119158-77119177 Msa1125660:CDS 60.0%
CCTGTCACGACACTGCTCAC+TGG - chr7_3:77119543-77119562 None:intergenic 60.0%
CCAGTGAGCAGTGTCGTGAC+AGG + chr7_3:77119540-77119559 Msa1125660:three_prime_UTR 60.0%
GACTTCCCTGCTTGTCGTGC+AGG + chr7_3:77120662-77120681 Msa1125660:intron 60.0%
CCTAGTCCTGCACGACAAGC+AGG - chr7_3:77120671-77120690 None:intergenic 60.0%
CCTGCTTGTCGTGCAGGACT+AGG + chr7_3:77120668-77120687 Msa1125660:intron 60.0%
! TAAACCGGCAGGACTGGCGG+AGG + chr7_3:77118857-77118876 Msa1125660:CDS 65.0%
! CAGGACTGGCGGAGGTTGGA+CGG + chr7_3:77118865-77118884 Msa1125660:CDS 65.0%
CCAACCTCCGCCAGTCCTGC+CGG - chr7_3:77118864-77118883 None:intergenic 70.0%
! CCGGCAGGACTGGCGGAGGT+TGG + chr7_3:77118861-77118880 Msa1125660:CDS 75.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 77117951 77121082 77117951 ID=Msa1125660;Name=Msa1125660
chr7_3 mRNA 77117951 77121082 77117951 ID=Msa1125660-mRNA-1;Parent=Msa1125660;Name=Msa1125660-mRNA-1;_AED=0.02;_eAED=0.02;_QI=33|1|1|1|0.5|0.33|3|707|283
chr7_3 exon 77117951 77118021 77117951 ID=Msa1125660-mRNA-1:exon:21193;Parent=Msa1125660-mRNA-1
chr7_3 exon 77118528 77119649 77118528 ID=Msa1125660-mRNA-1:exon:21194;Parent=Msa1125660-mRNA-1
chr7_3 exon 77120684 77121082 77120684 ID=Msa1125660-mRNA-1:exon:21195;Parent=Msa1125660-mRNA-1
chr7_3 five_prime_UTR 77117951 77117983 77117951 ID=Msa1125660-mRNA-1:five_prime_utr;Parent=Msa1125660-mRNA-1
chr7_3 CDS 77117984 77118021 77117984 ID=Msa1125660-mRNA-1:cds;Parent=Msa1125660-mRNA-1
chr7_3 CDS 77118528 77119341 77118528 ID=Msa1125660-mRNA-1:cds;Parent=Msa1125660-mRNA-1
chr7_3 three_prime_UTR 77119342 77119649 77119342 ID=Msa1125660-mRNA-1:three_prime_utr;Parent=Msa1125660-mRNA-1
chr7_3 three_prime_UTR 77120684 77121082 77120684 ID=Msa1125660-mRNA-1:three_prime_utr;Parent=Msa1125660-mRNA-1
Gene Sequence

>Msa1125660

ATGATGTCGGGGAAAAGGAAAAAAAAGTCGAAATCATCAATCAAAGACAATAGAAGAGCTGCTGCTGAGGTAAAAAGCAACAATTTGGAGCTGCAAACATGGGCCGATCTCCCTGCTGAAGTCTTGGAATTGTTCTTGTCTCGGTTGGATGTTGGGGATAATATTCGTGCTTCAGCTGTTTGCAAGAGATGGTGTTCAGTTGCCACTTCTTTACGTGTAGTCGACCAATCACCTTGGCTTATGTATTTCCCAAAAAAGGGTAATTGTTATGATTTCTATGACCCTGTGCAGCGTAAGACCTATTCCCTTGAGTTGCCAGAGTTGGATGGATGTCGTGTTTGCTATACAAAAGATGGTTGGTTATTGCTAAACCGGCAGGACTGGCGGAGGTTGGACGGAAATCACATTTTTTCTCTCTTTAATCCCTTTACTAGGGATCTGATCACGCTGCCAAAGTTTGACAGGACATATCAAATTGCTGCCTTCTCTTGTGCTCCAACATCAACCGGATGTGTTATATTAATTTTCAGACGTGTTGGTTCTAGTCTTGTAGCTATAAGCACGTGTTATCCTGGGGAAAAAGAATGGACCACTGTTAATTATGACGCGGTACTCTCATGTAGCATGTGCGATAAGCTTGTCTTTTCTAATGGGTTATTTTATTGTCTGAGCGACAGAGGTTGGCTAGGGGTGTTTGACCCACTTGAACGTACTTGGACTGTTCTCGAAGTACCTCCGCCCAAATGCTTAGCAGAGAGTTCTACTGCCAAAAACTGGTCGAAGGGGAAATTTATGATAGAGCACAAAGGAAATATATTTGTAGTTTATATATGTTGTGGTGAGGACCCATAA
Protein sequence

>Msa1125660

MMSGKRKKKSKSSIKDNRRAAAEVKSNNLELQTWADLPAEVLELFLSRLDVGDNIRASAVCKRWCSVATSLRVVDQSPWLMYFPKKGNCYDFYDPVQRKTYSLELPELDGCRVCYTKDGWLLLNRQDWRRLDGNHIFSLFNPFTRDLITLPKFDRTYQIAAFSCAPTSTGCVILIFRRVGSSLVAISTCYPGEKEWTTVNYDAVLSCSMCDKLVFSNGLFYCLSDRGWLGVFDPLERTWTVLEVPPPKCLAESSTAKNWSKGKFMIEHKGNIFVVYICCGEDP*