AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1185170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185170 MtrunA17_Chr8g0350821 97.030 101 3 0 1 101 1 101 5.84e-68 198
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1185170 AT2G45695.1 81.188 101 19 0 1 101 1 101 5.42e-56 169
Msa1185170 AT3G61113.1 82.178 101 16 1 1 101 1 99 6.35e-55 166

Find 49 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCAGCTTACACTTGAATT+TGG 0.145864 8_1:-21883283 Msa1185170:CDS
CCATGAAAGGATTGCTTTCT+TGG 0.174797 8_1:-21882075 Msa1185170:CDS
GTTGACATTGTGAATCTTCT+TGG 0.186587 8_1:+21882224 None:intergenic
CTATTATACTTTATTGCTTT+TGG 0.211707 8_1:-21880741 Msa1185170:three_prime_UTR
TCTCCTTTCATAAACATTTC+AGG 0.228546 8_1:+21882025 None:intergenic
ATTTATCCACTTGCTCATAT+TGG 0.261746 8_1:-21880610 Msa1185170:three_prime_UTR
GGTTCAAACCAACTTGATTA+AGG 0.268614 8_1:-21882053 Msa1185170:CDS
GTTGAATTTGAGCAACAAAA+TGG 0.291940 8_1:-21882201 Msa1185170:CDS
GCATACTTGCTTAGGAATAT+AGG 0.293097 8_1:-21880457 Msa1185170:three_prime_UTR
CATACTTGCTTAGGAATATA+GGG 0.293237 8_1:-21880456 Msa1185170:three_prime_UTR
CCAAGAAAGCAATCCTTTCA+TGG 0.307619 8_1:+21882075 None:intergenic
CATTCAATCGTTTCTCTCTC+TGG 0.329951 8_1:-21883334 Msa1185170:five_prime_UTR
CTGTCATATACATAAGGTTT+TGG 0.343621 8_1:-21880517 Msa1185170:three_prime_UTR
TGCAGCTTACACTTGAATTT+GGG 0.364775 8_1:-21883282 Msa1185170:intron
GTTACTTAAATGTTGGTGTA+AGG 0.388448 8_1:-21880633 Msa1185170:three_prime_UTR
TTGCATGGTGGTTAGCTTGT+AGG 0.388601 8_1:-21880926 Msa1185170:exon
CTGAATCAACTTACATAGTC+TGG 0.399028 8_1:-21880814 Msa1185170:three_prime_UTR
CATGAAAGGATTGCTTTCTT+GGG 0.403154 8_1:-21882074 Msa1185170:CDS
TCCTCGTGAATGATTGTGAC+TGG 0.415552 8_1:-21881012 Msa1185170:CDS
GTGGATTGGCATACTTGCTT+AGG 0.430540 8_1:-21880465 Msa1185170:three_prime_UTR
TCCCCTTGTTACTTAAATGT+TGG 0.450230 8_1:-21880640 Msa1185170:three_prime_UTR
TCATCCTGCTTCAATTCGAT+TGG 0.477179 8_1:+21880551 None:intergenic
AGGCCTCTCCTTAATCAAGT+TGG 0.484011 8_1:+21882045 None:intergenic
TCGAACAGTGAGAGATGAAA+GGG 0.484769 8_1:+21883374 None:intergenic
ATCAAACTGTCATATACATA+AGG 0.485750 8_1:-21880523 Msa1185170:three_prime_UTR
GTCTTTATATCAACCTTGCA+TGG 0.507829 8_1:-21880941 Msa1185170:CDS
GACACCAATCGAATTGAAGC+AGG 0.510235 8_1:-21880555 Msa1185170:three_prime_UTR
GGTGAACGCTGATTACAATC+TGG 0.510354 8_1:-21880848 Msa1185170:three_prime_UTR
AGATTGATATGCAGTAAGTA+AGG 0.522381 8_1:+21880670 None:intergenic
GGTTTGTCTTGCAGTAGACC+TGG 0.526827 8_1:-21881043 Msa1185170:intron
TTCACGAGGACGAGAACTCC+AGG 0.527292 8_1:+21881025 None:intergenic
GATCGTCATTCATTGACTGT+TGG 0.538577 8_1:-21880588 Msa1185170:three_prime_UTR
CACCAACATTTAAGTAACAA+GGG 0.561105 8_1:+21880638 None:intergenic
CTCGAACAGTGAGAGATGAA+AGG 0.562371 8_1:+21883373 None:intergenic
GACGATCCAATATGAGCAAG+TGG 0.563519 8_1:+21880604 None:intergenic
AGGCCTGAAATGTTTATGAA+AGG 0.573190 8_1:-21882028 Msa1185170:CDS
ACACCAACATTTAAGTAACA+AGG 0.573993 8_1:+21880637 None:intergenic
TACAAGCTAACCACCATGCA+AGG 0.592370 8_1:+21880928 None:intergenic
CCTCGTGAATGATTGTGACT+GGG 0.607265 8_1:-21881011 Msa1185170:CDS
ACCAACATTTAAGTAACAAG+GGG 0.608548 8_1:+21880639 None:intergenic
TGGTCAACTGAGTACTACAC+TGG 0.630784 8_1:-21880981 Msa1185170:CDS
GCAACAAAATGGAGAAGACA+AGG 0.642595 8_1:-21882190 Msa1185170:intron
AAACCAACTTGATTAAGGAG+AGG 0.653224 8_1:-21882048 Msa1185170:CDS
CCCAGTCACAATCATTCACG+AGG 0.658925 8_1:+21881011 None:intergenic
TTTATATCAACCTTGCATGG+TGG 0.664106 8_1:-21880938 Msa1185170:CDS
GATTGTGACTGGGAGCTAAG+TGG 0.667534 8_1:-21881001 Msa1185170:CDS
CGAACAGTGAGAGATGAAAG+GGG 0.692787 8_1:+21883375 None:intergenic
ACTGGAAGAGAAAGACGTGG+TGG 0.727010 8_1:-21880963 Msa1185170:CDS
TACACTGGAAGAGAAAGACG+TGG 0.770064 8_1:-21880966 Msa1185170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAACTGAAATAATATAA+AGG + chr8_1:21880604-21880623 None:intergenic 10.0%
!!! AAGTTATTTTTTGTTGTTTT+CGG + chr8_1:21880654-21880673 None:intergenic 15.0%
!! AAAACAACAAAAAATAACTT+AGG - chr8_1:21880654-21880673 Msa1185170:three_prime_UTR 15.0%
!! TAGGAATTAAATTAACAAAT+TGG - chr8_1:21880673-21880692 Msa1185170:three_prime_UTR 15.0%
!! AGCTATTAAAATTAACTAAA+AGG + chr8_1:21880754-21880773 None:intergenic 15.0%
!!! TTAGTTAATTTTAATAGCTT+AGG - chr8_1:21880755-21880774 Msa1185170:three_prime_UTR 15.0%
!!! TAGTTAATTTTAATAGCTTA+GGG - chr8_1:21880756-21880775 Msa1185170:three_prime_UTR 15.0%
!! TATATGTATATTATATGTGT+CGG - chr8_1:21880987-21881006 Msa1185170:CDS 15.0%
!!! TTATACAGTTATTTTAGTTT+TGG - chr8_1:21881409-21881428 Msa1185170:intron 15.0%
!!! TTAGAAATTTAATTTTTGTC+TGG - chr8_1:21881558-21881577 Msa1185170:intron 15.0%
!!! ATTTTTATGTGAGTAATTAA+TGG - chr8_1:21881967-21881986 Msa1185170:intron 15.0%
!!! ACCATATTAGTTTTTTATAT+TGG - chr8_1:21882206-21882225 Msa1185170:CDS 15.0%
!!! CCATATTAGTTTTTTATATT+GGG - chr8_1:21882207-21882226 Msa1185170:CDS 15.0%
!! ATATTACTTCATCAAAAATT+TGG + chr8_1:21882960-21882979 None:intergenic 15.0%
!! AGTATAATAAAAACAATTGT+TGG - chr8_1:21882980-21882999 Msa1185170:intron 15.0%
!!! TGATGTATTTTTCATAGTTT+AGG - chr8_1:21880726-21880745 Msa1185170:three_prime_UTR 20.0%
!! ATTAAGTTCATTGATATAAC+TGG - chr8_1:21880822-21880841 Msa1185170:three_prime_UTR 20.0%
!! AATAGTAATTCCAACAATAT+TGG + chr8_1:21881380-21881399 None:intergenic 20.0%
!!! TTTTTCCACTTTATCTTATT+CGG - chr8_1:21881433-21881452 Msa1185170:intron 20.0%
!!! AAATTTAATTTTTGTCTGGT+TGG - chr8_1:21881562-21881581 Msa1185170:intron 20.0%
!!! AATTTAATTTTTGTCTGGTT+GGG - chr8_1:21881563-21881582 Msa1185170:intron 20.0%
!! CATAAAAATAAGCTCATTTA+CGG + chr8_1:21881956-21881975 None:intergenic 20.0%
!! ACTAATAATTACCAAGAAAA+AGG + chr8_1:21882100-21882119 None:intergenic 20.0%
!! AAACTAATATGGTATTAATG+AGG + chr8_1:21882199-21882218 None:intergenic 20.0%
!! CCCAATATAAAAAACTAATA+TGG + chr8_1:21882210-21882229 None:intergenic 20.0%
!! TGTGTAATATTATCATTACA+TGG - chr8_1:21882765-21882784 Msa1185170:intron 20.0%
!!! CTATTATACTTTATTGCTTT+TGG - chr8_1:21883116-21883135 Msa1185170:intron 20.0%
! ATGGTAATAGAACTTAGTTT+AGG + chr8_1:21881337-21881356 None:intergenic 25.0%
! AAACTAAGTTCTATTACCAT+AGG - chr8_1:21881337-21881356 Msa1185170:intron 25.0%
! ATATCTTCATCCAATATTGT+TGG - chr8_1:21881367-21881386 Msa1185170:intron 25.0%
! AAGTCTGATTAATATCCATT+TGG - chr8_1:21881508-21881527 Msa1185170:intron 25.0%
! ATAACTACATCAAAACCAAA+TGG + chr8_1:21881526-21881545 None:intergenic 25.0%
! AATCACTTAAGTTCAGATAA+TGG + chr8_1:21882028-21882047 None:intergenic 25.0%
! ATAGAAAGCTTATCAACTAA+GGG + chr8_1:21882069-21882088 None:intergenic 25.0%
! AATAGAAAGCTTATCAACTA+AGG + chr8_1:21882070-21882089 None:intergenic 25.0%
!! TATTAGATGATCCTTTTTCT+TGG - chr8_1:21882086-21882105 Msa1185170:intron 25.0%
!!! ATCCTTTGATAAGTTGATAT+AGG - chr8_1:21882709-21882728 Msa1185170:intron 25.0%
! ATCCTATATCAACTTATCAA+AGG + chr8_1:21882714-21882733 None:intergenic 25.0%
!! TATTACACATTTTATGCATC+TGG + chr8_1:21882754-21882773 None:intergenic 25.0%
! ATCAAACTGTCATATACATA+AGG - chr8_1:21883334-21883353 Msa1185170:five_prime_UTR 25.0%
!!! AAATTGGTTGTTTTTCACCA+TGG - chr8_1:21880689-21880708 Msa1185170:three_prime_UTR 30.0%
!! TTGTTTTTCACCATGGTTTA+GGG - chr8_1:21880696-21880715 Msa1185170:three_prime_UTR 30.0%
! TATATTATATGTGTCGGTGT+CGG - chr8_1:21880993-21881012 Msa1185170:CDS 30.0%
TTTATCTTATTCGGCTGTTT+AGG - chr8_1:21881442-21881461 Msa1185170:intron 30.0%
GTTGAATTTGAGCAACAAAA+TGG - chr8_1:21881656-21881675 Msa1185170:intron 30.0%
!! AAGGTAGTATATGACTTGTT+TGG - chr8_1:21881686-21881705 Msa1185170:intron 30.0%
!! TATATGACTTGTTTGGTCTA+TGG - chr8_1:21881693-21881712 Msa1185170:intron 30.0%
! ACTTTTCAGTTAACCATGAA+AGG - chr8_1:21881769-21881788 Msa1185170:intron 30.0%
TCTCCTTTCATAAACATTTC+AGG + chr8_1:21881835-21881854 None:intergenic 30.0%
TAACTAATGATGCATGATTC+AGG + chr8_1:21881887-21881906 None:intergenic 30.0%
ACTTAAGATAAAATTGACCG+TGG + chr8_1:21881998-21882017 None:intergenic 30.0%
GAACTTAAGTGATTGAATGA+TGG - chr8_1:21882034-21882053 Msa1185170:CDS 30.0%
AAGGGAGAACTTTAATAGAA+AGG - chr8_1:21882152-21882171 Msa1185170:intron 30.0%
TATTAATGAGGCTCAACTTA+AGG + chr8_1:21882187-21882206 None:intergenic 30.0%
!! CAGTTTTATAAGGATGAGTT+AGG + chr8_1:21882233-21882252 None:intergenic 30.0%
!! CCTTACAAGTCAGTTTTATA+AGG + chr8_1:21882243-21882262 None:intergenic 30.0%
!! CCTTATAAAACTGACTTGTA+AGG - chr8_1:21882240-21882259 Msa1185170:CDS 30.0%
TGACAAGAGTTTATACAGAT+TGG + chr8_1:21882273-21882292 None:intergenic 30.0%
! ATGAATTCAATGAGAGTACA+TGG - chr8_1:21882489-21882508 Msa1185170:intron 30.0%
ACACACAGAAATAAATGGTT+CGG + chr8_1:21882794-21882813 None:intergenic 30.0%
AAACCACACACAGAAATAAA+TGG + chr8_1:21882799-21882818 None:intergenic 30.0%
AAAAACAATTGTTGGAGTCA+TGG - chr8_1:21882988-21883007 Msa1185170:intron 30.0%
AGATTGATATGCAGTAAGTA+AGG + chr8_1:21883190-21883209 None:intergenic 30.0%
ACCAACATTTAAGTAACAAG+GGG + chr8_1:21883221-21883240 None:intergenic 30.0%
CACCAACATTTAAGTAACAA+GGG + chr8_1:21883222-21883241 None:intergenic 30.0%
ACACCAACATTTAAGTAACA+AGG + chr8_1:21883223-21883242 None:intergenic 30.0%
GTTACTTAAATGTTGGTGTA+AGG - chr8_1:21883224-21883243 Msa1185170:intron 30.0%
ATTTATCCACTTGCTCATAT+TGG - chr8_1:21883247-21883266 Msa1185170:intron 30.0%
! CTGTCATATACATAAGGTTT+TGG - chr8_1:21883340-21883359 Msa1185170:five_prime_UTR 30.0%
CAATCCACAAAAAAAATGTC+AGG + chr8_1:21883380-21883399 None:intergenic 30.0%
!!! CTGACATTTTTTTTGTGGAT+TGG - chr8_1:21883378-21883397 Msa1185170:five_prime_UTR 30.0%
CATACTTGCTTAGGAATATA+GGG - chr8_1:21883401-21883420 Msa1185170:five_prime_UTR 30.0%
ATGCAGCTTACACTTGAATT+TGG - chr8_1:21880574-21880593 Msa1185170:three_prime_UTR 35.0%
TGCAGCTTACACTTGAATTT+GGG - chr8_1:21880575-21880594 Msa1185170:three_prime_UTR 35.0%
!!! GTTGTTTTTCACCATGGTTT+AGG - chr8_1:21880695-21880714 Msa1185170:three_prime_UTR 35.0%
TCAATTTAGTCCCTAAACCA+TGG + chr8_1:21880709-21880728 None:intergenic 35.0%
ATTTGAACTGATTGCCTAGT+TGG - chr8_1:21880856-21880875 Msa1185170:three_prime_UTR 35.0%
!! TATTATTGGTTTCAGCGTGT+TGG - chr8_1:21881194-21881213 Msa1185170:intron 35.0%
AACAGCCGAATAAGATAAAG+TGG + chr8_1:21881441-21881460 None:intergenic 35.0%
! TTTTGTCTGGTTGGGTAAAA+TGG - chr8_1:21881571-21881590 Msa1185170:intron 35.0%
AAAATGGTTGAAACATGCAG+AGG - chr8_1:21881587-21881606 Msa1185170:intron 35.0%
AAATGGTTGAAACATGCAGA+GGG - chr8_1:21881588-21881607 Msa1185170:intron 35.0%
GTTGACATTGTGAATCTTCT+TGG + chr8_1:21881636-21881655 None:intergenic 35.0%
CATGAAAGGATTGCTTTCTT+GGG - chr8_1:21881783-21881802 Msa1185170:intron 35.0%
GGTTCAAACCAACTTGATTA+AGG - chr8_1:21881804-21881823 Msa1185170:intron 35.0%
AAACCAACTTGATTAAGGAG+AGG - chr8_1:21881809-21881828 Msa1185170:intron 35.0%
AGGCCTGAAATGTTTATGAA+AGG - chr8_1:21881829-21881848 Msa1185170:intron 35.0%
TAACTAATGATGCATGACTC+AGG + chr8_1:21881923-21881942 None:intergenic 35.0%
AGTAATTAATGGTCTAGCCA+CGG - chr8_1:21881978-21881997 Msa1185170:intron 35.0%
ACCACAGAAAAACCTTTCAT+GGG + chr8_1:21882131-21882150 None:intergenic 35.0%
! AAAGGTTTTTCTGTGGTCAA+GGG - chr8_1:21882134-21882153 Msa1185170:intron 35.0%
! AGAGTCCTATCTTTTCTATG+CGG - chr8_1:21882293-21882312 Msa1185170:intron 35.0%
TCTGTTAGAGACATATGTTC+TGG - chr8_1:21882632-21882651 Msa1185170:intron 35.0%
TGATCAGAATGATCACCATA+AGG + chr8_1:21882667-21882686 None:intergenic 35.0%
!! GAACCATTTATTTCTGTGTG+TGG - chr8_1:21882793-21882812 Msa1185170:intron 35.0%
GTCTTTATATCAACCTTGCA+TGG - chr8_1:21882916-21882935 Msa1185170:intron 35.0%
TTTATATCAACCTTGCATGG+TGG - chr8_1:21882919-21882938 Msa1185170:intron 35.0%
CTGAATCAACTTACATAGTC+TGG - chr8_1:21883043-21883062 Msa1185170:intron 35.0%
! TCCCCTTGTTACTTAAATGT+TGG - chr8_1:21883217-21883236 Msa1185170:intron 35.0%
GCATACTTGCTTAGGAATAT+AGG - chr8_1:21883400-21883419 Msa1185170:five_prime_UTR 35.0%
TCGAACAGTGAGAGATGAAA+GGG + chr8_1:21880486-21880505 None:intergenic 40.0%
CATTCAATCGTTTCTCTCTC+TGG - chr8_1:21880523-21880542 Msa1185170:three_prime_UTR 40.0%
TAGTTTGACCCTATGAAGCA+CGG + chr8_1:21881043-21881062 None:intergenic 40.0%
TATGAAGCACATACACTCCT+CGG - chr8_1:21881076-21881095 Msa1185170:intron 40.0%
TATCTGTGTCAGACACATGT+CGG - chr8_1:21881109-21881128 Msa1185170:intron 40.0%
CAAACAAGACCTATGAAGCA+CGG + chr8_1:21881247-21881266 None:intergenic 40.0%
! GTGTACAGACTTTTGTGTTG+TGG - chr8_1:21881288-21881307 Msa1185170:intron 40.0%
TGAAGATATCAGCATGCCTA+TGG + chr8_1:21881356-21881375 None:intergenic 40.0%
TCTTATTCGGCTGTTTAGGT+CGG - chr8_1:21881446-21881465 Msa1185170:intron 40.0%
AATGGTTGAAACATGCAGAG+GGG - chr8_1:21881589-21881608 Msa1185170:intron 40.0%
GCAACAAAATGGAGAAGACA+AGG - chr8_1:21881667-21881686 Msa1185170:intron 40.0%
CCAAGAAAGCAATCCTTTCA+TGG + chr8_1:21881785-21881804 None:intergenic 40.0%
CCATGAAAGGATTGCTTTCT+TGG - chr8_1:21881782-21881801 Msa1185170:intron 40.0%
CTAATGATGCATGATTCAGG+TGG + chr8_1:21881884-21881903 None:intergenic 40.0%
TAGTCGTTAACACCCATGAA+AGG - chr8_1:21882116-21882135 Msa1185170:intron 40.0%
! ACCCATGAAAGGTTTTTCTG+TGG - chr8_1:21882127-21882146 Msa1185170:intron 40.0%
GACCACAGAAAAACCTTTCA+TGG + chr8_1:21882132-21882151 None:intergenic 40.0%
! GAAAGGTTTTTCTGTGGTCA+AGG - chr8_1:21882133-21882152 Msa1185170:intron 40.0%
!! AAACTGACTTGTAAGGTGAG+TGG - chr8_1:21882247-21882266 Msa1185170:CDS 40.0%
! GAGTCCTATCTTTTCTATGC+GGG - chr8_1:21882294-21882313 Msa1185170:intron 40.0%
AAGTCCCGCATAGAAAAGAT+AGG + chr8_1:21882301-21882320 None:intergenic 40.0%
! TATCTTTTCTATGCGGGACT+TGG - chr8_1:21882300-21882319 Msa1185170:intron 40.0%
! ATCTTTTCTATGCGGGACTT+GGG - chr8_1:21882301-21882320 Msa1185170:intron 40.0%
GTATCAGAGCCTAACATTTC+AGG + chr8_1:21882389-21882408 None:intergenic 40.0%
GTTAACTAGAGGTAGTTACG+AGG + chr8_1:21882543-21882562 None:intergenic 40.0%
!! GACATGTGTTGGTTAACTAG+AGG + chr8_1:21882554-21882573 None:intergenic 40.0%
TGGGAACTGTCAGTCATTTA+GGG + chr8_1:21882587-21882606 None:intergenic 40.0%
TTCTGGTGTGATACACCTTA+TGG - chr8_1:21882649-21882668 Msa1185170:intron 40.0%
! GATCGTCATTCATTGACTGT+TGG - chr8_1:21883269-21883288 Msa1185170:intron 40.0%
TCATCCTGCTTCAATTCGAT+TGG + chr8_1:21883309-21883328 None:intergenic 40.0%
!! AAGGTTTTGGCAACAAGTTC+AGG - chr8_1:21883353-21883372 Msa1185170:five_prime_UTR 40.0%
!! AGGTTTTGGCAACAAGTTCA+GGG - chr8_1:21883354-21883373 Msa1185170:five_prime_UTR 40.0%
!!! AGGGCCTGACATTTTTTTTG+TGG - chr8_1:21883373-21883392 Msa1185170:five_prime_UTR 40.0%
CGAACAGTGAGAGATGAAAG+GGG + chr8_1:21880485-21880504 None:intergenic 45.0%
CTCGAACAGTGAGAGATGAA+AGG + chr8_1:21880487-21880506 None:intergenic 45.0%
! TGATTGCCTAGTTGGTTCAC+CGG - chr8_1:21880864-21880883 Msa1185170:three_prime_UTR 45.0%
AATGAACCGGTGAACCAACT+AGG + chr8_1:21880873-21880892 None:intergenic 45.0%
!! AGTTGGTTCACCGGTTCATT+TGG - chr8_1:21880873-21880892 Msa1185170:three_prime_UTR 45.0%
! CGTAGGTTGACCAAATGAAC+CGG + chr8_1:21880886-21880905 None:intergenic 45.0%
TATGAAGCACGGACACTACT+CGG + chr8_1:21881032-21881051 None:intergenic 45.0%
AGTAGTGTCCGTGCTTCATA+GGG - chr8_1:21881032-21881051 Msa1185170:CDS 45.0%
ACAGATAGACACCTAATCCG+AGG + chr8_1:21881096-21881115 None:intergenic 45.0%
CATATGTGTCTGTGTCGTGT+CGG + chr8_1:21881144-21881163 None:intergenic 45.0%
ATGGTTGAAACATGCAGAGG+GGG - chr8_1:21881590-21881609 Msa1185170:intron 45.0%
AGGCCTCTCCTTAATCAAGT+TGG + chr8_1:21881815-21881834 None:intergenic 45.0%
GCATCATGCCCAATAGTGAT+GGG + chr8_1:21882350-21882369 None:intergenic 45.0%
ATTGGGCATGATGCGTGTAA+TGG - chr8_1:21882356-21882375 Msa1185170:intron 45.0%
GCGTGTTAACTGACATGTGT+TGG + chr8_1:21882565-21882584 None:intergenic 45.0%
CTGGGAACTGTCAGTCATTT+AGG + chr8_1:21882588-21882607 None:intergenic 45.0%
! CCACTCTAACTGACTCATCT+GGG + chr8_1:21882606-21882625 None:intergenic 45.0%
! ACCACTCTAACTGACTCATC+TGG + chr8_1:21882607-21882626 None:intergenic 45.0%
TCCTCGTGAATGATTGTGAC+TGG - chr8_1:21882845-21882864 Msa1185170:intron 45.0%
CCTCGTGAATGATTGTGACT+GGG - chr8_1:21882846-21882865 Msa1185170:intron 45.0%
TGGTCAACTGAGTACTACAC+TGG - chr8_1:21882876-21882895 Msa1185170:intron 45.0%
TACACTGGAAGAGAAAGACG+TGG - chr8_1:21882891-21882910 Msa1185170:intron 45.0%
TACAAGCTAACCACCATGCA+AGG + chr8_1:21882932-21882951 None:intergenic 45.0%
!! TTGCATGGTGGTTAGCTTGT+AGG - chr8_1:21882931-21882950 Msa1185170:intron 45.0%
! GGTGAACGCTGATTACAATC+TGG - chr8_1:21883009-21883028 Msa1185170:intron 45.0%
GACGATCCAATATGAGCAAG+TGG + chr8_1:21883256-21883275 None:intergenic 45.0%
GACACCAATCGAATTGAAGC+AGG - chr8_1:21883302-21883321 Msa1185170:exon 45.0%
!! GTGGATTGGCATACTTGCTT+AGG - chr8_1:21883392-21883411 Msa1185170:five_prime_UTR 45.0%
!!! TCATTTTTTTAAATTATTAT+TGG - chr8_1:21881180-21881199 Msa1185170:intron 5.0%
GAGTAGTGTCCGTGCTTCAT+AGG - chr8_1:21881031-21881050 Msa1185170:CDS 50.0%
GCACATACACTCCTCGGATT+AGG - chr8_1:21881082-21881101 Msa1185170:intron 50.0%
TGTCTGTGTCCGTGCTTCAT+AGG - chr8_1:21881235-21881254 Msa1185170:intron 50.0%
TGAAACATGCAGAGGGGGTT+TGG - chr8_1:21881595-21881614 Msa1185170:intron 50.0%
! ATGGGCATGAGGGGTGTATT+GGG + chr8_1:21882332-21882351 None:intergenic 50.0%
!! CCAATAGTGATGGGCATGAG+GGG + chr8_1:21882341-21882360 None:intergenic 50.0%
!! CCCAATAGTGATGGGCATGA+GGG + chr8_1:21882342-21882361 None:intergenic 50.0%
CCCTCATGCCCATCACTATT+GGG - chr8_1:21882339-21882358 Msa1185170:intron 50.0%
CGCATCATGCCCAATAGTGA+TGG + chr8_1:21882351-21882370 None:intergenic 50.0%
GGCATGATGCGTGTAATGGT+GGG - chr8_1:21882360-21882379 Msa1185170:intron 50.0%
! ATGATGCGTGTAATGGTGGG+TGG - chr8_1:21882363-21882382 Msa1185170:intron 50.0%
CCCAGATGAGTCAGTTAGAG+TGG - chr8_1:21882603-21882622 Msa1185170:intron 50.0%
GGTTTGTCTTGCAGTAGACC+TGG - chr8_1:21882814-21882833 Msa1185170:intron 50.0%
CCCAGTCACAATCATTCACG+AGG + chr8_1:21882849-21882868 None:intergenic 50.0%
GATTGTGACTGGGAGCTAAG+TGG - chr8_1:21882856-21882875 Msa1185170:intron 50.0%
ACTGGAAGAGAAAGACGTGG+TGG - chr8_1:21882894-21882913 Msa1185170:intron 50.0%
TGTCTGTGCTCGTGCTTCGT+AGG + chr8_1:21880903-21880922 None:intergenic 55.0%
ACGAAGCACGAGCACAGACA+TGG - chr8_1:21880903-21880922 Msa1185170:three_prime_UTR 55.0%
CGAAGCACGAGCACAGACAT+GGG - chr8_1:21880904-21880923 Msa1185170:three_prime_UTR 55.0%
TGGGATACGACACGCTGACA+CGG - chr8_1:21880923-21880942 Msa1185170:exon 55.0%
GCACGGACACTACTCGGATT+AGG + chr8_1:21881026-21881045 None:intergenic 55.0%
TGAAGCACGGACACAGACAC+TGG + chr8_1:21881234-21881253 None:intergenic 55.0%
! TGGGCATGAGGGGTGTATTG+GGG + chr8_1:21882331-21882350 None:intergenic 55.0%
! GATGGGCATGAGGGGTGTAT+TGG + chr8_1:21882333-21882352 None:intergenic 55.0%
CCCCTCATGCCCATCACTAT+TGG - chr8_1:21882338-21882357 Msa1185170:intron 55.0%
!! GCCCAATAGTGATGGGCATG+AGG + chr8_1:21882343-21882362 None:intergenic 55.0%
GGGCATGATGCGTGTAATGG+TGG - chr8_1:21882359-21882378 Msa1185170:intron 55.0%
GGTGGGTGGCCTGAAATGTT+AGG - chr8_1:21882377-21882396 Msa1185170:intron 55.0%
TTCACGAGGACGAGAACTCC+AGG + chr8_1:21882835-21882854 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 21880423 21883456 21880423 ID=Msa1185170;Name=Msa1185170
chr8_1 mRNA 21880423 21883456 21880423 ID=Msa1185170-mRNA-1;Parent=Msa1185170;Name=Msa1185170-mRNA-1;_AED=0.00;_eAED=0.00;_QI=151|1|1|1|0.66|0.5|4|511|101
chr8_1 exon 21880423 21881051 21880423 ID=Msa1185170-mRNA-1:exon:8183;Parent=Msa1185170-mRNA-1
chr8_1 exon 21882019 21882101 21882019 ID=Msa1185170-mRNA-1:exon:8182;Parent=Msa1185170-mRNA-1
chr8_1 exon 21882191 21882272 21882191 ID=Msa1185170-mRNA-1:exon:8181;Parent=Msa1185170-mRNA-1
chr8_1 exon 21883283 21883456 21883283 ID=Msa1185170-mRNA-1:exon:8180;Parent=Msa1185170-mRNA-1
chr8_1 five_prime_UTR 21883306 21883456 21883306 ID=Msa1185170-mRNA-1:five_prime_utr;Parent=Msa1185170-mRNA-1
chr8_1 CDS 21883283 21883305 21883283 ID=Msa1185170-mRNA-1:cds;Parent=Msa1185170-mRNA-1
chr8_1 CDS 21882191 21882272 21882191 ID=Msa1185170-mRNA-1:cds;Parent=Msa1185170-mRNA-1
chr8_1 CDS 21882019 21882101 21882019 ID=Msa1185170-mRNA-1:cds;Parent=Msa1185170-mRNA-1
chr8_1 CDS 21880934 21881051 21880934 ID=Msa1185170-mRNA-1:cds;Parent=Msa1185170-mRNA-1
chr8_1 three_prime_UTR 21880423 21880933 21880423 ID=Msa1185170-mRNA-1:three_prime_utr;Parent=Msa1185170-mRNA-1
Gene Sequence

>Msa1185170

ATGCAGCTTACACTTGAATTTGGAGGGGGTTTGGAGCTTCTTTGTGAGTCCAAGAAGATTCACAATGTCAACGTTGAATTTGAGCAACAAAATGGAGAAGACAAGTTAACCATGAAAGGATTGCTTTCTTGGGTTCAAACCAACTTGATTAAGGAGAGGCCTGAAATGTTTATGAAAGGAGATACTGTTAGACCTGGAGTTCTCGTCCTCGTGAATGATTGTGACTGGGAGCTAAGTGGTCAACTGAGTACTACACTGGAAGAGAAAGACGTGGTGGTCTTTATATCAACCTTGCATGGTGGTTAG
Protein sequence

>Msa1185170

MQLTLEFGGGLELLCESKKIHNVNVEFEQQNGEDKLTMKGLLSWVQTNLIKERPEMFMKGDTVRPGVLVLVNDCDWELSGQLSTTLEEKDVVVFISTLHGG*