AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1101580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTCATCGATTGGAAATT+TGG 0.104984 7_3:-32362182 None:intergenic
GAGAAAGGAATTTGGAAAAT+TGG 0.143332 7_3:-32362131 None:intergenic
GAAGTTGTCCCTTCTGGATT+TGG 0.232227 7_3:+32363569 Msa1101580:CDS
AAGTTGTCCCTTCTGGATTT+GGG 0.234508 7_3:+32363570 Msa1101580:CDS
ATAAAGTTTATGCAAATTAA+AGG 0.241830 7_3:-32363866 None:intergenic
GGAGATGAAGTTGTCCCTTC+TGG 0.258232 7_3:+32363563 Msa1101580:CDS
ACGAGAGAGAGAAAGGAATT+TGG 0.263123 7_3:-32362139 None:intergenic
AAGAAACCTTCATTAAATTC+AGG 0.300861 7_3:-32363531 None:intergenic
ACAAAACACTAGTTATATTA+AGG 0.313616 7_3:-32364224 None:intergenic
AATTTATTATAAAATATAAT+AGG 0.332510 7_3:-32364363 None:intergenic
CAAGACTAAGAGTAGAAGAT+AGG 0.335113 7_3:+32364163 Msa1101580:three_prime_UTR
ATCAATGAATGAATCAAAAC+TGG 0.347024 7_3:-32362281 None:intergenic
AAGCAGCATTTCATTGAGTT+AGG 0.355742 7_3:+32363716 Msa1101580:CDS
ACAACTACAGCTACAATTAC+AGG 0.363670 7_3:+32363633 Msa1101580:CDS
CATTGATTTGAAGCTGTATA+AGG 0.365426 7_3:+32362493 Msa1101580:CDS
CTAAAGGCACTTCATTGTTT+AGG 0.389663 7_3:-32363989 None:intergenic
GATTGGAGCTTCATGCTGAT+TGG 0.395968 7_3:-32362235 None:intergenic
TTTGTGTTTGATCAATCATA+TGG 0.408440 7_3:+32364049 Msa1101580:three_prime_UTR
CAGCTGGAAGATCTCCTCTA+TGG 0.416917 7_3:+32363282 Msa1101580:CDS
GTAGTTGTATGTATATCATA+TGG 0.418651 7_3:-32363618 None:intergenic
GTTTGAAACGAGAGAGAGAA+AGG 0.433383 7_3:-32362146 None:intergenic
GATTGGATCTTCAAAACGAT+TGG 0.447277 7_3:-32362218 None:intergenic
ACTGGATCGGAAAACGAGAT+TGG 0.447361 7_3:-32362263 None:intergenic
GTCCCTTCTGGATTTGGGGT+TGG 0.458123 7_3:+32363575 Msa1101580:CDS
AGAAACCTTCATTAAATTCA+GGG 0.460737 7_3:-32363530 None:intergenic
CAGATCGGAAGGTGTTCGAT+TGG 0.469245 7_3:-32362355 None:intergenic
CCAGCTTCATCTTCATCGAT+TGG 0.471637 7_3:-32362190 None:intergenic
AATGTGTGATGCTGCTGTCT+TGG 0.473472 7_3:+32363788 Msa1101580:three_prime_UTR
CGATCTGAAACTACTGGCTC+AGG 0.479030 7_3:+32362371 Msa1101580:five_prime_UTR
AATTTGATATCCAGACAACT+TGG 0.487685 7_3:+32363255 Msa1101580:intron
CAGACAACTTGGACTGCAGC+TGG 0.489066 7_3:+32363266 Msa1101580:intron
AATTCTGGTACTAGAGAGTC+AGG 0.490190 7_3:-32363911 None:intergenic
AGGAGCCCTGAATTTAATGA+AGG 0.493408 7_3:+32363525 Msa1101580:CDS
ATCGATTGACGATGAAGGAT+TGG 0.495620 7_3:-32362321 None:intergenic
TGGAGAAAGAGAGAGAAAGA+TGG 0.495779 7_3:-32362111 None:intergenic
TGTATAAGGAAACAAATCTC+AGG 0.503963 7_3:+32362507 Msa1101580:CDS
CTCTATCCTGTCATTGCACC+CGG 0.507042 7_3:+32363690 Msa1101580:CDS
GACAAGGCTTGAGAAACAAT+TGG 0.508496 7_3:+32364106 Msa1101580:three_prime_UTR
AAGACTAAGAGTAGAAGATA+GGG 0.508621 7_3:+32364164 Msa1101580:three_prime_UTR
AAACGAGATTGGAAAACGAT+TGG 0.517884 7_3:-32362252 None:intergenic
GACGAATCGATTGACGATGA+AGG 0.521751 7_3:-32362326 None:intergenic
GTCTGAAACGAGAAAGAGAA+AGG 0.522376 7_3:-32362082 None:intergenic
TTGGCAAAAGAGTAAAAGAC+GGG 0.531616 7_3:+32364125 Msa1101580:three_prime_UTR
TAAAAGACGGGGCAATTACA+AGG 0.536908 7_3:+32364137 Msa1101580:three_prime_UTR
ATGAAGGTTTCTTCACCTGT+GGG 0.538137 7_3:+32363541 Msa1101580:CDS
AATGAAGGTTTCTTCACCTG+TGG 0.539316 7_3:+32363540 Msa1101580:CDS
ACCTTCCGATCTGAAACTAC+TGG 0.540836 7_3:+32362365 Msa1101580:five_prime_UTR
CCAATCGATGAAGATGAAGC+TGG 0.542345 7_3:+32362190 Msa1101580:five_prime_UTR
AAATTAAAGGAACTCACAGT+GGG 0.551591 7_3:-32363853 None:intergenic
GATTGGGTAAAAGTTGAAGA+AGG 0.561791 7_3:+32364079 Msa1101580:three_prime_UTR
GAATTGCAAACCTTCCATAG+AGG 0.570742 7_3:-32363296 None:intergenic
ACAAGTCACCATCAATATGA+AGG 0.583661 7_3:-32364291 None:intergenic
TATTTGTAGCTATTCAAAGT+GGG 0.583785 7_3:-32363817 None:intergenic
TGAATGAATCAAAACTGGAT+CGG 0.587823 7_3:-32362276 None:intergenic
AGTTGTCCCTTCTGGATTTG+GGG 0.591313 7_3:+32363571 Msa1101580:CDS
GCCAGTAGTTTCAGATCGGA+AGG 0.593019 7_3:-32362366 None:intergenic
CTGAGCCAGTAGTTTCAGAT+CGG 0.609401 7_3:-32362370 None:intergenic
ACACCAACCCCAAATCCAGA+AGG 0.616176 7_3:-32363578 None:intergenic
ATATTTGTAGCTATTCAAAG+TGG 0.616310 7_3:-32363818 None:intergenic
TGGAAGATCTCCTCTATGGA+AGG 0.616327 7_3:+32363286 Msa1101580:CDS
ATTGTGTTAAAAGAACTTTG+AGG 0.627012 7_3:+32363505 Msa1101580:CDS
GGACAACTTCATCTCCCCAC+AGG 0.640268 7_3:-32363556 None:intergenic
ATTGGCAAAAGAGTAAAAGA+CGG 0.645409 7_3:+32364124 Msa1101580:three_prime_UTR
CAAATTAAAGGAACTCACAG+TGG 0.650332 7_3:-32363854 None:intergenic
CACCAACCCCAAATCCAGAA+GGG 0.652893 7_3:-32363577 None:intergenic
TGAAGGTTTCTTCACCTGTG+GGG 0.665032 7_3:+32363542 Msa1101580:CDS
AGTTGAAGAAGGCAACGACA+AGG 0.678134 7_3:+32364090 Msa1101580:three_prime_UTR
TGGTACTAGAGAGTCAGGCA+CGG 0.717302 7_3:-32363906 None:intergenic
TGGCAAAAGAGTAAAAGACG+GGG 0.756073 7_3:+32364126 Msa1101580:three_prime_UTR
AATTAAAGGAACTCACAGTG+GGG 0.782022 7_3:-32363852 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATTATAAAATATAAT+AGG - chr7_3:32364366-32364385 None:intergenic 0.0%
!! AATTTATTATAAAATATAAT+AGG - chr7_3:32364366-32364385 None:intergenic 0.0%
!! TATTTGTTTAATATGAAAAT+TGG + chr7_3:32363196-32363215 Msa1101580:intron 10.0%
!! TATTTGTTTAATATGAAAAT+TGG + chr7_3:32363196-32363215 Msa1101580:intron 10.0%
!!! TTTATCAACAACAAATTTTT+GGG - chr7_3:32362535-32362554 None:intergenic 15.0%
!!! ATTTATCAACAACAAATTTT+TGG - chr7_3:32362536-32362555 None:intergenic 15.0%
!! TTATGAAAATAAATAGAACA+AGG - chr7_3:32363414-32363433 None:intergenic 15.0%
!!! ATTTTCATAATTTCAATTTG+AGG + chr7_3:32363424-32363443 Msa1101580:intron 15.0%
!! ATAAAGTTTATGCAAATTAA+AGG - chr7_3:32363869-32363888 None:intergenic 15.0%
!!! TTTATCAACAACAAATTTTT+GGG - chr7_3:32362535-32362554 None:intergenic 15.0%
!!! ATTTATCAACAACAAATTTT+TGG - chr7_3:32362536-32362555 None:intergenic 15.0%
!! TTATGAAAATAAATAGAACA+AGG - chr7_3:32363414-32363433 None:intergenic 15.0%
!!! ATTTTCATAATTTCAATTTG+AGG + chr7_3:32363424-32363443 Msa1101580:intron 15.0%
!! ATAAAGTTTATGCAAATTAA+AGG - chr7_3:32363869-32363888 None:intergenic 15.0%
!!! TGAAAATTGAATTTTAGAGA+AGG + chr7_3:32362650-32362669 Msa1101580:intron 20.0%
!!! TTTTAGAGAACTTATGAAAA+CGG + chr7_3:32362939-32362958 Msa1101580:intron 20.0%
!! ATAAAGATATGTCTACAATT+TGG + chr7_3:32363338-32363357 Msa1101580:intron 20.0%
!! TAAAAAGAAAAACAGAATTC+TGG - chr7_3:32363929-32363948 None:intergenic 20.0%
!!! GAATTCTGTTTTTCTTTTTA+AGG + chr7_3:32363929-32363948 Msa1101580:three_prime_UTR 20.0%
!! ACAAAACACTAGTTATATTA+AGG - chr7_3:32364227-32364246 None:intergenic 20.0%
!!! ATGTATTTTATGACTAATGT+CGG + chr7_3:32364332-32364351 Msa1101580:three_prime_UTR 20.0%
!!! TGAAAATTGAATTTTAGAGA+AGG + chr7_3:32362650-32362669 Msa1101580:intron 20.0%
!!! TTTTAGAGAACTTATGAAAA+CGG + chr7_3:32362939-32362958 Msa1101580:intron 20.0%
!! ATAAAGATATGTCTACAATT+TGG + chr7_3:32363338-32363357 Msa1101580:intron 20.0%
!! TAAAAAGAAAAACAGAATTC+TGG - chr7_3:32363929-32363948 None:intergenic 20.0%
!!! GAATTCTGTTTTTCTTTTTA+AGG + chr7_3:32363929-32363948 Msa1101580:three_prime_UTR 20.0%
!! ACAAAACACTAGTTATATTA+AGG - chr7_3:32364227-32364246 None:intergenic 20.0%
!!! ATGTATTTTATGACTAATGT+CGG + chr7_3:32364332-32364351 Msa1101580:three_prime_UTR 20.0%
! ATCAATGAATGAATCAAAAC+TGG - chr7_3:32362284-32362303 None:intergenic 25.0%
! AGTGATATGTATATTTGACT+TGG + chr7_3:32362746-32362765 Msa1101580:intron 25.0%
! ATTACAAACTACTATGAGTT+TGG + chr7_3:32362791-32362810 Msa1101580:intron 25.0%
!! ACTTATTTGACTTTATGTAG+TGG + chr7_3:32362856-32362875 Msa1101580:intron 25.0%
!! TTTATGTAGTGGAATAAGTT+TGG + chr7_3:32362867-32362886 Msa1101580:intron 25.0%
!!! TGACTTATTTTACTTATCTC+AGG + chr7_3:32362902-32362921 Msa1101580:intron 25.0%
!!! GACTTATTTTACTTATCTCA+GGG + chr7_3:32362903-32362922 Msa1101580:intron 25.0%
!! TTCTCTAAAAATAGCCAAAT+AGG - chr7_3:32362929-32362948 None:intergenic 25.0%
!!! GGCTTTGAATATAATGATAT+AGG - chr7_3:32363112-32363131 None:intergenic 25.0%
! AACCTAAAAATAGCACATAT+TGG - chr7_3:32363133-32363152 None:intergenic 25.0%
! TCTAATCAATATGTCATTGA+AGG - chr7_3:32363163-32363182 None:intergenic 25.0%
!! GTCTACAATTTGGAAAATTT+TGG + chr7_3:32363348-32363367 Msa1101580:intron 25.0%
! ATTGTGTTAAAAGAACTTTG+AGG + chr7_3:32363505-32363524 Msa1101580:CDS 25.0%
! AGAAACCTTCATTAAATTCA+GGG - chr7_3:32363533-32363552 None:intergenic 25.0%
! AAGAAACCTTCATTAAATTC+AGG - chr7_3:32363534-32363553 None:intergenic 25.0%
!!! CATATGGTTTTTTGATTGTT+GGG - chr7_3:32363605-32363624 None:intergenic 25.0%
!!! TCATATGGTTTTTTGATTGT+TGG - chr7_3:32363606-32363625 None:intergenic 25.0%
! GTAGTTGTATGTATATCATA+TGG - chr7_3:32363621-32363640 None:intergenic 25.0%
! TATTTGTAGCTATTCAAAGT+GGG - chr7_3:32363820-32363839 None:intergenic 25.0%
! ATATTTGTAGCTATTCAAAG+TGG - chr7_3:32363821-32363840 None:intergenic 25.0%
! GAGTAAAAGAAAAACTCTAA+AGG - chr7_3:32364008-32364027 None:intergenic 25.0%
! TTTGTGTTTGATCAATCATA+TGG + chr7_3:32364049-32364068 Msa1101580:three_prime_UTR 25.0%
!!! AATCATATGGTTTTGTAGAT+TGG + chr7_3:32364062-32364081 Msa1101580:three_prime_UTR 25.0%
!!! ATCATATGGTTTTGTAGATT+GGG + chr7_3:32364063-32364082 Msa1101580:three_prime_UTR 25.0%
!! ATTTTACACCTTCATATTGA+TGG + chr7_3:32364283-32364302 Msa1101580:three_prime_UTR 25.0%
!! TGTATTTTATGACTAATGTC+GGG + chr7_3:32364333-32364352 Msa1101580:three_prime_UTR 25.0%
! ATCAATGAATGAATCAAAAC+TGG - chr7_3:32362284-32362303 None:intergenic 25.0%
! AGTGATATGTATATTTGACT+TGG + chr7_3:32362746-32362765 Msa1101580:intron 25.0%
! ATTACAAACTACTATGAGTT+TGG + chr7_3:32362791-32362810 Msa1101580:intron 25.0%
!! ACTTATTTGACTTTATGTAG+TGG + chr7_3:32362856-32362875 Msa1101580:intron 25.0%
!! TTTATGTAGTGGAATAAGTT+TGG + chr7_3:32362867-32362886 Msa1101580:intron 25.0%
!!! TGACTTATTTTACTTATCTC+AGG + chr7_3:32362902-32362921 Msa1101580:intron 25.0%
!!! GACTTATTTTACTTATCTCA+GGG + chr7_3:32362903-32362922 Msa1101580:intron 25.0%
!! TTCTCTAAAAATAGCCAAAT+AGG - chr7_3:32362929-32362948 None:intergenic 25.0%
!!! GGCTTTGAATATAATGATAT+AGG - chr7_3:32363112-32363131 None:intergenic 25.0%
! AACCTAAAAATAGCACATAT+TGG - chr7_3:32363133-32363152 None:intergenic 25.0%
! TCTAATCAATATGTCATTGA+AGG - chr7_3:32363163-32363182 None:intergenic 25.0%
!! GTCTACAATTTGGAAAATTT+TGG + chr7_3:32363348-32363367 Msa1101580:intron 25.0%
! ATTGTGTTAAAAGAACTTTG+AGG + chr7_3:32363505-32363524 Msa1101580:CDS 25.0%
! AGAAACCTTCATTAAATTCA+GGG - chr7_3:32363533-32363552 None:intergenic 25.0%
! AAGAAACCTTCATTAAATTC+AGG - chr7_3:32363534-32363553 None:intergenic 25.0%
!!! CATATGGTTTTTTGATTGTT+GGG - chr7_3:32363605-32363624 None:intergenic 25.0%
!!! TCATATGGTTTTTTGATTGT+TGG - chr7_3:32363606-32363625 None:intergenic 25.0%
! GTAGTTGTATGTATATCATA+TGG - chr7_3:32363621-32363640 None:intergenic 25.0%
! TATTTGTAGCTATTCAAAGT+GGG - chr7_3:32363820-32363839 None:intergenic 25.0%
! ATATTTGTAGCTATTCAAAG+TGG - chr7_3:32363821-32363840 None:intergenic 25.0%
! GAGTAAAAGAAAAACTCTAA+AGG - chr7_3:32364008-32364027 None:intergenic 25.0%
! TTTGTGTTTGATCAATCATA+TGG + chr7_3:32364049-32364068 Msa1101580:three_prime_UTR 25.0%
!!! AATCATATGGTTTTGTAGAT+TGG + chr7_3:32364062-32364081 Msa1101580:three_prime_UTR 25.0%
!!! ATCATATGGTTTTGTAGATT+GGG + chr7_3:32364063-32364082 Msa1101580:three_prime_UTR 25.0%
!! ATTTTACACCTTCATATTGA+TGG + chr7_3:32364283-32364302 Msa1101580:three_prime_UTR 25.0%
!! TGTATTTTATGACTAATGTC+GGG + chr7_3:32364333-32364352 Msa1101580:three_prime_UTR 25.0%
GAGAAAGGAATTTGGAAAAT+TGG - chr7_3:32362134-32362153 None:intergenic 30.0%
! ATCTTCATCGATTGGAAATT+TGG - chr7_3:32362185-32362204 None:intergenic 30.0%
TGAATGAATCAAAACTGGAT+CGG - chr7_3:32362279-32362298 None:intergenic 30.0%
ATCAACAAGTAGTTTCAGAT+CGG - chr7_3:32362402-32362421 None:intergenic 30.0%
TATCTGTGAACGAAAATTTC+AGG + chr7_3:32362463-32362482 Msa1101580:intron 30.0%
!! CATTGATTTGAAGCTGTATA+AGG + chr7_3:32362493-32362512 Msa1101580:CDS 30.0%
TGTATAAGGAAACAAATCTC+AGG + chr7_3:32362507-32362526 Msa1101580:CDS 30.0%
GCTGAAACAAATTCGAAATT+AGG - chr7_3:32362611-32362630 None:intergenic 30.0%
TTTACTTTGTCTGCTATGAT+TGG + chr7_3:32362711-32362730 Msa1101580:intron 30.0%
TTACTTTGTCTGCTATGATT+GGG + chr7_3:32362712-32362731 Msa1101580:intron 30.0%
!! GATATGTATATTTGACTTGG+TGG + chr7_3:32362749-32362768 Msa1101580:intron 30.0%
!!! ATTGCACTATTTTTAGCTGA+TGG + chr7_3:32363004-32363023 Msa1101580:intron 30.0%
!!! TTGCACTATTTTTAGCTGAT+GGG + chr7_3:32363005-32363024 Msa1101580:intron 30.0%
!!! AGCCAATATGTGCTATTTTT+AGG + chr7_3:32363128-32363147 Msa1101580:intron 30.0%
AATTTGATATCCAGACAACT+TGG + chr7_3:32363255-32363274 Msa1101580:intron 30.0%
CTGCTATTAGCATCAAATAT+AGG - chr7_3:32363483-32363502 None:intergenic 30.0%
AAATTAAAGGAACTCACAGT+GGG - chr7_3:32363856-32363875 None:intergenic 30.0%
! TTTTCTTTTACTCTTCTCAG+AGG + chr7_3:32364015-32364034 Msa1101580:three_prime_UTR 30.0%
ATTGGCAAAAGAGTAAAAGA+CGG + chr7_3:32364124-32364143 Msa1101580:three_prime_UTR 30.0%
AAGACTAAGAGTAGAAGATA+GGG + chr7_3:32364164-32364183 Msa1101580:three_prime_UTR 30.0%
GAGAAAGGAATTTGGAAAAT+TGG - chr7_3:32362134-32362153 None:intergenic 30.0%
! ATCTTCATCGATTGGAAATT+TGG - chr7_3:32362185-32362204 None:intergenic 30.0%
TGAATGAATCAAAACTGGAT+CGG - chr7_3:32362279-32362298 None:intergenic 30.0%
ATCAACAAGTAGTTTCAGAT+CGG - chr7_3:32362402-32362421 None:intergenic 30.0%
TATCTGTGAACGAAAATTTC+AGG + chr7_3:32362463-32362482 Msa1101580:intron 30.0%
!! CATTGATTTGAAGCTGTATA+AGG + chr7_3:32362493-32362512 Msa1101580:CDS 30.0%
TGTATAAGGAAACAAATCTC+AGG + chr7_3:32362507-32362526 Msa1101580:CDS 30.0%
GCTGAAACAAATTCGAAATT+AGG - chr7_3:32362611-32362630 None:intergenic 30.0%
TTTACTTTGTCTGCTATGAT+TGG + chr7_3:32362711-32362730 Msa1101580:intron 30.0%
TTACTTTGTCTGCTATGATT+GGG + chr7_3:32362712-32362731 Msa1101580:intron 30.0%
!! GATATGTATATTTGACTTGG+TGG + chr7_3:32362749-32362768 Msa1101580:intron 30.0%
!!! ATTGCACTATTTTTAGCTGA+TGG + chr7_3:32363004-32363023 Msa1101580:intron 30.0%
!!! TTGCACTATTTTTAGCTGAT+GGG + chr7_3:32363005-32363024 Msa1101580:intron 30.0%
!!! AGCCAATATGTGCTATTTTT+AGG + chr7_3:32363128-32363147 Msa1101580:intron 30.0%
AATTTGATATCCAGACAACT+TGG + chr7_3:32363255-32363274 Msa1101580:intron 30.0%
CTGCTATTAGCATCAAATAT+AGG - chr7_3:32363483-32363502 None:intergenic 30.0%
AAATTAAAGGAACTCACAGT+GGG - chr7_3:32363856-32363875 None:intergenic 30.0%
! TTTTCTTTTACTCTTCTCAG+AGG + chr7_3:32364015-32364034 Msa1101580:three_prime_UTR 30.0%
ATTGGCAAAAGAGTAAAAGA+CGG + chr7_3:32364124-32364143 Msa1101580:three_prime_UTR 30.0%
AAGACTAAGAGTAGAAGATA+GGG + chr7_3:32364164-32364183 Msa1101580:three_prime_UTR 30.0%
GATTGGATCTTCAAAACGAT+TGG - chr7_3:32362221-32362240 None:intergenic 35.0%
AAACGAGATTGGAAAACGAT+TGG - chr7_3:32362255-32362274 None:intergenic 35.0%
! CAGTACAAGTTGAAGTTGAT+TGG + chr7_3:32362561-32362580 Msa1101580:intron 35.0%
TGTTCGATGAAATGATTGTG+AGG + chr7_3:32362682-32362701 Msa1101580:intron 35.0%
TACTTTGTCTGCTATGATTG+GGG + chr7_3:32362713-32362732 Msa1101580:intron 35.0%
TACTATGAGTTTGGTATCTC+AGG + chr7_3:32362800-32362819 Msa1101580:intron 35.0%
ACTATGAGTTTGGTATCTCA+GGG + chr7_3:32362801-32362820 Msa1101580:intron 35.0%
TAAACTGAAACAGTCCAAAC+AGG - chr7_3:32362827-32362846 None:intergenic 35.0%
!!! TGCACTATTTTTAGCTGATG+GGG + chr7_3:32363006-32363025 Msa1101580:intron 35.0%
AGTGGCAAGATATACTATCA+AGG + chr7_3:32363037-32363056 Msa1101580:intron 35.0%
! CTGCAATGCTATATGTTTTC+AGG + chr7_3:32363072-32363091 Msa1101580:intron 35.0%
ACAACTACAGCTACAATTAC+AGG + chr7_3:32363633-32363652 Msa1101580:CDS 35.0%
!!! AATGAAATGCTGCTTTTTCC+GGG - chr7_3:32363711-32363730 None:intergenic 35.0%
!!! CAATGAAATGCTGCTTTTTC+CGG - chr7_3:32363712-32363731 None:intergenic 35.0%
AAGCAGCATTTCATTGAGTT+AGG + chr7_3:32363716-32363735 Msa1101580:CDS 35.0%
AATTAAAGGAACTCACAGTG+GGG - chr7_3:32363855-32363874 None:intergenic 35.0%
CAAATTAAAGGAACTCACAG+TGG - chr7_3:32363857-32363876 None:intergenic 35.0%
!! CTAAAGGCACTTCATTGTTT+AGG - chr7_3:32363992-32364011 None:intergenic 35.0%
GATTGGGTAAAAGTTGAAGA+AGG + chr7_3:32364079-32364098 Msa1101580:three_prime_UTR 35.0%
TTGGCAAAAGAGTAAAAGAC+GGG + chr7_3:32364125-32364144 Msa1101580:three_prime_UTR 35.0%
CAAGACTAAGAGTAGAAGAT+AGG + chr7_3:32364163-32364182 Msa1101580:three_prime_UTR 35.0%
ACAAGTCACCATCAATATGA+AGG - chr7_3:32364294-32364313 None:intergenic 35.0%
GATTGGATCTTCAAAACGAT+TGG - chr7_3:32362221-32362240 None:intergenic 35.0%
AAACGAGATTGGAAAACGAT+TGG - chr7_3:32362255-32362274 None:intergenic 35.0%
! CAGTACAAGTTGAAGTTGAT+TGG + chr7_3:32362561-32362580 Msa1101580:intron 35.0%
TGTTCGATGAAATGATTGTG+AGG + chr7_3:32362682-32362701 Msa1101580:intron 35.0%
TACTTTGTCTGCTATGATTG+GGG + chr7_3:32362713-32362732 Msa1101580:intron 35.0%
TACTATGAGTTTGGTATCTC+AGG + chr7_3:32362800-32362819 Msa1101580:intron 35.0%
ACTATGAGTTTGGTATCTCA+GGG + chr7_3:32362801-32362820 Msa1101580:intron 35.0%
TAAACTGAAACAGTCCAAAC+AGG - chr7_3:32362827-32362846 None:intergenic 35.0%
!!! TGCACTATTTTTAGCTGATG+GGG + chr7_3:32363006-32363025 Msa1101580:intron 35.0%
AGTGGCAAGATATACTATCA+AGG + chr7_3:32363037-32363056 Msa1101580:intron 35.0%
! CTGCAATGCTATATGTTTTC+AGG + chr7_3:32363072-32363091 Msa1101580:intron 35.0%
ACAACTACAGCTACAATTAC+AGG + chr7_3:32363633-32363652 Msa1101580:CDS 35.0%
!!! AATGAAATGCTGCTTTTTCC+GGG - chr7_3:32363711-32363730 None:intergenic 35.0%
!!! CAATGAAATGCTGCTTTTTC+CGG - chr7_3:32363712-32363731 None:intergenic 35.0%
AAGCAGCATTTCATTGAGTT+AGG + chr7_3:32363716-32363735 Msa1101580:CDS 35.0%
AATTAAAGGAACTCACAGTG+GGG - chr7_3:32363855-32363874 None:intergenic 35.0%
CAAATTAAAGGAACTCACAG+TGG - chr7_3:32363857-32363876 None:intergenic 35.0%
!! CTAAAGGCACTTCATTGTTT+AGG - chr7_3:32363992-32364011 None:intergenic 35.0%
GATTGGGTAAAAGTTGAAGA+AGG + chr7_3:32364079-32364098 Msa1101580:three_prime_UTR 35.0%
TTGGCAAAAGAGTAAAAGAC+GGG + chr7_3:32364125-32364144 Msa1101580:three_prime_UTR 35.0%
CAAGACTAAGAGTAGAAGAT+AGG + chr7_3:32364163-32364182 Msa1101580:three_prime_UTR 35.0%
ACAAGTCACCATCAATATGA+AGG - chr7_3:32364294-32364313 None:intergenic 35.0%
! GTCTGAAACGAGAAAGAGAA+AGG - chr7_3:32362085-32362104 None:intergenic 40.0%
TGGAGAAAGAGAGAGAAAGA+TGG - chr7_3:32362114-32362133 None:intergenic 40.0%
ACGAGAGAGAGAAAGGAATT+TGG - chr7_3:32362142-32362161 None:intergenic 40.0%
! GTTTGAAACGAGAGAGAGAA+AGG - chr7_3:32362149-32362168 None:intergenic 40.0%
ATCGATTGACGATGAAGGAT+TGG - chr7_3:32362324-32362343 None:intergenic 40.0%
TACTTATCTCAGGGCCTATT+TGG + chr7_3:32362912-32362931 Msa1101580:intron 40.0%
GAATTGCAAACCTTCCATAG+AGG - chr7_3:32363299-32363318 None:intergenic 40.0%
AGGAGCCCTGAATTTAATGA+AGG + chr7_3:32363525-32363544 Msa1101580:CDS 40.0%
AATGAAGGTTTCTTCACCTG+TGG + chr7_3:32363540-32363559 Msa1101580:CDS 40.0%
ATGAAGGTTTCTTCACCTGT+GGG + chr7_3:32363541-32363560 Msa1101580:CDS 40.0%
AAGTTGTCCCTTCTGGATTT+GGG + chr7_3:32363570-32363589 Msa1101580:CDS 40.0%
AATTCTGGTACTAGAGAGTC+AGG - chr7_3:32363914-32363933 None:intergenic 40.0%
GACAAGGCTTGAGAAACAAT+TGG + chr7_3:32364106-32364125 Msa1101580:three_prime_UTR 40.0%
TGGCAAAAGAGTAAAAGACG+GGG + chr7_3:32364126-32364145 Msa1101580:three_prime_UTR 40.0%
TAAAAGACGGGGCAATTACA+AGG + chr7_3:32364137-32364156 Msa1101580:three_prime_UTR 40.0%
! GTCTGAAACGAGAAAGAGAA+AGG - chr7_3:32362085-32362104 None:intergenic 40.0%
TGGAGAAAGAGAGAGAAAGA+TGG - chr7_3:32362114-32362133 None:intergenic 40.0%
ACGAGAGAGAGAAAGGAATT+TGG - chr7_3:32362142-32362161 None:intergenic 40.0%
! GTTTGAAACGAGAGAGAGAA+AGG - chr7_3:32362149-32362168 None:intergenic 40.0%
ATCGATTGACGATGAAGGAT+TGG - chr7_3:32362324-32362343 None:intergenic 40.0%
TACTTATCTCAGGGCCTATT+TGG + chr7_3:32362912-32362931 Msa1101580:intron 40.0%
GAATTGCAAACCTTCCATAG+AGG - chr7_3:32363299-32363318 None:intergenic 40.0%
AGGAGCCCTGAATTTAATGA+AGG + chr7_3:32363525-32363544 Msa1101580:CDS 40.0%
AATGAAGGTTTCTTCACCTG+TGG + chr7_3:32363540-32363559 Msa1101580:CDS 40.0%
ATGAAGGTTTCTTCACCTGT+GGG + chr7_3:32363541-32363560 Msa1101580:CDS 40.0%
AAGTTGTCCCTTCTGGATTT+GGG + chr7_3:32363570-32363589 Msa1101580:CDS 40.0%
AATTCTGGTACTAGAGAGTC+AGG - chr7_3:32363914-32363933 None:intergenic 40.0%
GACAAGGCTTGAGAAACAAT+TGG + chr7_3:32364106-32364125 Msa1101580:three_prime_UTR 40.0%
TGGCAAAAGAGTAAAAGACG+GGG + chr7_3:32364126-32364145 Msa1101580:three_prime_UTR 40.0%
TAAAAGACGGGGCAATTACA+AGG + chr7_3:32364137-32364156 Msa1101580:three_prime_UTR 40.0%
CCAGCTTCATCTTCATCGAT+TGG - chr7_3:32362193-32362212 None:intergenic 45.0%
CCAATCGATGAAGATGAAGC+TGG + chr7_3:32362190-32362209 Msa1101580:five_prime_UTR 45.0%
GATTGGAGCTTCATGCTGAT+TGG - chr7_3:32362238-32362257 None:intergenic 45.0%
ACTGGATCGGAAAACGAGAT+TGG - chr7_3:32362266-32362285 None:intergenic 45.0%
GACGAATCGATTGACGATGA+AGG - chr7_3:32362329-32362348 None:intergenic 45.0%
! ACCTTCCGATCTGAAACTAC+TGG + chr7_3:32362365-32362384 Msa1101580:five_prime_UTR 45.0%
CTGAGCCAGTAGTTTCAGAT+CGG - chr7_3:32362373-32362392 None:intergenic 45.0%
CTGCTATGATTGGGGCTTAT+AGG + chr7_3:32362721-32362740 Msa1101580:intron 45.0%
TGGAAGATCTCCTCTATGGA+AGG + chr7_3:32363286-32363305 Msa1101580:CDS 45.0%
TGAAGGTTTCTTCACCTGTG+GGG + chr7_3:32363542-32363561 Msa1101580:CDS 45.0%
GAAGTTGTCCCTTCTGGATT+TGG + chr7_3:32363569-32363588 Msa1101580:CDS 45.0%
AGTTGTCCCTTCTGGATTTG+GGG + chr7_3:32363571-32363590 Msa1101580:CDS 45.0%
AATGTGTGATGCTGCTGTCT+TGG + chr7_3:32363788-32363807 Msa1101580:three_prime_UTR 45.0%
AGTTGAAGAAGGCAACGACA+AGG + chr7_3:32364090-32364109 Msa1101580:three_prime_UTR 45.0%
CCAGCTTCATCTTCATCGAT+TGG - chr7_3:32362193-32362212 None:intergenic 45.0%
CCAATCGATGAAGATGAAGC+TGG + chr7_3:32362190-32362209 Msa1101580:five_prime_UTR 45.0%
GATTGGAGCTTCATGCTGAT+TGG - chr7_3:32362238-32362257 None:intergenic 45.0%
ACTGGATCGGAAAACGAGAT+TGG - chr7_3:32362266-32362285 None:intergenic 45.0%
GACGAATCGATTGACGATGA+AGG - chr7_3:32362329-32362348 None:intergenic 45.0%
! ACCTTCCGATCTGAAACTAC+TGG + chr7_3:32362365-32362384 Msa1101580:five_prime_UTR 45.0%
CTGAGCCAGTAGTTTCAGAT+CGG - chr7_3:32362373-32362392 None:intergenic 45.0%
CTGCTATGATTGGGGCTTAT+AGG + chr7_3:32362721-32362740 Msa1101580:intron 45.0%
TGGAAGATCTCCTCTATGGA+AGG + chr7_3:32363286-32363305 Msa1101580:CDS 45.0%
TGAAGGTTTCTTCACCTGTG+GGG + chr7_3:32363542-32363561 Msa1101580:CDS 45.0%
GAAGTTGTCCCTTCTGGATT+TGG + chr7_3:32363569-32363588 Msa1101580:CDS 45.0%
AGTTGTCCCTTCTGGATTTG+GGG + chr7_3:32363571-32363590 Msa1101580:CDS 45.0%
AATGTGTGATGCTGCTGTCT+TGG + chr7_3:32363788-32363807 Msa1101580:three_prime_UTR 45.0%
AGTTGAAGAAGGCAACGACA+AGG + chr7_3:32364090-32364109 Msa1101580:three_prime_UTR 45.0%
!! CAGATCGGAAGGTGTTCGAT+TGG - chr7_3:32362358-32362377 None:intergenic 50.0%
GCCAGTAGTTTCAGATCGGA+AGG - chr7_3:32362369-32362388 None:intergenic 50.0%
!! CGATCTGAAACTACTGGCTC+AGG + chr7_3:32362371-32362390 Msa1101580:five_prime_UTR 50.0%
TTGGTATCTCAGGGCCTGTT+TGG + chr7_3:32362810-32362829 Msa1101580:intron 50.0%
!!! TTTTTAGCTGATGGGGCTGC+TGG + chr7_3:32363013-32363032 Msa1101580:intron 50.0%
CAGCTGGAAGATCTCCTCTA+TGG + chr7_3:32363282-32363301 Msa1101580:CDS 50.0%
GGAGATGAAGTTGTCCCTTC+TGG + chr7_3:32363563-32363582 Msa1101580:CDS 50.0%
CACCAACCCCAAATCCAGAA+GGG - chr7_3:32363580-32363599 None:intergenic 50.0%
ACACCAACCCCAAATCCAGA+AGG - chr7_3:32363581-32363600 None:intergenic 50.0%
CTCTATCCTGTCATTGCACC+CGG + chr7_3:32363690-32363709 Msa1101580:CDS 50.0%
! CTTTTTCCGGGTGCAATGAC+AGG - chr7_3:32363699-32363718 None:intergenic 50.0%
TGGTACTAGAGAGTCAGGCA+CGG - chr7_3:32363909-32363928 None:intergenic 50.0%
!! CAGATCGGAAGGTGTTCGAT+TGG - chr7_3:32362358-32362377 None:intergenic 50.0%
GCCAGTAGTTTCAGATCGGA+AGG - chr7_3:32362369-32362388 None:intergenic 50.0%
!! CGATCTGAAACTACTGGCTC+AGG + chr7_3:32362371-32362390 Msa1101580:five_prime_UTR 50.0%
TTGGTATCTCAGGGCCTGTT+TGG + chr7_3:32362810-32362829 Msa1101580:intron 50.0%
!!! TTTTTAGCTGATGGGGCTGC+TGG + chr7_3:32363013-32363032 Msa1101580:intron 50.0%
CAGCTGGAAGATCTCCTCTA+TGG + chr7_3:32363282-32363301 Msa1101580:CDS 50.0%
GGAGATGAAGTTGTCCCTTC+TGG + chr7_3:32363563-32363582 Msa1101580:CDS 50.0%
CACCAACCCCAAATCCAGAA+GGG - chr7_3:32363580-32363599 None:intergenic 50.0%
ACACCAACCCCAAATCCAGA+AGG - chr7_3:32363581-32363600 None:intergenic 50.0%
CTCTATCCTGTCATTGCACC+CGG + chr7_3:32363690-32363709 Msa1101580:CDS 50.0%
! CTTTTTCCGGGTGCAATGAC+AGG - chr7_3:32363699-32363718 None:intergenic 50.0%
TGGTACTAGAGAGTCAGGCA+CGG - chr7_3:32363909-32363928 None:intergenic 50.0%
CAGCTGCAGTCCAAGTTGTC+TGG - chr7_3:32363268-32363287 None:intergenic 55.0%
CAGACAACTTGGACTGCAGC+TGG + chr7_3:32363266-32363285 Msa1101580:intron 55.0%
GGACAACTTCATCTCCCCAC+AGG - chr7_3:32363559-32363578 None:intergenic 55.0%
! GTCCCTTCTGGATTTGGGGT+TGG + chr7_3:32363575-32363594 Msa1101580:CDS 55.0%
CAGCTGCAGTCCAAGTTGTC+TGG - chr7_3:32363268-32363287 None:intergenic 55.0%
CAGACAACTTGGACTGCAGC+TGG + chr7_3:32363266-32363285 Msa1101580:intron 55.0%
GGACAACTTCATCTCCCCAC+AGG - chr7_3:32363559-32363578 None:intergenic 55.0%
! GTCCCTTCTGGATTTGGGGT+TGG + chr7_3:32363575-32363594 Msa1101580:CDS 55.0%
GCTGATGGGGCTGCTGGAAG+TGG + chr7_3:32363019-32363038 Msa1101580:intron 65.0%
GCTGATGGGGCTGCTGGAAG+TGG + chr7_3:32363019-32363038 Msa1101580:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 32362031 32364422 32362031 ID=Msa1101580;Name=Msa1101580
chr7_3 mRNA 32362031 32364422 32362031 ID=Msa1101580-mRNA-1;Parent=Msa1101580;Name=Msa1101580-mRNA-1;_AED=0.10;_eAED=0.10;_QI=365|0.66|1|1|1|1|4|685|104
chr7_3 exon 32362031 32362392 32362031 ID=Msa1101580-mRNA-1:exon:6968;Parent=Msa1101580-mRNA-1
chr7_3 exon 32362485 32362528 32362485 ID=Msa1101580-mRNA-1:exon:6969;Parent=Msa1101580-mRNA-1
chr7_3 exon 32363269 32363307 32363269 ID=Msa1101580-mRNA-1:exon:6970;Parent=Msa1101580-mRNA-1
chr7_3 exon 32363503 32364422 32363503 ID=Msa1101580-mRNA-1:exon:6971;Parent=Msa1101580-mRNA-1
chr7_3 five_prime_UTR 32362031 32362392 32362031 ID=Msa1101580-mRNA-1:five_prime_utr;Parent=Msa1101580-mRNA-1
chr7_3 five_prime_UTR 32362485 32362487 32362485 ID=Msa1101580-mRNA-1:five_prime_utr;Parent=Msa1101580-mRNA-1
chr7_3 CDS 32362488 32362528 32362488 ID=Msa1101580-mRNA-1:cds;Parent=Msa1101580-mRNA-1
chr7_3 CDS 32363269 32363307 32363269 ID=Msa1101580-mRNA-1:cds;Parent=Msa1101580-mRNA-1
chr7_3 CDS 32363503 32363737 32363503 ID=Msa1101580-mRNA-1:cds;Parent=Msa1101580-mRNA-1
chr7_3 three_prime_UTR 32363738 32364422 32363738 ID=Msa1101580-mRNA-1:three_prime_utr;Parent=Msa1101580-mRNA-1
Gene Sequence

>Msa1101580

ATGTGCATTGATTTGAAGCTGTATAAGGAAACAAATCTCAGACAACTTGGACTGCAGCTGGAAGATCTCCTCTATGGAAGAAATTGTGTTAAAAGAACTTTGAGGAGCCCTGAATTTAATGAAGGTTTCTTCACCTGTGGGGAGATGAAGTTGTCCCTTCTGGATTTGGGGTTGGTGTTCCCAACAATCAAAAAACCATATGATATACATACAACTACAGCTACAATTACAGGCAAGTCTATCTCTTTTTATCTAAATTCTCTACATCTCTATCCTGTCATTGCACCCGGAAAAAGCAGCATTTCATTGAGTTAG
Protein sequence

>Msa1101580

MCIDLKLYKETNLRQLGLQLEDLLYGRNCVKRTLRSPEFNEGFFTCGEMKLSLLDLGLVFPTIKKPYDIHTTTATITGKSISFYLNSLHLYPVIAPGKSSISLS*