AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1197600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1197600 MtrunA17_Chr8g0366501 98.670 376 3 2 1 374 1 376 0.0 750
Msa1197600 MtrunA17_Chr4g0063401 37.624 202 89 6 89 283 77 248 1.79e-34 127
Msa1197600 MtrunA17_Chr4g0063361 33.333 201 97 5 80 280 76 239 1.92e-27 108
Msa1197600 MtrunA17_Chr4g0063391 28.405 257 115 5 35 284 16 210 1.11e-25 103
Msa1197600 MtrunA17_Chr3g0098251 28.571 224 105 5 60 280 4 175 1.47e-21 91.7
Msa1197600 MtrunA17_Chr4g0063371 36.975 119 46 2 80 198 56 145 9.16e-17 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1197600 AT1G32360.1 60.560 393 122 12 3 374 4 384 4.12e-132 383
Msa1197600 AT3G19360.1 36.755 302 154 10 84 373 98 374 7.08e-46 162
Msa1197600 AT3G19360.2 36.755 302 154 10 84 373 98 374 7.08e-46 162
Msa1197600 AT2G35430.1 41.739 230 63 9 167 374 72 252 1.83e-41 146
Msa1197600 AT2G35430.1 37.427 171 69 7 26 191 31 168 4.13e-20 89.0

Find 143 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGGAGGAGGAGGAGG+TGG 0.204425 8_1:+48259101 Msa1197600:CDS
GGTGGAGGTGGTGGAGGAGG+AGG 0.223597 8_1:+48259095 Msa1197600:CDS
GGAGGTGGTGGAGGAGGAGG+AGG 0.235103 8_1:+48259098 Msa1197600:CDS
TATGAATAATAGGTCTTATA+AGG 0.235365 8_1:+48258941 Msa1197600:CDS
ACTGAAGAAGGTTGTCCTTA+TGG 0.260800 8_1:+48258987 Msa1197600:CDS
GGTGGTGGAGGTGGTGGAGG+AGG 0.265178 8_1:+48259092 Msa1197600:CDS
TATGGTGGTGGTGGAGGTGG+TGG 0.279719 8_1:+48259086 Msa1197600:CDS
CACATTATCAGCATGAGTTT+TGG 0.299688 8_1:-48260645 None:intergenic
GGTGGTGGTGGAGGTGGTGG+AGG 0.301864 8_1:+48259089 Msa1197600:CDS
CAATATTATATTCCCAATCT+TGG 0.304856 8_1:+48260838 Msa1197600:three_prime_UTR
CAATTCGAAGGCTTCAAATT+AGG 0.308899 8_1:-48259200 None:intergenic
AATCTTGGAAACATGGAAAA+TGG 0.310585 8_1:+48260853 Msa1197600:three_prime_UTR
AAATTACAGTTCGTTATATA+AGG 0.316371 8_1:-48258771 None:intergenic
CAGATATGGAGGTGGGCTTA+TGG 0.316746 8_1:+48260562 Msa1197600:CDS
GGTTCAGATGTTTATCATCT+TGG 0.329111 8_1:+48260689 Msa1197600:CDS
AGTTTAGGTCCCGGTGGTTA+TGG 0.330311 8_1:+48259068 Msa1197600:CDS
TTGGATCTAATTTAAGATTC+CGG 0.332594 8_1:+48261150 Msa1197600:three_prime_UTR
GATAAAATCAGTAGGATATA+TGG 0.337131 8_1:+48260785 Msa1197600:CDS
GAAAGTGTGGCGATTAGTTT+AGG 0.346387 8_1:+48259053 Msa1197600:CDS
TTGGGGAGAGTATGAATAAT+AGG 0.346414 8_1:+48258931 Msa1197600:CDS
GGTTATGGTGGTGGTGGAGG+TGG 0.349998 8_1:+48259083 Msa1197600:CDS
CTAATTTGAAGCCTTCGAAT+TGG 0.351114 8_1:+48259201 Msa1197600:CDS
TGCGTCGGCCGCCGGAGAAT+TGG 0.373994 8_1:+48258814 Msa1197600:CDS
TTCTGATGAGCTCTCTGTCC+TGG 0.376334 8_1:-48260749 None:intergenic
CAGCTGGTGCGGCCGCGGTT+GGG 0.377275 8_1:+48259171 Msa1197600:CDS
GCAGCTGGTGCGGCCGCGGT+TGG 0.388240 8_1:+48259170 Msa1197600:CDS
CTGTAGCACCATGAGCAAAA+TGG 0.399102 8_1:-48259283 None:intergenic
TGTAATAAGTGGGAGATGAC+TGG 0.409320 8_1:+48259236 Msa1197600:CDS
GGTGCGGCCGCGGTTGGGAA+TGG 0.416021 8_1:+48259176 Msa1197600:CDS
GGTGGTGGTGGCAGTGGAGG+AGG 0.418521 8_1:+48258672 Msa1197600:CDS
ATGACTGGGTATTGTCCCTT+TGG 0.419828 8_1:+48259251 Msa1197600:CDS
ATGAATAATAGGTCTTATAA+GGG 0.419939 8_1:+48258942 Msa1197600:CDS
GGAGGAGGAGGTGGGAGTGT+TGG 0.423488 8_1:+48259110 Msa1197600:CDS
CGGAAGATGAGTATCGTTAC+TGG 0.423843 8_1:+48258553 Msa1197600:CDS
GGTGTTGTTGTTGTTGCAGC+TGG 0.426984 8_1:+48259155 Msa1197600:CDS
TAATATAACAAGATGGGAAC+TGG 0.428850 8_1:-48260926 None:intergenic
ACTGCAGAATTACACAGATA+TGG 0.430785 8_1:+48260548 Msa1197600:intron
GTTGGTAGTGTTGGTGGTAG+TGG 0.432301 8_1:+48259128 Msa1197600:CDS
CAAGATGGTGGTGGTGGCAG+TGG 0.435000 8_1:+48258666 Msa1197600:CDS
GCGGCGGAGCCGCCGATAGC+TGG 0.440541 8_1:+48258505 Msa1197600:CDS
AAACTAGGATTTGTAATAAG+TGG 0.442008 8_1:+48259225 Msa1197600:CDS
GGTGGGAGTGTTGGTAGTGT+TGG 0.443949 8_1:+48259119 Msa1197600:CDS
GGTCAACCGACCAAGCTGAC+TGG 0.444227 8_1:+48258526 Msa1197600:CDS
TTGCTTCTCGATTTCTTACT+CGG 0.445185 8_1:-48258639 None:intergenic
AGCCTTCGAATTGGAAAACT+AGG 0.448493 8_1:+48259210 Msa1197600:CDS
GCTATCGGCGGCTCCGCCGC+CGG 0.448730 8_1:-48258502 None:intergenic
GGAGTGTGATTGAGCAGCCT+AGG 0.450759 8_1:+48258868 Msa1197600:CDS
GATGGTGGTGGTGGCAGTGG+AGG 0.455113 8_1:+48258669 Msa1197600:CDS
TACTGGAACGGAAACGATGC+TGG 0.458682 8_1:+48258570 Msa1197600:CDS
GGGAGTGTTGGTAGTGTTGG+TGG 0.465014 8_1:+48259122 Msa1197600:CDS
GTGGTGGAGGAGGAGGAGGT+GGG 0.465072 8_1:+48259102 Msa1197600:CDS
GGTGGTTATGGTGGTGGTGG+AGG 0.469378 8_1:+48259080 Msa1197600:CDS
CCGTCTTCAAATTCGCGTGC+AGG 0.475752 8_1:+48258606 Msa1197600:CDS
CCCGGTGGTTATGGTGGTGG+TGG 0.477029 8_1:+48259077 Msa1197600:CDS
GGAGGAGCTGCGTCGGCCGC+CGG 0.477423 8_1:+48258806 Msa1197600:CDS
TGTTGCGTCTGACACAAAAC+AGG 0.484755 8_1:+48260613 Msa1197600:CDS
AGTTTCAGATACCGACGGTT+GGG 0.486443 8_1:+48258895 Msa1197600:CDS
TTGTTCTGTGGATTTATGTT+CGG 0.489657 8_1:+48261012 Msa1197600:three_prime_UTR
CTTCCGTCGCCCAGTCAGCT+TGG 0.490823 8_1:-48258536 None:intergenic
TGTTGCAGCTGGTGCGGCCG+CGG 0.493113 8_1:+48259166 Msa1197600:CDS
TGAACAAAGTGCTACAATGC+TGG 0.495709 8_1:-48260977 None:intergenic
GTCAACCGACCAAGCTGACT+GGG 0.495786 8_1:+48258527 Msa1197600:CDS
AACTAGGATTTGTAATAAGT+GGG 0.496877 8_1:+48259226 Msa1197600:CDS
GTTGCATCAAATCCTCCTAC+TGG 0.500963 8_1:+48260668 Msa1197600:CDS
TGCTACAATGCTGGAAACTA+CGG 0.508443 8_1:-48260968 None:intergenic
AGTGTTGGTGGTAGTGGTAG+TGG 0.513157 8_1:+48259134 Msa1197600:CDS
ATCGGCGGCTCCGCCGCCGG+TGG 0.513840 8_1:-48258499 None:intergenic
TGTGGATTTATGTTCGGTGA+AGG 0.514081 8_1:+48261018 Msa1197600:three_prime_UTR
TATTAGATAATATAACAAGA+TGG 0.517636 8_1:-48260933 None:intergenic
CGTCGCCCAGTCAGCTTGGT+CGG 0.519038 8_1:-48258532 None:intergenic
CACGATCTGATGCACATACA+AGG 0.519654 8_1:-48261096 None:intergenic
AAAGTACAACTATCTCCATA+AGG 0.520248 8_1:-48259002 None:intergenic
CAAATCATCACCACCGGCGG+CGG 0.520822 8_1:+48258489 Msa1197600:CDS
TCAGTAGGATATATGGCGAT+TGG 0.521816 8_1:+48260792 Msa1197600:CDS
ACAATCTCCTGCCAATTCTC+CGG 0.526020 8_1:-48258825 None:intergenic
CAATGGAAGGTCTTTGTGAA+GGG 0.529629 8_1:-48260715 None:intergenic
GGAGAATTGGCAGGAGATTG+TGG 0.530968 8_1:+48258827 Msa1197600:CDS
TCCACCACCACCATAACCAC+CGG 0.534604 8_1:-48259078 None:intergenic
TATTCCCAATCTTGGAAACA+TGG 0.539439 8_1:+48260846 Msa1197600:three_prime_UTR
TCGGCCGCCGGAGAATTGGC+AGG 0.541968 8_1:+48258818 Msa1197600:CDS
GGTCCCGGTGGTTATGGTGG+TGG 0.546890 8_1:+48259074 Msa1197600:CDS
AAAATGGCATTTGTTACCAA+AGG 0.546912 8_1:-48259267 None:intergenic
GACAGAGAGCTCATCAGAAG+TGG 0.547469 8_1:+48260753 Msa1197600:CDS
ATTAGATAATATAACAAGAT+GGG 0.550868 8_1:-48260932 None:intergenic
GTGGCGATTAGTTTAGGTCC+CGG 0.552008 8_1:+48259059 Msa1197600:CDS
GGTATCTGAAACTCCTCCCT+AGG 0.552310 8_1:-48258885 None:intergenic
GGCCTCTGACTCACAATGGA+AGG 0.553370 8_1:-48260728 None:intergenic
TCGAGAAGCAATTCGCAAGA+TGG 0.554276 8_1:+48258651 Msa1197600:CDS
GAGTGTGATTGAGCAGCCTA+GGG 0.557000 8_1:+48258869 Msa1197600:CDS
AAATGGCATTTGTTACCAAA+GGG 0.557541 8_1:-48259266 None:intergenic
GTAATAAGTGGGAGATGACT+GGG 0.557999 8_1:+48259237 Msa1197600:CDS
AATTCGCAAGATGGTGGTGG+TGG 0.565492 8_1:+48258660 Msa1197600:CDS
ATCTTAAATTAGATCCAACA+TGG 0.566982 8_1:-48261145 None:intergenic
TCCATTGTGAGTCAGAGGCC+AGG 0.568730 8_1:+48260731 Msa1197600:CDS
CTATGCTGTAAGTTTCGTGT+GGG 0.569773 8_1:+48258741 Msa1197600:CDS
TGGAAAATGGCTGATGATGA+GGG 0.572671 8_1:+48260866 Msa1197600:three_prime_UTR
ATTCACAGATAATTGTTCTG+TGG 0.573853 8_1:+48261000 Msa1197600:three_prime_UTR
GACCTTCCATTGTGAGTCAG+AGG 0.580448 8_1:+48260726 Msa1197600:CDS
ATGGAAAATGGCTGATGATG+AGG 0.582450 8_1:+48260865 Msa1197600:three_prime_UTR
TATGGAGGTGGGCTTATGGA+AGG 0.583879 8_1:+48260566 Msa1197600:CDS
CGAATCGCGCGAAAGCGATA+GGG 0.588275 8_1:+48258697 Msa1197600:CDS
CCACCACCACCATAACCACC+GGG 0.590124 8_1:-48259077 None:intergenic
AGCAATTCGCAAGATGGTGG+TGG 0.591086 8_1:+48258657 Msa1197600:CDS
GAATTACACAGATATGGAGG+TGG 0.593880 8_1:+48260554 Msa1197600:intron
ACAATGGAAGGTCTTTGTGA+AGG 0.593936 8_1:-48260716 None:intergenic
TCCTGGCCTCTGACTCACAA+TGG 0.594508 8_1:-48260732 None:intergenic
TGTTGTTGTTGCAGCTGGTG+CGG 0.594830 8_1:+48259160 Msa1197600:CDS
CGACCAAGCTGACTGGGCGA+CGG 0.595148 8_1:+48258533 Msa1197600:CDS
TTGGTCGGTTGACCAGCTAT+CGG 0.595738 8_1:-48258517 None:intergenic
GATGAGTATCGTTACTGGAA+CGG 0.598603 8_1:+48258558 Msa1197600:CDS
CAAAACTCATGCTGATAATG+TGG 0.598802 8_1:+48260646 Msa1197600:CDS
GTCGTTCAAATCATCACCAC+CGG 0.600321 8_1:+48258483 Msa1197600:CDS
AGAGCTCATCAGAAGTGGAA+AGG 0.606297 8_1:+48260758 Msa1197600:CDS
TTAGGTCCCGGTGGTTATGG+TGG 0.607828 8_1:+48259071 Msa1197600:CDS
GAGTTTCAGATACCGACGGT+TGG 0.612303 8_1:+48258894 Msa1197600:CDS
AAAGTACTCGACCCAACCGT+CGG 0.614579 8_1:-48258906 None:intergenic
ACTATGCTGTAAGTTTCGTG+TGG 0.615988 8_1:+48258740 Msa1197600:CDS
ATCGAAGAATCGTGAAAGTG+TGG 0.618402 8_1:+48259040 Msa1197600:CDS
TCTTAAATTAGATCCAACAT+GGG 0.621380 8_1:-48261144 None:intergenic
AAGGTCCCGATAAAATCAGT+AGG 0.622761 8_1:+48260777 Msa1197600:CDS
GGAGGAGTTTCAGATACCGA+CGG 0.623970 8_1:+48258890 Msa1197600:CDS
TGTGATTGAGCAGCCTAGGG+AGG 0.629299 8_1:+48258872 Msa1197600:CDS
ATAGTGTGGAGGAGCTGCGT+CGG 0.631733 8_1:+48258799 Msa1197600:CDS
TAAACATCTGAACCAGTAGG+AGG 0.634383 8_1:-48260680 None:intergenic
AATTACACAGATATGGAGGT+GGG 0.635355 8_1:+48260555 Msa1197600:CDS
AGAAGCAATTCGCAAGATGG+TGG 0.638549 8_1:+48258654 Msa1197600:CDS
TGATAAACATCTGAACCAGT+AGG 0.638955 8_1:-48260683 None:intergenic
TGAATAATAGGTCTTATAAG+GGG 0.644881 8_1:+48258943 Msa1197600:CDS
CCTGCACGCGAATTTGAAGA+CGG 0.647659 8_1:-48258606 None:intergenic
TGGCTGCTCATGAAGAAGAG+AGG 0.650335 8_1:+48258847 Msa1197600:CDS
ACAATGGATTACAACAGAGA+CGG 0.651622 8_1:+48258456 None:intergenic
ATGGAAGGAGAAAGCAGAGA+CGG 0.651866 8_1:+48260581 Msa1197600:CDS
ATCTCCTGCCAATTCTCCGG+CGG 0.654680 8_1:-48258822 None:intergenic
GTTCAAATCATCACCACCGG+CGG 0.671677 8_1:+48258486 Msa1197600:CDS
GTCGGTTGACCAGCTATCGG+CGG 0.673668 8_1:-48258514 None:intergenic
GCAGAATTACACAGATATGG+AGG 0.676863 8_1:+48260551 Msa1197600:intron
CTTAAATTAGATCCAACATG+GGG 0.685023 8_1:-48261143 None:intergenic
TGGAACGGAAACGATGCTGG+TGG 0.699268 8_1:+48258573 Msa1197600:CDS
ATTAGGTCCATTCCCAACCG+CGG 0.701157 8_1:-48259183 None:intergenic
CTTCTCGATTTCTTACTCGG+CGG 0.702870 8_1:-48258636 None:intergenic
GCGATTAGTTTAGGTCCCGG+TGG 0.706931 8_1:+48259062 Msa1197600:CDS
TATGCTGTAAGTTTCGTGTG+GGG 0.708514 8_1:+48258742 Msa1197600:CDS
GTTGCGTCTGACACAAAACA+GGG 0.785414 8_1:+48260614 Msa1197600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTAAAGATTTTAATTTTA+TGG + chr8_1:48259347-48259366 Msa1197600:intron 10.0%
!!! TTTTAATTTTATGGTAATTG+AGG + chr8_1:48259356-48259375 Msa1197600:intron 15.0%
!! ATTAGATAATATAACAAGAT+GGG - chr8_1:48260935-48260954 None:intergenic 15.0%
!! TATTAGATAATATAACAAGA+TGG - chr8_1:48260936-48260955 None:intergenic 15.0%
!! AAATTACAGTTCGTTATATA+AGG - chr8_1:48258774-48258793 None:intergenic 20.0%
!!! TATGAATAATAGGTCTTATA+AGG + chr8_1:48258941-48258960 Msa1197600:CDS 20.0%
!!! ATGAATAATAGGTCTTATAA+GGG + chr8_1:48258942-48258961 Msa1197600:CDS 20.0%
!!! AAGAAATTTTATACTGAAGA+AGG + chr8_1:48258975-48258994 Msa1197600:CDS 20.0%
!! ACATAACAAAATCATCAAAA+TGG - chr8_1:48259321-48259340 None:intergenic 20.0%
!!! GGTTTTTTCTTCATTTAATA+TGG + chr8_1:48259529-48259548 Msa1197600:intron 20.0%
!!! ATATATCCTACTGATTTTAT+CGG - chr8_1:48260786-48260805 None:intergenic 20.0%
!! TGAATAATAGGTCTTATAAG+GGG + chr8_1:48258943-48258962 Msa1197600:CDS 25.0%
! AAACTAGGATTTGTAATAAG+TGG + chr8_1:48259225-48259244 Msa1197600:CDS 25.0%
! AACTAGGATTTGTAATAAGT+GGG + chr8_1:48259226-48259245 Msa1197600:CDS 25.0%
!!! GATGATTTTGTTATGTGAAT+TGG + chr8_1:48259325-48259344 Msa1197600:intron 25.0%
!!! ATAGAAAGTGCTATTAGAAT+TGG + chr8_1:48259402-48259421 Msa1197600:intron 25.0%
!! GTTATAAATAGTTTGTGTTG+TGG + chr8_1:48259428-48259447 Msa1197600:intron 25.0%
!! TTACCATTTGGATACAATTT+TGG + chr8_1:48259469-48259488 Msa1197600:intron 25.0%
!! TACCATTTGGATACAATTTT+GGG + chr8_1:48259470-48259489 Msa1197600:intron 25.0%
!! CTTTTTAATCCATACAAGAT+TGG + chr8_1:48259508-48259527 Msa1197600:intron 25.0%
! AAGAAAAAACCAATCTTGTA+TGG - chr8_1:48259520-48259539 None:intergenic 25.0%
!! TTTTGATATGTATGTGAATG+TGG + chr8_1:48259652-48259671 Msa1197600:intron 25.0%
!! TTTTCATTGTGCAAAACATA+AGG - chr8_1:48259797-48259816 None:intergenic 25.0%
! ATGAAAAGTAATCGCAAAAA+AGG - chr8_1:48259905-48259924 None:intergenic 25.0%
!! ATTTGAATTTGAAGCATACA+AGG - chr8_1:48259931-48259950 None:intergenic 25.0%
!! TTTTCTTAATCAGAAAGTGA+AGG - chr8_1:48259961-48259980 None:intergenic 25.0%
! AAAATGTATATGTGCATGTT+TGG - chr8_1:48260412-48260431 None:intergenic 25.0%
!!! TATATCCTACTGATTTTATC+GGG - chr8_1:48260785-48260804 None:intergenic 25.0%
! GATAAAATCAGTAGGATATA+TGG + chr8_1:48260785-48260804 Msa1197600:CDS 25.0%
! CAATATTATATTCCCAATCT+TGG + chr8_1:48260838-48260857 Msa1197600:three_prime_UTR 25.0%
! TCTTAAATTAGATCCAACAT+GGG - chr8_1:48261147-48261166 None:intergenic 25.0%
! ATCTTAAATTAGATCCAACA+TGG - chr8_1:48261148-48261167 None:intergenic 25.0%
!! TTGGATCTAATTTAAGATTC+CGG + chr8_1:48261150-48261169 Msa1197600:three_prime_UTR 25.0%
AAAGTACAACTATCTCCATA+AGG - chr8_1:48259005-48259024 None:intergenic 30.0%
! AAATGGCATTTGTTACCAAA+GGG - chr8_1:48259269-48259288 None:intergenic 30.0%
! AAAATGGCATTTGTTACCAA+AGG - chr8_1:48259270-48259289 None:intergenic 30.0%
TTCCCAAAATTGTATCCAAA+TGG - chr8_1:48259475-48259494 None:intergenic 30.0%
CCAAAACAAAATTGACACTA+GGG - chr8_1:48259608-48259627 None:intergenic 30.0%
ACCAAAACAAAATTGACACT+AGG - chr8_1:48259609-48259628 None:intergenic 30.0%
!!! TTTTGTTTTGGTTGATGCTA+AGG + chr8_1:48259617-48259636 Msa1197600:intron 30.0%
ATTGCATAGATTACTCAAGT+CGG - chr8_1:48259713-48259732 None:intergenic 30.0%
!!! GCAAATTTCCAAGTGTTTTT+TGG + chr8_1:48259867-48259886 Msa1197600:intron 30.0%
ACTGTAAACCAAAAAACACT+TGG - chr8_1:48259878-48259897 None:intergenic 30.0%
GAAAAGAATAGTTTCATGAG+TGG + chr8_1:48260001-48260020 Msa1197600:intron 30.0%
ACTTTCATTGCTTTGATGTT+TGG + chr8_1:48260240-48260259 Msa1197600:intron 30.0%
AAACATGCACATAATGAACA+AGG - chr8_1:48260266-48260285 None:intergenic 30.0%
TTGTTCATTATGTGCATGTT+TGG + chr8_1:48260265-48260284 Msa1197600:intron 30.0%
! TTGATACACGAGTGTTTTAT+TGG + chr8_1:48260450-48260469 Msa1197600:intron 30.0%
GGCTTAAATTGATTATCTGT+CGG + chr8_1:48260471-48260490 Msa1197600:intron 30.0%
! TTTTCCATGTTTCCAAGATT+GGG - chr8_1:48260853-48260872 None:intergenic 30.0%
! ATTTTCCATGTTTCCAAGAT+TGG - chr8_1:48260854-48260873 None:intergenic 30.0%
AATCTTGGAAACATGGAAAA+TGG + chr8_1:48260853-48260872 Msa1197600:three_prime_UTR 30.0%
TAATATAACAAGATGGGAAC+TGG - chr8_1:48260929-48260948 None:intergenic 30.0%
ATTCACAGATAATTGTTCTG+TGG + chr8_1:48261000-48261019 Msa1197600:three_prime_UTR 30.0%
TTGTTCTGTGGATTTATGTT+CGG + chr8_1:48261012-48261031 Msa1197600:three_prime_UTR 30.0%
CTTAAATTAGATCCAACATG+GGG - chr8_1:48261146-48261165 None:intergenic 30.0%
TTGCTTCTCGATTTCTTACT+CGG - chr8_1:48258642-48258661 None:intergenic 35.0%
! CTGTAATTTTGCTCATAGTG+TGG + chr8_1:48258785-48258804 Msa1197600:CDS 35.0%
! TAATTTTGCTCATAGTGTGG+AGG + chr8_1:48258788-48258807 Msa1197600:CDS 35.0%
! TTGGGGAGAGTATGAATAAT+AGG + chr8_1:48258931-48258950 Msa1197600:CDS 35.0%
CAATTCGAAGGCTTCAAATT+AGG - chr8_1:48259203-48259222 None:intergenic 35.0%
! CTAATTTGAAGCCTTCGAAT+TGG + chr8_1:48259201-48259220 Msa1197600:CDS 35.0%
!! ATCCTAGTTTTCCAATTCGA+AGG - chr8_1:48259215-48259234 None:intergenic 35.0%
!!! AACAAATGCCATTTTGCTCA+TGG + chr8_1:48259275-48259294 Msa1197600:CDS 35.0%
!! CCCTAGTGTCAATTTTGTTT+TGG + chr8_1:48259605-48259624 Msa1197600:intron 35.0%
CATTGTGCAAAACATAAGGA+AGG - chr8_1:48259793-48259812 None:intergenic 35.0%
AAGAATAGTTTCATGAGTGG+TGG + chr8_1:48260004-48260023 Msa1197600:intron 35.0%
AGAATAGTTTCATGAGTGGT+GGG + chr8_1:48260005-48260024 Msa1197600:intron 35.0%
TGTACTTAATCGCATATTCG+TGG - chr8_1:48260129-48260148 None:intergenic 35.0%
TGTTGTCAAATAGCGTCTAT+AGG + chr8_1:48260152-48260171 Msa1197600:intron 35.0%
TATGTGCATGTTTGGATTGA+CGG + chr8_1:48260273-48260292 Msa1197600:intron 35.0%
ACTGCAGAATTACACAGATA+TGG + chr8_1:48260548-48260567 Msa1197600:intron 35.0%
AATTACACAGATATGGAGGT+GGG + chr8_1:48260555-48260574 Msa1197600:CDS 35.0%
!!! TTATCAGCATGAGTTTTGGA+AGG - chr8_1:48260644-48260663 None:intergenic 35.0%
! CACATTATCAGCATGAGTTT+TGG - chr8_1:48260648-48260667 None:intergenic 35.0%
! CAAAACTCATGCTGATAATG+TGG + chr8_1:48260646-48260665 Msa1197600:CDS 35.0%
TGATAAACATCTGAACCAGT+AGG - chr8_1:48260686-48260705 None:intergenic 35.0%
GGTTCAGATGTTTATCATCT+TGG + chr8_1:48260689-48260708 Msa1197600:CDS 35.0%
TATTCCCAATCTTGGAAACA+TGG + chr8_1:48260846-48260865 Msa1197600:three_prime_UTR 35.0%
!!! CTGAGTTTTGAATGTAACAG+CGG + chr8_1:48261070-48261089 Msa1197600:three_prime_UTR 35.0%
! GATGAGTATCGTTACTGGAA+CGG + chr8_1:48258558-48258577 Msa1197600:CDS 40.0%
ACTATGCTGTAAGTTTCGTG+TGG + chr8_1:48258740-48258759 Msa1197600:CDS 40.0%
CTATGCTGTAAGTTTCGTGT+GGG + chr8_1:48258741-48258760 Msa1197600:CDS 40.0%
TATGCTGTAAGTTTCGTGTG+GGG + chr8_1:48258742-48258761 Msa1197600:CDS 40.0%
! TTGGGTCGAGTACTTTTGTT+GGG + chr8_1:48258913-48258932 Msa1197600:CDS 40.0%
ACTGAAGAAGGTTGTCCTTA+TGG + chr8_1:48258987-48259006 Msa1197600:CDS 40.0%
ATCGAAGAATCGTGAAAGTG+TGG + chr8_1:48259040-48259059 Msa1197600:CDS 40.0%
!! GAAAGTGTGGCGATTAGTTT+AGG + chr8_1:48259053-48259072 Msa1197600:CDS 40.0%
AGCCTTCGAATTGGAAAACT+AGG + chr8_1:48259210-48259229 Msa1197600:CDS 40.0%
TGTAATAAGTGGGAGATGAC+TGG + chr8_1:48259236-48259255 Msa1197600:CDS 40.0%
GTAATAAGTGGGAGATGACT+GGG + chr8_1:48259237-48259256 Msa1197600:CDS 40.0%
! TAGTTTGTGTTGTGGAGTTG+TGG + chr8_1:48259436-48259455 Msa1197600:intron 40.0%
! GGCAGTGTTTTCTTACCATT+TGG + chr8_1:48259457-48259476 Msa1197600:intron 40.0%
! CTCTTTTTGTGATCCGCAAT+CGG + chr8_1:48260211-48260230 Msa1197600:intron 40.0%
! TGAAAGTATGTTGCCGATTG+CGG - chr8_1:48260227-48260246 None:intergenic 40.0%
AGACTGGCATGACATAATCA+TGG - chr8_1:48260379-48260398 None:intergenic 40.0%
TATGTGCATGTTTGGATCGA+CGG - chr8_1:48260404-48260423 None:intergenic 40.0%
GCAGAATTACACAGATATGG+AGG + chr8_1:48260551-48260570 Msa1197600:intron 40.0%
GAATTACACAGATATGGAGG+TGG + chr8_1:48260554-48260573 Msa1197600:intron 40.0%
TAAACATCTGAACCAGTAGG+AGG - chr8_1:48260683-48260702 None:intergenic 40.0%
CAATGGAAGGTCTTTGTGAA+GGG - chr8_1:48260718-48260737 None:intergenic 40.0%
ACAATGGAAGGTCTTTGTGA+AGG - chr8_1:48260719-48260738 None:intergenic 40.0%
AAGGTCCCGATAAAATCAGT+AGG + chr8_1:48260777-48260796 Msa1197600:CDS 40.0%
TCAGTAGGATATATGGCGAT+TGG + chr8_1:48260792-48260811 Msa1197600:CDS 40.0%
! ATGGAAAATGGCTGATGATG+AGG + chr8_1:48260865-48260884 Msa1197600:three_prime_UTR 40.0%
! TGGAAAATGGCTGATGATGA+GGG + chr8_1:48260866-48260885 Msa1197600:three_prime_UTR 40.0%
! TCATGACGTTTTCAGCTACA+TGG - chr8_1:48260903-48260922 None:intergenic 40.0%
TGCTACAATGCTGGAAACTA+CGG - chr8_1:48260971-48260990 None:intergenic 40.0%
! TGAACAAAGTGCTACAATGC+TGG - chr8_1:48260980-48260999 None:intergenic 40.0%
! TGTGGATTTATGTTCGGTGA+AGG + chr8_1:48261018-48261037 Msa1197600:three_prime_UTR 40.0%
! GTTCTTTTGTTACCCCATGT+TGG + chr8_1:48261131-48261150 Msa1197600:three_prime_UTR 40.0%
GTCGTTCAAATCATCACCAC+CGG + chr8_1:48258483-48258502 Msa1197600:CDS 45.0%
! CGGAAGATGAGTATCGTTAC+TGG + chr8_1:48258553-48258572 Msa1197600:CDS 45.0%
CTTCTCGATTTCTTACTCGG+CGG - chr8_1:48258639-48258658 None:intergenic 45.0%
TCGAGAAGCAATTCGCAAGA+TGG + chr8_1:48258651-48258670 Msa1197600:CDS 45.0%
AGAAGCAATTCGCAAGATGG+TGG + chr8_1:48258654-48258673 Msa1197600:CDS 45.0%
ACAATCTCCTGCCAATTCTC+CGG - chr8_1:48258828-48258847 None:intergenic 45.0%
AGTTTCAGATACCGACGGTT+GGG + chr8_1:48258895-48258914 Msa1197600:CDS 45.0%
! GTTGGGTCGAGTACTTTTGT+TGG + chr8_1:48258912-48258931 Msa1197600:CDS 45.0%
! TGGGTCGAGTACTTTTGTTG+GGG + chr8_1:48258914-48258933 Msa1197600:CDS 45.0%
! ATGACTGGGTATTGTCCCTT+TGG + chr8_1:48259251-48259270 Msa1197600:CDS 45.0%
CTGTAGCACCATGAGCAAAA+TGG - chr8_1:48259286-48259305 None:intergenic 45.0%
!!! CATTTTGCTCATGGTGCTAC+AGG + chr8_1:48259284-48259303 Msa1197600:CDS 45.0%
TTGACACTAGGGACAATGCA+GGG - chr8_1:48259597-48259616 None:intergenic 45.0%
ATTGACACTAGGGACAATGC+AGG - chr8_1:48259598-48259617 None:intergenic 45.0%
! GGTGGGTTCAGAATGAAAGA+TGG + chr8_1:48260022-48260041 Msa1197600:intron 45.0%
TAGGATAGCACAGTACGTAG+TGG + chr8_1:48260171-48260190 Msa1197600:intron 45.0%
ATGGAAGGAGAAAGCAGAGA+CGG + chr8_1:48260581-48260600 Msa1197600:CDS 45.0%
TGTTGCGTCTGACACAAAAC+AGG + chr8_1:48260613-48260632 Msa1197600:CDS 45.0%
GTTGCGTCTGACACAAAACA+GGG + chr8_1:48260614-48260633 Msa1197600:CDS 45.0%
GTTGCATCAAATCCTCCTAC+TGG + chr8_1:48260668-48260687 Msa1197600:CDS 45.0%
AGAGCTCATCAGAAGTGGAA+AGG + chr8_1:48260758-48260777 Msa1197600:CDS 45.0%
CACGATCTGATGCACATACA+AGG - chr8_1:48261099-48261118 None:intergenic 45.0%
GTTCAAATCATCACCACCGG+CGG + chr8_1:48258486-48258505 Msa1197600:CDS 50.0%
TTGGTCGGTTGACCAGCTAT+CGG - chr8_1:48258520-48258539 None:intergenic 50.0%
TACTGGAACGGAAACGATGC+TGG + chr8_1:48258570-48258589 Msa1197600:CDS 50.0%
CCTGCACGCGAATTTGAAGA+CGG - chr8_1:48258609-48258628 None:intergenic 50.0%
! AGCAATTCGCAAGATGGTGG+TGG + chr8_1:48258657-48258676 Msa1197600:CDS 50.0%
!! AATTCGCAAGATGGTGGTGG+TGG + chr8_1:48258660-48258679 Msa1197600:CDS 50.0%
GGAGAATTGGCAGGAGATTG+TGG + chr8_1:48258827-48258846 Msa1197600:CDS 50.0%
TGGCTGCTCATGAAGAAGAG+AGG + chr8_1:48258847-48258866 Msa1197600:CDS 50.0%
GAGTGTGATTGAGCAGCCTA+GGG + chr8_1:48258869-48258888 Msa1197600:CDS 50.0%
! GGTATCTGAAACTCCTCCCT+AGG - chr8_1:48258888-48258907 None:intergenic 50.0%
GGAGGAGTTTCAGATACCGA+CGG + chr8_1:48258890-48258909 Msa1197600:CDS 50.0%
GAGTTTCAGATACCGACGGT+TGG + chr8_1:48258894-48258913 Msa1197600:CDS 50.0%
! AAAGTACTCGACCCAACCGT+CGG - chr8_1:48258909-48258928 None:intergenic 50.0%
!! GTGGCGATTAGTTTAGGTCC+CGG + chr8_1:48259059-48259078 Msa1197600:CDS 50.0%
AGTTTAGGTCCCGGTGGTTA+TGG + chr8_1:48259068-48259087 Msa1197600:CDS 50.0%
!! GTTGGTAGTGTTGGTGGTAG+TGG + chr8_1:48259128-48259147 Msa1197600:CDS 50.0%
!! AGTGTTGGTGGTAGTGGTAG+TGG + chr8_1:48259134-48259153 Msa1197600:CDS 50.0%
!! GGTGTTGTTGTTGTTGCAGC+TGG + chr8_1:48259155-48259174 Msa1197600:CDS 50.0%
! TGTTGTTGTTGCAGCTGGTG+CGG + chr8_1:48259160-48259179 Msa1197600:CDS 50.0%
ATTAGGTCCATTCCCAACCG+CGG - chr8_1:48259186-48259205 None:intergenic 50.0%
! CAGATATGGAGGTGGGCTTA+TGG + chr8_1:48260562-48260581 Msa1197600:CDS 50.0%
! TATGGAGGTGGGCTTATGGA+AGG + chr8_1:48260566-48260585 Msa1197600:CDS 50.0%
GACCTTCCATTGTGAGTCAG+AGG + chr8_1:48260726-48260745 Msa1197600:CDS 50.0%
TTCTGATGAGCTCTCTGTCC+TGG - chr8_1:48260752-48260771 None:intergenic 50.0%
GACAGAGAGCTCATCAGAAG+TGG + chr8_1:48260753-48260772 Msa1197600:CDS 50.0%
GTCAACCGACCAAGCTGACT+GGG + chr8_1:48258527-48258546 Msa1197600:CDS 55.0%
TGGAACGGAAACGATGCTGG+TGG + chr8_1:48258573-48258592 Msa1197600:CDS 55.0%
CCGTCTTCAAATTCGCGTGC+AGG + chr8_1:48258606-48258625 Msa1197600:CDS 55.0%
TCGAATCGCGCGAAAGCGAT+AGG + chr8_1:48258696-48258715 Msa1197600:CDS 55.0%
CGAATCGCGCGAAAGCGATA+GGG + chr8_1:48258697-48258716 Msa1197600:CDS 55.0%
ATAGTGTGGAGGAGCTGCGT+CGG + chr8_1:48258799-48258818 Msa1197600:CDS 55.0%
ATCTCCTGCCAATTCTCCGG+CGG - chr8_1:48258825-48258844 None:intergenic 55.0%
GGAGTGTGATTGAGCAGCCT+AGG + chr8_1:48258868-48258887 Msa1197600:CDS 55.0%
TGTGATTGAGCAGCCTAGGG+AGG + chr8_1:48258872-48258891 Msa1197600:CDS 55.0%
!! GCGATTAGTTTAGGTCCCGG+TGG + chr8_1:48259062-48259081 Msa1197600:CDS 55.0%
TTAGGTCCCGGTGGTTATGG+TGG + chr8_1:48259071-48259090 Msa1197600:CDS 55.0%
TCCACCACCACCATAACCAC+CGG - chr8_1:48259081-48259100 None:intergenic 55.0%
!! GGTGGGAGTGTTGGTAGTGT+TGG + chr8_1:48259119-48259138 Msa1197600:CDS 55.0%
!! GGGAGTGTTGGTAGTGTTGG+TGG + chr8_1:48259122-48259141 Msa1197600:CDS 55.0%
AGTGCTACAGCGCTATCGTG+TGG + chr8_1:48260077-48260096 Msa1197600:intron 55.0%
CGGCGAGTTTAGCGAAATCG+CGG + chr8_1:48260293-48260312 Msa1197600:intron 55.0%
CGAGTTTAGCGAAATCGCGG+TGG + chr8_1:48260296-48260315 Msa1197600:intron 55.0%
GTTTGGATCGACGGTGAGAC+TGG - chr8_1:48260395-48260414 None:intergenic 55.0%
GGCCTCTGACTCACAATGGA+AGG - chr8_1:48260731-48260750 None:intergenic 55.0%
TCCATTGTGAGTCAGAGGCC+AGG + chr8_1:48260731-48260750 Msa1197600:CDS 55.0%
TCCTGGCCTCTGACTCACAA+TGG - chr8_1:48260735-48260754 None:intergenic 55.0%
CAAATCATCACCACCGGCGG+CGG + chr8_1:48258489-48258508 Msa1197600:CDS 60.0%
GTCGGTTGACCAGCTATCGG+CGG - chr8_1:48258517-48258536 None:intergenic 60.0%
GGTCAACCGACCAAGCTGAC+TGG + chr8_1:48258526-48258545 Msa1197600:CDS 60.0%
!! CAAGATGGTGGTGGTGGCAG+TGG + chr8_1:48258666-48258685 Msa1197600:CDS 60.0%
CCACCACCACCATAACCACC+GGG - chr8_1:48259080-48259099 None:intergenic 60.0%
!! GGTGGTTATGGTGGTGGTGG+AGG + chr8_1:48259080-48259099 Msa1197600:CDS 60.0%
!! GGTTATGGTGGTGGTGGAGG+TGG + chr8_1:48259083-48259102 Msa1197600:CDS 60.0%
!! TATGGTGGTGGTGGAGGTGG+TGG + chr8_1:48259086-48259105 Msa1197600:CDS 60.0%
CGTCGCCCAGTCAGCTTGGT+CGG - chr8_1:48258535-48258554 None:intergenic 65.0%
CGACCAAGCTGACTGGGCGA+CGG + chr8_1:48258533-48258552 Msa1197600:CDS 65.0%
CTTCCGTCGCCCAGTCAGCT+TGG - chr8_1:48258539-48258558 None:intergenic 65.0%
!! GATGGTGGTGGTGGCAGTGG+AGG + chr8_1:48258669-48258688 Msa1197600:CDS 65.0%
GGTCCCGGTGGTTATGGTGG+TGG + chr8_1:48259074-48259093 Msa1197600:CDS 65.0%
!! CCCGGTGGTTATGGTGGTGG+TGG + chr8_1:48259077-48259096 Msa1197600:CDS 65.0%
! GTGGTGGAGGAGGAGGAGGT+GGG + chr8_1:48259102-48259121 Msa1197600:CDS 65.0%
! GGAGGAGGAGGTGGGAGTGT+TGG + chr8_1:48259110-48259129 Msa1197600:CDS 65.0%
!! GGTGGTGGTGGCAGTGGAGG+AGG + chr8_1:48258672-48258691 Msa1197600:CDS 70.0%
! TGCGTCGGCCGCCGGAGAAT+TGG + chr8_1:48258814-48258833 Msa1197600:CDS 70.0%
TCGGCCGCCGGAGAATTGGC+AGG + chr8_1:48258818-48258837 Msa1197600:CDS 70.0%
!! GGTGGTGGTGGAGGTGGTGG+AGG + chr8_1:48259089-48259108 Msa1197600:CDS 70.0%
!! GGTGGTGGAGGTGGTGGAGG+AGG + chr8_1:48259092-48259111 Msa1197600:CDS 70.0%
!! GGTGGAGGTGGTGGAGGAGG+AGG + chr8_1:48259095-48259114 Msa1197600:CDS 70.0%
!! GGAGGTGGTGGAGGAGGAGG+AGG + chr8_1:48259098-48259117 Msa1197600:CDS 70.0%
!! GGTGGTGGAGGAGGAGGAGG+TGG + chr8_1:48259101-48259120 Msa1197600:CDS 70.0%
TGTTGCAGCTGGTGCGGCCG+CGG + chr8_1:48259166-48259185 Msa1197600:CDS 70.0%
CAGCTGGTGCGGCCGCGGTT+GGG + chr8_1:48259171-48259190 Msa1197600:CDS 75.0%
GGTGCGGCCGCGGTTGGGAA+TGG + chr8_1:48259176-48259195 Msa1197600:CDS 75.0%
! GCTATCGGCGGCTCCGCCGC+CGG - chr8_1:48258505-48258524 None:intergenic 80.0%
! GCGGCGGAGCCGCCGATAGC+TGG + chr8_1:48258505-48258524 Msa1197600:CDS 80.0%
!! GGAGGAGCTGCGTCGGCCGC+CGG + chr8_1:48258806-48258825 Msa1197600:CDS 80.0%
! GCAGCTGGTGCGGCCGCGGT+TGG + chr8_1:48259170-48259189 Msa1197600:CDS 80.0%
! ATCGGCGGCTCCGCCGCCGG+TGG - chr8_1:48258502-48258521 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 48258459 48261183 48258459 ID=Msa1197600;Name=Msa1197600
chr8_1 mRNA 48258459 48261183 48258459 ID=Msa1197600-mRNA-1;Parent=Msa1197600;Name=Msa1197600-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|351|374
chr8_1 exon 48258459 48259305 48258459 ID=Msa1197600-mRNA-1:exon:15223;Parent=Msa1197600-mRNA-1
chr8_1 exon 48260555 48261183 48260555 ID=Msa1197600-mRNA-1:exon:15224;Parent=Msa1197600-mRNA-1
chr8_1 CDS 48258459 48259305 48258459 ID=Msa1197600-mRNA-1:cds;Parent=Msa1197600-mRNA-1
chr8_1 CDS 48260555 48260832 48260555 ID=Msa1197600-mRNA-1:cds;Parent=Msa1197600-mRNA-1
chr8_1 three_prime_UTR 48260833 48261183 48260833 ID=Msa1197600-mRNA-1:three_prime_utr;Parent=Msa1197600-mRNA-1
Gene Sequence

>Msa1197600

ATGGATTACAACAGAGACGGCAACGTCGTTCAAATCATCACCACCGGCGGCGGAGCCGCCGATAGCTGGTCAACCGACCAAGCTGACTGGGCGACGGAAGATGAGTATCGTTACTGGAACGGAAACGATGCTGGTGGCGAAACAACTCCGTCTTCAAATTCGCGTGCAGGAAGTGAGCCGCCGAGTAAGAAATCGAGAAGCAATTCGCAAGATGGTGGTGGTGGCAGTGGAGGAGGATCGAATCGCGCGAAAGCGATAGGGAAAATGTTTTTTAAGACGAAACTATGCTGTAAGTTTCGTGTGGGGACATGTCCTTATATAACGAACTGTAATTTTGCTCATAGTGTGGAGGAGCTGCGTCGGCCGCCGGAGAATTGGCAGGAGATTGTGGCTGCTCATGAAGAAGAGAGGAGTGTGATTGAGCAGCCTAGGGAGGAGTTTCAGATACCGACGGTTGGGTCGAGTACTTTTGTTGGGGAGAGTATGAATAATAGGTCTTATAAGGGGAGACATTGTAAGAAATTTTATACTGAAGAAGGTTGTCCTTATGGAGATAGTTGTACTTTTCTTCATGATGAGCAATCGAAGAATCGTGAAAGTGTGGCGATTAGTTTAGGTCCCGGTGGTTATGGTGGTGGTGGAGGTGGTGGAGGAGGAGGAGGTGGGAGTGTTGGTAGTGTTGGTGGTAGTGGTAGTGGTGTTGTTGTTGTTGCAGCTGGTGCGGCCGCGGTTGGGAATGGACCTAATTTGAAGCCTTCGAATTGGAAAACTAGGATTTGTAATAAGTGGGAGATGACTGGGTATTGTCCCTTTGGTAACAAATGCCATTTTGCTCATGGTGCTACAGAATTACACAGATATGGAGGTGGGCTTATGGAAGGAGAAAGCAGAGACGGTGTTTCTGTTGTTGCGTCTGACACAAAACAGGGTGTACCTTCCAAAACTCATGCTGATAATGTGGTTGCATCAAATCCTCCTACTGGTTCAGATGTTTATCATCTTGGAGTCCCTTCACAAAGACCTTCCATTGTGAGTCAGAGGCCAGGACAGAGAGCTCATCAGAAGTGGAAAGGTCCCGATAAAATCAGTAGGATATATGGCGATTGGATCGATGACTTCGAATAG
Protein sequence

>Msa1197600

MDYNRDGNVVQIITTGGGAADSWSTDQADWATEDEYRYWNGNDAGGETTPSSNSRAGSEPPSKKSRSNSQDGGGGSGGGSNRAKAIGKMFFKTKLCCKFRVGTCPYITNCNFAHSVEELRRPPENWQEIVAAHEEERSVIEQPREEFQIPTVGSSTFVGESMNNRSYKGRHCKKFYTEEGCPYGDSCTFLHDEQSKNRESVAISLGPGGYGGGGGGGGGGGGSVGSVGGSGSGVVVVAAGAAAVGNGPNLKPSNWKTRICNKWEMTGYCPFGNKCHFAHGATELHRYGGGLMEGESRDGVSVVASDTKQGVPSKTHADNVVASNPPTGSDVYHLGVPSQRPSIVSQRPGQRAHQKWKGPDKISRIYGDWIDDFE*