AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1334750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334750 MtrunA17_Chr8g0366501 98.670 376 3 2 1 374 1 376 0.0 750
Msa1334750 MtrunA17_Chr4g0063401 37.624 202 89 6 89 283 77 248 1.79e-34 127
Msa1334750 MtrunA17_Chr4g0063361 33.333 201 97 5 80 280 76 239 1.92e-27 108
Msa1334750 MtrunA17_Chr4g0063391 28.405 257 115 5 35 284 16 210 1.11e-25 103
Msa1334750 MtrunA17_Chr3g0098251 28.571 224 105 5 60 280 4 175 1.47e-21 91.7
Msa1334750 MtrunA17_Chr4g0063371 36.975 119 46 2 80 198 56 145 9.16e-17 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1334750 AT1G32360.1 60.560 393 122 12 3 374 4 384 4.12e-132 383
Msa1334750 AT3G19360.1 36.755 302 154 10 84 373 98 374 7.08e-46 162
Msa1334750 AT3G19360.2 36.755 302 154 10 84 373 98 374 7.08e-46 162
Msa1334750 AT2G35430.1 41.739 230 63 9 167 374 72 252 1.83e-41 146
Msa1334750 AT2G35430.1 37.427 171 69 7 26 191 31 168 4.13e-20 89.0

Find 143 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGGAGGAGGAGGAGG+TGG 0.204425 8_4:+55275084 Msa1334750:CDS
GGTGGAGGTGGTGGAGGAGG+AGG 0.223597 8_4:+55275078 Msa1334750:CDS
GGAGGTGGTGGAGGAGGAGG+AGG 0.235103 8_4:+55275081 Msa1334750:CDS
TATGAATAATAGGTCTTATA+AGG 0.235365 8_4:+55274924 Msa1334750:CDS
ACTGAAGAAGGTTGTCCTTA+TGG 0.260800 8_4:+55274970 Msa1334750:CDS
GGTGGTGGAGGTGGTGGAGG+AGG 0.265178 8_4:+55275075 Msa1334750:CDS
TATGGTGGTGGTGGAGGTGG+TGG 0.279719 8_4:+55275069 Msa1334750:CDS
CACATTATCAGCATGAGTTT+TGG 0.299688 8_4:-55276628 None:intergenic
GGTGGTGGTGGAGGTGGTGG+AGG 0.301864 8_4:+55275072 Msa1334750:CDS
CAATATTATATTCCCAATCT+TGG 0.304856 8_4:+55276821 Msa1334750:three_prime_UTR
CAATTCGAAGGCTTCAAATT+AGG 0.308899 8_4:-55275183 None:intergenic
AATCTTGGAAACATGGAAAA+TGG 0.310585 8_4:+55276836 Msa1334750:three_prime_UTR
AAATTACAGTTCGTTATATA+AGG 0.316371 8_4:-55274754 None:intergenic
CAGATATGGAGGTGGGCTTA+TGG 0.316746 8_4:+55276545 Msa1334750:CDS
GGTTCAGATGTTTATCATCT+TGG 0.329111 8_4:+55276672 Msa1334750:CDS
AGTTTAGGTCCCGGTGGTTA+TGG 0.330311 8_4:+55275051 Msa1334750:CDS
TTGGATCTAATTTAAGATTC+CGG 0.332594 8_4:+55277133 Msa1334750:three_prime_UTR
GATAAAATCAGTAGGATATA+TGG 0.337131 8_4:+55276768 Msa1334750:CDS
GAAAGTGTGGCGATTAGTTT+AGG 0.346387 8_4:+55275036 Msa1334750:CDS
TTGGGGAGAGTATGAATAAT+AGG 0.346414 8_4:+55274914 Msa1334750:CDS
GGTTATGGTGGTGGTGGAGG+TGG 0.349998 8_4:+55275066 Msa1334750:CDS
CTAATTTGAAGCCTTCGAAT+TGG 0.351114 8_4:+55275184 Msa1334750:CDS
TGCGTCGGCCGCCGGAGAAT+TGG 0.373994 8_4:+55274797 Msa1334750:CDS
TTCTGATGAGCTCTCTGTCC+TGG 0.376334 8_4:-55276732 None:intergenic
CAGCTGGTGCGGCCGCGGTT+GGG 0.377275 8_4:+55275154 Msa1334750:CDS
GCAGCTGGTGCGGCCGCGGT+TGG 0.388240 8_4:+55275153 Msa1334750:CDS
CTGTAGCACCATGAGCAAAA+TGG 0.399102 8_4:-55275266 None:intergenic
TGTAATAAGTGGGAGATGAC+TGG 0.409320 8_4:+55275219 Msa1334750:CDS
GGTGCGGCCGCGGTTGGGAA+TGG 0.416021 8_4:+55275159 Msa1334750:CDS
GGTGGTGGTGGCAGTGGAGG+AGG 0.418521 8_4:+55274655 Msa1334750:CDS
ATGACTGGGTATTGTCCCTT+TGG 0.419828 8_4:+55275234 Msa1334750:CDS
ATGAATAATAGGTCTTATAA+GGG 0.419939 8_4:+55274925 Msa1334750:CDS
GGAGGAGGAGGTGGGAGTGT+TGG 0.423488 8_4:+55275093 Msa1334750:CDS
CGGAAGATGAGTATCGTTAC+TGG 0.423843 8_4:+55274536 Msa1334750:CDS
GGTGTTGTTGTTGTTGCAGC+TGG 0.426984 8_4:+55275138 Msa1334750:CDS
TAATATAACAAGATGGGAAC+TGG 0.428850 8_4:-55276909 None:intergenic
ACTGCAGAATTACACAGATA+TGG 0.430785 8_4:+55276531 Msa1334750:intron
GTTGGTAGTGTTGGTGGTAG+TGG 0.432301 8_4:+55275111 Msa1334750:CDS
CAAGATGGTGGTGGTGGCAG+TGG 0.435000 8_4:+55274649 Msa1334750:CDS
GCGGCGGAGCCGCCGATAGC+TGG 0.440541 8_4:+55274488 Msa1334750:CDS
AAACTAGGATTTGTAATAAG+TGG 0.442008 8_4:+55275208 Msa1334750:CDS
GGTGGGAGTGTTGGTAGTGT+TGG 0.443949 8_4:+55275102 Msa1334750:CDS
GGTCAACCGACCAAGCTGAC+TGG 0.444227 8_4:+55274509 Msa1334750:CDS
TTGCTTCTCGATTTCTTACT+CGG 0.445185 8_4:-55274622 None:intergenic
AGCCTTCGAATTGGAAAACT+AGG 0.448493 8_4:+55275193 Msa1334750:CDS
GCTATCGGCGGCTCCGCCGC+CGG 0.448730 8_4:-55274485 None:intergenic
GGAGTGTGATTGAGCAGCCT+AGG 0.450759 8_4:+55274851 Msa1334750:CDS
GATGGTGGTGGTGGCAGTGG+AGG 0.455113 8_4:+55274652 Msa1334750:CDS
TACTGGAACGGAAACGATGC+TGG 0.458682 8_4:+55274553 Msa1334750:CDS
GGGAGTGTTGGTAGTGTTGG+TGG 0.465014 8_4:+55275105 Msa1334750:CDS
GTGGTGGAGGAGGAGGAGGT+GGG 0.465072 8_4:+55275085 Msa1334750:CDS
GGTGGTTATGGTGGTGGTGG+AGG 0.469378 8_4:+55275063 Msa1334750:CDS
CCGTCTTCAAATTCGCGTGC+AGG 0.475752 8_4:+55274589 Msa1334750:CDS
CCCGGTGGTTATGGTGGTGG+TGG 0.477029 8_4:+55275060 Msa1334750:CDS
GGAGGAGCTGCGTCGGCCGC+CGG 0.477423 8_4:+55274789 Msa1334750:CDS
TGTTGCGTCTGACACAAAAC+AGG 0.484755 8_4:+55276596 Msa1334750:CDS
AGTTTCAGATACCGACGGTT+GGG 0.486443 8_4:+55274878 Msa1334750:CDS
TTGTTCTGTGGATTTATGTT+CGG 0.489657 8_4:+55276995 Msa1334750:three_prime_UTR
CTTCCGTCGCCCAGTCAGCT+TGG 0.490823 8_4:-55274519 None:intergenic
TGTTGCAGCTGGTGCGGCCG+CGG 0.493113 8_4:+55275149 Msa1334750:CDS
TGAACAAAGTGCTACAATGC+TGG 0.495709 8_4:-55276960 None:intergenic
GTCAACCGACCAAGCTGACT+GGG 0.495786 8_4:+55274510 Msa1334750:CDS
AACTAGGATTTGTAATAAGT+GGG 0.496877 8_4:+55275209 Msa1334750:CDS
GTTGCATCAAATCCTCCTAC+TGG 0.500963 8_4:+55276651 Msa1334750:CDS
TGCTACAATGCTGGAAACTA+CGG 0.508443 8_4:-55276951 None:intergenic
AGTGTTGGTGGTAGTGGTAG+TGG 0.513157 8_4:+55275117 Msa1334750:CDS
ATCGGCGGCTCCGCCGCCGG+TGG 0.513840 8_4:-55274482 None:intergenic
TGTGGATTTATGTTCGGTGA+AGG 0.514081 8_4:+55277001 Msa1334750:three_prime_UTR
TATTAGATAATATAACAAGA+TGG 0.517636 8_4:-55276916 None:intergenic
CGTCGCCCAGTCAGCTTGGT+CGG 0.519038 8_4:-55274515 None:intergenic
CACGATCTGATGCACATACA+AGG 0.519654 8_4:-55277079 None:intergenic
AAAGTACAACTATCTCCATA+AGG 0.520248 8_4:-55274985 None:intergenic
CAAATCATCACCACCGGCGG+CGG 0.520822 8_4:+55274472 Msa1334750:CDS
TCAGTAGGATATATGGCGAT+TGG 0.521816 8_4:+55276775 Msa1334750:CDS
ACAATCTCCTGCCAATTCTC+CGG 0.526020 8_4:-55274808 None:intergenic
CAATGGAAGGTCTTTGTGAA+GGG 0.529629 8_4:-55276698 None:intergenic
GGAGAATTGGCAGGAGATTG+TGG 0.530968 8_4:+55274810 Msa1334750:CDS
TCCACCACCACCATAACCAC+CGG 0.534604 8_4:-55275061 None:intergenic
TATTCCCAATCTTGGAAACA+TGG 0.539439 8_4:+55276829 Msa1334750:three_prime_UTR
TCGGCCGCCGGAGAATTGGC+AGG 0.541968 8_4:+55274801 Msa1334750:CDS
GGTCCCGGTGGTTATGGTGG+TGG 0.546890 8_4:+55275057 Msa1334750:CDS
AAAATGGCATTTGTTACCAA+AGG 0.546912 8_4:-55275250 None:intergenic
GACAGAGAGCTCATCAGAAG+TGG 0.547469 8_4:+55276736 Msa1334750:CDS
ATTAGATAATATAACAAGAT+GGG 0.550868 8_4:-55276915 None:intergenic
GTGGCGATTAGTTTAGGTCC+CGG 0.552008 8_4:+55275042 Msa1334750:CDS
GGTATCTGAAACTCCTCCCT+AGG 0.552310 8_4:-55274868 None:intergenic
GGCCTCTGACTCACAATGGA+AGG 0.553370 8_4:-55276711 None:intergenic
TCGAGAAGCAATTCGCAAGA+TGG 0.554276 8_4:+55274634 Msa1334750:CDS
GAGTGTGATTGAGCAGCCTA+GGG 0.557000 8_4:+55274852 Msa1334750:CDS
AAATGGCATTTGTTACCAAA+GGG 0.557541 8_4:-55275249 None:intergenic
GTAATAAGTGGGAGATGACT+GGG 0.557999 8_4:+55275220 Msa1334750:CDS
AATTCGCAAGATGGTGGTGG+TGG 0.565492 8_4:+55274643 Msa1334750:CDS
ATCTTAAATTAGATCCAACA+TGG 0.566982 8_4:-55277128 None:intergenic
TCCATTGTGAGTCAGAGGCC+AGG 0.568730 8_4:+55276714 Msa1334750:CDS
CTATGCTGTAAGTTTCGTGT+GGG 0.569773 8_4:+55274724 Msa1334750:CDS
TGGAAAATGGCTGATGATGA+GGG 0.572671 8_4:+55276849 Msa1334750:three_prime_UTR
ATTCACAGATAATTGTTCTG+TGG 0.573853 8_4:+55276983 Msa1334750:three_prime_UTR
GACCTTCCATTGTGAGTCAG+AGG 0.580448 8_4:+55276709 Msa1334750:CDS
ATGGAAAATGGCTGATGATG+AGG 0.582450 8_4:+55276848 Msa1334750:three_prime_UTR
TATGGAGGTGGGCTTATGGA+AGG 0.583879 8_4:+55276549 Msa1334750:CDS
CGAATCGCGCGAAAGCGATA+GGG 0.588275 8_4:+55274680 Msa1334750:CDS
CCACCACCACCATAACCACC+GGG 0.590124 8_4:-55275060 None:intergenic
AGCAATTCGCAAGATGGTGG+TGG 0.591086 8_4:+55274640 Msa1334750:CDS
GAATTACACAGATATGGAGG+TGG 0.593880 8_4:+55276537 Msa1334750:intron
ACAATGGAAGGTCTTTGTGA+AGG 0.593936 8_4:-55276699 None:intergenic
TCCTGGCCTCTGACTCACAA+TGG 0.594508 8_4:-55276715 None:intergenic
TGTTGTTGTTGCAGCTGGTG+CGG 0.594830 8_4:+55275143 Msa1334750:CDS
CGACCAAGCTGACTGGGCGA+CGG 0.595148 8_4:+55274516 Msa1334750:CDS
TTGGTCGGTTGACCAGCTAT+CGG 0.595738 8_4:-55274500 None:intergenic
GATGAGTATCGTTACTGGAA+CGG 0.598603 8_4:+55274541 Msa1334750:CDS
CAAAACTCATGCTGATAATG+TGG 0.598802 8_4:+55276629 Msa1334750:CDS
GTCGTTCAAATCATCACCAC+CGG 0.600321 8_4:+55274466 Msa1334750:CDS
AGAGCTCATCAGAAGTGGAA+AGG 0.606297 8_4:+55276741 Msa1334750:CDS
TTAGGTCCCGGTGGTTATGG+TGG 0.607828 8_4:+55275054 Msa1334750:CDS
GAGTTTCAGATACCGACGGT+TGG 0.612303 8_4:+55274877 Msa1334750:CDS
AAAGTACTCGACCCAACCGT+CGG 0.614579 8_4:-55274889 None:intergenic
ACTATGCTGTAAGTTTCGTG+TGG 0.615988 8_4:+55274723 Msa1334750:CDS
ATCGAAGAATCGTGAAAGTG+TGG 0.618402 8_4:+55275023 Msa1334750:CDS
TCTTAAATTAGATCCAACAT+GGG 0.621380 8_4:-55277127 None:intergenic
AAGGTCCCGATAAAATCAGT+AGG 0.622761 8_4:+55276760 Msa1334750:CDS
GGAGGAGTTTCAGATACCGA+CGG 0.623970 8_4:+55274873 Msa1334750:CDS
TGTGATTGAGCAGCCTAGGG+AGG 0.629299 8_4:+55274855 Msa1334750:CDS
ATAGTGTGGAGGAGCTGCGT+CGG 0.631733 8_4:+55274782 Msa1334750:CDS
TAAACATCTGAACCAGTAGG+AGG 0.634383 8_4:-55276663 None:intergenic
AATTACACAGATATGGAGGT+GGG 0.635355 8_4:+55276538 Msa1334750:CDS
AGAAGCAATTCGCAAGATGG+TGG 0.638549 8_4:+55274637 Msa1334750:CDS
TGATAAACATCTGAACCAGT+AGG 0.638955 8_4:-55276666 None:intergenic
TGAATAATAGGTCTTATAAG+GGG 0.644881 8_4:+55274926 Msa1334750:CDS
CCTGCACGCGAATTTGAAGA+CGG 0.647659 8_4:-55274589 None:intergenic
TGGCTGCTCATGAAGAAGAG+AGG 0.650335 8_4:+55274830 Msa1334750:CDS
ACAATGGATTACAACAGAGA+CGG 0.651622 8_4:+55274439 None:intergenic
ATGGAAGGAGAAAGCAGAGA+CGG 0.651866 8_4:+55276564 Msa1334750:CDS
ATCTCCTGCCAATTCTCCGG+CGG 0.654680 8_4:-55274805 None:intergenic
GTTCAAATCATCACCACCGG+CGG 0.671677 8_4:+55274469 Msa1334750:CDS
GTCGGTTGACCAGCTATCGG+CGG 0.673668 8_4:-55274497 None:intergenic
GCAGAATTACACAGATATGG+AGG 0.676863 8_4:+55276534 Msa1334750:intron
CTTAAATTAGATCCAACATG+GGG 0.685023 8_4:-55277126 None:intergenic
TGGAACGGAAACGATGCTGG+TGG 0.699268 8_4:+55274556 Msa1334750:CDS
ATTAGGTCCATTCCCAACCG+CGG 0.701157 8_4:-55275166 None:intergenic
CTTCTCGATTTCTTACTCGG+CGG 0.702870 8_4:-55274619 None:intergenic
GCGATTAGTTTAGGTCCCGG+TGG 0.706931 8_4:+55275045 Msa1334750:CDS
TATGCTGTAAGTTTCGTGTG+GGG 0.708514 8_4:+55274725 Msa1334750:CDS
GTTGCGTCTGACACAAAACA+GGG 0.785414 8_4:+55276597 Msa1334750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTAAAGATTTTAATTTTA+TGG + chr8_4:55275330-55275349 Msa1334750:intron 10.0%
!!! TTTTAATTTTATGGTAATTG+AGG + chr8_4:55275339-55275358 Msa1334750:intron 15.0%
!! ATTAGATAATATAACAAGAT+GGG - chr8_4:55276918-55276937 None:intergenic 15.0%
!! TATTAGATAATATAACAAGA+TGG - chr8_4:55276919-55276938 None:intergenic 15.0%
!! AAATTACAGTTCGTTATATA+AGG - chr8_4:55274757-55274776 None:intergenic 20.0%
!!! TATGAATAATAGGTCTTATA+AGG + chr8_4:55274924-55274943 Msa1334750:CDS 20.0%
!!! ATGAATAATAGGTCTTATAA+GGG + chr8_4:55274925-55274944 Msa1334750:CDS 20.0%
!!! AAGAAATTTTATACTGAAGA+AGG + chr8_4:55274958-55274977 Msa1334750:CDS 20.0%
!! ACATAACAAAATCATCAAAA+TGG - chr8_4:55275304-55275323 None:intergenic 20.0%
!!! GGTTTTTTCTTCATTTAATA+TGG + chr8_4:55275512-55275531 Msa1334750:intron 20.0%
!!! ATATATCCTACTGATTTTAT+CGG - chr8_4:55276769-55276788 None:intergenic 20.0%
!! TGAATAATAGGTCTTATAAG+GGG + chr8_4:55274926-55274945 Msa1334750:CDS 25.0%
! AAACTAGGATTTGTAATAAG+TGG + chr8_4:55275208-55275227 Msa1334750:CDS 25.0%
! AACTAGGATTTGTAATAAGT+GGG + chr8_4:55275209-55275228 Msa1334750:CDS 25.0%
!!! GATGATTTTGTTATGTGAAT+TGG + chr8_4:55275308-55275327 Msa1334750:intron 25.0%
!!! ATAGAAAGTGCTATTAGAAT+TGG + chr8_4:55275385-55275404 Msa1334750:intron 25.0%
!! GTTATAAATAGTTTGTGTTG+TGG + chr8_4:55275411-55275430 Msa1334750:intron 25.0%
!! TTACCATTTGGATACAATTT+TGG + chr8_4:55275452-55275471 Msa1334750:intron 25.0%
!! TACCATTTGGATACAATTTT+GGG + chr8_4:55275453-55275472 Msa1334750:intron 25.0%
!! CTTTTTAATCCATACAAGAT+TGG + chr8_4:55275491-55275510 Msa1334750:intron 25.0%
! AAGAAAAAACCAATCTTGTA+TGG - chr8_4:55275503-55275522 None:intergenic 25.0%
!! TTTTGATATGTATGTGAATG+TGG + chr8_4:55275635-55275654 Msa1334750:intron 25.0%
!! TTTTCATTGTGCAAAACATA+AGG - chr8_4:55275780-55275799 None:intergenic 25.0%
! ATGAAAAGTAATCGCAAAAA+AGG - chr8_4:55275888-55275907 None:intergenic 25.0%
!! ATTTGAATTTGAAGCATACA+AGG - chr8_4:55275914-55275933 None:intergenic 25.0%
!! TTTTCTTAATCAGAAAGTGA+AGG - chr8_4:55275944-55275963 None:intergenic 25.0%
! AAAATGTATATGTGCATGTT+TGG - chr8_4:55276395-55276414 None:intergenic 25.0%
!!! TATATCCTACTGATTTTATC+GGG - chr8_4:55276768-55276787 None:intergenic 25.0%
! GATAAAATCAGTAGGATATA+TGG + chr8_4:55276768-55276787 Msa1334750:CDS 25.0%
! CAATATTATATTCCCAATCT+TGG + chr8_4:55276821-55276840 Msa1334750:three_prime_UTR 25.0%
! TCTTAAATTAGATCCAACAT+GGG - chr8_4:55277130-55277149 None:intergenic 25.0%
! ATCTTAAATTAGATCCAACA+TGG - chr8_4:55277131-55277150 None:intergenic 25.0%
!! TTGGATCTAATTTAAGATTC+CGG + chr8_4:55277133-55277152 Msa1334750:three_prime_UTR 25.0%
AAAGTACAACTATCTCCATA+AGG - chr8_4:55274988-55275007 None:intergenic 30.0%
! AAATGGCATTTGTTACCAAA+GGG - chr8_4:55275252-55275271 None:intergenic 30.0%
! AAAATGGCATTTGTTACCAA+AGG - chr8_4:55275253-55275272 None:intergenic 30.0%
TTCCCAAAATTGTATCCAAA+TGG - chr8_4:55275458-55275477 None:intergenic 30.0%
CCAAAACAAAATTGACACTA+GGG - chr8_4:55275591-55275610 None:intergenic 30.0%
ACCAAAACAAAATTGACACT+AGG - chr8_4:55275592-55275611 None:intergenic 30.0%
!!! TTTTGTTTTGGTTGATGCTA+AGG + chr8_4:55275600-55275619 Msa1334750:intron 30.0%
ATTGCATAGATTACTCAAGT+CGG - chr8_4:55275696-55275715 None:intergenic 30.0%
!!! GCAAATTTCCAAGTGTTTTT+TGG + chr8_4:55275850-55275869 Msa1334750:intron 30.0%
ACTGTAAACCAAAAAACACT+TGG - chr8_4:55275861-55275880 None:intergenic 30.0%
GAAAAGAATAGTTTCATGAG+TGG + chr8_4:55275984-55276003 Msa1334750:intron 30.0%
ACTTTCATTGCTTTGATGTT+TGG + chr8_4:55276223-55276242 Msa1334750:intron 30.0%
AAACATGCACATAATGAACA+AGG - chr8_4:55276249-55276268 None:intergenic 30.0%
TTGTTCATTATGTGCATGTT+TGG + chr8_4:55276248-55276267 Msa1334750:intron 30.0%
! TTGATACACGAGTGTTTTAT+TGG + chr8_4:55276433-55276452 Msa1334750:intron 30.0%
GGCTTAAATTGATTATCTGT+CGG + chr8_4:55276454-55276473 Msa1334750:intron 30.0%
! TTTTCCATGTTTCCAAGATT+GGG - chr8_4:55276836-55276855 None:intergenic 30.0%
! ATTTTCCATGTTTCCAAGAT+TGG - chr8_4:55276837-55276856 None:intergenic 30.0%
AATCTTGGAAACATGGAAAA+TGG + chr8_4:55276836-55276855 Msa1334750:three_prime_UTR 30.0%
TAATATAACAAGATGGGAAC+TGG - chr8_4:55276912-55276931 None:intergenic 30.0%
ATTCACAGATAATTGTTCTG+TGG + chr8_4:55276983-55277002 Msa1334750:three_prime_UTR 30.0%
TTGTTCTGTGGATTTATGTT+CGG + chr8_4:55276995-55277014 Msa1334750:three_prime_UTR 30.0%
CTTAAATTAGATCCAACATG+GGG - chr8_4:55277129-55277148 None:intergenic 30.0%
TTGCTTCTCGATTTCTTACT+CGG - chr8_4:55274625-55274644 None:intergenic 35.0%
! CTGTAATTTTGCTCATAGTG+TGG + chr8_4:55274768-55274787 Msa1334750:CDS 35.0%
! TAATTTTGCTCATAGTGTGG+AGG + chr8_4:55274771-55274790 Msa1334750:CDS 35.0%
! TTGGGGAGAGTATGAATAAT+AGG + chr8_4:55274914-55274933 Msa1334750:CDS 35.0%
CAATTCGAAGGCTTCAAATT+AGG - chr8_4:55275186-55275205 None:intergenic 35.0%
! CTAATTTGAAGCCTTCGAAT+TGG + chr8_4:55275184-55275203 Msa1334750:CDS 35.0%
!! ATCCTAGTTTTCCAATTCGA+AGG - chr8_4:55275198-55275217 None:intergenic 35.0%
!!! AACAAATGCCATTTTGCTCA+TGG + chr8_4:55275258-55275277 Msa1334750:CDS 35.0%
!! CCCTAGTGTCAATTTTGTTT+TGG + chr8_4:55275588-55275607 Msa1334750:intron 35.0%
CATTGTGCAAAACATAAGGA+AGG - chr8_4:55275776-55275795 None:intergenic 35.0%
AAGAATAGTTTCATGAGTGG+TGG + chr8_4:55275987-55276006 Msa1334750:intron 35.0%
AGAATAGTTTCATGAGTGGT+GGG + chr8_4:55275988-55276007 Msa1334750:intron 35.0%
TGTACTTAATCGCATATTCG+TGG - chr8_4:55276112-55276131 None:intergenic 35.0%
TGTTGTCAAATAGCGTCTAT+AGG + chr8_4:55276135-55276154 Msa1334750:intron 35.0%
TATGTGCATGTTTGGATTGA+CGG + chr8_4:55276256-55276275 Msa1334750:intron 35.0%
ACTGCAGAATTACACAGATA+TGG + chr8_4:55276531-55276550 Msa1334750:intron 35.0%
AATTACACAGATATGGAGGT+GGG + chr8_4:55276538-55276557 Msa1334750:CDS 35.0%
!!! TTATCAGCATGAGTTTTGGA+AGG - chr8_4:55276627-55276646 None:intergenic 35.0%
! CACATTATCAGCATGAGTTT+TGG - chr8_4:55276631-55276650 None:intergenic 35.0%
! CAAAACTCATGCTGATAATG+TGG + chr8_4:55276629-55276648 Msa1334750:CDS 35.0%
TGATAAACATCTGAACCAGT+AGG - chr8_4:55276669-55276688 None:intergenic 35.0%
GGTTCAGATGTTTATCATCT+TGG + chr8_4:55276672-55276691 Msa1334750:CDS 35.0%
TATTCCCAATCTTGGAAACA+TGG + chr8_4:55276829-55276848 Msa1334750:three_prime_UTR 35.0%
!!! CTGAGTTTTGAATGTAACAG+CGG + chr8_4:55277053-55277072 Msa1334750:three_prime_UTR 35.0%
! GATGAGTATCGTTACTGGAA+CGG + chr8_4:55274541-55274560 Msa1334750:CDS 40.0%
ACTATGCTGTAAGTTTCGTG+TGG + chr8_4:55274723-55274742 Msa1334750:CDS 40.0%
CTATGCTGTAAGTTTCGTGT+GGG + chr8_4:55274724-55274743 Msa1334750:CDS 40.0%
TATGCTGTAAGTTTCGTGTG+GGG + chr8_4:55274725-55274744 Msa1334750:CDS 40.0%
! TTGGGTCGAGTACTTTTGTT+GGG + chr8_4:55274896-55274915 Msa1334750:CDS 40.0%
ACTGAAGAAGGTTGTCCTTA+TGG + chr8_4:55274970-55274989 Msa1334750:CDS 40.0%
ATCGAAGAATCGTGAAAGTG+TGG + chr8_4:55275023-55275042 Msa1334750:CDS 40.0%
!! GAAAGTGTGGCGATTAGTTT+AGG + chr8_4:55275036-55275055 Msa1334750:CDS 40.0%
AGCCTTCGAATTGGAAAACT+AGG + chr8_4:55275193-55275212 Msa1334750:CDS 40.0%
TGTAATAAGTGGGAGATGAC+TGG + chr8_4:55275219-55275238 Msa1334750:CDS 40.0%
GTAATAAGTGGGAGATGACT+GGG + chr8_4:55275220-55275239 Msa1334750:CDS 40.0%
! TAGTTTGTGTTGTGGAGTTG+TGG + chr8_4:55275419-55275438 Msa1334750:intron 40.0%
! GGCAGTGTTTTCTTACCATT+TGG + chr8_4:55275440-55275459 Msa1334750:intron 40.0%
! CTCTTTTTGTGATCCGCAAT+CGG + chr8_4:55276194-55276213 Msa1334750:intron 40.0%
! TGAAAGTATGTTGCCGATTG+CGG - chr8_4:55276210-55276229 None:intergenic 40.0%
AGACTGGCATGACATAATCA+TGG - chr8_4:55276362-55276381 None:intergenic 40.0%
TATGTGCATGTTTGGATCGA+CGG - chr8_4:55276387-55276406 None:intergenic 40.0%
GCAGAATTACACAGATATGG+AGG + chr8_4:55276534-55276553 Msa1334750:intron 40.0%
GAATTACACAGATATGGAGG+TGG + chr8_4:55276537-55276556 Msa1334750:intron 40.0%
TAAACATCTGAACCAGTAGG+AGG - chr8_4:55276666-55276685 None:intergenic 40.0%
CAATGGAAGGTCTTTGTGAA+GGG - chr8_4:55276701-55276720 None:intergenic 40.0%
ACAATGGAAGGTCTTTGTGA+AGG - chr8_4:55276702-55276721 None:intergenic 40.0%
AAGGTCCCGATAAAATCAGT+AGG + chr8_4:55276760-55276779 Msa1334750:CDS 40.0%
TCAGTAGGATATATGGCGAT+TGG + chr8_4:55276775-55276794 Msa1334750:CDS 40.0%
! ATGGAAAATGGCTGATGATG+AGG + chr8_4:55276848-55276867 Msa1334750:three_prime_UTR 40.0%
! TGGAAAATGGCTGATGATGA+GGG + chr8_4:55276849-55276868 Msa1334750:three_prime_UTR 40.0%
! TCATGACGTTTTCAGCTACA+TGG - chr8_4:55276886-55276905 None:intergenic 40.0%
TGCTACAATGCTGGAAACTA+CGG - chr8_4:55276954-55276973 None:intergenic 40.0%
! TGAACAAAGTGCTACAATGC+TGG - chr8_4:55276963-55276982 None:intergenic 40.0%
! TGTGGATTTATGTTCGGTGA+AGG + chr8_4:55277001-55277020 Msa1334750:three_prime_UTR 40.0%
! GTTCTTTTGTTACCCCATGT+TGG + chr8_4:55277114-55277133 Msa1334750:three_prime_UTR 40.0%
GTCGTTCAAATCATCACCAC+CGG + chr8_4:55274466-55274485 Msa1334750:CDS 45.0%
! CGGAAGATGAGTATCGTTAC+TGG + chr8_4:55274536-55274555 Msa1334750:CDS 45.0%
CTTCTCGATTTCTTACTCGG+CGG - chr8_4:55274622-55274641 None:intergenic 45.0%
TCGAGAAGCAATTCGCAAGA+TGG + chr8_4:55274634-55274653 Msa1334750:CDS 45.0%
AGAAGCAATTCGCAAGATGG+TGG + chr8_4:55274637-55274656 Msa1334750:CDS 45.0%
ACAATCTCCTGCCAATTCTC+CGG - chr8_4:55274811-55274830 None:intergenic 45.0%
AGTTTCAGATACCGACGGTT+GGG + chr8_4:55274878-55274897 Msa1334750:CDS 45.0%
! GTTGGGTCGAGTACTTTTGT+TGG + chr8_4:55274895-55274914 Msa1334750:CDS 45.0%
! TGGGTCGAGTACTTTTGTTG+GGG + chr8_4:55274897-55274916 Msa1334750:CDS 45.0%
! ATGACTGGGTATTGTCCCTT+TGG + chr8_4:55275234-55275253 Msa1334750:CDS 45.0%
CTGTAGCACCATGAGCAAAA+TGG - chr8_4:55275269-55275288 None:intergenic 45.0%
!!! CATTTTGCTCATGGTGCTAC+AGG + chr8_4:55275267-55275286 Msa1334750:CDS 45.0%
TTGACACTAGGGACAATGCA+GGG - chr8_4:55275580-55275599 None:intergenic 45.0%
ATTGACACTAGGGACAATGC+AGG - chr8_4:55275581-55275600 None:intergenic 45.0%
! GGTGGGTTCAGAATGAAAGA+TGG + chr8_4:55276005-55276024 Msa1334750:intron 45.0%
TAGGATAGCACAGTACGTAG+TGG + chr8_4:55276154-55276173 Msa1334750:intron 45.0%
ATGGAAGGAGAAAGCAGAGA+CGG + chr8_4:55276564-55276583 Msa1334750:CDS 45.0%
TGTTGCGTCTGACACAAAAC+AGG + chr8_4:55276596-55276615 Msa1334750:CDS 45.0%
GTTGCGTCTGACACAAAACA+GGG + chr8_4:55276597-55276616 Msa1334750:CDS 45.0%
GTTGCATCAAATCCTCCTAC+TGG + chr8_4:55276651-55276670 Msa1334750:CDS 45.0%
AGAGCTCATCAGAAGTGGAA+AGG + chr8_4:55276741-55276760 Msa1334750:CDS 45.0%
CACGATCTGATGCACATACA+AGG - chr8_4:55277082-55277101 None:intergenic 45.0%
GTTCAAATCATCACCACCGG+CGG + chr8_4:55274469-55274488 Msa1334750:CDS 50.0%
TTGGTCGGTTGACCAGCTAT+CGG - chr8_4:55274503-55274522 None:intergenic 50.0%
TACTGGAACGGAAACGATGC+TGG + chr8_4:55274553-55274572 Msa1334750:CDS 50.0%
CCTGCACGCGAATTTGAAGA+CGG - chr8_4:55274592-55274611 None:intergenic 50.0%
! AGCAATTCGCAAGATGGTGG+TGG + chr8_4:55274640-55274659 Msa1334750:CDS 50.0%
!! AATTCGCAAGATGGTGGTGG+TGG + chr8_4:55274643-55274662 Msa1334750:CDS 50.0%
GGAGAATTGGCAGGAGATTG+TGG + chr8_4:55274810-55274829 Msa1334750:CDS 50.0%
TGGCTGCTCATGAAGAAGAG+AGG + chr8_4:55274830-55274849 Msa1334750:CDS 50.0%
GAGTGTGATTGAGCAGCCTA+GGG + chr8_4:55274852-55274871 Msa1334750:CDS 50.0%
! GGTATCTGAAACTCCTCCCT+AGG - chr8_4:55274871-55274890 None:intergenic 50.0%
GGAGGAGTTTCAGATACCGA+CGG + chr8_4:55274873-55274892 Msa1334750:CDS 50.0%
GAGTTTCAGATACCGACGGT+TGG + chr8_4:55274877-55274896 Msa1334750:CDS 50.0%
! AAAGTACTCGACCCAACCGT+CGG - chr8_4:55274892-55274911 None:intergenic 50.0%
!! GTGGCGATTAGTTTAGGTCC+CGG + chr8_4:55275042-55275061 Msa1334750:CDS 50.0%
AGTTTAGGTCCCGGTGGTTA+TGG + chr8_4:55275051-55275070 Msa1334750:CDS 50.0%
!! GTTGGTAGTGTTGGTGGTAG+TGG + chr8_4:55275111-55275130 Msa1334750:CDS 50.0%
!! AGTGTTGGTGGTAGTGGTAG+TGG + chr8_4:55275117-55275136 Msa1334750:CDS 50.0%
!! GGTGTTGTTGTTGTTGCAGC+TGG + chr8_4:55275138-55275157 Msa1334750:CDS 50.0%
! TGTTGTTGTTGCAGCTGGTG+CGG + chr8_4:55275143-55275162 Msa1334750:CDS 50.0%
ATTAGGTCCATTCCCAACCG+CGG - chr8_4:55275169-55275188 None:intergenic 50.0%
! CAGATATGGAGGTGGGCTTA+TGG + chr8_4:55276545-55276564 Msa1334750:CDS 50.0%
! TATGGAGGTGGGCTTATGGA+AGG + chr8_4:55276549-55276568 Msa1334750:CDS 50.0%
GACCTTCCATTGTGAGTCAG+AGG + chr8_4:55276709-55276728 Msa1334750:CDS 50.0%
TTCTGATGAGCTCTCTGTCC+TGG - chr8_4:55276735-55276754 None:intergenic 50.0%
GACAGAGAGCTCATCAGAAG+TGG + chr8_4:55276736-55276755 Msa1334750:CDS 50.0%
GTCAACCGACCAAGCTGACT+GGG + chr8_4:55274510-55274529 Msa1334750:CDS 55.0%
TGGAACGGAAACGATGCTGG+TGG + chr8_4:55274556-55274575 Msa1334750:CDS 55.0%
CCGTCTTCAAATTCGCGTGC+AGG + chr8_4:55274589-55274608 Msa1334750:CDS 55.0%
TCGAATCGCGCGAAAGCGAT+AGG + chr8_4:55274679-55274698 Msa1334750:CDS 55.0%
CGAATCGCGCGAAAGCGATA+GGG + chr8_4:55274680-55274699 Msa1334750:CDS 55.0%
ATAGTGTGGAGGAGCTGCGT+CGG + chr8_4:55274782-55274801 Msa1334750:CDS 55.0%
ATCTCCTGCCAATTCTCCGG+CGG - chr8_4:55274808-55274827 None:intergenic 55.0%
GGAGTGTGATTGAGCAGCCT+AGG + chr8_4:55274851-55274870 Msa1334750:CDS 55.0%
TGTGATTGAGCAGCCTAGGG+AGG + chr8_4:55274855-55274874 Msa1334750:CDS 55.0%
!! GCGATTAGTTTAGGTCCCGG+TGG + chr8_4:55275045-55275064 Msa1334750:CDS 55.0%
TTAGGTCCCGGTGGTTATGG+TGG + chr8_4:55275054-55275073 Msa1334750:CDS 55.0%
TCCACCACCACCATAACCAC+CGG - chr8_4:55275064-55275083 None:intergenic 55.0%
!! GGTGGGAGTGTTGGTAGTGT+TGG + chr8_4:55275102-55275121 Msa1334750:CDS 55.0%
!! GGGAGTGTTGGTAGTGTTGG+TGG + chr8_4:55275105-55275124 Msa1334750:CDS 55.0%
AGTGCTACAGCGCTATCGTG+TGG + chr8_4:55276060-55276079 Msa1334750:intron 55.0%
CGGCGAGTTTAGCGAAATCG+CGG + chr8_4:55276276-55276295 Msa1334750:intron 55.0%
CGAGTTTAGCGAAATCGCGG+TGG + chr8_4:55276279-55276298 Msa1334750:intron 55.0%
GTTTGGATCGACGGTGAGAC+TGG - chr8_4:55276378-55276397 None:intergenic 55.0%
GGCCTCTGACTCACAATGGA+AGG - chr8_4:55276714-55276733 None:intergenic 55.0%
TCCATTGTGAGTCAGAGGCC+AGG + chr8_4:55276714-55276733 Msa1334750:CDS 55.0%
TCCTGGCCTCTGACTCACAA+TGG - chr8_4:55276718-55276737 None:intergenic 55.0%
CAAATCATCACCACCGGCGG+CGG + chr8_4:55274472-55274491 Msa1334750:CDS 60.0%
GTCGGTTGACCAGCTATCGG+CGG - chr8_4:55274500-55274519 None:intergenic 60.0%
GGTCAACCGACCAAGCTGAC+TGG + chr8_4:55274509-55274528 Msa1334750:CDS 60.0%
!! CAAGATGGTGGTGGTGGCAG+TGG + chr8_4:55274649-55274668 Msa1334750:CDS 60.0%
CCACCACCACCATAACCACC+GGG - chr8_4:55275063-55275082 None:intergenic 60.0%
!! GGTGGTTATGGTGGTGGTGG+AGG + chr8_4:55275063-55275082 Msa1334750:CDS 60.0%
!! GGTTATGGTGGTGGTGGAGG+TGG + chr8_4:55275066-55275085 Msa1334750:CDS 60.0%
!! TATGGTGGTGGTGGAGGTGG+TGG + chr8_4:55275069-55275088 Msa1334750:CDS 60.0%
CGTCGCCCAGTCAGCTTGGT+CGG - chr8_4:55274518-55274537 None:intergenic 65.0%
CGACCAAGCTGACTGGGCGA+CGG + chr8_4:55274516-55274535 Msa1334750:CDS 65.0%
CTTCCGTCGCCCAGTCAGCT+TGG - chr8_4:55274522-55274541 None:intergenic 65.0%
!! GATGGTGGTGGTGGCAGTGG+AGG + chr8_4:55274652-55274671 Msa1334750:CDS 65.0%
GGTCCCGGTGGTTATGGTGG+TGG + chr8_4:55275057-55275076 Msa1334750:CDS 65.0%
!! CCCGGTGGTTATGGTGGTGG+TGG + chr8_4:55275060-55275079 Msa1334750:CDS 65.0%
! GTGGTGGAGGAGGAGGAGGT+GGG + chr8_4:55275085-55275104 Msa1334750:CDS 65.0%
! GGAGGAGGAGGTGGGAGTGT+TGG + chr8_4:55275093-55275112 Msa1334750:CDS 65.0%
!! GGTGGTGGTGGCAGTGGAGG+AGG + chr8_4:55274655-55274674 Msa1334750:CDS 70.0%
! TGCGTCGGCCGCCGGAGAAT+TGG + chr8_4:55274797-55274816 Msa1334750:CDS 70.0%
TCGGCCGCCGGAGAATTGGC+AGG + chr8_4:55274801-55274820 Msa1334750:CDS 70.0%
!! GGTGGTGGTGGAGGTGGTGG+AGG + chr8_4:55275072-55275091 Msa1334750:CDS 70.0%
!! GGTGGTGGAGGTGGTGGAGG+AGG + chr8_4:55275075-55275094 Msa1334750:CDS 70.0%
!! GGTGGAGGTGGTGGAGGAGG+AGG + chr8_4:55275078-55275097 Msa1334750:CDS 70.0%
!! GGAGGTGGTGGAGGAGGAGG+AGG + chr8_4:55275081-55275100 Msa1334750:CDS 70.0%
!! GGTGGTGGAGGAGGAGGAGG+TGG + chr8_4:55275084-55275103 Msa1334750:CDS 70.0%
TGTTGCAGCTGGTGCGGCCG+CGG + chr8_4:55275149-55275168 Msa1334750:CDS 70.0%
CAGCTGGTGCGGCCGCGGTT+GGG + chr8_4:55275154-55275173 Msa1334750:CDS 75.0%
GGTGCGGCCGCGGTTGGGAA+TGG + chr8_4:55275159-55275178 Msa1334750:CDS 75.0%
! GCTATCGGCGGCTCCGCCGC+CGG - chr8_4:55274488-55274507 None:intergenic 80.0%
! GCGGCGGAGCCGCCGATAGC+TGG + chr8_4:55274488-55274507 Msa1334750:CDS 80.0%
!! GGAGGAGCTGCGTCGGCCGC+CGG + chr8_4:55274789-55274808 Msa1334750:CDS 80.0%
! GCAGCTGGTGCGGCCGCGGT+TGG + chr8_4:55275153-55275172 Msa1334750:CDS 80.0%
! ATCGGCGGCTCCGCCGCCGG+TGG - chr8_4:55274485-55274504 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 55274442 55277166 55274442 ID=Msa1334750;Name=Msa1334750
chr8_4 mRNA 55274442 55277166 55274442 ID=Msa1334750-mRNA-1;Parent=Msa1334750;Name=Msa1334750-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|1|1|2|351|374
chr8_4 exon 55274442 55275288 55274442 ID=Msa1334750-mRNA-1:exon:17241;Parent=Msa1334750-mRNA-1
chr8_4 exon 55276538 55277166 55276538 ID=Msa1334750-mRNA-1:exon:17242;Parent=Msa1334750-mRNA-1
chr8_4 CDS 55274442 55275288 55274442 ID=Msa1334750-mRNA-1:cds;Parent=Msa1334750-mRNA-1
chr8_4 CDS 55276538 55276815 55276538 ID=Msa1334750-mRNA-1:cds;Parent=Msa1334750-mRNA-1
chr8_4 three_prime_UTR 55276816 55277166 55276816 ID=Msa1334750-mRNA-1:three_prime_utr;Parent=Msa1334750-mRNA-1
Gene Sequence

>Msa1334750

ATGGATTACAACAGAGACGGCAACGTCGTTCAAATCATCACCACCGGCGGCGGAGCCGCCGATAGCTGGTCAACCGACCAAGCTGACTGGGCGACGGAAGATGAGTATCGTTACTGGAACGGAAACGATGCTGGTGGCGAAACAACTCCGTCTTCAAATTCGCGTGCAGGAAGTGAGCCGCCGAGTAAGAAATCGAGAAGCAATTCGCAAGATGGTGGTGGTGGCAGTGGAGGAGGATCGAATCGCGCGAAAGCGATAGGGAAAATGTTTTTTAAGACGAAACTATGCTGTAAGTTTCGTGTGGGGACATGTCCTTATATAACGAACTGTAATTTTGCTCATAGTGTGGAGGAGCTGCGTCGGCCGCCGGAGAATTGGCAGGAGATTGTGGCTGCTCATGAAGAAGAGAGGAGTGTGATTGAGCAGCCTAGGGAGGAGTTTCAGATACCGACGGTTGGGTCGAGTACTTTTGTTGGGGAGAGTATGAATAATAGGTCTTATAAGGGGAGACATTGTAAGAAATTTTATACTGAAGAAGGTTGTCCTTATGGAGATAGTTGTACTTTTCTTCATGATGAGCAATCGAAGAATCGTGAAAGTGTGGCGATTAGTTTAGGTCCCGGTGGTTATGGTGGTGGTGGAGGTGGTGGAGGAGGAGGAGGTGGGAGTGTTGGTAGTGTTGGTGGTAGTGGTAGTGGTGTTGTTGTTGTTGCAGCTGGTGCGGCCGCGGTTGGGAATGGACCTAATTTGAAGCCTTCGAATTGGAAAACTAGGATTTGTAATAAGTGGGAGATGACTGGGTATTGTCCCTTTGGTAACAAATGCCATTTTGCTCATGGTGCTACAGAATTACACAGATATGGAGGTGGGCTTATGGAAGGAGAAAGCAGAGACGGTGTTTCTGTTGTTGCGTCTGACACAAAACAGGGTGTACCTTCCAAAACTCATGCTGATAATGTGGTTGCATCAAATCCTCCTACTGGTTCAGATGTTTATCATCTTGGAGTCCCTTCACAAAGACCTTCCATTGTGAGTCAGAGGCCAGGACAGAGAGCTCATCAGAAGTGGAAAGGTCCCGATAAAATCAGTAGGATATATGGCGATTGGATCGATGACTTCGAATAG
Protein sequence

>Msa1334750

MDYNRDGNVVQIITTGGGAADSWSTDQADWATEDEYRYWNGNDAGGETTPSSNSRAGSEPPSKKSRSNSQDGGGGSGGGSNRAKAIGKMFFKTKLCCKFRVGTCPYITNCNFAHSVEELRRPPENWQEIVAAHEEERSVIEQPREEFQIPTVGSSTFVGESMNNRSYKGRHCKKFYTEEGCPYGDSCTFLHDEQSKNRESVAISLGPGGYGGGGGGGGGGGGSVGSVGGSGSGVVVVAAGAAAVGNGPNLKPSNWKTRICNKWEMTGYCPFGNKCHFAHGATELHRYGGGLMEGESRDGVSVVASDTKQGVPSKTHADNVVASNPPTGSDVYHLGVPSQRPSIVSQRPGQRAHQKWKGPDKISRIYGDWIDDFE*