AlfalfaGEDB | Msa1213640

M. sativa cultivar zhongmu-4 / Msa1213640

KEGG: K15397
GO: GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
PFAM: PF08392.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1213640	sp\|O48780\|KCS11_ARATH	83.865	502	81	0	63	564	8	509	0.0	904

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1213640	A0A396IHT1	98.800	500	6	0	65	564	1	500	0.0	1025

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0081520	Msa1213640	0.807870	4.091959e-50	-8.615850e-47
Msa0134180	Msa1213640	0.820689	6.005071e-53	-8.615850e-47
Msa0180930	Msa1213640	0.808005	3.830846e-50	-8.615850e-47
Msa0292400	Msa1213640	0.837369	5.414319e-57	-8.615850e-47
Msa0600430	Msa1213640	0.801825	7.500504e-49	-8.615850e-47
Msa0738040	Msa1213640	0.811975	5.349723e-51	-8.615850e-47
Msa1020670	Msa1213640	0.811034	8.565624e-51	-8.615850e-47
Msa1045140	Msa1213640	0.812908	3.346428e-51	-8.615850e-47
Msa1124110	Msa1213640	0.811611	6.419692e-51	-8.615850e-47
Msa1213640	Msa1232710	0.819556	1.091638e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1213640	MtrunA17_Chr4g0063011	98.800	500	6	0	65	564	1	500	1025
Msa1213640	MtrunA17_Chr1g0188051	75.351	499	123	0	66	564	14	512	808
Msa1213640	MtrunA17_Chr1g0166191	75.862	493	119	0	72	564	26	518	797
Msa1213640	MtrunA17_Chr4g0035081	67.276	492	160	1	67	558	12	502	708
Msa1213640	MtrunA17_Chr2g0306781	85.856	403	52	1	167	564	3	405	701
Msa1213640	MtrunA17_Chr7g0276251	65.139	502	160	5	70	559	31	529	671
Msa1213640	MtrunA17_Chr1g0209881	60.285	491	192	2	72	561	5	493	625
Msa1213640	MtrunA17_Chr5g0423521	58.859	491	199	2	72	561	5	493	612
Msa1213640	MtrunA17_Chr7g0250661	90.508	295	28	0	270	564	1	295	572
Msa1213640	MtrunA17_Chr3g0136561	55.315	461	195	3	109	558	443	903	562

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1213640	AT2G26640.1	83.865	502	81	0	63	564	8	509	904
Msa1213640	AT1G04220.1	71.287	505	136	5	68	564	18	521	750
Msa1213640	AT5G43760.1	72.819	493	128	3	78	564	34	526	722
Msa1213640	AT2G16280.1	67.276	492	160	1	67	558	20	510	711
Msa1213640	AT1G19440.1	68.033	488	155	1	69	556	26	512	711
Msa1213640	AT1G01120.1	63.030	495	177	3	70	558	32	526	668
Msa1213640	AT1G68530.1	59.759	497	197	2	65	561	1	494	639
Msa1213640	AT4G34510.1	62.422	479	169	4	83	558	15	485	632
Msa1213640	AT1G25450.1	59.592	490	197	1	72	561	1	489	622
Msa1213640	AT2G26250.1	54.457	516	202	5	70	556	37	548	593

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Find 146 sgRNAs with CRISPR-Local

Find 380 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTAATATCACTACGCT+AGG	0.493178	8_1:-73825431	Msa1213640:CDS
AAAACTTGCAGCTATCACCA+TGG	0.706018	8_1:-73825244	Msa1213640:CDS
AAAATACCAATTGATCACCA+CGG	0.722409	8_1:-73825576	Msa1213640:CDS
AAACAGCTCTACCTCCGGCA+TGG	0.599316	8_1:+73825282	None:intergenic
AAACTAATATCACTACGCTA+GGG	0.626894	8_1:-73825430	Msa1213640:CDS
AAAGATCTTATGGCAGTTGC+CGG	0.419635	8_1:-73825464	Msa1213640:CDS
AAAGCGTGGACAGGAAAACA+AGG	0.654849	8_1:+73826236	None:intergenic
AAATTGAAGTATGTTAAACT+TGG	0.445451	8_1:-73826415	Msa1213640:CDS
AAATTGGAAAACATGGGAAG+AGG	0.571781	8_1:+73826947	None:intergenic
AACAGTAGTTGTTCTGATGT+TGG	0.477505	8_1:+73825395	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCAGTGAGTAATGAAAAAT+TGG	+	chr8_1:73824762-73824781	None:intergenic	30.0%
CATTGTGGATTTATACACAA+AGG	-	chr8_1:73824836-73824855	Msa1213640:three_prime_UTR	30.0%
TACTCAACATGAAAATCAAG+TGG	+	chr8_1:73824941-73824960	None:intergenic	30.0%
AATCATGAGCAACAACAAAA+GGG	+	chr8_1:73825119-73825138	None:intergenic	30.0%
AAATCATGAGCAACAACAAA+AGG	+	chr8_1:73825120-73825139	None:intergenic	30.0%
TATCACTATCTAATCACTCA+TGG	-	chr8_1:73825299-73825318	Msa1213640:CDS	30.0%
CCCAAATATCATAAAAGTCT+TGG	+	chr8_1:73825388-73825407	None:intergenic	30.0%
TGTAACATGAGAAATCAACA+AGG	+	chr8_1:73825508-73825527	None:intergenic	30.0%
CTTACTAAATAGCTCATCAA+TGG	+	chr8_1:73825747-73825766	None:intergenic	30.0%
TATATCTTTAGGCTTTACTG+TGG	+	chr8_1:73825771-73825790	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	73824631	73827081	73824631	ID=Msa1213640;Name=Msa1213640
chr8_1	mRNA	73824631	73827081	73824631	ID=Msa1213640-mRNA-1;Parent=Msa1213640;Name=Msa1213640-mRNA-1;_AED=0.01;_eAED=0.01;_QI=121\|0\|0.66\|0.66\|0\|0\|3\|349\|564
chr8_1	exon	73824631	73826519	73824631	ID=Msa1213640-mRNA-1:exon:25232;Parent=Msa1213640-mRNA-1
chr8_1	exon	73826739	73826787	73826739	ID=Msa1213640-mRNA-1:exon:25231;Parent=Msa1213640-mRNA-1
chr8_1	exon	73826855	73827081	73826855	ID=Msa1213640-mRNA-1:exon:25230;Parent=Msa1213640-mRNA-1
chr8_1	five_prime_UTR	73826961	73827081	73826961	ID=Msa1213640-mRNA-1:five_prime_utr;Parent=Msa1213640-mRNA-1
chr8_1	CDS	73826855	73826960	73826855	ID=Msa1213640-mRNA-1:cds;Parent=Msa1213640-mRNA-1
chr8_1	CDS	73826739	73826787	73826739	ID=Msa1213640-mRNA-1:cds;Parent=Msa1213640-mRNA-1
chr8_1	CDS	73824980	73826519	73824980	ID=Msa1213640-mRNA-1:cds;Parent=Msa1213640-mRNA-1
chr8_1	three_prime_UTR	73824631	73824979	73824631	ID=Msa1213640-mRNA-1:three_prime_utr;Parent=Msa1213640-mRNA-1

Gene Sequence

>Msa1213640

ATGTTTTCCAATTTTTCATTACTCACTGCATGCTTCTTCTTTGTTTCACTTGTATCTATCTGCAATCTTCTTCCTGAACGAATGCATTGTGGATTTATACACAAAGTCTATTTTCAATTCCACTTGATTTTCATGTTGAGTACCATTCAATGCATGGCAAAAATGACAAATGGTAGATTAGATACACCCTTGATGCCAAATTCATCCAGGAACTTGCCTGATTTCAAGAAATCTGTTAAATTGAAGTATGTTAAACTTGGTTATCACTATCTAATCACTCATGGAATGTACCTTTTTCTTTCCCCACTTGTGGTGTTGATTGCTGCTCAGATATCCACTTTTTCCATCCAAGACTTTTATGATATTTGGGAGCATCTACAATACAATTTGGTATCTGTCGTTTTTTGTTCAACTCTCCTTGTTTTCCTGTCCACGCTTTACTTTTTGACTCGTCCGAAACCGGTGTACCTTGTTGATTTCTCATGTTACAAGCCTGAAGAATCGCGTAAGTGCACGAAAAGGAAATTTATGGATCAGTCTAGGATGATCAGTACTTTTACGGAGGAAAATCTTGAATTTCAGCGAAAGATTCTTGAGAGATCGGGACTCGGGGAATCTACTTATCTTCCAGAGGCTGTTCTTAACTTTCCTCCTAATCCTTCAATGAAAGAAGCTAGAAAAGAAGCTGAGACTGTGATGTTCGGTGCCATTGATGAGCTATTTAGTAAGACCACAGTAAAGCCTAAAGATATAGGAATTCTGATTGTAAATTGTAGCTTGTTCAACCCAACTCCGTCGCTATCTTCAATGATTGTCAATCATTACAAGCTTCGAGGGAATATAAAGAGCTACAACCTAGGTGGGATGGGTTGCAGTGCGGGGCTGATCTCGATTGATCTTGCTAAAGATCTTCTCCAGGCCAATCCTAATTCCTATGCATTGGTCATTAGCATGGAGAATATCACATTGAATTGGTATTTTGGAAACGATCGGTCGAAACTTGTTTCTAATTGTTTGTTTCGAATGGGAGGAGCTGTGGTTCTGCTTTCCAATAAAACCTCAGACAGAAGAAGATCAAAATACCAATTGATCACCACGGTTCGCACTAACAAGGCTTCTGATGACAAGTGTTTTAGCTGTGTAACCCAAGAAGAAGATGCCAATGGCAATATTGGTGTTACCTTGTCAAAAGATCTTATGGCAGTTGCCGGTGATGCTTTAAAAACTAATATCACTACGCTAGGGCCTCTCGTACTTCCAACATCAGAACAACTACTGTTCTTCACCACATTGGTTGGGAAGAAACTTTTCAAGATGAAGATAAAACCTTATATTCCTGATTTCAAGCTAGCTTTTGAACATTTTTGCATCCATGCCGGAGGTAGAGCTGTTTTGGATGAACTGGAGAAAAACTTGCAGCTATCACCATGGCATATGGAGCCATCAAGGATGACACTTTATCGTTTTGGAAACACATCTAGCAGTTCACTTTGGTATGAATTGGCTTACACAGAAGCCAAAGGGAGAATTAAGAAGGGAGATAGAACATGGCAAATAGCATTTGGTTCTGGATTCAAGTGCAACAGTGCAGTGTGGAAGGCTCTCAGGACAATCAACCCTGTGAAGGAGAAAAGCCCTTGGATAGATGAGATTGACCAGTTTCCTGTTGATGTTCCAAAGGTATCTACCATCTGA

Protein sequence

>Msa1213640

MFSNFSLLTACFFFVSLVSICNLLPERMHCGFIHKVYFQFHLIFMLSTIQCMAKMTNGRLDTPLMPNSSRNLPDFKKSVKLKYVKLGYHYLITHGMYLFLSPLVVLIAAQISTFSIQDFYDIWEHLQYNLVSVVFCSTLLVFLSTLYFLTRPKPVYLVDFSCYKPEESRKCTKRKFMDQSRMISTFTEENLEFQRKILERSGLGESTYLPEAVLNFPPNPSMKEARKEAETVMFGAIDELFSKTTVKPKDIGILIVNCSLFNPTPSLSSMIVNHYKLRGNIKSYNLGGMGCSAGLISIDLAKDLLQANPNSYALVISMENITLNWYFGNDRSKLVSNCLFRMGGAVVLLSNKTSDRRRSKYQLITTVRTNKASDDKCFSCVTQEEDANGNIGVTLSKDLMAVAGDALKTNITTLGPLVLPTSEQLLFFTTLVGKKLFKMKIKPYIPDFKLAFEHFCIHAGGRAVLDELEKNLQLSPWHMEPSRMTLYRFGNTSSSSLWYELAYTEAKGRIKKGDRTWQIAFGSGFKCNSAVWKALRTINPVKEKSPWIDEIDQFPVDVPKVSTI*