AlfalfaGEDB | Msa1331230

M. sativa cultivar zhongmu-4 / Msa1331230

KEGG: K15397
GO: GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
PFAM: PF08392.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331230	sp\|O48780\|KCS11_ARATH	83.865	502	81	0	86	587	8	509	0.0	899

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331230	A0A396IHT1	98.800	500	6	0	88	587	1	500	0.0	1021

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0081520	Msa1331230	0.808176	3.522656e-50	-8.615850e-47
Msa0664060	Msa1331230	0.802298	5.996119e-49	-8.615850e-47
Msa0134180	Msa1331230	0.814168	1.767468e-51	-8.615850e-47
Msa0136650	Msa1331230	0.815697	8.093511e-52	-8.615850e-47
Msa0180930	Msa1331230	0.807568	4.744348e-50	-8.615850e-47
Msa1229500	Msa1331230	0.803438	3.485308e-49	-8.615850e-47
Msa1232710	Msa1331230	0.830569	2.728351e-55	-8.615850e-47
Msa1261200	Msa1331230	0.881833	1.728137e-70	-8.615850e-47
Msa1261550	Msa1331230	0.981357	2.154471e-152	-8.615850e-47
Msa1271990	Msa1331230	0.801234	9.913161e-49	-8.615850e-47
Msa1304310	Msa1331230	0.853824	1.850693e-61	-8.615850e-47
Msa1304760	Msa1331230	0.971838	8.499653e-134	-8.615850e-47
Msa1313300	Msa1331230	0.825968	3.508712e-54	-8.615850e-47
Msa0412150	Msa1331230	0.802819	4.680725e-49	-8.615850e-47
Msa0430260	Msa1331230	0.801383	9.238990e-49	-8.615850e-47
Msa0981320	Msa1331230	0.804522	2.074376e-49	-8.615850e-47
Msa1018690	Msa1331230	0.805103	1.569165e-49	-8.615850e-47
Msa1020670	Msa1331230	0.808858	2.518740e-50	-8.615850e-47
Msa1045140	Msa1331230	0.819622	1.054575e-52	-8.615850e-47
Msa0292400	Msa1331230	0.842635	2.293129e-58	-8.615850e-47
Msa0298500	Msa1331230	0.805246	1.464490e-49	-8.615850e-47
Msa1113340	Msa1331230	0.819219	1.303030e-52	-8.615850e-47
Msa1213640	Msa1331230	0.978309	1.477020e-145	-8.615850e-47
Msa0938120	Msa1331230	0.810050	1.397370e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331230	MtrunA17_Chr4g0063011	98.800	500	6	0	88	587	1	500	0.0	1021
Msa1331230	MtrunA17_Chr1g0188051	75.351	499	123	0	89	587	14	512	0.0	806
Msa1331230	MtrunA17_Chr1g0166191	75.862	493	119	0	95	587	26	518	0.0	793
Msa1331230	MtrunA17_Chr4g0035081	67.276	492	160	1	90	581	12	502	0.0	704
Msa1331230	MtrunA17_Chr2g0306781	85.856	403	52	1	190	587	3	405	0.0	699
Msa1331230	MtrunA17_Chr7g0276251	65.139	502	160	5	93	582	31	529	0.0	667
Msa1331230	MtrunA17_Chr1g0209881	60.285	491	192	2	95	584	5	493	0.0	624
Msa1331230	MtrunA17_Chr5g0423521	58.859	491	199	2	95	584	5	493	0.0	611
Msa1331230	MtrunA17_Chr7g0250661	90.508	295	28	0	293	587	1	295	0.0	567
Msa1331230	MtrunA17_Chr3g0136561	55.315	461	195	3	132	581	443	903	0.0	559
Msa1331230	MtrunA17_Chr6g0480561	60.739	433	169	1	152	583	38	470	0.0	547
Msa1331230	MtrunA17_Chr2g0282281	53.333	480	214	4	95	573	33	503	1.74e-170	493
Msa1331230	MtrunA17_Chr2g0277841	51.707	410	195	3	174	582	45	452	7.93e-152	444
Msa1331230	MtrunA17_Chr2g0295781	49.180	427	212	4	158	581	27	451	2.96e-148	435
Msa1331230	MtrunA17_Chr7g0276241	65.398	289	93	2	293	579	1	284	1.26e-137	402
Msa1331230	MtrunA17_Chr1g0204301	46.078	408	219	1	173	579	77	484	1.17e-130	391
Msa1331230	MtrunA17_Chr3g0115021	45.012	411	224	2	170	579	74	483	3.58e-124	374
Msa1331230	MtrunA17_Chr3g0115041	45.012	411	224	2	170	579	74	483	4.30e-124	374
Msa1331230	MtrunA17_Chr6g0469631	45.098	408	222	2	173	579	76	482	2.20e-116	354
Msa1331230	MtrunA17_Chr6g0461631	61.569	255	98	0	328	582	3	257	2.77e-114	341
Msa1331230	MtrunA17_Chr4g0030601	40.046	437	244	5	152	578	5	433	2.29e-107	330
Msa1331230	MtrunA17_Chr7g0250651	83.333	186	30	1	78	262	1	186	2.63e-106	318
Msa1331230	MtrunA17_Chr4g0026871	66.372	226	75	1	357	581	2	227	1.23e-104	315
Msa1331230	MtrunA17_Chr1g0212951	38.117	446	249	5	157	580	13	453	1.42e-103	321
Msa1331230	MtrunA17_Chr4g0030591	37.584	447	252	5	149	578	6	442	1.46e-103	321
Msa1331230	MtrunA17_Chr2g0327721	38.626	422	238	4	174	578	27	444	8.33e-103	319
Msa1331230	MtrunA17_Chr4g0030581	39.716	423	239	3	169	578	22	441	2.76e-102	317
Msa1331230	MtrunA17_Chr4g0026721	66.667	159	53	0	423	581	1	159	1.80e-72	229
Msa1331230	MtrunA17_Chr4g0026791	60.674	178	69	1	357	533	2	179	3.27e-70	224
Msa1331230	MtrunA17_Chr6g0461621	48.718	234	112	4	109	340	2	229	1.97e-65	213
Msa1331230	MtrunA17_Chr4g0026861	48.131	214	109	2	119	330	1	214	3.12e-61	202
Msa1331230	MtrunA17_Chr4g0026801	41.772	158	90	2	119	274	1	158	2.95e-32	122
Msa1331230	MtrunA17_Chr3g0123291	25.000	268	158	8	304	560	154	389	3.71e-12	68.6
Msa1331230	MtrunA17_Chr4g0038581	27.049	244	141	9	313	548	164	378	3.33e-11	65.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1331230	AT2G26640.1	83.865	502	81	0	86	587	8	509	0.0	899
Msa1331230	AT1G04220.1	71.287	505	136	5	91	587	18	521	0.0	747
Msa1331230	AT5G43760.1	72.819	493	128	3	101	587	34	526	0.0	719
Msa1331230	AT2G16280.1	67.276	492	160	1	90	581	20	510	0.0	708
Msa1331230	AT1G19440.1	67.894	489	156	1	91	579	25	512	0.0	707
Msa1331230	AT1G01120.1	63.030	495	177	3	93	581	32	526	0.0	666
Msa1331230	AT1G68530.1	59.759	497	197	2	88	584	1	494	0.0	635
Msa1331230	AT4G34510.1	62.422	479	169	4	106	581	15	485	0.0	630
Msa1331230	AT1G25450.1	59.592	490	197	1	95	584	1	489	0.0	619
Msa1331230	AT2G26250.1	54.757	515	202	5	93	579	37	548	0.0	591
Msa1331230	AT4G34520.1	57.613	486	194	3	107	581	6	490	0.0	570
Msa1331230	AT2G15090.1	59.149	470	181	4	121	581	12	479	0.0	561
Msa1331230	AT4G34250.1	55.992	484	206	3	104	581	5	487	0.0	559
Msa1331230	AT2G46720.1	61.687	415	155	2	170	581	52	465	0.0	549
Msa1331230	AT3G10280.1	60.482	415	153	3	170	581	52	458	0.0	530
Msa1331230	AT1G71160.1	47.406	424	221	2	156	579	25	446	7.76e-144	424
Msa1331230	AT5G49070.1	48.276	435	217	4	157	587	31	461	4.81e-143	422
Msa1331230	AT1G68530.2	53.846	351	160	1	88	438	1	349	3.79e-139	409
Msa1331230	AT3G52160.1	44.332	494	224	8	77	570	7	449	3.28e-138	409
Msa1331230	AT3G52160.2	49.176	364	154	2	207	570	4	336	4.06e-125	372
Msa1331230	AT5G04530.1	40.244	410	235	4	176	578	31	437	3.36e-106	328
Msa1331230	AT2G28630.2	40.046	432	220	7	174	578	27	446	2.75e-105	326
Msa1331230	AT2G28630.1	40.046	432	220	7	174	578	27	446	2.75e-105	326
Msa1331230	AT1G07720.1	40.898	423	230	5	174	578	27	447	2.69e-103	321
Msa1331230	AT1G07720.2	40.898	423	230	5	174	578	70	490	5.38e-103	321
Msa1331230	AT5G49070.2	43.662	284	159	1	157	440	31	313	1.33e-78	251
Msa1331230	AT4G34850.2	25.781	256	152	9	312	558	91	317	3.61e-13	71.2
Msa1331230	AT4G34850.1	25.781	256	152	9	312	558	164	390	1.03e-12	70.5

Find 143 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCACATTGAATTGGTATTT+TGG	0.124443	8_4:+50692075	Msa1331230:CDS
TGCCATTGGCATCTTCTTCT+TGG	0.201240	8_4:-50692260	None:intergenic
GGCAAGTTCCTGGATGAATT+TGG	0.256583	8_4:-50691310	None:intergenic
GGAGCATCTACAATACAATT+TGG	0.261765	8_4:+50691483	Msa1331230:CDS
GTGCACGAAAAGGAAATTTA+TGG	0.264464	8_4:+50691624	Msa1331230:CDS
ACTGGGATCTCTGGTGGGTT+TGG	0.267868	8_4:+50690747	Msa1331230:CDS
TGCCGGAGGTAGAGCTGTTT+TGG	0.290721	8_4:+50692488	Msa1331230:CDS
AGGATGACACTTTATCGTTT+TGG	0.293129	8_4:+50692561	Msa1331230:CDS
TCCCTTCTTAATTCTCCCTT+TGG	0.293276	8_4:-50692631	None:intergenic
ATGACCAATGCATAGGAATT+AGG	0.295997	8_4:-50692039	None:intergenic
ATCAGAATTCCTATATCTTT+AGG	0.301992	8_4:-50691856	None:intergenic
TACCTTGTCAAAAGATCTTA+TGG	0.307989	8_4:+50692293	Msa1331230:CDS
ACAAGTGGCGGGTCTAGTTT+AGG	0.311091	8_4:+50692947	Msa1331230:three_prime_UTR
AGCTTGAAATCAGGAATATA+AGG	0.312020	8_4:-50692441	None:intergenic
TTAACAGATTTCTTGAAATC+AGG	0.316167	8_4:-50691331	None:intergenic
TTTCTAGCTTCTTTCATTGA+AGG	0.316291	8_4:-50691772	None:intergenic
CTTGAAATCAGGCAAGTTCC+TGG	0.317830	8_4:-50691320	None:intergenic
CCACAAGTGGGGAAAGAAAA+AGG	0.317889	8_4:-50691405	None:intergenic
GCTTCTTTCATTGAAGGATT+AGG	0.319754	8_4:-50691766	None:intergenic
CGATAAAGTGTCATCCTTGA+TGG	0.332202	8_4:-50692555	None:intergenic
GGTGGGATGGGTTGCAGTGC+GGG	0.335773	8_4:+50691973	Msa1331230:CDS
TGGCAAATAGCATTTGGTTC+TGG	0.340846	8_4:+50692663	Msa1331230:CDS
GCCATTGGCATCTTCTTCTT+GGG	0.342521	8_4:-50692259	None:intergenic
TCAACAAGGTACACCGGTTT+CGG	0.345198	8_4:-50691568	None:intergenic
GAAGATGCCAATGGCAATAT+TGG	0.352257	8_4:+50692267	Msa1331230:CDS
GTGCACTTACGCGATTCTTC+AGG	0.355117	8_4:-50691607	None:intergenic
CAGTTCACTTTGGTATGAAT+TGG	0.362955	8_4:+50692596	Msa1331230:CDS
TCTGGTGGGTTTGGTCTTCA+AGG	0.363067	8_4:+50690756	Msa1331230:CDS
CAGCATTAGGTGGTAGTTGT+TGG	0.377971	8_4:+50692837	Msa1331230:three_prime_UTR
TTCTAATTGTTTGTTTCGAA+TGG	0.382081	8_4:+50692119	Msa1331230:CDS
CCCAAATATCATAAAAGTCT+TGG	0.400754	8_4:-50691462	None:intergenic
CAAGTGGCGGGTCTAGTTTA+GGG	0.408930	8_4:+50692948	Msa1331230:three_prime_UTR
GTTCAGATGGTAGATACCTT+TGG	0.409315	8_4:-50692789	None:intergenic
ACAGTAAAGCCTAAAGATAT+AGG	0.416419	8_4:+50691847	Msa1331230:CDS
AAAGATCTTATGGCAGTTGC+CGG	0.419635	8_4:+50692303	Msa1331230:CDS
AGAACATGGCAAATAGCATT+TGG	0.419756	8_4:+50692657	Msa1331230:CDS
GATACCTTTGGAACATCAAC+AGG	0.425393	8_4:-50692777	None:intergenic
TTGGAACATCAACAGGAAAC+TGG	0.427742	8_4:-50692770	None:intergenic
TCTCTCTTGGAAGACATTAC+TGG	0.428776	8_4:+50690723	Msa1331230:CDS
AGTAGTTGTGTATCAGCATT+AGG	0.430024	8_4:+50692824	Msa1331230:three_prime_UTR
TCTAATTGTTTGTTTCGAAT+GGG	0.432398	8_4:+50692120	Msa1331230:CDS
TGTTGAATTTAACTTTGTCA+TGG	0.432729	8_4:+50692979	Msa1331230:three_prime_UTR
TGGAGAATATCACATTGAAT+TGG	0.437805	8_4:+50692067	Msa1331230:CDS
TGCGAACCGTGGTGATCAAT+TGG	0.438062	8_4:-50692197	None:intergenic
GCAGCTATCACCATGGCATA+TGG	0.439140	8_4:+50692530	Msa1331230:CDS
TTGAAGATAGCGACGGAGTT+GGG	0.440924	8_4:-50691900	None:intergenic
AAATTGAAGTATGTTAAACT+TGG	0.445451	8_4:+50691352	Msa1331230:CDS
ATTACTGGCACTGGGATCTC+TGG	0.447923	8_4:+50690738	Msa1331230:CDS
CGAAAGATTCTTGAGAGATC+GGG	0.455522	8_4:+50691697	Msa1331230:CDS
TTGGAAGACATTACTGGCAC+TGG	0.465192	8_4:+50690729	Msa1331230:CDS
CTGTTCTTCACCACATTGGT+TGG	0.466086	8_4:+50692390	Msa1331230:CDS
TTCTTGAGAGATCGGGACTC+GGG	0.466115	8_4:+50691704	Msa1331230:CDS
ATTCTTGAGAGATCGGGACT+CGG	0.466508	8_4:+50691703	Msa1331230:CDS
GGAAAGTTAAGAACAGCCTC+TGG	0.468115	8_4:-50691742	None:intergenic
TCAAAAGCTAGCTTGAAATC+AGG	0.468264	8_4:-50692450	None:intergenic
GCGAAAGATTCTTGAGAGAT+CGG	0.468284	8_4:+50691696	Msa1331230:CDS
AACAGTAGTTGTTCTGATGT+TGG	0.477505	8_4:-50692372	None:intergenic
TGTTCTTCACCACATTGGTT+GGG	0.482146	8_4:+50692391	Msa1331230:CDS
GTGCAGTGTGGAAGGCTCTC+AGG	0.489224	8_4:+50692700	Msa1331230:CDS
GTTGAATTTAACTTTGTCAT+GGG	0.489849	8_4:+50692980	Msa1331230:three_prime_UTR
AGCTACAACCTAGGTGGGAT+GGG	0.489907	8_4:+50691961	Msa1331230:CDS
AAAACTAATATCACTACGCT+AGG	0.493178	8_4:+50692336	Msa1331230:CDS
AATTTAACTTTGTCATGGGC+TGG	0.494214	8_4:+50692984	Msa1331230:three_prime_UTR
CTTTCTGGTGTACAAGTGGC+GGG	0.498189	8_4:+50692936	Msa1331230:three_prime_UTR
TATCACTATCTAATCACTCA+TGG	0.499740	8_4:+50691376	Msa1331230:CDS
TTAACTTTGTCATGGGCTGG+TGG	0.502069	8_4:+50692987	Msa1331230:three_prime_UTR
CTGGATGAATTTGGCATCAA+GGG	0.502606	8_4:-50691301	None:intergenic
CTGGCACTGGGATCTCTGGT+GGG	0.510740	8_4:+50690742	Msa1331230:CDS
ACTGGCACTGGGATCTCTGG+TGG	0.512292	8_4:+50690741	Msa1331230:CDS
AGCCAAAGGGAGAATTAAGA+AGG	0.519483	8_4:+50692629	Msa1331230:CDS
CCTGGATGAATTTGGCATCA+AGG	0.520908	8_4:-50691302	None:intergenic
ATTGAAGATAGCGACGGAGT+TGG	0.522625	8_4:-50691901	None:intergenic
GTCAATCATTACAAGCTTCG+AGG	0.523795	8_4:+50691928	Msa1331230:CDS
AATATCATAAAAGTCTTGGA+TGG	0.525987	8_4:-50691458	None:intergenic
CTTACTAAATAGCTCATCAA+TGG	0.525994	8_4:-50691821	None:intergenic
CTATGCATTGGTCATTAGCA+TGG	0.531088	8_4:+50692047	Msa1331230:CDS
GGAAATTTATGGATCAGTCT+AGG	0.532242	8_4:+50691635	Msa1331230:CDS
CATAGGAATTAGGATTGGCC+TGG	0.532315	8_4:-50692029	None:intergenic
ATCCAAAACAGCTCTACCTC+CGG	0.535564	8_4:-50692490	None:intergenic
CAATCCTAATTCCTATGCAT+TGG	0.538699	8_4:+50692035	Msa1331230:CDS
ATTGACTGATCTGTGGATTG+TGG	0.538986	8_4:+50693066	Msa1331230:three_prime_UTR
GCTTTCTGGTGTACAAGTGG+CGG	0.539389	8_4:+50692935	Msa1331230:three_prime_UTR
AATCGCGTAAGTGCACGAAA+AGG	0.539987	8_4:+50691614	Msa1331230:CDS
GTGCAACAGTGCAGTGTGGA+AGG	0.541767	8_4:+50692692	Msa1331230:CDS
GAGCTACAACCTAGGTGGGA+TGG	0.541792	8_4:+50691960	Msa1331230:CDS
CATGGCATATGGAGCCATCA+AGG	0.542439	8_4:+50692541	Msa1331230:CDS
AGGTGGGATGGGTTGCAGTG+CGG	0.542640	8_4:+50691972	Msa1331230:CDS
TCTTTCATTGAAGGATTAGG+AGG	0.545431	8_4:-50691763	None:intergenic
GTGGGATGGGTTGCAGTGCG+GGG	0.548925	8_4:+50691974	Msa1331230:CDS
GTTTCCTGTTGATGTTCCAA+AGG	0.557767	8_4:+50692773	Msa1331230:CDS
ACACATCTAGCAGTTCACTT+TGG	0.560068	8_4:+50692586	Msa1331230:CDS
TGGTCAATCTCATCTATCCA+AGG	0.561417	8_4:-50692750	None:intergenic
GTTTCGAATGGGAGGAGCTG+TGG	0.564123	8_4:+50692131	Msa1331230:CDS
GAAGCTGAGACTGTGATGTT+CGG	0.565891	8_4:+50691796	Msa1331230:CDS
TCAATCATTACAAGCTTCGA+GGG	0.569665	8_4:+50691929	Msa1331230:CDS
ACTACTGTTCTTCACCACAT+TGG	0.569759	8_4:+50692386	Msa1331230:CDS
AATTGTTTGTTTCGAATGGG+AGG	0.571309	8_4:+50692123	Msa1331230:CDS
AAATTGGAAAACATGGGAAG+AGG	0.571781	8_4:-50690820	None:intergenic
TAACTTTGTCATGGGCTGGT+GGG	0.573556	8_4:+50692988	Msa1331230:three_prime_UTR
AATATAAAGAGCTACAACCT+AGG	0.575611	8_4:+50691952	Msa1331230:CDS
CAGGACAATCAACCCTGTGA+AGG	0.575639	8_4:+50692719	Msa1331230:CDS
TGTATTTATTGACTGATCTG+TGG	0.577430	8_4:+50693059	Msa1331230:three_prime_UTR
TCTGCTTTCTGGTGTACAAG+TGG	0.577609	8_4:+50692932	Msa1331230:three_prime_UTR
TTACAATCTCGTCGTTCAGA+TGG	0.578695	8_4:-50692802	None:intergenic
TCTTGCTAAAGATCTTCTCC+AGG	0.579124	8_4:+50692011	Msa1331230:CDS
GCAGCAATCAACACCACAAG+TGG	0.584783	8_4:-50691418	None:intergenic
AAGTGGCGGGTCTAGTTTAG+GGG	0.585270	8_4:+50692949	Msa1331230:three_prime_UTR
TAAAGAGCTACAACCTAGGT+GGG	0.586492	8_4:+50691956	Msa1331230:CDS
TGGCTTACACAGAAGCCAAA+GGG	0.588822	8_4:+50692616	Msa1331230:CDS
GGTAACACCAATATTGCCAT+TGG	0.592052	8_4:-50692274	None:intergenic
AAACAGCTCTACCTCCGGCA+TGG	0.599316	8_4:-50692485	None:intergenic
CCTTGATGCCAAATTCATCC+AGG	0.603328	8_4:+50691302	Msa1331230:CDS
CAATGCATAGGAATTAGGAT+TGG	0.604720	8_4:-50692034	None:intergenic
TGGAAGACATTACTGGCACT+GGG	0.608170	8_4:+50690730	Msa1331230:CDS
CACCACGGTTCGCACTAACA+AGG	0.615650	8_4:+50692206	Msa1331230:CDS
TCTTGAGAGATCGGGACTCG+GGG	0.615846	8_4:+50691705	Msa1331230:CDS
GCCAAAGGGAGAATTAAGAA+GGG	0.617206	8_4:+50692630	Msa1331230:CDS
CATGCTAATGACCAATGCAT+AGG	0.618448	8_4:-50692046	None:intergenic
TATATCTTTAGGCTTTACTG+TGG	0.620510	8_4:-50691845	None:intergenic
TTGGCTTACACAGAAGCCAA+AGG	0.624548	8_4:+50692615	Msa1331230:CDS
CTGTGAAGGAGAAAAGCCCT+TGG	0.625413	8_4:+50692733	Msa1331230:CDS
AAACTAATATCACTACGCTA+GGG	0.626894	8_4:+50692337	Msa1331230:CDS
GGTCAATCTCATCTATCCAA+GGG	0.627776	8_4:-50692749	None:intergenic
CAGCAATCAACACCACAAGT+GGG	0.638117	8_4:-50691417	None:intergenic
CACTGCAACCCATCCCACCT+AGG	0.640569	8_4:-50691969	None:intergenic
ATAAAGAGCTACAACCTAGG+TGG	0.642158	8_4:+50691955	Msa1331230:CDS
TCTGATGTTGGAAGTACGAG+AGG	0.642235	8_4:-50692360	None:intergenic
GGAATCTACTTATCTTCCAG+AGG	0.644232	8_4:+50691726	Msa1331230:CDS
TTAAGAAGGGAGATAGAACA+TGG	0.646961	8_4:+50692643	Msa1331230:CDS
AAAGCGTGGACAGGAAAACA+AGG	0.654849	8_4:-50691531	None:intergenic
AGCAATCAACACCACAAGTG+GGG	0.662251	8_4:-50691416	None:intergenic
ACCCAAGAAGAAGATGCCAA+TGG	0.668165	8_4:+50692258	Msa1331230:CDS
AAGTTTCTTCCCAACCAATG+TGG	0.668173	8_4:-50692400	None:intergenic
ACAATCATTGAAGATAGCGA+CGG	0.668618	8_4:-50691907	None:intergenic
CTTGATGGCTCCATATGCCA+TGG	0.676445	8_4:-50692540	None:intergenic
GAGAAATCAACAAGGTACAC+CGG	0.678354	8_4:-50691574	None:intergenic
AGTTGTGTATCAGCATTAGG+TGG	0.680421	8_4:+50692827	Msa1331230:three_prime_UTR
GTGTGTGTGTGCGCGCAAGG+AGG	0.690913	8_4:-50690686	None:intergenic
TCAAGTGCAACAGTGCAGTG+TGG	0.695312	8_4:+50692688	Msa1331230:CDS
AAAACTTGCAGCTATCACCA+TGG	0.706018	8_4:+50692523	Msa1331230:CDS
AGCCTTGTTAGTGCGAACCG+TGG	0.715624	8_4:-50692208	None:intergenic
AAAATACCAATTGATCACCA+CGG	0.722409	8_4:+50692191	Msa1331230:CDS
TGTAACATGAGAAATCAACA+AGG	0.794037	8_4:-50691582	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTAATAATCAACAAAAA+TGG	-	chr8_4:50691063-50691082	None:intergenic	10.0%
!!	ATTGTTTAAGAAAAATATGA+AGG	-	chr8_4:50690663-50690682	None:intergenic	15.0%
!!!	GATTACATATTTTCTTTTAA+TGG	-	chr8_4:50691222-50691241	None:intergenic	15.0%
!!	AATGAAAAATTGGAAAACAT+GGG	-	chr8_4:50690829-50690848	None:intergenic	20.0%
!!	TAATGAAAAATTGGAAAACA+TGG	-	chr8_4:50690830-50690849	None:intergenic	20.0%
!!!	ATTGATTTAACTTTTGAACA+GGG	+	chr8_4:50691249-50691268	Msa1331230:intron	20.0%
!!	AAATTGAAGTATGTTAAACT+TGG	+	chr8_4:50691352-50691371	Msa1331230:CDS	20.0%
!!!	ATTGATGAAATTTTCTTTCT+GGG	+	chr8_4:50692860-50692879	Msa1331230:three_prime_UTR	20.0%
!	GTTAATTTACATGCATTGAA+TGG	-	chr8_4:50691041-50691060	None:intergenic	25.0%
!	GTAGAAATTGTTACTGAAAA+TGG	+	chr8_4:50691095-50691114	Msa1331230:intron	25.0%
!!!	GATTGATTTAACTTTTGAAC+AGG	+	chr8_4:50691248-50691267	Msa1331230:intron	25.0%
!	TTAACAGATTTCTTGAAATC+AGG	-	chr8_4:50691334-50691353	None:intergenic	25.0%
!	AATATCATAAAAGTCTTGGA+TGG	-	chr8_4:50691461-50691480	None:intergenic	25.0%
!!	TCCAAGACTTTTATGATATT+TGG	+	chr8_4:50691461-50691480	Msa1331230:CDS	25.0%
!!	CCAAGACTTTTATGATATTT+GGG	+	chr8_4:50691462-50691481	Msa1331230:CDS	25.0%
!	ATCAGAATTCCTATATCTTT+AGG	-	chr8_4:50691859-50691878	None:intergenic	25.0%
!!	ATCACATTGAATTGGTATTT+TGG	+	chr8_4:50692075-50692094	Msa1331230:CDS	25.0%
!	TTCTAATTGTTTGTTTCGAA+TGG	+	chr8_4:50692119-50692138	Msa1331230:CDS	25.0%
!	TCTAATTGTTTGTTTCGAAT+GGG	+	chr8_4:50692120-50692139	Msa1331230:CDS	25.0%
!!!	ATTAGTTTTTAAAGCATCAC+CGG	-	chr8_4:50692325-50692344	None:intergenic	25.0%
!!	GATTGATGAAATTTTCTTTC+TGG	+	chr8_4:50692859-50692878	Msa1331230:three_prime_UTR	25.0%
!!	TGTTGAATTTAACTTTGTCA+TGG	+	chr8_4:50692979-50692998	Msa1331230:three_prime_UTR	25.0%
!!	GTTGAATTTAACTTTGTCAT+GGG	+	chr8_4:50692980-50692999	Msa1331230:three_prime_UTR	25.0%
!	GAAAAATCACAGTTAAAATC+TGG	-	chr8_4:50693123-50693142	None:intergenic	25.0%
!	AAGGTACAATTTTGTGAACT+GGG	+	chr8_4:50690775-50690794	Msa1331230:CDS	30.0%
	TGCAGTGAGTAATGAAAAAT+TGG	-	chr8_4:50690839-50690858	None:intergenic	30.0%
	CATTGTGGATTTATACACAA+AGG	+	chr8_4:50690913-50690932	Msa1331230:intron	30.0%
	TACTCAACATGAAAATCAAG+TGG	-	chr8_4:50691018-50691037	None:intergenic	30.0%
!	GTTACTGAAAATGGATCTTT+TGG	+	chr8_4:50691104-50691123	Msa1331230:intron	30.0%
	AATCATGAGCAACAACAAAA+GGG	-	chr8_4:50691196-50691215	None:intergenic	30.0%
	AAATCATGAGCAACAACAAA+AGG	-	chr8_4:50691197-50691216	None:intergenic	30.0%
	TATCACTATCTAATCACTCA+TGG	+	chr8_4:50691376-50691395	Msa1331230:CDS	30.0%
	CCCAAATATCATAAAAGTCT+TGG	-	chr8_4:50691465-50691484	None:intergenic	30.0%
	TGTAACATGAGAAATCAACA+AGG	-	chr8_4:50691585-50691604	None:intergenic	30.0%
!	TAGGATGATCAGTACTTTTA+CGG	+	chr8_4:50691654-50691673	Msa1331230:CDS	30.0%
!!	TTTCTAGCTTCTTTCATTGA+AGG	-	chr8_4:50691775-50691794	None:intergenic	30.0%
	CTTACTAAATAGCTCATCAA+TGG	-	chr8_4:50691824-50691843	None:intergenic	30.0%
	TATATCTTTAGGCTTTACTG+TGG	-	chr8_4:50691848-50691867	None:intergenic	30.0%
	ACAGTAAAGCCTAAAGATAT+AGG	+	chr8_4:50691847-50691866	Msa1331230:CDS	30.0%
	AATATAAAGAGCTACAACCT+AGG	+	chr8_4:50691952-50691971	Msa1331230:CDS	30.0%
	TGGAGAATATCACATTGAAT+TGG	+	chr8_4:50692067-50692086	Msa1331230:CDS	30.0%
!!	ATTGGTATTTTGGAAACGAT+CGG	+	chr8_4:50692085-50692104	Msa1331230:CDS	30.0%
	AAAATACCAATTGATCACCA+CGG	+	chr8_4:50692191-50692210	Msa1331230:CDS	30.0%
	TACCTTGTCAAAAGATCTTA+TGG	+	chr8_4:50692293-50692312	Msa1331230:CDS	30.0%
	AAAACTAATATCACTACGCT+AGG	+	chr8_4:50692336-50692355	Msa1331230:CDS	30.0%
	AAACTAATATCACTACGCTA+GGG	+	chr8_4:50692337-50692356	Msa1331230:CDS	30.0%
	AGCTTGAAATCAGGAATATA+AGG	-	chr8_4:50692444-50692463	None:intergenic	30.0%
!	AGGAATTTTATTCTGCTTTC+TGG	+	chr8_4:50692921-50692940	Msa1331230:three_prime_UTR	30.0%
!	TGTATTTATTGACTGATCTG+TGG	+	chr8_4:50693059-50693078	Msa1331230:three_prime_UTR	30.0%
!	CAAGGTACAATTTTGTGAAC+TGG	+	chr8_4:50690774-50690793	Msa1331230:CDS	35.0%
	AAATTGGAAAACATGGGAAG+AGG	-	chr8_4:50690823-50690842	None:intergenic	35.0%
!	ACTGAAAATGGATCTTTTGG+AGG	+	chr8_4:50691107-50691126	Msa1331230:intron	35.0%
!	CTGAAAATGGATCTTTTGGA+GGG	+	chr8_4:50691108-50691127	Msa1331230:intron	35.0%
	AACAGGGCAAAAATGACAAA+TGG	+	chr8_4:50691265-50691284	Msa1331230:intron	35.0%
	AAAGTCTTGGATGGAAAAAG+TGG	-	chr8_4:50691452-50691471	None:intergenic	35.0%
!	GGAGCATCTACAATACAATT+TGG	+	chr8_4:50691483-50691502	Msa1331230:CDS	35.0%
	GTGCACGAAAAGGAAATTTA+TGG	+	chr8_4:50691624-50691643	Msa1331230:CDS	35.0%
	GGAAATTTATGGATCAGTCT+AGG	+	chr8_4:50691635-50691654	Msa1331230:CDS	35.0%
	TCTTTCATTGAAGGATTAGG+AGG	-	chr8_4:50691766-50691785	None:intergenic	35.0%
	GCTTCTTTCATTGAAGGATT+AGG	-	chr8_4:50691769-50691788	None:intergenic	35.0%
	ACAATCATTGAAGATAGCGA+CGG	-	chr8_4:50691910-50691929	None:intergenic	35.0%
	TCAATCATTACAAGCTTCGA+GGG	+	chr8_4:50691929-50691948	Msa1331230:CDS	35.0%
	CAATGCATAGGAATTAGGAT+TGG	-	chr8_4:50692037-50692056	None:intergenic	35.0%
	CAATCCTAATTCCTATGCAT+TGG	+	chr8_4:50692035-50692054	Msa1331230:CDS	35.0%
	ATGACCAATGCATAGGAATT+AGG	-	chr8_4:50692042-50692061	None:intergenic	35.0%
	AATTGTTTGTTTCGAATGGG+AGG	+	chr8_4:50692123-50692142	Msa1331230:CDS	35.0%
!!	TCTTCTGTCTGAGGTTTTAT+TGG	-	chr8_4:50692166-50692185	None:intergenic	35.0%
!	TTTTGATCTTCTTCTGTCTG+AGG	-	chr8_4:50692175-50692194	None:intergenic	35.0%
!	TGCCATAAGATCTTTTGACA+AGG	-	chr8_4:50692298-50692317	None:intergenic	35.0%
	AACAGTAGTTGTTCTGATGT+TGG	-	chr8_4:50692375-50692394	None:intergenic	35.0%
!	TCAAAAGCTAGCTTGAAATC+AGG	-	chr8_4:50692453-50692472	None:intergenic	35.0%
!	AGGATGACACTTTATCGTTT+TGG	+	chr8_4:50692561-50692580	Msa1331230:CDS	35.0%
	CAGTTCACTTTGGTATGAAT+TGG	+	chr8_4:50692596-50692615	Msa1331230:CDS	35.0%
	TTAAGAAGGGAGATAGAACA+TGG	+	chr8_4:50692643-50692662	Msa1331230:CDS	35.0%
	AGAACATGGCAAATAGCATT+TGG	+	chr8_4:50692657-50692676	Msa1331230:CDS	35.0%
	AGTAGTTGTGTATCAGCATT+AGG	+	chr8_4:50692824-50692843	Msa1331230:three_prime_UTR	35.0%
!!!	GTTTTTCGAGTTCAGGTTTT+AGG	+	chr8_4:50692901-50692920	Msa1331230:three_prime_UTR	35.0%
!	AATTTAACTTTGTCATGGGC+TGG	+	chr8_4:50692984-50693003	Msa1331230:three_prime_UTR	35.0%
	TCTCTCTTGGAAGACATTAC+TGG	+	chr8_4:50690723-50690742	Msa1331230:CDS	40.0%
	AATCCACAATGCATTCGTTC+AGG	-	chr8_4:50690904-50690923	None:intergenic	40.0%
	CTGGATGAATTTGGCATCAA+GGG	-	chr8_4:50691304-50691323	None:intergenic	40.0%
	TCAAAAAGTAAAGCGTGGAC+AGG	-	chr8_4:50691543-50691562	None:intergenic	40.0%
	ACGAGTCAAAAAGTAAAGCG+TGG	-	chr8_4:50691548-50691567	None:intergenic	40.0%
	GAGAAATCAACAAGGTACAC+CGG	-	chr8_4:50691577-50691596	None:intergenic	40.0%
!	GATGATCAGTACTTTTACGG+AGG	+	chr8_4:50691657-50691676	Msa1331230:CDS	40.0%
	GCGAAAGATTCTTGAGAGAT+CGG	+	chr8_4:50691696-50691715	Msa1331230:CDS	40.0%
	CGAAAGATTCTTGAGAGATC+GGG	+	chr8_4:50691697-50691716	Msa1331230:CDS	40.0%
	GGAATCTACTTATCTTCCAG+AGG	+	chr8_4:50691726-50691745	Msa1331230:CDS	40.0%
	GTCAATCATTACAAGCTTCG+AGG	+	chr8_4:50691928-50691947	Msa1331230:CDS	40.0%
	ATAAAGAGCTACAACCTAGG+TGG	+	chr8_4:50691955-50691974	Msa1331230:CDS	40.0%
	TAAAGAGCTACAACCTAGGT+GGG	+	chr8_4:50691956-50691975	Msa1331230:CDS	40.0%
	TCTTGCTAAAGATCTTCTCC+AGG	+	chr8_4:50692011-50692030	Msa1331230:CDS	40.0%
	CATGCTAATGACCAATGCAT+AGG	-	chr8_4:50692049-50692068	None:intergenic	40.0%
!	CTATGCATTGGTCATTAGCA+TGG	+	chr8_4:50692047-50692066	Msa1331230:CDS	40.0%
	GAAGATGCCAATGGCAATAT+TGG	+	chr8_4:50692267-50692286	Msa1331230:CDS	40.0%
	GGTAACACCAATATTGCCAT+TGG	-	chr8_4:50692277-50692296	None:intergenic	40.0%
	AAAGATCTTATGGCAGTTGC+CGG	+	chr8_4:50692303-50692322	Msa1331230:CDS	40.0%
	ACTACTGTTCTTCACCACAT+TGG	+	chr8_4:50692386-50692405	Msa1331230:CDS	40.0%
!	TGTTCTTCACCACATTGGTT+GGG	+	chr8_4:50692391-50692410	Msa1331230:CDS	40.0%
	AAGTTTCTTCCCAACCAATG+TGG	-	chr8_4:50692403-50692422	None:intergenic	40.0%
!	GAACATTTTTGCATCCATGC+CGG	+	chr8_4:50692471-50692490	Msa1331230:CDS	40.0%
!!	TAGAGCTGTTTTGGATGAAC+TGG	+	chr8_4:50692497-50692516	Msa1331230:CDS	40.0%
	AAAACTTGCAGCTATCACCA+TGG	+	chr8_4:50692523-50692542	Msa1331230:CDS	40.0%
!	CGATAAAGTGTCATCCTTGA+TGG	-	chr8_4:50692558-50692577	None:intergenic	40.0%
	ACACATCTAGCAGTTCACTT+TGG	+	chr8_4:50692586-50692605	Msa1331230:CDS	40.0%
	AGCCAAAGGGAGAATTAAGA+AGG	+	chr8_4:50692629-50692648	Msa1331230:CDS	40.0%
	GCCAAAGGGAGAATTAAGAA+GGG	+	chr8_4:50692630-50692649	Msa1331230:CDS	40.0%
	TCCCTTCTTAATTCTCCCTT+TGG	-	chr8_4:50692634-50692653	None:intergenic	40.0%
!	TGGCAAATAGCATTTGGTTC+TGG	+	chr8_4:50692663-50692682	Msa1331230:CDS	40.0%
	GGTCAATCTCATCTATCCAA+GGG	-	chr8_4:50692752-50692771	None:intergenic	40.0%
	TGGTCAATCTCATCTATCCA+AGG	-	chr8_4:50692753-50692772	None:intergenic	40.0%
	TTGGAACATCAACAGGAAAC+TGG	-	chr8_4:50692773-50692792	None:intergenic	40.0%
	GTTTCCTGTTGATGTTCCAA+AGG	+	chr8_4:50692773-50692792	Msa1331230:CDS	40.0%
!!	GATACCTTTGGAACATCAAC+AGG	-	chr8_4:50692780-50692799	None:intergenic	40.0%
	GTTCAGATGGTAGATACCTT+TGG	-	chr8_4:50692792-50692811	None:intergenic	40.0%
	TTACAATCTCGTCGTTCAGA+TGG	-	chr8_4:50692805-50692824	None:intergenic	40.0%
	AGTTGTGTATCAGCATTAGG+TGG	+	chr8_4:50692827-50692846	Msa1331230:three_prime_UTR	40.0%
!!	CTTGTGATTGTTTTGCTGTG+AGG	+	chr8_4:50693020-50693039	Msa1331230:three_prime_UTR	40.0%
!	ATTGACTGATCTGTGGATTG+TGG	+	chr8_4:50693066-50693085	Msa1331230:three_prime_UTR	40.0%
	TTGGAAGACATTACTGGCAC+TGG	+	chr8_4:50690729-50690748	Msa1331230:CDS	45.0%
	TGGAAGACATTACTGGCACT+GGG	+	chr8_4:50690730-50690749	Msa1331230:CDS	45.0%
	CTTCCTGAACGAATGCATTG+TGG	+	chr8_4:50690898-50690917	Msa1331230:intron	45.0%
	CCTGGATGAATTTGGCATCA+AGG	-	chr8_4:50691305-50691324	None:intergenic	45.0%
	CCTTGATGCCAAATTCATCC+AGG	+	chr8_4:50691302-50691321	Msa1331230:CDS	45.0%
	GGCAAGTTCCTGGATGAATT+TGG	-	chr8_4:50691313-50691332	None:intergenic	45.0%
	CTTGAAATCAGGCAAGTTCC+TGG	-	chr8_4:50691323-50691342	None:intergenic	45.0%
	CCACAAGTGGGGAAAGAAAA+AGG	-	chr8_4:50691408-50691427	None:intergenic	45.0%
!	CCTTTTTCTTTCCCCACTTG+TGG	+	chr8_4:50691405-50691424	Msa1331230:CDS	45.0%
	AGCAATCAACACCACAAGTG+GGG	-	chr8_4:50691419-50691438	None:intergenic	45.0%
	CAGCAATCAACACCACAAGT+GGG	-	chr8_4:50691420-50691439	None:intergenic	45.0%
	AAAGCGTGGACAGGAAAACA+AGG	-	chr8_4:50691534-50691553	None:intergenic	45.0%
!!	CTTTTTGACTCGTCCGAAAC+CGG	+	chr8_4:50691555-50691574	Msa1331230:CDS	45.0%
!!	TCAACAAGGTACACCGGTTT+CGG	-	chr8_4:50691571-50691590	None:intergenic	45.0%
	AATCGCGTAAGTGCACGAAA+AGG	+	chr8_4:50691614-50691633	Msa1331230:CDS	45.0%
	ATTCTTGAGAGATCGGGACT+CGG	+	chr8_4:50691703-50691722	Msa1331230:CDS	45.0%
	GGAAAGTTAAGAACAGCCTC+TGG	-	chr8_4:50691745-50691764	None:intergenic	45.0%
	GAAGCTGAGACTGTGATGTT+CGG	+	chr8_4:50691796-50691815	Msa1331230:CDS	45.0%
	TTGAAGATAGCGACGGAGTT+GGG	-	chr8_4:50691903-50691922	None:intergenic	45.0%
	ATTGAAGATAGCGACGGAGT+TGG	-	chr8_4:50691904-50691923	None:intergenic	45.0%
!!	CATAGGAATTAGGATTGGCC+TGG	-	chr8_4:50692032-50692051	None:intergenic	45.0%
	ACCCAAGAAGAAGATGCCAA+TGG	+	chr8_4:50692258-50692277	Msa1331230:CDS	45.0%
	GCCATTGGCATCTTCTTCTT+GGG	-	chr8_4:50692262-50692281	None:intergenic	45.0%
	TGCCATTGGCATCTTCTTCT+TGG	-	chr8_4:50692263-50692282	None:intergenic	45.0%
!	TCTGATGTTGGAAGTACGAG+AGG	-	chr8_4:50692363-50692382	None:intergenic	45.0%
	CTGTTCTTCACCACATTGGT+TGG	+	chr8_4:50692390-50692409	Msa1331230:CDS	45.0%
	ATCCAAAACAGCTCTACCTC+CGG	-	chr8_4:50692493-50692512	None:intergenic	45.0%
	TTGGCTTACACAGAAGCCAA+AGG	+	chr8_4:50692615-50692634	Msa1331230:CDS	45.0%
	TGGCTTACACAGAAGCCAAA+GGG	+	chr8_4:50692616-50692635	Msa1331230:CDS	45.0%
!	AAGGGCTTTTCTCCTTCACA+GGG	-	chr8_4:50692734-50692753	None:intergenic	45.0%
	CAGCATTAGGTGGTAGTTGT+TGG	+	chr8_4:50692837-50692856	Msa1331230:three_prime_UTR	45.0%
!!!	CGTGACTGTTTTTCGAGTTC+AGG	+	chr8_4:50692894-50692913	Msa1331230:three_prime_UTR	45.0%
!	TCTGCTTTCTGGTGTACAAG+TGG	+	chr8_4:50692932-50692951	Msa1331230:three_prime_UTR	45.0%
!!	TTAACTTTGTCATGGGCTGG+TGG	+	chr8_4:50692987-50693006	Msa1331230:three_prime_UTR	45.0%
!!	TAACTTTGTCATGGGCTGGT+GGG	+	chr8_4:50692988-50693007	Msa1331230:three_prime_UTR	45.0%
!	GCTGTGAGGTGTATTTTCTC+AGG	+	chr8_4:50693034-50693053	Msa1331230:three_prime_UTR	45.0%
	CACTCTCTCTCTCTCTCTCT+TGG	+	chr8_4:50690710-50690729	Msa1331230:CDS	50.0%
!	ATTACTGGCACTGGGATCTC+TGG	+	chr8_4:50690738-50690757	Msa1331230:CDS	50.0%
!!	TCTGGTGGGTTTGGTCTTCA+AGG	+	chr8_4:50690756-50690775	Msa1331230:CDS	50.0%
	GCAGCAATCAACACCACAAG+TGG	-	chr8_4:50691421-50691440	None:intergenic	50.0%
	GTGCACTTACGCGATTCTTC+AGG	-	chr8_4:50691610-50691629	None:intergenic	50.0%
	TTCTTGAGAGATCGGGACTC+GGG	+	chr8_4:50691704-50691723	Msa1331230:CDS	50.0%
	AGCTACAACCTAGGTGGGAT+GGG	+	chr8_4:50691961-50691980	Msa1331230:CDS	50.0%
	TGCGAACCGTGGTGATCAAT+TGG	-	chr8_4:50692200-50692219	None:intergenic	50.0%
!	CATTTTTGCATCCATGCCGG+AGG	+	chr8_4:50692474-50692493	Msa1331230:CDS	50.0%
	GCAGCTATCACCATGGCATA+TGG	+	chr8_4:50692530-50692549	Msa1331230:CDS	50.0%
!	CTTGATGGCTCCATATGCCA+TGG	-	chr8_4:50692543-50692562	None:intergenic	50.0%
	CATGGCATATGGAGCCATCA+AGG	+	chr8_4:50692541-50692560	Msa1331230:CDS	50.0%
	TCAAGTGCAACAGTGCAGTG+TGG	+	chr8_4:50692688-50692707	Msa1331230:CDS	50.0%
	CAGGACAATCAACCCTGTGA+AGG	+	chr8_4:50692719-50692738	Msa1331230:CDS	50.0%
!	CAAGGGCTTTTCTCCTTCAC+AGG	-	chr8_4:50692735-50692754	None:intergenic	50.0%
	CTGTGAAGGAGAAAAGCCCT+TGG	+	chr8_4:50692733-50692752	Msa1331230:CDS	50.0%
!	GCTTTCTGGTGTACAAGTGG+CGG	+	chr8_4:50692935-50692954	Msa1331230:three_prime_UTR	50.0%
!	CTTTCTGGTGTACAAGTGGC+GGG	+	chr8_4:50692936-50692955	Msa1331230:three_prime_UTR	50.0%
!!	ACAAGTGGCGGGTCTAGTTT+AGG	+	chr8_4:50692947-50692966	Msa1331230:three_prime_UTR	50.0%
!!	CAAGTGGCGGGTCTAGTTTA+GGG	+	chr8_4:50692948-50692967	Msa1331230:three_prime_UTR	50.0%
!!	AAGTGGCGGGTCTAGTTTAG+GGG	+	chr8_4:50692949-50692968	Msa1331230:three_prime_UTR	50.0%
!!	ACTGGGATCTCTGGTGGGTT+TGG	+	chr8_4:50690747-50690766	Msa1331230:CDS	55.0%
	TCTTGAGAGATCGGGACTCG+GGG	+	chr8_4:50691705-50691724	Msa1331230:CDS	55.0%
	GAGCTACAACCTAGGTGGGA+TGG	+	chr8_4:50691960-50691979	Msa1331230:CDS	55.0%
	GTTTCGAATGGGAGGAGCTG+TGG	+	chr8_4:50692131-50692150	Msa1331230:CDS	55.0%
	CACCACGGTTCGCACTAACA+AGG	+	chr8_4:50692206-50692225	Msa1331230:CDS	55.0%
!!	AGCCTTGTTAGTGCGAACCG+TGG	-	chr8_4:50692211-50692230	None:intergenic	55.0%
	AAACAGCTCTACCTCCGGCA+TGG	-	chr8_4:50692488-50692507	None:intergenic	55.0%
!	TGCCGGAGGTAGAGCTGTTT+TGG	+	chr8_4:50692488-50692507	Msa1331230:CDS	55.0%
	GTGCAACAGTGCAGTGTGGA+AGG	+	chr8_4:50692692-50692711	Msa1331230:CDS	55.0%
	TGTGTGTGTGTGTGCGCGCA+AGG	-	chr8_4:50690692-50690711	None:intergenic	60.0%
!	ACTGGCACTGGGATCTCTGG+TGG	+	chr8_4:50690741-50690760	Msa1331230:CDS	60.0%
!	CTGGCACTGGGATCTCTGGT+GGG	+	chr8_4:50690742-50690761	Msa1331230:CDS	60.0%
	CACTGCAACCCATCCCACCT+AGG	-	chr8_4:50691972-50691991	None:intergenic	60.0%
!!	AGGTGGGATGGGTTGCAGTG+CGG	+	chr8_4:50691972-50691991	Msa1331230:CDS	60.0%
	GTGCAGTGTGGAAGGCTCTC+AGG	+	chr8_4:50692700-50692719	Msa1331230:CDS	60.0%
	GTGTGTGTGTGCGCGCAAGG+AGG	-	chr8_4:50690689-50690708	None:intergenic	65.0%
!!	GGTGGGATGGGTTGCAGTGC+GGG	+	chr8_4:50691973-50691992	Msa1331230:CDS	65.0%
!!	GTGGGATGGGTTGCAGTGCG+GGG	+	chr8_4:50691974-50691993	Msa1331230:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	50690656	50693159	50690656	ID=Msa1331230;Name=Msa1331230
chr8_4	mRNA	50690656	50693159	50690656	ID=Msa1331230-mRNA-1;Parent=Msa1331230;Name=Msa1331230-mRNA-1;_AED=0.02;_eAED=0.11;_QI=0\|0\|0.5\|1\|0\|0\|2\|350\|587
chr8_4	exon	50690656	50690879	50690656	ID=Msa1331230-mRNA-1:exon:14852;Parent=Msa1331230-mRNA-1
chr8_4	exon	50691270	50693159	50691270	ID=Msa1331230-mRNA-1:exon:14853;Parent=Msa1331230-mRNA-1
chr8_4	CDS	50690656	50690879	50690656	ID=Msa1331230-mRNA-1:cds;Parent=Msa1331230-mRNA-1
chr8_4	CDS	50691270	50692809	50691270	ID=Msa1331230-mRNA-1:cds;Parent=Msa1331230-mRNA-1
chr8_4	three_prime_UTR	50692810	50693159	50692810	ID=Msa1331230-mRNA-1:three_prime_utr;Parent=Msa1331230-mRNA-1

Gene Sequence

>Msa1331230

ATGTCCTTCATATTTTTCTTAAACAATTATCCTCCTTGCGCGCACACACACACACACTCTCTCTCTCTCTCTCTTGGAAGACATTACTGGCACTGGGATCTCTGGTGGGTTTGGTCTTCAAGGTACAATTTTGTGAACTGGGTTTTGTTTCACATCAACATTAACCTCTTCCCATGTTTTCCAATTTTTCATTACTCACTGCATGCTTCTTCTTTGTTTCACTTGGCAAAAATGACAAATGGTAGATTAGATACACCCTTGATGCCAAATTCATCCAGGAACTTGCCTGATTTCAAGAAATCTGTTAAATTGAAGTATGTTAAACTTGGTTATCACTATCTAATCACTCATGGAATGTACCTTTTTCTTTCCCCACTTGTGGTGTTGATTGCTGCTCAGATATCCACTTTTTCCATCCAAGACTTTTATGATATTTGGGAGCATCTACAATACAATTTGGTATCTGTCGTTTTTTGTTCAACTCTCCTTGTTTTCCTGTCCACGCTTTACTTTTTGACTCGTCCGAAACCGGTGTACCTTGTTGATTTCTCATGTTACAAGCCTGAAGAATCGCGTAAGTGCACGAAAAGGAAATTTATGGATCAGTCTAGGATGATCAGTACTTTTACGGAGGAAAATCTTGAATTTCAGCGAAAGATTCTTGAGAGATCGGGACTCGGGGAATCTACTTATCTTCCAGAGGCTGTTCTTAACTTTCCTCCTAATCCTTCAATGAAAGAAGCTAGAAAAGAAGCTGAGACTGTGATGTTCGGTGCCATTGATGAGCTATTTAGTAAGACCACAGTAAAGCCTAAAGATATAGGAATTCTGATTGTAAATTGTAGCTTGTTCAACCCAACTCCGTCGCTATCTTCAATGATTGTCAATCATTACAAGCTTCGAGGGAATATAAAGAGCTACAACCTAGGTGGGATGGGTTGCAGTGCGGGGCTGATCTCGATTGATCTTGCTAAAGATCTTCTCCAGGCCAATCCTAATTCCTATGCATTGGTCATTAGCATGGAGAATATCACATTGAATTGGTATTTTGGAAACGATCGGTCGAAACTTGTTTCTAATTGTTTGTTTCGAATGGGAGGAGCTGTGGTTCTGCTTTCCAATAAAACCTCAGACAGAAGAAGATCAAAATACCAATTGATCACCACGGTTCGCACTAACAAGGCTTCTGATGACAAGTGTTTTAGCTGTGTAACCCAAGAAGAAGATGCCAATGGCAATATTGGTGTTACCTTGTCAAAAGATCTTATGGCAGTTGCCGGTGATGCTTTAAAAACTAATATCACTACGCTAGGGCCTCTCGTACTTCCAACATCAGAACAACTACTGTTCTTCACCACATTGGTTGGGAAGAAACTTTTCAAGATGAAGATAAAACCTTATATTCCTGATTTCAAGCTAGCTTTTGAACATTTTTGCATCCATGCCGGAGGTAGAGCTGTTTTGGATGAACTGGAGAAAAACTTGCAGCTATCACCATGGCATATGGAGCCATCAAGGATGACACTTTATCGTTTTGGAAACACATCTAGCAGTTCACTTTGGTATGAATTGGCTTACACAGAAGCCAAAGGGAGAATTAAGAAGGGAGATAGAACATGGCAAATAGCATTTGGTTCTGGATTCAAGTGCAACAGTGCAGTGTGGAAGGCTCTCAGGACAATCAACCCTGTGAAGGAGAAAAGCCCTTGGATAGATGAGATTGACCAGTTTCCTGTTGATGTTCCAAAGGTATCTACCATCTGA

Protein sequence

>Msa1331230

MSFIFFLNNYPPCAHTHTHSLSLSLGRHYWHWDLWWVWSSRYNFVNWVLFHININLFPCFPIFHYSLHASSLFHLAKMTNGRLDTPLMPNSSRNLPDFKKSVKLKYVKLGYHYLITHGMYLFLSPLVVLIAAQISTFSIQDFYDIWEHLQYNLVSVVFCSTLLVFLSTLYFLTRPKPVYLVDFSCYKPEESRKCTKRKFMDQSRMISTFTEENLEFQRKILERSGLGESTYLPEAVLNFPPNPSMKEARKEAETVMFGAIDELFSKTTVKPKDIGILIVNCSLFNPTPSLSSMIVNHYKLRGNIKSYNLGGMGCSAGLISIDLAKDLLQANPNSYALVISMENITLNWYFGNDRSKLVSNCLFRMGGAVVLLSNKTSDRRRSKYQLITTVRTNKASDDKCFSCVTQEEDANGNIGVTLSKDLMAVAGDALKTNITTLGPLVLPTSEQLLFFTTLVGKKLFKMKIKPYIPDFKLAFEHFCIHAGGRAVLDELEKNLQLSPWHMEPSRMTLYRFGNTSSSSLWYELAYTEAKGRIKKGDRTWQIAFGSGFKCNSAVWKALRTINPVKEKSPWIDEIDQFPVDVPKVSTI*