AlfalfaGEDB | Msa1294670

M. sativa cultivar zhongmu-4 / Msa1294670

KEGG: K18826
GO: -
PFAM: PF10294.12

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1294670	sp\|F4JNX3\|CMKMT_ARATH	63.776	196	69	1	6	201	108	301	1.86e-91	273

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1294670	G7LC21	96.954	197	6	0	6	202	121	317	2.94e-139	400

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa1294670	0.800764	1.236868e-48	-8.615850e-47
Msa0140570	Msa1294670	0.806926	6.486838e-50	-8.615850e-47
Msa0381550	Msa1294670	0.817056	4.019036e-52	-8.615850e-47
Msa0520070	Msa1294670	0.817345	3.461140e-52	-8.615850e-47
Msa0871270	Msa1294670	0.801788	7.633558e-49	-8.615850e-47
Msa0880140	Msa1294670	0.800175	1.630604e-48	-8.615850e-47
Msa0938220	Msa1294670	0.807008	6.234496e-50	-8.615850e-47
Msa1294670	Msa1324780	0.813472	2.515585e-51	-8.615850e-47
Msa1294670	Msa1411070	0.807191	5.701199e-50	-8.615850e-47
Msa1294670	Msa1417870	0.804571	2.026185e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1294670	MtrunA17_Chr8g0371751	96.954	197	6	0	6	202	121	317	5.67e-143	400
Msa1294670	MtrunA17_Chr7g0273091	31.724	145	87	4	7	140	151	294	6.20e-12	63.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1294670	AT4G35987.1	63.776	196	69	1	6	201	108	301	1.89e-92	273

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Find 59 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCATTGGACCAGTTCT+TGG	0.305606	8_3:+52449940	Msa1294670:CDS
AAAGTTATTGAGCTTGGTTC+TGG	0.303422	8_3:+52448022	Msa1294670:CDS
AAATGAATGTGAAGGGGATG+TGG	0.564880	8_3:+52450313	Msa1294670:three_prime_UTR
AAATTACATAAGCGTGCAAT+TGG	0.428200	8_3:-52450237	None:intergenic
AAATTCATGATGTCCTCTGC+AGG	0.543905	8_3:+52447785	None:intergenic
AACAGTCAACCAACAATGAA+AGG	0.573682	8_3:-52450154	None:intergenic
AAGCTAACTCTGGAGCATTT+GGG	0.339254	8_3:+52449208	Msa1294670:CDS
AAGGCACAAATGAATGTGAA+GGG	0.575283	8_3:+52450306	Msa1294670:three_prime_UTR
AAGTCTGGAAGCGTCATGAG+GGG	0.697903	8_3:+52450017	Msa1294670:CDS
AGAACTATGACAAAGAAGTC+TGG	0.486522	8_3:+52450002	Msa1294670:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTCACATAGAGATATATTC+AGG	+	chr8_3:52447846-52447865	Msa1294670:CDS	30.0%
CTTTGTTGAAATTTATACCC+AGG	-	chr8_3:52448265-52448284	None:intergenic	30.0%
ATGAGTTCAAATAAGGCTTA+GGG	-	chr8_3:52448460-52448479	None:intergenic	30.0%
AATGAGTTCAAATAAGGCTT+AGG	-	chr8_3:52448461-52448480	None:intergenic	30.0%
CTCACAAATGAGTTCAAATA+AGG	-	chr8_3:52448467-52448486	None:intergenic	30.0%
TATTTGAACTCATTTGTGAG+CGG	+	chr8_3:52448467-52448486	Msa1294670:intron	30.0%
GACATTAAATGACAAACACT+TGG	-	chr8_3:52448646-52448665	None:intergenic	30.0%
ATATCATTGTTGCAAGTGAT+TGG	+	chr8_3:52449301-52449320	Msa1294670:CDS	30.0%
GCATCTAAGTAAGAAATTAC+AGG	-	chr8_3:52449431-52449450	None:intergenic	30.0%
AAAGACCAAAATAAAAGGCA+AGG	-	chr8_3:52449770-52449789	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	52447794	52450534	52447794	ID=Msa1294670;Name=Msa1294670
chr8_3	mRNA	52447794	52450534	52447794	ID=Msa1294670-mRNA-1;Parent=Msa1294670;Name=Msa1294670-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|1\|0.75\|1\|1\|1\|4\|411\|202
chr8_3	exon	52447794	52447867	52447794	ID=Msa1294670-mRNA-1:exon:14908;Parent=Msa1294670-mRNA-1
chr8_3	exon	52448015	52448130	52448015	ID=Msa1294670-mRNA-1:exon:14909;Parent=Msa1294670-mRNA-1
chr8_3	exon	52449190	52449322	52449190	ID=Msa1294670-mRNA-1:exon:14910;Parent=Msa1294670-mRNA-1
chr8_3	exon	52449838	52450534	52449838	ID=Msa1294670-mRNA-1:exon:14911;Parent=Msa1294670-mRNA-1
chr8_3	CDS	52447794	52447867	52447794	ID=Msa1294670-mRNA-1:cds;Parent=Msa1294670-mRNA-1
chr8_3	CDS	52448015	52448130	52448015	ID=Msa1294670-mRNA-1:cds;Parent=Msa1294670-mRNA-1
chr8_3	CDS	52449190	52449322	52449190	ID=Msa1294670-mRNA-1:cds;Parent=Msa1294670-mRNA-1
chr8_3	CDS	52449838	52450123	52449838	ID=Msa1294670-mRNA-1:cds;Parent=Msa1294670-mRNA-1
chr8_3	three_prime_UTR	52450124	52450534	52450124	ID=Msa1294670-mRNA-1:three_prime_utr;Parent=Msa1294670-mRNA-1

Gene Sequence

>Msa1294670

ATGTCCTCTGCAGGTAATTGGCCATCAGAAGATGTTCTTGCTCATTATTGCTTGTCACATAGAGATATATTCAGGTCTAAAAAAGTTATTGAGCTTGGTTCTGGCTATGGCTTGGCTGGATTTGTTATTGCAGCTGTTACTGAGGCATCCGAGGTTGTAATCTCAGATGGAAATCCACAAGTAGTTGATTATACTCAGCGTAACATTGAAGCTAACTCTGGAGCATTTGGGGATACGGTTGTGAAGTCTATGAAGCTTCATTGGAATCAGGAAGATACTTCTAGTGTTGCAGATGCTTTTGATATCATTGTTGCAAGTGATTGCACTTTCTTCAAAGATTTCCACAGAGATCTTGCTCGAATTGTCAAGCACTTGTTATCAAAAATAGGATCTTCCGAGGCTATATTTTTAAGTCCAAAAAGAGGAAACTCATTGGACCAGTTCTTGGAGGTAGCTGAAGAACATGGCTTGCATTTCAGTGTAACAGAGAACTATGACAAAGAAGTCTGGAAGCGTCATGAGGGGTTCTTGAATGGTGCAGATAGGGACTATTGGCCCAGTTATGAGACAGGTCACTGCTATCCACTCTTAATCAGAATCACCCTATGA

Protein sequence

>Msa1294670

MSSAGNWPSEDVLAHYCLSHRDIFRSKKVIELGSGYGLAGFVIAAVTEASEVVISDGNPQVVDYTQRNIEANSGAFGDTVVKSMKLHWNQEDTSSVADAFDIIVASDCTFFKDFHRDLARIVKHLLSKIGSSEAIFLSPKRGNSLDQFLEVAEEHGLHFSVTENYDKEVWKRHEGFLNGADRDYWPSYETGHCYPLLIRITL*