AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1294670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1294670 MtrunA17_Chr8g0371751 96.954 197 6 0 6 202 121 317 5.67e-143 400
Msa1294670 MtrunA17_Chr7g0273091 31.724 145 87 4 7 140 151 294 6.20e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1294670 AT4G35987.1 63.776 196 69 1 6 201 108 301 1.89e-92 273

Find 59 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATTCATTTGTGCCTTCT+TGG 0.088397 8_3:-52450301 None:intergenic
CTGGGACCTCCTTTCATTGT+TGG 0.288697 8_3:+52450145 Msa1294670:three_prime_UTR
GAAGCTAACTCTGGAGCATT+TGG 0.293032 8_3:+52449207 Msa1294670:CDS
TGATGTCCTCTGCAGGTAAT+TGG 0.299763 8_3:+52447792 None:intergenic
AAAGTTATTGAGCTTGGTTC+TGG 0.303422 8_3:+52448022 Msa1294670:CDS
AAACTCATTGGACCAGTTCT+TGG 0.305606 8_3:+52449940 Msa1294670:CDS
AAGCTAACTCTGGAGCATTT+GGG 0.339254 8_3:+52449208 Msa1294670:CDS
GCAGTGACCTGTCTCATAAC+TGG 0.342868 8_3:-52450071 None:intergenic
TGTCACATAGAGATATATTC+AGG 0.349449 8_3:+52447846 Msa1294670:CDS
TCACTAGACATTTCTTCTGT+TGG 0.357231 8_3:-52450205 None:intergenic
ATTGAGCTTGGTTCTGGCTA+TGG 0.376358 8_3:+52448028 Msa1294670:CDS
GGTTCTGGCTATGGCTTGGC+TGG 0.380102 8_3:+52448037 Msa1294670:CDS
CTCTGGAGCATTTGGGGATA+CGG 0.404971 8_3:+52449215 Msa1294670:CDS
TGAAGTCTATGAAGCTTCAT+TGG 0.406708 8_3:+52449241 Msa1294670:CDS
GGTTCTTGAATGGTGCAGAT+AGG 0.421817 8_3:+52450038 Msa1294670:CDS
AAATTACATAAGCGTGCAAT+TGG 0.428200 8_3:-52450237 None:intergenic
CTTTGCAGTGAGATTATGTA+TGG 0.428638 8_3:+52450453 Msa1294670:three_prime_UTR
ATGTCTAGAAGAAAGTTCAT+AGG 0.431482 8_3:-52450117 None:intergenic
CTAGACATTTCTTCTGTTGG+AGG 0.437437 8_3:-52450202 None:intergenic
TATGAAGCTTCATTGGAATC+AGG 0.447712 8_3:+52449248 Msa1294670:CDS
ATATCATTGTTGCAAGTGAT+TGG 0.449239 8_3:+52449301 Msa1294670:CDS
TTTCTTCTAGACATTCTGCC+TGG 0.455165 8_3:+52450126 Msa1294670:three_prime_UTR
GCTTGGTTCTGGCTATGGCT+TGG 0.461601 8_3:+52448033 Msa1294670:CDS
CGTCATGAGGGGTTCTTGAA+TGG 0.471374 8_3:+52450028 Msa1294670:CDS
AGAACTATGACAAAGAAGTC+TGG 0.486522 8_3:+52450002 Msa1294670:CDS
CTCATTGGACCAGTTCTTGG+AGG 0.500619 8_3:+52449943 Msa1294670:CDS
CTTCAGCTACCTCCAAGAAC+TGG 0.501959 8_3:-52449952 None:intergenic
GTTCTTGAATGGTGCAGATA+GGG 0.505855 8_3:+52450039 Msa1294670:CDS
ATGGTGCAGATAGGGACTAT+TGG 0.521392 8_3:+52450047 Msa1294670:CDS
CGTAACATTGAAGCTAACTC+TGG 0.523854 8_3:+52449198 Msa1294670:CDS
TCCATCTGAGATTACAACCT+CGG 0.528432 8_3:-52448089 None:intergenic
GACTGTTCGTAAAAGATAAG+TGG 0.535083 8_3:+52450170 Msa1294670:three_prime_UTR
AAATTCATGATGTCCTCTGC+AGG 0.543905 8_3:+52447785 None:intergenic
AGGATACAATCAACTACTTG+TGG 0.545485 8_3:-52448115 None:intergenic
CAGTGACCTGTCTCATAACT+GGG 0.554773 8_3:-52450070 None:intergenic
TCCGAGGTTGTAATCTCAGA+TGG 0.555442 8_3:+52448088 Msa1294670:CDS
AGCTAACTCTGGAGCATTTG+GGG 0.555559 8_3:+52449209 Msa1294670:CDS
AGATTATGTATGGAAAGACT+TGG 0.557548 8_3:+52450463 Msa1294670:three_prime_UTR
TGTCTAGAAGAAAGTTCATA+GGG 0.559803 8_3:-52450116 None:intergenic
TTCTTCTAGACATTCTGCCT+GGG 0.561415 8_3:+52450127 Msa1294670:three_prime_UTR
AAATGAATGTGAAGGGGATG+TGG 0.564880 8_3:+52450313 Msa1294670:three_prime_UTR
TGTTATTGCAGCTGTTACTG+AGG 0.565121 8_3:+52448063 Msa1294670:CDS
TATTGGCCCAGTTATGAGAC+AGG 0.568174 8_3:+52450064 Msa1294670:CDS
GATTATGTATGGAAAGACTT+GGG 0.572964 8_3:+52450464 Msa1294670:three_prime_UTR
AACAGTCAACCAACAATGAA+AGG 0.573682 8_3:-52450154 None:intergenic
AAGGCACAAATGAATGTGAA+GGG 0.575283 8_3:+52450306 Msa1294670:three_prime_UTR
GAAGGCACAAATGAATGTGA+AGG 0.581228 8_3:+52450305 Msa1294670:three_prime_UTR
GAAGTCTGGAAGCGTCATGA+GGG 0.584126 8_3:+52450016 Msa1294670:CDS
CAACAATGAAAGGAGGTCCC+AGG 0.585920 8_3:-52450144 None:intergenic
AGCTGTTACTGAGGCATCCG+AGG 0.587060 8_3:+52448072 Msa1294670:CDS
AGAAGTCTGGAAGCGTCATG+AGG 0.596564 8_3:+52450015 Msa1294670:CDS
TTGGAGGTAGCTGAAGAACA+TGG 0.609656 8_3:+52449959 Msa1294670:CDS
TGAGCAAGAACATCTTCTGA+TGG 0.613117 8_3:-52447815 None:intergenic
AGGGTGATTCTGATTAAGAG+TGG 0.626994 8_3:-52450097 None:intergenic
TGATGGCCAATTACCTGCAG+AGG 0.627373 8_3:-52447798 None:intergenic
AGGCACAAATGAATGTGAAG+GGG 0.673008 8_3:+52450307 Msa1294670:three_prime_UTR
CAATTCGAGCAAGATCTCTG+TGG 0.688833 8_3:-52449856 None:intergenic
AAGTCTGGAAGCGTCATGAG+GGG 0.697903 8_3:+52450017 Msa1294670:CDS
AGTCAACCAACAATGAAAGG+AGG 0.701573 8_3:-52450151 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATCATTAAAAATACAAA+GGG - chr8_3:52448299-52448318 None:intergenic 10.0%
!! TAAATATATTGTTTATTTCA+TGG + chr8_3:52449528-52449547 Msa1294670:intron 10.0%
!!! TCTATATTTTTTCTTTTATG+AGG + chr8_3:52447877-52447896 Msa1294670:intron 15.0%
!! GTTTATCATTAAAAATACAA+AGG - chr8_3:52448300-52448319 None:intergenic 15.0%
!! CCTAATTATACAATTATATA+TGG - chr8_3:52448839-52448858 None:intergenic 15.0%
!! CCATATATAATTGTATAATT+AGG + chr8_3:52448836-52448855 Msa1294670:intron 15.0%
!!! TGATTTTTAAAAAACATACA+TGG + chr8_3:52448881-52448900 Msa1294670:intron 15.0%
!! CTATTTATAGAAAATGTTAT+TGG - chr8_3:52449453-52449472 None:intergenic 15.0%
!! AAATGATAAAAAGTTAACAA+AGG - chr8_3:52449634-52449653 None:intergenic 15.0%
!!! TCATTTTCAATATTTAAAGT+TGG + chr8_3:52449648-52449667 Msa1294670:intron 15.0%
!!! AATAAAGTTTTAAAAATCTC+AGG - chr8_3:52450384-52450403 None:intergenic 15.0%
!! TGTTAACTAATGAGTAATAA+TGG - chr8_3:52448430-52448449 None:intergenic 20.0%
!! ATTTCCTACTTTAGATAATA+TGG + chr8_3:52448610-52448629 Msa1294670:intron 20.0%
!! TTGTATTTAGTATTTACTTG+TGG + chr8_3:52448683-52448702 Msa1294670:intron 20.0%
!! ATTAGTTATTACAAGCAAAT+TGG + chr8_3:52448733-52448752 Msa1294670:intron 20.0%
!!! TTTTAAAAAACATACATGGT+TGG + chr8_3:52448885-52448904 Msa1294670:intron 20.0%
!!! TTAAATATTTTCATGTCAAG+TGG + chr8_3:52449145-52449164 Msa1294670:intron 20.0%
!! TTGATAAATATAAGAGTCAA+AGG + chr8_3:52449475-52449494 Msa1294670:intron 20.0%
!! TGATAAATATAAGAGTCAAA+GGG + chr8_3:52449476-52449495 Msa1294670:intron 20.0%
!! AAACAATATATTTAATGCTC+CGG - chr8_3:52449522-52449541 None:intergenic 20.0%
!! ATATATTGTTTATTTCATGG+AGG + chr8_3:52449531-52449550 Msa1294670:intron 20.0%
!!! AAATTTTTGCATTCATGTTA+TGG + chr8_3:52449579-52449598 Msa1294670:intron 20.0%
!!! AATGACTAAATATTTTCAAG+TGG + chr8_3:52449683-52449702 Msa1294670:intron 20.0%
!! ATCATAAAGACCAAAATAAA+AGG - chr8_3:52449775-52449794 None:intergenic 20.0%
!! CTTTATATTATGACATTGAA+TGG + chr8_3:52449803-52449822 Msa1294670:intron 20.0%
!!! ATATTTTTAAGTCCAAAAAG+AGG + chr8_3:52449917-52449936 Msa1294670:CDS 20.0%
! AAAATGATGCTAATATACAG+AGG - chr8_3:52447950-52447969 None:intergenic 25.0%
! TCTAAAAAAGTTATTGAGCT+TGG + chr8_3:52448016-52448035 Msa1294670:CDS 25.0%
! TCATCAATAACTATAGCAAA+AGG - chr8_3:52448138-52448157 None:intergenic 25.0%
! TTCAATACTTGTTCTACAAA+TGG + chr8_3:52448214-52448233 Msa1294670:intron 25.0%
! AATGATAAACATGAAACAAC+TGG + chr8_3:52448310-52448329 Msa1294670:intron 25.0%
! ATTGTGAATAAAGGTCTAAA+AGG - chr8_3:52448354-52448373 None:intergenic 25.0%
!! TTTTAGACCTTTATTCACAA+TGG + chr8_3:52448353-52448372 Msa1294670:intron 25.0%
!! ACTTCCATATTATCTAAAGT+AGG - chr8_3:52448617-52448636 None:intergenic 25.0%
! ACATTAAATGACAAACACTT+GGG - chr8_3:52448645-52448664 None:intergenic 25.0%
! TTTAGTATTTACTTGTGGAA+AGG + chr8_3:52448688-52448707 Msa1294670:intron 25.0%
!!! AACTAATTCAAGTTTTTGAG+TGG - chr8_3:52448720-52448739 None:intergenic 25.0%
! CAATTATATATGGATAGTCA+AGG - chr8_3:52448829-52448848 None:intergenic 25.0%
! AATGCTGTGTTTATTACATT+GGG - chr8_3:52449060-52449079 None:intergenic 25.0%
! TAATGCTGTGTTTATTACAT+TGG - chr8_3:52449061-52449080 None:intergenic 25.0%
!! TTTTCTTTCAAAAGTTTGCA+TGG + chr8_3:52449093-52449112 Msa1294670:intron 25.0%
! TAATGAAGCATATTCTCAAA+AGG - chr8_3:52449128-52449147 None:intergenic 25.0%
! AATAAGATTGTCATCTAAGT+TGG - chr8_3:52449382-52449401 None:intergenic 25.0%
!!! AATATTTTCAAGTGGAGAAA+AGG + chr8_3:52449691-52449710 Msa1294670:intron 25.0%
!!! AAGCACTTGTTATCAAAAAT+AGG + chr8_3:52449881-52449900 Msa1294670:CDS 25.0%
!!! ACGCTTATGTAATTTTTTGT+TGG + chr8_3:52450245-52450264 Msa1294670:three_prime_UTR 25.0%
!!! CGCTTATGTAATTTTTTGTT+GGG + chr8_3:52450246-52450265 Msa1294670:three_prime_UTR 25.0%
!!! GCTTATGTAATTTTTTGTTG+GGG + chr8_3:52450247-52450266 Msa1294670:three_prime_UTR 25.0%
TGTCACATAGAGATATATTC+AGG + chr8_3:52447846-52447865 Msa1294670:CDS 30.0%
! TAGAGATATATTCAGGTTTG+AGG + chr8_3:52447853-52447872 Msa1294670:intron 30.0%
! AGACTGGTATGATTTCTTTT+AGG + chr8_3:52447993-52448012 Msa1294670:intron 30.0%
CTTTGTTGAAATTTATACCC+AGG - chr8_3:52448265-52448284 None:intergenic 30.0%
ATGAGTTCAAATAAGGCTTA+GGG - chr8_3:52448460-52448479 None:intergenic 30.0%
AATGAGTTCAAATAAGGCTT+AGG - chr8_3:52448461-52448480 None:intergenic 30.0%
CTCACAAATGAGTTCAAATA+AGG - chr8_3:52448467-52448486 None:intergenic 30.0%
TATTTGAACTCATTTGTGAG+CGG + chr8_3:52448467-52448486 Msa1294670:intron 30.0%
GACATTAAATGACAAACACT+TGG - chr8_3:52448646-52448665 None:intergenic 30.0%
ATATCATTGTTGCAAGTGAT+TGG + chr8_3:52449301-52449320 Msa1294670:CDS 30.0%
GCATCTAAGTAAGAAATTAC+AGG - chr8_3:52449431-52449450 None:intergenic 30.0%
! TTTTGCATTCATGTTATGGT+TGG + chr8_3:52449583-52449602 Msa1294670:intron 30.0%
AAAGACCAAAATAAAAGGCA+AGG - chr8_3:52449770-52449789 None:intergenic 30.0%
TGGACTTAAAAATATAGCCT+CGG - chr8_3:52449912-52449931 None:intergenic 30.0%
TGTCTAGAAGAAAGTTCATA+GGG - chr8_3:52450119-52450138 None:intergenic 30.0%
ATGTCTAGAAGAAAGTTCAT+AGG - chr8_3:52450120-52450139 None:intergenic 30.0%
AAATTACATAAGCGTGCAAT+TGG - chr8_3:52450240-52450259 None:intergenic 30.0%
AGATTATGTATGGAAAGACT+TGG + chr8_3:52450463-52450482 Msa1294670:three_prime_UTR 30.0%
GATTATGTATGGAAAGACTT+GGG + chr8_3:52450464-52450483 Msa1294670:three_prime_UTR 30.0%
AAAGAAATCATACCAGTCTG+AGG - chr8_3:52447992-52448011 None:intergenic 35.0%
AAAGTTATTGAGCTTGGTTC+TGG + chr8_3:52448022-52448041 Msa1294670:CDS 35.0%
AGGATACAATCAACTACTTG+TGG - chr8_3:52448118-52448137 None:intergenic 35.0%
TTGTTCTACAAATGGATGTG+AGG + chr8_3:52448222-52448241 Msa1294670:intron 35.0%
CTAGCATCCATTGTGAATAA+AGG - chr8_3:52448363-52448382 None:intergenic 35.0%
CTTTATTCACAATGGATGCT+AGG + chr8_3:52448361-52448380 Msa1294670:intron 35.0%
TCTTGTCATATGTATGCATG+TGG + chr8_3:52448766-52448785 Msa1294670:intron 35.0%
CTTGTCATATGTATGCATGT+GGG + chr8_3:52448767-52448786 Msa1294670:intron 35.0%
AAACAAATGTACTCTACTGC+TGG + chr8_3:52448957-52448976 Msa1294670:intron 35.0%
AAGGGAGAAATGTCAAACAT+GGG - chr8_3:52449018-52449037 None:intergenic 35.0%
AAAGGGAGAAATGTCAAACA+TGG - chr8_3:52449019-52449038 None:intergenic 35.0%
GTTTGACATTTCTCCCTTTA+CGG + chr8_3:52449020-52449039 Msa1294670:intron 35.0%
TTTGACATTTCTCCCTTTAC+GGG + chr8_3:52449021-52449040 Msa1294670:intron 35.0%
TGAAGTCTATGAAGCTTCAT+TGG + chr8_3:52449241-52449260 Msa1294670:CDS 35.0%
TATGAAGCTTCATTGGAATC+AGG + chr8_3:52449248-52449267 Msa1294670:CDS 35.0%
! TTCATGTTATGGTTGGTCAA+TGG + chr8_3:52449590-52449609 Msa1294670:intron 35.0%
ATCAAAAATAGGATCTTCCG+AGG + chr8_3:52449892-52449911 Msa1294670:CDS 35.0%
TCCAAAAAGAGGAAACTCAT+TGG + chr8_3:52449928-52449947 Msa1294670:CDS 35.0%
! TCCAATGAGTTTCCTCTTTT+TGG - chr8_3:52449932-52449951 None:intergenic 35.0%
AGAACTATGACAAAGAAGTC+TGG + chr8_3:52450002-52450021 Msa1294670:CDS 35.0%
AACAGTCAACCAACAATGAA+AGG - chr8_3:52450157-52450176 None:intergenic 35.0%
GACTGTTCGTAAAAGATAAG+TGG + chr8_3:52450170-52450189 Msa1294670:three_prime_UTR 35.0%
TCACTAGACATTTCTTCTGT+TGG - chr8_3:52450208-52450227 None:intergenic 35.0%
! AGTACTTTTGTCATCCAAGA+AGG + chr8_3:52450287-52450306 Msa1294670:three_prime_UTR 35.0%
AAGGCACAAATGAATGTGAA+GGG + chr8_3:52450306-52450325 Msa1294670:three_prime_UTR 35.0%
CTTTGCAGTGAGATTATGTA+TGG + chr8_3:52450453-52450472 Msa1294670:three_prime_UTR 35.0%
TGAGCAAGAACATCTTCTGA+TGG - chr8_3:52447818-52447837 None:intergenic 40.0%
! ATTTTTCTGTGACCTCAGAC+TGG + chr8_3:52447977-52447996 Msa1294670:intron 40.0%
TGTTATTGCAGCTGTTACTG+AGG + chr8_3:52448063-52448082 Msa1294670:CDS 40.0%
! TCCATCTGAGATTACAACCT+CGG - chr8_3:52448092-52448111 None:intergenic 40.0%
TCTACAAATGGATGTGAGGA+AGG + chr8_3:52448226-52448245 Msa1294670:intron 40.0%
CTACAAATGGATGTGAGGAA+GGG + chr8_3:52448227-52448246 Msa1294670:intron 40.0%
AAGGGAAAATAGTGTCTCCT+GGG + chr8_3:52448245-52448264 Msa1294670:intron 40.0%
ATGACCTTATCAGACGAGTT+TGG + chr8_3:52448549-52448568 Msa1294670:intron 40.0%
TGGATAGTCAAGGTTAGACT+TGG - chr8_3:52448819-52448838 None:intergenic 40.0%
! ACATGGGCGAAGTATTTCAA+CGG - chr8_3:52449002-52449021 None:intergenic 40.0%
TTGACATTTCTCCCTTTACG+GGG + chr8_3:52449022-52449041 Msa1294670:intron 40.0%
CGTAACATTGAAGCTAACTC+TGG + chr8_3:52449198-52449217 Msa1294670:CDS 40.0%
!! AAGCTAACTCTGGAGCATTT+GGG + chr8_3:52449208-52449227 Msa1294670:CDS 40.0%
TCAGCATGTGGTGATAATTC+CGG + chr8_3:52449500-52449519 Msa1294670:intron 40.0%
!! GCTTGCCTTGCCTTTTATTT+TGG + chr8_3:52449762-52449781 Msa1294670:intron 40.0%
AAACTCATTGGACCAGTTCT+TGG + chr8_3:52449940-52449959 Msa1294670:CDS 40.0%
GTTCTTGAATGGTGCAGATA+GGG + chr8_3:52450039-52450058 Msa1294670:CDS 40.0%
AGGGTGATTCTGATTAAGAG+TGG - chr8_3:52450100-52450119 None:intergenic 40.0%
TTTCTTCTAGACATTCTGCC+TGG + chr8_3:52450126-52450145 Msa1294670:three_prime_UTR 40.0%
TTCTTCTAGACATTCTGCCT+GGG + chr8_3:52450127-52450146 Msa1294670:three_prime_UTR 40.0%
AGTCAACCAACAATGAAAGG+AGG - chr8_3:52450154-52450173 None:intergenic 40.0%
CTAGACATTTCTTCTGTTGG+AGG - chr8_3:52450205-52450224 None:intergenic 40.0%
CACATTCATTTGTGCCTTCT+TGG - chr8_3:52450304-52450323 None:intergenic 40.0%
! GAAGGCACAAATGAATGTGA+AGG + chr8_3:52450305-52450324 Msa1294670:three_prime_UTR 40.0%
AGGCACAAATGAATGTGAAG+GGG + chr8_3:52450307-52450326 Msa1294670:three_prime_UTR 40.0%
AAATGAATGTGAAGGGGATG+TGG + chr8_3:52450313-52450332 Msa1294670:three_prime_UTR 40.0%
!! ATTGAGCTTGGTTCTGGCTA+TGG + chr8_3:52448028-52448047 Msa1294670:CDS 45.0%
TCCGAGGTTGTAATCTCAGA+TGG + chr8_3:52448088-52448107 Msa1294670:CDS 45.0%
GAAGGGAAAATAGTGTCTCC+TGG + chr8_3:52448244-52448263 Msa1294670:intron 45.0%
CGTGATTTCATGCTGTTAGC+AGG + chr8_3:52448503-52448522 Msa1294670:intron 45.0%
ACCTTATCAGACGAGTTTGG+TGG + chr8_3:52448552-52448571 Msa1294670:intron 45.0%
!! AGACGAGTTTGGTGGCATTT+TGG + chr8_3:52448560-52448579 Msa1294670:intron 45.0%
! GGGTGTGCTGATAGACAAAT+TGG + chr8_3:52448787-52448806 Msa1294670:intron 45.0%
TGACATTTCTCCCTTTACGG+GGG + chr8_3:52449023-52449042 Msa1294670:intron 45.0%
!! GAAGCTAACTCTGGAGCATT+TGG + chr8_3:52449207-52449226 Msa1294670:CDS 45.0%
!! AGCTAACTCTGGAGCATTTG+GGG + chr8_3:52449209-52449228 Msa1294670:CDS 45.0%
CAATTCGAGCAAGATCTCTG+TGG - chr8_3:52449859-52449878 None:intergenic 45.0%
TTGGAGGTAGCTGAAGAACA+TGG + chr8_3:52449959-52449978 Msa1294670:CDS 45.0%
GGTTCTTGAATGGTGCAGAT+AGG + chr8_3:52450038-52450057 Msa1294670:CDS 45.0%
ATGGTGCAGATAGGGACTAT+TGG + chr8_3:52450047-52450066 Msa1294670:CDS 45.0%
TATTGGCCCAGTTATGAGAC+AGG + chr8_3:52450064-52450083 Msa1294670:CDS 45.0%
CAGTGACCTGTCTCATAACT+GGG - chr8_3:52450073-52450092 None:intergenic 45.0%
!! TTTAAAAATCATATTATATA+GGG - chr8_3:52448872-52448891 None:intergenic 5.0%
!!! TTTTAAAAATCATATTATAT+AGG - chr8_3:52448873-52448892 None:intergenic 5.0%
!!! TTTGTTTTTAATATAAATAT+AGG - chr8_3:52448944-52448963 None:intergenic 5.0%
TGATGGCCAATTACCTGCAG+AGG - chr8_3:52447801-52447820 None:intergenic 50.0%
GCCACCAAACTCGTCTGATA+AGG - chr8_3:52448556-52448575 None:intergenic 50.0%
CGAGAAATCTCCCCCGTAAA+GGG - chr8_3:52449036-52449055 None:intergenic 50.0%
ACGAGAAATCTCCCCCGTAA+AGG - chr8_3:52449037-52449056 None:intergenic 50.0%
!! CTCTGGAGCATTTGGGGATA+CGG + chr8_3:52449215-52449234 Msa1294670:CDS 50.0%
GAGTCAAAGGGTTCAGCATG+TGG + chr8_3:52449488-52449507 Msa1294670:intron 50.0%
GCTGCATGCATGTGAATCTG+CGG - chr8_3:52449744-52449763 None:intergenic 50.0%
CTCATTGGACCAGTTCTTGG+AGG + chr8_3:52449943-52449962 Msa1294670:CDS 50.0%
CTTCAGCTACCTCCAAGAAC+TGG - chr8_3:52449955-52449974 None:intergenic 50.0%
!! AGAAGTCTGGAAGCGTCATG+AGG + chr8_3:52450015-52450034 Msa1294670:CDS 50.0%
!! GAAGTCTGGAAGCGTCATGA+GGG + chr8_3:52450016-52450035 Msa1294670:CDS 50.0%
!! AAGTCTGGAAGCGTCATGAG+GGG + chr8_3:52450017-52450036 Msa1294670:CDS 50.0%
CGTCATGAGGGGTTCTTGAA+TGG + chr8_3:52450028-52450047 Msa1294670:CDS 50.0%
GCAGTGACCTGTCTCATAAC+TGG - chr8_3:52450074-52450093 None:intergenic 50.0%
CAACAATGAAAGGAGGTCCC+AGG - chr8_3:52450147-52450166 None:intergenic 50.0%
CTGGGACCTCCTTTCATTGT+TGG + chr8_3:52450145-52450164 Msa1294670:three_prime_UTR 50.0%
!! GCTTGGTTCTGGCTATGGCT+TGG + chr8_3:52448033-52448052 Msa1294670:CDS 55.0%
! AGCTGTTACTGAGGCATCCG+AGG + chr8_3:52448072-52448091 Msa1294670:CDS 55.0%
! GGTTCTGGCTATGGCTTGGC+TGG + chr8_3:52448037-52448056 Msa1294670:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 52447794 52450534 52447794 ID=Msa1294670;Name=Msa1294670
chr8_3 mRNA 52447794 52450534 52447794 ID=Msa1294670-mRNA-1;Parent=Msa1294670;Name=Msa1294670-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|1|0.75|1|1|1|4|411|202
chr8_3 exon 52447794 52447867 52447794 ID=Msa1294670-mRNA-1:exon:14908;Parent=Msa1294670-mRNA-1
chr8_3 exon 52448015 52448130 52448015 ID=Msa1294670-mRNA-1:exon:14909;Parent=Msa1294670-mRNA-1
chr8_3 exon 52449190 52449322 52449190 ID=Msa1294670-mRNA-1:exon:14910;Parent=Msa1294670-mRNA-1
chr8_3 exon 52449838 52450534 52449838 ID=Msa1294670-mRNA-1:exon:14911;Parent=Msa1294670-mRNA-1
chr8_3 CDS 52447794 52447867 52447794 ID=Msa1294670-mRNA-1:cds;Parent=Msa1294670-mRNA-1
chr8_3 CDS 52448015 52448130 52448015 ID=Msa1294670-mRNA-1:cds;Parent=Msa1294670-mRNA-1
chr8_3 CDS 52449190 52449322 52449190 ID=Msa1294670-mRNA-1:cds;Parent=Msa1294670-mRNA-1
chr8_3 CDS 52449838 52450123 52449838 ID=Msa1294670-mRNA-1:cds;Parent=Msa1294670-mRNA-1
chr8_3 three_prime_UTR 52450124 52450534 52450124 ID=Msa1294670-mRNA-1:three_prime_utr;Parent=Msa1294670-mRNA-1
Gene Sequence

>Msa1294670

ATGTCCTCTGCAGGTAATTGGCCATCAGAAGATGTTCTTGCTCATTATTGCTTGTCACATAGAGATATATTCAGGTCTAAAAAAGTTATTGAGCTTGGTTCTGGCTATGGCTTGGCTGGATTTGTTATTGCAGCTGTTACTGAGGCATCCGAGGTTGTAATCTCAGATGGAAATCCACAAGTAGTTGATTATACTCAGCGTAACATTGAAGCTAACTCTGGAGCATTTGGGGATACGGTTGTGAAGTCTATGAAGCTTCATTGGAATCAGGAAGATACTTCTAGTGTTGCAGATGCTTTTGATATCATTGTTGCAAGTGATTGCACTTTCTTCAAAGATTTCCACAGAGATCTTGCTCGAATTGTCAAGCACTTGTTATCAAAAATAGGATCTTCCGAGGCTATATTTTTAAGTCCAAAAAGAGGAAACTCATTGGACCAGTTCTTGGAGGTAGCTGAAGAACATGGCTTGCATTTCAGTGTAACAGAGAACTATGACAAAGAAGTCTGGAAGCGTCATGAGGGGTTCTTGAATGGTGCAGATAGGGACTATTGGCCCAGTTATGAGACAGGTCACTGCTATCCACTCTTAATCAGAATCACCCTATGA
Protein sequence

>Msa1294670

MSSAGNWPSEDVLAHYCLSHRDIFRSKKVIELGSGYGLAGFVIAAVTEASEVVISDGNPQVVDYTQRNIEANSGAFGDTVVKSMKLHWNQEDTSSVADAFDIIVASDCTFFKDFHRDLARIVKHLLSKIGSSEAIFLSPKRGNSLDQFLEVAEEHGLHFSVTENYDKEVWKRHEGFLNGADRDYWPSYETGHCYPLLIRITL*