AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1345070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1345070 MtrunA17_Chr4g0027321 55.172 174 61 6 61 228 130 292 2.02e-49 163
Msa1345070 MtrunA17_Chr3g0119711 40.191 209 99 10 33 228 3 198 1.47e-29 109
Msa1345070 MtrunA17_Chr3g0120641 36.199 221 116 8 28 228 26 241 2.73e-20 86.3
Msa1345070 MtrunA17_Chr3g0120941 36.139 202 103 10 40 228 54 242 9.55e-20 84.7
Msa1345070 MtrunA17_Chr3g0120951 37.278 169 84 9 69 228 51 206 9.99e-19 81.3
Msa1345070 MtrunA17_Chr3g0120981 36.667 210 99 12 28 228 21 205 3.23e-17 77.0
Msa1345070 MtrunA17_Chr3g0120591 36.025 161 95 4 73 228 65 222 6.14e-17 76.6
Msa1345070 MtrunA17_Chr3g0120961 35.897 156 73 7 75 222 47 183 1.30e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 67 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTAATGTTTATGTATAAT+TGG 0.229634 tig0007057:+63623 Msa1345070:three_prime_UTR
GCTTTAAAAGTAATGTAATA+TGG 0.262097 tig0007057:-63367 None:intergenic
GATTTCAGCCGTTTCGATTT+CGG 0.272727 tig0007057:-62659 None:intergenic
TGGTTCTTTATATACGAATA+AGG 0.301977 tig0007057:+62904 Msa1345070:CDS
GAATCTTCTGGATCCGGTTT+CGG 0.312704 tig0007057:-62803 None:intergenic
GGTTATGGTTATGCTTGTTT+TGG 0.320799 tig0007057:+63515 Msa1345070:three_prime_UTR
TCACGACGCTTATACCAAAA+TGG 0.333746 tig0007057:-62881 None:intergenic
TCACTATACTCAGAATCTTC+TGG 0.339930 tig0007057:-62815 None:intergenic
GAAATCGAAACGGCTGAAAT+CGG 0.340115 tig0007057:+62661 Msa1345070:CDS
AATTAATTAAATAAATATGA+TGG 0.373275 tig0007057:+63566 Msa1345070:three_prime_UTR
AATACACATCGCCATGGTTA+TGG 0.379705 tig0007057:+63500 Msa1345070:exon
GTGTTTCAAAATGTCAAAAT+CGG 0.401236 tig0007057:+62628 Msa1345070:exon
ATGAAACACTCTTTGACAAC+AGG 0.409120 tig0007057:-63466 None:intergenic
GCTGAAATCGGAACCGAAAC+CGG 0.414648 tig0007057:+62790 Msa1345070:CDS
ATCTGTGGAGGAGGAGGAGG+AGG 0.415650 tig0007057:+62757 Msa1345070:CDS
AAATCGAAACGGCTGAAATC+GGG 0.418006 tig0007057:+62662 Msa1345070:CDS
CTACTTCTTTATATGCTCCT+AGG 0.435411 tig0007057:+63344 Msa1345070:CDS
CAAAATGTCAAAATCGGAAC+AGG 0.438148 tig0007057:+62634 Msa1345070:exon
TACTCAGAATCTTCTGGATC+CGG 0.439841 tig0007057:-62809 None:intergenic
TGCAGAGGGGTTTGAGAGTA+CGG 0.440533 tig0007057:-63144 None:intergenic
ACTTCATCAAACACAAAAGT+TGG 0.450228 tig0007057:-63298 None:intergenic
GGAGGAGAAACGGCTGAAAT+CGG 0.450703 tig0007057:+62778 Msa1345070:CDS
AGAAAGAAATGAAACTCTAA+AGG 0.454475 tig0007057:-63691 None:intergenic
AGAAAGAAATGAAACTCTAA+AGG 0.454475 tig0007057:-63785 None:intergenic
TTCATCTGTGGAGGAGGAGG+AGG 0.455235 tig0007057:+62754 Msa1345070:CDS
TACGCTGCAATACATGAGTC+TGG 0.467899 tig0007057:-63432 None:intergenic
AAAGTAATGTAATATGGCCT+AGG 0.487724 tig0007057:-63361 None:intergenic
CAATCAAACAAATATTAAGC+AGG 0.490074 tig0007057:+63186 Msa1345070:CDS
TAAAGCAAAAGCAGAAGCAA+AGG 0.500294 tig0007057:+63384 Msa1345070:CDS
ATTAATTAAATAAATATGAT+GGG 0.503492 tig0007057:+63567 Msa1345070:three_prime_UTR
ACACGATGAAGATTCATATG+AGG 0.514467 tig0007057:+62697 Msa1345070:CDS
CTTTATATACGAATAAGGCT+TGG 0.515687 tig0007057:+62909 Msa1345070:CDS
CACTGTCCACCACGTTGTGC+CGG 0.517844 tig0007057:+63085 Msa1345070:CDS
AGATTCATCTGTGGAGGAGG+AGG 0.522683 tig0007057:+62751 Msa1345070:CDS
GTCTGGAAAGTTGTTAGAGC+AGG 0.523457 tig0007057:-63415 None:intergenic
TATATACGAATAAGGCTTGG+AGG 0.536985 tig0007057:+62912 Msa1345070:CDS
AAAGCAAAAGCAGAAGCAAA+GGG 0.538089 tig0007057:+63385 Msa1345070:CDS
ACAAGATTCATCTGTGGAGG+AGG 0.546974 tig0007057:+62748 Msa1345070:CDS
TGAAGATTCATATGAGGATG+AGG 0.548643 tig0007057:+62703 Msa1345070:CDS
CACATCATCGCTTAAGTCGA+GGG 0.551344 tig0007057:-63119 None:intergenic
AACAGGAACCGAAATCGAAA+CGG 0.558710 tig0007057:+62651 Msa1345070:CDS
CTTCAAAGCAGCATTGCAGA+GGG 0.559702 tig0007057:-63158 None:intergenic
GCATATAAAGAAGTAGCACC+AGG 0.567371 tig0007057:-63337 None:intergenic
CATTAGTGACATAAATAACA+TGG 0.577507 tig0007057:-63608 None:intergenic
GTTGTCAAGTGTACCCATCC+TGG 0.578943 tig0007057:+63319 Msa1345070:CDS
ATATACGAATAAGGCTTGGA+GGG 0.581232 tig0007057:+62913 Msa1345070:CDS
TAAAGAAGTAGCACCAGGAT+GGG 0.590468 tig0007057:-63332 None:intergenic
GCACATCATCGCTTAAGTCG+AGG 0.599300 tig0007057:-63120 None:intergenic
TGTCCACCACGTTGTGCCGG+CGG 0.605052 tig0007057:+63088 Msa1345070:CDS
ATAAAGAAGTAGCACCAGGA+TGG 0.610411 tig0007057:-63333 None:intergenic
GAAACAAGATTCATCTGTGG+AGG 0.613961 tig0007057:+62745 Msa1345070:CDS
CGGCACAACGTGGTGGACAG+TGG 0.618592 tig0007057:-63084 None:intergenic
AAATCTAATACACATCGCCA+TGG 0.628062 tig0007057:+63494 Msa1345070:exon
TCTTCAAAGCAGCATTGCAG+AGG 0.630589 tig0007057:-63159 None:intergenic
AGATTCATATGAGGATGAGG+AGG 0.633765 tig0007057:+62706 Msa1345070:CDS
ATACCGCCGGCACAACGTGG+TGG 0.633986 tig0007057:-63091 None:intergenic
GAAACGGCTGAAATCGGGAT+CGG 0.652067 tig0007057:+62667 Msa1345070:CDS
AGAAGATTCTGAGTATAGTG+AGG 0.652619 tig0007057:+62817 Msa1345070:CDS
GTCGAGGGGTACGATACCGC+CGG 0.657761 tig0007057:-63104 None:intergenic
AACAAGCATAACCATAACCA+TGG 0.658373 tig0007057:-63511 None:intergenic
GCAAAAGCAGAAGCAAAGGG+AGG 0.667232 tig0007057:+63388 Msa1345070:CDS
ACTACACGTAAGTCTTGCCA+AGG 0.669722 tig0007057:-63542 None:intergenic
TGAGTATAGTGAGGAAGACG+AGG 0.671624 tig0007057:+62826 Msa1345070:CDS
CATGAAACAAGATTCATCTG+TGG 0.677002 tig0007057:+62742 Msa1345070:CDS
ACATCATCGCTTAAGTCGAG+GGG 0.690101 tig0007057:-63118 None:intergenic
ACGATACCGCCGGCACAACG+TGG 0.694116 tig0007057:-63094 None:intergenic
TTCAAAGCAGCATTGCAGAG+GGG 0.703047 tig0007057:-63157 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATGTTTATGTATAAT+TGG + tig0007057:63717-63736 Msa1345070:three_prime_UTR 10.0%
!! AAAAAATGTTTATGTATAAT+TGG + tig0007057:63717-63736 Msa1345070:three_prime_UTR 10.0%
!!! GCTTTAAAAGTAATGTAATA+TGG - tig0007057:63370-63389 None:intergenic 20.0%
!! CACTAATGTTTATGTATAAT+TGG + tig0007057:63623-63642 Msa1345070:three_prime_UTR 20.0%
!!! GCTTTAAAAGTAATGTAATA+TGG - tig0007057:63370-63389 None:intergenic 20.0%
!! CACTAATGTTTATGTATAAT+TGG + tig0007057:63623-63642 Msa1345070:three_prime_UTR 20.0%
!!! GATTTTGACATTTTGAAACA+CGG - tig0007057:62629-62648 None:intergenic 25.0%
! GTGTTTCAAAATGTCAAAAT+CGG + tig0007057:62628-62647 Msa1345070:exon 25.0%
! TGGTTCTTTATATACGAATA+AGG + tig0007057:62904-62923 Msa1345070:CDS 25.0%
!!! CACTTAATTTTGATTGATTC+TGG + tig0007057:63033-63052 Msa1345070:intron 25.0%
! CAATCAAACAAATATTAAGC+AGG + tig0007057:63186-63205 Msa1345070:CDS 25.0%
! CATTAGTGACATAAATAACA+TGG - tig0007057:63611-63630 None:intergenic 25.0%
! AGAAAGAAATGAAACTCTAA+AGG - tig0007057:63694-63713 None:intergenic 25.0%
!!! GATTTTGACATTTTGAAACA+CGG - tig0007057:62629-62648 None:intergenic 25.0%
! GTGTTTCAAAATGTCAAAAT+CGG + tig0007057:62628-62647 Msa1345070:exon 25.0%
! TGGTTCTTTATATACGAATA+AGG + tig0007057:62904-62923 Msa1345070:CDS 25.0%
!!! CACTTAATTTTGATTGATTC+TGG + tig0007057:63033-63052 Msa1345070:intron 25.0%
! CAATCAAACAAATATTAAGC+AGG + tig0007057:63186-63205 Msa1345070:CDS 25.0%
! CATTAGTGACATAAATAACA+TGG - tig0007057:63611-63630 None:intergenic 25.0%
! AGAAAGAAATGAAACTCTAA+AGG - tig0007057:63694-63713 None:intergenic 25.0%
! ACAATGACAGTTTTCCATTT+TGG + tig0007057:62867-62886 Msa1345070:CDS 30.0%
CTTTATATACGAATAAGGCT+TGG + tig0007057:62909-62928 Msa1345070:CDS 30.0%
CAAACAAATATTAAGCAGGT+TGG + tig0007057:63190-63209 Msa1345070:intron 30.0%
ACTTCATCAAACACAAAAGT+TGG - tig0007057:63301-63320 None:intergenic 30.0%
AAAGTAATGTAATATGGCCT+AGG - tig0007057:63364-63383 None:intergenic 30.0%
! ACAATGACAGTTTTCCATTT+TGG + tig0007057:62867-62886 Msa1345070:CDS 30.0%
CTTTATATACGAATAAGGCT+TGG + tig0007057:62909-62928 Msa1345070:CDS 30.0%
CAAACAAATATTAAGCAGGT+TGG + tig0007057:63190-63209 Msa1345070:intron 30.0%
ACTTCATCAAACACAAAAGT+TGG - tig0007057:63301-63320 None:intergenic 30.0%
AAAGTAATGTAATATGGCCT+AGG - tig0007057:63364-63383 None:intergenic 30.0%
!!! ATTTTGAAACACGGAAGGAA+TGG - tig0007057:62620-62639 None:intergenic 35.0%
!!! TTGACATTTTGAAACACGGA+AGG - tig0007057:62625-62644 None:intergenic 35.0%
CAAAATGTCAAAATCGGAAC+AGG + tig0007057:62634-62653 Msa1345070:exon 35.0%
ACACGATGAAGATTCATATG+AGG + tig0007057:62697-62716 Msa1345070:CDS 35.0%
TGAAGATTCATATGAGGATG+AGG + tig0007057:62703-62722 Msa1345070:CDS 35.0%
CATGAAACAAGATTCATCTG+TGG + tig0007057:62742-62761 Msa1345070:CDS 35.0%
TCACTATACTCAGAATCTTC+TGG - tig0007057:62818-62837 None:intergenic 35.0%
AGAAGATTCTGAGTATAGTG+AGG + tig0007057:62817-62836 Msa1345070:CDS 35.0%
TATATACGAATAAGGCTTGG+AGG + tig0007057:62912-62931 Msa1345070:CDS 35.0%
ATATACGAATAAGGCTTGGA+GGG + tig0007057:62913-62932 Msa1345070:CDS 35.0%
CTACTTCTTTATATGCTCCT+AGG + tig0007057:63344-63363 Msa1345070:CDS 35.0%
TAAAGCAAAAGCAGAAGCAA+AGG + tig0007057:63384-63403 Msa1345070:CDS 35.0%
AAAGCAAAAGCAGAAGCAAA+GGG + tig0007057:63385-63404 Msa1345070:CDS 35.0%
ATGAAACACTCTTTGACAAC+AGG - tig0007057:63469-63488 None:intergenic 35.0%
AAATCTAATACACATCGCCA+TGG + tig0007057:63494-63513 Msa1345070:exon 35.0%
AACAAGCATAACCATAACCA+TGG - tig0007057:63514-63533 None:intergenic 35.0%
! GGTTATGGTTATGCTTGTTT+TGG + tig0007057:63515-63534 Msa1345070:three_prime_UTR 35.0%
!!! ATTTTGAAACACGGAAGGAA+TGG - tig0007057:62620-62639 None:intergenic 35.0%
!!! TTGACATTTTGAAACACGGA+AGG - tig0007057:62625-62644 None:intergenic 35.0%
CAAAATGTCAAAATCGGAAC+AGG + tig0007057:62634-62653 Msa1345070:exon 35.0%
ACACGATGAAGATTCATATG+AGG + tig0007057:62697-62716 Msa1345070:CDS 35.0%
TGAAGATTCATATGAGGATG+AGG + tig0007057:62703-62722 Msa1345070:CDS 35.0%
CATGAAACAAGATTCATCTG+TGG + tig0007057:62742-62761 Msa1345070:CDS 35.0%
TCACTATACTCAGAATCTTC+TGG - tig0007057:62818-62837 None:intergenic 35.0%
AGAAGATTCTGAGTATAGTG+AGG + tig0007057:62817-62836 Msa1345070:CDS 35.0%
TATATACGAATAAGGCTTGG+AGG + tig0007057:62912-62931 Msa1345070:CDS 35.0%
ATATACGAATAAGGCTTGGA+GGG + tig0007057:62913-62932 Msa1345070:CDS 35.0%
CTACTTCTTTATATGCTCCT+AGG + tig0007057:63344-63363 Msa1345070:CDS 35.0%
TAAAGCAAAAGCAGAAGCAA+AGG + tig0007057:63384-63403 Msa1345070:CDS 35.0%
AAAGCAAAAGCAGAAGCAAA+GGG + tig0007057:63385-63404 Msa1345070:CDS 35.0%
ATGAAACACTCTTTGACAAC+AGG - tig0007057:63469-63488 None:intergenic 35.0%
AAATCTAATACACATCGCCA+TGG + tig0007057:63494-63513 Msa1345070:exon 35.0%
AACAAGCATAACCATAACCA+TGG - tig0007057:63514-63533 None:intergenic 35.0%
! GGTTATGGTTATGCTTGTTT+TGG + tig0007057:63515-63534 Msa1345070:three_prime_UTR 35.0%
AACAGGAACCGAAATCGAAA+CGG + tig0007057:62651-62670 Msa1345070:CDS 40.0%
GATTTCAGCCGTTTCGATTT+CGG - tig0007057:62662-62681 None:intergenic 40.0%
GAAATCGAAACGGCTGAAAT+CGG + tig0007057:62661-62680 Msa1345070:CDS 40.0%
AAATCGAAACGGCTGAAATC+GGG + tig0007057:62662-62681 Msa1345070:CDS 40.0%
AGATTCATATGAGGATGAGG+AGG + tig0007057:62706-62725 Msa1345070:CDS 40.0%
GAAACAAGATTCATCTGTGG+AGG + tig0007057:62745-62764 Msa1345070:CDS 40.0%
TACTCAGAATCTTCTGGATC+CGG - tig0007057:62812-62831 None:intergenic 40.0%
TCACGACGCTTATACCAAAA+TGG - tig0007057:62884-62903 None:intergenic 40.0%
!! TTTTGGTATAAGCGTCGTGA+TGG + tig0007057:62884-62903 Msa1345070:CDS 40.0%
GATTCTGGACAATACAAAGC+AGG + tig0007057:63048-63067 Msa1345070:intron 40.0%
TAAAGAAGTAGCACCAGGAT+GGG - tig0007057:63335-63354 None:intergenic 40.0%
ATAAAGAAGTAGCACCAGGA+TGG - tig0007057:63336-63355 None:intergenic 40.0%
GCATATAAAGAAGTAGCACC+AGG - tig0007057:63340-63359 None:intergenic 40.0%
AATACACATCGCCATGGTTA+TGG + tig0007057:63500-63519 Msa1345070:exon 40.0%
!!! ATGCTTGTTTTGGTTTGCCT+TGG + tig0007057:63525-63544 Msa1345070:three_prime_UTR 40.0%
AACAGGAACCGAAATCGAAA+CGG + tig0007057:62651-62670 Msa1345070:CDS 40.0%
GATTTCAGCCGTTTCGATTT+CGG - tig0007057:62662-62681 None:intergenic 40.0%
GAAATCGAAACGGCTGAAAT+CGG + tig0007057:62661-62680 Msa1345070:CDS 40.0%
AAATCGAAACGGCTGAAATC+GGG + tig0007057:62662-62681 Msa1345070:CDS 40.0%
AGATTCATATGAGGATGAGG+AGG + tig0007057:62706-62725 Msa1345070:CDS 40.0%
GAAACAAGATTCATCTGTGG+AGG + tig0007057:62745-62764 Msa1345070:CDS 40.0%
TACTCAGAATCTTCTGGATC+CGG - tig0007057:62812-62831 None:intergenic 40.0%
TCACGACGCTTATACCAAAA+TGG - tig0007057:62884-62903 None:intergenic 40.0%
!! TTTTGGTATAAGCGTCGTGA+TGG + tig0007057:62884-62903 Msa1345070:CDS 40.0%
GATTCTGGACAATACAAAGC+AGG + tig0007057:63048-63067 Msa1345070:intron 40.0%
TAAAGAAGTAGCACCAGGAT+GGG - tig0007057:63335-63354 None:intergenic 40.0%
ATAAAGAAGTAGCACCAGGA+TGG - tig0007057:63336-63355 None:intergenic 40.0%
GCATATAAAGAAGTAGCACC+AGG - tig0007057:63340-63359 None:intergenic 40.0%
AATACACATCGCCATGGTTA+TGG + tig0007057:63500-63519 Msa1345070:exon 40.0%
!!! ATGCTTGTTTTGGTTTGCCT+TGG + tig0007057:63525-63544 Msa1345070:three_prime_UTR 40.0%
ACAAGATTCATCTGTGGAGG+AGG + tig0007057:62748-62767 Msa1345070:CDS 45.0%
GAATCTTCTGGATCCGGTTT+CGG - tig0007057:62806-62825 None:intergenic 45.0%
TGAGTATAGTGAGGAAGACG+AGG + tig0007057:62826-62845 Msa1345070:CDS 45.0%
CGCTCTAGCAAACTAAGTGT+AGG + tig0007057:62968-62987 Msa1345070:intron 45.0%
ACATCATCGCTTAAGTCGAG+GGG - tig0007057:63121-63140 None:intergenic 45.0%
CACATCATCGCTTAAGTCGA+GGG - tig0007057:63122-63141 None:intergenic 45.0%
TTCAAAGCAGCATTGCAGAG+GGG - tig0007057:63160-63179 None:intergenic 45.0%
CTTCAAAGCAGCATTGCAGA+GGG - tig0007057:63161-63180 None:intergenic 45.0%
TCTTCAAAGCAGCATTGCAG+AGG - tig0007057:63162-63181 None:intergenic 45.0%
GTCTGGAAAGTTGTTAGAGC+AGG - tig0007057:63418-63437 None:intergenic 45.0%
TACGCTGCAATACATGAGTC+TGG - tig0007057:63435-63454 None:intergenic 45.0%
ACTACACGTAAGTCTTGCCA+AGG - tig0007057:63545-63564 None:intergenic 45.0%
ACAAGATTCATCTGTGGAGG+AGG + tig0007057:62748-62767 Msa1345070:CDS 45.0%
GAATCTTCTGGATCCGGTTT+CGG - tig0007057:62806-62825 None:intergenic 45.0%
TGAGTATAGTGAGGAAGACG+AGG + tig0007057:62826-62845 Msa1345070:CDS 45.0%
CGCTCTAGCAAACTAAGTGT+AGG + tig0007057:62968-62987 Msa1345070:intron 45.0%
ACATCATCGCTTAAGTCGAG+GGG - tig0007057:63121-63140 None:intergenic 45.0%
CACATCATCGCTTAAGTCGA+GGG - tig0007057:63122-63141 None:intergenic 45.0%
TTCAAAGCAGCATTGCAGAG+GGG - tig0007057:63160-63179 None:intergenic 45.0%
CTTCAAAGCAGCATTGCAGA+GGG - tig0007057:63161-63180 None:intergenic 45.0%
TCTTCAAAGCAGCATTGCAG+AGG - tig0007057:63162-63181 None:intergenic 45.0%
GTCTGGAAAGTTGTTAGAGC+AGG - tig0007057:63418-63437 None:intergenic 45.0%
TACGCTGCAATACATGAGTC+TGG - tig0007057:63435-63454 None:intergenic 45.0%
ACTACACGTAAGTCTTGCCA+AGG - tig0007057:63545-63564 None:intergenic 45.0%
!! TTACTTATTTATTATTAATT+AGG + tig0007057:63273-63292 Msa1345070:intron 5.0%
!! TACTTATTTATTATTAATTA+GGG + tig0007057:63274-63293 Msa1345070:intron 5.0%
!! AATTAATTAAATAAATATGA+TGG + tig0007057:63566-63585 Msa1345070:three_prime_UTR 5.0%
!! ATTAATTAAATAAATATGAT+GGG + tig0007057:63567-63586 Msa1345070:three_prime_UTR 5.0%
!! TTACTTATTTATTATTAATT+AGG + tig0007057:63273-63292 Msa1345070:intron 5.0%
!! TACTTATTTATTATTAATTA+GGG + tig0007057:63274-63293 Msa1345070:intron 5.0%
!! AATTAATTAAATAAATATGA+TGG + tig0007057:63566-63585 Msa1345070:three_prime_UTR 5.0%
!! ATTAATTAAATAAATATGAT+GGG + tig0007057:63567-63586 Msa1345070:three_prime_UTR 5.0%
GAAACGGCTGAAATCGGGAT+CGG + tig0007057:62667-62686 Msa1345070:CDS 50.0%
AGATTCATCTGTGGAGGAGG+AGG + tig0007057:62751-62770 Msa1345070:CDS 50.0%
GGAGGAGAAACGGCTGAAAT+CGG + tig0007057:62778-62797 Msa1345070:CDS 50.0%
GCTGAAATCGGAACCGAAAC+CGG + tig0007057:62790-62809 Msa1345070:CDS 50.0%
GCACATCATCGCTTAAGTCG+AGG - tig0007057:63123-63142 None:intergenic 50.0%
TGCAGAGGGGTTTGAGAGTA+CGG - tig0007057:63147-63166 None:intergenic 50.0%
GTTGTCAAGTGTACCCATCC+TGG + tig0007057:63319-63338 Msa1345070:CDS 50.0%
GCAAAAGCAGAAGCAAAGGG+AGG + tig0007057:63388-63407 Msa1345070:CDS 50.0%
GAAACGGCTGAAATCGGGAT+CGG + tig0007057:62667-62686 Msa1345070:CDS 50.0%
AGATTCATCTGTGGAGGAGG+AGG + tig0007057:62751-62770 Msa1345070:CDS 50.0%
GGAGGAGAAACGGCTGAAAT+CGG + tig0007057:62778-62797 Msa1345070:CDS 50.0%
GCTGAAATCGGAACCGAAAC+CGG + tig0007057:62790-62809 Msa1345070:CDS 50.0%
GCACATCATCGCTTAAGTCG+AGG - tig0007057:63123-63142 None:intergenic 50.0%
TGCAGAGGGGTTTGAGAGTA+CGG - tig0007057:63147-63166 None:intergenic 50.0%
GTTGTCAAGTGTACCCATCC+TGG + tig0007057:63319-63338 Msa1345070:CDS 50.0%
GCAAAAGCAGAAGCAAAGGG+AGG + tig0007057:63388-63407 Msa1345070:CDS 50.0%
TTCATCTGTGGAGGAGGAGG+AGG + tig0007057:62754-62773 Msa1345070:CDS 55.0%
AGGAGGAGGAGGAGGAGAAA+CGG + tig0007057:62768-62787 Msa1345070:CDS 55.0%
TTCATCTGTGGAGGAGGAGG+AGG + tig0007057:62754-62773 Msa1345070:CDS 55.0%
AGGAGGAGGAGGAGGAGAAA+CGG + tig0007057:62768-62787 Msa1345070:CDS 55.0%
ATCTGTGGAGGAGGAGGAGG+AGG + tig0007057:62757-62776 Msa1345070:CDS 60.0%
CACTGTCCACCACGTTGTGC+CGG + tig0007057:63085-63104 Msa1345070:CDS 60.0%
ATCTGTGGAGGAGGAGGAGG+AGG + tig0007057:62757-62776 Msa1345070:CDS 60.0%
CACTGTCCACCACGTTGTGC+CGG + tig0007057:63085-63104 Msa1345070:CDS 60.0%
TGTGGAGGAGGAGGAGGAGG+AGG + tig0007057:62760-62779 Msa1345070:CDS 65.0%
!! CGGCACAACGTGGTGGACAG+TGG - tig0007057:63087-63106 None:intergenic 65.0%
TGTCCACCACGTTGTGCCGG+CGG + tig0007057:63088-63107 Msa1345070:CDS 65.0%
ATACCGCCGGCACAACGTGG+TGG - tig0007057:63094-63113 None:intergenic 65.0%
ACGATACCGCCGGCACAACG+TGG - tig0007057:63097-63116 None:intergenic 65.0%
! GTCGAGGGGTACGATACCGC+CGG - tig0007057:63107-63126 None:intergenic 65.0%
TGTGGAGGAGGAGGAGGAGG+AGG + tig0007057:62760-62779 Msa1345070:CDS 65.0%
!! CGGCACAACGTGGTGGACAG+TGG - tig0007057:63087-63106 None:intergenic 65.0%
TGTCCACCACGTTGTGCCGG+CGG + tig0007057:63088-63107 Msa1345070:CDS 65.0%
ATACCGCCGGCACAACGTGG+TGG - tig0007057:63094-63113 None:intergenic 65.0%
ACGATACCGCCGGCACAACG+TGG - tig0007057:63097-63116 None:intergenic 65.0%
! GTCGAGGGGTACGATACCGC+CGG - tig0007057:63107-63126 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0007057 gene 62615 63803 62615 ID=Msa1345070;Name=Msa1345070
tig0007057 mRNA 62615 63803 62615 ID=Msa1345070-mRNA-1;Parent=Msa1345070;Name=Msa1345070-mRNA-1;_AED=0.35;_eAED=0.35;_QI=23|1|1|1|1|1|3|302|230
tig0007057 exon 62615 62985 62615 ID=Msa1345070-mRNA-1:exon:4792;Parent=Msa1345070-mRNA-1
tig0007057 exon 63070 63207 63070 ID=Msa1345070-mRNA-1:exon:4793;Parent=Msa1345070-mRNA-1
tig0007057 exon 63295 63803 63295 ID=Msa1345070-mRNA-1:exon:4794;Parent=Msa1345070-mRNA-1
tig0007057 five_prime_UTR 62615 62637 62615 ID=Msa1345070-mRNA-1:five_prime_utr;Parent=Msa1345070-mRNA-1
tig0007057 CDS 62638 62985 62638 ID=Msa1345070-mRNA-1:cds;Parent=Msa1345070-mRNA-1
tig0007057 CDS 63070 63207 63070 ID=Msa1345070-mRNA-1:cds;Parent=Msa1345070-mRNA-1
tig0007057 CDS 63295 63501 63295 ID=Msa1345070-mRNA-1:cds;Parent=Msa1345070-mRNA-1
tig0007057 three_prime_UTR 63502 63803 63502 ID=Msa1345070-mRNA-1:three_prime_utr;Parent=Msa1345070-mRNA-1
Gene Sequence

>Msa1345070

ATGTCAAAATCGGAACAGGAACCGAAATCGAAACGGCTGAAATCGGGATCGGAACAGAAACACGATGAAGATTCATATGAGGATGAGGAGGATAAGCAAGAATTCATGAAACAAGATTCATCTGTGGAGGAGGAGGAGGAGGAGGAGAAACGGCTGAAATCGGAACCGAAACCGGATCCAGAAGATTCTGAGTATAGTGAGGAAGACGAGGATAACTTGTCTATGCATGACAATGACAGTTTTCCATTTTGGTATAAGCGTCGTGATGGTTCTTTATATACGAATAAGGCTTGGAGGGATCATAAGACTAAAATTCTTGACTATAAAAATCGCTCTAGCAAACTAAGTGTCTACGATGCTATCCACTGTCCACCACGTTGTGCCGGCGGTATCGTACCCCTCGACTTAAGCGATGATGTGCTCCGTACTCTCAAACCCCTCTGCAATGCTGCTTTGAAGATTTACAATCAAACAAATATTAAGCAGGGTCCAACTTTTGTGTTTGATGAAGTTGTCAAGTGTACCCATCCTGGTGCTACTTCTTTATATGCTCCTAGGCCATATTACATTACTTTTAAAGCAAAAGCAGAAGCAAAGGGAGGAAGACCTGCTCTAACAACTTTCCAGACTCATGTATTGCAGCGTAAAGATAAAGATCCTGTTGTCAAAGAGTGTTTCATTAAAAAAATCTAA
Protein sequence

>Msa1345070

MSKSEQEPKSKRLKSGSEQKHDEDSYEDEEDKQEFMKQDSSVEEEEEEEKRLKSEPKPDPEDSEYSEEDEDNLSMHDNDSFPFWYKRRDGSLYTNKAWRDHKTKILDYKNRSSKLSVYDAIHCPPRCAGGIVPLDLSDDVLRTLKPLCNAALKIYNQTNIKQGPTFVFDEVVKCTHPGATSLYAPRPYYITFKAKAEAKGGRPALTTFQTHVLQRKDKDPVVKECFIKKI*