AlfalfaGEDB | Msa1345070

M. sativa cultivar zhongmu-4 / Msa1345070

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345070	KEH19492.1	89.062	192	13	2	47	230	9	200	8.59e-121	352

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345070	A0A072TQ21	89.062	192	13	2	47	230	9	200	4.10e-121	352

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0183460	Msa1345070	0.825757	3.938717e-54	-8.615850e-47
Msa0189520	Msa1345070	0.801680	8.031479e-49	-8.615850e-47
Msa0207670	Msa1345070	0.805749	1.148889e-49	-8.615850e-47
Msa0631350	Msa1345070	0.815786	7.735331e-52	-8.615850e-47
Msa0669720	Msa1345070	0.801644	8.168401e-49	-8.615850e-47
Msa1133230	Msa1345070	0.809856	1.538915e-50	-8.615850e-47
Msa1133250	Msa1345070	0.825169	5.427957e-54	-8.615850e-47
Msa1296830	Msa1345070	0.827347	1.645067e-54	-8.615850e-47
Msa1345040	Msa1345070	0.819732	9.949862e-53	-8.615850e-47
Msa1345070	Msa1351540	0.802744	4.850523e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345070	MtrunA17_Chr4g0027321	55.172	174	61	6	61	228	130	292	2.02e-49	163
Msa1345070	MtrunA17_Chr3g0119711	40.191	209	99	10	33	228	3	198	1.47e-29	109
Msa1345070	MtrunA17_Chr3g0120641	36.199	221	116	8	28	228	26	241	2.73e-20	86.3
Msa1345070	MtrunA17_Chr3g0120941	36.139	202	103	10	40	228	54	242	9.55e-20	84.7
Msa1345070	MtrunA17_Chr3g0120951	37.278	169	84	9	69	228	51	206	9.99e-19	81.3
Msa1345070	MtrunA17_Chr3g0120981	36.667	210	99	12	28	228	21	205	3.23e-17	77.0
Msa1345070	MtrunA17_Chr3g0120591	36.025	161	95	4	73	228	65	222	6.14e-17	76.6
Msa1345070	MtrunA17_Chr3g0120961	35.897	156	73	7	75	222	47	183	1.30e-13	67.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 67 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAAAAGCAGAAGCAAA+GGG	0.538089	tig0007057:+63385	Msa1345070:CDS
AAAGTAATGTAATATGGCCT+AGG	0.487724	tig0007057:-63361	None:intergenic
AAATCGAAACGGCTGAAATC+GGG	0.418006	tig0007057:+62662	Msa1345070:CDS
AAATCTAATACACATCGCCA+TGG	0.628062	tig0007057:+63494	Msa1345070:exon
AACAAGCATAACCATAACCA+TGG	0.658373	tig0007057:-63511	None:intergenic
AACAGGAACCGAAATCGAAA+CGG	0.558710	tig0007057:+62651	Msa1345070:CDS
AATACACATCGCCATGGTTA+TGG	0.379705	tig0007057:+63500	Msa1345070:exon
AATTAATTAAATAAATATGA+TGG	0.373275	tig0007057:+63566	Msa1345070:three_prime_UTR
ACAAGATTCATCTGTGGAGG+AGG	0.546974	tig0007057:+62748	Msa1345070:CDS
ACACGATGAAGATTCATATG+AGG	0.514467	tig0007057:+62697	Msa1345070:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTTATATACGAATAAGGCT+TGG	+	tig0007057:62909-62928	Msa1345070:CDS	30.0%
CAAACAAATATTAAGCAGGT+TGG	+	tig0007057:63190-63209	Msa1345070:intron	30.0%
ACTTCATCAAACACAAAAGT+TGG	-	tig0007057:63301-63320	None:intergenic	30.0%
AAAGTAATGTAATATGGCCT+AGG	-	tig0007057:63364-63383	None:intergenic	30.0%
CTTTATATACGAATAAGGCT+TGG	+	tig0007057:62909-62928	Msa1345070:CDS	30.0%
CAAACAAATATTAAGCAGGT+TGG	+	tig0007057:63190-63209	Msa1345070:intron	30.0%
ACTTCATCAAACACAAAAGT+TGG	-	tig0007057:63301-63320	None:intergenic	30.0%
AAAGTAATGTAATATGGCCT+AGG	-	tig0007057:63364-63383	None:intergenic	30.0%
CAAAATGTCAAAATCGGAAC+AGG	+	tig0007057:62634-62653	Msa1345070:exon	35.0%
ACACGATGAAGATTCATATG+AGG	+	tig0007057:62697-62716	Msa1345070:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0007057	gene	62615	63803	62615	ID=Msa1345070;Name=Msa1345070
tig0007057	mRNA	62615	63803	62615	ID=Msa1345070-mRNA-1;Parent=Msa1345070;Name=Msa1345070-mRNA-1;_AED=0.35;_eAED=0.35;_QI=23\|1\|1\|1\|1\|1\|3\|302\|230
tig0007057	exon	62615	62985	62615	ID=Msa1345070-mRNA-1:exon:4792;Parent=Msa1345070-mRNA-1
tig0007057	exon	63070	63207	63070	ID=Msa1345070-mRNA-1:exon:4793;Parent=Msa1345070-mRNA-1
tig0007057	exon	63295	63803	63295	ID=Msa1345070-mRNA-1:exon:4794;Parent=Msa1345070-mRNA-1
tig0007057	five_prime_UTR	62615	62637	62615	ID=Msa1345070-mRNA-1:five_prime_utr;Parent=Msa1345070-mRNA-1
tig0007057	CDS	62638	62985	62638	ID=Msa1345070-mRNA-1:cds;Parent=Msa1345070-mRNA-1
tig0007057	CDS	63070	63207	63070	ID=Msa1345070-mRNA-1:cds;Parent=Msa1345070-mRNA-1
tig0007057	CDS	63295	63501	63295	ID=Msa1345070-mRNA-1:cds;Parent=Msa1345070-mRNA-1
tig0007057	three_prime_UTR	63502	63803	63502	ID=Msa1345070-mRNA-1:three_prime_utr;Parent=Msa1345070-mRNA-1

Gene Sequence

>Msa1345070

ATGTCAAAATCGGAACAGGAACCGAAATCGAAACGGCTGAAATCGGGATCGGAACAGAAACACGATGAAGATTCATATGAGGATGAGGAGGATAAGCAAGAATTCATGAAACAAGATTCATCTGTGGAGGAGGAGGAGGAGGAGGAGAAACGGCTGAAATCGGAACCGAAACCGGATCCAGAAGATTCTGAGTATAGTGAGGAAGACGAGGATAACTTGTCTATGCATGACAATGACAGTTTTCCATTTTGGTATAAGCGTCGTGATGGTTCTTTATATACGAATAAGGCTTGGAGGGATCATAAGACTAAAATTCTTGACTATAAAAATCGCTCTAGCAAACTAAGTGTCTACGATGCTATCCACTGTCCACCACGTTGTGCCGGCGGTATCGTACCCCTCGACTTAAGCGATGATGTGCTCCGTACTCTCAAACCCCTCTGCAATGCTGCTTTGAAGATTTACAATCAAACAAATATTAAGCAGGGTCCAACTTTTGTGTTTGATGAAGTTGTCAAGTGTACCCATCCTGGTGCTACTTCTTTATATGCTCCTAGGCCATATTACATTACTTTTAAAGCAAAAGCAGAAGCAAAGGGAGGAAGACCTGCTCTAACAACTTTCCAGACTCATGTATTGCAGCGTAAAGATAAAGATCCTGTTGTCAAAGAGTGTTTCATTAAAAAAATCTAA

Protein sequence

>Msa1345070

MSKSEQEPKSKRLKSGSEQKHDEDSYEDEEDKQEFMKQDSSVEEEEEEEKRLKSEPKPDPEDSEYSEEDEDNLSMHDNDSFPFWYKRRDGSLYTNKAWRDHKTKILDYKNRSSKLSVYDAIHCPPRCAGGIVPLDLSDDVLRTLKPLCNAALKIYNQTNIKQGPTFVFDEVVKCTHPGATSLYAPRPYYITFKAKAEAKGGRPALTTFQTHVLQRKDKDPVVKECFIKKI*