AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1133230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1133230 MtrunA17_Chr1g0176041 95.849 265 11 0 6 270 344 608 0.0 530
Msa1133230 MtrunA17_Chr3g0121351 73.381 278 61 2 6 270 336 613 5.43e-124 366
Msa1133230 MtrunA17_Chr4g0063551 65.158 221 70 2 16 232 346 563 4.24e-92 285
Msa1133230 MtrunA17_Chr2g0330401 42.273 220 106 6 16 227 287 493 5.50e-44 155
Msa1133230 MtrunA17_Chr4g0076491 43.077 195 104 2 15 209 497 684 2.02e-43 156
Msa1133230 MtrunA17_Chr3g0107851 44.706 170 92 1 16 183 309 478 3.17e-42 151
Msa1133230 MtrunA17_Chr1g0157691 41.053 190 103 2 16 204 347 528 2.34e-41 149
Msa1133230 MtrunA17_Chr6g0449741 42.784 194 102 3 16 209 460 644 3.20e-41 150
Msa1133230 MtrunA17_Chr6g0449911 38.462 221 126 4 6 223 345 558 1.78e-40 148
Msa1133230 MtrunA17_Chr1g0200181 41.053 190 103 2 16 204 345 526 2.68e-40 147
Msa1133230 MtrunA17_Chr2g0293631 39.906 213 118 4 16 226 686 890 2.30e-38 142
Msa1133230 MtrunA17_Chr5g0409981 41.038 212 104 6 6 207 285 485 7.34e-35 131
Msa1133230 MtrunA17_Chr7g0270131 39.130 207 116 4 16 220 423 621 1.05e-31 123
Msa1133230 MtrunA17_Chr6g0461751 30.952 210 137 3 14 220 330 534 3.04e-30 118
Msa1133230 MtrunA17_Chr7g0263771 33.333 186 118 1 20 205 323 502 1.17e-29 117
Msa1133230 MtrunA17_Chr7g0271121 36.170 188 107 4 6 189 288 466 1.04e-28 114
Msa1133230 MtrunA17_Chr2g0277821 41.860 129 75 0 73 201 749 877 1.63e-27 111
Msa1133230 MtrunA17_Chr3g0137901 42.424 132 69 1 51 182 25 149 2.70e-27 103
Msa1133230 MtrunA17_Chr1g0149251 42.424 132 69 1 51 182 189 313 1.61e-25 103
Msa1133230 MtrunA17_Chr1g0193491 34.685 222 107 8 38 232 320 530 1.69e-25 105
Msa1133230 MtrunA17_Chr5g0442191 45.872 109 58 1 71 179 322 429 1.52e-24 102
Msa1133230 MtrunA17_Chr2g0297261 37.097 124 78 0 78 201 276 399 1.85e-24 101
Msa1133230 MtrunA17_Chr4g0003441 41.221 131 70 1 51 181 333 456 8.12e-24 100
Msa1133230 MtrunA17_Chr2g0300291 46.018 113 61 0 78 190 390 502 1.89e-23 99.8
Msa1133230 MtrunA17_Chr2g0310581 34.641 153 96 1 42 194 268 416 2.22e-23 99.0
Msa1133230 MtrunA17_Chr7g0236841 30.000 240 150 5 14 245 197 426 3.08e-23 98.2
Msa1133230 MtrunA17_Chr3g0095031 47.115 104 54 1 71 174 331 433 3.69e-23 98.6
Msa1133230 MtrunA17_Chr6g0483491 32.474 194 121 3 9 202 195 378 5.33e-23 97.4
Msa1133230 MtrunA17_Chr3g0098121 34.737 190 109 3 1 190 338 512 1.06e-22 97.4
Msa1133230 MtrunA17_Chr4g0070981 33.333 126 84 0 77 202 282 407 5.05e-22 94.4
Msa1133230 MtrunA17_Chr2g0299681 32.540 126 85 0 77 202 282 407 4.08e-21 92.0
Msa1133230 MtrunA17_Chr2g0329931 35.659 129 77 2 77 202 282 407 5.25e-21 91.7
Msa1133230 MtrunA17_Chr8g0342861 34.109 129 79 2 77 202 282 407 8.00e-21 90.9
Msa1133230 MtrunA17_Chr3g0114081 37.086 151 82 4 34 178 241 384 1.14e-19 88.2
Msa1133230 MtrunA17_Chr4g0040761 35.036 137 79 2 61 189 250 384 1.32e-19 88.2
Msa1133230 MtrunA17_Chr6g0475251 42.105 114 60 2 75 187 357 465 2.12e-19 87.8
Msa1133230 MtrunA17_Chr1g0204391 41.739 115 61 2 74 187 313 422 2.70e-19 87.4
Msa1133230 MtrunA17_Chr7g0236831 32.000 200 123 3 51 250 459 645 6.14e-19 86.3
Msa1133230 MtrunA17_Chr8g0364251 33.951 162 95 5 42 198 400 554 1.63e-18 85.1
Msa1133230 MtrunA17_Chr4g0025141 38.261 115 58 2 75 179 84 195 8.25e-18 80.5
Msa1133230 MtrunA17_Chr4g0058591 37.778 135 77 3 16 149 393 521 3.37e-17 80.9
Msa1133230 MtrunA17_Chr8g0342851 32.800 125 84 0 78 202 189 313 3.42e-17 79.7
Msa1133230 MtrunA17_Chr6g0466611 35.556 135 82 4 78 210 403 534 1.58e-16 79.3
Msa1133230 MtrunA17_Chr2g0301281 38.372 86 53 0 104 189 388 473 2.81e-16 78.6
Msa1133230 MtrunA17_Chr4g0029191 33.913 115 73 1 104 218 391 502 2.92e-16 78.2
Msa1133230 MtrunA17_Chr1g0179761 36.975 119 58 2 75 179 332 447 1.21e-15 76.3
Msa1133230 MtrunA17_Chr4g0034411 28.507 221 131 6 6 207 257 469 1.46e-15 76.3
Msa1133230 MtrunA17_Chr4g0058601 46.269 67 36 0 139 205 9 75 1.14e-14 68.9
Msa1133230 MtrunA17_Chr2g0309551 33.613 119 79 0 76 194 408 526 3.05e-14 72.4
Msa1133230 MtrunA17_Chr7g0247191 30.159 126 82 2 50 175 368 487 8.04e-14 70.9
Msa1133230 MtrunA17_Chr7g0247181 30.159 126 82 2 50 175 358 477 8.41e-14 70.9
Msa1133230 MtrunA17_Chr7g0247221 30.159 126 82 2 50 175 361 480 9.01e-14 70.9
Msa1133230 MtrunA17_Chr5g0443991 28.959 221 130 8 6 207 257 469 9.14e-14 70.9
Msa1133230 MtrunA17_Chr2g0307471 40.625 96 50 2 95 189 364 453 2.78e-13 69.3
Msa1133230 MtrunA17_Chr1g0155201 34.483 145 77 6 114 244 162 302 3.43e-13 68.6
Msa1133230 MtrunA17_Chr8g0354761 32.432 111 75 0 73 183 254 364 6.42e-13 68.2
Msa1133230 MtrunA17_Chr8g0386451 31.613 155 96 4 56 200 527 681 9.33e-13 68.2
Msa1133230 MtrunA17_Chr8g0347161 36.752 117 71 1 56 172 344 457 3.60e-12 66.2
Msa1133230 MtrunA17_Chr5g0429221 38.614 101 59 1 79 179 251 348 4.21e-12 65.9
Msa1133230 MtrunA17_Chr4g0062301 43.284 67 38 0 101 167 371 437 7.90e-12 65.1
Msa1133230 MtrunA17_Chr8g0347151 42.697 89 48 1 104 189 488 576 9.93e-12 65.1
Msa1133230 MtrunA17_Chr4g0004301 36.250 80 51 0 110 189 372 451 8.70e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1133230 AT2G42520.2 83.251 203 34 0 16 218 360 562 3.39e-121 360
Msa1133230 AT2G42520.1 83.251 203 34 0 16 218 360 562 3.39e-121 360
Msa1133230 AT2G42520.3 83.251 203 34 0 16 218 360 562 3.39e-121 360
Msa1133230 AT3G58570.1 80.751 213 41 0 6 218 337 549 2.88e-120 358
Msa1133230 AT3G58510.1 75.109 229 53 2 6 234 342 566 6.76e-113 338
Msa1133230 AT3G58510.3 75.109 229 53 2 6 234 342 566 6.76e-113 338
Msa1133230 AT3G58510.2 75.109 229 53 2 6 234 342 566 6.76e-113 338
Msa1133230 AT1G55150.1 41.743 218 109 5 16 225 291 498 2.45e-43 154
Msa1133230 AT5G63120.2 44.103 195 94 4 16 205 357 541 2.89e-43 155
Msa1133230 AT1G55150.2 41.743 218 109 5 16 225 316 523 3.62e-43 154
Msa1133230 AT5G14610.7 40.777 206 111 4 16 219 221 417 7.43e-41 147
Msa1133230 AT5G14610.3 40.777 206 111 4 16 219 221 417 7.43e-41 147
Msa1133230 AT5G14610.6 40.777 206 111 4 16 219 352 548 1.19e-40 149
Msa1133230 AT5G14610.2 40.777 206 111 4 16 219 352 548 1.33e-40 148
Msa1133230 AT5G14610.5 40.777 206 111 4 16 219 360 556 1.38e-40 148
Msa1133230 AT5G14610.1 40.777 206 111 4 16 219 420 616 2.18e-40 148
Msa1133230 AT2G33730.1 40.722 194 108 2 16 209 525 711 7.38e-40 147
Msa1133230 AT3G01540.1 40.291 206 112 4 16 219 349 545 1.39e-39 145
Msa1133230 AT3G01540.4 40.291 206 112 4 16 219 349 545 1.43e-39 145
Msa1133230 AT3G01540.2 40.291 206 112 4 16 219 349 545 1.43e-39 145
Msa1133230 AT3G01540.3 40.291 206 112 4 16 219 349 545 1.43e-39 145
Msa1133230 AT5G51280.1 40.000 190 105 2 16 204 347 528 8.03e-39 143
Msa1133230 AT4G33370.1 39.267 191 105 3 16 204 298 479 3.71e-38 140
Msa1133230 AT3G06480.1 38.579 197 112 3 16 210 626 815 4.52e-36 136
Msa1133230 AT1G31970.1 41.624 197 103 4 16 207 310 499 9.91e-36 134
Msa1133230 AT1G28180.1 38.384 198 112 3 13 209 165 353 1.17e-31 120
Msa1133230 AT1G20920.2 36.788 193 116 1 16 208 386 572 5.22e-31 122
Msa1133230 AT1G20920.4 37.097 186 111 1 16 201 724 903 3.44e-30 119
Msa1133230 AT1G20920.6 37.097 186 111 1 16 201 724 903 3.44e-30 119
Msa1133230 AT1G20920.3 37.097 186 111 1 16 201 724 903 3.44e-30 119
Msa1133230 AT1G20920.7 37.097 186 111 1 16 201 724 903 3.44e-30 119
Msa1133230 AT1G20920.5 37.097 186 111 1 16 201 724 903 3.44e-30 119
Msa1133230 AT1G20920.1 37.097 186 111 1 16 201 724 903 3.44e-30 119
Msa1133230 AT2G47330.1 37.056 197 116 3 16 210 420 610 5.32e-30 119
Msa1133230 AT5G60990.1 33.184 223 130 3 37 247 230 445 3.42e-27 109
Msa1133230 AT3G09720.2 30.198 202 134 2 4 204 315 510 5.55e-27 109
Msa1133230 AT3G09720.1 30.198 202 134 2 4 204 315 510 5.55e-27 109
Msa1133230 AT5G26742.3 43.478 138 71 2 52 189 242 372 1.84e-26 108
Msa1133230 AT5G26742.1 36.170 188 107 4 6 189 287 465 2.31e-26 108
Msa1133230 AT5G26742.2 36.170 188 107 4 6 189 287 465 2.31e-26 108
Msa1133230 AT3G22330.1 40.909 132 77 1 71 202 346 476 1.13e-25 106
Msa1133230 AT3G22310.2 50.962 104 50 1 71 174 358 460 1.79e-25 105
Msa1133230 AT3G22310.1 50.962 104 50 1 71 174 358 460 1.84e-25 105
Msa1133230 AT5G11170.2 43.307 127 65 1 51 177 189 308 1.40e-24 100
Msa1133230 AT3G02065.1 28.079 203 140 2 16 217 167 364 1.64e-24 101
Msa1133230 AT5G11200.3 43.307 127 65 1 51 177 313 432 3.24e-24 101
Msa1133230 AT5G11170.1 43.307 127 65 1 51 177 272 391 3.46e-24 100
Msa1133230 AT5G11200.1 43.307 127 65 1 51 177 272 391 3.46e-24 100
Msa1133230 AT5G11200.2 43.307 127 65 1 51 177 331 450 3.59e-24 101
Msa1133230 AT1G51380.1 32.298 161 102 2 47 205 237 392 4.08e-24 100
Msa1133230 AT3G19760.1 34.677 124 81 0 78 201 278 401 7.68e-24 99.8
Msa1133230 AT3G02065.3 28.079 203 140 2 16 217 304 501 1.31e-23 100
Msa1133230 AT3G02065.2 28.079 203 140 2 16 217 304 501 1.31e-23 100
Msa1133230 AT1G16280.1 37.162 148 89 3 38 185 270 413 4.51e-23 98.2
Msa1133230 AT4G16630.1 34.211 190 110 3 1 190 354 528 1.30e-22 97.4
Msa1133230 AT1G72730.1 33.333 126 84 0 77 202 283 408 1.50e-21 93.6
Msa1133230 AT3G13920.1 33.333 126 84 0 77 202 281 406 3.93e-21 92.4
Msa1133230 AT3G13920.3 33.333 126 84 0 77 202 271 396 4.07e-21 92.0
Msa1133230 AT3G13920.4 33.333 126 84 0 77 202 276 401 5.29e-21 92.0
Msa1133230 AT1G54270.1 33.333 126 84 0 77 202 281 406 7.49e-21 91.7
Msa1133230 AT1G54270.2 33.333 126 84 0 77 202 276 401 8.63e-21 91.3
Msa1133230 AT3G09620.1 33.871 186 88 3 16 201 591 741 1.42e-20 91.7
Msa1133230 AT3G09620.2 33.871 186 88 3 16 201 592 742 1.43e-20 91.7
Msa1133230 AT3G13920.2 36.697 109 69 0 77 185 281 389 4.21e-20 89.4
Msa1133230 AT3G13920.5 36.697 109 69 0 77 185 285 393 4.35e-20 89.4
Msa1133230 AT3G53110.1 40.870 115 58 2 72 179 338 449 2.33e-19 87.8
Msa1133230 AT5G63120.1 41.406 128 60 4 16 138 357 474 1.12e-18 85.5
Msa1133230 AT5G63120.3 41.406 128 60 4 16 138 357 474 1.49e-18 85.1
Msa1133230 AT5G63120.4 41.406 128 60 4 16 138 357 474 1.49e-18 85.1
Msa1133230 AT5G63120.5 41.406 128 60 4 16 138 357 474 1.49e-18 85.1
Msa1133230 AT1G77030.1 33.577 137 81 2 61 189 260 394 7.63e-18 83.6
Msa1133230 AT4G09730.1 37.143 140 80 4 78 211 374 511 1.01e-17 83.2
Msa1133230 AT5G62190.1 38.938 113 65 2 75 187 351 459 3.83e-17 81.3
Msa1133230 AT5G05450.1 37.410 139 74 4 46 178 251 382 5.44e-17 80.9
Msa1133230 AT2G40700.1 41.860 86 50 0 104 189 380 465 5.78e-17 80.9
Msa1133230 AT5G14610.4 35.433 127 73 3 16 140 360 479 6.20e-16 77.8
Msa1133230 AT1G71370.1 36.496 137 76 4 47 178 243 373 8.23e-16 77.4
Msa1133230 AT3G06480.2 35.821 134 77 3 16 147 626 752 1.58e-15 76.6
Msa1133230 AT1G59990.1 32.773 119 77 2 77 194 427 543 2.42e-14 73.2
Msa1133230 AT1G59990.2 32.773 119 77 2 77 194 427 543 2.44e-14 73.2
Msa1133230 AT4G00660.1 30.159 126 82 2 50 175 350 469 1.26e-13 70.9
Msa1133230 AT4G00660.2 30.159 126 82 2 50 175 350 469 1.26e-13 70.9
Msa1133230 AT3G61240.2 30.159 126 82 2 50 175 343 462 1.30e-13 70.9
Msa1133230 AT3G61240.1 30.159 126 82 2 50 175 343 462 1.30e-13 70.9
Msa1133230 AT2G45810.1 30.159 126 82 2 50 175 373 492 1.97e-13 70.1
Msa1133230 AT1G12770.2 36.957 92 58 0 98 189 431 522 3.05e-13 69.7
Msa1133230 AT1G12770.1 36.957 92 58 0 98 189 431 522 3.05e-13 69.7
Msa1133230 AT5G19210.1 45.312 64 35 0 104 167 214 277 4.73e-13 68.6
Msa1133230 AT5G19210.3 45.312 64 35 0 104 167 214 277 4.73e-13 68.6
Msa1133230 AT5G08620.1 42.697 89 48 1 104 189 368 456 7.96e-13 68.6
Msa1133230 AT5G19210.2 45.312 64 35 0 104 167 371 434 9.04e-13 68.2
Msa1133230 AT3G16840.1 28.947 152 97 3 37 184 468 612 9.29e-13 68.6
Msa1133230 AT3G16840.2 28.947 152 97 3 37 184 469 613 9.30e-13 68.6
Msa1133230 AT3G18600.1 36.667 90 57 0 78 167 338 427 1.47e-12 67.8
Msa1133230 AT1G63250.1 34.188 117 77 0 56 172 569 685 1.50e-12 67.8
Msa1133230 AT5G08610.1 37.168 113 61 2 104 206 670 782 1.82e-12 67.8
Msa1133230 AT2G07750.1 34.188 117 77 0 56 172 616 732 9.27e-12 65.5
Msa1133230 AT5G63630.1 35.897 117 65 2 104 210 342 458 1.41e-11 64.7
Msa1133230 AT5G63630.2 44.928 69 38 0 104 172 536 604 1.57e-11 64.7
Msa1133230 AT5G65900.1 37.113 97 60 1 71 167 398 493 4.89e-11 63.2
Msa1133230 AT4G15850.1 40.299 67 40 0 106 172 370 436 8.67e-11 62.4

Find 102 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGCTAGTTATATCTTTC+TGG 0.065066 7_3:-86459748 Msa1133230:CDS
TTAATATGTGCATGCTAATT+AGG 0.082344 7_3:+86457354 None:intergenic
TCTAATTAATTAACAGAGTT+TGG 0.265774 7_3:-86459896 Msa1133230:five_prime_UTR
AGTAAGGAATGCATGGATTT+AGG 0.296210 7_3:+86457577 None:intergenic
TAATATGTGCATGCTAATTA+GGG 0.298326 7_3:+86457355 None:intergenic
AACAGGACGAGCTGGTAAAA+TGG 0.309296 7_3:-86457992 Msa1133230:CDS
TTTCCTGCAACTTGCATTCA+TGG 0.338462 7_3:-86459336 Msa1133230:CDS
TGTAACAAGTGCTTGGGATT+AGG 0.346044 7_3:-86457692 Msa1133230:exon
GGGTGTAAACTATATTGTTT+TGG 0.348976 7_3:-86457472 Msa1133230:three_prime_UTR
ACCATACCCTCCGCCATAAC+TGG 0.352232 7_3:+86457720 None:intergenic
CACATAATCATCAATGTCAT+TGG 0.353409 7_3:+86458029 None:intergenic
ACATCCGTTGCCACTAGAAT+TGG 0.361842 7_3:+86458108 None:intergenic
CGCTAATTCATTCTCATGTT+GGG 0.367537 7_3:-86457657 Msa1133230:three_prime_UTR
GGTGGAAACGGTGGTGGTTA+TGG 0.369732 7_3:-86457751 Msa1133230:CDS
AACATCACGGCCACCAAAAC+GGG 0.371256 7_3:+86457813 None:intergenic
AATAAAGGAAATGATTACTA+TGG 0.381802 7_3:-86457772 Msa1133230:CDS
GACAAAGATTTCCTGTAGTA+AGG 0.382851 7_3:+86457561 None:intergenic
AGTTATATCTTTCTGGCTGT+TGG 0.386936 7_3:-86459741 Msa1133230:CDS
AGGTCTGACTTTAGTGTTTG+TGG 0.388354 7_3:-86459409 Msa1133230:CDS
TTTCTGGCTGTTGGAAGGGT+TGG 0.391022 7_3:-86459732 Msa1133230:CDS
CTTTGTCAATGATCCACAAC+TGG 0.399349 7_3:-86457545 Msa1133230:three_prime_UTR
GTGGTAGAAACAAGCGGACT+GGG 0.409015 7_3:-86457840 Msa1133230:CDS
TCGCCATGAATGCAAGTTGC+AGG 0.411185 7_3:+86459333 None:intergenic
AAGGAGAGCTCATATAATAA+AGG 0.419864 7_3:-86457787 Msa1133230:CDS
AGGAGTCCTTGCTGCATAAC+GGG 0.432387 7_3:+86457870 None:intergenic
CTCGTCCTGTTCTTCCTATC+CGG 0.432877 7_3:+86458004 None:intergenic
GCCAGTTATGGCGGAGGGTA+TGG 0.434353 7_3:-86457721 Msa1133230:CDS
GAACACTGCTTGTGTGTTAA+CGG 0.434525 7_3:-86459360 Msa1133230:CDS
AACACTGCTTGTGTGTTAAC+GGG 0.434919 7_3:-86459359 Msa1133230:CDS
CAAATTACTGTCATTGAAGA+AGG 0.435697 7_3:+86457957 None:intergenic
TAACATCACGGCCACCAAAA+CGG 0.440534 7_3:+86457812 None:intergenic
TCGCTAATTCATTCTCATGT+TGG 0.441868 7_3:-86457658 Msa1133230:three_prime_UTR
GTCATAGAAACTGTTTGCTT+AGG 0.442872 7_3:-86459946 None:intergenic
TATGGTGTTCCTGCCAGTTA+TGG 0.448820 7_3:-86457733 Msa1133230:CDS
GTAATTATTAATTAAGTGTA+GGG 0.450458 7_3:+86457304 None:intergenic
ATATCTTTCTGGCTGTTGGA+AGG 0.455309 7_3:-86459737 Msa1133230:CDS
CAATGACAGTAATTTGTCAA+TGG 0.461949 7_3:-86457950 Msa1133230:CDS
GGTAATTATTAATTAAGTGT+AGG 0.464235 7_3:+86457303 None:intergenic
ACAGGACGAGCTGGTAAAAT+GGG 0.466923 7_3:-86457991 Msa1133230:CDS
GTCAAGGTGTAACAAGTGCT+TGG 0.472671 7_3:-86457699 Msa1133230:CDS
TATGCAGCAAGGACTCCTTA+TGG 0.473458 7_3:-86457865 Msa1133230:CDS
TTTGTCAATGGCTAAACCAT+TGG 0.477577 7_3:-86457938 Msa1133230:CDS
ATCCATGCATTCCTTACTAC+AGG 0.480935 7_3:-86457572 Msa1133230:three_prime_UTR
TATCTTTCTGGCTGTTGGAA+GGG 0.484079 7_3:-86459736 Msa1133230:CDS
GAAGAAAGGTGCTGATGCAT+TGG 0.489379 7_3:-86459382 Msa1133230:CDS
GGTGGTAGAAACAAGCGGAC+TGG 0.489930 7_3:-86457841 Msa1133230:CDS
AAAGGAAATGATTACTATGG+TGG 0.493627 7_3:-86457769 Msa1133230:CDS
GTGCACCGGATAGGAAGAAC+AGG 0.496326 7_3:-86458009 Msa1133230:CDS
GCATAACGGGTGAGCCATGC+AGG 0.503294 7_3:+86457883 None:intergenic
TTGTTTCTACCACCACCATA+AGG 0.503855 7_3:+86457850 None:intergenic
ACATAATCATCAATGTCATT+GGG 0.504262 7_3:+86458030 None:intergenic
GTTTGAAATTCTGTAGAAGA+GGG 0.512689 7_3:-86457496 Msa1133230:three_prime_UTR
TCTGCAGTGCATTCAAATGA+AGG 0.522655 7_3:+86459787 None:intergenic
GATGATTATGTGCACCGGAT+AGG 0.527907 7_3:-86458018 Msa1133230:CDS
CAGCAAAAGCAAACCAGTTG+TGG 0.528401 7_3:+86457532 None:intergenic
TTCCTGTAGTAAGGAATGCA+TGG 0.531327 7_3:+86457570 None:intergenic
TCAAGGTGTAACAAGTGCTT+GGG 0.532427 7_3:-86457698 Msa1133230:CDS
AAGGAGTCCTTGCTGCATAA+CGG 0.532448 7_3:+86457869 None:intergenic
CACGTGGTCTTGATATTCCA+CGG 0.535762 7_3:-86458080 Msa1133230:CDS
GTTCCTGCCAGTTATGGCGG+AGG 0.540492 7_3:-86457727 Msa1133230:CDS
GTGTTTGTGGAAACGAAGAA+AGG 0.546167 7_3:-86459396 Msa1133230:CDS
TACCCTCCGCCATAACTGGC+AGG 0.546471 7_3:+86457724 None:intergenic
AATGATTACTATGGTGGAAA+CGG 0.551471 7_3:-86457763 Msa1133230:CDS
TTTATGTTGTGGGTAGTATG+TGG 0.553930 7_3:-86457423 Msa1133230:three_prime_UTR
TGGTGGCCGTGATGTTAGGA+AGG 0.558530 7_3:-86457806 Msa1133230:CDS
CACTCCAATTCTAGTGGCAA+CGG 0.558952 7_3:-86458112 Msa1133230:CDS
TGACAAGAGAAGCCACCTCA+TGG 0.560777 7_3:-86459661 Msa1133230:CDS
GAAATTCTGTAGAAGAGGGA+GGG 0.562680 7_3:-86457492 Msa1133230:three_prime_UTR
GCAAGGACTCCTTATGGTGG+TGG 0.564052 7_3:-86457859 Msa1133230:CDS
ATAGGAAGAACAGGACGAGC+TGG 0.564587 7_3:-86458000 Msa1133230:CDS
TGAAATTCTGTAGAAGAGGG+AGG 0.573097 7_3:-86457493 Msa1133230:three_prime_UTR
GCATTGAGATCATTCAAGAC+TGG 0.575269 7_3:-86458138 Msa1133230:CDS
TACTCTTTGAGCAATTAGAT+CGG 0.575437 7_3:+86459701 None:intergenic
GGTTTGAAATTCTGTAGAAG+AGG 0.578896 7_3:-86457497 Msa1133230:three_prime_UTR
TACTATGGTGGAAACGGTGG+TGG 0.589805 7_3:-86457757 Msa1133230:CDS
GGTGTTCCTGCCAGTTATGG+CGG 0.592814 7_3:-86457730 Msa1133230:CDS
TTTCTCTCTGAGCATGAAGG+AGG 0.593891 7_3:+86459637 None:intergenic
GGCTCACCCGTTATGCAGCA+AGG 0.594047 7_3:-86457876 Msa1133230:CDS
CAGAGAGAAAATGATGTGAA+TGG 0.597153 7_3:-86459624 Msa1133230:CDS
GGTAGAAACAAGCGGACTGG+GGG 0.597859 7_3:-86457838 Msa1133230:CDS
TTCCTGCCAGTTATGGCGGA+GGG 0.597902 7_3:-86457726 Msa1133230:CDS
GCAACGGATGTTGCAGCACG+TGG 0.601102 7_3:-86458096 Msa1133230:CDS
TCAAAGAGTAGAATATGTGC+TGG 0.606920 7_3:-86459688 Msa1133230:CDS
AGAAAATGATGTGAATGGCA+AGG 0.613108 7_3:-86459619 Msa1133230:intron
ACGTGGTCTTGATATTCCAC+GGG 0.617920 7_3:-86458079 Msa1133230:CDS
TGGTAGAAACAAGCGGACTG+GGG 0.618436 7_3:-86457839 Msa1133230:CDS
CTGCAGTGCATTCAAATGAA+GGG 0.622343 7_3:+86459788 None:intergenic
TCTTGCATCAGATCAGCCAA+TGG 0.629103 7_3:+86457922 None:intergenic
AGCTCTCCTTCCTAACATCA+CGG 0.629638 7_3:+86457800 None:intergenic
TATGGCGGAGGGTATGGTCA+AGG 0.633849 7_3:-86457715 Msa1133230:CDS
TATTCCACGGGTAGCACATG+TGG 0.638046 7_3:-86458067 Msa1133230:CDS
ATTGGCTGATCTGATGCAAG+AGG 0.641218 7_3:-86457920 Msa1133230:CDS
GCATGGATTTAGGAAAACCA+TGG 0.643218 7_3:+86457587 None:intergenic
CATGAAGGAGGTCCATGAGG+TGG 0.651399 7_3:+86459649 None:intergenic
ACATTGATGATTATGTGCAC+CGG 0.653916 7_3:-86458023 Msa1133230:CDS
CAAATCAAGAAGTACCTGCA+TGG 0.655342 7_3:-86457897 Msa1133230:CDS
TGGAAACACTCCAATTCTAG+TGG 0.656487 7_3:-86458118 Msa1133230:CDS
GAGCATGAAGGAGGTCCATG+AGG 0.681402 7_3:+86459646 None:intergenic
TTTACCACATGTGCTACCCG+TGG 0.688558 7_3:+86458063 None:intergenic
GCAGCAAGGACTCCTTATGG+TGG 0.693000 7_3:-86457862 Msa1133230:CDS
GATTACTATGGTGGAAACGG+TGG 0.697976 7_3:-86457760 Msa1133230:CDS
ATGGTGGTGGTAGAAACAAG+CGG 0.731166 7_3:-86457846 Msa1133230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGATTTTTAAATTCTTA+AGG - chr7_3:86458644-86458663 Msa1133230:intron 10.0%
!! TGATAGAATTTAATAACTAA+TGG + chr7_3:86458940-86458959 None:intergenic 15.0%
!! GTAATTATTAATTAAGTGTA+GGG + chr7_3:86459915-86459934 None:intergenic 15.0%
!! TCTAATTAATTAACAGAGTT+TGG - chr7_3:86457320-86457339 Msa1133230:three_prime_UTR 20.0%
!! AATATCAGATGTTAGTAAAA+AGG + chr7_3:86458033-86458052 None:intergenic 20.0%
!! ACATCATTTAAATTTAATGG+AGG + chr7_3:86458279-86458298 None:intergenic 20.0%
!! GACACATCATTTAAATTTAA+TGG + chr7_3:86458282-86458301 None:intergenic 20.0%
!! AATAAAGGAAATGATTACTA+TGG - chr7_3:86459444-86459463 Msa1133230:intron 20.0%
!! GGTAATTATTAATTAAGTGT+AGG + chr7_3:86459916-86459935 None:intergenic 20.0%
! ATTCAAACTTGTACATATGT+TGG - chr7_3:86457647-86457666 Msa1133230:three_prime_UTR 25.0%
! CACTAACAAAATGTAAACTA+CGG + chr7_3:86457673-86457692 None:intergenic 25.0%
!!! CTCAATTAAGGATTTTTTTG+TGG - chr7_3:86457773-86457792 Msa1133230:CDS 25.0%
!! ATGATGTGTTCTATTTTCAT+TGG + chr7_3:86458139-86458158 None:intergenic 25.0%
!!! TGTTCAACTATTTTTTCAAG+CGG - chr7_3:86458220-86458239 Msa1133230:intron 25.0%
!! AAAACAAAAGAAGCTTCTTT+TGG + chr7_3:86458496-86458515 None:intergenic 25.0%
! AAATTGAAATTTCGAAGCAT+CGG + chr7_3:86458783-86458802 None:intergenic 25.0%
!!! AATTTGCGTTTTTTCTGTTT+TGG - chr7_3:86458798-86458817 Msa1133230:intron 25.0%
!!! ATTTGCGTTTTTTCTGTTTT+GGG - chr7_3:86458799-86458818 Msa1133230:intron 25.0%
! ATTCAATTCCAACAAAACAA+TGG + chr7_3:86458874-86458893 None:intergenic 25.0%
! TGAGAGAATTTAAGTACTAA+TGG + chr7_3:86458977-86458996 None:intergenic 25.0%
! AAAAGTCCTAACACAATATT+GGG + chr7_3:86459006-86459025 None:intergenic 25.0%
! ACATAATCATCAATGTCATT+GGG + chr7_3:86459189-86459208 None:intergenic 25.0%
!!! ATTTTACTGGTTTATGTTGT+GGG - chr7_3:86459783-86459802 Msa1133230:CDS 25.0%
! TAATATGTGCATGCTAATTA+GGG + chr7_3:86459864-86459883 None:intergenic 25.0%
! TTAATATGTGCATGCTAATT+AGG + chr7_3:86459865-86459884 None:intergenic 25.0%
TCTTGCTAGTTATATCTTTC+TGG - chr7_3:86457468-86457487 Msa1133230:three_prime_UTR 30.0%
TACTCTTTGAGCAATTAGAT+CGG + chr7_3:86457518-86457537 None:intergenic 30.0%
TTTAAGAGTAGGAAGATTTG+TGG - chr7_3:86457740-86457759 Msa1133230:CDS 30.0%
! ATGTAGAACTTAAAAGCACA+AGG + chr7_3:86458057-86458076 None:intergenic 30.0%
TAAGTTCTACATTCTCAATG+AGG - chr7_3:86458065-86458084 Msa1133230:CDS 30.0%
ATTGGTTGAGACTAAATATG+AGG + chr7_3:86458310-86458329 None:intergenic 30.0%
! ATGATGTGTCCTATTTTCAT+TGG - chr7_3:86458292-86458311 Msa1133230:intron 30.0%
TAGTCTCAACCAATGAAAAT+AGG + chr7_3:86458127-86458146 None:intergenic 30.0%
ATTGGTTGAGACTAAATATG+AGG - chr7_3:86458310-86458329 Msa1133230:intron 30.0%
!! AAAAGAAGCTTCTTTTGGAA+GGG + chr7_3:86458491-86458510 None:intergenic 30.0%
TTCACTATAGTTGAAATGAC+AGG - chr7_3:86458514-86458533 Msa1133230:intron 30.0%
GAAAAAAAATCCTCCAAAGT+AGG + chr7_3:86458584-86458603 None:intergenic 30.0%
ATGCGCTAAGAAATTTCAAT+AGG + chr7_3:86458702-86458721 None:intergenic 30.0%
ATTGAAATTTCTTAGCGCAT+CGG - chr7_3:86458702-86458721 Msa1133230:intron 30.0%
!!! TTTTTAGTTGTTGTGAACCA+TGG - chr7_3:86458836-86458855 Msa1133230:intron 30.0%
AATGGAACATCATAATACCA+TGG + chr7_3:86458856-86458875 None:intergenic 30.0%
!! ATGATGTTCCATTGTTTTGT+TGG - chr7_3:86458863-86458882 Msa1133230:intron 30.0%
GTTGGAATTGAATTTCTCTA+TGG - chr7_3:86458881-86458900 Msa1133230:intron 30.0%
CAAAAGTCCTAACACAATAT+TGG + chr7_3:86459007-86459026 None:intergenic 30.0%
AATGTAATTGTTCTGTTTGC+AGG - chr7_3:86459044-86459063 Msa1133230:intron 30.0%
CACATAATCATCAATGTCAT+TGG + chr7_3:86459190-86459209 None:intergenic 30.0%
CAAATTACTGTCATTGAAGA+AGG + chr7_3:86459262-86459281 None:intergenic 30.0%
! CAATGACAGTAATTTGTCAA+TGG - chr7_3:86459266-86459285 Msa1133230:intron 30.0%
AAGGAGAGCTCATATAATAA+AGG - chr7_3:86459429-86459448 Msa1133230:intron 30.0%
AAAGGAAATGATTACTATGG+TGG - chr7_3:86459447-86459466 Msa1133230:intron 30.0%
! AATGATTACTATGGTGGAAA+CGG - chr7_3:86459453-86459472 Msa1133230:intron 30.0%
! AAGTTGTATTTTCTCAACCA+TGG - chr7_3:86459612-86459631 Msa1133230:intron 30.0%
! GTTTGAAATTCTGTAGAAGA+GGG - chr7_3:86459720-86459739 Msa1133230:CDS 30.0%
! GGGTGTAAACTATATTGTTT+TGG - chr7_3:86459744-86459763 Msa1133230:CDS 30.0%
!!! TATATTGTTTTGGAGACTCA+AGG - chr7_3:86459754-86459773 Msa1133230:CDS 30.0%
!!! ATATTGTTTTGGAGACTCAA+GGG - chr7_3:86459755-86459774 Msa1133230:CDS 30.0%
!!! GATTTTACTGGTTTATGTTG+TGG - chr7_3:86459782-86459801 Msa1133230:CDS 30.0%
!! TGTGGTGTTTTGAATTTGAT+AGG - chr7_3:86459811-86459830 Msa1133230:CDS 30.0%
!! GTGGTGTTTTGAATTTGATA+GGG - chr7_3:86459812-86459831 Msa1133230:CDS 30.0%
!! AGTTATATCTTTCTGGCTGT+TGG - chr7_3:86457475-86457494 Msa1133230:three_prime_UTR 35.0%
TCAAAGAGTAGAATATGTGC+TGG - chr7_3:86457528-86457547 Msa1133230:three_prime_UTR 35.0%
CAGAGAGAAAATGATGTGAA+TGG - chr7_3:86457592-86457611 Msa1133230:three_prime_UTR 35.0%
AGAAAATGATGTGAATGGCA+AGG - chr7_3:86457597-86457616 Msa1133230:three_prime_UTR 35.0%
ATTCAAAAACTATGCTGCAC+TGG - chr7_3:86457705-86457724 Msa1133230:CDS 35.0%
ACAGTCGTGTATTTAAGAGT+AGG - chr7_3:86457729-86457748 Msa1133230:CDS 35.0%
!! GGTTTTGTCACACTCAATTA+AGG - chr7_3:86457761-86457780 Msa1133230:CDS 35.0%
! TTTTTGTGGTTTTTGCAGCA+AGG - chr7_3:86457787-86457806 Msa1133230:CDS 35.0%
AATGAGGAGAAATTCTTGCA+TGG - chr7_3:86458081-86458100 Msa1133230:CDS 35.0%
! GAGACTAAATATGAGGTGTT+AGG + chr7_3:86458317-86458336 None:intergenic 35.0%
TCAGTTGCATATCTTGGTAA+TGG + chr7_3:86458249-86458268 None:intergenic 35.0%
! GAGACTAAATATGAGGTGTT+AGG - chr7_3:86458317-86458336 Msa1133230:intron 35.0%
ATTGAGGAGAAATTCTTGCA+TGG + chr7_3:86458350-86458369 None:intergenic 35.0%
TCTCCTCAATGAGACTTTAA+TGG - chr7_3:86458360-86458379 Msa1133230:intron 35.0%
CTCCTCAATGAGACTTTAAT+GGG - chr7_3:86458361-86458380 Msa1133230:intron 35.0%
TCCTCAATGAGACTTTAATG+GGG - chr7_3:86458362-86458381 Msa1133230:intron 35.0%
AGTTTATACTGCCACAGAAA+TGG - chr7_3:86458437-86458456 Msa1133230:intron 35.0%
GTTTATACTGCCACAGAAAT+GGG - chr7_3:86458438-86458457 Msa1133230:intron 35.0%
!! AAAGAAGCTTCTTTTGGAAG+GGG + chr7_3:86458490-86458509 None:intergenic 35.0%
! CAAAAGAAGCTTCTTTTGGA+AGG + chr7_3:86458492-86458511 None:intergenic 35.0%
! GATGGACTTTCATGGTAATT+TGG + chr7_3:86458542-86458561 None:intergenic 35.0%
AAAATCCTCCAAAGTAGGAA+GGG + chr7_3:86458579-86458598 None:intergenic 35.0%
AAAAATCCTCCAAAGTAGGA+AGG + chr7_3:86458580-86458599 None:intergenic 35.0%
!!! TTTTTTCTGTTTTGGGTGCA+TGG - chr7_3:86458806-86458825 Msa1133230:intron 35.0%
AGCTTTCCCAATATTGTGTT+AGG - chr7_3:86458997-86459016 Msa1133230:intron 35.0%
! ACATTGATGATTATGTGCAC+CGG - chr7_3:86459193-86459212 Msa1133230:intron 35.0%
TTTGTCAATGGCTAAACCAT+TGG - chr7_3:86459278-86459297 Msa1133230:intron 35.0%
TCGCTAATTCATTCTCATGT+TGG - chr7_3:86459558-86459577 Msa1133230:intron 35.0%
CGCTAATTCATTCTCATGTT+GGG - chr7_3:86459559-86459578 Msa1133230:intron 35.0%
AGTAAGGAATGCATGGATTT+AGG + chr7_3:86459642-86459661 None:intergenic 35.0%
GACAAAGATTTCCTGTAGTA+AGG + chr7_3:86459658-86459677 None:intergenic 35.0%
! GGTTTGAAATTCTGTAGAAG+AGG - chr7_3:86459719-86459738 Msa1133230:CDS 35.0%
!!! TATTGTTTTGGAGACTCAAG+GGG - chr7_3:86459756-86459775 Msa1133230:CDS 35.0%
TTTATGTTGTGGGTAGTATG+TGG - chr7_3:86459793-86459812 Msa1133230:CDS 35.0%
CTGCAGTGCATTCAAATGAA+GGG + chr7_3:86457431-86457450 None:intergenic 40.0%
TCTGCAGTGCATTCAAATGA+AGG + chr7_3:86457432-86457451 None:intergenic 40.0%
!! ATATCTTTCTGGCTGTTGGA+AGG - chr7_3:86457479-86457498 Msa1133230:three_prime_UTR 40.0%
!! TATCTTTCTGGCTGTTGGAA+GGG - chr7_3:86457480-86457499 Msa1133230:three_prime_UTR 40.0%
! CATTTTCTCTCTGAGCATGA+AGG + chr7_3:86457585-86457604 None:intergenic 40.0%
AGGTCTGACTTTAGTGTTTG+TGG - chr7_3:86457807-86457826 Msa1133230:CDS 40.0%
GTGTTTGTGGAAACGAAGAA+AGG - chr7_3:86457820-86457839 Msa1133230:CDS 40.0%
GAACACTGCTTGTGTGTTAA+CGG - chr7_3:86457856-86457875 Msa1133230:CDS 40.0%
AACACTGCTTGTGTGTTAAC+GGG - chr7_3:86457857-86457876 Msa1133230:CDS 40.0%
TTTCCTGCAACTTGCATTCA+TGG - chr7_3:86457880-86457899 Msa1133230:CDS 40.0%
GCACTTTCAGTTGCATATCT+TGG + chr7_3:86458255-86458274 None:intergenic 40.0%
ACCCCATTAAAGTCTCATTG+AGG + chr7_3:86458366-86458385 None:intergenic 40.0%
AAAGCCAAATCCCATTTCTG+TGG + chr7_3:86458451-86458470 None:intergenic 40.0%
TTTGGAAGGGGGGAAAAATT+AGG + chr7_3:86458478-86458497 None:intergenic 40.0%
!! AAGAAGCTTCTTTTGGAAGG+GGG + chr7_3:86458489-86458508 None:intergenic 40.0%
! GTGAAAGAGATGGACTTTCA+TGG + chr7_3:86458550-86458569 None:intergenic 40.0%
!! GCATCGGTTCTATGGTTTAA+TGG + chr7_3:86458767-86458786 None:intergenic 40.0%
!! ATTTCGAAGCATCGGTTCTA+TGG + chr7_3:86458775-86458794 None:intergenic 40.0%
GCATTGAGATCATTCAAGAC+TGG - chr7_3:86459078-86459097 Msa1133230:intron 40.0%
TGGAAACACTCCAATTCTAG+TGG - chr7_3:86459098-86459117 Msa1133230:intron 40.0%
!! CAAATCAAGAAGTACCTGCA+TGG - chr7_3:86459319-86459338 Msa1133230:intron 40.0%
!! TTGTTTCTACCACCACCATA+AGG + chr7_3:86459369-86459388 None:intergenic 40.0%
TCAAGGTGTAACAAGTGCTT+GGG - chr7_3:86459518-86459537 Msa1133230:intron 40.0%
TGTAACAAGTGCTTGGGATT+AGG - chr7_3:86459524-86459543 Msa1133230:intron 40.0%
GCATGGATTTAGGAAAACCA+TGG + chr7_3:86459632-86459651 None:intergenic 40.0%
ATCCATGCATTCCTTACTAC+AGG - chr7_3:86459644-86459663 Msa1133230:CDS 40.0%
TTCCTGTAGTAAGGAATGCA+TGG + chr7_3:86459649-86459668 None:intergenic 40.0%
CTTTGTCAATGATCCACAAC+TGG - chr7_3:86459671-86459690 Msa1133230:CDS 40.0%
TGAAATTCTGTAGAAGAGGG+AGG - chr7_3:86459723-86459742 Msa1133230:CDS 40.0%
GAAATTCTGTAGAAGAGGGA+GGG - chr7_3:86459724-86459743 Msa1133230:CDS 40.0%
!!! ATTGTTTTGGAGACTCAAGG+GGG - chr7_3:86459757-86459776 Msa1133230:CDS 40.0%
!! AGGGTTCTTAGCTTTTGACT+CGG - chr7_3:86459831-86459850 Msa1133230:exon 40.0%
TTTCTCTCTGAGCATGAAGG+AGG + chr7_3:86457582-86457601 None:intergenic 45.0%
TGAATAGCAGCAGTATGCAG+AGG + chr7_3:86457632-86457651 None:intergenic 45.0%
!! GAAGAAAGGTGCTGATGCAT+TGG - chr7_3:86457834-86457853 Msa1133230:CDS 45.0%
ACTGCCACAGAAATGGGATT+TGG - chr7_3:86458444-86458463 Msa1133230:intron 45.0%
!! AGAAGCTTCTTTTGGAAGGG+GGG + chr7_3:86458488-86458507 None:intergenic 45.0%
AGGGAACATGGTGAAAGAGA+TGG + chr7_3:86458560-86458579 None:intergenic 45.0%
ACCATGTTCCCTTCCTACTT+TGG - chr7_3:86458568-86458587 Msa1133230:intron 45.0%
TCCAAAGTAGGAAGGGAACA+TGG + chr7_3:86458572-86458591 None:intergenic 45.0%
ATGTTCCCTTCCTACTTTGG+AGG - chr7_3:86458571-86458590 Msa1133230:intron 45.0%
TCTTAGCGCATCGGATATGT+AGG - chr7_3:86458711-86458730 Msa1133230:intron 45.0%
!! TTTCTGTTTTGGGTGCATGG+TGG - chr7_3:86458809-86458828 Msa1133230:intron 45.0%
CACTCCAATTCTAGTGGCAA+CGG - chr7_3:86459104-86459123 Msa1133230:intron 45.0%
ACATCCGTTGCCACTAGAAT+TGG + chr7_3:86459111-86459130 None:intergenic 45.0%
! CACGTGGTCTTGATATTCCA+CGG - chr7_3:86459136-86459155 Msa1133230:intron 45.0%
! ACGTGGTCTTGATATTCCAC+GGG - chr7_3:86459137-86459156 Msa1133230:intron 45.0%
GATGATTATGTGCACCGGAT+AGG - chr7_3:86459198-86459217 Msa1133230:intron 45.0%
AACAGGACGAGCTGGTAAAA+TGG - chr7_3:86459224-86459243 Msa1133230:intron 45.0%
ACAGGACGAGCTGGTAAAAT+GGG - chr7_3:86459225-86459244 Msa1133230:intron 45.0%
TCTTGCATCAGATCAGCCAA+TGG + chr7_3:86459297-86459316 None:intergenic 45.0%
ATTGGCTGATCTGATGCAAG+AGG - chr7_3:86459296-86459315 Msa1133230:intron 45.0%
AAGGAGTCCTTGCTGCATAA+CGG + chr7_3:86459350-86459369 None:intergenic 45.0%
TATGCAGCAAGGACTCCTTA+TGG - chr7_3:86459351-86459370 Msa1133230:CDS 45.0%
!! ATGGTGGTGGTAGAAACAAG+CGG - chr7_3:86459370-86459389 Msa1133230:CDS 45.0%
TAACATCACGGCCACCAAAA+CGG + chr7_3:86459407-86459426 None:intergenic 45.0%
AGCTCTCCTTCCTAACATCA+CGG + chr7_3:86459419-86459438 None:intergenic 45.0%
! GATTACTATGGTGGAAACGG+TGG - chr7_3:86459456-86459475 Msa1133230:intron 45.0%
TATGGTGTTCCTGCCAGTTA+TGG - chr7_3:86459483-86459502 Msa1133230:intron 45.0%
GTCAAGGTGTAACAAGTGCT+TGG - chr7_3:86459517-86459536 Msa1133230:intron 45.0%
CAGCAAAAGCAAACCAGTTG+TGG + chr7_3:86459687-86459706 None:intergenic 45.0%
!!! CACAACTGGTTTGCTTTTGC+TGG - chr7_3:86459685-86459704 Msa1133230:CDS 45.0%
!!! CTGGTTTGCTTTTGCTGGTT+AGG - chr7_3:86459690-86459709 Msa1133230:CDS 45.0%
!! CTTTTGCTGGTTAGGCACAT+AGG - chr7_3:86459698-86459717 Msa1133230:CDS 45.0%
!! GGGTTCTTAGCTTTTGACTC+GGG - chr7_3:86459832-86459851 Msa1133230:exon 45.0%
!! TTTCTGGCTGTTGGAAGGGT+TGG - chr7_3:86457484-86457503 Msa1133230:three_prime_UTR 50.0%
TGACAAGAGAAGCCACCTCA+TGG - chr7_3:86457555-86457574 Msa1133230:three_prime_UTR 50.0%
TCGCCATGAATGCAAGTTGC+AGG + chr7_3:86457886-86457905 None:intergenic 50.0%
TATTCCACGGGTAGCACATG+TGG - chr7_3:86459149-86459168 Msa1133230:intron 50.0%
TTTACCACATGTGCTACCCG+TGG + chr7_3:86459156-86459175 None:intergenic 50.0%
CTCGTCCTGTTCTTCCTATC+CGG + chr7_3:86459215-86459234 None:intergenic 50.0%
ATAGGAAGAACAGGACGAGC+TGG - chr7_3:86459216-86459235 Msa1133230:intron 50.0%
AGGAGTCCTTGCTGCATAAC+GGG + chr7_3:86459349-86459368 None:intergenic 50.0%
! GTGGTAGAAACAAGCGGACT+GGG - chr7_3:86459376-86459395 Msa1133230:CDS 50.0%
!! TGGTAGAAACAAGCGGACTG+GGG - chr7_3:86459377-86459396 Msa1133230:CDS 50.0%
AACATCACGGCCACCAAAAC+GGG + chr7_3:86459406-86459425 None:intergenic 50.0%
!! GTTTTGGTGGCCGTGATGTT+AGG - chr7_3:86459406-86459425 Msa1133230:CDS 50.0%
! TACTATGGTGGAAACGGTGG+TGG - chr7_3:86459459-86459478 Msa1133230:intron 50.0%
! CTCAAGGGGGCAGATTTTAC+TGG - chr7_3:86459770-86459789 Msa1133230:CDS 50.0%
CATGAAGGAGGTCCATGAGG+TGG + chr7_3:86457570-86457589 None:intergenic 55.0%
GAGCATGAAGGAGGTCCATG+AGG + chr7_3:86457573-86457592 None:intergenic 55.0%
GTGCACCGGATAGGAAGAAC+AGG - chr7_3:86459207-86459226 Msa1133230:intron 55.0%
GCAGCAAGGACTCCTTATGG+TGG - chr7_3:86459354-86459373 Msa1133230:CDS 55.0%
GCAAGGACTCCTTATGGTGG+TGG - chr7_3:86459357-86459376 Msa1133230:CDS 55.0%
GGTGGTAGAAACAAGCGGAC+TGG - chr7_3:86459375-86459394 Msa1133230:CDS 55.0%
!! GGTAGAAACAAGCGGACTGG+GGG - chr7_3:86459378-86459397 Msa1133230:CDS 55.0%
TGGTGGCCGTGATGTTAGGA+AGG - chr7_3:86459410-86459429 Msa1133230:CDS 55.0%
! GGTGGAAACGGTGGTGGTTA+TGG - chr7_3:86459465-86459484 Msa1133230:intron 55.0%
GGTGTTCCTGCCAGTTATGG+CGG - chr7_3:86459486-86459505 Msa1133230:intron 55.0%
! TTCCTGCCAGTTATGGCGGA+GGG - chr7_3:86459490-86459509 Msa1133230:intron 55.0%
ACCATACCCTCCGCCATAAC+TGG + chr7_3:86459499-86459518 None:intergenic 55.0%
! TATGGCGGAGGGTATGGTCA+AGG - chr7_3:86459501-86459520 Msa1133230:intron 55.0%
GCAACGGATGTTGCAGCACG+TGG - chr7_3:86459120-86459139 Msa1133230:intron 60.0%
!! GCATAACGGGTGAGCCATGC+AGG + chr7_3:86459336-86459355 None:intergenic 60.0%
GGCTCACCCGTTATGCAGCA+AGG - chr7_3:86459340-86459359 Msa1133230:CDS 60.0%
GTTCCTGCCAGTTATGGCGG+AGG - chr7_3:86459489-86459508 Msa1133230:intron 60.0%
TACCCTCCGCCATAACTGGC+AGG + chr7_3:86459495-86459514 None:intergenic 60.0%
! GCCAGTTATGGCGGAGGGTA+TGG - chr7_3:86459495-86459514 Msa1133230:intron 60.0%
! ACTGGGGGAGCCCGTTTTGG+TGG - chr7_3:86459393-86459412 Msa1133230:CDS 65.0%
!! CGGACTGGGGGAGCCCGTTT+TGG - chr7_3:86459390-86459409 Msa1133230:CDS 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 86457283 86459955 86457283 ID=Msa1133230;Name=Msa1133230
chr7_3 mRNA 86457283 86459955 86457283 ID=Msa1133230-mRNA-1;Parent=Msa1133230;Name=Msa1133230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=117|1|1|1|1|1|3|410|270
chr7_3 exon 86457283 86458172 86457283 ID=Msa1133230-mRNA-1:exon:25998;Parent=Msa1133230-mRNA-1
chr7_3 exon 86459320 86459433 86459320 ID=Msa1133230-mRNA-1:exon:25997;Parent=Msa1133230-mRNA-1
chr7_3 exon 86459620 86459955 86459620 ID=Msa1133230-mRNA-1:exon:25996;Parent=Msa1133230-mRNA-1
chr7_3 five_prime_UTR 86459839 86459955 86459839 ID=Msa1133230-mRNA-1:five_prime_utr;Parent=Msa1133230-mRNA-1
chr7_3 CDS 86459620 86459838 86459620 ID=Msa1133230-mRNA-1:cds;Parent=Msa1133230-mRNA-1
chr7_3 CDS 86459320 86459433 86459320 ID=Msa1133230-mRNA-1:cds;Parent=Msa1133230-mRNA-1
chr7_3 CDS 86457693 86458172 86457693 ID=Msa1133230-mRNA-1:cds;Parent=Msa1133230-mRNA-1
chr7_3 three_prime_UTR 86457283 86457692 86457283 ID=Msa1133230-mRNA-1:three_prime_utr;Parent=Msa1133230-mRNA-1
Gene Sequence

>Msa1133230

ATGATTGATATAATAATGATTATGATGTCCCTTCATTTGAATGCACTGCAGAGACTTGCTTCAGATTTTCTTGCTAGTTATATCTTTCTGGCTGTTGGAAGGGTTGGTTCAAGTACCGATCTAATTGCTCAAAGAGTAGAATATGTGCTGGAGTCTGACAAGAGAAGCCACCTCATGGACCTCCTTCATGCTCAGAGAGAAAATGATGTGAATGGCAAGCAAGGTCTGACTTTAGTGTTTGTGGAAACGAAGAAAGGTGCTGATGCATTGGAACACTGCTTGTGTGTTAACGGGTTTCCTGCAACTTGCATTCATGGCGACAGAACACAGCAAGAAAGAGAACAAGCATTGAGATCATTCAAGACTGGAAACACTCCAATTCTAGTGGCAACGGATGTTGCAGCACGTGGTCTTGATATTCCACGGGTAGCACATGTGGTAAACTTTGATCTTCCCAATGACATTGATGATTATGTGCACCGGATAGGAAGAACAGGACGAGCTGGTAAAATGGGATTAGCAACAGCCTTCTTCAATGACAGTAATTTGTCAATGGCTAAACCATTGGCTGATCTGATGCAAGAGGCAAATCAAGAAGTACCTGCATGGCTCACCCGTTATGCAGCAAGGACTCCTTATGGTGGTGGTAGAAACAAGCGGACTGGGGGAGCCCGTTTTGGTGGCCGTGATGTTAGGAAGGAGAGCTCATATAATAAAGGAAATGATTACTATGGTGGAAACGGTGGTGGTTATGGTGTTCCTGCCAGTTATGGCGGAGGGTATGGTCAAGGTGTAACAAGTGCTTGGGATTAG
Protein sequence

>Msa1133230

MIDIIMIMMSLHLNALQRLASDFLASYIFLAVGRVGSSTDLIAQRVEYVLESDKRSHLMDLLHAQRENDVNGKQGLTLVFVETKKGADALEHCLCVNGFPATCIHGDRTQQEREQALRSFKTGNTPILVATDVAARGLDIPRVAHVVNFDLPNDIDDYVHRIGRTGRAGKMGLATAFFNDSNLSMAKPLADLMQEANQEVPAWLTRYAARTPYGGGRNKRTGGARFGGRDVRKESSYNKGNDYYGGNGGGYGVPASYGGGYGQGVTSAWD*