AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0580100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0580100 MtrunA17_Chr3g0111111 75.714 140 34 0 1 140 1 140 1.28e-71 211
Msa0580100 MtrunA17_Chr3g0111191 71.795 117 33 0 1 117 1 117 1.48e-55 169
Msa0580100 MtrunA17_Chr3g0111281 58.865 141 56 2 1 140 1 140 1.76e-50 157
Msa0580100 MtrunA17_Chr3g0111261 58.571 140 54 2 1 140 1 136 6.53e-47 148
Msa0580100 MtrunA17_Chr3g0111351 52.083 144 57 3 1 140 9 144 8.24e-44 140
Msa0580100 MtrunA17_Chr3g0111081 57.664 137 50 5 1 136 1 130 1.83e-42 137
Msa0580100 MtrunA17_Chr6g0464071 52.817 142 57 4 1 140 1 134 8.12e-42 135
Msa0580100 MtrunA17_Chr3g0111241 51.429 140 58 2 1 140 1 130 1.93e-40 132
Msa0580100 MtrunA17_Chr3g0111131 52.593 135 56 4 1 133 1 129 1.98e-40 131
Msa0580100 MtrunA17_Chr3g0111071 54.745 137 54 4 1 136 1 130 5.01e-40 130
Msa0580100 MtrunA17_Chr3g0111711 68.932 103 29 3 1 102 1 101 6.81e-39 127
Msa0580100 MtrunA17_Chr3g0104571 49.286 140 63 4 1 138 1 134 8.24e-35 117
Msa0580100 MtrunA17_Chr7g0236791 46.154 143 69 3 1 140 1 138 4.36e-33 113
Msa0580100 MtrunA17_Chr7g0236801 46.528 144 68 5 1 140 1 139 1.11e-32 112
Msa0580100 MtrunA17_Chr3g0111221 54.167 120 48 3 22 140 1 114 7.42e-31 107
Msa0580100 MtrunA17_Chr6g0456951 44.681 141 65 7 1 136 1 133 1.05e-29 104
Msa0580100 MtrunA17_Chr6g0468601 41.259 143 67 4 1 136 1 133 2.07e-29 103
Msa0580100 MtrunA17_Chr3g0111201 87.037 54 7 0 87 140 3 56 5.49e-29 100
Msa0580100 MtrunA17_Chr3g0111311 53.535 99 39 3 22 120 1 92 1.31e-28 100
Msa0580100 MtrunA17_Chr1g0153591 42.520 127 55 3 1 126 1 110 2.56e-27 97.8
Msa0580100 MtrunA17_Chr6g0468621 41.135 141 67 4 1 136 1 130 5.19e-27 97.8
Msa0580100 MtrunA17_Chr3g0090571 38.961 154 74 6 1 138 1 150 6.15e-27 98.2
Msa0580100 MtrunA17_Chr7g0232011 43.548 124 59 4 1 123 1 114 4.11e-24 90.9
Msa0580100 MtrunA17_Chr8g0359071 38.843 121 64 2 3 121 4 116 2.34e-23 88.6
Msa0580100 MtrunA17_Chr4g0012121 41.781 146 68 5 1 136 1 139 2.35e-23 89.0
Msa0580100 MtrunA17_Chr8g0344801 42.105 95 48 2 43 136 7 95 7.34e-20 78.6
Msa0580100 MtrunA17_Chr6g0456991 44.318 88 42 2 43 129 27 108 1.94e-19 77.8
Msa0580100 MtrunA17_Chr8g0344771 40.678 118 59 6 22 136 1 110 1.48e-18 75.9
Msa0580100 MtrunA17_Chr7g0231991 38.806 134 59 4 22 138 1 128 2.13e-18 75.9
Msa0580100 MtrunA17_Chr8g0344791 39.130 115 60 5 22 133 1 108 7.31e-18 73.9
Msa0580100 MtrunA17_Chr7g0225821 36.719 128 70 5 1 124 1 121 2.85e-17 72.8
Msa0580100 MtrunA17_Chr8g0357331 36.458 96 40 2 64 138 2 97 3.01e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAATTCTAATCTCAAATA+TGG 0.216722 4_1:-85144972 Msa0580100:CDS
CCATATTTGAGATTAGAATT+TGG 0.241765 4_1:+85144972 None:intergenic
TGTACGATCGGTTATTGTTA+TGG 0.354008 4_1:-85145119 Msa0580100:CDS
GTCTTCTTGCTTGCTGCATT+CGG 0.367498 4_1:-85145408 Msa0580100:intron
TTTGCTTCTACTTTCTTCAT+TGG 0.374147 4_1:+85145212 None:intergenic
CTTGTAAGCGTCTTGTGCTT+CGG 0.409202 4_1:+85144929 None:intergenic
GAAAATCGCAATGTGGTAAG+TGG 0.449479 4_1:-85145089 Msa0580100:CDS
TTGACATATCAATATTAAGC+AGG 0.480895 4_1:+85144858 None:intergenic
TTTCTTGAATTTATAGCTAG+AGG 0.515321 4_1:+85144902 None:intergenic
AGGAACATCCTAAGTTATGT+TGG 0.542665 4_1:-85145046 Msa0580100:CDS
TCATGAGACCAACATAACTT+AGG 0.547157 4_1:+85145038 None:intergenic
AAAGTAGAAGCAAATTGTGA+TGG 0.553230 4_1:-85145203 Msa0580100:CDS
AAACTCGTTGGTTGTACGAT+CGG 0.575852 4_1:-85145131 Msa0580100:CDS
AGCACAAGACGCTTACAAGA+TGG 0.577959 4_1:-85144925 Msa0580100:CDS
AAAATCGCAATGTGGTAAGT+GGG 0.583821 4_1:-85145088 Msa0580100:CDS
GGTTGTAAATGCAAACTCGT+TGG 0.593162 4_1:-85145143 Msa0580100:CDS
GTCTTGTGCTTCGGAGACAG+AGG 0.642879 4_1:+85144938 None:intergenic
TTTATGCTAGACTGTGATAG+CGG 0.659883 4_1:-85145164 Msa0580100:CDS
TACCTGCGAAAATCGCAATG+TGG 0.731640 4_1:-85145096 Msa0580100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTATTATTATATTAA+TGG - chr4_1:85145023-85145042 Msa0580100:CDS 0.0%
! AATGCATGAATGGTATATAT+TGG + chr4_1:85144952-85144971 None:intergenic 25.0%
! CATAAATAGAAATGCATGAA+TGG + chr4_1:85144962-85144981 None:intergenic 25.0%
!! CCATATTTGAGATTAGAATT+TGG + chr4_1:85145341-85145360 None:intergenic 25.0%
! CCAAATTCTAATCTCAAATA+TGG - chr4_1:85145338-85145357 Msa0580100:intron 25.0%
! TTTCTTGAATTTATAGCTAG+AGG + chr4_1:85145411-85145430 None:intergenic 25.0%
TTTGCTTCTACTTTCTTCAT+TGG + chr4_1:85145101-85145120 None:intergenic 30.0%
AAAGTAGAAGCAAATTGTGA+TGG - chr4_1:85145107-85145126 Msa0580100:CDS 30.0%
TCATGATGAATGCACAAAAA+AGG - chr4_1:85145289-85145308 Msa0580100:intron 30.0%
CATGATGAATGCACAAAAAA+GGG - chr4_1:85145290-85145309 Msa0580100:intron 30.0%
TTTATGCTAGACTGTGATAG+CGG - chr4_1:85145146-85145165 Msa0580100:CDS 35.0%
TGTACGATCGGTTATTGTTA+TGG - chr4_1:85145191-85145210 Msa0580100:CDS 35.0%
AAAATCGCAATGTGGTAAGT+GGG - chr4_1:85145222-85145241 Msa0580100:CDS 35.0%
! GAATTTTCAGAGGAAGGTTA+AGG - chr4_1:85145244-85145263 Msa0580100:intron 35.0%
AGGAACATCCTAAGTTATGT+TGG - chr4_1:85145264-85145283 Msa0580100:intron 35.0%
TCATGAGACCAACATAACTT+AGG + chr4_1:85145275-85145294 None:intergenic 35.0%
AATGCACAAAAAAGGGTAGT+GGG - chr4_1:85145297-85145316 Msa0580100:intron 35.0%
GGTTGTAAATGCAAACTCGT+TGG - chr4_1:85145167-85145186 Msa0580100:CDS 40.0%
! AAACTCGTTGGTTGTACGAT+CGG - chr4_1:85145179-85145198 Msa0580100:CDS 40.0%
GAAAATCGCAATGTGGTAAG+TGG - chr4_1:85145221-85145240 Msa0580100:CDS 40.0%
! TGGTAAGTGGGAATTTTCAG+AGG - chr4_1:85145234-85145253 Msa0580100:intron 40.0%
! AAGTGGGAATTTTCAGAGGA+AGG - chr4_1:85145238-85145257 Msa0580100:intron 40.0%
GAATGCACAAAAAAGGGTAG+TGG - chr4_1:85145296-85145315 Msa0580100:intron 40.0%
! GTCTTCTTGCTTGCTGCATT+CGG - chr4_1:85144902-85144921 Msa0580100:CDS 45.0%
TACCTGCGAAAATCGCAATG+TGG - chr4_1:85145214-85145233 Msa0580100:CDS 45.0%
! TACCACATTGCGATTTTCGC+AGG + chr4_1:85145219-85145238 None:intergenic 45.0%
CTTGTAAGCGTCTTGTGCTT+CGG + chr4_1:85145384-85145403 None:intergenic 45.0%
AGCACAAGACGCTTACAAGA+TGG - chr4_1:85145385-85145404 Msa0580100:intron 45.0%
GTCTTGTGCTTCGGAGACAG+AGG + chr4_1:85145375-85145394 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 85144872 85145460 85144872 ID=Msa0580100;Name=Msa0580100
chr4_1 mRNA 85144872 85145460 85144872 ID=Msa0580100-mRNA-1;Parent=Msa0580100;Name=Msa0580100-mRNA-1;_AED=0.19;_eAED=0.20;_QI=0|1|0|1|1|1|2|0|140
chr4_1 exon 85145409 85145460 85145409 ID=Msa0580100-mRNA-1:exon:31181;Parent=Msa0580100-mRNA-1
chr4_1 exon 85144872 85145242 85144872 ID=Msa0580100-mRNA-1:exon:31180;Parent=Msa0580100-mRNA-1
chr4_1 CDS 85145409 85145460 85145409 ID=Msa0580100-mRNA-1:cds;Parent=Msa0580100-mRNA-1
chr4_1 CDS 85144872 85145242 85144872 ID=Msa0580100-mRNA-1:cds;Parent=Msa0580100-mRNA-1
Gene Sequence

>Msa0580100

ATGGCTTATGTTAAGCTTGCTTCTTTTGCTGTCTTCTTGCTTGCTGCATTCGTGATATTACCAATGAAGAAAGTAGAAGCAAATTGTGATGGACGTCTTTGCGATAGCTTTATGCTAGACTGTGATAGCGGTTGTAAATGCAAACTCGTTGGTTGTACGATCGGTTATTGTTATGGTACCTGCGAAAATCGCAATGTGGTAAGTGGGAATTTTCAGAGGAAGGTTAAGGAACATCCTAAGTTATGTTGGTCTCATGATGAATGCACAAAAAAGGGTAGTGGGAATTATTGTGCTCGTTTTCCAAATTCTAATCTCAAATATGGCTTCTGTTTTGCCTCTGTCTCCGAAGCACAAGACGCTTACAAGATGGCCTCTAGCTATAAATTCAAGAAAGACTTCTTGAAGATGTCTGTTCCTGCTTAA

Protein sequence

>Msa0580100

MAYVKLASFAVFLLAAFVILPMKKVEANCDGRLCDSFMLDCDSGCKCKLVGCTIGYCYGTCENRNVVSGNFQRKVKEHPKLCWSHDECTKKGSGNYCARFPNSNLKYGFCFASVSEAQDAYKMASSYKFKKDFLKMSVPA*