Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1390170 | XP_003600698.1 | 92.265 | 181 | 14 | 0 | 1 | 181 | 1 | 181 | 4.15e-121 | 349 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1390170 | G7J8S2 | 92.265 | 181 | 14 | 0 | 1 | 181 | 1 | 181 | 1.98e-121 | 349 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1390170 | Msa1420060 | 0.821514 | 3.877683e-53 | -8.615850e-47 |
| Msa0203160 | Msa1390170 | 0.812060 | 5.125663e-51 | -8.615850e-47 |
| Msa0219190 | Msa1390170 | 0.804432 | 2.165912e-49 | -8.615850e-47 |
| Msa1007350 | Msa1390170 | 0.823167 | 1.602283e-53 | -8.615850e-47 |
| Msa1032450 | Msa1390170 | 0.810516 | 1.108761e-50 | -8.615850e-47 |
| Msa1041530 | Msa1390170 | 0.805498 | 1.296634e-49 | -8.615850e-47 |
| Msa0336090 | Msa1390170 | 0.808650 | 2.790065e-50 | -8.615850e-47 |
| Msa1127140 | Msa1390170 | 0.805140 | 1.540998e-49 | -8.615850e-47 |
| Msa1130010 | Msa1390170 | 0.800340 | 1.509180e-48 | -8.615850e-47 |
| Msa1136230 | Msa1390170 | 0.819115 | 1.375886e-52 | -8.615850e-47 |
| Msa1150970 | Msa1390170 | 0.803641 | 3.163792e-49 | -8.615850e-47 |
| Msa1166400 | Msa1390170 | 0.800865 | 1.179202e-48 | -8.615850e-47 |
| Msa0873190 | Msa1390170 | 0.804286 | 2.323706e-49 | -8.615850e-47 |
| Msa0905550 | Msa1390170 | 0.810551 | 1.089745e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1390170 | MtrunA17_Chr3g0110231 | 92.265 | 181 | 14 | 0 | 1 | 181 | 1 | 181 | 3.82e-125 | 349 |
| Msa1390170 | MtrunA17_Chr5g0434461 | 40.223 | 179 | 86 | 6 | 1 | 170 | 1 | 167 | 3.83e-25 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1390170 | AT5G37550.1 | 37.195 | 164 | 80 | 8 | 25 | 168 | 50 | 210 | 6.90e-16 | 73.2 |
Find 40 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTGGATGGAAGTGCTTTA+AGG | 0.237219 | tig0021332:-41126 | Msa1390170:CDS |
| TTATAAACTTCAAAGGGTTT+TGG | 0.325679 | tig0021332:-41186 | Msa1390170:CDS |
| TCAAGAAATGTTGTTGTTTG+TGG | 0.387907 | tig0021332:-41163 | Msa1390170:CDS |
| TGGCCAAGGTAACTGATCTT+AGG | 0.402243 | tig0021332:-41387 | Msa1390170:CDS |
| ATTGTCATCCATATGTTTGT+TGG | 0.412328 | tig0021332:+41258 | None:intergenic |
| TTTCTCTCTGAGCTCTCTCA+TGG | 0.436507 | tig0021332:-41593 | Msa1390170:CDS |
| TCTCTGCTTTCTTGAGGTTG+AGG | 0.440465 | tig0021332:+41556 | None:intergenic |
| CTCAAGTACCAACAAACATA+TGG | 0.441612 | tig0021332:-41266 | Msa1390170:CDS |
| AATGTTGTTGTTTGTGGTGG+TGG | 0.453933 | tig0021332:-41157 | Msa1390170:CDS |
| AGTGGATGGAAGTGCTTTAA+GGG | 0.453943 | tig0021332:-41125 | Msa1390170:CDS |
| AGAATGATGATGGTAATAGA+AGG | 0.459333 | tig0021332:-41228 | Msa1390170:CDS |
| AAAAGGTTTATAAACTTCAA+AGG | 0.467170 | tig0021332:-41193 | Msa1390170:CDS |
| AAAGGTTTATAAACTTCAAA+GGG | 0.469059 | tig0021332:-41192 | Msa1390170:CDS |
| AGCTTATCTCTGCTTTCTTG+AGG | 0.470855 | tig0021332:+41550 | None:intergenic |
| GAAGGATGAATAATAAGAAA+AGG | 0.487756 | tig0021332:-41210 | Msa1390170:CDS |
| ACGAAGTACAAAGCATTTCA+AGG | 0.503831 | tig0021332:-41309 | Msa1390170:CDS |
| CCTTGTAAAAGCAGCTGCAT+GGG | 0.506382 | tig0021332:-41524 | Msa1390170:CDS |
| CCCTTGTAAAAGCAGCTGCA+TGG | 0.515732 | tig0021332:-41525 | Msa1390170:CDS |
| AGAAATGTTGTTGTTTGTGG+TGG | 0.518716 | tig0021332:-41160 | Msa1390170:CDS |
| TTTCAGATCTTGAACCATGT+TGG | 0.535997 | tig0021332:+41494 | None:intergenic |
| ATGTTGTTGTTTGTGGTGGT+GGG | 0.536424 | tig0021332:-41156 | Msa1390170:CDS |
| AGAAGCAATGCGAATGGCCA+AGG | 0.547792 | tig0021332:-41401 | Msa1390170:CDS |
| TAAAAGCAGCTGCATGGGCA+TGG | 0.548811 | tig0021332:-41519 | Msa1390170:CDS |
| AACTTGTAACGAGAAGGCCT+CGG | 0.561358 | tig0021332:+41424 | None:intergenic |
| CTTGCTAGATACTTAACTAG+AGG | 0.565930 | tig0021332:+41072 | None:intergenic |
| TCTTAGGTGCAACGGACTTG+AGG | 0.567071 | tig0021332:-41371 | Msa1390170:CDS |
| TGGTGGTGGGAATGATGTAG+TGG | 0.574409 | tig0021332:-41143 | Msa1390170:CDS |
| AGGTGCAACGGACTTGAGGT+TGG | 0.576584 | tig0021332:-41367 | Msa1390170:CDS |
| GGTGGGAATGATGTAGTGGA+TGG | 0.584519 | tig0021332:-41139 | Msa1390170:CDS |
| AATTGTGCAAAGAATGATGA+TGG | 0.588625 | tig0021332:-41238 | Msa1390170:CDS |
| GCTTCTAACTTGTAACGAGA+AGG | 0.592890 | tig0021332:+41418 | None:intergenic |
| GCACCTAAGATCAGTTACCT+TGG | 0.604459 | tig0021332:+41384 | None:intergenic |
| GTAACTGATCTTAGGTGCAA+CGG | 0.616466 | tig0021332:-41379 | Msa1390170:CDS |
| ATCAGTTGTCTGTGAAGCAT+AGG | 0.619879 | tig0021332:-41099 | Msa1390170:CDS |
| TGTTGGTACTTGAGTCAATG+AGG | 0.623112 | tig0021332:+41275 | None:intergenic |
| CAAGTTAGAAGCAATGCGAA+TGG | 0.644774 | tig0021332:-41407 | Msa1390170:CDS |
| GTTATATAATCATGTCACCA+AGG | 0.666182 | tig0021332:-41618 | None:intergenic |
| GCATGGGCATGGTACCAACA+TGG | 0.678579 | tig0021332:-41508 | Msa1390170:CDS |
| GTGGATGGAAGTGCTTTAAG+GGG | 0.679790 | tig0021332:-41124 | Msa1390170:CDS |
| CAAGAACTCGACGTTCACCG+AGG | 0.736761 | tig0021332:-41441 | Msa1390170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGGTTTATAAACTTCAA+AGG | - | tig0021332:41498-41517 | Msa1390170:CDS | 20.0% |
| !! | AAAGGTTTATAAACTTCAAA+GGG | - | tig0021332:41499-41518 | Msa1390170:CDS | 20.0% |
| ! | GAAGGATGAATAATAAGAAA+AGG | - | tig0021332:41481-41500 | Msa1390170:CDS | 25.0% |
| !! | TTATAAACTTCAAAGGGTTT+TGG | - | tig0021332:41505-41524 | Msa1390170:CDS | 25.0% |
| ATTGTCATCCATATGTTTGT+TGG | + | tig0021332:41436-41455 | None:intergenic | 30.0% | |
| AATTGTGCAAAGAATGATGA+TGG | - | tig0021332:41453-41472 | Msa1390170:CDS | 30.0% | |
| !! | AGAATGATGATGGTAATAGA+AGG | - | tig0021332:41463-41482 | Msa1390170:CDS | 30.0% |
| !! | TCAAGAAATGTTGTTGTTTG+TGG | - | tig0021332:41528-41547 | Msa1390170:CDS | 30.0% |
| TTTCAGATCTTGAACCATGT+TGG | + | tig0021332:41200-41219 | None:intergenic | 35.0% | |
| ! | ACGAAGTACAAAGCATTTCA+AGG | - | tig0021332:41382-41401 | Msa1390170:CDS | 35.0% |
| CTCAAGTACCAACAAACATA+TGG | - | tig0021332:41425-41444 | Msa1390170:CDS | 35.0% | |
| !! | AGAAATGTTGTTGTTTGTGG+TGG | - | tig0021332:41531-41550 | Msa1390170:CDS | 35.0% |
| GCTCTCTCATGGAGAAAAAA+AGG | - | tig0021332:41109-41128 | Msa1390170:CDS | 40.0% | |
| AGCTTATCTCTGCTTTCTTG+AGG | + | tig0021332:41144-41163 | None:intergenic | 40.0% | |
| ! | GCTTCTAACTTGTAACGAGA+AGG | + | tig0021332:41276-41295 | None:intergenic | 40.0% |
| CAAGTTAGAAGCAATGCGAA+TGG | - | tig0021332:41284-41303 | Msa1390170:CDS | 40.0% | |
| GTAACTGATCTTAGGTGCAA+CGG | - | tig0021332:41312-41331 | Msa1390170:CDS | 40.0% | |
| TGTTGGTACTTGAGTCAATG+AGG | + | tig0021332:41419-41438 | None:intergenic | 40.0% | |
| !! | AATGTTGTTGTTTGTGGTGG+TGG | - | tig0021332:41534-41553 | Msa1390170:CDS | 40.0% |
| !! | ATGTTGTTGTTTGTGGTGGT+GGG | - | tig0021332:41535-41554 | Msa1390170:CDS | 40.0% |
| !! | TAGTGGATGGAAGTGCTTTA+AGG | - | tig0021332:41565-41584 | Msa1390170:CDS | 40.0% |
| !! | AGTGGATGGAAGTGCTTTAA+GGG | - | tig0021332:41566-41585 | Msa1390170:CDS | 40.0% |
| ATCAGTTGTCTGTGAAGCAT+AGG | - | tig0021332:41592-41611 | Msa1390170:CDS | 40.0% | |
| GAGCTCAGAGAGAAAAACCT+TGG | + | tig0021332:41093-41112 | None:intergenic | 45.0% | |
| TTTCTCTCTGAGCTCTCTCA+TGG | - | tig0021332:41098-41117 | Msa1390170:CDS | 45.0% | |
| TCTCTGCTTTCTTGAGGTTG+AGG | + | tig0021332:41138-41157 | None:intergenic | 45.0% | |
| !!! | CCATGCAGCTGCTTTTACAA+GGG | + | tig0021332:41169-41188 | None:intergenic | 45.0% |
| CCTTGTAAAAGCAGCTGCAT+GGG | - | tig0021332:41167-41186 | Msa1390170:CDS | 45.0% | |
| AACTTGTAACGAGAAGGCCT+CGG | + | tig0021332:41270-41289 | None:intergenic | 45.0% | |
| TGGCCAAGGTAACTGATCTT+AGG | - | tig0021332:41304-41323 | Msa1390170:CDS | 45.0% | |
| GCACCTAAGATCAGTTACCT+TGG | + | tig0021332:41310-41329 | None:intergenic | 45.0% | |
| !! | GTGGATGGAAGTGCTTTAAG+GGG | - | tig0021332:41567-41586 | Msa1390170:CDS | 45.0% |
| CCCTTGTAAAAGCAGCTGCA+TGG | - | tig0021332:41166-41185 | Msa1390170:CDS | 50.0% | |
| !!! | CCCATGCAGCTGCTTTTACA+AGG | + | tig0021332:41170-41189 | None:intergenic | 50.0% |
| TAAAAGCAGCTGCATGGGCA+TGG | - | tig0021332:41172-41191 | Msa1390170:CDS | 50.0% | |
| AGAAGCAATGCGAATGGCCA+AGG | - | tig0021332:41290-41309 | Msa1390170:CDS | 50.0% | |
| ! | TCTTAGGTGCAACGGACTTG+AGG | - | tig0021332:41320-41339 | Msa1390170:CDS | 50.0% |
| !! | TGGTGGTGGGAATGATGTAG+TGG | - | tig0021332:41548-41567 | Msa1390170:CDS | 50.0% |
| ! | GGTGGGAATGATGTAGTGGA+TGG | - | tig0021332:41552-41571 | Msa1390170:CDS | 50.0% |
| GCATGGGCATGGTACCAACA+TGG | - | tig0021332:41183-41202 | Msa1390170:CDS | 55.0% | |
| CAAGAACTCGACGTTCACCG+AGG | - | tig0021332:41250-41269 | Msa1390170:CDS | 55.0% | |
| ! | AGGTGCAACGGACTTGAGGT+TGG | - | tig0021332:41324-41343 | Msa1390170:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021332 | gene | 41084 | 41629 | 41084 | ID=Msa1390170;Name=Msa1390170 |
| tig0021332 | mRNA | 41084 | 41629 | 41084 | ID=Msa1390170-mRNA-1;Parent=Msa1390170;Name=Msa1390170-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|181 |
| tig0021332 | exon | 41084 | 41629 | 41084 | ID=Msa1390170-mRNA-1:exon:12047;Parent=Msa1390170-mRNA-1 |
| tig0021332 | CDS | 41084 | 41629 | 41084 | ID=Msa1390170-mRNA-1:cds;Parent=Msa1390170-mRNA-1 |
| Gene Sequence |
| Protein sequence |
