AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1393000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393000 MtrunA17_Chr1g0182531 99.492 197 1 0 1 197 28 224 8.45e-145 401
Msa1393000 MtrunA17_Chr3g0077371 98.985 197 2 0 1 197 1 197 6.56e-144 398
Msa1393000 MtrunA17_Chr3g0077391 98.985 197 2 0 1 197 1 197 6.56e-144 398
Msa1393000 MtrunA17_Chr4g0009481 98.985 197 2 0 1 197 1 197 6.56e-144 398
Msa1393000 MtrunA17_Chr7g0241891 97.970 197 4 0 1 197 1 197 2.56e-142 394
Msa1393000 MtrunA17_Chr4g0060551 96.522 115 4 0 1 115 1 115 9.48e-77 225
Msa1393000 MtrunA17_Chr4g0009501 100.000 60 0 0 138 197 13 72 5.30e-38 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393000 AT5G39850.1 90.909 187 17 0 1 187 1 187 4.21e-124 348
Msa1393000 AT5G15200.1 83.838 198 31 1 1 197 1 198 9.01e-117 330
Msa1393000 AT5G15200.2 88.321 137 16 0 1 137 1 137 3.84e-85 248

Find 90 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATACATTTGATGATCTT+TGG 0.179505 tig0021591:-44204 Msa1393000:three_prime_UTR
ATCACCACCAGAAGCCTTCT+TGG 0.265320 tig0021591:+44441 None:intergenic
TACAAACACCATTAAGTAAA+AGG 0.285041 tig0021591:+44171 None:intergenic
CAATACATTTGATGATCTTT+GGG 0.308220 tig0021591:-44203 Msa1393000:three_prime_UTR
CAGACACTTGTATTCAAATC+TGG 0.328033 tig0021591:-45223 Msa1393000:CDS
AATAAACAAGGATAGGAAAA+TGG 0.330874 tig0021591:+44306 None:intergenic
CCTTCTTGGCTGCAGCCTTA+AGG 0.354248 tig0021591:+44455 None:intergenic
GTTTCATAGGGTTGGAAGGC+AGG 0.358634 tig0021591:-44587 Msa1393000:intron
GCATAGTTGAATCGAAGTTT+TGG 0.359699 tig0021591:+44087 None:intergenic
ATCATGCTAGGGTTCTTATC+AGG 0.370005 tig0021591:-45183 Msa1393000:CDS
TCGAAAATCTACCTGTGTTC+CGG 0.383091 tig0021591:-45714 Msa1393000:five_prime_UTR
CAGAATTACAAGTTCTCAAA+AGG 0.386658 tig0021591:+44044 None:intergenic
CGATTCAACTATGCTTCATA+TGG 0.393337 tig0021591:-44078 Msa1393000:three_prime_UTR
TTCGGTGCAAGAGGGAGCTT+TGG 0.394293 tig0021591:-45444 Msa1393000:CDS
GCTGCAGCCAAGAAGGCTTC+TGG 0.408125 tig0021591:-44448 Msa1393000:CDS
CCAAAGATCATCAAATGTAT+TGG 0.409949 tig0021591:+44204 None:intergenic
AACTTGTATGAACAATAAAA+AGG 0.414803 tig0021591:+44005 None:intergenic
ACAGATTCCTTGCATTGTTA+CGG 0.425233 tig0021591:+45395 None:intergenic
TCCATACGAGAAGGAGCGTT+TGG 0.432310 tig0021591:-45500 Msa1393000:CDS
GGTGAACATCCCATCCTTCA+TGG 0.437604 tig0021591:-44563 Msa1393000:CDS
CTTAAGCAAAACTCTATACT+TGG 0.438042 tig0021591:+44234 None:intergenic
CAAAAGCCCAAAACAAGAAA+AGG 0.439712 tig0021591:-45790 None:intergenic
TCCTTGCATTGTTACGGATA+CGG 0.449433 tig0021591:+45401 None:intergenic
TTCTTTCGCTTGACGCGACC+AGG 0.452567 tig0021591:+44478 None:intergenic
GGTGCAAGAGGGAGCTTTGG+AGG 0.455063 tig0021591:-45441 Msa1393000:CDS
CAAAACAAGAAAAGGCCAAT+GGG 0.457636 tig0021591:-45782 None:intergenic
AAGATAGATACTCACAGTTC+CGG 0.468521 tig0021591:+45646 None:intergenic
AGTCTAGGACAGAGAACAAA+AGG 0.472560 tig0021591:+44359 None:intergenic
TTGAAGCTGGTCGGAGAGTA+CGG 0.474460 tig0021591:-45469 Msa1393000:CDS
TAATTCTGAAACTTGTATTG+AGG 0.475054 tig0021591:-44029 Msa1393000:three_prime_UTR
TGGTTAAAGTTGCTTTCTAC+CGG 0.476762 tig0021591:-45665 Msa1393000:CDS
GAACCCTCGTCGTATCTTTG+AGG 0.479838 tig0021591:-45356 Msa1393000:CDS
GAACAAACTTGATTATGTCT+TGG 0.487846 tig0021591:-45281 Msa1393000:CDS
CCTAGCATGATGAATGGACT+TGG 0.491509 tig0021591:+45195 None:intergenic
TGGTCGGAGAGTACGGTCTT+CGG 0.495090 tig0021591:-45462 Msa1393000:CDS
AACTTTAACCATCACGCCGC+CGG 0.508542 tig0021591:+45677 None:intergenic
TGGGGTTTATAATAGAAAAC+CGG 0.516679 tig0021591:-45763 Msa1393000:five_prime_UTR
CCTTAAGGCTGCAGCCAAGA+AGG 0.517095 tig0021591:-44455 Msa1393000:CDS
AACCCTCGTCGTATCTTTGA+GGG 0.518161 tig0021591:-45355 Msa1393000:CDS
TCCAAACGCTCCTTCTCGTA+TGG 0.519540 tig0021591:+45499 None:intergenic
TTCTGGTGGTGATGGTGATG+AGG 0.523720 tig0021591:-44431 Msa1393000:CDS
AAGAACCCTAGCATGATGAA+TGG 0.528751 tig0021591:+45189 None:intergenic
GCCGGTTCTCTACTCGCCGC+CGG 0.529489 tig0021591:+45695 None:intergenic
TCAACCCTCACCATGAAGGA+TGG 0.531047 tig0021591:+44553 None:intergenic
TCCGGCGGCGAGTAGAGAAC+CGG 0.531605 tig0021591:-45696 Msa1393000:five_prime_UTR
CCAGGAAGACCACCACCAAA+AGG 0.535069 tig0021591:+44496 None:intergenic
CCAAGTCCATTCATCATGCT+AGG 0.540233 tig0021591:-45195 Msa1393000:CDS
ACTTGTATTCAAATCTGGTA+TGG 0.540887 tig0021591:-45218 Msa1393000:CDS
CGTCAAGCGAAAGAACCTTA+AGG 0.541128 tig0021591:-44470 Msa1393000:CDS
GATGCTAATTGTTTCACCAC+CGG 0.541799 tig0021591:+45744 None:intergenic
CTCGCATAATAAACAAGGAT+AGG 0.549068 tig0021591:+44299 None:intergenic
TTTGGTTTCATAGGGTTGGA+AGG 0.553079 tig0021591:-44591 Msa1393000:intron
GTGCAAGAGGGAGCTTTGGA+GGG 0.553805 tig0021591:-45440 Msa1393000:CDS
TGATAGAAAATCTAAAGTCT+AGG 0.553926 tig0021591:+44344 None:intergenic
CTATCCTTGTTTATTATGCG+AGG 0.560772 tig0021591:-44298 Msa1393000:three_prime_UTR
CCAAAACAAGAAAAGGCCAA+TGG 0.567658 tig0021591:-45783 None:intergenic
GTAGAGAACCGGCGGCGTGA+TGG 0.569580 tig0021591:-45685 Msa1393000:exon
CAACCCTCACCATGAAGGAT+GGG 0.569875 tig0021591:+44554 None:intergenic
TGAATCAACCCTCACCATGA+AGG 0.571223 tig0021591:+44549 None:intergenic
TGCAAGGAATCTGTTGACCT+TGG 0.574046 tig0021591:-45386 Msa1393000:CDS
TTATCAGGCAGAGACACATC+AGG 0.576233 tig0021591:-45168 Msa1393000:intron
AGGGTGAAGCGCTATTGAGA+AGG 0.577811 tig0021591:-45336 Msa1393000:CDS
TCTACTCGCCGCCGGAACAC+AGG 0.579171 tig0021591:+45703 None:intergenic
TTTGGATGCTGAGTTGAAGC+TGG 0.579570 tig0021591:-45482 Msa1393000:CDS
AAGAAGGCTTCTGGTGGTGA+TGG 0.583588 tig0021591:-44439 Msa1393000:CDS
AGTACGGTCTTCGGTGCAAG+AGG 0.589539 tig0021591:-45453 Msa1393000:CDS
CAAGTCCATTCATCATGCTA+GGG 0.590386 tig0021591:-45194 Msa1393000:CDS
CATCCCATCCTTCATGGTGA+GGG 0.593258 tig0021591:-44557 Msa1393000:CDS
GCCGTATCCGTAACAATGCA+AGG 0.595109 tig0021591:-45402 Msa1393000:CDS
TCATAGGGTTGGAAGGCAGG+TGG 0.599698 tig0021591:-44584 Msa1393000:intron
GCAGCCAAGAAGGCTTCTGG+TGG 0.599775 tig0021591:-44445 Msa1393000:CDS
ACATCCCATCCTTCATGGTG+AGG 0.616704 tig0021591:-44558 Msa1393000:CDS
ACGAGGGTTCTTCTCATCCA+AGG 0.618972 tig0021591:+45369 None:intergenic
TGGTGGTGATGGTGATGAGG+AGG 0.622606 tig0021591:-44428 Msa1393000:CDS
GCCGCGTCGTCCATACGAGA+AGG 0.624280 tig0021591:-45509 Msa1393000:CDS
TTTGTGTTTAAAATGTCAAG+TGG 0.624553 tig0021591:+43952 None:intergenic
TTGAGAAGGATGTTTAAGCA+TGG 0.626035 tig0021591:-45322 Msa1393000:CDS
GAGAACAAAAGGCTAGAGCA+AGG 0.632584 tig0021591:+44370 None:intergenic
ATTTCCTCGCATAATAAACA+AGG 0.635314 tig0021591:+44294 None:intergenic
AAAATCTACCTGTGTTCCGG+CGG 0.637056 tig0021591:-45711 Msa1393000:five_prime_UTR
GATGCTGAGTTGAAGCTGGT+CGG 0.640820 tig0021591:-45478 Msa1393000:CDS
ATTTGAATACAAGTGTCTGA+AGG 0.654254 tig0021591:+45227 None:intergenic
GGCGGCGAGTAGAGAACCGG+CGG 0.659354 tig0021591:-45693 Msa1393000:five_prime_UTR
TGAATACAAGTGTCTGAAGG+CGG 0.659618 tig0021591:+45230 None:intergenic
AAAACAAGAAAAGGCCAATG+GGG 0.679374 tig0021591:-45781 None:intergenic
GTACGGTCTTCGGTGCAAGA+GGG 0.685910 tig0021591:-45452 Msa1393000:CDS
GGTTTATAATAGAAAACCGG+TGG 0.690776 tig0021591:-45760 Msa1393000:five_prime_UTR
TCACCCTCAAAGATACGACG+AGG 0.726164 tig0021591:+45352 None:intergenic
CACCCTCAAAGATACGACGA+GGG 0.739733 tig0021591:+45353 None:intergenic
TCCTTCTCGTATGGACGACG+CGG 0.753773 tig0021591:+45508 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATGATAATAATGAAA+TGG + tig0021591:44099-44118 None:intergenic 10.0%
!! AAATATGAAAAATATAAACA+AGG + tig0021591:45027-45046 None:intergenic 10.0%
!! AATAAATAAATAAACATTAG+GGG + tig0021591:45065-45084 None:intergenic 10.0%
!!! ATGTATGATAATATTATTTT+TGG - tig0021591:45110-45129 Msa1393000:intron 10.0%
!! AATTAATGATAATAATGAAA+TGG + tig0021591:44099-44118 None:intergenic 10.0%
!! AAATATGAAAAATATAAACA+AGG + tig0021591:45027-45046 None:intergenic 10.0%
!! AATAAATAAATAAACATTAG+GGG + tig0021591:45065-45084 None:intergenic 10.0%
!!! ATGTATGATAATATTATTTT+TGG - tig0021591:45110-45129 Msa1393000:intron 10.0%
!!! AATTATCATTTTACATGAAT+TGG - tig0021591:44591-44610 Msa1393000:intron 15.0%
!!! TTTGAGTATTTTAATTAAAC+AGG + tig0021591:44886-44905 None:intergenic 15.0%
!!! AATATTATTTTTGGTTTCAT+AGG - tig0021591:45119-45138 Msa1393000:intron 15.0%
!!! ATATTATTTTTGGTTTCATA+GGG - tig0021591:45120-45139 Msa1393000:intron 15.0%
!!! AATTATCATTTTACATGAAT+TGG - tig0021591:44591-44610 Msa1393000:intron 15.0%
!!! TTTGAGTATTTTAATTAAAC+AGG + tig0021591:44886-44905 None:intergenic 15.0%
!!! AATATTATTTTTGGTTTCAT+AGG - tig0021591:45119-45138 Msa1393000:intron 15.0%
!!! ATATTATTTTTGGTTTCATA+GGG - tig0021591:45120-45139 Msa1393000:intron 15.0%
!!! GTACAATTGTTTTGAATTTA+TGG - tig0021591:44135-44154 Msa1393000:three_prime_UTR 20.0%
!! CAAAACATGTATTGAATTAA+AGG + tig0021591:44677-44696 None:intergenic 20.0%
!!! ATTTTTTTTGTTACTCTGAA+TGG - tig0021591:44999-45018 Msa1393000:intron 20.0%
!!! TTCATATTTTGTATGCTTAT+TGG - tig0021591:45038-45057 Msa1393000:intron 20.0%
!!! TAATTGAACCTTTTACTTAA+TGG - tig0021591:45540-45559 Msa1393000:intron 20.0%
!!! TTTGATTTTGCATATTTTGA+TGG - tig0021591:45586-45605 Msa1393000:intron 20.0%
!!! TTGATTTTGCATATTTTGAT+GGG - tig0021591:45587-45606 Msa1393000:intron 20.0%
!! AACTTGTATGAACAATAAAA+AGG + tig0021591:45717-45736 None:intergenic 20.0%
!!! GTACAATTGTTTTGAATTTA+TGG - tig0021591:44135-44154 Msa1393000:three_prime_UTR 20.0%
!! CAAAACATGTATTGAATTAA+AGG + tig0021591:44677-44696 None:intergenic 20.0%
!!! ATTTTTTTTGTTACTCTGAA+TGG - tig0021591:44999-45018 Msa1393000:intron 20.0%
!!! TTCATATTTTGTATGCTTAT+TGG - tig0021591:45038-45057 Msa1393000:intron 20.0%
!!! TAATTGAACCTTTTACTTAA+TGG - tig0021591:45540-45559 Msa1393000:intron 20.0%
!!! TTTGATTTTGCATATTTTGA+TGG - tig0021591:45586-45605 Msa1393000:intron 20.0%
!!! TTGATTTTGCATATTTTGAT+GGG - tig0021591:45587-45606 Msa1393000:intron 20.0%
!! AACTTGTATGAACAATAAAA+AGG + tig0021591:45717-45736 None:intergenic 20.0%
!! TTTTCTATTATAAACCCCAT+TGG + tig0021591:43955-43974 None:intergenic 25.0%
! TTACATGAATTGGAAAAAAC+AGG - tig0021591:44601-44620 Msa1393000:intron 25.0%
! CAATATATTCTATGTATCGA+AGG - tig0021591:44824-44843 Msa1393000:intron 25.0%
!! TAGTCTTAACGAAATATGTT+TGG - tig0021591:45085-45104 Msa1393000:intron 25.0%
! AGTCTTAACGAAATATGTTT+GGG - tig0021591:45086-45105 Msa1393000:intron 25.0%
!! TGATAGAAAATCTAAAGTCT+AGG + tig0021591:45378-45397 None:intergenic 25.0%
! AATAAACAAGGATAGGAAAA+TGG + tig0021591:45416-45435 None:intergenic 25.0%
!! CAATACATTTGATGATCTTT+GGG - tig0021591:45516-45535 Msa1393000:CDS 25.0%
! TACAAACACCATTAAGTAAA+AGG + tig0021591:45551-45570 None:intergenic 25.0%
!!! TGATTTTGCATATTTTGATG+GGG - tig0021591:45588-45607 Msa1393000:intron 25.0%
!!! TAATTCTGAAACTTGTATTG+AGG - tig0021591:45690-45709 Msa1393000:five_prime_UTR 25.0%
!! TTTGTGTTTAAAATGTCAAG+TGG + tig0021591:45770-45789 None:intergenic 25.0%
!! TTTTCTATTATAAACCCCAT+TGG + tig0021591:43955-43974 None:intergenic 25.0%
! TTACATGAATTGGAAAAAAC+AGG - tig0021591:44601-44620 Msa1393000:intron 25.0%
! CAATATATTCTATGTATCGA+AGG - tig0021591:44824-44843 Msa1393000:intron 25.0%
!! TAGTCTTAACGAAATATGTT+TGG - tig0021591:45085-45104 Msa1393000:intron 25.0%
! AGTCTTAACGAAATATGTTT+GGG - tig0021591:45086-45105 Msa1393000:intron 25.0%
!! TGATAGAAAATCTAAAGTCT+AGG + tig0021591:45378-45397 None:intergenic 25.0%
! AATAAACAAGGATAGGAAAA+TGG + tig0021591:45416-45435 None:intergenic 25.0%
!! CAATACATTTGATGATCTTT+GGG - tig0021591:45516-45535 Msa1393000:CDS 25.0%
! TACAAACACCATTAAGTAAA+AGG + tig0021591:45551-45570 None:intergenic 25.0%
!!! TGATTTTGCATATTTTGATG+GGG - tig0021591:45588-45607 Msa1393000:intron 25.0%
!!! TAATTCTGAAACTTGTATTG+AGG - tig0021591:45690-45709 Msa1393000:five_prime_UTR 25.0%
!! TTTGTGTTTAAAATGTCAAG+TGG + tig0021591:45770-45789 None:intergenic 25.0%
TGGGGTTTATAATAGAAAAC+CGG - tig0021591:43956-43975 Msa1393000:three_prime_UTR 30.0%
ATTGAATACACATACACAGA+TGG - tig0021591:44172-44191 Msa1393000:three_prime_UTR 30.0%
TTGAATACACATACACAGAT+GGG - tig0021591:44173-44192 Msa1393000:three_prime_UTR 30.0%
GAACAAACTTGATTATGTCT+TGG - tig0021591:44438-44457 Msa1393000:CDS 30.0%
ATTTGAATACAAGTGTCTGA+AGG + tig0021591:44495-44514 None:intergenic 30.0%
ACTTGTATTCAAATCTGGTA+TGG - tig0021591:44501-44520 Msa1393000:CDS 30.0%
! ACAGGAAAGTATATGTGAAA+TGG - tig0021591:44619-44638 Msa1393000:intron 30.0%
ATATGTGAAATGGAGTATAC+AGG - tig0021591:44629-44648 Msa1393000:intron 30.0%
!!! GTAACTTGCATTTTTTCTAG+CGG - tig0021591:44782-44801 Msa1393000:intron 30.0%
!!! TACTCAAATGTCACTGATTT+TGG - tig0021591:44898-44917 Msa1393000:intron 30.0%
!!! TATTTTTGGTTTCATAGGGT+TGG - tig0021591:45124-45143 Msa1393000:intron 30.0%
ATTTCCTCGCATAATAAACA+AGG + tig0021591:45428-45447 None:intergenic 30.0%
CTTAAGCAAAACTCTATACT+TGG + tig0021591:45488-45507 None:intergenic 30.0%
CCAAAGATCATCAAATGTAT+TGG + tig0021591:45518-45537 None:intergenic 30.0%
! CCAATACATTTGATGATCTT+TGG - tig0021591:45515-45534 Msa1393000:CDS 30.0%
CAGAATTACAAGTTCTCAAA+AGG + tig0021591:45678-45697 None:intergenic 30.0%
TGGGGTTTATAATAGAAAAC+CGG - tig0021591:43956-43975 Msa1393000:three_prime_UTR 30.0%
ATTGAATACACATACACAGA+TGG - tig0021591:44172-44191 Msa1393000:three_prime_UTR 30.0%
TTGAATACACATACACAGAT+GGG - tig0021591:44173-44192 Msa1393000:three_prime_UTR 30.0%
GAACAAACTTGATTATGTCT+TGG - tig0021591:44438-44457 Msa1393000:CDS 30.0%
ATTTGAATACAAGTGTCTGA+AGG + tig0021591:44495-44514 None:intergenic 30.0%
ACTTGTATTCAAATCTGGTA+TGG - tig0021591:44501-44520 Msa1393000:CDS 30.0%
! ACAGGAAAGTATATGTGAAA+TGG - tig0021591:44619-44638 Msa1393000:intron 30.0%
ATATGTGAAATGGAGTATAC+AGG - tig0021591:44629-44648 Msa1393000:intron 30.0%
!!! GTAACTTGCATTTTTTCTAG+CGG - tig0021591:44782-44801 Msa1393000:intron 30.0%
!!! TACTCAAATGTCACTGATTT+TGG - tig0021591:44898-44917 Msa1393000:intron 30.0%
!!! TATTTTTGGTTTCATAGGGT+TGG - tig0021591:45124-45143 Msa1393000:intron 30.0%
ATTTCCTCGCATAATAAACA+AGG + tig0021591:45428-45447 None:intergenic 30.0%
CTTAAGCAAAACTCTATACT+TGG + tig0021591:45488-45507 None:intergenic 30.0%
CCAAAGATCATCAAATGTAT+TGG + tig0021591:45518-45537 None:intergenic 30.0%
! CCAATACATTTGATGATCTT+TGG - tig0021591:45515-45534 Msa1393000:CDS 30.0%
CAGAATTACAAGTTCTCAAA+AGG + tig0021591:45678-45697 None:intergenic 30.0%
GGTTTATAATAGAAAACCGG+TGG - tig0021591:43959-43978 Msa1393000:three_prime_UTR 35.0%
! TGGTTAAAGTTGCTTTCTAC+CGG - tig0021591:44054-44073 Msa1393000:three_prime_UTR 35.0%
AAGATAGATACTCACAGTTC+CGG + tig0021591:44076-44095 None:intergenic 35.0%
ACAGATTCCTTGCATTGTTA+CGG + tig0021591:44327-44346 None:intergenic 35.0%
TTGAGAAGGATGTTTAAGCA+TGG - tig0021591:44397-44416 Msa1393000:three_prime_UTR 35.0%
CAGACACTTGTATTCAAATC+TGG - tig0021591:44496-44515 Msa1393000:CDS 35.0%
!! TTCAAGTTGCCAGCAAAAAT+GGG + tig0021591:44713-44732 None:intergenic 35.0%
!!! ATTTTTTCTAGCGGCTGTTA+TGG - tig0021591:44791-44810 Msa1393000:intron 35.0%
TTCTATGTATCGAAGGTAGT+AGG - tig0021591:44831-44850 Msa1393000:intron 35.0%
! TCCATTGTTCAAGTAATGGA+GGG - tig0021591:44860-44879 Msa1393000:intron 35.0%
! TTTTCACTCACAAGTCCTTT+TGG - tig0021591:45208-45227 Msa1393000:CDS 35.0%
CTCGCATAATAAACAAGGAT+AGG + tig0021591:45423-45442 None:intergenic 35.0%
! CTATCCTTGTTTATTATGCG+AGG - tig0021591:45421-45440 Msa1393000:CDS 35.0%
!! AGTTGAATCGAAGTTTTGGT+TGG + tig0021591:45631-45650 None:intergenic 35.0%
! GCATAGTTGAATCGAAGTTT+TGG + tig0021591:45635-45654 None:intergenic 35.0%
CGATTCAACTATGCTTCATA+TGG - tig0021591:45641-45660 Msa1393000:intron 35.0%
GGTTTATAATAGAAAACCGG+TGG - tig0021591:43959-43978 Msa1393000:three_prime_UTR 35.0%
! TGGTTAAAGTTGCTTTCTAC+CGG - tig0021591:44054-44073 Msa1393000:three_prime_UTR 35.0%
AAGATAGATACTCACAGTTC+CGG + tig0021591:44076-44095 None:intergenic 35.0%
ACAGATTCCTTGCATTGTTA+CGG + tig0021591:44327-44346 None:intergenic 35.0%
TTGAGAAGGATGTTTAAGCA+TGG - tig0021591:44397-44416 Msa1393000:three_prime_UTR 35.0%
CAGACACTTGTATTCAAATC+TGG - tig0021591:44496-44515 Msa1393000:CDS 35.0%
!! TTCAAGTTGCCAGCAAAAAT+GGG + tig0021591:44713-44732 None:intergenic 35.0%
!!! ATTTTTTCTAGCGGCTGTTA+TGG - tig0021591:44791-44810 Msa1393000:intron 35.0%
TTCTATGTATCGAAGGTAGT+AGG - tig0021591:44831-44850 Msa1393000:intron 35.0%
! TCCATTGTTCAAGTAATGGA+GGG - tig0021591:44860-44879 Msa1393000:intron 35.0%
! TTTTCACTCACAAGTCCTTT+TGG - tig0021591:45208-45227 Msa1393000:CDS 35.0%
CTCGCATAATAAACAAGGAT+AGG + tig0021591:45423-45442 None:intergenic 35.0%
! CTATCCTTGTTTATTATGCG+AGG - tig0021591:45421-45440 Msa1393000:CDS 35.0%
!! AGTTGAATCGAAGTTTTGGT+TGG + tig0021591:45631-45650 None:intergenic 35.0%
! GCATAGTTGAATCGAAGTTT+TGG + tig0021591:45635-45654 None:intergenic 35.0%
CGATTCAACTATGCTTCATA+TGG - tig0021591:45641-45660 Msa1393000:intron 35.0%
GATGCTAATTGTTTCACCAC+CGG + tig0021591:43978-43997 None:intergenic 40.0%
! CACAGGTAGATTTTCGAAGA+AGG + tig0021591:44002-44021 None:intergenic 40.0%
TCGAAAATCTACCTGTGTTC+CGG - tig0021591:44005-44024 Msa1393000:three_prime_UTR 40.0%
TCCTTGCATTGTTACGGATA+CGG + tig0021591:44321-44340 None:intergenic 40.0%
TGAATACAAGTGTCTGAAGG+CGG + tig0021591:44492-44511 None:intergenic 40.0%
CAAGTCCATTCATCATGCTA+GGG - tig0021591:44525-44544 Msa1393000:CDS 40.0%
AAGAACCCTAGCATGATGAA+TGG + tig0021591:44533-44552 None:intergenic 40.0%
ATCATGCTAGGGTTCTTATC+AGG - tig0021591:44536-44555 Msa1393000:CDS 40.0%
! TCAAGTTGCCAGCAAAAATG+GGG + tig0021591:44712-44731 None:intergenic 40.0%
!! GTTCAAGTTGCCAGCAAAAA+TGG + tig0021591:44714-44733 None:intergenic 40.0%
!! GACTAAAGCTACTGCTTTTC+TGG - tig0021591:44738-44757 Msa1393000:intron 40.0%
CCATTACTTGAACAATGGAG+AGG + tig0021591:44859-44878 None:intergenic 40.0%
CCTCTCCATTGTTCAAGTAA+TGG - tig0021591:44856-44875 Msa1393000:intron 40.0%
CTCCATTGTTCAAGTAATGG+AGG - tig0021591:44859-44878 Msa1393000:intron 40.0%
GTGAATGAAGTGAAGAGAGA+TGG - tig0021591:44965-44984 Msa1393000:intron 40.0%
! TTTGGTTTCATAGGGTTGGA+AGG - tig0021591:45128-45147 Msa1393000:intron 40.0%
! AGTCTAGGACAGAGAACAAA+AGG + tig0021591:45363-45382 None:intergenic 40.0%
!! TTTTGGTTGGTTGGATACAC+AGG + tig0021591:45618-45637 None:intergenic 40.0%
!!! GAATCGAAGTTTTGGTTGGT+TGG + tig0021591:45627-45646 None:intergenic 40.0%
GATGCTAATTGTTTCACCAC+CGG + tig0021591:43978-43997 None:intergenic 40.0%
! CACAGGTAGATTTTCGAAGA+AGG + tig0021591:44002-44021 None:intergenic 40.0%
TCGAAAATCTACCTGTGTTC+CGG - tig0021591:44005-44024 Msa1393000:three_prime_UTR 40.0%
TCCTTGCATTGTTACGGATA+CGG + tig0021591:44321-44340 None:intergenic 40.0%
TGAATACAAGTGTCTGAAGG+CGG + tig0021591:44492-44511 None:intergenic 40.0%
CAAGTCCATTCATCATGCTA+GGG - tig0021591:44525-44544 Msa1393000:CDS 40.0%
AAGAACCCTAGCATGATGAA+TGG + tig0021591:44533-44552 None:intergenic 40.0%
ATCATGCTAGGGTTCTTATC+AGG - tig0021591:44536-44555 Msa1393000:CDS 40.0%
! TCAAGTTGCCAGCAAAAATG+GGG + tig0021591:44712-44731 None:intergenic 40.0%
!! GTTCAAGTTGCCAGCAAAAA+TGG + tig0021591:44714-44733 None:intergenic 40.0%
!! GACTAAAGCTACTGCTTTTC+TGG - tig0021591:44738-44757 Msa1393000:intron 40.0%
CCATTACTTGAACAATGGAG+AGG + tig0021591:44859-44878 None:intergenic 40.0%
CCTCTCCATTGTTCAAGTAA+TGG - tig0021591:44856-44875 Msa1393000:intron 40.0%
CTCCATTGTTCAAGTAATGG+AGG - tig0021591:44859-44878 Msa1393000:intron 40.0%
GTGAATGAAGTGAAGAGAGA+TGG - tig0021591:44965-44984 Msa1393000:intron 40.0%
! TTTGGTTTCATAGGGTTGGA+AGG - tig0021591:45128-45147 Msa1393000:intron 40.0%
! AGTCTAGGACAGAGAACAAA+AGG + tig0021591:45363-45382 None:intergenic 40.0%
!! TTTTGGTTGGTTGGATACAC+AGG + tig0021591:45618-45637 None:intergenic 40.0%
!!! GAATCGAAGTTTTGGTTGGT+TGG + tig0021591:45627-45646 None:intergenic 40.0%
AAAATCTACCTGTGTTCCGG+CGG - tig0021591:44008-44027 Msa1393000:three_prime_UTR 45.0%
! TTTGGATGCTGAGTTGAAGC+TGG - tig0021591:44237-44256 Msa1393000:three_prime_UTR 45.0%
TGCAAGGAATCTGTTGACCT+TGG - tig0021591:44333-44352 Msa1393000:three_prime_UTR 45.0%
AACCCTCGTCGTATCTTTGA+GGG - tig0021591:44364-44383 Msa1393000:three_prime_UTR 45.0%
! CCTAGCATGATGAATGGACT+TGG + tig0021591:44527-44546 None:intergenic 45.0%
CCAAGTCCATTCATCATGCT+AGG - tig0021591:44524-44543 Msa1393000:CDS 45.0%
TTATCAGGCAGAGACACATC+AGG - tig0021591:44551-44570 Msa1393000:CDS 45.0%
!! TGCTATTGCCCCATTTTTGC+TGG - tig0021591:44701-44720 Msa1393000:intron 45.0%
!!! CTGCTTTTCTGGCTTTTCTC+TGG - tig0021591:44749-44768 Msa1393000:intron 45.0%
GCCCTCCATTACTTGAACAA+TGG + tig0021591:44864-44883 None:intergenic 45.0%
TGAATCAACCCTCACCATGA+AGG + tig0021591:45173-45192 None:intergenic 45.0%
! TCACTCACAAGTCCTTTTGG+TGG - tig0021591:45211-45230 Msa1393000:CDS 45.0%
CGTCAAGCGAAAGAACCTTA+AGG - tig0021591:45249-45268 Msa1393000:CDS 45.0%
GAGAACAAAAGGCTAGAGCA+AGG + tig0021591:45352-45371 None:intergenic 45.0%
AAAATCTACCTGTGTTCCGG+CGG - tig0021591:44008-44027 Msa1393000:three_prime_UTR 45.0%
! TTTGGATGCTGAGTTGAAGC+TGG - tig0021591:44237-44256 Msa1393000:three_prime_UTR 45.0%
TGCAAGGAATCTGTTGACCT+TGG - tig0021591:44333-44352 Msa1393000:three_prime_UTR 45.0%
AACCCTCGTCGTATCTTTGA+GGG - tig0021591:44364-44383 Msa1393000:three_prime_UTR 45.0%
! CCTAGCATGATGAATGGACT+TGG + tig0021591:44527-44546 None:intergenic 45.0%
CCAAGTCCATTCATCATGCT+AGG - tig0021591:44524-44543 Msa1393000:CDS 45.0%
TTATCAGGCAGAGACACATC+AGG - tig0021591:44551-44570 Msa1393000:CDS 45.0%
!! TGCTATTGCCCCATTTTTGC+TGG - tig0021591:44701-44720 Msa1393000:intron 45.0%
!!! CTGCTTTTCTGGCTTTTCTC+TGG - tig0021591:44749-44768 Msa1393000:intron 45.0%
GCCCTCCATTACTTGAACAA+TGG + tig0021591:44864-44883 None:intergenic 45.0%
TGAATCAACCCTCACCATGA+AGG + tig0021591:45173-45192 None:intergenic 45.0%
! TCACTCACAAGTCCTTTTGG+TGG - tig0021591:45211-45230 Msa1393000:CDS 45.0%
CGTCAAGCGAAAGAACCTTA+AGG - tig0021591:45249-45268 Msa1393000:CDS 45.0%
GAGAACAAAAGGCTAGAGCA+AGG + tig0021591:45352-45371 None:intergenic 45.0%
!! AAATAAATAAATAAACATTA+GGG + tig0021591:45066-45085 None:intergenic 5.0%
!! TAAATAAATAAATAAACATT+AGG + tig0021591:45067-45086 None:intergenic 5.0%
!! AAATAAATAAATAAACATTA+GGG + tig0021591:45066-45085 None:intergenic 5.0%
!! TAAATAAATAAATAAACATT+AGG + tig0021591:45067-45086 None:intergenic 5.0%
AACTTTAACCATCACGCCGC+CGG + tig0021591:44045-44064 None:intergenic 50.0%
!! TCCATACGAGAAGGAGCGTT+TGG - tig0021591:44219-44238 Msa1393000:three_prime_UTR 50.0%
TCCAAACGCTCCTTCTCGTA+TGG + tig0021591:44223-44242 None:intergenic 50.0%
! GATGCTGAGTTGAAGCTGGT+CGG - tig0021591:44241-44260 Msa1393000:three_prime_UTR 50.0%
TTGAAGCTGGTCGGAGAGTA+CGG - tig0021591:44250-44269 Msa1393000:three_prime_UTR 50.0%
GCCGTATCCGTAACAATGCA+AGG - tig0021591:44317-44336 Msa1393000:three_prime_UTR 50.0%
ACGAGGGTTCTTCTCATCCA+AGG + tig0021591:44353-44372 None:intergenic 50.0%
GAACCCTCGTCGTATCTTTG+AGG - tig0021591:44363-44382 Msa1393000:three_prime_UTR 50.0%
CACCCTCAAAGATACGACGA+GGG + tig0021591:44369-44388 None:intergenic 50.0%
TCACCCTCAAAGATACGACG+AGG + tig0021591:44370-44389 None:intergenic 50.0%
! AGGGTGAAGCGCTATTGAGA+AGG - tig0021591:44383-44402 Msa1393000:three_prime_UTR 50.0%
GTTTCATAGGGTTGGAAGGC+AGG - tig0021591:45132-45151 Msa1393000:intron 50.0%
GGTGAACATCCCATCCTTCA+TGG - tig0021591:45156-45175 Msa1393000:intron 50.0%
ACATCCCATCCTTCATGGTG+AGG - tig0021591:45161-45180 Msa1393000:intron 50.0%
CATCCCATCCTTCATGGTGA+GGG - tig0021591:45162-45181 Msa1393000:intron 50.0%
CAACCCTCACCATGAAGGAT+GGG + tig0021591:45168-45187 None:intergenic 50.0%
TCAACCCTCACCATGAAGGA+TGG + tig0021591:45169-45188 None:intergenic 50.0%
! CTCACAAGTCCTTTTGGTGG+TGG - tig0021591:45214-45233 Msa1393000:CDS 50.0%
ATCACCACCAGAAGCCTTCT+TGG + tig0021591:45281-45300 None:intergenic 50.0%
! AAGAAGGCTTCTGGTGGTGA+TGG - tig0021591:45280-45299 Msa1393000:CDS 50.0%
!! TTCTGGTGGTGATGGTGATG+AGG - tig0021591:45288-45307 Msa1393000:CDS 50.0%
AACTTTAACCATCACGCCGC+CGG + tig0021591:44045-44064 None:intergenic 50.0%
!! TCCATACGAGAAGGAGCGTT+TGG - tig0021591:44219-44238 Msa1393000:three_prime_UTR 50.0%
TCCAAACGCTCCTTCTCGTA+TGG + tig0021591:44223-44242 None:intergenic 50.0%
! GATGCTGAGTTGAAGCTGGT+CGG - tig0021591:44241-44260 Msa1393000:three_prime_UTR 50.0%
TTGAAGCTGGTCGGAGAGTA+CGG - tig0021591:44250-44269 Msa1393000:three_prime_UTR 50.0%
GCCGTATCCGTAACAATGCA+AGG - tig0021591:44317-44336 Msa1393000:three_prime_UTR 50.0%
ACGAGGGTTCTTCTCATCCA+AGG + tig0021591:44353-44372 None:intergenic 50.0%
GAACCCTCGTCGTATCTTTG+AGG - tig0021591:44363-44382 Msa1393000:three_prime_UTR 50.0%
CACCCTCAAAGATACGACGA+GGG + tig0021591:44369-44388 None:intergenic 50.0%
TCACCCTCAAAGATACGACG+AGG + tig0021591:44370-44389 None:intergenic 50.0%
! AGGGTGAAGCGCTATTGAGA+AGG - tig0021591:44383-44402 Msa1393000:three_prime_UTR 50.0%
GTTTCATAGGGTTGGAAGGC+AGG - tig0021591:45132-45151 Msa1393000:intron 50.0%
GGTGAACATCCCATCCTTCA+TGG - tig0021591:45156-45175 Msa1393000:intron 50.0%
ACATCCCATCCTTCATGGTG+AGG - tig0021591:45161-45180 Msa1393000:intron 50.0%
CATCCCATCCTTCATGGTGA+GGG - tig0021591:45162-45181 Msa1393000:intron 50.0%
CAACCCTCACCATGAAGGAT+GGG + tig0021591:45168-45187 None:intergenic 50.0%
TCAACCCTCACCATGAAGGA+TGG + tig0021591:45169-45188 None:intergenic 50.0%
! CTCACAAGTCCTTTTGGTGG+TGG - tig0021591:45214-45233 Msa1393000:CDS 50.0%
ATCACCACCAGAAGCCTTCT+TGG + tig0021591:45281-45300 None:intergenic 50.0%
! AAGAAGGCTTCTGGTGGTGA+TGG - tig0021591:45280-45299 Msa1393000:CDS 50.0%
!! TTCTGGTGGTGATGGTGATG+AGG - tig0021591:45288-45307 Msa1393000:CDS 50.0%
TCCTTCTCGTATGGACGACG+CGG + tig0021591:44214-44233 None:intergenic 55.0%
! TGGTCGGAGAGTACGGTCTT+CGG - tig0021591:44257-44276 Msa1393000:three_prime_UTR 55.0%
! AGTACGGTCTTCGGTGCAAG+AGG - tig0021591:44266-44285 Msa1393000:three_prime_UTR 55.0%
! GTACGGTCTTCGGTGCAAGA+GGG - tig0021591:44267-44286 Msa1393000:three_prime_UTR 55.0%
! TTCGGTGCAAGAGGGAGCTT+TGG - tig0021591:44275-44294 Msa1393000:three_prime_UTR 55.0%
!! GTGCAAGAGGGAGCTTTGGA+GGG - tig0021591:44279-44298 Msa1393000:three_prime_UTR 55.0%
TCATAGGGTTGGAAGGCAGG+TGG - tig0021591:45135-45154 Msa1393000:intron 55.0%
CCAGGAAGACCACCACCAAA+AGG + tig0021591:45226-45245 None:intergenic 55.0%
!!! CCTTTTGGTGGTGGTCTTCC+TGG - tig0021591:45223-45242 Msa1393000:CDS 55.0%
TTCTTTCGCTTGACGCGACC+AGG + tig0021591:45244-45263 None:intergenic 55.0%
CCTTCTTGGCTGCAGCCTTA+AGG + tig0021591:45267-45286 None:intergenic 55.0%
CCTTAAGGCTGCAGCCAAGA+AGG - tig0021591:45264-45283 Msa1393000:CDS 55.0%
!! TGGTGGTGATGGTGATGAGG+AGG - tig0021591:45291-45310 Msa1393000:CDS 55.0%
TCCTTCTCGTATGGACGACG+CGG + tig0021591:44214-44233 None:intergenic 55.0%
! TGGTCGGAGAGTACGGTCTT+CGG - tig0021591:44257-44276 Msa1393000:three_prime_UTR 55.0%
! AGTACGGTCTTCGGTGCAAG+AGG - tig0021591:44266-44285 Msa1393000:three_prime_UTR 55.0%
! GTACGGTCTTCGGTGCAAGA+GGG - tig0021591:44267-44286 Msa1393000:three_prime_UTR 55.0%
! TTCGGTGCAAGAGGGAGCTT+TGG - tig0021591:44275-44294 Msa1393000:three_prime_UTR 55.0%
!! GTGCAAGAGGGAGCTTTGGA+GGG - tig0021591:44279-44298 Msa1393000:three_prime_UTR 55.0%
TCATAGGGTTGGAAGGCAGG+TGG - tig0021591:45135-45154 Msa1393000:intron 55.0%
CCAGGAAGACCACCACCAAA+AGG + tig0021591:45226-45245 None:intergenic 55.0%
!!! CCTTTTGGTGGTGGTCTTCC+TGG - tig0021591:45223-45242 Msa1393000:CDS 55.0%
TTCTTTCGCTTGACGCGACC+AGG + tig0021591:45244-45263 None:intergenic 55.0%
CCTTCTTGGCTGCAGCCTTA+AGG + tig0021591:45267-45286 None:intergenic 55.0%
CCTTAAGGCTGCAGCCAAGA+AGG - tig0021591:45264-45283 Msa1393000:CDS 55.0%
!! TGGTGGTGATGGTGATGAGG+AGG - tig0021591:45291-45310 Msa1393000:CDS 55.0%
! GGTGCAAGAGGGAGCTTTGG+AGG - tig0021591:44278-44297 Msa1393000:three_prime_UTR 60.0%
GCTGCAGCCAAGAAGGCTTC+TGG - tig0021591:45271-45290 Msa1393000:CDS 60.0%
GCAGCCAAGAAGGCTTCTGG+TGG - tig0021591:45274-45293 Msa1393000:CDS 60.0%
! GGTGCAAGAGGGAGCTTTGG+AGG - tig0021591:44278-44297 Msa1393000:three_prime_UTR 60.0%
GCTGCAGCCAAGAAGGCTTC+TGG - tig0021591:45271-45290 Msa1393000:CDS 60.0%
GCAGCCAAGAAGGCTTCTGG+TGG - tig0021591:45274-45293 Msa1393000:CDS 60.0%
TCTACTCGCCGCCGGAACAC+AGG + tig0021591:44019-44038 None:intergenic 65.0%
TCCGGCGGCGAGTAGAGAAC+CGG - tig0021591:44023-44042 Msa1393000:three_prime_UTR 65.0%
GTAGAGAACCGGCGGCGTGA+TGG - tig0021591:44034-44053 Msa1393000:three_prime_UTR 65.0%
GCCGCGTCGTCCATACGAGA+AGG - tig0021591:44210-44229 Msa1393000:three_prime_UTR 65.0%
TCTACTCGCCGCCGGAACAC+AGG + tig0021591:44019-44038 None:intergenic 65.0%
TCCGGCGGCGAGTAGAGAAC+CGG - tig0021591:44023-44042 Msa1393000:three_prime_UTR 65.0%
GTAGAGAACCGGCGGCGTGA+TGG - tig0021591:44034-44053 Msa1393000:three_prime_UTR 65.0%
GCCGCGTCGTCCATACGAGA+AGG - tig0021591:44210-44229 Msa1393000:three_prime_UTR 65.0%
! GCCGGTTCTCTACTCGCCGC+CGG + tig0021591:44027-44046 None:intergenic 70.0%
GGCGGCGAGTAGAGAACCGG+CGG - tig0021591:44026-44045 Msa1393000:three_prime_UTR 70.0%
! GCCGGTTCTCTACTCGCCGC+CGG + tig0021591:44027-44046 None:intergenic 70.0%
GGCGGCGAGTAGAGAACCGG+CGG - tig0021591:44026-44045 Msa1393000:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
tig0021591 gene 43942 45799 43942 ID=Msa1393000;Name=Msa1393000
tig0021591 mRNA 43942 45799 43942 ID=Msa1393000-mRNA-1;Parent=Msa1393000;Name=Msa1393000-mRNA-1;_AED=0.01;_eAED=0.01;_QI=111|1|1|1|1|1|3|475|197
tig0021591 exon 43942 44600 43942 ID=Msa1393000-mRNA-1:exon:13582;Parent=Msa1393000-mRNA-1
tig0021591 exon 45169 45550 45169 ID=Msa1393000-mRNA-1:exon:13581;Parent=Msa1393000-mRNA-1
tig0021591 exon 45661 45799 45661 ID=Msa1393000-mRNA-1:exon:13580;Parent=Msa1393000-mRNA-1
tig0021591 five_prime_UTR 45689 45799 45689 ID=Msa1393000-mRNA-1:five_prime_utr;Parent=Msa1393000-mRNA-1
tig0021591 CDS 45661 45688 45661 ID=Msa1393000-mRNA-1:cds;Parent=Msa1393000-mRNA-1
tig0021591 CDS 45169 45550 45169 ID=Msa1393000-mRNA-1:cds;Parent=Msa1393000-mRNA-1
tig0021591 CDS 44417 44600 44417 ID=Msa1393000-mRNA-1:cds;Parent=Msa1393000-mRNA-1
tig0021591 three_prime_UTR 43942 44416 43942 ID=Msa1393000-mRNA-1:three_prime_utr;Parent=Msa1393000-mRNA-1
Gene Sequence

>Msa1393000

ATGGTTAAAGTTGCTTTCTACCGGAACTATGGGAAAACATTCAAGAAGCCGCGTCGTCCATACGAGAAGGAGCGTTTGGATGCTGAGTTGAAGCTGGTCGGAGAGTACGGTCTTCGGTGCAAGAGGGAGCTTTGGAGGGTTCAGTATGCTCTGAGCCGTATCCGTAACAATGCAAGGAATCTGTTGACCTTGGATGAGAAGAACCCTCGTCGTATCTTTGAGGGTGAAGCGCTATTGAGAAGGATGTTTAAGCATGGTCTTCTTGATGAAACTCAGAACAAACTTGATTATGTCTTGGCTCTCACTGTTGAGAATTTTCTTGAACGCCGCCTTCAGACACTTGTATTCAAATCTGGTATGGCCAAGTCCATTCATCATGCTAGGGTTCTTATCAGGCAGAGACACATCAGGGTTGGAAGGCAGGTGGTGAACATCCCATCCTTCATGGTGAGGGTTGATTCACAGAAGCACATTGACTTTTCACTCACAAGTCCTTTTGGTGGTGGTCTTCCTGGTCGCGTCAAGCGAAAGAACCTTAAGGCTGCAGCCAAGAAGGCTTCTGGTGGTGATGGTGATGAGGAGGATGAAGATTAA
Protein sequence

>Msa1393000

MVKVAFYRNYGKTFKKPRRPYEKERLDAELKLVGEYGLRCKRELWRVQYALSRIRNNARNLLTLDEKNPRRIFEGEALLRRMFKHGLLDETQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQVVNIPSFMVRVDSQKHIDFSLTSPFGGGLPGRVKRKNLKAAAKKASGGDGDEEDED*