AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0140850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCTGGAATTTCTGAATT+TGG 0.202611 1_3:+75390301 Msa0140850:three_prime_UTR
TCTCTCTCGTATTGATGTTT+TGG 0.256448 1_3:+75390185 Msa0140850:three_prime_UTR
TTGATATTGTTATATTGTTT+TGG 0.263717 1_3:+75390350 Msa0140850:three_prime_UTR
TGGATCTCTGTCTCATCTTT+CGG 0.332419 1_3:-75390051 None:intergenic
TGCTTCTGCGCAGTCTCATT+AGG 0.363924 1_3:+75389853 Msa0140850:five_prime_UTR
ATTATGTGTATGTTTATTAG+TGG 0.373896 1_3:+75390496 Msa0140850:three_prime_UTR
AGTCCTAAAACCACAACAAT+TGG 0.396442 1_3:-75390387 None:intergenic
GTGTATGTTTATTAGTGGTT+AGG 0.414479 1_3:+75390501 Msa0140850:three_prime_UTR
CGCATCTTGTACCAATGAAA+TGG 0.418125 1_3:+75390431 Msa0140850:three_prime_UTR
GCGCAGAAGCAACTGCGATT+AGG 0.428523 1_3:-75389841 None:intergenic
CTTCGAGTGTTAGTCATGTT+AGG 0.437316 1_3:+75390408 Msa0140850:three_prime_UTR
AGCGTATGAGGAGGTTGAAG+AGG 0.488055 1_3:+75390023 Msa0140850:CDS
AAGAACACCAACATACTACT+TGG 0.523061 1_3:-75390071 None:intergenic
AGTGGAAGAAGAAGCGTATG+AGG 0.538884 1_3:+75390011 Msa0140850:intron
TTCAACAATCATGCGTGCCA+AGG 0.540770 1_3:+75389903 Msa0140850:exon
GCACGCATGATTGTTGAAAA+CGG 0.548829 1_3:-75389898 None:intergenic
ATATGTTAAGAGAGTAGTAT+CGG 0.555824 1_3:+75390156 Msa0140850:three_prime_UTR
TTGTTGAAATCCAATTGTTG+TGG 0.563688 1_3:+75390377 Msa0140850:three_prime_UTR
CAGAGATCCAAGTAGTATGT+TGG 0.564238 1_3:+75390064 Msa0140850:exon
AATATCAAAGATGAAAGCAT+AGG 0.592277 1_3:-75390335 None:intergenic
CCCATCACTTGTCTAAAATG+TGG 0.601993 1_3:+75390574 Msa0140850:three_prime_UTR
GACAAGTGATGGGATCACCA+TGG 0.660164 1_3:-75390564 None:intergenic
GGAAGAAGAAGCGTATGAGG+AGG 0.665498 1_3:+75390014 Msa0140850:CDS
ATAAGTCTGATTAAACAACA+CGG 0.774353 1_3:-75390132 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTTATAGTTATAATAA+TGG + chr1_3:75389951-75389970 Msa0140850:intron 10.0%
!!! TTTTGTTATAGTTATAATAA+TGG + chr1_3:75389951-75389970 Msa0140850:intron 10.0%
!! AAAACAAAAATTAATTGCAA+TGG - chr1_3:75389935-75389954 None:intergenic 15.0%
!!! TATAGTTATAATAATGGTTT+TGG + chr1_3:75389957-75389976 Msa0140850:intron 15.0%
!!! TTTGATTTTGATTTTGATTT+TGG + chr1_3:75389980-75389999 Msa0140850:intron 15.0%
!!! TGTTTATTTTTTTGATTTTG+CGG + chr1_3:75390280-75390299 Msa0140850:three_prime_UTR 15.0%
!!! TTGATATTGTTATATTGTTT+TGG + chr1_3:75390350-75390369 Msa0140850:three_prime_UTR 15.0%
!! AAAACAAAAATTAATTGCAA+TGG - chr1_3:75389935-75389954 None:intergenic 15.0%
!!! TATAGTTATAATAATGGTTT+TGG + chr1_3:75389957-75389976 Msa0140850:intron 15.0%
!!! TTTGATTTTGATTTTGATTT+TGG + chr1_3:75389980-75389999 Msa0140850:intron 15.0%
!!! TGTTTATTTTTTTGATTTTG+CGG + chr1_3:75390280-75390299 Msa0140850:three_prime_UTR 15.0%
!!! TTGATATTGTTATATTGTTT+TGG + chr1_3:75390350-75390369 Msa0140850:three_prime_UTR 15.0%
!!! CTCAAAGATATTTTTCTTTA+TGG + chr1_3:75390219-75390238 Msa0140850:three_prime_UTR 20.0%
!! TAAAAAATAAGCCATTTCAT+TGG - chr1_3:75390445-75390464 None:intergenic 20.0%
!! ATTATGTGTATGTTTATTAG+TGG + chr1_3:75390496-75390515 Msa0140850:three_prime_UTR 20.0%
!!! CTCAAAGATATTTTTCTTTA+TGG + chr1_3:75390219-75390238 Msa0140850:three_prime_UTR 20.0%
!! TAAAAAATAAGCCATTTCAT+TGG - chr1_3:75390445-75390464 None:intergenic 20.0%
!! ATTATGTGTATGTTTATTAG+TGG + chr1_3:75390496-75390515 Msa0140850:three_prime_UTR 20.0%
! ATAAGTCTGATTAAACAACA+CGG - chr1_3:75390135-75390154 None:intergenic 25.0%
! ATATGTTAAGAGAGTAGTAT+CGG + chr1_3:75390156-75390175 Msa0140850:three_prime_UTR 25.0%
!! AATATCAAAGATGAAAGCAT+AGG - chr1_3:75390338-75390357 None:intergenic 25.0%
!!! TATTGTTATATTGTTTTGGC+TGG + chr1_3:75390354-75390373 Msa0140850:three_prime_UTR 25.0%
! GTTTCTTAAAAACAAAAGTG+TGG - chr1_3:75390545-75390564 None:intergenic 25.0%
!!! CTTTTGTTTTTAAGAAACCA+TGG + chr1_3:75390547-75390566 Msa0140850:three_prime_UTR 25.0%
! ATAAGTCTGATTAAACAACA+CGG - chr1_3:75390135-75390154 None:intergenic 25.0%
! ATATGTTAAGAGAGTAGTAT+CGG + chr1_3:75390156-75390175 Msa0140850:three_prime_UTR 25.0%
!! AATATCAAAGATGAAAGCAT+AGG - chr1_3:75390338-75390357 None:intergenic 25.0%
!!! TATTGTTATATTGTTTTGGC+TGG + chr1_3:75390354-75390373 Msa0140850:three_prime_UTR 25.0%
! GTTTCTTAAAAACAAAAGTG+TGG - chr1_3:75390545-75390564 None:intergenic 25.0%
!!! CTTTTGTTTTTAAGAAACCA+TGG + chr1_3:75390547-75390566 Msa0140850:three_prime_UTR 25.0%
!!! TTTTTTTGATTTTGCGGTTC+TGG + chr1_3:75390286-75390305 Msa0140850:three_prime_UTR 30.0%
TTGTTGAAATCCAATTGTTG+TGG + chr1_3:75390377-75390396 Msa0140850:three_prime_UTR 30.0%
! AATCCAATTGTTGTGGTTTT+AGG + chr1_3:75390384-75390403 Msa0140850:three_prime_UTR 30.0%
GTGTATGTTTATTAGTGGTT+AGG + chr1_3:75390501-75390520 Msa0140850:three_prime_UTR 30.0%
!!! TTTTTTTGATTTTGCGGTTC+TGG + chr1_3:75390286-75390305 Msa0140850:three_prime_UTR 30.0%
TTGTTGAAATCCAATTGTTG+TGG + chr1_3:75390377-75390396 Msa0140850:three_prime_UTR 30.0%
! AATCCAATTGTTGTGGTTTT+AGG + chr1_3:75390384-75390403 Msa0140850:three_prime_UTR 30.0%
GTGTATGTTTATTAGTGGTT+AGG + chr1_3:75390501-75390520 Msa0140850:three_prime_UTR 30.0%
AATTGCAATGGATCTAACCT+TGG - chr1_3:75389923-75389942 None:intergenic 35.0%
! TTGATTTTGGATTTGTGCAG+TGG + chr1_3:75389993-75390012 Msa0140850:intron 35.0%
AAGAACACCAACATACTACT+TGG - chr1_3:75390074-75390093 None:intergenic 35.0%
!! TCTCTCTCGTATTGATGTTT+TGG + chr1_3:75390185-75390204 Msa0140850:three_prime_UTR 35.0%
!! GGTTCTGGAATTTCTGAATT+TGG + chr1_3:75390301-75390320 Msa0140850:three_prime_UTR 35.0%
AGTCCTAAAACCACAACAAT+TGG - chr1_3:75390390-75390409 None:intergenic 35.0%
! CCACATTTTAGACAAGTGAT+GGG - chr1_3:75390577-75390596 None:intergenic 35.0%
AATTGCAATGGATCTAACCT+TGG - chr1_3:75389923-75389942 None:intergenic 35.0%
! TTGATTTTGGATTTGTGCAG+TGG + chr1_3:75389993-75390012 Msa0140850:intron 35.0%
AAGAACACCAACATACTACT+TGG - chr1_3:75390074-75390093 None:intergenic 35.0%
!! TCTCTCTCGTATTGATGTTT+TGG + chr1_3:75390185-75390204 Msa0140850:three_prime_UTR 35.0%
!! GGTTCTGGAATTTCTGAATT+TGG + chr1_3:75390301-75390320 Msa0140850:three_prime_UTR 35.0%
AGTCCTAAAACCACAACAAT+TGG - chr1_3:75390390-75390409 None:intergenic 35.0%
! CCACATTTTAGACAAGTGAT+GGG - chr1_3:75390577-75390596 None:intergenic 35.0%
GCACGCATGATTGTTGAAAA+CGG - chr1_3:75389901-75389920 None:intergenic 40.0%
TGGATCTCTGTCTCATCTTT+CGG - chr1_3:75390054-75390073 None:intergenic 40.0%
CAGAGATCCAAGTAGTATGT+TGG + chr1_3:75390064-75390083 Msa0140850:exon 40.0%
CTTCGAGTGTTAGTCATGTT+AGG + chr1_3:75390408-75390427 Msa0140850:three_prime_UTR 40.0%
CGCATCTTGTACCAATGAAA+TGG + chr1_3:75390431-75390450 Msa0140850:three_prime_UTR 40.0%
! CCCATCACTTGTCTAAAATG+TGG + chr1_3:75390574-75390593 Msa0140850:three_prime_UTR 40.0%
! GCCACATTTTAGACAAGTGA+TGG - chr1_3:75390578-75390597 None:intergenic 40.0%
GCACGCATGATTGTTGAAAA+CGG - chr1_3:75389901-75389920 None:intergenic 40.0%
TGGATCTCTGTCTCATCTTT+CGG - chr1_3:75390054-75390073 None:intergenic 40.0%
CAGAGATCCAAGTAGTATGT+TGG + chr1_3:75390064-75390083 Msa0140850:exon 40.0%
CTTCGAGTGTTAGTCATGTT+AGG + chr1_3:75390408-75390427 Msa0140850:three_prime_UTR 40.0%
CGCATCTTGTACCAATGAAA+TGG + chr1_3:75390431-75390450 Msa0140850:three_prime_UTR 40.0%
! CCCATCACTTGTCTAAAATG+TGG + chr1_3:75390574-75390593 Msa0140850:three_prime_UTR 40.0%
! GCCACATTTTAGACAAGTGA+TGG - chr1_3:75390578-75390597 None:intergenic 40.0%
TTCAACAATCATGCGTGCCA+AGG + chr1_3:75389903-75389922 Msa0140850:exon 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG + chr1_3:75390011-75390030 Msa0140850:intron 45.0%
TTCAACAATCATGCGTGCCA+AGG + chr1_3:75389903-75389922 Msa0140850:exon 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG + chr1_3:75390011-75390030 Msa0140850:intron 45.0%
TGCTTCTGCGCAGTCTCATT+AGG + chr1_3:75389853-75389872 Msa0140850:five_prime_UTR 50.0%
! GGAAGAAGAAGCGTATGAGG+AGG + chr1_3:75390014-75390033 Msa0140850:CDS 50.0%
AGCGTATGAGGAGGTTGAAG+AGG + chr1_3:75390023-75390042 Msa0140850:CDS 50.0%
! GACAAGTGATGGGATCACCA+TGG - chr1_3:75390567-75390586 None:intergenic 50.0%
TGCTTCTGCGCAGTCTCATT+AGG + chr1_3:75389853-75389872 Msa0140850:five_prime_UTR 50.0%
! GGAAGAAGAAGCGTATGAGG+AGG + chr1_3:75390014-75390033 Msa0140850:CDS 50.0%
AGCGTATGAGGAGGTTGAAG+AGG + chr1_3:75390023-75390042 Msa0140850:CDS 50.0%
! GACAAGTGATGGGATCACCA+TGG - chr1_3:75390567-75390586 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 75389844 75390660 75389844 ID=Msa0140850;Name=Msa0140850
chr1_3 mRNA 75389844 75390660 75389844 ID=Msa0140850-mRNA-1;Parent=Msa0140850;Name=Msa0140850-mRNA-1;_AED=0.00;_eAED=0.00;_QI=69|1|1|1|1|1|2|582|25
chr1_3 exon 75389844 75389924 75389844 ID=Msa0140850-mRNA-1:exon:26302;Parent=Msa0140850-mRNA-1
chr1_3 exon 75390013 75390660 75390013 ID=Msa0140850-mRNA-1:exon:26303;Parent=Msa0140850-mRNA-1
chr1_3 five_prime_UTR 75389844 75389912 75389844 ID=Msa0140850-mRNA-1:five_prime_utr;Parent=Msa0140850-mRNA-1
chr1_3 CDS 75389913 75389924 75389913 ID=Msa0140850-mRNA-1:cds;Parent=Msa0140850-mRNA-1
chr1_3 CDS 75390013 75390078 75390013 ID=Msa0140850-mRNA-1:cds;Parent=Msa0140850-mRNA-1
chr1_3 three_prime_UTR 75390079 75390660 75390079 ID=Msa0140850-mRNA-1:three_prime_utr;Parent=Msa0140850-mRNA-1
Gene Sequence

>Msa0140850

ATGCGTGCCAAGTGGAAGAAGAAGCGTATGAGGAGGTTGAAGAGGAAGCGCCGAAAGATGAGACAGAGATCCAAGTAG

Protein sequence

>Msa0140850

MRAKWKKKRMRRLKRKRRKMRQRSK*