AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0172930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172930 MtrunA17_Chr1g0195471 91.597 119 9 1 7 125 2 119 2.23e-65 194
Msa0172930 MtrunA17_Chr7g0272321 94.444 108 6 0 18 125 17 124 4.39e-62 186
Msa0172930 MtrunA17_Chr1g0192241 98.305 59 1 0 67 125 18 76 2.61e-26 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172930 AT3G05560.1 84.800 125 18 1 1 125 1 124 3.87e-63 188
Msa0172930 AT3G05560.3 84.800 125 18 1 1 125 1 124 3.87e-63 188
Msa0172930 AT3G05560.2 84.800 125 18 1 1 125 1 124 3.87e-63 188
Msa0172930 AT5G27770.1 84.694 98 15 0 17 114 16 113 3.97e-49 153
Msa0172930 AT1G02830.1 69.492 118 33 2 1 116 1 117 8.11e-43 137

Find 44 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCGCCTTCTATACTCTTTC+TGG 0.191248 1_4:-58118840 Msa0172930:three_prime_UTR
CGGCAGTGGCAGAAGGGATT+AGG 0.267881 1_4:+58120506 None:intergenic
GTGATGCTGTTACTGTTTCC+AGG 0.305504 1_4:-58120155 Msa0172930:CDS
TCTTATGTTAGCTTCTCTTT+TGG 0.316391 1_4:-58118988 Msa0172930:three_prime_UTR
GATCTTGTCTTCCACGGGTT+TGG 0.336765 1_4:+58120248 None:intergenic
GAGAAGCTAACATAAGAAAA+TGG 0.346112 1_4:+58118994 None:intergenic
GTTTCTTCAAGAGAGGATTA+AGG 0.367162 1_4:-58120202 Msa0172930:CDS
TTCTGTTCTCTTGTGGCTAA+AGG 0.373476 1_4:-58120444 Msa0172930:intron
GTTACCAGAAAGAGTATAGA+AGG 0.386657 1_4:+58118836 None:intergenic
AAAACGCGCGTTTCACTTCG+TGG 0.396594 1_4:+58120548 None:intergenic
ACTTGAAGAAACACAATGTA+AGG 0.413403 1_4:-58119151 Msa0172930:CDS
GGCAGTGGCAGAAGGGATTA+GGG 0.414585 1_4:+58120507 None:intergenic
CACGGGTTTGGCACAGTCAA+TGG 0.421170 1_4:+58120260 None:intergenic
GGTGGTAAACCTGGTGCTCT+TGG 0.432206 1_4:-58120177 Msa0172930:CDS
GGCAGAAGGGATTAGGGTAT+AGG 0.432402 1_4:+58120513 None:intergenic
GTCCATGATCTTGTCTTCCA+CGG 0.443029 1_4:+58120242 None:intergenic
ATGCTCGAGACATGTTGCTT+TGG 0.463943 1_4:-58118958 Msa0172930:three_prime_UTR
AAAATAATCTTAGACATTCA+AGG 0.465501 1_4:+58118808 None:intergenic
AAGGGATTAGGGTATAGGTT+TGG 0.481938 1_4:+58120518 None:intergenic
TGTTCTGTTCTGTTCTCTTG+TGG 0.495042 1_4:-58120451 Msa0172930:five_prime_UTR
ATTAAGGTTGGTGGTAAACC+TGG 0.503896 1_4:-58120186 Msa0172930:CDS
GTAGATGCAGCTGGTTCAAA+GGG 0.506848 1_4:-58120309 Msa0172930:CDS
TGGTTCAAAGGGAAAGAAGA+AGG 0.510234 1_4:-58120298 Msa0172930:CDS
CTGATAGCAACTTCTCCAAA+CGG 0.520527 1_4:-58120107 Msa0172930:intron
AGAAACACAATGTAAGGGAT+TGG 0.522187 1_4:-58119145 Msa0172930:CDS
TCCATGATCTTGTCTTCCAC+GGG 0.522466 1_4:+58120243 None:intergenic
TCGAAAAGTTTCTTCAAGAG+AGG 0.523656 1_4:-58120209 Msa0172930:CDS
CAAGAGAGGATTAAGGTTGG+TGG 0.536618 1_4:-58120195 Msa0172930:CDS
AGTAGATGCAGCTGGTTCAA+AGG 0.536758 1_4:-58120310 Msa0172930:CDS
AAATGGACGGCAGTGGCAGA+AGG 0.545088 1_4:+58120499 None:intergenic
TTCAACATTGCTGAGAATGA+AGG 0.551218 1_4:-58119071 Msa0172930:CDS
ACCCGTGGAAGACAAGATCA+TGG 0.560910 1_4:-58120244 Msa0172930:CDS
CTTCAAGAGAGGATTAAGGT+TGG 0.564429 1_4:-58120198 Msa0172930:CDS
ATGGGTCGAGTAGATGCAGC+TGG 0.584016 1_4:-58120318 Msa0172930:CDS
CAGAACACAGCACAATGTGA+AGG 0.593257 1_4:+58120472 None:intergenic
ACAGCATCACCAAGAGCACC+AGG 0.602480 1_4:+58120168 None:intergenic
ATAGAAATGTTTATGAGCTG+AGG 0.605170 1_4:-58119097 Msa0172930:CDS
AATGGACGGCAGTGGCAGAA+GGG 0.610217 1_4:+58120500 None:intergenic
TGATGCTGTTACTGTTTCCA+GGG 0.614918 1_4:-58120154 Msa0172930:CDS
AGAACACAGCACAATGTGAA+GGG 0.618440 1_4:+58120473 None:intergenic
GGTTCAAAGGGAAAGAAGAA+GGG 0.618619 1_4:-58120297 Msa0172930:CDS
CATTGACTGTGCCAAACCCG+TGG 0.627134 1_4:-58120259 Msa0172930:CDS
CTTGAAGAAACACAATGTAA+GGG 0.629064 1_4:-58119150 Msa0172930:CDS
CATTGCTGAGAATGAAGGAG+AGG 0.630620 1_4:-58119066 Msa0172930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGATTAATTGAAATGAAA+GGG + chr1_4:58118959-58118978 None:intergenic 15.0%
!! TTATGATTAATTGAAATGAA+AGG + chr1_4:58118960-58118979 None:intergenic 15.0%
!!! GGATAATTTTTTTGAAAATA+AGG + chr1_4:58119986-58120005 None:intergenic 15.0%
!! TATGATTAATTGAAATGAAA+GGG + chr1_4:58118959-58118978 None:intergenic 15.0%
!! TTATGATTAATTGAAATGAA+AGG + chr1_4:58118960-58118979 None:intergenic 15.0%
!!! GGATAATTTTTTTGAAAATA+AGG + chr1_4:58119986-58120005 None:intergenic 15.0%
!! ATGATTAATTGAAATGAAAG+GGG + chr1_4:58118958-58118977 None:intergenic 20.0%
!!! AGTTTTTTGTTTTGTTTGAT+TGG - chr1_4:58119321-58119340 Msa0172930:intron 20.0%
!!! ATTTAGGTTGTTTTTTATGA+TGG - chr1_4:58119382-58119401 Msa0172930:intron 20.0%
!!! AGTGTTAAAATTGATTCTAA+AGG + chr1_4:58119587-58119606 None:intergenic 20.0%
!!! TGATTTTGTAAAACTAATGT+TGG + chr1_4:58119653-58119672 None:intergenic 20.0%
!!! TTATGTTAACTTTTGATTCA+TGG - chr1_4:58119831-58119850 Msa0172930:intron 20.0%
!!! CCAATAATATTAGAAGTTTT+TGG + chr1_4:58119897-58119916 None:intergenic 20.0%
!! CCAAAAACTTCTAATATTAT+TGG - chr1_4:58119894-58119913 Msa0172930:intron 20.0%
!!! TTTTGTTCAATTAAGAATTG+CGG - chr1_4:58120447-58120466 Msa0172930:five_prime_UTR 20.0%
!! AAAATAATCTTAGACATTCA+AGG + chr1_4:58120554-58120573 None:intergenic 20.0%
!! ATGATTAATTGAAATGAAAG+GGG + chr1_4:58118958-58118977 None:intergenic 20.0%
!!! AGTTTTTTGTTTTGTTTGAT+TGG - chr1_4:58119321-58119340 Msa0172930:intron 20.0%
!!! ATTTAGGTTGTTTTTTATGA+TGG - chr1_4:58119382-58119401 Msa0172930:intron 20.0%
!!! AGTGTTAAAATTGATTCTAA+AGG + chr1_4:58119587-58119606 None:intergenic 20.0%
!!! TGATTTTGTAAAACTAATGT+TGG + chr1_4:58119653-58119672 None:intergenic 20.0%
!!! TTATGTTAACTTTTGATTCA+TGG - chr1_4:58119831-58119850 Msa0172930:intron 20.0%
!!! CCAATAATATTAGAAGTTTT+TGG + chr1_4:58119897-58119916 None:intergenic 20.0%
!! CCAAAAACTTCTAATATTAT+TGG - chr1_4:58119894-58119913 Msa0172930:intron 20.0%
!!! TTTTGTTCAATTAAGAATTG+CGG - chr1_4:58120447-58120466 Msa0172930:five_prime_UTR 20.0%
!! AAAATAATCTTAGACATTCA+AGG + chr1_4:58120554-58120573 None:intergenic 20.0%
! TCATTCATAGAAGACAAAAA+TGG - chr1_4:58119022-58119041 Msa0172930:three_prime_UTR 25.0%
! CATTCATAGAAGACAAAAAT+GGG - chr1_4:58119023-58119042 Msa0172930:three_prime_UTR 25.0%
!!! TTTTTGTTTTGTTTGATTGG+AGG - chr1_4:58119324-58119343 Msa0172930:intron 25.0%
!! GGAATTCAATTGTTTGATTT+AGG - chr1_4:58119366-58119385 Msa0172930:intron 25.0%
!! TAAAATTGATTCTAAAGGTG+TGG + chr1_4:58119582-58119601 None:intergenic 25.0%
!!! AATTTTAACACTACTAGAAG+TGG - chr1_4:58119595-58119614 Msa0172930:intron 25.0%
!!! ATTGATTTTACAATCTCGTT+TGG - chr1_4:58119716-58119735 Msa0172930:intron 25.0%
!! TTGATTTTACAATCTCGTTT+GGG - chr1_4:58119717-58119736 Msa0172930:intron 25.0%
!! ATATTATTGGCAATTTTGTG+TGG - chr1_4:58119907-58119926 Msa0172930:intron 25.0%
!! TATTATTGGCAATTTTGTGT+GGG - chr1_4:58119908-58119927 Msa0172930:intron 25.0%
! TCATTCATAGAAGACAAAAA+TGG - chr1_4:58119022-58119041 Msa0172930:three_prime_UTR 25.0%
! CATTCATAGAAGACAAAAAT+GGG - chr1_4:58119023-58119042 Msa0172930:three_prime_UTR 25.0%
!!! TTTTTGTTTTGTTTGATTGG+AGG - chr1_4:58119324-58119343 Msa0172930:intron 25.0%
!! GGAATTCAATTGTTTGATTT+AGG - chr1_4:58119366-58119385 Msa0172930:intron 25.0%
!! TAAAATTGATTCTAAAGGTG+TGG + chr1_4:58119582-58119601 None:intergenic 25.0%
!!! AATTTTAACACTACTAGAAG+TGG - chr1_4:58119595-58119614 Msa0172930:intron 25.0%
!!! ATTGATTTTACAATCTCGTT+TGG - chr1_4:58119716-58119735 Msa0172930:intron 25.0%
!! TTGATTTTACAATCTCGTTT+GGG - chr1_4:58119717-58119736 Msa0172930:intron 25.0%
!! ATATTATTGGCAATTTTGTG+TGG - chr1_4:58119907-58119926 Msa0172930:intron 25.0%
!! TATTATTGGCAATTTTGTGT+GGG - chr1_4:58119908-58119927 Msa0172930:intron 25.0%
AATTGAAATGAAAGGGGAAA+AGG + chr1_4:58118952-58118971 None:intergenic 30.0%
TCGAGATTCAATTTCAGTTT+CGG + chr1_4:58118995-58119014 None:intergenic 30.0%
AAGAATAGAGAATAACCGTT+TGG + chr1_4:58119270-58119289 None:intergenic 30.0%
! GGATTTGTGTTTTCTTAGTT+AGG - chr1_4:58119345-58119364 Msa0172930:intron 30.0%
!!! TGTTTTTTATGATGGTTCTG+CGG - chr1_4:58119390-58119409 Msa0172930:intron 30.0%
!!! AATGTGTGTGTTTGGTTTTA+GGG + chr1_4:58119699-58119718 None:intergenic 30.0%
GTAAAATCAATGTGTGTGTT+TGG + chr1_4:58119707-58119726 None:intergenic 30.0%
TTCATGGAATATACAATCTC+TGG - chr1_4:58119847-58119866 Msa0172930:intron 30.0%
TCATGGAATATACAATCTCT+GGG - chr1_4:58119848-58119867 Msa0172930:intron 30.0%
! ATTATTGGCAATTTTGTGTG+GGG - chr1_4:58119909-58119928 Msa0172930:intron 30.0%
TTCTGTCTAAATTGCAATGA+TGG - chr1_4:58119937-58119956 Msa0172930:intron 30.0%
TCTGTCTAAATTGCAATGAT+GGG - chr1_4:58119938-58119957 Msa0172930:intron 30.0%
! ACACAGTTTTTGTGATCTAA+AGG - chr1_4:58120121-58120140 Msa0172930:CDS 30.0%
ACGAGTTGTTAAAAAAAGCA+TGG + chr1_4:58120152-58120171 None:intergenic 30.0%
! GTGTTTCTTCAAGTACTTTT+TGG + chr1_4:58120202-58120221 None:intergenic 30.0%
ACTTGAAGAAACACAATGTA+AGG - chr1_4:58120208-58120227 Msa0172930:CDS 30.0%
CTTGAAGAAACACAATGTAA+GGG - chr1_4:58120209-58120228 Msa0172930:CDS 30.0%
ATAGAAATGTTTATGAGCTG+AGG - chr1_4:58120262-58120281 Msa0172930:CDS 30.0%
GAGAAGCTAACATAAGAAAA+TGG + chr1_4:58120368-58120387 None:intergenic 30.0%
!! TCTTATGTTAGCTTCTCTTT+TGG - chr1_4:58120371-58120390 Msa0172930:intron 30.0%
AATTGAAATGAAAGGGGAAA+AGG + chr1_4:58118952-58118971 None:intergenic 30.0%
TCGAGATTCAATTTCAGTTT+CGG + chr1_4:58118995-58119014 None:intergenic 30.0%
AAGAATAGAGAATAACCGTT+TGG + chr1_4:58119270-58119289 None:intergenic 30.0%
! GGATTTGTGTTTTCTTAGTT+AGG - chr1_4:58119345-58119364 Msa0172930:intron 30.0%
!!! TGTTTTTTATGATGGTTCTG+CGG - chr1_4:58119390-58119409 Msa0172930:intron 30.0%
!!! AATGTGTGTGTTTGGTTTTA+GGG + chr1_4:58119699-58119718 None:intergenic 30.0%
GTAAAATCAATGTGTGTGTT+TGG + chr1_4:58119707-58119726 None:intergenic 30.0%
TTCATGGAATATACAATCTC+TGG - chr1_4:58119847-58119866 Msa0172930:intron 30.0%
TCATGGAATATACAATCTCT+GGG - chr1_4:58119848-58119867 Msa0172930:intron 30.0%
! ATTATTGGCAATTTTGTGTG+GGG - chr1_4:58119909-58119928 Msa0172930:intron 30.0%
TTCTGTCTAAATTGCAATGA+TGG - chr1_4:58119937-58119956 Msa0172930:intron 30.0%
TCTGTCTAAATTGCAATGAT+GGG - chr1_4:58119938-58119957 Msa0172930:intron 30.0%
! ACACAGTTTTTGTGATCTAA+AGG - chr1_4:58120121-58120140 Msa0172930:CDS 30.0%
ACGAGTTGTTAAAAAAAGCA+TGG + chr1_4:58120152-58120171 None:intergenic 30.0%
! GTGTTTCTTCAAGTACTTTT+TGG + chr1_4:58120202-58120221 None:intergenic 30.0%
ACTTGAAGAAACACAATGTA+AGG - chr1_4:58120208-58120227 Msa0172930:CDS 30.0%
CTTGAAGAAACACAATGTAA+GGG - chr1_4:58120209-58120228 Msa0172930:CDS 30.0%
ATAGAAATGTTTATGAGCTG+AGG - chr1_4:58120262-58120281 Msa0172930:CDS 30.0%
GAGAAGCTAACATAAGAAAA+TGG + chr1_4:58120368-58120387 None:intergenic 30.0%
!! TCTTATGTTAGCTTCTCTTT+TGG - chr1_4:58120371-58120390 Msa0172930:intron 30.0%
ATGTGAAGGGAAAAAATGGA+CGG + chr1_4:58118876-58118895 None:intergenic 35.0%
CACAATGTGAAGGGAAAAAA+TGG + chr1_4:58118880-58118899 None:intergenic 35.0%
AGATTCAATTTCAGTTTCGG+TGG + chr1_4:58118992-58119011 None:intergenic 35.0%
TCGAAAAGTTTCTTCAAGAG+AGG - chr1_4:58119150-58119169 Msa0172930:CDS 35.0%
GTTTCTTCAAGAGAGGATTA+AGG - chr1_4:58119157-58119176 Msa0172930:CDS 35.0%
!!! AGTAATCTTGCTTTTTTCCC+TGG + chr1_4:58119225-58119244 None:intergenic 35.0%
TCCGATTAAAAGTGAGTTGT+AGG - chr1_4:58119441-58119460 Msa0172930:intron 35.0%
!! CCCTACAACTCACTTTTAAT+CGG + chr1_4:58119445-58119464 None:intergenic 35.0%
CCGATTAAAAGTGAGTTGTA+GGG - chr1_4:58119442-58119461 Msa0172930:intron 35.0%
TGAATTGAAGATGGTAGGTT+TGG + chr1_4:58119623-58119642 None:intergenic 35.0%
AAGAGTGAATTGAAGATGGT+AGG + chr1_4:58119628-58119647 None:intergenic 35.0%
!!! CAATGTGTGTGTTTGGTTTT+AGG + chr1_4:58119700-58119719 None:intergenic 35.0%
! ATTTTACAATCTCGTTTGGG+TGG - chr1_4:58119720-58119739 Msa0172930:intron 35.0%
CATATTGCGATGATGCTAAA+AGG + chr1_4:58119772-58119791 None:intergenic 35.0%
AAATTATCCCCTGCTTCAAA+AGG - chr1_4:58119997-58120016 Msa0172930:intron 35.0%
!!! AACAAAAGCCTTTTGAAGCA+GGG + chr1_4:58120008-58120027 None:intergenic 35.0%
TGCAATTGTTCGTATTCAGA+TGG - chr1_4:58120031-58120050 Msa0172930:intron 35.0%
GCAATTGTTCGTATTCAGAT+GGG - chr1_4:58120032-58120051 Msa0172930:intron 35.0%
CAATTGTTCGTATTCAGATG+GGG - chr1_4:58120033-58120052 Msa0172930:intron 35.0%
TTAACAACTCGTATTGTTCC+AGG - chr1_4:58120160-58120179 Msa0172930:CDS 35.0%
AGAAACACAATGTAAGGGAT+TGG - chr1_4:58120214-58120233 Msa0172930:CDS 35.0%
TTCAACATTGCTGAGAATGA+AGG - chr1_4:58120288-58120307 Msa0172930:CDS 35.0%
! AATTGCGGTAACACTTTTGT+TGG - chr1_4:58120462-58120481 Msa0172930:five_prime_UTR 35.0%
! GTTACCAGAAAGAGTATAGA+AGG + chr1_4:58120526-58120545 None:intergenic 35.0%
ATGTGAAGGGAAAAAATGGA+CGG + chr1_4:58118876-58118895 None:intergenic 35.0%
CACAATGTGAAGGGAAAAAA+TGG + chr1_4:58118880-58118899 None:intergenic 35.0%
AGATTCAATTTCAGTTTCGG+TGG + chr1_4:58118992-58119011 None:intergenic 35.0%
TCGAAAAGTTTCTTCAAGAG+AGG - chr1_4:58119150-58119169 Msa0172930:CDS 35.0%
GTTTCTTCAAGAGAGGATTA+AGG - chr1_4:58119157-58119176 Msa0172930:CDS 35.0%
!!! AGTAATCTTGCTTTTTTCCC+TGG + chr1_4:58119225-58119244 None:intergenic 35.0%
TCCGATTAAAAGTGAGTTGT+AGG - chr1_4:58119441-58119460 Msa0172930:intron 35.0%
!! CCCTACAACTCACTTTTAAT+CGG + chr1_4:58119445-58119464 None:intergenic 35.0%
CCGATTAAAAGTGAGTTGTA+GGG - chr1_4:58119442-58119461 Msa0172930:intron 35.0%
TGAATTGAAGATGGTAGGTT+TGG + chr1_4:58119623-58119642 None:intergenic 35.0%
AAGAGTGAATTGAAGATGGT+AGG + chr1_4:58119628-58119647 None:intergenic 35.0%
!!! CAATGTGTGTGTTTGGTTTT+AGG + chr1_4:58119700-58119719 None:intergenic 35.0%
! ATTTTACAATCTCGTTTGGG+TGG - chr1_4:58119720-58119739 Msa0172930:intron 35.0%
CATATTGCGATGATGCTAAA+AGG + chr1_4:58119772-58119791 None:intergenic 35.0%
AAATTATCCCCTGCTTCAAA+AGG - chr1_4:58119997-58120016 Msa0172930:intron 35.0%
!!! AACAAAAGCCTTTTGAAGCA+GGG + chr1_4:58120008-58120027 None:intergenic 35.0%
TGCAATTGTTCGTATTCAGA+TGG - chr1_4:58120031-58120050 Msa0172930:intron 35.0%
GCAATTGTTCGTATTCAGAT+GGG - chr1_4:58120032-58120051 Msa0172930:intron 35.0%
CAATTGTTCGTATTCAGATG+GGG - chr1_4:58120033-58120052 Msa0172930:intron 35.0%
TTAACAACTCGTATTGTTCC+AGG - chr1_4:58120160-58120179 Msa0172930:CDS 35.0%
AGAAACACAATGTAAGGGAT+TGG - chr1_4:58120214-58120233 Msa0172930:CDS 35.0%
TTCAACATTGCTGAGAATGA+AGG - chr1_4:58120288-58120307 Msa0172930:CDS 35.0%
! AATTGCGGTAACACTTTTGT+TGG - chr1_4:58120462-58120481 Msa0172930:five_prime_UTR 35.0%
! GTTACCAGAAAGAGTATAGA+AGG + chr1_4:58120526-58120545 None:intergenic 35.0%
AAGGGATTAGGGTATAGGTT+TGG + chr1_4:58118844-58118863 None:intergenic 40.0%
AGAACACAGCACAATGTGAA+GGG + chr1_4:58118889-58118908 None:intergenic 40.0%
TGTTCTGTTCTGTTCTCTTG+TGG - chr1_4:58118908-58118927 Msa0172930:three_prime_UTR 40.0%
TTCTGTTCTCTTGTGGCTAA+AGG - chr1_4:58118915-58118934 Msa0172930:three_prime_UTR 40.0%
TGGTTCAAAGGGAAAGAAGA+AGG - chr1_4:58119061-58119080 Msa0172930:CDS 40.0%
GGTTCAAAGGGAAAGAAGAA+GGG - chr1_4:58119062-58119081 Msa0172930:CDS 40.0%
CTTCAAGAGAGGATTAAGGT+TGG - chr1_4:58119161-58119180 Msa0172930:CDS 40.0%
!! ATTAAGGTTGGTGGTAAACC+TGG - chr1_4:58119173-58119192 Msa0172930:CDS 40.0%
TGATGCTGTTACTGTTTCCA+GGG - chr1_4:58119205-58119224 Msa0172930:intron 40.0%
CTGATAGCAACTTCTCCAAA+CGG - chr1_4:58119252-58119271 Msa0172930:intron 40.0%
! TAGGGAAAGTGACTTGTGTT+TGG - chr1_4:58119460-58119479 Msa0172930:intron 40.0%
GGCTAAGAGTGAATTGAAGA+TGG + chr1_4:58119632-58119651 None:intergenic 40.0%
GTTTGGGTGGTATTCAAAAC+TGG - chr1_4:58119733-58119752 Msa0172930:intron 40.0%
!!! ACAAAAGCCTTTTGAAGCAG+GGG + chr1_4:58120007-58120026 None:intergenic 40.0%
!!! CAACAAAAGCCTTTTGAAGC+AGG + chr1_4:58120009-58120028 None:intergenic 40.0%
ATCAGTCTGTCTTCAACATC+TGG - chr1_4:58120084-58120103 Msa0172930:intron 40.0%
GGTCAAATACTTGAGATACC+TGG + chr1_4:58120181-58120200 None:intergenic 40.0%
!! GTTAGCTTCTCTTTTGGCTA+TGG - chr1_4:58120377-58120396 Msa0172930:intron 40.0%
! CGGTAACACTTTTGTTGGAA+TGG - chr1_4:58120467-58120486 Msa0172930:five_prime_UTR 40.0%
AAGGGATTAGGGTATAGGTT+TGG + chr1_4:58118844-58118863 None:intergenic 40.0%
AGAACACAGCACAATGTGAA+GGG + chr1_4:58118889-58118908 None:intergenic 40.0%
TGTTCTGTTCTGTTCTCTTG+TGG - chr1_4:58118908-58118927 Msa0172930:three_prime_UTR 40.0%
TTCTGTTCTCTTGTGGCTAA+AGG - chr1_4:58118915-58118934 Msa0172930:three_prime_UTR 40.0%
TGGTTCAAAGGGAAAGAAGA+AGG - chr1_4:58119061-58119080 Msa0172930:CDS 40.0%
GGTTCAAAGGGAAAGAAGAA+GGG - chr1_4:58119062-58119081 Msa0172930:CDS 40.0%
CTTCAAGAGAGGATTAAGGT+TGG - chr1_4:58119161-58119180 Msa0172930:CDS 40.0%
!! ATTAAGGTTGGTGGTAAACC+TGG - chr1_4:58119173-58119192 Msa0172930:CDS 40.0%
TGATGCTGTTACTGTTTCCA+GGG - chr1_4:58119205-58119224 Msa0172930:intron 40.0%
CTGATAGCAACTTCTCCAAA+CGG - chr1_4:58119252-58119271 Msa0172930:intron 40.0%
! TAGGGAAAGTGACTTGTGTT+TGG - chr1_4:58119460-58119479 Msa0172930:intron 40.0%
GGCTAAGAGTGAATTGAAGA+TGG + chr1_4:58119632-58119651 None:intergenic 40.0%
GTTTGGGTGGTATTCAAAAC+TGG - chr1_4:58119733-58119752 Msa0172930:intron 40.0%
!!! ACAAAAGCCTTTTGAAGCAG+GGG + chr1_4:58120007-58120026 None:intergenic 40.0%
!!! CAACAAAAGCCTTTTGAAGC+AGG + chr1_4:58120009-58120028 None:intergenic 40.0%
ATCAGTCTGTCTTCAACATC+TGG - chr1_4:58120084-58120103 Msa0172930:intron 40.0%
GGTCAAATACTTGAGATACC+TGG + chr1_4:58120181-58120200 None:intergenic 40.0%
!! GTTAGCTTCTCTTTTGGCTA+TGG - chr1_4:58120377-58120396 Msa0172930:intron 40.0%
! CGGTAACACTTTTGTTGGAA+TGG - chr1_4:58120467-58120486 Msa0172930:five_prime_UTR 40.0%
CAGAACACAGCACAATGTGA+AGG + chr1_4:58118890-58118909 None:intergenic 45.0%
! AGTAGATGCAGCTGGTTCAA+AGG - chr1_4:58119049-58119068 Msa0172930:exon 45.0%
! GTAGATGCAGCTGGTTCAAA+GGG - chr1_4:58119050-58119069 Msa0172930:exon 45.0%
TCCATGATCTTGTCTTCCAC+GGG + chr1_4:58119119-58119138 None:intergenic 45.0%
GTCCATGATCTTGTCTTCCA+CGG + chr1_4:58119120-58119139 None:intergenic 45.0%
CAAGAGAGGATTAAGGTTGG+TGG - chr1_4:58119164-58119183 Msa0172930:CDS 45.0%
GTGATGCTGTTACTGTTTCC+AGG - chr1_4:58119204-58119223 Msa0172930:intron 45.0%
CATTGCTGAGAATGAAGGAG+AGG - chr1_4:58120293-58120312 Msa0172930:CDS 45.0%
! ATGCTCGAGACATGTTGCTT+TGG - chr1_4:58120401-58120420 Msa0172930:intron 45.0%
AGCGCCTTCTATACTCTTTC+TGG - chr1_4:58120519-58120538 Msa0172930:five_prime_UTR 45.0%
CAGAACACAGCACAATGTGA+AGG + chr1_4:58118890-58118909 None:intergenic 45.0%
! AGTAGATGCAGCTGGTTCAA+AGG - chr1_4:58119049-58119068 Msa0172930:exon 45.0%
! GTAGATGCAGCTGGTTCAAA+GGG - chr1_4:58119050-58119069 Msa0172930:exon 45.0%
TCCATGATCTTGTCTTCCAC+GGG + chr1_4:58119119-58119138 None:intergenic 45.0%
GTCCATGATCTTGTCTTCCA+CGG + chr1_4:58119120-58119139 None:intergenic 45.0%
CAAGAGAGGATTAAGGTTGG+TGG - chr1_4:58119164-58119183 Msa0172930:CDS 45.0%
GTGATGCTGTTACTGTTTCC+AGG - chr1_4:58119204-58119223 Msa0172930:intron 45.0%
CATTGCTGAGAATGAAGGAG+AGG - chr1_4:58120293-58120312 Msa0172930:CDS 45.0%
! ATGCTCGAGACATGTTGCTT+TGG - chr1_4:58120401-58120420 Msa0172930:intron 45.0%
AGCGCCTTCTATACTCTTTC+TGG - chr1_4:58120519-58120538 Msa0172930:five_prime_UTR 45.0%
AAAACGCGCGTTTCACTTCG+TGG + chr1_4:58118814-58118833 None:intergenic 50.0%
GGCAGAAGGGATTAGGGTAT+AGG + chr1_4:58118849-58118868 None:intergenic 50.0%
AGGGAAAAAATGGACGGCAG+TGG + chr1_4:58118870-58118889 None:intergenic 50.0%
GATCTTGTCTTCCACGGGTT+TGG + chr1_4:58119114-58119133 None:intergenic 50.0%
ACCCGTGGAAGACAAGATCA+TGG - chr1_4:58119115-58119134 Msa0172930:CDS 50.0%
AAAACGCGCGTTTCACTTCG+TGG + chr1_4:58118814-58118833 None:intergenic 50.0%
GGCAGAAGGGATTAGGGTAT+AGG + chr1_4:58118849-58118868 None:intergenic 50.0%
AGGGAAAAAATGGACGGCAG+TGG + chr1_4:58118870-58118889 None:intergenic 50.0%
GATCTTGTCTTCCACGGGTT+TGG + chr1_4:58119114-58119133 None:intergenic 50.0%
ACCCGTGGAAGACAAGATCA+TGG - chr1_4:58119115-58119134 Msa0172930:CDS 50.0%
GGCAGTGGCAGAAGGGATTA+GGG + chr1_4:58118855-58118874 None:intergenic 55.0%
AATGGACGGCAGTGGCAGAA+GGG + chr1_4:58118862-58118881 None:intergenic 55.0%
AAATGGACGGCAGTGGCAGA+AGG + chr1_4:58118863-58118882 None:intergenic 55.0%
ATGGGTCGAGTAGATGCAGC+TGG - chr1_4:58119041-58119060 Msa0172930:exon 55.0%
CACGGGTTTGGCACAGTCAA+TGG + chr1_4:58119102-58119121 None:intergenic 55.0%
! CATTGACTGTGCCAAACCCG+TGG - chr1_4:58119100-58119119 Msa0172930:CDS 55.0%
GGTGGTAAACCTGGTGCTCT+TGG - chr1_4:58119182-58119201 Msa0172930:intron 55.0%
!! ACAGCATCACCAAGAGCACC+AGG + chr1_4:58119194-58119213 None:intergenic 55.0%
GGCAGTGGCAGAAGGGATTA+GGG + chr1_4:58118855-58118874 None:intergenic 55.0%
AATGGACGGCAGTGGCAGAA+GGG + chr1_4:58118862-58118881 None:intergenic 55.0%
AAATGGACGGCAGTGGCAGA+AGG + chr1_4:58118863-58118882 None:intergenic 55.0%
ATGGGTCGAGTAGATGCAGC+TGG - chr1_4:58119041-58119060 Msa0172930:exon 55.0%
CACGGGTTTGGCACAGTCAA+TGG + chr1_4:58119102-58119121 None:intergenic 55.0%
! CATTGACTGTGCCAAACCCG+TGG - chr1_4:58119100-58119119 Msa0172930:CDS 55.0%
GGTGGTAAACCTGGTGCTCT+TGG - chr1_4:58119182-58119201 Msa0172930:intron 55.0%
!! ACAGCATCACCAAGAGCACC+AGG + chr1_4:58119194-58119213 None:intergenic 55.0%
CGGCAGTGGCAGAAGGGATT+AGG + chr1_4:58118856-58118875 None:intergenic 60.0%
CGGCAGTGGCAGAAGGGATT+AGG + chr1_4:58118856-58118875 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 58118794 58120587 58118794 ID=Msa0172930;Name=Msa0172930
chr1_4 mRNA 58118794 58120587 58118794 ID=Msa0172930-mRNA-1;Parent=Msa0172930;Name=Msa0172930-mRNA-1;_AED=0.00;_eAED=0.00;_QI=152|1|1|1|0.5|0.33|3|261|125
chr1_4 exon 58118794 58119199 58118794 ID=Msa0172930-mRNA-1:exon:17896;Parent=Msa0172930-mRNA-1
chr1_4 exon 58120108 58120349 58120108 ID=Msa0172930-mRNA-1:exon:17895;Parent=Msa0172930-mRNA-1
chr1_4 exon 58120445 58120587 58120445 ID=Msa0172930-mRNA-1:exon:17894;Parent=Msa0172930-mRNA-1
chr1_4 five_prime_UTR 58120445 58120587 58120445 ID=Msa0172930-mRNA-1:five_prime_utr;Parent=Msa0172930-mRNA-1
chr1_4 five_prime_UTR 58120341 58120349 58120341 ID=Msa0172930-mRNA-1:five_prime_utr;Parent=Msa0172930-mRNA-1
chr1_4 CDS 58120108 58120340 58120108 ID=Msa0172930-mRNA-1:cds;Parent=Msa0172930-mRNA-1
chr1_4 CDS 58119055 58119199 58119055 ID=Msa0172930-mRNA-1:cds;Parent=Msa0172930-mRNA-1
chr1_4 three_prime_UTR 58118794 58119054 58118794 ID=Msa0172930-mRNA-1:three_prime_utr;Parent=Msa0172930-mRNA-1
Gene Sequence

>Msa0172930

ATGGGTCGAGTAGATGCAGCTGGTTCAAAGGGAAAGAAGAAGGGAGTGATAACATTCACCATTGACTGTGCCAAACCCGTGGAAGACAAGATCATGGACATCGCTTCTCTCGAAAAGTTTCTTCAAGAGAGGATTAAGGTTGGTGGTAAACCTGGTGCTCTTGGTGATGCTGTTACTGTTTCCAGGGAAAAAAGCAAGATTACTGTTACTTCTGATAGCAACTTCTCCAAACGGTATCTCAAGTATTTGACCAAAAAGTACTTGAAGAAACACAATGTAAGGGATTGGCTCAGAGTGATTGCTTCAAACAAAGATAGAAATGTTTATGAGCTGAGGTACTTCAACATTGCTGAGAATGAAGGAGAGGAAGAAGACTGA
Protein sequence

>Msa0172930

MGRVDAAGSKGKKKGVITFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKPGALGDAVTVSREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRNVYELRYFNIAENEGEEED*