AlfalfaGEDB | Msa1332250

M. sativa cultivar zhongmu-4 / Msa1332250

KEGG: K02997
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005840 GO:0005911 GO:0006417 GO:0006450 GO:0008150 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0015935 GO:0016020 GO:0019222 GO:0019843 GO:0022626 GO:0022627 GO:0030054 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0032991 GO:0034248 GO:0034250 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045727 GO:0045903 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0055044 GO:0060255 GO:0065007 GO:0065008 GO:0080090 GO:0097159 GO:1901363 GO:1990904 GO:2000112
PFAM: PF00163.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332250	XP_003598026.1	100.000	197	0	0	1	197	1	197	5.17e-142	404

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332250	sp\|Q9FLF0\|RS92_ARATH	90.909	187	17	0	1	187	1	187	2.10e-123	349

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332250	B7FMD4	100.000	197	0	0	1	197	1	197	2.47e-142	404

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002750	Msa1332250	0.833938	3.999745e-56	-8.615850e-47
Msa0002790	Msa1332250	0.840805	6.970659e-58	-8.615850e-47
Msa0011740	Msa1332250	0.824032	1.005184e-53	-8.615850e-47
Msa0022970	Msa1332250	0.811477	6.865930e-51	-8.615850e-47
Msa0032780	Msa1332250	0.877406	6.448796e-69	-8.615850e-47
Msa0032810	Msa1332250	0.821444	4.024110e-53	-8.615850e-47
Msa0040220	Msa1332250	0.804525	2.072072e-49	-8.615850e-47
Msa0042480	Msa1332250	0.832070	1.165624e-55	-8.615850e-47
Msa0044890	Msa1332250	0.842974	1.862389e-58	-8.615850e-47
Msa0045730	Msa1332250	0.811981	5.334493e-51	-8.615850e-47
Msa0049690	Msa1332250	0.813379	2.637533e-51	-8.615850e-47
Msa0068970	Msa1332250	0.840540	8.177244e-58	-8.615850e-47
Msa0074030	Msa1332250	0.849863	2.461177e-60	-8.615850e-47
Msa0079590	Msa1332250	0.825835	3.774730e-54	-8.615850e-47
Msa0085780	Msa1332250	0.820983	5.139732e-53	-8.615850e-47
Msa0086750	Msa1332250	0.801177	1.018235e-48	-8.615850e-47
Msa0087910	Msa1332250	0.816417	5.591554e-52	-8.615850e-47
Msa0091750	Msa1332250	0.824946	6.128459e-54	-8.615850e-47
Msa0114570	Msa1332250	0.838005	3.717984e-57	-8.615850e-47
Msa0116130	Msa1332250	0.841981	3.416634e-58	-8.615850e-47
Msa0116670	Msa1332250	0.894646	2.026677e-75	-8.615850e-47
Msa0615580	Msa1332250	0.817328	3.490434e-52	-8.615850e-47
Msa0617540	Msa1332250	0.835321	1.796232e-56	-8.615850e-47
Msa0622750	Msa1332250	0.825723	4.013132e-54	-8.615850e-47
Msa0623200	Msa1332250	0.815827	7.572109e-52	-8.615850e-47
Msa0625570	Msa1332250	0.809932	1.482079e-50	-8.615850e-47
Msa0631250	Msa1332250	0.808264	3.373229e-50	-8.615850e-47
Msa0653060	Msa1332250	0.808060	3.728274e-50	-8.615850e-47
Msa0656410	Msa1332250	0.826219	3.058654e-54	-8.615850e-47
Msa0664560	Msa1332250	0.823434	1.387925e-53	-8.615850e-47
Msa0698780	Msa1332250	0.823797	1.141490e-53	-8.615850e-47
Msa0705800	Msa1332250	0.847646	1.013730e-59	-8.615850e-47
Msa0711960	Msa1332250	0.803239	3.833358e-49	-8.615850e-47
Msa0712610	Msa1332250	0.848679	5.256748e-60	-8.615850e-47
Msa0717380	Msa1332250	0.875194	3.733548e-68	-8.615850e-47
Msa0729200	Msa1332250	0.844929	5.573830e-59	-8.615850e-47
Msa0752910	Msa1332250	0.815151	1.070866e-51	-8.615850e-47
Msa0766080	Msa1332250	0.823424	1.395473e-53	-8.615850e-47
Msa0769590	Msa1332250	0.840499	8.383587e-58	-8.615850e-47
Msa0781080	Msa1332250	0.848379	6.363780e-60	-8.615850e-47
Msa0781310	Msa1332250	0.832609	8.572031e-56	-8.615850e-47
Msa0783630	Msa1332250	0.846043	2.783031e-59	-8.615850e-47
Msa0785130	Msa1332250	0.830012	3.730812e-55	-8.615850e-47
Msa0786660	Msa1332250	0.809604	1.742968e-50	-8.615850e-47
Msa0788380	Msa1332250	0.818216	2.200365e-52	-8.615850e-47
Msa0792420	Msa1332250	0.831015	2.120930e-55	-8.615850e-47
Msa0799830	Msa1332250	0.853042	3.104256e-61	-8.615850e-47
Msa0806880	Msa1332250	0.829002	6.566169e-55	-8.615850e-47
Msa0817540	Msa1332250	0.819292	1.253970e-52	-8.615850e-47
Msa0822580	Msa1332250	0.824708	6.974163e-54	-8.615850e-47
Msa0827380	Msa1332250	0.805552	1.263110e-49	-8.615850e-47
Msa0838070	Msa1332250	0.834167	3.504498e-56	-8.615850e-47
Msa0129660	Msa1332250	0.835700	1.440203e-56	-8.615850e-47
Msa0130420	Msa1332250	0.834176	3.486712e-56	-8.615850e-47
Msa0133540	Msa1332250	0.836325	9.999406e-57	-8.615850e-47
Msa0138890	Msa1332250	0.804734	1.874037e-49	-8.615850e-47
Msa0140850	Msa1332250	0.824564	7.539904e-54	-8.615850e-47
Msa0172900	Msa1332250	0.855198	7.411174e-62	-8.615850e-47
Msa0172930	Msa1332250	0.862248	5.796281e-64	-8.615850e-47
Msa0173530	Msa1332250	0.894751	1.836459e-75	-8.615850e-47
Msa0181490	Msa1332250	0.826145	3.186177e-54	-8.615850e-47
Msa0183090	Msa1332250	0.837022	6.640290e-57	-8.615850e-47
Msa0186740	Msa1332250	0.807158	5.793979e-50	-8.615850e-47
Msa0189700	Msa1332250	0.842481	2.518845e-58	-8.615850e-47
Msa0191450	Msa1332250	0.814749	1.314793e-51	-8.615850e-47
Msa0191550	Msa1332250	0.811312	7.456308e-51	-8.615850e-47
Msa0192230	Msa1332250	0.825094	5.652717e-54	-8.615850e-47
Msa0194940	Msa1332250	0.868474	6.358645e-66	-8.615850e-47
Msa0210330	Msa1332250	0.836538	8.825594e-57	-8.615850e-47
Msa0210360	Msa1332250	0.896526	3.391559e-76	-8.615850e-47
Msa0218720	Msa1332250	0.805961	1.036887e-49	-8.615850e-47
Msa0218830	Msa1332250	0.868563	5.949621e-66	-8.615850e-47
Msa0219120	Msa1332250	0.837783	4.239122e-57	-8.615850e-47
Msa0221030	Msa1332250	0.836860	7.304940e-57	-8.615850e-47
Msa0222730	Msa1332250	0.852104	5.743822e-61	-8.615850e-47
Msa0229150	Msa1332250	0.814768	1.302181e-51	-8.615850e-47
Msa0230490	Msa1332250	0.870242	1.691988e-66	-8.615850e-47
Msa0232010	Msa1332250	0.828730	7.641155e-55	-8.615850e-47
Msa0232080	Msa1332250	0.829559	4.810680e-55	-8.615850e-47
Msa1256840	Msa1332250	0.867528	1.280858e-65	-8.615850e-47
Msa1266060	Msa1332250	0.858787	6.480428e-63	-8.615850e-47
Msa1273340	Msa1332250	0.837167	6.096886e-57	-8.615850e-47
Msa1295150	Msa1332250	0.808896	2.471925e-50	-8.615850e-47
Msa1299590	Msa1332250	0.870947	9.924412e-67	-8.615850e-47
Msa1305050	Msa1332250	0.803715	3.054187e-49	-8.615850e-47
Msa1311400	Msa1332250	0.914087	2.889863e-84	-8.615850e-47
Msa1321790	Msa1332250	0.822720	2.036734e-53	-8.615850e-47
Msa1332250	Msa1338190	0.869344	3.322267e-66	-8.615850e-47
Msa1332250	Msa1343260	0.879272	1.426464e-69	-8.615850e-47
Msa1332250	Msa1349570	0.821320	4.299771e-53	-8.615850e-47
Msa1332250	Msa1356790	0.845291	4.451287e-59	-8.615850e-47
Msa1332250	Msa1362210	0.805535	1.273914e-49	-8.615850e-47
Msa1332250	Msa1362790	0.865505	5.624626e-65	-8.615850e-47
Msa1332250	Msa1368920	0.874694	5.527518e-68	-8.615850e-47
Msa1332250	Msa1369190	0.820213	7.726056e-53	-8.615850e-47
Msa1332250	Msa1374240	0.836548	8.774961e-57	-8.615850e-47
Msa1332250	Msa1392590	0.828982	6.640390e-55	-8.615850e-47
Msa1332250	Msa1393000	0.816417	5.591554e-52	-8.615850e-47
Msa1332250	Msa1397100	0.856202	3.774320e-62	-8.615850e-47
Msa1332250	Msa1403910	0.848081	7.694603e-60	-8.615850e-47
Msa1332250	Msa1406080	0.806803	6.888278e-50	-8.615850e-47
Msa1332250	Msa1410440	0.853472	2.336078e-61	-8.615850e-47
Msa1332250	Msa1413500	0.825718	4.024189e-54	-8.615850e-47
Msa1332250	Msa1418770	0.911427	6.154117e-83	-8.615850e-47
Msa1332250	Msa1426120	0.932954	3.913042e-95	-8.615850e-47
Msa1332250	Msa1426370	0.839892	1.207599e-57	-8.615850e-47
Msa1332250	Msa1426490	0.856780	2.553037e-62	-8.615850e-47
Msa1332250	Msa1437280	0.818560	1.839097e-52	-8.615850e-47
Msa1332250	Msa1446410	0.803753	2.999522e-49	-8.615850e-47
Msa0426090	Msa1332250	0.810869	9.302733e-51	-8.615850e-47
Msa0426250	Msa1332250	0.823846	1.111632e-53	-8.615850e-47
Msa0443390	Msa1332250	0.837315	5.589907e-57	-8.615850e-47
Msa0463520	Msa1332250	0.816526	5.284459e-52	-8.615850e-47
Msa0471880	Msa1332250	0.855317	6.840264e-62	-8.615850e-47
Msa0475810	Msa1332250	0.821258	4.443033e-53	-8.615850e-47
Msa0481520	Msa1332250	0.821216	4.542162e-53	-8.615850e-47
Msa0481850	Msa1332250	0.838142	3.427542e-57	-8.615850e-47
Msa1019240	Msa1332250	0.814734	1.324968e-51	-8.615850e-47
Msa1019260	Msa1332250	0.818535	1.863445e-52	-8.615850e-47
Msa1024180	Msa1332250	0.831330	1.774839e-55	-8.615850e-47
Msa1037290	Msa1332250	0.876841	1.012612e-68	-8.615850e-47
Msa1039520	Msa1332250	0.848729	5.090380e-60	-8.615850e-47
Msa1040160	Msa1332250	0.843933	1.032878e-58	-8.615850e-47
Msa1078870	Msa1332250	0.929147	1.054501e-92	-8.615850e-47
Msa1078980	Msa1332250	0.901808	1.842275e-78	-8.615850e-47
Msa1080350	Msa1332250	0.853536	2.238934e-61	-8.615850e-47
Msa1082170	Msa1332250	0.829425	5.183844e-55	-8.615850e-47
Msa1083370	Msa1332250	0.815910	7.255622e-52	-8.615850e-47
Msa1088490	Msa1332250	0.803306	3.713292e-49	-8.615850e-47
Msa1089220	Msa1332250	0.855521	5.965311e-62	-8.615850e-47
Msa1094380	Msa1332250	0.837951	3.839139e-57	-8.615850e-47
Msa1104210	Msa1332250	0.810483	1.127028e-50	-8.615850e-47
Msa0250820	Msa1332250	0.823589	1.277085e-53	-8.615850e-47
Msa0250870	Msa1332250	0.809126	2.207816e-50	-8.615850e-47
Msa0257450	Msa1332250	0.825862	3.717990e-54	-8.615850e-47
Msa0258850	Msa1332250	0.836347	9.867543e-57	-8.615850e-47
Msa0264010	Msa1332250	0.836460	9.240396e-57	-8.615850e-47
Msa0265250	Msa1332250	0.847680	9.921225e-60	-8.615850e-47
Msa0289580	Msa1332250	0.835006	2.156777e-56	-8.615850e-47
Msa0289610	Msa1332250	0.837676	4.516046e-57	-8.615850e-47
Msa0297830	Msa1332250	0.869760	2.432641e-66	-8.615850e-47
Msa0298200	Msa1332250	0.831852	1.319397e-55	-8.615850e-47
Msa0300350	Msa1332250	0.801477	8.840462e-49	-8.615850e-47
Msa0301220	Msa1332250	0.808380	3.186694e-50	-8.615850e-47
Msa0306690	Msa1332250	0.820713	5.932323e-53	-8.615850e-47
Msa0327300	Msa1332250	0.821165	4.668373e-53	-8.615850e-47
Msa0335840	Msa1332250	0.880069	7.431641e-70	-8.615850e-47
Msa0337670	Msa1332250	0.811569	6.557236e-51	-8.615850e-47
Msa0342850	Msa1332250	0.831499	1.612962e-55	-8.615850e-47
Msa0348830	Msa1332250	0.851907	6.536706e-61	-8.615850e-47
Msa1120790	Msa1332250	0.805144	1.537970e-49	-8.615850e-47
Msa1125860	Msa1332250	0.844709	6.391237e-59	-8.615850e-47
Msa1126060	Msa1332250	0.840480	8.479538e-58	-8.615850e-47
Msa1126520	Msa1332250	0.842680	2.230390e-58	-8.615850e-47
Msa1128120	Msa1332250	0.814530	1.470205e-51	-8.615850e-47
Msa1152120	Msa1332250	0.813968	1.956381e-51	-8.615850e-47
Msa1164150	Msa1332250	0.856281	3.577497e-62	-8.615850e-47
Msa1165640	Msa1332250	0.801711	7.914765e-49	-8.615850e-47
Msa1167540	Msa1332250	0.859855	3.097885e-63	-8.615850e-47
Msa1169180	Msa1332250	0.898840	3.574143e-77	-8.615850e-47
Msa1183450	Msa1332250	0.820025	8.529566e-53	-8.615850e-47
Msa1203560	Msa1332250	0.834000	3.858934e-56	-8.615850e-47
Msa1211770	Msa1332250	0.808376	3.192613e-50	-8.615850e-47
Msa1215820	Msa1332250	0.837952	3.835679e-57	-8.615850e-47
Msa1218170	Msa1332250	0.817806	2.724525e-52	-8.615850e-47
Msa0483110	Msa1332250	0.824742	6.846635e-54	-8.615850e-47
Msa0486070	Msa1332250	0.840583	7.968906e-58	-8.615850e-47
Msa0529900	Msa1332250	0.839269	1.752574e-57	-8.615850e-47
Msa0534750	Msa1332250	0.802994	4.306473e-49	-8.615850e-47
Msa0550420	Msa1332250	0.817451	3.276224e-52	-8.615850e-47
Msa0584030	Msa1332250	0.883718	3.538859e-71	-8.615850e-47
Msa0584260	Msa1332250	0.817651	2.951861e-52	-8.615850e-47
Msa0586420	Msa1332250	0.815218	1.034896e-51	-8.615850e-47
Msa0588360	Msa1332250	0.817743	2.815157e-52	-8.615850e-47
Msa0590940	Msa1332250	0.801769	7.701016e-49	-8.615850e-47
Msa0860860	Msa1332250	0.811148	8.091703e-51	-8.615850e-47
Msa0861320	Msa1332250	0.832199	1.083409e-55	-8.615850e-47
Msa0867060	Msa1332250	0.857123	2.021724e-62	-8.615850e-47
Msa0876170	Msa1332250	0.821156	4.690265e-53	-8.615850e-47
Msa0881290	Msa1332250	0.842035	3.305830e-58	-8.615850e-47
Msa0888810	Msa1332250	0.841596	4.318509e-58	-8.615850e-47
Msa0915540	Msa1332250	0.830156	3.441710e-55	-8.615850e-47
Msa0945480	Msa1332250	0.825137	5.522545e-54	-8.615850e-47
Msa0953310	Msa1332250	0.825614	4.257615e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332250	MtrunA17_Chr3g0077371	100.000	197	0	1	197	1	197	4.76e-146	404
Msa1332250	MtrunA17_Chr3g0077391	100.000	197	0	1	197	1	197	4.76e-146	404
Msa1332250	MtrunA17_Chr4g0009481	100.000	197	0	1	197	1	197	4.76e-146	404
Msa1332250	MtrunA17_Chr7g0241891	98.985	197	2	1	197	1	197	2.61e-144	399
Msa1332250	MtrunA17_Chr1g0182531	98.477	197	3	1	197	28	224	3.76e-143	397
Msa1332250	MtrunA17_Chr4g0060551	98.261	115	2	1	115	1	115	5.75e-79	231
Msa1332250	MtrunA17_Chr4g0009501	100.000	60	0	138	197	13	72	4.70e-38	126

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1332250	AT5G39850.1	90.909	187	17	0	1	187	1	187	2.13e-124	349
Msa1332250	AT5G15200.1	84.343	198	30	1	1	197	1	198	9.63e-118	332
Msa1332250	AT5G15200.2	89.051	137	15	0	1	137	1	137	4.16e-86	251

Find 83 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACACACAGCATCAAGTAAA+AGG	0.265429	8_4:+52155810	None:intergenic
AATAAACAAGGATGGGAAAA+TGG	0.279207	8_4:+52155934	None:intergenic
TGATCCAACTATGCTTCTTA+TGG	0.287832	8_4:-52155719	Msa1332250:three_prime_UTR
CCTTTCGGTGGTGGTCTTCC+TGG	0.299249	8_4:-52156124	Msa1332250:CDS
ATCACCACCGGAAGCCTTCT+TGG	0.302286	8_4:+52156069	None:intergenic
TTGAAGCTGGTCGGAGAGTA+TGG	0.314070	8_4:-52157725	Msa1332250:CDS
GTTTCATAGGGTTGGGAGGC+AGG	0.324677	8_4:-52156215	Msa1332250:intron
GAACAAGCTCGATTATGTTT+TGG	0.327341	8_4:-52157537	Msa1332250:CDS
TTCGCTGCAAGCGCGAGCTT+TGG	0.327479	8_4:-52157700	Msa1332250:CDS
CAGACACTCGTCTTCAAATC+TGG	0.346433	8_4:-52157479	Msa1332250:CDS
ACATCCCATCCTTCATGGTT+AGG	0.353382	8_4:-52156186	Msa1332250:CDS
CCTTCTTGGCTGCAGCCTTA+AGG	0.354248	8_4:+52156083	None:intergenic
GCTGCAAGCGCGAGCTTTGG+AGG	0.363662	8_4:-52157697	Msa1332250:CDS
ACCATGCTAGGGTTCTTATC+AGG	0.369101	8_4:-52157439	Msa1332250:CDS
CCTCTCCTTGCCGCTGCCGC+CGG	0.390241	8_4:+52157854	None:intergenic
CAAAGTTTCGGTTGGATACA+TGG	0.398736	8_4:+52155740	None:intergenic
GCATAGTTGGATCAAAGTTT+CGG	0.405107	8_4:+52155728	None:intergenic
TGATCTTTATACAAGTTAGT+TGG	0.405273	8_4:-52155635	Msa1332250:three_prime_UTR
ACAAAGATCATCAAATGTAT+TGG	0.418707	8_4:+52155843	None:intergenic
TCCAAACGCTCCTTCTCATA+TGG	0.423037	8_4:+52157755	None:intergenic
GGTGAACATCCCATCCTTCA+TGG	0.426151	8_4:-52156191	Msa1332250:CDS
TCCATATGAGAAGGAGCGTT+TGG	0.433604	8_4:-52157756	Msa1332250:CDS
CATCCCATCCTTCATGGTTA+GGG	0.434949	8_4:-52156185	Msa1332250:CDS
TGCCGCCGGTGATGCCTACT+AGG	0.436069	8_4:+52157868	None:intergenic
GCTGCAGCCAAGAAGGCTTC+CGG	0.439232	8_4:-52156076	Msa1332250:CDS
TTGGATCAAAACTTAATACT+TGG	0.442599	8_4:+52155862	None:intergenic
CTGCAAGCGCGAGCTTTGGA+GGG	0.446963	8_4:-52157696	Msa1332250:CDS
TGTTCTGTGTCTCATCTAGA+AGG	0.448082	8_4:+52157555	None:intergenic
CTCACAAGTCCTTTCGGTGG+TGG	0.464545	8_4:-52156133	Msa1332250:CDS
TTATCAGGCAGAGGCACATC+AGG	0.476066	8_4:-52157424	Msa1332250:intron
TTCTTTCGCTTCACACGACC+AGG	0.480028	8_4:+52156106	None:intergenic
AAAGCGTTCTAGCATTGTTA+CGG	0.481953	8_4:+52157651	None:intergenic
GTAGGCATCACCGGCGGCAG+CGG	0.492678	8_4:-52157864	Msa1332250:five_prime_UTR
TTCTAGCATTGTTACGGATA+CGG	0.498419	8_4:+52157657	None:intergenic
CTGTGAATCAGAGATCAAAA+TGG	0.499446	8_4:-52157831	Msa1332250:exon
GAATCCTCGTCGGATCTTCG+AGG	0.499570	8_4:-52157612	Msa1332250:CDS
TACACCATAAGAAGCATAGT+TGG	0.501489	8_4:+52155715	None:intergenic
AGTTGGATCAAAGTTTCGGT+TGG	0.502951	8_4:+52155732	None:intergenic
AGTCTAGGACAGATATAAAA+CGG	0.515542	8_4:+52155987	None:intergenic
CCTTAAGGCTGCAGCCAAGA+AGG	0.517095	8_4:-52156083	Msa1332250:CDS
CCATCCTTGTTTATTGTGTG+AGG	0.520499	8_4:-52155926	Msa1332250:three_prime_UTR
CTAAGTCCATTCACCATGCT+AGG	0.521010	8_4:-52157451	Msa1332250:CDS
CCGGCGGCAGCGGCAAGGAG+AGG	0.526000	8_4:-52157854	Msa1332250:five_prime_UTR
AAGAAGGCTTCCGGTGGTGA+TGG	0.531981	8_4:-52156067	Msa1332250:CDS
TCATTTGTGATTAAAATGTC+AGG	0.535564	8_4:+52155584	None:intergenic
CATCACCGGCGGCAGCGGCA+AGG	0.537265	8_4:-52157859	Msa1332250:five_prime_UTR
AAGAACCCTAGCATGGTGAA+TGG	0.539163	8_4:+52157445	None:intergenic
CTCCTCATCACCATCACCAC+CGG	0.544543	8_4:+52156057	None:intergenic
TCAACCCTAACCATGAAGGA+TGG	0.545370	8_4:+52156181	None:intergenic
CCTCACACAATAAACAAGGA+TGG	0.552644	8_4:+52155926	None:intergenic
TGTGAAGCGAAAGAACCTTA+AGG	0.553464	8_4:-52156098	Msa1332250:CDS
TGATAGAAAATCTAAAGTCT+AGG	0.553926	8_4:+52155972	None:intergenic
TCATAGGGTTGGGAGGCAGG+TGG	0.557165	8_4:-52156212	Msa1332250:intron
CTCACACAATAAACAAGGAT+GGG	0.565131	8_4:+52155927	None:intergenic
TTATCAGTTCTGAAAGGCAC+AGG	0.567178	8_4:+52155685	None:intergenic
AACTATGCTTCTTATGGTGT+AGG	0.569156	8_4:-52155713	Msa1332250:three_prime_UTR
TGAATCAACCCTAACCATGA+AGG	0.569890	8_4:+52156177	None:intergenic
CTAGGGTTCTTATCAGGCAG+AGG	0.571814	8_4:-52157433	Msa1332250:CDS
CAACCCTAACCATGAAGGAT+GGG	0.574613	8_4:+52156182	None:intergenic
TCACAATTATCAGTTCTGAA+AGG	0.576272	8_4:+52155679	None:intergenic
TTTGGATGCTGAGTTGAAGC+TGG	0.579570	8_4:-52157738	Msa1332250:CDS
TATTGTTTCATAGGGTTGGG+AGG	0.586042	8_4:-52156219	Msa1332250:intron
TTCCGGTGGTGATGGTGATG+AGG	0.588312	8_4:-52156059	Msa1332250:CDS
GATATAAAACGGCTAGAGCA+AGG	0.589602	8_4:+52155998	None:intergenic
AATCCTCGTCGGATCTTCGA+GGG	0.591722	8_4:-52157611	Msa1332250:CDS
AAAGTTTCGGTTGGATACAT+GGG	0.591867	8_4:+52155741	None:intergenic
TTGAGGAGGATGTTTAAGCA+TGG	0.593185	8_4:-52157578	Msa1332250:CDS
GCCTCGTCGTCCATATGAGA+AGG	0.595822	8_4:-52157765	Msa1332250:CDS
TAAGTCCATTCACCATGCTA+GGG	0.602951	8_4:-52157450	Msa1332250:CDS
TCACTCACAAGTCCTTTCGG+TGG	0.620110	8_4:-52156136	Msa1332250:CDS
ACTCGTCTTCAAATCTGGCA+TGG	0.621528	8_4:-52157474	Msa1332250:CDS
GCAGCCAAGAAGGCTTCCGG+TGG	0.625260	8_4:-52156073	Msa1332250:CDS
AACTTGTATAAAGATCAAGC+AGG	0.637230	8_4:+52155641	None:intergenic
GATGCTGAGTTGAAGCTGGT+CGG	0.640820	8_4:-52157734	Msa1332250:CDS
CCAGGAAGACCACCACCGAA+AGG	0.644057	8_4:+52156124	None:intergenic
CGGTGGTGATGGTGATGAGG+AGG	0.644753	8_4:-52156056	Msa1332250:CDS
GCCTGATAAGAACCCTAGCA+TGG	0.650504	8_4:+52157438	None:intergenic
ATTTCCTCACACAATAAACA+AGG	0.669010	8_4:+52155922	None:intergenic
TTTGTGATTAAAATGTCAGG+TGG	0.686722	8_4:+52155587	None:intergenic
TCCTTCTCATATGGACGACG+AGG	0.694526	8_4:+52157764	None:intergenic
TGGATGAGAAGAATCCTCGT+CGG	0.703960	8_4:-52157622	Msa1332250:CDS
AACCTAGTAGGCATCACCGG+CGG	0.714986	8_4:-52157870	Msa1332250:five_prime_UTR
TCACCCTCGAAGATCCGACG+AGG	0.762913	8_4:+52157608	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATAATTCAATGTAATTAT+AGG	+	chr8_4:52156371-52156390	None:intergenic	10.0%
!!	AAATAATTCAATGTAATTAT+AGG	+	chr8_4:52156371-52156390	None:intergenic	10.0%
!!!	GAATTAATCTGATTTTTTTA+TGG	-	chr8_4:52156100-52156119	Msa1332250:CDS	15.0%
!!!	TTTTTTTTTTTTTGAACTCT+CGG	+	chr8_4:52156765-52156784	None:intergenic	15.0%
!!	GAGTTCAAAAAAAAAAAAAA+AGG	-	chr8_4:52156766-52156785	Msa1332250:intron	15.0%
!!	AAAAAAAAAGGATAAAATTG+GGG	+	chr8_4:52157158-52157177	None:intergenic	15.0%
!!	GAAAAAAAAAGGATAAAATT+GGG	+	chr8_4:52157159-52157178	None:intergenic	15.0%
!!	AGAAAAAAAAAGGATAAAAT+TGG	+	chr8_4:52157160-52157179	None:intergenic	15.0%
!!	CTAAATAGAAAGAAAAAAAA+AGG	+	chr8_4:52157170-52157189	None:intergenic	15.0%
!!	TAGTATTAATGAAATATGTT+TGG	-	chr8_4:52157187-52157206	Msa1332250:intron	15.0%
!!!	GAATTAATCTGATTTTTTTA+TGG	-	chr8_4:52156100-52156119	Msa1332250:CDS	15.0%
!!!	TTTTTTTTTTTTTGAACTCT+CGG	+	chr8_4:52156765-52156784	None:intergenic	15.0%
!!	GAGTTCAAAAAAAAAAAAAA+AGG	-	chr8_4:52156766-52156785	Msa1332250:intron	15.0%
!!	AAAAAAAAAGGATAAAATTG+GGG	+	chr8_4:52157158-52157177	None:intergenic	15.0%
!!	GAAAAAAAAAGGATAAAATT+GGG	+	chr8_4:52157159-52157178	None:intergenic	15.0%
!!	AGAAAAAAAAAGGATAAAAT+TGG	+	chr8_4:52157160-52157179	None:intergenic	15.0%
!!	CTAAATAGAAAGAAAAAAAA+AGG	+	chr8_4:52157170-52157189	None:intergenic	15.0%
!!	TAGTATTAATGAAATATGTT+TGG	-	chr8_4:52157187-52157206	Msa1332250:intron	15.0%
!!	TCAGATTAATTCATCATTAA+CGG	+	chr8_4:52156092-52156111	None:intergenic	20.0%
!!!	ATGTTTTTCAAAATGATACA+GGG	+	chr8_4:52156603-52156622	None:intergenic	20.0%
!!!	AATGTTTTTCAAAATGATAC+AGG	+	chr8_4:52156604-52156623	None:intergenic	20.0%
!!	CAAAACATGTATTGAATTAA+AGG	+	chr8_4:52156842-52156861	None:intergenic	20.0%
!!	GCAAATGAAAAATATAATTG+AGG	+	chr8_4:52157120-52157139	None:intergenic	20.0%
!!	TAATGAAATATGTTTGGATA+TGG	-	chr8_4:52157193-52157212	Msa1332250:intron	20.0%
!!!	AATGTCATTTATTGTTTCAT+AGG	-	chr8_4:52157221-52157240	Msa1332250:intron	20.0%
!!!	ATGTCATTTATTGTTTCATA+GGG	-	chr8_4:52157222-52157241	Msa1332250:intron	20.0%
!!!	TTTGATATTGCATATTTTGA+TGG	-	chr8_4:52157681-52157700	Msa1332250:CDS	20.0%
!!	TCAGATTAATTCATCATTAA+CGG	+	chr8_4:52156092-52156111	None:intergenic	20.0%
!!!	ATGTTTTTCAAAATGATACA+GGG	+	chr8_4:52156603-52156622	None:intergenic	20.0%
!!!	AATGTTTTTCAAAATGATAC+AGG	+	chr8_4:52156604-52156623	None:intergenic	20.0%
!!	CAAAACATGTATTGAATTAA+AGG	+	chr8_4:52156842-52156861	None:intergenic	20.0%
!!	GCAAATGAAAAATATAATTG+AGG	+	chr8_4:52157120-52157139	None:intergenic	20.0%
!!	TAATGAAATATGTTTGGATA+TGG	-	chr8_4:52157193-52157212	Msa1332250:intron	20.0%
!!!	AATGTCATTTATTGTTTCAT+AGG	-	chr8_4:52157221-52157240	Msa1332250:intron	20.0%
!!!	ATGTCATTTATTGTTTCATA+GGG	-	chr8_4:52157222-52157241	Msa1332250:intron	20.0%
!!!	TTTGATATTGCATATTTTGA+TGG	-	chr8_4:52157681-52157700	Msa1332250:CDS	20.0%
!!	CTATAAAGAAAATTAGCTTG+TGG	+	chr8_4:52156282-52156301	None:intergenic	25.0%
!	GGCTTATTTGAACATATATT+TGG	+	chr8_4:52156568-52156587	None:intergenic	25.0%
!!	AAAAGGAAAGTATATCTAGA+AGG	-	chr8_4:52156783-52156802	Msa1332250:intron	25.0%
!	ATTTATTGTTTCATAGGGTT+GGG	-	chr8_4:52157227-52157246	Msa1332250:intron	25.0%
!!	TGATAGAAAATCTAAAGTCT+AGG	+	chr8_4:52157480-52157499	None:intergenic	25.0%
!	TTGGATCAAAACTTAATACT+TGG	+	chr8_4:52157590-52157609	None:intergenic	25.0%
!	ACAAAGATCATCAAATGTAT+TGG	+	chr8_4:52157609-52157628	None:intergenic	25.0%
!	TGATCTTTATACAAGTTAGT+TGG	-	chr8_4:52157814-52157833	Msa1332250:CDS	25.0%
!	TCATTTGTGATTAAAATGTC+AGG	+	chr8_4:52157868-52157887	None:intergenic	25.0%
!!	CTATAAAGAAAATTAGCTTG+TGG	+	chr8_4:52156282-52156301	None:intergenic	25.0%
!	GGCTTATTTGAACATATATT+TGG	+	chr8_4:52156568-52156587	None:intergenic	25.0%
!!	AAAAGGAAAGTATATCTAGA+AGG	-	chr8_4:52156783-52156802	Msa1332250:intron	25.0%
!	ATTTATTGTTTCATAGGGTT+GGG	-	chr8_4:52157227-52157246	Msa1332250:intron	25.0%
!!	TGATAGAAAATCTAAAGTCT+AGG	+	chr8_4:52157480-52157499	None:intergenic	25.0%
!	TTGGATCAAAACTTAATACT+TGG	+	chr8_4:52157590-52157609	None:intergenic	25.0%
!	ACAAAGATCATCAAATGTAT+TGG	+	chr8_4:52157609-52157628	None:intergenic	25.0%
!	TGATCTTTATACAAGTTAGT+TGG	-	chr8_4:52157814-52157833	Msa1332250:CDS	25.0%
!	TCATTTGTGATTAAAATGTC+AGG	+	chr8_4:52157868-52157887	None:intergenic	25.0%
!	TGCTGATCTATTTGTTAGTT+TGG	-	chr8_4:52156146-52156165	Msa1332250:CDS	30.0%
	AGTGCGAAAGCAATTATAAT+AGG	+	chr8_4:52156213-52156232	None:intergenic	30.0%
!!!	TTTTTTTTTTGAACTCTCGG+AGG	+	chr8_4:52156762-52156781	None:intergenic	30.0%
!	ATATCTAGAAGGAAGTATAC+AGG	-	chr8_4:52156794-52156813	Msa1332250:intron	30.0%
	AAAGAGTTACAAGCAGTTAT+GGG	+	chr8_4:52156875-52156894	None:intergenic	30.0%
!	TTTACTTGCATCTTTTGTAG+TGG	-	chr8_4:52156896-52156915	Msa1332250:intron	30.0%
	AAGTAGAGATCATCAAGTAA+TGG	-	chr8_4:52156967-52156986	Msa1332250:intron	30.0%
	ACACTAACAAGTATAGTGTT+AGG	+	chr8_4:52157042-52157061	None:intergenic	30.0%
	TCTATTTACTTACCTCTGAA+TGG	-	chr8_4:52157092-52157111	Msa1332250:intron	30.0%
!!	TTTCATTTGCTGTATGCTTA+TGG	-	chr8_4:52157130-52157149	Msa1332250:intron	30.0%
!!	TTCATTTGCTGTATGCTTAT+GGG	-	chr8_4:52157131-52157150	Msa1332250:intron	30.0%
	CATTTATTGTTTCATAGGGT+TGG	-	chr8_4:52157226-52157245	Msa1332250:intron	30.0%
!	AGTCTAGGACAGATATAAAA+CGG	+	chr8_4:52157465-52157484	None:intergenic	30.0%
	AATAAACAAGGATGGGAAAA+TGG	+	chr8_4:52157518-52157537	None:intergenic	30.0%
	ATTTCCTCACACAATAAACA+AGG	+	chr8_4:52157530-52157549	None:intergenic	30.0%
!!	TCACAATTATCAGTTCTGAA+AGG	+	chr8_4:52157773-52157792	None:intergenic	30.0%
	AACTTGTATAAAGATCAAGC+AGG	+	chr8_4:52157811-52157830	None:intergenic	30.0%
	TTTGTGATTAAAATGTCAGG+TGG	+	chr8_4:52157865-52157884	None:intergenic	30.0%
!	TGCTGATCTATTTGTTAGTT+TGG	-	chr8_4:52156146-52156165	Msa1332250:CDS	30.0%
	AGTGCGAAAGCAATTATAAT+AGG	+	chr8_4:52156213-52156232	None:intergenic	30.0%
!!!	TTTTTTTTTTGAACTCTCGG+AGG	+	chr8_4:52156762-52156781	None:intergenic	30.0%
!	ATATCTAGAAGGAAGTATAC+AGG	-	chr8_4:52156794-52156813	Msa1332250:intron	30.0%
	AAAGAGTTACAAGCAGTTAT+GGG	+	chr8_4:52156875-52156894	None:intergenic	30.0%
!	TTTACTTGCATCTTTTGTAG+TGG	-	chr8_4:52156896-52156915	Msa1332250:intron	30.0%
	AAGTAGAGATCATCAAGTAA+TGG	-	chr8_4:52156967-52156986	Msa1332250:intron	30.0%
	ACACTAACAAGTATAGTGTT+AGG	+	chr8_4:52157042-52157061	None:intergenic	30.0%
	TCTATTTACTTACCTCTGAA+TGG	-	chr8_4:52157092-52157111	Msa1332250:intron	30.0%
!!	TTTCATTTGCTGTATGCTTA+TGG	-	chr8_4:52157130-52157149	Msa1332250:intron	30.0%
!!	TTCATTTGCTGTATGCTTAT+GGG	-	chr8_4:52157131-52157150	Msa1332250:intron	30.0%
	CATTTATTGTTTCATAGGGT+TGG	-	chr8_4:52157226-52157245	Msa1332250:intron	30.0%
!	AGTCTAGGACAGATATAAAA+CGG	+	chr8_4:52157465-52157484	None:intergenic	30.0%
	AATAAACAAGGATGGGAAAA+TGG	+	chr8_4:52157518-52157537	None:intergenic	30.0%
	ATTTCCTCACACAATAAACA+AGG	+	chr8_4:52157530-52157549	None:intergenic	30.0%
!!	TCACAATTATCAGTTCTGAA+AGG	+	chr8_4:52157773-52157792	None:intergenic	30.0%
	AACTTGTATAAAGATCAAGC+AGG	+	chr8_4:52157811-52157830	None:intergenic	30.0%
	TTTGTGATTAAAATGTCAGG+TGG	+	chr8_4:52157865-52157884	None:intergenic	30.0%
	CTGTGAATCAGAGATCAAAA+TGG	-	chr8_4:52155618-52155637	Msa1332250:three_prime_UTR	35.0%
!	GTTGCGTTTTATCGAAACTA+CGG	-	chr8_4:52155646-52155665	Msa1332250:three_prime_UTR	35.0%
	TTCTAGCATTGTTACGGATA+CGG	+	chr8_4:52155795-52155814	None:intergenic	35.0%
!	AAAGCGTTCTAGCATTGTTA+CGG	+	chr8_4:52155801-52155820	None:intergenic	35.0%
!	GAACAAGCTCGATTATGTTT+TGG	-	chr8_4:52155912-52155931	Msa1332250:three_prime_UTR	35.0%
	TATAATTGCTTTCGCACTGT+TGG	-	chr8_4:52156215-52156234	Msa1332250:intron	35.0%
	AATAAGCTGCTTATCCAATC+AGG	+	chr8_4:52156242-52156261	None:intergenic	35.0%
	TATGAAGTACGAAATCTCCT+CGG	-	chr8_4:52156307-52156326	Msa1332250:intron	35.0%
!!	TATAGGTTTAGTGTCGATGT+CGG	+	chr8_4:52156354-52156373	None:intergenic	35.0%
	TCAAATAAGCCAATCCAAAC+AGG	-	chr8_4:52156577-52156596	Msa1332250:intron	35.0%
	TTGTTGTGTGATGTAGATCT+TGG	-	chr8_4:52156654-52156673	Msa1332250:intron	35.0%
	GTAGATCTTGGTTAGATCAT+TGG	-	chr8_4:52156666-52156685	Msa1332250:intron	35.0%
	TTGGTTGTCTGTAACAATGT+TGG	-	chr8_4:52156685-52156704	Msa1332250:intron	35.0%
	TGGTTGTCTGTAACAATGTT+GGG	-	chr8_4:52156686-52156705	Msa1332250:intron	35.0%
	AAGAGTTACAAGCAGTTATG+GGG	+	chr8_4:52156874-52156893	None:intergenic	35.0%
	GAAAGAGTTACAAGCAGTTA+TGG	+	chr8_4:52156876-52156895	None:intergenic	35.0%
	TAGAGATCATCAAGTAATGG+AGG	-	chr8_4:52156970-52156989	Msa1332250:intron	35.0%
	AATTACACATTTCCGAACCA+TGG	+	chr8_4:52157005-52157024	None:intergenic	35.0%
	ATAATTGAGGATCCATTCAG+AGG	+	chr8_4:52157107-52157126	None:intergenic	35.0%
!	TTTTCACTCACAAGTCCTTT+CGG	-	chr8_4:52157310-52157329	Msa1332250:intron	35.0%
	CTCACACAATAAACAAGGAT+GGG	+	chr8_4:52157525-52157544	None:intergenic	35.0%
	TACACACAGCATCAAGTAAA+AGG	+	chr8_4:52157642-52157661	None:intergenic	35.0%
	AAAGTTTCGGTTGGATACAT+GGG	+	chr8_4:52157711-52157730	None:intergenic	35.0%
	GCATAGTTGGATCAAAGTTT+CGG	+	chr8_4:52157724-52157743	None:intergenic	35.0%
	TGATCCAACTATGCTTCTTA+TGG	-	chr8_4:52157730-52157749	Msa1332250:CDS	35.0%
!	TACACCATAAGAAGCATAGT+TGG	+	chr8_4:52157737-52157756	None:intergenic	35.0%
	AACTATGCTTCTTATGGTGT+AGG	-	chr8_4:52157736-52157755	Msa1332250:CDS	35.0%
	CTGTGAATCAGAGATCAAAA+TGG	-	chr8_4:52155618-52155637	Msa1332250:three_prime_UTR	35.0%
!	GTTGCGTTTTATCGAAACTA+CGG	-	chr8_4:52155646-52155665	Msa1332250:three_prime_UTR	35.0%
	TTCTAGCATTGTTACGGATA+CGG	+	chr8_4:52155795-52155814	None:intergenic	35.0%
!	AAAGCGTTCTAGCATTGTTA+CGG	+	chr8_4:52155801-52155820	None:intergenic	35.0%
!	GAACAAGCTCGATTATGTTT+TGG	-	chr8_4:52155912-52155931	Msa1332250:three_prime_UTR	35.0%
	TATAATTGCTTTCGCACTGT+TGG	-	chr8_4:52156215-52156234	Msa1332250:intron	35.0%
	AATAAGCTGCTTATCCAATC+AGG	+	chr8_4:52156242-52156261	None:intergenic	35.0%
	TATGAAGTACGAAATCTCCT+CGG	-	chr8_4:52156307-52156326	Msa1332250:intron	35.0%
!!	TATAGGTTTAGTGTCGATGT+CGG	+	chr8_4:52156354-52156373	None:intergenic	35.0%
	TCAAATAAGCCAATCCAAAC+AGG	-	chr8_4:52156577-52156596	Msa1332250:intron	35.0%
	TTGTTGTGTGATGTAGATCT+TGG	-	chr8_4:52156654-52156673	Msa1332250:intron	35.0%
	GTAGATCTTGGTTAGATCAT+TGG	-	chr8_4:52156666-52156685	Msa1332250:intron	35.0%
	TTGGTTGTCTGTAACAATGT+TGG	-	chr8_4:52156685-52156704	Msa1332250:intron	35.0%
	TGGTTGTCTGTAACAATGTT+GGG	-	chr8_4:52156686-52156705	Msa1332250:intron	35.0%
	AAGAGTTACAAGCAGTTATG+GGG	+	chr8_4:52156874-52156893	None:intergenic	35.0%
	GAAAGAGTTACAAGCAGTTA+TGG	+	chr8_4:52156876-52156895	None:intergenic	35.0%
	TAGAGATCATCAAGTAATGG+AGG	-	chr8_4:52156970-52156989	Msa1332250:intron	35.0%
	AATTACACATTTCCGAACCA+TGG	+	chr8_4:52157005-52157024	None:intergenic	35.0%
	ATAATTGAGGATCCATTCAG+AGG	+	chr8_4:52157107-52157126	None:intergenic	35.0%
!	TTTTCACTCACAAGTCCTTT+CGG	-	chr8_4:52157310-52157329	Msa1332250:intron	35.0%
	CTCACACAATAAACAAGGAT+GGG	+	chr8_4:52157525-52157544	None:intergenic	35.0%
	TACACACAGCATCAAGTAAA+AGG	+	chr8_4:52157642-52157661	None:intergenic	35.0%
	AAAGTTTCGGTTGGATACAT+GGG	+	chr8_4:52157711-52157730	None:intergenic	35.0%
	GCATAGTTGGATCAAAGTTT+CGG	+	chr8_4:52157724-52157743	None:intergenic	35.0%
	TGATCCAACTATGCTTCTTA+TGG	-	chr8_4:52157730-52157749	Msa1332250:CDS	35.0%
!	TACACCATAAGAAGCATAGT+TGG	+	chr8_4:52157737-52157756	None:intergenic	35.0%
	AACTATGCTTCTTATGGTGT+AGG	-	chr8_4:52157736-52157755	Msa1332250:CDS	35.0%
!!!	CCTACTAGGTTTTTGGTTGT+GGG	+	chr8_4:52155570-52155589	None:intergenic	40.0%
	AAAAACCTAGTAGGCATCAC+CGG	-	chr8_4:52155576-52155595	Msa1332250:three_prime_UTR	40.0%
	TTGAGGAGGATGTTTAAGCA+TGG	-	chr8_4:52155871-52155890	Msa1332250:three_prime_UTR	40.0%
	TGTTCTGTGTCTCATCTAGA+AGG	+	chr8_4:52155897-52155916	None:intergenic	40.0%
	TAAGTCCATTCACCATGCTA+GGG	-	chr8_4:52155999-52156018	Msa1332250:three_prime_UTR	40.0%
	TTATGTGTCCAGTGTAGTGT+CGG	-	chr8_4:52156406-52156425	Msa1332250:intron	40.0%
	AAAATGATACAGGGCCTGTT+TGG	+	chr8_4:52156594-52156613	None:intergenic	40.0%
	ACATTTCCGAACCATGGTAA+AGG	+	chr8_4:52156999-52157018	None:intergenic	40.0%
	TATTGTTTCATAGGGTTGGG+AGG	-	chr8_4:52157230-52157249	Msa1332250:intron	40.0%
	TGAATCAACCCTAACCATGA+AGG	+	chr8_4:52157275-52157294	None:intergenic	40.0%
	TGTGAAGCGAAAGAACCTTA+AGG	-	chr8_4:52157351-52157370	Msa1332250:intron	40.0%
	GATATAAAACGGCTAGAGCA+AGG	+	chr8_4:52157454-52157473	None:intergenic	40.0%
	CCTCACACAATAAACAAGGA+TGG	+	chr8_4:52157526-52157545	None:intergenic	40.0%
!	CCATCCTTGTTTATTGTGTG+AGG	-	chr8_4:52157523-52157542	Msa1332250:CDS	40.0%
	CAAAGTTTCGGTTGGATACA+TGG	+	chr8_4:52157712-52157731	None:intergenic	40.0%
	AGTTGGATCAAAGTTTCGGT+TGG	+	chr8_4:52157720-52157739	None:intergenic	40.0%
!!	TTATCAGTTCTGAAAGGCAC+AGG	+	chr8_4:52157767-52157786	None:intergenic	40.0%
!!!	CCTACTAGGTTTTTGGTTGT+GGG	+	chr8_4:52155570-52155589	None:intergenic	40.0%
	AAAAACCTAGTAGGCATCAC+CGG	-	chr8_4:52155576-52155595	Msa1332250:three_prime_UTR	40.0%
	TTGAGGAGGATGTTTAAGCA+TGG	-	chr8_4:52155871-52155890	Msa1332250:three_prime_UTR	40.0%
	TGTTCTGTGTCTCATCTAGA+AGG	+	chr8_4:52155897-52155916	None:intergenic	40.0%
	TAAGTCCATTCACCATGCTA+GGG	-	chr8_4:52155999-52156018	Msa1332250:three_prime_UTR	40.0%
	TTATGTGTCCAGTGTAGTGT+CGG	-	chr8_4:52156406-52156425	Msa1332250:intron	40.0%
	AAAATGATACAGGGCCTGTT+TGG	+	chr8_4:52156594-52156613	None:intergenic	40.0%
	ACATTTCCGAACCATGGTAA+AGG	+	chr8_4:52156999-52157018	None:intergenic	40.0%
	TATTGTTTCATAGGGTTGGG+AGG	-	chr8_4:52157230-52157249	Msa1332250:intron	40.0%
	TGAATCAACCCTAACCATGA+AGG	+	chr8_4:52157275-52157294	None:intergenic	40.0%
	TGTGAAGCGAAAGAACCTTA+AGG	-	chr8_4:52157351-52157370	Msa1332250:intron	40.0%
	GATATAAAACGGCTAGAGCA+AGG	+	chr8_4:52157454-52157473	None:intergenic	40.0%
	CCTCACACAATAAACAAGGA+TGG	+	chr8_4:52157526-52157545	None:intergenic	40.0%
!	CCATCCTTGTTTATTGTGTG+AGG	-	chr8_4:52157523-52157542	Msa1332250:CDS	40.0%
	CAAAGTTTCGGTTGGATACA+TGG	+	chr8_4:52157712-52157731	None:intergenic	40.0%
	AGTTGGATCAAAGTTTCGGT+TGG	+	chr8_4:52157720-52157739	None:intergenic	40.0%
!!	TTATCAGTTCTGAAAGGCAC+AGG	+	chr8_4:52157767-52157786	None:intergenic	40.0%
	CCCACAACCAAAAACCTAGT+AGG	-	chr8_4:52155567-52155586	Msa1332250:three_prime_UTR	45.0%
!!!	GCCTACTAGGTTTTTGGTTG+TGG	+	chr8_4:52155571-52155590	None:intergenic	45.0%
!	GGTGATGCCTACTAGGTTTT+TGG	+	chr8_4:52155577-52155596	None:intergenic	45.0%
!!	TCCATATGAGAAGGAGCGTT+TGG	-	chr8_4:52155693-52155712	Msa1332250:three_prime_UTR	45.0%
	TCCAAACGCTCCTTCTCATA+TGG	+	chr8_4:52155697-52155716	None:intergenic	45.0%
!	TTTGGATGCTGAGTTGAAGC+TGG	-	chr8_4:52155711-52155730	Msa1332250:three_prime_UTR	45.0%
!	TGCTAGAACGCTTTTGACAC+TGG	-	chr8_4:52155807-52155826	Msa1332250:three_prime_UTR	45.0%
	TGGATGAGAAGAATCCTCGT+CGG	-	chr8_4:52155827-52155846	Msa1332250:three_prime_UTR	45.0%
	CAGACACTCGTCTTCAAATC+TGG	-	chr8_4:52155970-52155989	Msa1332250:three_prime_UTR	45.0%
	ACTCGTCTTCAAATCTGGCA+TGG	-	chr8_4:52155975-52155994	Msa1332250:three_prime_UTR	45.0%
	CTAAGTCCATTCACCATGCT+AGG	-	chr8_4:52155998-52156017	Msa1332250:three_prime_UTR	45.0%
	AAGAACCCTAGCATGGTGAA+TGG	+	chr8_4:52156007-52156026	None:intergenic	45.0%
	ACCATGCTAGGGTTCTTATC+AGG	-	chr8_4:52156010-52156029	Msa1332250:three_prime_UTR	45.0%
	GTACGAAATCTCCTCGGATT+AGG	-	chr8_4:52156313-52156332	Msa1332250:intron	45.0%
!!	TAGTGTCGGTGCTTCATAGA+TGG	-	chr8_4:52156420-52156439	Msa1332250:intron	45.0%
	AATGGAGGTCTCCTTTACCA+TGG	-	chr8_4:52156985-52157004	Msa1332250:intron	45.0%
	AGGTCTCCTTTACCATGGTT+CGG	-	chr8_4:52156990-52157009	Msa1332250:intron	45.0%
	ACATCCCATCCTTCATGGTT+AGG	-	chr8_4:52157263-52157282	Msa1332250:intron	45.0%
	CATCCCATCCTTCATGGTTA+GGG	-	chr8_4:52157264-52157283	Msa1332250:intron	45.0%
	CAACCCTAACCATGAAGGAT+GGG	+	chr8_4:52157270-52157289	None:intergenic	45.0%
	TCAACCCTAACCATGAAGGA+TGG	+	chr8_4:52157271-52157290	None:intergenic	45.0%
	CCCACAACCAAAAACCTAGT+AGG	-	chr8_4:52155567-52155586	Msa1332250:three_prime_UTR	45.0%
!!!	GCCTACTAGGTTTTTGGTTG+TGG	+	chr8_4:52155571-52155590	None:intergenic	45.0%
!	GGTGATGCCTACTAGGTTTT+TGG	+	chr8_4:52155577-52155596	None:intergenic	45.0%
!!	TCCATATGAGAAGGAGCGTT+TGG	-	chr8_4:52155693-52155712	Msa1332250:three_prime_UTR	45.0%
	TCCAAACGCTCCTTCTCATA+TGG	+	chr8_4:52155697-52155716	None:intergenic	45.0%
!	TTTGGATGCTGAGTTGAAGC+TGG	-	chr8_4:52155711-52155730	Msa1332250:three_prime_UTR	45.0%
!	TGCTAGAACGCTTTTGACAC+TGG	-	chr8_4:52155807-52155826	Msa1332250:three_prime_UTR	45.0%
	TGGATGAGAAGAATCCTCGT+CGG	-	chr8_4:52155827-52155846	Msa1332250:three_prime_UTR	45.0%
	CAGACACTCGTCTTCAAATC+TGG	-	chr8_4:52155970-52155989	Msa1332250:three_prime_UTR	45.0%
	ACTCGTCTTCAAATCTGGCA+TGG	-	chr8_4:52155975-52155994	Msa1332250:three_prime_UTR	45.0%
	CTAAGTCCATTCACCATGCT+AGG	-	chr8_4:52155998-52156017	Msa1332250:three_prime_UTR	45.0%
	AAGAACCCTAGCATGGTGAA+TGG	+	chr8_4:52156007-52156026	None:intergenic	45.0%
	ACCATGCTAGGGTTCTTATC+AGG	-	chr8_4:52156010-52156029	Msa1332250:three_prime_UTR	45.0%
	GTACGAAATCTCCTCGGATT+AGG	-	chr8_4:52156313-52156332	Msa1332250:intron	45.0%
!!	TAGTGTCGGTGCTTCATAGA+TGG	-	chr8_4:52156420-52156439	Msa1332250:intron	45.0%
	AATGGAGGTCTCCTTTACCA+TGG	-	chr8_4:52156985-52157004	Msa1332250:intron	45.0%
	AGGTCTCCTTTACCATGGTT+CGG	-	chr8_4:52156990-52157009	Msa1332250:intron	45.0%
	ACATCCCATCCTTCATGGTT+AGG	-	chr8_4:52157263-52157282	Msa1332250:intron	45.0%
	CATCCCATCCTTCATGGTTA+GGG	-	chr8_4:52157264-52157283	Msa1332250:intron	45.0%
	CAACCCTAACCATGAAGGAT+GGG	+	chr8_4:52157270-52157289	None:intergenic	45.0%
	TCAACCCTAACCATGAAGGA+TGG	+	chr8_4:52157271-52157290	None:intergenic	45.0%
	TCCTTCTCATATGGACGACG+AGG	+	chr8_4:52155688-52155707	None:intergenic	50.0%
!	GATGCTGAGTTGAAGCTGGT+CGG	-	chr8_4:52155715-52155734	Msa1332250:three_prime_UTR	50.0%
	TTGAAGCTGGTCGGAGAGTA+TGG	-	chr8_4:52155724-52155743	Msa1332250:three_prime_UTR	50.0%
	AATCCTCGTCGGATCTTCGA+GGG	-	chr8_4:52155838-52155857	Msa1332250:three_prime_UTR	50.0%
!	TCGAGGGTGAAGCTCTTTTG+AGG	-	chr8_4:52155854-52155873	Msa1332250:three_prime_UTR	50.0%
!!	AGGGTGAAGCTCTTTTGAGG+AGG	-	chr8_4:52155857-52155876	Msa1332250:three_prime_UTR	50.0%
	GCCTGATAAGAACCCTAGCA+TGG	+	chr8_4:52156014-52156033	None:intergenic	50.0%
	CTAGGGTTCTTATCAGGCAG+AGG	-	chr8_4:52156016-52156035	Msa1332250:three_prime_UTR	50.0%
!	TTATCAGGCAGAGGCACATC+AGG	-	chr8_4:52156025-52156044	Msa1332250:three_prime_UTR	50.0%
	TTCGCACTGTTGGTCCTGAT+TGG	-	chr8_4:52156225-52156244	Msa1332250:intron	50.0%
!!	TTAGTGTCGATGTCGGACAC+CGG	+	chr8_4:52156347-52156366	None:intergenic	50.0%
!!	ATGAAGCACCGACACTACAC+TGG	+	chr8_4:52156417-52156436	None:intergenic	50.0%
	GATACAGGGCCTGTTTGGAT+TGG	+	chr8_4:52156589-52156608	None:intergenic	50.0%
	GGTGAACATCCCATCCTTCA+TGG	-	chr8_4:52157258-52157277	Msa1332250:intron	50.0%
	TCACTCACAAGTCCTTTCGG+TGG	-	chr8_4:52157313-52157332	Msa1332250:intron	50.0%
	TTCTTTCGCTTCACACGACC+AGG	+	chr8_4:52157346-52157365	None:intergenic	50.0%
	TCCTTCTCATATGGACGACG+AGG	+	chr8_4:52155688-52155707	None:intergenic	50.0%
!	GATGCTGAGTTGAAGCTGGT+CGG	-	chr8_4:52155715-52155734	Msa1332250:three_prime_UTR	50.0%
	TTGAAGCTGGTCGGAGAGTA+TGG	-	chr8_4:52155724-52155743	Msa1332250:three_prime_UTR	50.0%
	AATCCTCGTCGGATCTTCGA+GGG	-	chr8_4:52155838-52155857	Msa1332250:three_prime_UTR	50.0%
!	TCGAGGGTGAAGCTCTTTTG+AGG	-	chr8_4:52155854-52155873	Msa1332250:three_prime_UTR	50.0%
!!	AGGGTGAAGCTCTTTTGAGG+AGG	-	chr8_4:52155857-52155876	Msa1332250:three_prime_UTR	50.0%
	GCCTGATAAGAACCCTAGCA+TGG	+	chr8_4:52156014-52156033	None:intergenic	50.0%
	CTAGGGTTCTTATCAGGCAG+AGG	-	chr8_4:52156016-52156035	Msa1332250:three_prime_UTR	50.0%
!	TTATCAGGCAGAGGCACATC+AGG	-	chr8_4:52156025-52156044	Msa1332250:three_prime_UTR	50.0%
	TTCGCACTGTTGGTCCTGAT+TGG	-	chr8_4:52156225-52156244	Msa1332250:intron	50.0%
!!	TTAGTGTCGATGTCGGACAC+CGG	+	chr8_4:52156347-52156366	None:intergenic	50.0%
!!	ATGAAGCACCGACACTACAC+TGG	+	chr8_4:52156417-52156436	None:intergenic	50.0%
	GATACAGGGCCTGTTTGGAT+TGG	+	chr8_4:52156589-52156608	None:intergenic	50.0%
	GGTGAACATCCCATCCTTCA+TGG	-	chr8_4:52157258-52157277	Msa1332250:intron	50.0%
	TCACTCACAAGTCCTTTCGG+TGG	-	chr8_4:52157313-52157332	Msa1332250:intron	50.0%
	TTCTTTCGCTTCACACGACC+AGG	+	chr8_4:52157346-52157365	None:intergenic	50.0%
	AACCTAGTAGGCATCACCGG+CGG	-	chr8_4:52155579-52155598	Msa1332250:three_prime_UTR	55.0%
	GCCTCGTCGTCCATATGAGA+AGG	-	chr8_4:52155684-52155703	Msa1332250:three_prime_UTR	55.0%
	GAATCCTCGTCGGATCTTCG+AGG	-	chr8_4:52155837-52155856	Msa1332250:three_prime_UTR	55.0%
!	CTCGGATTAGGTGTGTGTCC+CGG	-	chr8_4:52156325-52156344	Msa1332250:intron	55.0%
!!	TAGTGTCGATGTCGGACACC+GGG	+	chr8_4:52156346-52156365	None:intergenic	55.0%
	GTTTCATAGGGTTGGGAGGC+AGG	-	chr8_4:52157234-52157253	Msa1332250:intron	55.0%
	CTCACAAGTCCTTTCGGTGG+TGG	-	chr8_4:52157316-52157335	Msa1332250:intron	55.0%
	CCTTCTTGGCTGCAGCCTTA+AGG	+	chr8_4:52157369-52157388	None:intergenic	55.0%
	CCTTAAGGCTGCAGCCAAGA+AGG	-	chr8_4:52157366-52157385	Msa1332250:intron	55.0%
	ATCACCACCGGAAGCCTTCT+TGG	+	chr8_4:52157383-52157402	None:intergenic	55.0%
	AAGAAGGCTTCCGGTGGTGA+TGG	-	chr8_4:52157382-52157401	Msa1332250:intron	55.0%
!!	TTCCGGTGGTGATGGTGATG+AGG	-	chr8_4:52157390-52157409	Msa1332250:intron	55.0%
	CTCCTCATCACCATCACCAC+CGG	+	chr8_4:52157395-52157414	None:intergenic	55.0%
	AACCTAGTAGGCATCACCGG+CGG	-	chr8_4:52155579-52155598	Msa1332250:three_prime_UTR	55.0%
	GCCTCGTCGTCCATATGAGA+AGG	-	chr8_4:52155684-52155703	Msa1332250:three_prime_UTR	55.0%
	GAATCCTCGTCGGATCTTCG+AGG	-	chr8_4:52155837-52155856	Msa1332250:three_prime_UTR	55.0%
!	CTCGGATTAGGTGTGTGTCC+CGG	-	chr8_4:52156325-52156344	Msa1332250:intron	55.0%
!!	TAGTGTCGATGTCGGACACC+GGG	+	chr8_4:52156346-52156365	None:intergenic	55.0%
	GTTTCATAGGGTTGGGAGGC+AGG	-	chr8_4:52157234-52157253	Msa1332250:intron	55.0%
	CTCACAAGTCCTTTCGGTGG+TGG	-	chr8_4:52157316-52157335	Msa1332250:intron	55.0%
	CCTTCTTGGCTGCAGCCTTA+AGG	+	chr8_4:52157369-52157388	None:intergenic	55.0%
	CCTTAAGGCTGCAGCCAAGA+AGG	-	chr8_4:52157366-52157385	Msa1332250:intron	55.0%
	ATCACCACCGGAAGCCTTCT+TGG	+	chr8_4:52157383-52157402	None:intergenic	55.0%
	AAGAAGGCTTCCGGTGGTGA+TGG	-	chr8_4:52157382-52157401	Msa1332250:intron	55.0%
!!	TTCCGGTGGTGATGGTGATG+AGG	-	chr8_4:52157390-52157409	Msa1332250:intron	55.0%
	CTCCTCATCACCATCACCAC+CGG	+	chr8_4:52157395-52157414	None:intergenic	55.0%
	TTCGCTGCAAGCGCGAGCTT+TGG	-	chr8_4:52155749-52155768	Msa1332250:three_prime_UTR	60.0%
!!	CTGCAAGCGCGAGCTTTGGA+GGG	-	chr8_4:52155753-52155772	Msa1332250:three_prime_UTR	60.0%
	TCACCCTCGAAGATCCGACG+AGG	+	chr8_4:52155844-52155863	None:intergenic	60.0%
	CGGGACACACACCTAATCCG+AGG	+	chr8_4:52156327-52156346	None:intergenic	60.0%
	TCATAGGGTTGGGAGGCAGG+TGG	-	chr8_4:52157237-52157256	Msa1332250:intron	60.0%
	CCAGGAAGACCACCACCGAA+AGG	+	chr8_4:52157328-52157347	None:intergenic	60.0%
!	CCTTTCGGTGGTGGTCTTCC+TGG	-	chr8_4:52157325-52157344	Msa1332250:intron	60.0%
	GCTGCAGCCAAGAAGGCTTC+CGG	-	chr8_4:52157373-52157392	Msa1332250:intron	60.0%
!!	CGGTGGTGATGGTGATGAGG+AGG	-	chr8_4:52157393-52157412	Msa1332250:intron	60.0%
	TTCGCTGCAAGCGCGAGCTT+TGG	-	chr8_4:52155749-52155768	Msa1332250:three_prime_UTR	60.0%
!!	CTGCAAGCGCGAGCTTTGGA+GGG	-	chr8_4:52155753-52155772	Msa1332250:three_prime_UTR	60.0%
	TCACCCTCGAAGATCCGACG+AGG	+	chr8_4:52155844-52155863	None:intergenic	60.0%
	CGGGACACACACCTAATCCG+AGG	+	chr8_4:52156327-52156346	None:intergenic	60.0%
	TCATAGGGTTGGGAGGCAGG+TGG	-	chr8_4:52157237-52157256	Msa1332250:intron	60.0%
	CCAGGAAGACCACCACCGAA+AGG	+	chr8_4:52157328-52157347	None:intergenic	60.0%
!	CCTTTCGGTGGTGGTCTTCC+TGG	-	chr8_4:52157325-52157344	Msa1332250:intron	60.0%
	GCTGCAGCCAAGAAGGCTTC+CGG	-	chr8_4:52157373-52157392	Msa1332250:intron	60.0%
!!	CGGTGGTGATGGTGATGAGG+AGG	-	chr8_4:52157393-52157412	Msa1332250:intron	60.0%
	TGCCGCCGGTGATGCCTACT+AGG	+	chr8_4:52155584-52155603	None:intergenic	65.0%
!	GCTGCAAGCGCGAGCTTTGG+AGG	-	chr8_4:52155752-52155771	Msa1332250:three_prime_UTR	65.0%
	GCAGCCAAGAAGGCTTCCGG+TGG	-	chr8_4:52157376-52157395	Msa1332250:intron	65.0%
	TGCCGCCGGTGATGCCTACT+AGG	+	chr8_4:52155584-52155603	None:intergenic	65.0%
!	GCTGCAAGCGCGAGCTTTGG+AGG	-	chr8_4:52155752-52155771	Msa1332250:three_prime_UTR	65.0%
	GCAGCCAAGAAGGCTTCCGG+TGG	-	chr8_4:52157376-52157395	Msa1332250:intron	65.0%
	GTAGGCATCACCGGCGGCAG+CGG	-	chr8_4:52155585-52155604	Msa1332250:three_prime_UTR	70.0%
	GTAGGCATCACCGGCGGCAG+CGG	-	chr8_4:52155585-52155604	Msa1332250:three_prime_UTR	70.0%
	CATCACCGGCGGCAGCGGCA+AGG	-	chr8_4:52155590-52155609	Msa1332250:three_prime_UTR	75.0%
	CCTCTCCTTGCCGCTGCCGC+CGG	+	chr8_4:52155598-52155617	None:intergenic	75.0%
	CATCACCGGCGGCAGCGGCA+AGG	-	chr8_4:52155590-52155609	Msa1332250:three_prime_UTR	75.0%
	CCTCTCCTTGCCGCTGCCGC+CGG	+	chr8_4:52155598-52155617	None:intergenic	75.0%
!	CCGGCGGCAGCGGCAAGGAG+AGG	-	chr8_4:52155595-52155614	Msa1332250:three_prime_UTR	80.0%
!	CCGGCGGCAGCGGCAAGGAG+AGG	-	chr8_4:52155595-52155614	Msa1332250:three_prime_UTR	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	52155560	52157911	52155560	ID=Msa1332250;Name=Msa1332250
chr8_4	mRNA	52155560	52157911	52155560	ID=Msa1332250-mRNA-1;Parent=Msa1332250;Name=Msa1332250-mRNA-1;_AED=0.01;_eAED=0.01;_QI=77\|1\|1\|1\|1\|1\|2\|485\|197
chr8_4	exon	52155560	52156228	52155560	ID=Msa1332250-mRNA-1:exon:15601;Parent=Msa1332250-mRNA-1
chr8_4	exon	52157425	52157911	52157425	ID=Msa1332250-mRNA-1:exon:15600;Parent=Msa1332250-mRNA-1
chr8_4	five_prime_UTR	52157835	52157911	52157835	ID=Msa1332250-mRNA-1:five_prime_utr;Parent=Msa1332250-mRNA-1
chr8_4	CDS	52157425	52157834	52157425	ID=Msa1332250-mRNA-1:cds;Parent=Msa1332250-mRNA-1
chr8_4	CDS	52156045	52156228	52156045	ID=Msa1332250-mRNA-1:cds;Parent=Msa1332250-mRNA-1
chr8_4	three_prime_UTR	52155560	52156044	52155560	ID=Msa1332250-mRNA-1:three_prime_utr;Parent=Msa1332250-mRNA-1

Gene Sequence

>Msa1332250

ATGGTGCACGTTGCGTTTTATCGAAACTACGGCAAAACATTCAAGAAGCCTCGTCGTCCATATGAGAAGGAGCGTTTGGATGCTGAGTTGAAGCTGGTCGGAGAGTATGGTCTTCGCTGCAAGCGCGAGCTTTGGAGGGTTCAATACGCTCTTAGCCGTATCCGTAACAATGCTAGAACGCTTTTGACACTGGATGAGAAGAATCCTCGTCGGATCTTCGAGGGTGAAGCTCTTTTGAGGAGGATGTTTAAGCATGGCCTTCTAGATGAGACACAGAACAAGCTCGATTATGTTTTGGCTCTTACTGTTGAGAATTTTCTCGAGCGTCGTCTTCAGACACTCGTCTTCAAATCTGGCATGGCTAAGTCCATTCACCATGCTAGGGTTCTTATCAGGCAGAGGCACATCAGGGTTGGGAGGCAGGTGGTGAACATCCCATCCTTCATGGTTAGGGTTGATTCACAGAAGCACATTGACTTTTCACTCACAAGTCCTTTCGGTGGTGGTCTTCCTGGTCGTGTGAAGCGAAAGAACCTTAAGGCTGCAGCCAAGAAGGCTTCCGGTGGTGATGGTGATGAGGAGGATGAAGATTAA

Protein sequence

>Msa1332250

MVHVAFYRNYGKTFKKPRRPYEKERLDAELKLVGEYGLRCKRELWRVQYALSRIRNNARTLLTLDEKNPRRIFEGEALLRRMFKHGLLDETQNKLDYVLALTVENFLERRLQTLVFKSGMAKSIHHARVLIRQRHIRVGRQVVNIPSFMVRVDSQKHIDFSLTSPFGGGLPGRVKRKNLKAAAKKASGGDGDEEDED*