AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0114570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0114570 MtrunA17_Chr1g0195471 91.597 119 9 1 7 125 2 119 2.23e-65 194
Msa0114570 MtrunA17_Chr7g0272321 94.444 108 6 0 18 125 17 124 4.39e-62 186
Msa0114570 MtrunA17_Chr1g0192241 98.305 59 1 0 67 125 18 76 2.61e-26 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0114570 AT3G05560.1 84.800 125 18 1 1 125 1 124 3.87e-63 188
Msa0114570 AT3G05560.3 84.800 125 18 1 1 125 1 124 3.87e-63 188
Msa0114570 AT3G05560.2 84.800 125 18 1 1 125 1 124 3.87e-63 188
Msa0114570 AT5G27770.1 84.694 98 15 0 17 114 16 113 3.97e-49 153
Msa0114570 AT1G02830.1 69.492 118 33 2 1 116 1 117 8.11e-43 137

Find 35 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGATGCTGTTACTGTTTCC+AGG 0.305504 1_3:+31763763 Msa0114570:CDS
TCTTATGTTAGCTTCTCTTT+TGG 0.316391 1_3:+31764930 Msa0114570:three_prime_UTR
GATCTTGTCTTCCACGGGTT+TGG 0.336765 1_3:-31763670 None:intergenic
GAGAAGCTAACATAAGAAAA+TGG 0.346112 1_3:-31764924 None:intergenic
GTTTCTTCAAGAGAGGATTA+AGG 0.367162 1_3:+31763716 Msa0114570:CDS
TTCTGTTCTCTTGTGGCTAA+AGG 0.373476 1_3:+31763474 Msa0114570:five_prime_UTR
ACTTGAAGAAACACAATGTA+AGG 0.413403 1_3:+31764767 Msa0114570:CDS
CACGGGTTTGGCACAGTCAA+TGG 0.421170 1_3:-31763658 None:intergenic
GGTGGTAAACCTGGTGCTCT+TGG 0.432206 1_3:+31763741 Msa0114570:CDS
GTCCATGATCTTGTCTTCCA+CGG 0.443029 1_3:-31763676 None:intergenic
ATGCTCGAGACATGTTGCTT+TGG 0.463943 1_3:+31764960 Msa0114570:three_prime_UTR
AAAATAATCTTAGACATTCA+AGG 0.465501 1_3:-31765110 None:intergenic
TGTTCTGTTCTGTTCTCTTG+TGG 0.495042 1_3:+31763467 Msa0114570:five_prime_UTR
ATTAAGGTTGGTGGTAAACC+TGG 0.503896 1_3:+31763732 Msa0114570:CDS
GTAGATGCAGCTGGTTCAAA+GGG 0.506848 1_3:+31763609 Msa0114570:CDS
TGGTTCAAAGGGAAAGAAGA+AGG 0.510234 1_3:+31763620 Msa0114570:CDS
CTGATAGCAACTTCTCCAAA+CGG 0.520527 1_3:+31763811 Msa0114570:CDS
AGAAACACAATGTAAGGGAT+TGG 0.522187 1_3:+31764773 Msa0114570:CDS
TCCATGATCTTGTCTTCCAC+GGG 0.522466 1_3:-31763675 None:intergenic
TCGAAAAGTTTCTTCAAGAG+AGG 0.523656 1_3:+31763709 Msa0114570:CDS
CAAGAGAGGATTAAGGTTGG+TGG 0.536618 1_3:+31763723 Msa0114570:CDS
AGTAGATGCAGCTGGTTCAA+AGG 0.536758 1_3:+31763608 Msa0114570:CDS
TTCAACATTGCTGAGAATGA+AGG 0.551218 1_3:+31764847 Msa0114570:CDS
ACCCGTGGAAGACAAGATCA+TGG 0.560910 1_3:+31763674 Msa0114570:CDS
CTTCAAGAGAGGATTAAGGT+TGG 0.564429 1_3:+31763720 Msa0114570:CDS
ATGGGTCGAGTAGATGCAGC+TGG 0.584016 1_3:+31763600 Msa0114570:CDS
CAGAACACAGCACAATGTGA+AGG 0.593257 1_3:-31763446 None:intergenic
ACAGCATCACCAAGAGCACC+AGG 0.602480 1_3:-31763750 None:intergenic
ATAGAAATGTTTATGAGCTG+AGG 0.605170 1_3:+31764821 Msa0114570:CDS
TGATGCTGTTACTGTTTCCA+GGG 0.614918 1_3:+31763764 Msa0114570:CDS
AGAACACAGCACAATGTGAA+GGG 0.618440 1_3:-31763445 None:intergenic
GGTTCAAAGGGAAAGAAGAA+GGG 0.618619 1_3:+31763621 Msa0114570:CDS
CATTGACTGTGCCAAACCCG+TGG 0.627134 1_3:+31763659 Msa0114570:CDS
CTTGAAGAAACACAATGTAA+GGG 0.629064 1_3:+31764768 Msa0114570:CDS
CATTGCTGAGAATGAAGGAG+AGG 0.630620 1_3:+31764852 Msa0114570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGATTAATTGAAATGAAA+GGG - chr1_3:31763518-31763537 None:intergenic 15.0%
!! TTATGATTAATTGAAATGAA+AGG - chr1_3:31763519-31763538 None:intergenic 15.0%
!!! AATGTTAAAATTGATTCTAA+AGG - chr1_3:31764146-31764165 None:intergenic 15.0%
!!! GGATAATTTTTTTGAAAATA+AGG - chr1_3:31764545-31764564 None:intergenic 15.0%
!! TATGATTAATTGAAATGAAA+GGG - chr1_3:31763518-31763537 None:intergenic 15.0%
!! TTATGATTAATTGAAATGAA+AGG - chr1_3:31763519-31763538 None:intergenic 15.0%
!!! AATGTTAAAATTGATTCTAA+AGG - chr1_3:31764146-31764165 None:intergenic 15.0%
!!! GGATAATTTTTTTGAAAATA+AGG - chr1_3:31764545-31764564 None:intergenic 15.0%
!! ATGATTAATTGAAATGAAAG+GGG - chr1_3:31763517-31763536 None:intergenic 20.0%
!!! AGTTTTTTGTTTTGTTTGAT+TGG + chr1_3:31763880-31763899 Msa0114570:intron 20.0%
!!! ATTTAGGTTGTTTTTTATGA+TGG + chr1_3:31763941-31763960 Msa0114570:intron 20.0%
!!! AATTTTAACATTACTAGAAG+TGG + chr1_3:31764154-31764173 Msa0114570:intron 20.0%
!!! TGATTTTGTAAAACTAATGT+TGG - chr1_3:31764212-31764231 None:intergenic 20.0%
!!! TTATGTTAACTTTTGATTCA+TGG + chr1_3:31764390-31764409 Msa0114570:intron 20.0%
!!! CCAATAATATTAGAAGTTTT+TGG - chr1_3:31764456-31764475 None:intergenic 20.0%
!! CCAAAAACTTCTAATATTAT+TGG + chr1_3:31764453-31764472 Msa0114570:intron 20.0%
!!! TTTTGTTCAATTAAGAATTG+CGG + chr1_3:31765006-31765025 Msa0114570:three_prime_UTR 20.0%
!! ATGATTAATTGAAATGAAAG+GGG - chr1_3:31763517-31763536 None:intergenic 20.0%
!!! AGTTTTTTGTTTTGTTTGAT+TGG + chr1_3:31763880-31763899 Msa0114570:intron 20.0%
!!! ATTTAGGTTGTTTTTTATGA+TGG + chr1_3:31763941-31763960 Msa0114570:intron 20.0%
!!! AATTTTAACATTACTAGAAG+TGG + chr1_3:31764154-31764173 Msa0114570:intron 20.0%
!!! TGATTTTGTAAAACTAATGT+TGG - chr1_3:31764212-31764231 None:intergenic 20.0%
!!! TTATGTTAACTTTTGATTCA+TGG + chr1_3:31764390-31764409 Msa0114570:intron 20.0%
!!! CCAATAATATTAGAAGTTTT+TGG - chr1_3:31764456-31764475 None:intergenic 20.0%
!! CCAAAAACTTCTAATATTAT+TGG + chr1_3:31764453-31764472 Msa0114570:intron 20.0%
!!! TTTTGTTCAATTAAGAATTG+CGG + chr1_3:31765006-31765025 Msa0114570:three_prime_UTR 20.0%
! TCATTCATAGAAGACAAAAA+TGG + chr1_3:31763581-31763600 Msa0114570:intron 25.0%
! CATTCATAGAAGACAAAAAT+GGG + chr1_3:31763582-31763601 Msa0114570:intron 25.0%
!!! TTTTTGTTTTGTTTGATTGG+AGG + chr1_3:31763883-31763902 Msa0114570:intron 25.0%
!! GGAATTCAATTGTTTGATTT+AGG + chr1_3:31763925-31763944 Msa0114570:intron 25.0%
!! TAAAATTGATTCTAAAGGTG+TGG - chr1_3:31764141-31764160 None:intergenic 25.0%
!!! ATTGATTTTACAATCTCGTT+TGG + chr1_3:31764275-31764294 Msa0114570:intron 25.0%
!! TTGATTTTACAATCTCGTTT+GGG + chr1_3:31764276-31764295 Msa0114570:intron 25.0%
!! ATATTATTGGCAATTTTGTG+TGG + chr1_3:31764466-31764485 Msa0114570:intron 25.0%
!! TATTATTGGCAATTTTGTGT+GGG + chr1_3:31764467-31764486 Msa0114570:intron 25.0%
! TCATTCATAGAAGACAAAAA+TGG + chr1_3:31763581-31763600 Msa0114570:intron 25.0%
! CATTCATAGAAGACAAAAAT+GGG + chr1_3:31763582-31763601 Msa0114570:intron 25.0%
!!! TTTTTGTTTTGTTTGATTGG+AGG + chr1_3:31763883-31763902 Msa0114570:intron 25.0%
!! GGAATTCAATTGTTTGATTT+AGG + chr1_3:31763925-31763944 Msa0114570:intron 25.0%
!! TAAAATTGATTCTAAAGGTG+TGG - chr1_3:31764141-31764160 None:intergenic 25.0%
!!! ATTGATTTTACAATCTCGTT+TGG + chr1_3:31764275-31764294 Msa0114570:intron 25.0%
!! TTGATTTTACAATCTCGTTT+GGG + chr1_3:31764276-31764295 Msa0114570:intron 25.0%
!! ATATTATTGGCAATTTTGTG+TGG + chr1_3:31764466-31764485 Msa0114570:intron 25.0%
!! TATTATTGGCAATTTTGTGT+GGG + chr1_3:31764467-31764486 Msa0114570:intron 25.0%
AATTGAAATGAAAGGGGAAA+AGG - chr1_3:31763511-31763530 None:intergenic 30.0%
TCGAGATTCAATTTCAGTTT+CGG - chr1_3:31763554-31763573 None:intergenic 30.0%
AAGAATAGAGAATAACCGTT+TGG - chr1_3:31763829-31763848 None:intergenic 30.0%
! GGATTTGTGTTTTCTTAGTT+AGG + chr1_3:31763904-31763923 Msa0114570:intron 30.0%
!!! TGTTTTTTATGATGGTTCTG+CGG + chr1_3:31763949-31763968 Msa0114570:intron 30.0%
!!! AATGTGTGTGTTTGGTTTTA+GGG - chr1_3:31764258-31764277 None:intergenic 30.0%
GTAAAATCAATGTGTGTGTT+TGG - chr1_3:31764266-31764285 None:intergenic 30.0%
TTCATGGAATATACAATCTC+TGG + chr1_3:31764406-31764425 Msa0114570:intron 30.0%
TCATGGAATATACAATCTCT+GGG + chr1_3:31764407-31764426 Msa0114570:intron 30.0%
! ATTATTGGCAATTTTGTGTG+GGG + chr1_3:31764468-31764487 Msa0114570:intron 30.0%
TTCTGTCTAAATTGCAATGA+TGG + chr1_3:31764496-31764515 Msa0114570:intron 30.0%
TCTGTCTAAATTGCAATGAT+GGG + chr1_3:31764497-31764516 Msa0114570:intron 30.0%
TGCAATTGTTCGTATTTAGA+TGG + chr1_3:31764590-31764609 Msa0114570:intron 30.0%
GCAATTGTTCGTATTTAGAT+GGG + chr1_3:31764591-31764610 Msa0114570:intron 30.0%
CAATTGTTCGTATTTAGATG+GGG + chr1_3:31764592-31764611 Msa0114570:intron 30.0%
!!! ACACTGTTTTTGTGATCTAA+AGG + chr1_3:31764680-31764699 Msa0114570:intron 30.0%
ACGAGTTGTTAAAAAAAGCA+TGG - chr1_3:31764711-31764730 None:intergenic 30.0%
! GTGTTTCTTCAAGTACTTTT+TGG - chr1_3:31764761-31764780 None:intergenic 30.0%
ACTTGAAGAAACACAATGTA+AGG + chr1_3:31764767-31764786 Msa0114570:CDS 30.0%
CTTGAAGAAACACAATGTAA+GGG + chr1_3:31764768-31764787 Msa0114570:CDS 30.0%
ATAGAAATGTTTATGAGCTG+AGG + chr1_3:31764821-31764840 Msa0114570:CDS 30.0%
GAGAAGCTAACATAAGAAAA+TGG - chr1_3:31764927-31764946 None:intergenic 30.0%
!! TCTTATGTTAGCTTCTCTTT+TGG + chr1_3:31764930-31764949 Msa0114570:three_prime_UTR 30.0%
! GTTACCAAAAAGAGTATAGA+AGG - chr1_3:31765085-31765104 None:intergenic 30.0%
AATTGAAATGAAAGGGGAAA+AGG - chr1_3:31763511-31763530 None:intergenic 30.0%
TCGAGATTCAATTTCAGTTT+CGG - chr1_3:31763554-31763573 None:intergenic 30.0%
AAGAATAGAGAATAACCGTT+TGG - chr1_3:31763829-31763848 None:intergenic 30.0%
! GGATTTGTGTTTTCTTAGTT+AGG + chr1_3:31763904-31763923 Msa0114570:intron 30.0%
!!! TGTTTTTTATGATGGTTCTG+CGG + chr1_3:31763949-31763968 Msa0114570:intron 30.0%
!!! AATGTGTGTGTTTGGTTTTA+GGG - chr1_3:31764258-31764277 None:intergenic 30.0%
GTAAAATCAATGTGTGTGTT+TGG - chr1_3:31764266-31764285 None:intergenic 30.0%
TTCATGGAATATACAATCTC+TGG + chr1_3:31764406-31764425 Msa0114570:intron 30.0%
TCATGGAATATACAATCTCT+GGG + chr1_3:31764407-31764426 Msa0114570:intron 30.0%
! ATTATTGGCAATTTTGTGTG+GGG + chr1_3:31764468-31764487 Msa0114570:intron 30.0%
TTCTGTCTAAATTGCAATGA+TGG + chr1_3:31764496-31764515 Msa0114570:intron 30.0%
TCTGTCTAAATTGCAATGAT+GGG + chr1_3:31764497-31764516 Msa0114570:intron 30.0%
TGCAATTGTTCGTATTTAGA+TGG + chr1_3:31764590-31764609 Msa0114570:intron 30.0%
GCAATTGTTCGTATTTAGAT+GGG + chr1_3:31764591-31764610 Msa0114570:intron 30.0%
CAATTGTTCGTATTTAGATG+GGG + chr1_3:31764592-31764611 Msa0114570:intron 30.0%
!!! ACACTGTTTTTGTGATCTAA+AGG + chr1_3:31764680-31764699 Msa0114570:intron 30.0%
ACGAGTTGTTAAAAAAAGCA+TGG - chr1_3:31764711-31764730 None:intergenic 30.0%
! GTGTTTCTTCAAGTACTTTT+TGG - chr1_3:31764761-31764780 None:intergenic 30.0%
ACTTGAAGAAACACAATGTA+AGG + chr1_3:31764767-31764786 Msa0114570:CDS 30.0%
CTTGAAGAAACACAATGTAA+GGG + chr1_3:31764768-31764787 Msa0114570:CDS 30.0%
ATAGAAATGTTTATGAGCTG+AGG + chr1_3:31764821-31764840 Msa0114570:CDS 30.0%
GAGAAGCTAACATAAGAAAA+TGG - chr1_3:31764927-31764946 None:intergenic 30.0%
!! TCTTATGTTAGCTTCTCTTT+TGG + chr1_3:31764930-31764949 Msa0114570:three_prime_UTR 30.0%
! GTTACCAAAAAGAGTATAGA+AGG - chr1_3:31765085-31765104 None:intergenic 30.0%
ATGTGAAGGGAAAAAATGGA+CGG - chr1_3:31763435-31763454 None:intergenic 35.0%
CACAATGTGAAGGGAAAAAA+TGG - chr1_3:31763439-31763458 None:intergenic 35.0%
AGATTCAATTTCAGTTTCGG+TGG - chr1_3:31763551-31763570 None:intergenic 35.0%
TCGAAAAGTTTCTTCAAGAG+AGG + chr1_3:31763709-31763728 Msa0114570:CDS 35.0%
GTTTCTTCAAGAGAGGATTA+AGG + chr1_3:31763716-31763735 Msa0114570:CDS 35.0%
!!! AGTAATCTTGCTTTTTTCCC+TGG - chr1_3:31763784-31763803 None:intergenic 35.0%
TCCGATTAAAAGTGAGTTGT+AGG + chr1_3:31764000-31764019 Msa0114570:intron 35.0%
!! CCCTACAACTCACTTTTAAT+CGG - chr1_3:31764004-31764023 None:intergenic 35.0%
CCGATTAAAAGTGAGTTGTA+GGG + chr1_3:31764001-31764020 Msa0114570:intron 35.0%
TAGGGAAAGTGAATTGTGTT+TGG + chr1_3:31764019-31764038 Msa0114570:intron 35.0%
TGAATTGAAGATGGTAGGTT+TGG - chr1_3:31764182-31764201 None:intergenic 35.0%
AAGAGTGAATTGAAGATGGT+AGG - chr1_3:31764187-31764206 None:intergenic 35.0%
!!! CAATGTGTGTGTTTGGTTTT+AGG - chr1_3:31764259-31764278 None:intergenic 35.0%
! ATTTTACAATCTCGTTTGGG+TGG + chr1_3:31764279-31764298 Msa0114570:intron 35.0%
CATATTGCGATGATGCTAAA+AGG - chr1_3:31764331-31764350 None:intergenic 35.0%
AAATTATCCCCTGCTTCAAA+AGG + chr1_3:31764556-31764575 Msa0114570:intron 35.0%
!!! AACAAAAGCCTTTTGAAGCA+GGG - chr1_3:31764567-31764586 None:intergenic 35.0%
TTAACAACTCGTATTGTTCC+AGG + chr1_3:31764719-31764738 Msa0114570:intron 35.0%
AGAAACACAATGTAAGGGAT+TGG + chr1_3:31764773-31764792 Msa0114570:CDS 35.0%
TTCAACATTGCTGAGAATGA+AGG + chr1_3:31764847-31764866 Msa0114570:CDS 35.0%
! AATTGCGGTAACACTTTTGT+TGG + chr1_3:31765021-31765040 Msa0114570:three_prime_UTR 35.0%
ATGTGAAGGGAAAAAATGGA+CGG - chr1_3:31763435-31763454 None:intergenic 35.0%
CACAATGTGAAGGGAAAAAA+TGG - chr1_3:31763439-31763458 None:intergenic 35.0%
AGATTCAATTTCAGTTTCGG+TGG - chr1_3:31763551-31763570 None:intergenic 35.0%
TCGAAAAGTTTCTTCAAGAG+AGG + chr1_3:31763709-31763728 Msa0114570:CDS 35.0%
GTTTCTTCAAGAGAGGATTA+AGG + chr1_3:31763716-31763735 Msa0114570:CDS 35.0%
!!! AGTAATCTTGCTTTTTTCCC+TGG - chr1_3:31763784-31763803 None:intergenic 35.0%
TCCGATTAAAAGTGAGTTGT+AGG + chr1_3:31764000-31764019 Msa0114570:intron 35.0%
!! CCCTACAACTCACTTTTAAT+CGG - chr1_3:31764004-31764023 None:intergenic 35.0%
CCGATTAAAAGTGAGTTGTA+GGG + chr1_3:31764001-31764020 Msa0114570:intron 35.0%
TAGGGAAAGTGAATTGTGTT+TGG + chr1_3:31764019-31764038 Msa0114570:intron 35.0%
TGAATTGAAGATGGTAGGTT+TGG - chr1_3:31764182-31764201 None:intergenic 35.0%
AAGAGTGAATTGAAGATGGT+AGG - chr1_3:31764187-31764206 None:intergenic 35.0%
!!! CAATGTGTGTGTTTGGTTTT+AGG - chr1_3:31764259-31764278 None:intergenic 35.0%
! ATTTTACAATCTCGTTTGGG+TGG + chr1_3:31764279-31764298 Msa0114570:intron 35.0%
CATATTGCGATGATGCTAAA+AGG - chr1_3:31764331-31764350 None:intergenic 35.0%
AAATTATCCCCTGCTTCAAA+AGG + chr1_3:31764556-31764575 Msa0114570:intron 35.0%
!!! AACAAAAGCCTTTTGAAGCA+GGG - chr1_3:31764567-31764586 None:intergenic 35.0%
TTAACAACTCGTATTGTTCC+AGG + chr1_3:31764719-31764738 Msa0114570:intron 35.0%
AGAAACACAATGTAAGGGAT+TGG + chr1_3:31764773-31764792 Msa0114570:CDS 35.0%
TTCAACATTGCTGAGAATGA+AGG + chr1_3:31764847-31764866 Msa0114570:CDS 35.0%
! AATTGCGGTAACACTTTTGT+TGG + chr1_3:31765021-31765040 Msa0114570:three_prime_UTR 35.0%
AGAACACAGCACAATGTGAA+GGG - chr1_3:31763448-31763467 None:intergenic 40.0%
TGTTCTGTTCTGTTCTCTTG+TGG + chr1_3:31763467-31763486 Msa0114570:five_prime_UTR 40.0%
TTCTGTTCTCTTGTGGCTAA+AGG + chr1_3:31763474-31763493 Msa0114570:five_prime_UTR 40.0%
TGGTTCAAAGGGAAAGAAGA+AGG + chr1_3:31763620-31763639 Msa0114570:CDS 40.0%
GGTTCAAAGGGAAAGAAGAA+GGG + chr1_3:31763621-31763640 Msa0114570:CDS 40.0%
CTTCAAGAGAGGATTAAGGT+TGG + chr1_3:31763720-31763739 Msa0114570:CDS 40.0%
!! ATTAAGGTTGGTGGTAAACC+TGG + chr1_3:31763732-31763751 Msa0114570:CDS 40.0%
TGATGCTGTTACTGTTTCCA+GGG + chr1_3:31763764-31763783 Msa0114570:CDS 40.0%
CTGATAGCAACTTCTCCAAA+CGG + chr1_3:31763811-31763830 Msa0114570:CDS 40.0%
GGCTAAGAGTGAATTGAAGA+TGG - chr1_3:31764191-31764210 None:intergenic 40.0%
GTTTGGGTGGTATTCAAAAC+TGG + chr1_3:31764292-31764311 Msa0114570:intron 40.0%
!!! ACAAAAGCCTTTTGAAGCAG+GGG - chr1_3:31764566-31764585 None:intergenic 40.0%
!!! CAACAAAAGCCTTTTGAAGC+AGG - chr1_3:31764568-31764587 None:intergenic 40.0%
ATCAGTCTGTCTTCAACATC+TGG + chr1_3:31764643-31764662 Msa0114570:intron 40.0%
GGTCAAATACTTGAGATACC+TGG - chr1_3:31764740-31764759 None:intergenic 40.0%
!! GTTAGCTTCTCTTTTGGCTA+TGG + chr1_3:31764936-31764955 Msa0114570:three_prime_UTR 40.0%
! CGGTAACACTTTTGTTGGAA+TGG + chr1_3:31765026-31765045 Msa0114570:three_prime_UTR 40.0%
! AGCGCCTTCTATACTCTTTT+TGG + chr1_3:31765078-31765097 Msa0114570:three_prime_UTR 40.0%
AGAACACAGCACAATGTGAA+GGG - chr1_3:31763448-31763467 None:intergenic 40.0%
TGTTCTGTTCTGTTCTCTTG+TGG + chr1_3:31763467-31763486 Msa0114570:five_prime_UTR 40.0%
TTCTGTTCTCTTGTGGCTAA+AGG + chr1_3:31763474-31763493 Msa0114570:five_prime_UTR 40.0%
TGGTTCAAAGGGAAAGAAGA+AGG + chr1_3:31763620-31763639 Msa0114570:CDS 40.0%
GGTTCAAAGGGAAAGAAGAA+GGG + chr1_3:31763621-31763640 Msa0114570:CDS 40.0%
CTTCAAGAGAGGATTAAGGT+TGG + chr1_3:31763720-31763739 Msa0114570:CDS 40.0%
!! ATTAAGGTTGGTGGTAAACC+TGG + chr1_3:31763732-31763751 Msa0114570:CDS 40.0%
TGATGCTGTTACTGTTTCCA+GGG + chr1_3:31763764-31763783 Msa0114570:CDS 40.0%
CTGATAGCAACTTCTCCAAA+CGG + chr1_3:31763811-31763830 Msa0114570:CDS 40.0%
GGCTAAGAGTGAATTGAAGA+TGG - chr1_3:31764191-31764210 None:intergenic 40.0%
GTTTGGGTGGTATTCAAAAC+TGG + chr1_3:31764292-31764311 Msa0114570:intron 40.0%
!!! ACAAAAGCCTTTTGAAGCAG+GGG - chr1_3:31764566-31764585 None:intergenic 40.0%
!!! CAACAAAAGCCTTTTGAAGC+AGG - chr1_3:31764568-31764587 None:intergenic 40.0%
ATCAGTCTGTCTTCAACATC+TGG + chr1_3:31764643-31764662 Msa0114570:intron 40.0%
GGTCAAATACTTGAGATACC+TGG - chr1_3:31764740-31764759 None:intergenic 40.0%
!! GTTAGCTTCTCTTTTGGCTA+TGG + chr1_3:31764936-31764955 Msa0114570:three_prime_UTR 40.0%
! CGGTAACACTTTTGTTGGAA+TGG + chr1_3:31765026-31765045 Msa0114570:three_prime_UTR 40.0%
! AGCGCCTTCTATACTCTTTT+TGG + chr1_3:31765078-31765097 Msa0114570:three_prime_UTR 40.0%
CAGAACACAGCACAATGTGA+AGG - chr1_3:31763449-31763468 None:intergenic 45.0%
! AGTAGATGCAGCTGGTTCAA+AGG + chr1_3:31763608-31763627 Msa0114570:CDS 45.0%
! GTAGATGCAGCTGGTTCAAA+GGG + chr1_3:31763609-31763628 Msa0114570:CDS 45.0%
TCCATGATCTTGTCTTCCAC+GGG - chr1_3:31763678-31763697 None:intergenic 45.0%
GTCCATGATCTTGTCTTCCA+CGG - chr1_3:31763679-31763698 None:intergenic 45.0%
CAAGAGAGGATTAAGGTTGG+TGG + chr1_3:31763723-31763742 Msa0114570:CDS 45.0%
GTGATGCTGTTACTGTTTCC+AGG + chr1_3:31763763-31763782 Msa0114570:CDS 45.0%
CATTGCTGAGAATGAAGGAG+AGG + chr1_3:31764852-31764871 Msa0114570:CDS 45.0%
! ATGCTCGAGACATGTTGCTT+TGG + chr1_3:31764960-31764979 Msa0114570:three_prime_UTR 45.0%
CAGAACACAGCACAATGTGA+AGG - chr1_3:31763449-31763468 None:intergenic 45.0%
! AGTAGATGCAGCTGGTTCAA+AGG + chr1_3:31763608-31763627 Msa0114570:CDS 45.0%
! GTAGATGCAGCTGGTTCAAA+GGG + chr1_3:31763609-31763628 Msa0114570:CDS 45.0%
TCCATGATCTTGTCTTCCAC+GGG - chr1_3:31763678-31763697 None:intergenic 45.0%
GTCCATGATCTTGTCTTCCA+CGG - chr1_3:31763679-31763698 None:intergenic 45.0%
CAAGAGAGGATTAAGGTTGG+TGG + chr1_3:31763723-31763742 Msa0114570:CDS 45.0%
GTGATGCTGTTACTGTTTCC+AGG + chr1_3:31763763-31763782 Msa0114570:CDS 45.0%
CATTGCTGAGAATGAAGGAG+AGG + chr1_3:31764852-31764871 Msa0114570:CDS 45.0%
! ATGCTCGAGACATGTTGCTT+TGG + chr1_3:31764960-31764979 Msa0114570:three_prime_UTR 45.0%
GATCTTGTCTTCCACGGGTT+TGG - chr1_3:31763673-31763692 None:intergenic 50.0%
ACCCGTGGAAGACAAGATCA+TGG + chr1_3:31763674-31763693 Msa0114570:CDS 50.0%
GATCTTGTCTTCCACGGGTT+TGG - chr1_3:31763673-31763692 None:intergenic 50.0%
ACCCGTGGAAGACAAGATCA+TGG + chr1_3:31763674-31763693 Msa0114570:CDS 50.0%
ATGGGTCGAGTAGATGCAGC+TGG + chr1_3:31763600-31763619 Msa0114570:CDS 55.0%
CACGGGTTTGGCACAGTCAA+TGG - chr1_3:31763661-31763680 None:intergenic 55.0%
! CATTGACTGTGCCAAACCCG+TGG + chr1_3:31763659-31763678 Msa0114570:CDS 55.0%
GGTGGTAAACCTGGTGCTCT+TGG + chr1_3:31763741-31763760 Msa0114570:CDS 55.0%
!! ACAGCATCACCAAGAGCACC+AGG - chr1_3:31763753-31763772 None:intergenic 55.0%
ATGGGTCGAGTAGATGCAGC+TGG + chr1_3:31763600-31763619 Msa0114570:CDS 55.0%
CACGGGTTTGGCACAGTCAA+TGG - chr1_3:31763661-31763680 None:intergenic 55.0%
! CATTGACTGTGCCAAACCCG+TGG + chr1_3:31763659-31763678 Msa0114570:CDS 55.0%
GGTGGTAAACCTGGTGCTCT+TGG + chr1_3:31763741-31763760 Msa0114570:CDS 55.0%
!! ACAGCATCACCAAGAGCACC+AGG - chr1_3:31763753-31763772 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 31763429 31765131 31763429 ID=Msa0114570;Name=Msa0114570
chr1_3 mRNA 31763429 31765131 31763429 ID=Msa0114570-mRNA-1;Parent=Msa0114570;Name=Msa0114570-mRNA-1;_AED=0.00;_eAED=0.00;_QI=76|1|1|1|0.5|0.33|3|246|125
chr1_3 exon 31763429 31763495 31763429 ID=Msa0114570-mRNA-1:exon:10313;Parent=Msa0114570-mRNA-1
chr1_3 exon 31763591 31763832 31763591 ID=Msa0114570-mRNA-1:exon:10314;Parent=Msa0114570-mRNA-1
chr1_3 exon 31764741 31765131 31764741 ID=Msa0114570-mRNA-1:exon:10315;Parent=Msa0114570-mRNA-1
chr1_3 five_prime_UTR 31763429 31763495 31763429 ID=Msa0114570-mRNA-1:five_prime_utr;Parent=Msa0114570-mRNA-1
chr1_3 five_prime_UTR 31763591 31763599 31763591 ID=Msa0114570-mRNA-1:five_prime_utr;Parent=Msa0114570-mRNA-1
chr1_3 CDS 31763600 31763832 31763600 ID=Msa0114570-mRNA-1:cds;Parent=Msa0114570-mRNA-1
chr1_3 CDS 31764741 31764885 31764741 ID=Msa0114570-mRNA-1:cds;Parent=Msa0114570-mRNA-1
chr1_3 three_prime_UTR 31764886 31765131 31764886 ID=Msa0114570-mRNA-1:three_prime_utr;Parent=Msa0114570-mRNA-1
Gene Sequence

>Msa0114570

ATGGGTCGAGTAGATGCAGCTGGTTCAAAGGGAAAGAAGAAGGGAGTGATAACATTCACCATTGACTGTGCCAAACCCGTGGAAGACAAGATCATGGACATCGCTTCTCTCGAAAAGTTTCTTCAAGAGAGGATTAAGGTTGGTGGTAAACCTGGTGCTCTTGGTGATGCTGTTACTGTTTCCAGGGAAAAAAGCAAGATTACTGTTACTTCTGATAGCAACTTCTCCAAACGGTATCTCAAGTATTTGACCAAAAAGTACTTGAAGAAACACAATGTAAGGGATTGGCTCAGAGTGATTGCTTCAAACAAAGATAGAAATGTTTATGAGCTGAGGTACTTCAACATTGCTGAGAATGAAGGAGAGGAAGAAGACTGA
Protein sequence

>Msa0114570

MGRVDAAGSKGKKKGVITFTIDCAKPVEDKIMDIASLEKFLQERIKVGGKPGALGDAVTVSREKSKITVTSDSNFSKRYLKYLTKKYLKKHNVRDWLRVIASNKDRNVYELRYFNIAENEGEEED*