AlfalfaGEDB | Msa1415460

M. sativa cultivar zhongmu-4 / Msa1415460

KEGG: K12479
GO: GO:0000139 GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0009705 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0031090 GO:0031984 GO:0032588 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0098588 GO:0098791 GO:0098805
PFAM: PF00995.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1415460	sp\|O49048\|VPS45_ARATH	84.359	569	88	1	1	568	1	569	0.0	997

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1415460	A0A072VB81	99.648	568	2	0	1	568	1	568	0.0	1165

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0141180	Msa1415460	0.975728	1.732961e-140	-8.615850e-47
Msa0205010	Msa1415460	0.811612	6.418182e-51	-8.615850e-47
Msa0375710	Msa1415460	0.834121	3.600008e-56	-8.615850e-47
Msa0424410	Msa1415460	0.953746	1.387002e-111	-8.615850e-47
Msa0569960	Msa1415460	0.800200	1.611491e-48	-8.615850e-47
Msa0690310	Msa1415460	0.804895	1.734213e-49	-8.615850e-47
Msa0741440	Msa1415460	0.803158	3.983465e-49	-8.615850e-47
Msa0786750	Msa1415460	0.825138	5.518762e-54	-8.615850e-47
Msa0822500	Msa1415460	0.818525	1.873273e-52	-8.615850e-47
Msa1207310	Msa1415460	0.811148	8.091703e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1415460	MtrunA17_Chr3g0126071	99.472	568	2	1	1	568	1	567	0.0	1158
Msa1415460	MtrunA17_Chr4g0056851	23.404	517	346	17	19	499	146	648	4.65e-25	110
Msa1415460	MtrunA17_Chr4g0051171	23.614	559	351	21	22	525	39	576	8.96e-24	105
Msa1415460	MtrunA17_Chr5g0398701	23.776	429	281	13	19	414	43	458	1.69e-22	102

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1415460	AT1G77140.1	84.359	569	88	1	1	568	1	569	0.0	997
Msa1415460	AT1G02010.3	24.405	504	343	16	17	494	41	532	1.49e-32	132
Msa1415460	AT1G02010.2	24.206	504	344	16	17	494	41	532	1.94e-32	132
Msa1415460	AT1G02010.5	24.405	504	343	16	17	494	9	500	2.12e-32	132
Msa1415460	AT1G02010.1	24.206	504	344	16	17	494	41	532	2.74e-32	132
Msa1415460	AT1G02010.4	23.738	535	338	19	17	494	41	562	1.65e-31	130
Msa1415460	AT4G12120.1	23.397	624	370	20	22	556	44	648	2.68e-30	126
Msa1415460	AT1G12360.1	24.277	519	338	16	19	499	41	542	8.57e-30	124
Msa1415460	AT2G17980.1	24.833	600	355	23	22	554	35	605	5.63e-25	109
Msa1415460	AT1G02010.6	24.784	347	230	12	170	494	46	383	2.10e-22	101

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Find 153 sgRNAs with CRISPR-Local

Find 459 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGAGGGACGAGCCAT+GGG	0.565367	tig0024227:+26562	None:intergenic
AAAAGCTGCATTAATTGAAC+AGG	0.355320	tig0024227:+28773	None:intergenic
AAAAGTACAAACCTACTGTT+TGG	0.401418	tig0024227:+30022	None:intergenic
AAACCGAACGCCTGTATTAC+TGG	0.282191	tig0024227:+27560	None:intergenic
AAAGCTGCATTAATTGAACA+GGG	0.678420	tig0024227:+28774	None:intergenic
AAAGTGGACGTCAAATCCAT+TGG	0.576391	tig0024227:-30298	Msa1415460:CDS
AAATAACACCCAAATTTATT+CGG	0.360837	tig0024227:+26188	None:intergenic
AAATGTAGTTTGAAGGCATG+TGG	0.572855	tig0024227:+31051	None:intergenic
AACTTCCTGTTCATCCGAAT+CGG	0.481907	tig0024227:+31757	None:intergenic
AAGAGAACTGGTGATCTTTA+TGG	0.249179	tig0024227:-28373	Msa1415460:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATCACGAGTAAATTGTAAT+TGG	-	tig0024227:26570-26589	Msa1415460:three_prime_UTR	30.0%
TCACGAGTAAATTGTAATTG+GGG	-	tig0024227:26572-26591	Msa1415460:three_prime_UTR	30.0%
GACAACATTATAGAGAGTTT+AGG	-	tig0024227:26885-26904	Msa1415460:intron	30.0%
AGCCAAAAATCAAATAATCC+AGG	+	tig0024227:27509-27528	None:intergenic	30.0%
AATTCTCATGAAACATGTTC+CGG	-	tig0024227:28417-28436	Msa1415460:intron	30.0%
AATTAGAATACCCTTCTATC+CGG	+	tig0024227:28439-28458	None:intergenic	30.0%
TAGAAAAGGTCACAATTATG+TGG	+	tig0024227:28462-28481	None:intergenic	30.0%
ATGTGGAAAGTGAAATGATA+AGG	+	tig0024227:28663-28682	None:intergenic	30.0%
AAAATACAAGTTAGACAAGG+TGG	+	tig0024227:28872-28891	None:intergenic	30.0%
CACAAAATACAAGTTAGACA+AGG	+	tig0024227:28875-28894	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024227	gene	26169	33581	26169	ID=Msa1415460;Name=Msa1415460
tig0024227	mRNA	26169	33581	26169	ID=Msa1415460-mRNA-1;Parent=Msa1415460;Name=Msa1415460-mRNA-1;_AED=0.09;_eAED=0.09;_QI=222\|1\|1\|1\|1\|1\|13\|660\|568
tig0024227	exon	26169	26889	26169	ID=Msa1415460-mRNA-1:exon:22425;Parent=Msa1415460-mRNA-1
tig0024227	exon	27525	27656	27525	ID=Msa1415460-mRNA-1:exon:22424;Parent=Msa1415460-mRNA-1
tig0024227	exon	27856	27977	27856	ID=Msa1415460-mRNA-1:exon:22423;Parent=Msa1415460-mRNA-1
tig0024227	exon	28324	28431	28324	ID=Msa1415460-mRNA-1:exon:22422;Parent=Msa1415460-mRNA-1
tig0024227	exon	28733	28888	28733	ID=Msa1415460-mRNA-1:exon:22421;Parent=Msa1415460-mRNA-1
tig0024227	exon	29718	29896	29718	ID=Msa1415460-mRNA-1:exon:22420;Parent=Msa1415460-mRNA-1
tig0024227	exon	30034	30175	30034	ID=Msa1415460-mRNA-1:exon:22419;Parent=Msa1415460-mRNA-1
tig0024227	exon	30276	30356	30276	ID=Msa1415460-mRNA-1:exon:22418;Parent=Msa1415460-mRNA-1
tig0024227	exon	30472	30597	30472	ID=Msa1415460-mRNA-1:exon:22417;Parent=Msa1415460-mRNA-1
tig0024227	exon	30893	31120	30893	ID=Msa1415460-mRNA-1:exon:22416;Parent=Msa1415460-mRNA-1
tig0024227	exon	31742	31821	31742	ID=Msa1415460-mRNA-1:exon:22415;Parent=Msa1415460-mRNA-1
tig0024227	exon	31976	32177	31976	ID=Msa1415460-mRNA-1:exon:22414;Parent=Msa1415460-mRNA-1
tig0024227	exon	33270	33581	33270	ID=Msa1415460-mRNA-1:exon:22413;Parent=Msa1415460-mRNA-1
tig0024227	five_prime_UTR	33360	33581	33360	ID=Msa1415460-mRNA-1:five_prime_utr;Parent=Msa1415460-mRNA-1
tig0024227	CDS	33270	33359	33270	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	31976	32177	31976	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	31742	31821	31742	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	30893	31120	30893	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	30472	30597	30472	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	30276	30356	30276	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	30034	30175	30034	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	29718	29896	29718	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	28733	28888	28733	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	28324	28431	28324	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	27856	27977	27856	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	27525	27656	27525	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	CDS	26829	26889	26829	ID=Msa1415460-mRNA-1:cds;Parent=Msa1415460-mRNA-1
tig0024227	three_prime_UTR	26169	26828	26169	ID=Msa1415460-mRNA-1:three_prime_utr;Parent=Msa1415460-mRNA-1

Gene Sequence

>Msa1415460

ATGGCGCTGACATCATCTGCGCGTGATTACATAAATCGCATCTTACAAGACATATCTGGCATGAAGGTTCTCATCCTCGATTCTCAAACGGTAAGTATTGTGAGTGTTGTGTATTCACAATCAGAGCTTCTTCAGAAAGAAGTATTTTTAGTAGAGTTGGTTGATTCAATTTCCAAGTCAAGCGAACCAATGTCACATCTCAAGGCAATTTACTTCCTTCGACCTACATCAGAGAATATCCAGTATATGCGTCGTCAGCTGGCTAGTCCTAGATTCGGAGAATATCACCTATTTTTCTCCAACATATTGAAGGACCATCATATTCACTTACTTGCCGATTCGGATGAACAGGAAGTTGTCCAGCAAGTTCAGGAGTACTATGCAGACTTTGTTGCTATTGATCCTTATCATTTCACTTTCCACATGCCTTCAAACTACATTTATATGCTCCCAGCTGTCGTAGATCCTTCAGCATTGCAGCGTTTCAGTGATCGGGTTGTTGAAGGTCTTGCTGCTGTTTTTTTGACATTAAAACGGAGACCAGTTATTAGATATCAAAGAACATCCGACATTGCAAAAAGAATAGCACATGAAGCATCTAAACTGATGTACCAAGAGGAAAGTGGTCTTTTCGATTTTAGACGAATGGAAGTTTCTCCTTTGTTGTTGGTGATTGACAGGAGGGATGATCCTGTAACCCCATTGCTAAATCAATGGACCTATCAGGCTATGGTTCATGAATTGATAGGAATCCAAGACAACAAAGTGGACGTCAAATCCATTGGTAAATTTCCAAAGGATCAGGAGGAGGTTGTTCTGTCATCGGAACAAGATTCCTTTTTCAAAGCTAACATGTATGAGAATTTCGGAGATATTGGTATGAATATCAAACGACTGGTTGATGAATTTCAACAAGTGGCAAAGAGTAATCAGAACATCCAAACAGTAGAGGACATGGCCAAATTTGTTGACAACTACCCTGAGTACAGAAAAATGCATGGGAATGTGACAAAACATGTGACTTTGGTAACAGAAATGAGCAAGATAGTTGAAGAGCGAAAACTTATGTTAGTTTCACAAACAGAACAAGAGTTGGCTTGCAATGGAGGGCAAGGAGCTGCTTTTGAGGCGGTGACTAATCTACTAAACAATGAAAGCATATCAGATATGGACAGACTACGTTTGGTCATGCTATATGCTTTACGATATGAGAAGGAGAGCCCTGTTCAATTAATGCAGCTTTTCAACAAACTTGCTTCCAGATCTGCCAAATACAAACCTGGGCTTGTCCAGTTTCTTTTGAAGCAGGCTGGTGTTGACAAGAGAACTGGTGATCTTTATGGAAATCGAGATCTAATGAACATTGCGCGTAACATGGCTCGTGGACTGAAGGGTGTTGAGAATGTTTACACCCAGCACCAGCCGCTTCTGTTCCAAATCATGGAAAGCATTGTCAAAGGACGGTTGAGAGATGTAGACTACCCATTTGTTGGGAATCACTTTCAACAAGGAAGGCCCCAGGACGTAATTATCTTCATTGTTGGCGGGACAACATATGAAGAGTCACGGTCTGTTGCTCTACAAAATGCCAGTAATACAGGCGTTCGGTTTATACTTGGTGGCTCTTCTGTTCTTAATTCGAAAAGGTTTTTCAGGGACTTGGAAGAAGCTCAGAGGGTAGCTCGTTCTAGCACCTCTGTTGTTTGA

Protein sequence

>Msa1415460

MALTSSARDYINRILQDISGMKVLILDSQTVSIVSVVYSQSELLQKEVFLVELVDSISKSSEPMSHLKAIYFLRPTSENIQYMRRQLASPRFGEYHLFFSNILKDHHIHLLADSDEQEVVQQVQEYYADFVAIDPYHFTFHMPSNYIYMLPAVVDPSALQRFSDRVVEGLAAVFLTLKRRPVIRYQRTSDIAKRIAHEASKLMYQEESGLFDFRRMEVSPLLLVIDRRDDPVTPLLNQWTYQAMVHELIGIQDNKVDVKSIGKFPKDQEEVVLSSEQDSFFKANMYENFGDIGMNIKRLVDEFQQVAKSNQNIQTVEDMAKFVDNYPEYRKMHGNVTKHVTLVTEMSKIVEERKLMLVSQTEQELACNGGQGAAFEAVTNLLNNESISDMDRLRLVMLYALRYEKESPVQLMQLFNKLASRSAKYKPGLVQFLLKQAGVDKRTGDLYGNRDLMNIARNMARGLKGVENVYTQHQPLLFQIMESIVKGRLRDVDYPFVGNHFQQGRPQDVIIFIVGGTTYEESRSVALQNASNTGVRFILGGSSVLNSKRFFRDLEEAQRVARSSTSVV*