Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418060 | sp|Q1A173|RADL6_ARATH | 67.500 | 80 | 22 | 1 | 1 | 80 | 1 | 76 | 4.92e-34 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418060 | G7J732 | 100.000 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 8.38e-53 | 169 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1418060 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1351560 | Msa1418060 | 0.800819 | 1.204891e-48 | -8.615850e-47 |
| Msa1397550 | Msa1418060 | 0.816979 | 4.181806e-52 | -8.615850e-47 |
| Msa1418060 | Msa1433380 | 0.810169 | 1.317278e-50 | -8.615850e-47 |
| Msa0661840 | Msa1418060 | 0.928767 | 1.811490e-92 | -8.615850e-47 |
| Msa0665010 | Msa1418060 | 0.813715 | 2.224053e-51 | -8.615850e-47 |
| Msa0835010 | Msa1418060 | 0.802446 | 5.589036e-49 | -8.615850e-47 |
| Msa0136520 | Msa1418060 | 0.814298 | 1.654649e-51 | -8.615850e-47 |
| Msa0198140 | Msa1418060 | 0.812597 | 3.913808e-51 | -8.615850e-47 |
| Msa0198220 | Msa1418060 | 0.807062 | 6.071706e-50 | -8.615850e-47 |
| Msa0226580 | Msa1418060 | 0.802443 | 5.596614e-49 | -8.615850e-47 |
| Msa0365200 | Msa1418060 | 0.805701 | 1.175586e-49 | -8.615850e-47 |
| Msa0386790 | Msa1418060 | 0.961003 | 3.355033e-119 | -8.615850e-47 |
| Msa0278340 | Msa1418060 | 0.803772 | 2.972353e-49 | -8.615850e-47 |
| Msa0282880 | Msa1418060 | 0.803649 | 3.152214e-49 | -8.615850e-47 |
| Msa1203720 | Msa1418060 | 0.800389 | 1.474771e-48 | -8.615850e-47 |
| Msa0522830 | Msa1418060 | 0.800175 | 1.630292e-48 | -8.615850e-47 |
| Msa0523830 | Msa1418060 | 0.937906 | 1.615482e-98 | -8.615850e-47 |
| Msa0587060 | Msa1418060 | 0.812390 | 4.343637e-51 | -8.615850e-47 |
| Msa0938190 | Msa1418060 | 0.800910 | 1.154577e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418060 | MtrunA17_Chr3g0144631 | 100.000 | 81 | 0 | 0 | 1 | 81 | 1 | 81 | 1.61e-56 | 169 |
| Msa1418060 | MtrunA17_Chr1g0155181 | 71.014 | 69 | 20 | 0 | 14 | 82 | 5 | 73 | 1.80e-34 | 113 |
| Msa1418060 | MtrunA17_Chr3g0141601 | 69.565 | 69 | 21 | 0 | 14 | 82 | 5 | 73 | 7.05e-32 | 107 |
| Msa1418060 | MtrunA17_Chr7g0255841 | 72.308 | 65 | 18 | 0 | 19 | 83 | 15 | 79 | 1.70e-30 | 103 |
| Msa1418060 | MtrunA17_Chr3g0081631 | 62.963 | 81 | 23 | 2 | 1 | 80 | 1 | 75 | 6.13e-29 | 99.8 |
| Msa1418060 | MtrunA17_Chr8g0373131 | 62.687 | 67 | 24 | 1 | 16 | 82 | 5 | 70 | 2.39e-27 | 95.1 |
| Msa1418060 | MtrunA17_Chr8g0373111 | 62.687 | 67 | 24 | 1 | 16 | 82 | 5 | 70 | 2.43e-27 | 95.1 |
| Msa1418060 | MtrunA17_Chr8g0373121 | 62.687 | 67 | 24 | 1 | 16 | 82 | 5 | 70 | 2.43e-27 | 95.1 |
| Msa1418060 | MtrunA17_Chr5g0404101 | 60.938 | 64 | 25 | 0 | 16 | 79 | 19 | 82 | 7.98e-26 | 92.0 |
| Msa1418060 | MtrunA17_Chr6g0449111 | 60.000 | 65 | 26 | 0 | 14 | 78 | 8 | 72 | 1.71e-25 | 90.5 |
| Msa1418060 | MtrunA17_Chr5g0404301 | 68.852 | 61 | 18 | 1 | 16 | 76 | 4 | 63 | 2.05e-25 | 89.7 |
| Msa1418060 | MtrunA17_Chr8g0373101 | 63.492 | 63 | 22 | 1 | 14 | 76 | 3 | 64 | 3.83e-25 | 89.4 |
| Msa1418060 | MtrunA17_Chr3g0141641 | 52.239 | 67 | 32 | 0 | 16 | 82 | 3 | 69 | 8.41e-22 | 81.6 |
| Msa1418060 | MtrunA17_Chr8g0364741 | 45.122 | 82 | 39 | 1 | 16 | 91 | 10 | 91 | 2.68e-21 | 81.3 |
| Msa1418060 | MtrunA17_Chr5g0429521 | 53.623 | 69 | 32 | 0 | 16 | 84 | 29 | 97 | 6.49e-21 | 84.3 |
| Msa1418060 | MtrunA17_Chr8g0365011 | 52.239 | 67 | 32 | 0 | 14 | 80 | 6 | 72 | 2.33e-20 | 82.4 |
| Msa1418060 | MtrunA17_Chr3g0104071 | 56.522 | 69 | 30 | 0 | 16 | 84 | 43 | 111 | 1.56e-19 | 80.5 |
| Msa1418060 | MtrunA17_Chr7g0241761 | 50.000 | 70 | 35 | 0 | 14 | 83 | 33 | 102 | 2.66e-18 | 77.0 |
| Msa1418060 | MtrunA17_Chr5g0436501 | 50.000 | 68 | 34 | 0 | 16 | 83 | 27 | 94 | 4.44e-18 | 76.3 |
| Msa1418060 | MtrunA17_Chr1g0162081 | 45.455 | 77 | 42 | 0 | 14 | 90 | 10 | 86 | 8.36e-18 | 75.9 |
| Msa1418060 | MtrunA17_Chr7g0270711 | 50.000 | 80 | 33 | 2 | 16 | 88 | 11 | 90 | 1.69e-16 | 68.2 |
| Msa1418060 | MtrunA17_Chr1g0194071 | 59.649 | 57 | 22 | 1 | 16 | 71 | 11 | 67 | 7.93e-16 | 66.6 |
| Msa1418060 | MtrunA17_Chr1g0210471 | 47.619 | 63 | 33 | 0 | 16 | 78 | 24 | 86 | 1.14e-15 | 69.3 |
| Msa1418060 | MtrunA17_Chr4g0062741 | 40.000 | 70 | 39 | 1 | 12 | 78 | 4 | 73 | 2.70e-13 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1418060 | AT1G75250.1 | 67.500 | 80 | 22 | 1 | 1 | 80 | 1 | 76 | 3.36e-35 | 117 |
| Msa1418060 | AT1G75250.2 | 67.500 | 80 | 22 | 1 | 1 | 80 | 1 | 76 | 5.01e-35 | 115 |
| Msa1418060 | AT4G39250.1 | 69.697 | 66 | 20 | 0 | 16 | 81 | 14 | 79 | 1.46e-33 | 112 |
| Msa1418060 | AT2G21650.1 | 65.672 | 67 | 23 | 0 | 16 | 82 | 14 | 80 | 2.14e-30 | 103 |
| Msa1418060 | AT1G19510.1 | 71.429 | 63 | 18 | 0 | 19 | 81 | 15 | 77 | 1.68e-29 | 101 |
| Msa1418060 | AT2G18328.1 | 69.841 | 63 | 19 | 0 | 19 | 81 | 14 | 76 | 9.69e-29 | 99.0 |
| Msa1418060 | AT4G36570.1 | 77.273 | 44 | 10 | 0 | 16 | 59 | 12 | 55 | 3.13e-21 | 79.3 |
| Msa1418060 | AT5G05790.2 | 52.941 | 68 | 32 | 0 | 14 | 81 | 29 | 96 | 6.05e-18 | 76.3 |
| Msa1418060 | AT5G05790.1 | 52.941 | 68 | 32 | 0 | 14 | 81 | 29 | 96 | 6.05e-18 | 76.3 |
| Msa1418060 | AT2G38090.1 | 53.846 | 65 | 30 | 0 | 16 | 80 | 29 | 93 | 1.46e-17 | 75.5 |
| Msa1418060 | AT5G58900.1 | 49.231 | 65 | 33 | 0 | 14 | 78 | 32 | 96 | 2.53e-17 | 74.7 |
| Msa1418060 | AT3G11280.2 | 47.826 | 69 | 36 | 0 | 16 | 84 | 33 | 101 | 4.83e-17 | 73.6 |
| Msa1418060 | AT3G11280.1 | 47.826 | 69 | 36 | 0 | 16 | 84 | 33 | 101 | 4.83e-17 | 73.6 |
| Msa1418060 | AT5G08520.1 | 49.254 | 67 | 34 | 0 | 14 | 80 | 10 | 76 | 3.80e-16 | 71.6 |
| Msa1418060 | AT5G04760.1 | 41.791 | 67 | 38 | 1 | 12 | 78 | 2 | 67 | 3.23e-13 | 62.8 |
| Msa1418060 | AT1G49010.1 | 42.647 | 68 | 36 | 1 | 14 | 78 | 6 | 73 | 1.58e-12 | 61.6 |
| Msa1418060 | AT5G01200.1 | 43.284 | 67 | 37 | 1 | 13 | 78 | 27 | 93 | 3.84e-12 | 60.5 |
| Msa1418060 | AT5G23650.1 | 39.189 | 74 | 45 | 0 | 5 | 78 | 2 | 75 | 3.52e-11 | 58.2 |
Find 23 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCACGTGGATTTGTAATT+TGG | 0.099924 | tig0024534:-24432 | None:intergenic |
| ACAAGCCTTGTGATTCAATT+TGG | 0.187124 | tig0024534:+24232 | Msa1418060:CDS |
| GTGGATTTGTAATTTGGAAT+AGG | 0.364725 | tig0024534:-24426 | None:intergenic |
| TCAAAATTACAAAATCAGAT+AGG | 0.377429 | tig0024534:-24705 | None:intergenic |
| CTTATATAGATGCTTGCCTC+AGG | 0.419615 | tig0024534:+24614 | Msa1418060:intron |
| AGCACTTGCAAAATTTGATA+AGG | 0.422428 | tig0024534:+24284 | Msa1418060:CDS |
| CTGAAGTATCTAAAGTTGAA+TGG | 0.424280 | tig0024534:+24640 | Msa1418060:CDS |
| CACAAGGCTTGTGTTTGCTA+AGG | 0.474591 | tig0024534:-24221 | None:intergenic |
| CAAAATTACAAAATCAGATA+GGG | 0.481920 | tig0024534:-24704 | None:intergenic |
| GATCTCAAACACATTGAGTC+TGG | 0.498892 | tig0024534:+24396 | Msa1418060:CDS |
| AGACAGATGGAGGAACAAAC+AGG | 0.503947 | tig0024534:-24681 | None:intergenic |
| GAGACACTATGAGCTACTCT+TGG | 0.504426 | tig0024534:+24371 | Msa1418060:CDS |
| ACAAGGCTTGTGTTTGCTAA+GGG | 0.544579 | tig0024534:-24220 | None:intergenic |
| ACACTATGAGCTACTCTTGG+AGG | 0.567606 | tig0024534:+24374 | Msa1418060:CDS |
| GTGTTTGCTAAGGGAACTTG+AGG | 0.586339 | tig0024534:-24211 | None:intergenic |
| AATGTAGCAAAAGCAGTTGG+TGG | 0.597235 | tig0024534:+24327 | Msa1418060:CDS |
| CAAAATGTAGCAAAAGCAGT+TGG | 0.599555 | tig0024534:+24324 | Msa1418060:CDS |
| GGAGTCCAAATTGAATCACA+AGG | 0.603739 | tig0024534:-24237 | None:intergenic |
| ATAAGGATACCCCTGACCGT+TGG | 0.605106 | tig0024534:+24301 | Msa1418060:CDS |
| AAATCAGATAGGGAGACAGA+TGG | 0.611076 | tig0024534:-24694 | None:intergenic |
| AGTTGGTGGTAAATCAGTTG+AGG | 0.635333 | tig0024534:+24341 | Msa1418060:CDS |
| TTAGATACTTCAGAAGCCTG+AGG | 0.659984 | tig0024534:-24630 | None:intergenic |
| TCAGATAGGGAGACAGATGG+AGG | 0.662436 | tig0024534:-24691 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAAAAAAAATATCATA+AGG | + | tig0024534:24479-24498 | Msa1418060:intron | 10.0% |
| !!! | TCAAACAACTTGTTTTGTTT+TGG | - | tig0024534:24261-24280 | None:intergenic | 25.0% |
| ! | GAAACATGAACAAAAAATAG+TGG | - | tig0024534:24550-24569 | None:intergenic | 25.0% |
| AGCACTTGCAAAATTTGATA+AGG | + | tig0024534:24284-24303 | Msa1418060:CDS | 30.0% | |
| ! | GTGGATTTGTAATTTGGAAT+AGG | - | tig0024534:24429-24448 | None:intergenic | 30.0% |
| ATGAACAAAAAATAGTGGCA+TGG | - | tig0024534:24545-24564 | None:intergenic | 30.0% | |
| ! | CTGAAGTATCTAAAGTTGAA+TGG | + | tig0024534:24640-24659 | Msa1418060:CDS | 30.0% |
| ! | ACAAGCCTTGTGATTCAATT+TGG | + | tig0024534:24232-24251 | Msa1418060:CDS | 35.0% |
| ! | CTTTTGCTACATTTTGCCAA+CGG | - | tig0024534:24320-24339 | None:intergenic | 35.0% |
| CAAAATGTAGCAAAAGCAGT+TGG | + | tig0024534:24324-24343 | Msa1418060:CDS | 35.0% | |
| CTTCACGTGGATTTGTAATT+TGG | - | tig0024534:24435-24454 | None:intergenic | 35.0% | |
| TAGTGGCATGGTACATAATT+TGG | - | tig0024534:24533-24552 | None:intergenic | 35.0% | |
| ! | ACAAGGCTTGTGTTTGCTAA+GGG | - | tig0024534:24223-24242 | None:intergenic | 40.0% |
| GGAGTCCAAATTGAATCACA+AGG | - | tig0024534:24240-24259 | None:intergenic | 40.0% | |
| AATGTAGCAAAAGCAGTTGG+TGG | + | tig0024534:24327-24346 | Msa1418060:CDS | 40.0% | |
| !! | AGTTGGTGGTAAATCAGTTG+AGG | + | tig0024534:24341-24360 | Msa1418060:CDS | 40.0% |
| GATCTCAAACACATTGAGTC+TGG | + | tig0024534:24396-24415 | Msa1418060:CDS | 40.0% | |
| !! | CTAATTTGCTACACTTCACG+TGG | - | tig0024534:24448-24467 | None:intergenic | 40.0% |
| CTTATATAGATGCTTGCCTC+AGG | + | tig0024534:24614-24633 | Msa1418060:intron | 40.0% | |
| TTAGATACTTCAGAAGCCTG+AGG | - | tig0024534:24633-24652 | None:intergenic | 40.0% | |
| AAATCAGATAGGGAGACAGA+TGG | - | tig0024534:24697-24716 | None:intergenic | 40.0% | |
| ! | GTGTTTGCTAAGGGAACTTG+AGG | - | tig0024534:24214-24233 | None:intergenic | 45.0% |
| ! | CACAAGGCTTGTGTTTGCTA+AGG | - | tig0024534:24224-24243 | None:intergenic | 45.0% |
| ! | TACATTTTGCCAACGGTCAG+GGG | - | tig0024534:24313-24332 | None:intergenic | 45.0% |
| ! | CTACATTTTGCCAACGGTCA+GGG | - | tig0024534:24314-24333 | None:intergenic | 45.0% |
| GAGACACTATGAGCTACTCT+TGG | + | tig0024534:24371-24390 | Msa1418060:CDS | 45.0% | |
| ACACTATGAGCTACTCTTGG+AGG | + | tig0024534:24374-24393 | Msa1418060:CDS | 45.0% | |
| AGACAGATGGAGGAACAAAC+AGG | - | tig0024534:24684-24703 | None:intergenic | 45.0% | |
| ATAAGGATACCCCTGACCGT+TGG | + | tig0024534:24301-24320 | Msa1418060:CDS | 50.0% | |
| ! | GCTACATTTTGCCAACGGTC+AGG | - | tig0024534:24315-24334 | None:intergenic | 50.0% |
| TCAGATAGGGAGACAGATGG+AGG | - | tig0024534:24694-24713 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0024534 | gene | 24207 | 24718 | 24207 | ID=Msa1418060;Name=Msa1418060 |
| tig0024534 | mRNA | 24207 | 24718 | 24207 | ID=Msa1418060-mRNA-1;Parent=Msa1418060;Name=Msa1418060-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0|0.5|1|1|1|2|50|95 |
| tig0024534 | exon | 24207 | 24448 | 24207 | ID=Msa1418060-mRNA-1:exon:179;Parent=Msa1418060-mRNA-1 |
| tig0024534 | exon | 24623 | 24718 | 24623 | ID=Msa1418060-mRNA-1:exon:180;Parent=Msa1418060-mRNA-1 |
| tig0024534 | CDS | 24207 | 24448 | 24207 | ID=Msa1418060-mRNA-1:cds;Parent=Msa1418060-mRNA-1 |
| tig0024534 | CDS | 24623 | 24668 | 24623 | ID=Msa1418060-mRNA-1:cds;Parent=Msa1418060-mRNA-1 |
| tig0024534 | three_prime_UTR | 24669 | 24718 | 24669 | ID=Msa1418060-mRNA-1:three_prime_utr;Parent=Msa1418060-mRNA-1 |
| Gene Sequence |
| Protein sequence |
