AlfalfaGEDB | Msa1421270

M. sativa cultivar zhongmu-4 / Msa1421270

KEGG: K10352
GO: -
PFAM: PF19220.3

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1421270	A0A396JHJ4	52.995	1102	328	18	17	959	7	1077	0.0	849

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0203160	Msa1421270	0.804358	2.244823e-49	-8.615850e-47
Msa0219190	Msa1421270	0.803071	4.153243e-49	-8.615850e-47
Msa0221090	Msa1421270	0.822004	2.986762e-53	-8.615850e-47
Msa0262560	Msa1421270	0.879937	8.287912e-70	-8.615850e-47
Msa0338140	Msa1421270	0.904214	1.550644e-79	-8.615850e-47
Msa0418200	Msa1421270	0.800510	1.393345e-48	-8.615850e-47
Msa0517330	Msa1421270	0.802298	5.995218e-49	-8.615850e-47
Msa0737190	Msa1421270	0.839006	2.050728e-57	-8.615850e-47
Msa0739430	Msa1421270	0.807792	4.251727e-50	-8.615850e-47
Msa0741900	Msa1421270	0.802915	4.473021e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1421270	MtrunA17_Chr2g0325361	52.995	1102	328	18	17	959	7	1077	0.0	849
Msa1421270	MtrunA17_Chr2g0325661	48.518	1012	331	17	2	959	1	876	0.0	690
Msa1421270	MtrunA17_Chr2g0325321	50.859	873	251	14	31	859	23	761	0.0	620
Msa1421270	MtrunA17_Chr2g0325231	45.445	933	404	17	1	874	1	887	0.0	586
Msa1421270	MtrunA17_Chr2g0325261	85.621	306	44	0	1	306	1	306	7.47e-171	499
Msa1421270	MtrunA17_Chr2g0325261	41.935	217	106	7	220	428	98	302	6.00e-21	94.7
Msa1421270	MtrunA17_Chr2g0325261	38.785	214	121	3	376	586	100	306	1.99e-20	93.2
Msa1421270	MtrunA17_Chr2g0325341	85.621	306	44	0	1	306	1	306	7.47e-171	499
Msa1421270	MtrunA17_Chr2g0325341	41.935	217	106	7	220	428	98	302	6.00e-21	94.7
Msa1421270	MtrunA17_Chr2g0325341	38.785	214	121	3	376	586	100	306	1.99e-20	93.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1421270	AT5G48385.1	28.485	165	111	2	798	959	296	456	3.95e-12	70.5
Msa1421270	AT5G27220.4	25.819	763	460	21	124	853	111	800	9.48e-12	69.7
Msa1421270	AT5G27220.2	25.819	763	460	21	124	853	111	800	9.48e-12	69.7
Msa1421270	AT5G27220.1	25.819	763	460	21	124	853	111	800	2.18e-11	68.6
Msa1421270	AT5G27220.3	25.819	763	460	21	124	853	111	800	2.18e-11	68.6
Msa1421270	AT5G16320.1	27.041	196	137	4	766	959	170	361	6.04e-11	66.6

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Find 159 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAGCATTTCAAATAT+TGG	0.196470	tig0024922:+22811	Msa1421270:CDS
AAAAGTTACTGAATGTATTT+TGG	0.139554	tig0024922:+22053	Msa1421270:CDS
AAAAGTTGAAATGAAGGAAG+AGG	0.482754	tig0024922:+22011	Msa1421270:CDS
AAAATTGCCGTCGAGCTGTT+TGG	0.394857	tig0024922:+25134	Msa1421270:CDS
AAACTCATGATACATTTAAC+AGG	0.347965	tig0024922:+26235	Msa1421270:three_prime_UTR
AAACTCTGTTTGCAAAACCC+AGG	0.606810	tig0024922:-25160	None:intergenic
AAAGAAGCATTTCAAATATT+GGG	0.293336	tig0024922:+22812	Msa1421270:CDS
AAATACTGAAAATTCTTTGG+CGG	0.480429	tig0024922:+25016	Msa1421270:CDS
AAATGAAGGAAGAGGAACTC+AGG	0.538991	tig0024922:+22019	Msa1421270:CDS
AACACAAGCGAGAAAACTGA+CGG	0.647061	tig0024922:+24747	Msa1421270:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCTTAACAGAAACATCCA+AGG	-	tig0024922:21710-21729	None:intergenic	30.0%
CTCTTCAATATCTTTCTTGA+TGG	-	tig0024922:21920-21939	None:intergenic	30.0%
AAAAGTTGAAATGAAGGAAG+AGG	+	tig0024922:22011-22030	Msa1421270:CDS	30.0%
CAGAAAAATTAGAGTCAACA+AGG	+	tig0024922:22133-22152	Msa1421270:CDS	30.0%
TCAAAGCAGAAGCAAATTAA+AGG	+	tig0024922:22219-22238	Msa1421270:CDS	30.0%
ACAATTTGAATCAAAGGAGA+AGG	+	tig0024922:22377-22396	Msa1421270:CDS	30.0%
TCAAAAGAGAAGAATTCCAA+AGG	+	tig0024922:22597-22616	Msa1421270:CDS	30.0%
AGAATTTGAATCAAAGGAGA+AGG	+	tig0024922:22755-22774	Msa1421270:CDS	30.0%
TTAAAAGAGAAGCAACTTGA+GGG	+	tig0024922:22975-22994	Msa1421270:CDS	30.0%
TCAAAGGAGAAGAAATTTGA+AGG	+	tig0024922:23017-23036	Msa1421270:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0024922	gene	21541	26268	21541	ID=Msa1421270;Name=Msa1421270
tig0024922	mRNA	21541	26268	21541	ID=Msa1421270-mRNA-1;Parent=Msa1421270;Name=Msa1421270-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0\|1\|0.5\|1\|1\|1\|4\|163\|960
tig0024922	exon	21541	23611	21541	ID=Msa1421270-mRNA-1:exon:2007;Parent=Msa1421270-mRNA-1
tig0024922	exon	24688	25185	24688	ID=Msa1421270-mRNA-1:exon:2008;Parent=Msa1421270-mRNA-1
tig0024922	exon	25681	25853	25681	ID=Msa1421270-mRNA-1:exon:2009;Parent=Msa1421270-mRNA-1
tig0024922	exon	25965	26268	25965	ID=Msa1421270-mRNA-1:exon:2010;Parent=Msa1421270-mRNA-1
tig0024922	CDS	21541	23611	21541	ID=Msa1421270-mRNA-1:cds;Parent=Msa1421270-mRNA-1
tig0024922	CDS	24688	25185	24688	ID=Msa1421270-mRNA-1:cds;Parent=Msa1421270-mRNA-1
tig0024922	CDS	25681	25853	25681	ID=Msa1421270-mRNA-1:cds;Parent=Msa1421270-mRNA-1
tig0024922	CDS	25965	26105	25965	ID=Msa1421270-mRNA-1:cds;Parent=Msa1421270-mRNA-1
tig0024922	three_prime_UTR	26106	26268	26106	ID=Msa1421270-mRNA-1:three_prime_utr;Parent=Msa1421270-mRNA-1

Gene Sequence

>Msa1421270

ATGATGGATGTTGAGGCTTCTTATACGCCGAAAATTTGTGGTGGTGATGACGATGTGAAGAAGGAACCTGCTTCTTGTCAAGACTTTGATTCAAGTTTGAGAACATGTAGTAGTAAATGTGATACTGATGCACTGCCAGTTAAGAGGCTGTTTGATAACAGCAGTTCCTTGGATGTTTCTGTTAAGAAATCAAAGGCGTCGCCGTATGATGATCAGGGAAAAGATGGAGATGGTATTCTGCCGACAAGTACAACTAAAAAGCCGAGCAAATCAGCTGATGAATCATTTTCCTCGCTGATGAAAGAACTTCAATTGGTACAAAAGTCGTTTAAGAAGTGCAAAAGGAAGAGGCAAGTGGAAAAAGGGAGATTGCAGTCCATCAAGAAAGATATTGAAGAGTGCAGCAAAGAGCTTGAAGATAAGAACCAGCTAGTTAGCTGTGTTAATGAAATTCACGACAAAATGCAGGAAAAAGTTGAAATGAAGGAAGAGGAACTCAGGGCGCTTTCCCAAAAAGTTACTGAATGTATTTTGGAACTTAAGACCAAAGAGGAAGATCTTGATGCAGTGAACAAATTAATTAGTGAAGAAACAGAAAAATTAGAGTCAACAAGGAAGAAGTTGATAAATATAAGATCAGAAATGAAGAATAGCTGTGCTTTAATGAAGGAATTTGAGTCAAAGCAGAAGCAAATTAAAGGTCGGGTTAAGGAACTCGAGTCAAAAGAGAAGCAGTGTGAAGGACGAGTGGAGGAGCTTCAGTCAAAGGAGAAGGACTTTGAGGAAAGGGTAAAGAAGCTAGAGTCAAGAGAGAAGCAGCTTGAAGTCCATGTGAAACAATTTGAATCAAAGGAGAAGGAACTTGAAGGTCATATGAATGAGCTTCAATCAGAAAACCAACATTTCAAAAATCGGGTGGAGGAGCTTGATGCAAAAGAGAGGCAAGTTGAAGGACGGGCAATGGAGCTAGAGACAAAAGAGATGCAACATAAAGACCAGGTGAAGGTGTTTGAATCAAAAGAGAAGCATTTCAAAAACCGAATGAAGGAGCTTGAGTCAAAAGAGAAGAATTCCAAAGGTCAGGTGAAGGAGTTGGAATTTAAAAAGAAACGCATTGAAAGGCAAATGAAGGAGCTTGAGTCAAAAGAGAAGCACTTTGAGGAACGGGTAAAGAAGCTGGAGTCAAGAGAGAGGCAGTTTGAAGTCCGTGTGAAAGAATTTGAATCAAAGGAGAAGGTACTTGAAGGTCATACGAATGAGCTTGAATCAAAAAAGAAGCATTTCAAATATTGGGTGAAGGAGCATGATGCAAAAGTAAGGCAAGTTGAAGGACGAGCAATGGAGCTAGAGACAAAAGAGATGCAACATAAAGACCAGGTGAAGGTGTTTGAATCAAAAGAGAAGGATTTTGAGTGCCAAATGAAGGAGCTTCAGTTAAAAGAGAAGCAACTTGAGGGCCGATTGATGGAGCATGAGTCAAAGGAGAAGAAATTTGAAGGCCGTATGAAGGAGATGGAATCTGAAGAGAAGCATGTTAAAAGCATGGTGGAGGAGCTCAAATCAAAATTGAGGCCATTGAAAGGACAACTTCAAGAACTTGACTCTAAAGAGAAGCAACTTGATGTCCAAGTGAAGGAGCTGGAATCTAAAAACAGACACTTTGAAAGACAAGTGAAGGAGTTTGAATCAGAAAAGACGGAATTTGAAGGCCAAATGATGGACTTGCTATTAAAAGAGAAGCATGTTGAAAACCAAATGAAGGAGCTTGAGTCAAAAATGGAGAAATTTGAAGAACAAGTTAAATCGAAGGAGAAAGAACTCGAATCTAAAAATAAGCATTTTGAAAGACAGGTTGAGGACTTCAAATCAAAACAGAAACAAATTGAAGGACGGTTGAATGAACTGGAGTCGGAAGAGTATAAGTTCAAAGTAAAGGTGAAGGAGCTCAACTTAAAAGAAAAGCAGTTTGAAGGACAAGTCAAGGATCCTAAGTCAAAGCTGAATAAATTTGATGGACAACCAAAGGAGCCTGAGTCAAGGAAAAAATATATTGACGAGAAAAAAGAATCCGTTGCATCTTACATGCATGATCAATCAAGTCGTGCCTTTGGTGGAACAAGTTTGCAATTGAACACAAGCGAGAAAACTGACGGAGTTCAATCTCTTTACAATGGTATTTTAGTTAATCTGCAAGAATCATCAGATCCATCAAAACTTGTTTTGGAAATGATACTGAACCCCATCACTCCACTTTGTCAAAAGGGAGATAATGTTGTGATTATTGCTGATTACCAAATCTATCTATTAGAGCAACTGATGAGAATCTCACCAGTTATTGAACCTTGTGTGAGAAAAAAAGCATTGAAGCTAGCATTTGATTTGAAGGCCAAGATGAAAGAAAATACTGAAAATTCTTTGGCGGTTCTTGGTTTTCTACTGCTTTTGTCAATTTATAAATTGCTTGATTCGTTTGATAAAGATGAAGTTTTCGAGCTTTTGGCATTTGTTGCTCTGCACAAAATTGCCGTCGAGCTGTTTGGAAGCCTGGGTTTTGCAAACAGAGTTTCTGATTTTGTTAAGCATCTTATCAAGAGGAAGCAAACTGTTGCAGCTGTTAAATTCAGTTTTGCATATGACTTGGATGACAAGGATCATTTAGTTGATATGTTGCGAGAATACGTCAAAAATGCCAAACTAATTTGTGAGAGCAGTTGCAAGAAATCAAACTCCATTGAAATCAAGGATAAGGCCAGAGATGAAGAAATTGCTAGTATTGGGACTGTTCTGCAATGCATTTCTGACAGCAACCTTGAATCTACGGGTCTGCTTCATAAGGAGATTGAATATCGTATTCTTGAGCTAAAAGCACATAAAGGCTATTAG

Protein sequence

>Msa1421270

MMDVEASYTPKICGGDDDVKKEPASCQDFDSSLRTCSSKCDTDALPVKRLFDNSSSLDVSVKKSKASPYDDQGKDGDGILPTSTTKKPSKSADESFSSLMKELQLVQKSFKKCKRKRQVEKGRLQSIKKDIEECSKELEDKNQLVSCVNEIHDKMQEKVEMKEEELRALSQKVTECILELKTKEEDLDAVNKLISEETEKLESTRKKLINIRSEMKNSCALMKEFESKQKQIKGRVKELESKEKQCEGRVEELQSKEKDFEERVKKLESREKQLEVHVKQFESKEKELEGHMNELQSENQHFKNRVEELDAKERQVEGRAMELETKEMQHKDQVKVFESKEKHFKNRMKELESKEKNSKGQVKELEFKKKRIERQMKELESKEKHFEERVKKLESRERQFEVRVKEFESKEKVLEGHTNELESKKKHFKYWVKEHDAKVRQVEGRAMELETKEMQHKDQVKVFESKEKDFECQMKELQLKEKQLEGRLMEHESKEKKFEGRMKEMESEEKHVKSMVEELKSKLRPLKGQLQELDSKEKQLDVQVKELESKNRHFERQVKEFESEKTEFEGQMMDLLLKEKHVENQMKELESKMEKFEEQVKSKEKELESKNKHFERQVEDFKSKQKQIEGRLNELESEEYKFKVKVKELNLKEKQFEGQVKDPKSKLNKFDGQPKEPESRKKYIDEKKESVASYMHDQSSRAFGGTSLQLNTSEKTDGVQSLYNGILVNLQESSDPSKLVLEMILNPITPLCQKGDNVVIIADYQIYLLEQLMRISPVIEPCVRKKALKLAFDLKAKMKENTENSLAVLGFLLLLSIYKLLDSFDKDEVFELLAFVALHKIAVELFGSLGFANRVSDFVKHLIKRKQTVAAVKFSFAYDLDDKDHLVDMLREYVKNAKLICESSCKKSNSIEIKDKARDEEIASIGTVLQCISDSNLESTGLLHKEIEYRILELKAHKGY*