AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0221090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221090 MtrunA17_Chr2g0325321 53.317 814 280 12 31 818 23 762 0.0 635
Msa0221090 MtrunA17_Chr2g0325361 59.636 659 188 6 222 817 334 977 0.0 607
Msa0221090 MtrunA17_Chr2g0325361 48.022 733 235 15 17 643 7 699 8.19e-140 441
Msa0221090 MtrunA17_Chr2g0325231 48.358 883 373 16 1 814 1 869 0.0 585
Msa0221090 MtrunA17_Chr2g0325861 43.032 897 356 14 1 817 1 822 6.68e-178 533
Msa0221090 MtrunA17_Chr2g0325261 85.621 306 44 0 1 306 1 306 2.69e-172 499
Msa0221090 MtrunA17_Chr2g0325261 41.558 231 95 8 220 432 98 306 3.28e-23 101
Msa0221090 MtrunA17_Chr2g0325261 58.824 85 35 0 460 544 222 306 1.48e-18 87.4
Msa0221090 MtrunA17_Chr2g0325261 36.744 215 106 9 428 624 96 298 2.36e-12 68.9
Msa0221090 MtrunA17_Chr2g0325341 85.621 306 44 0 1 306 1 306 2.69e-172 499
Msa0221090 MtrunA17_Chr2g0325341 41.558 231 95 8 220 432 98 306 3.28e-23 101
Msa0221090 MtrunA17_Chr2g0325341 58.824 85 35 0 460 544 222 306 1.48e-18 87.4
Msa0221090 MtrunA17_Chr2g0325341 36.744 215 106 9 428 624 96 298 2.36e-12 68.9
Msa0221090 MtrunA17_Chr2g0325651 65.155 419 94 5 1 398 1 388 5.95e-161 473
Msa0221090 MtrunA17_Chr2g0325651 43.299 291 123 7 220 482 112 388 1.40e-37 145
Msa0221090 MtrunA17_Chr2g0325651 39.286 252 120 6 369 595 142 385 2.14e-23 103
Msa0221090 MtrunA17_Chr2g0325651 35.354 198 100 3 475 648 178 371 3.67e-13 72.0
Msa0221090 MtrunA17_Chr5g0434741 73.354 319 68 3 2 318 1 304 5.02e-143 424
Msa0221090 MtrunA17_Chr5g0434741 40.553 217 111 5 220 429 98 303 8.97e-20 90.9
Msa0221090 MtrunA17_Chr5g0434741 35.326 184 90 5 197 355 120 299 1.53e-12 69.3
Msa0221090 MtrunA17_Chr5g0434741 35.938 192 106 7 368 550 115 298 4.14e-11 65.1
Msa0221090 MtrunA17_Chr2g0325661 47.994 623 263 9 249 817 161 776 4.18e-134 420
Msa0221090 MtrunA17_Chr2g0325661 47.547 591 241 7 2 530 1 584 6.99e-124 393
Msa0221090 MtrunA17_Chr2g0325661 35.922 309 145 5 149 429 312 595 1.15e-22 104
Msa0221090 MtrunA17_Chr2g0325811 40.544 772 336 14 44 810 79 732 3.93e-127 401
Msa0221090 MtrunA17_Chr2g0325711 37.824 809 374 14 31 817 36 737 6.80e-123 389
Msa0221090 MtrunA17_Chr2g0325761 43.994 641 249 11 2 542 1 631 8.39e-120 377
Msa0221090 MtrunA17_Chr2g0325691 44.274 716 314 13 2 640 1 708 2.54e-117 372
Msa0221090 MtrunA17_Chr2g0325691 42.535 355 184 3 186 520 364 718 5.28e-48 182
Msa0221090 MtrunA17_Chr2g0325691 51.883 239 108 2 405 643 226 457 3.57e-42 164
Msa0221090 MtrunA17_Chr2g0325691 39.750 400 190 7 124 477 323 717 4.54e-40 158
Msa0221090 MtrunA17_Chr2g0325691 35.674 356 171 8 91 435 409 717 1.97e-20 96.7
Msa0221090 MtrunA17_Chr2g0325691 36.396 283 137 9 377 643 118 373 5.36e-14 76.3
Msa0221090 MtrunA17_Chr2g0325281 44.444 639 279 14 31 653 23 601 8.94e-116 364
Msa0221090 MtrunA17_Chr2g0325351 50.816 490 202 8 341 817 125 588 1.06e-114 364
Msa0221090 MtrunA17_Chr2g0325351 42.227 476 181 13 1 443 1 415 7.18e-68 238
Msa0221090 MtrunA17_Chr2g0325611 57.105 380 129 5 445 817 138 490 5.96e-110 348
Msa0221090 MtrunA17_Chr2g0325611 43.701 254 103 5 139 373 77 309 2.19e-35 142
Msa0221090 MtrunA17_Chr2g0325611 38.683 243 120 6 261 500 94 310 1.47e-20 96.7
Msa0221090 MtrunA17_Chr2g0325611 39.884 173 78 3 279 425 147 319 5.32e-18 88.6
Msa0221090 MtrunA17_Chr2g0325611 30.263 304 178 10 39 322 38 327 2.70e-13 73.6
Msa0221090 MtrunA17_Chr6g0471251 48.356 517 208 9 349 817 162 667 5.40e-108 348
Msa0221090 MtrunA17_Chr6g0471251 45.972 509 188 8 1 444 1 487 2.16e-91 304
Msa0221090 MtrunA17_Chr6g0471251 37.952 332 174 4 223 528 162 487 6.11e-30 127
Msa0221090 MtrunA17_Chr2g0280141 36.730 795 364 19 39 817 47 718 1.13e-100 330
Msa0221090 MtrunA17_Chr2g0325621 48.211 475 189 9 377 817 110 561 3.39e-96 314
Msa0221090 MtrunA17_Chr2g0325621 38.747 431 164 11 9 387 4 386 2.91e-45 173
Msa0221090 MtrunA17_Chr2g0325621 34.853 307 177 7 198 490 80 377 9.22e-22 100
Msa0221090 MtrunA17_Chr2g0325621 31.690 284 168 4 253 530 112 375 1.01e-15 81.6
Msa0221090 MtrunA17_Chr5g0434761 88.272 162 19 0 653 814 1 162 1.26e-92 291
Msa0221090 MtrunA17_Chr2g0325221 45.079 508 222 7 44 499 84 586 2.33e-92 305
Msa0221090 MtrunA17_Chr2g0325221 32.506 403 189 11 253 638 165 501 1.22e-19 94.0
Msa0221090 MtrunA17_Chr2g0325221 43.871 155 83 1 486 640 311 461 1.07e-17 87.8
Msa0221090 MtrunA17_Chr2g0325701 47.059 459 210 8 369 817 157 592 5.15e-92 301
Msa0221090 MtrunA17_Chr2g0325701 38.235 442 203 10 2 402 1 413 1.58e-51 190
Msa0221090 MtrunA17_Chr2g0325701 35.014 357 166 12 211 562 110 405 2.04e-23 105
Msa0221090 MtrunA17_Chr2g0325331 94.194 155 9 0 653 807 1 155 6.20e-92 286
Msa0221090 MtrunA17_Chr2g0325641 60.129 311 86 3 376 648 1 311 7.67e-89 284
Msa0221090 MtrunA17_Chr2g0325641 40.683 322 173 3 252 573 3 306 3.06e-44 162
Msa0221090 MtrunA17_Chr2g0325641 40.523 306 154 3 222 499 1 306 1.02e-40 152
Msa0221090 MtrunA17_Chr2g0325641 41.564 243 139 1 420 659 3 245 3.05e-32 128
Msa0221090 MtrunA17_Chr2g0325641 34.604 341 166 9 124 444 4 307 4.42e-25 107
Msa0221090 MtrunA17_Chr2g0325641 30.795 302 175 7 91 373 20 306 2.08e-15 78.6
Msa0221090 MtrunA17_Chr5g0416271 49.123 342 160 5 489 818 147 486 8.65e-81 268
Msa0221090 MtrunA17_Chr5g0416271 40.625 384 162 6 29 409 12 332 4.94e-55 197
Msa0221090 MtrunA17_Chr5g0416271 36.777 242 130 7 238 477 96 316 5.88e-21 97.4
Msa0221090 MtrunA17_Chr2g0325721 41.199 517 260 9 2 491 1 500 6.63e-75 252
Msa0221090 MtrunA17_Chr2g0325721 34.565 460 219 12 186 638 105 489 1.64e-28 120
Msa0221090 MtrunA17_Chr2g0333341 44.628 363 169 4 461 813 267 607 3.68e-68 238
Msa0221090 MtrunA17_Chr2g0333341 37.824 386 193 9 32 410 104 449 1.54e-43 167
Msa0221090 MtrunA17_Chr2g0333341 31.633 294 157 8 251 530 180 443 7.03e-16 82.0
Msa0221090 MtrunA17_Chr2g0325631 78.395 162 35 0 653 814 1 162 1.03e-67 225
Msa0221090 MtrunA17_Chr2g0332961 46.364 330 128 7 466 787 65 353 7.06e-61 209
Msa0221090 MtrunA17_Chr2g0332961 36.538 260 127 8 44 301 4 227 8.92e-23 100
Msa0221090 MtrunA17_Chr2g0332951 49.180 244 86 4 579 818 2 211 3.06e-57 194
Msa0221090 MtrunA17_Chr5g0434751 51.495 301 121 1 348 648 1 276 1.59e-56 196
Msa0221090 MtrunA17_Chr5g0434751 41.667 264 133 1 222 464 1 264 1.11e-35 137
Msa0221090 MtrunA17_Chr5g0434751 43.243 296 129 5 306 594 1 264 4.87e-34 132
Msa0221090 MtrunA17_Chr5g0434751 43.066 274 135 2 264 530 1 260 7.28e-34 132
Msa0221090 MtrunA17_Chr5g0434751 35.849 265 146 3 176 440 28 268 1.13e-19 90.5
Msa0221090 MtrunA17_Chr5g0434751 33.548 310 153 9 100 398 1 268 5.32e-14 73.6
Msa0221090 MtrunA17_Chr2g0325241 42.988 328 151 4 425 739 120 424 1.99e-53 191
Msa0221090 MtrunA17_Chr2g0325241 39.382 259 116 4 216 472 105 324 5.84e-29 120
Msa0221090 MtrunA17_Chr2g0325241 39.914 233 115 4 300 532 121 328 2.56e-27 115
Msa0221090 MtrunA17_Chr2g0325241 35.220 318 141 9 42 359 71 323 1.18e-19 92.4
Msa0221090 MtrunA17_Chr2g0325771 64.571 175 52 2 646 818 10 176 5.11e-52 179
Msa0221090 MtrunA17_Chr7g0235901 38.000 400 157 7 61 458 1 311 1.51e-50 180
Msa0221090 MtrunA17_Chr7g0235901 53.922 204 78 3 397 588 112 311 1.65e-40 151
Msa0221090 MtrunA17_Chr7g0235901 40.594 202 111 3 433 630 115 311 3.91e-23 100
Msa0221090 MtrunA17_Chr7g0235901 38.000 150 69 3 516 643 93 240 5.64e-11 64.7
Msa0221090 MtrunA17_Chr2g0325781 42.727 330 129 10 2 298 1 303 8.07e-50 177
Msa0221090 MtrunA17_Chr2g0325781 34.562 217 123 5 279 493 103 302 1.14e-15 79.0
Msa0221090 MtrunA17_Chr2g0325781 33.824 204 114 4 223 409 103 302 5.42e-12 67.8
Msa0221090 MtrunA17_Chr3g0140591 56.548 168 72 1 650 817 6 172 4.18e-44 157
Msa0221090 MtrunA17_Chr2g0325741 37.346 324 132 9 1 275 1 302 8.49e-39 146
Msa0221090 MtrunA17_Chr2g0325741 39.752 161 61 7 433 558 146 305 3.36e-11 65.5
Msa0221090 MtrunA17_Chr2g0325741 37.179 156 69 5 253 401 169 302 4.57e-11 65.1
Msa0221090 MtrunA17_Chr2g0325291 57.823 147 62 0 668 814 2 148 1.32e-37 140
Msa0221090 MtrunA17_Chr2g0325751 47.753 178 56 4 498 663 3 155 1.32e-28 112
Msa0221090 MtrunA17_Chr2g0325751 45.312 128 62 2 322 449 9 128 1.83e-16 77.4
Msa0221090 MtrunA17_Chr2g0325751 41.803 122 64 1 343 464 2 116 1.11e-14 72.4
Msa0221090 MtrunA17_Chr2g0325751 39.850 133 73 1 390 522 7 132 4.86e-14 70.5
Msa0221090 MtrunA17_Chr2g0325751 38.849 139 75 2 442 577 3 134 1.62e-13 68.9
Msa0221090 MtrunA17_Chr2g0325751 39.167 120 66 1 217 336 2 114 3.93e-12 65.1
Msa0221090 MtrunA17_Chr2g0325751 39.516 124 68 1 264 387 7 123 4.69e-11 62.0
Msa0221090 MtrunA17_Chr2g0325201 47.134 157 82 1 163 318 72 228 5.21e-26 107
Msa0221090 MtrunA17_Chr2g0325201 37.017 181 99 3 279 444 48 228 1.18e-11 65.5
Msa0221090 MtrunA17_Chr4g0044221 34.783 207 73 4 575 779 168 314 1.68e-24 105
Msa0221090 MtrunA17_Chr8g0361651 34.717 265 155 6 555 814 51 302 4.48e-24 107
Msa0221090 MtrunA17_Chr2g0325801 43.871 155 55 4 1 152 1 126 2.30e-22 93.6
Msa0221090 MtrunA17_Chr2g0325211 39.362 188 91 2 455 642 2 166 3.98e-19 86.3
Msa0221090 MtrunA17_Chr2g0325211 37.342 158 95 1 273 430 2 155 8.02e-15 73.9
Msa0221090 MtrunA17_Chr2g0325211 42.000 150 83 1 328 477 1 146 4.60e-13 68.6
Msa0221090 MtrunA17_Chr2g0325211 36.601 153 87 1 231 373 2 154 3.38e-12 66.2
Msa0221090 MtrunA17_Chr2g0325211 37.580 157 94 1 385 541 2 154 1.06e-11 64.7
Msa0221090 MtrunA17_Chr2g0325211 37.342 158 81 2 315 458 2 155 1.32e-11 64.3
Msa0221090 MtrunA17_Chr2g0325271 92.683 41 3 0 653 693 1 41 6.79e-19 84.0
Msa0221090 MtrunA17_Chr2g0325791 47.414 116 58 1 279 394 44 156 5.26e-18 86.3
Msa0221090 MtrunA17_Chr2g0325791 40.714 140 78 2 307 442 24 162 3.86e-16 80.5
Msa0221090 MtrunA17_Chr2g0325791 37.297 185 99 3 309 493 18 185 5.18e-16 80.1
Msa0221090 MtrunA17_Chr2g0325791 42.857 126 62 1 227 352 41 156 4.25e-15 77.4
Msa0221090 MtrunA17_Chr2g0325791 35.057 174 88 3 374 547 21 169 2.32e-14 75.1
Msa0221090 MtrunA17_Chr2g0325791 43.165 139 77 2 179 316 25 162 2.45e-14 75.1
Msa0221090 MtrunA17_Chr2g0325791 36.735 147 88 2 237 379 24 169 2.14e-13 72.4
Msa0221090 MtrunA17_Chr2g0325791 40.123 162 68 4 433 594 24 156 9.09e-13 70.5
Msa0221090 MtrunA17_Chr2g0325791 37.107 159 89 2 277 435 22 169 9.34e-13 70.5
Msa0221090 MtrunA17_Chr4g0024061 35.503 169 74 3 649 817 419 552 9.21e-18 88.2
Msa0221090 MtrunA17_Chr7g0235921 70.238 84 24 1 731 814 1 83 2.46e-17 78.6
Msa0221090 MtrunA17_Chr2g0325731 58.929 56 23 0 432 487 1 56 1.96e-12 63.2
Msa0221090 MtrunA17_Chr2g0325731 53.448 58 27 0 306 363 1 58 9.93e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221090 AT5G27220.4 25.962 728 461 17 124 811 111 800 2.52e-16 84.3
Msa0221090 AT5G27220.2 25.962 728 461 17 124 811 111 800 2.52e-16 84.3
Msa0221090 AT5G27220.1 25.962 728 461 17 124 811 111 800 2.59e-16 84.3
Msa0221090 AT5G27220.3 25.962 728 461 17 124 811 111 800 2.59e-16 84.3

Find 130 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGTTACTGAATGTATTT+TGG 0.139554 2_1:+69687775 Msa0221090:CDS
AGATCCATCAAAACTTGTTT+TGG 0.235745 2_1:+69690449 Msa0221090:CDS
CGTCGAGCTGTTTGGAAGCC+TGG 0.247994 2_1:+69690773 Msa0221090:CDS
CTCTTCAATATCTTTCTTGA+TGG 0.272283 2_1:-69687639 None:intergenic
AGGAAGAGGCAAGTGGAAAA+AGG 0.278648 2_1:+69687605 Msa0221090:CDS
TTCCTTGACTCAGGCTCCTT+TGG 0.279411 2_1:-69689156 None:intergenic
AGAAAATACTGAAAATTCTT+TGG 0.279753 2_1:+69690644 Msa0221090:CDS
TCAAAGCAGAAGCAATTTAA+AGG 0.293203 2_1:+69687941 Msa0221090:CDS
CTCACACAAGGTTCAATAAC+TGG 0.294449 2_1:-69690576 None:intergenic
GAAAATTCTTTGGCGGTTCT+TGG 0.308827 2_1:+69690654 Msa0221090:CDS
TTGACTCAAGCTCCTTCATT+TGG 0.318173 2_1:-69688888 None:intergenic
CAACTCCTTCACCTGACCTT+TGG 0.322418 2_1:-69688335 None:intergenic
TGCATCAAGATCTTCCTCTT+TGG 0.343028 2_1:-69687807 None:intergenic
TTAATAGCAAGTCCATCATT+TGG 0.381291 2_1:-69688846 None:intergenic
TTAACTGAAGCTCCTTCATT+TGG 0.384104 2_1:-69688552 None:intergenic
TCAAAGCTGAATAAATTTGA+TGG 0.388039 2_1:+69689129 Msa0221090:CDS
GGCGTCCCCGTATGATGATC+AGG 0.388191 2_1:+69687457 Msa0221090:CDS
GAAGCTAGCATTTGATTTGA+AGG 0.391473 2_1:+69690611 Msa0221090:CDS
AAAATTGCCGTCGAGCTGTT+TGG 0.394857 2_1:+69690765 Msa0221090:CDS
GTTGCTCTAATAGATAGATT+TGG 0.397891 2_1:-69690539 None:intergenic
TTATCAAACAGCCTCTTAAC+TGG 0.399033 2_1:-69687398 None:intergenic
AATTTATTCAGCTTTGACTT+AGG 0.402255 2_1:-69689123 None:intergenic
TTGACTCAAGCTCCTTCATT+CGG 0.411679 2_1:-69688300 None:intergenic
TCTTGAAGTTGTCCTTTCAA+TGG 0.423349 2_1:-69688709 None:intergenic
GTCGAGCTGTTTGGAAGCCT+GGG 0.426950 2_1:+69690774 Msa0221090:CDS
GCAACTTGAAGGCCGATTGA+TGG 0.430744 2_1:+69688582 Msa0221090:CDS
CATCACTCCACTTTGTCAAA+AGG 0.431635 2_1:+69690488 Msa0221090:CDS
AGAGAAGCACTTTGAGGAAC+GGG 0.434622 2_1:+69688408 Msa0221090:CDS
GGAGTTTGAATCAGAAAAGA+AGG 0.434930 2_1:+69688813 Msa0221090:CDS
TGAAGGACGGTTGAATGAAC+TGG 0.435099 2_1:+69689020 Msa0221090:CDS
GCAGAAGCAATTTAAAGGTC+GGG 0.438292 2_1:+69687946 Msa0221090:CDS
GTTTGATAACAGCAGTTCCT+TGG 0.438536 2_1:+69687412 Msa0221090:CDS
GATCAATCAAGTCGTGCCTT+TGG 0.443224 2_1:+69690336 Msa0221090:CDS
TTAAAAGAAAAGCAGTTTGA+AGG 0.444691 2_1:+69689087 Msa0221090:CDS
GTCAAAAGAGAAGCACTTTG+AGG 0.449934 2_1:+69688402 Msa0221090:CDS
GCTGATGAAAGAACTTCAAT+TGG 0.452218 2_1:+69687556 Msa0221090:CDS
GCAATTTAAAGGTCGGGTTA+AGG 0.454318 2_1:+69687952 Msa0221090:CDS
GTCAAAGGAGAAGGACTTTG+AGG 0.459831 2_1:+69688024 Msa0221090:CDS
TCTTATACGCCGAAAATTTG+TGG 0.461733 2_1:+69687281 Msa0221090:CDS
TTGACTCATGCTCCATCAAT+CGG 0.462835 2_1:-69688594 None:intergenic
TTAAAAGAGAAGCAACTTGA+AGG 0.467415 2_1:+69688571 Msa0221090:CDS
AGAGAAGAATTCCAAAGGTC+AGG 0.475943 2_1:+69688324 Msa0221090:CDS
TCAAAACAGAAACAATTTGA+AGG 0.477074 2_1:+69689003 Msa0221090:CDS
GAAATTTGAAGGCCGTATGA+AGG 0.478008 2_1:+69688624 Msa0221090:CDS
CTGATGCACTGCCAGTTAAG+AGG 0.478611 2_1:+69687387 Msa0221090:CDS
AAATACTGAAAATTCTTTGG+CGG 0.480429 2_1:+69690647 Msa0221090:CDS
AAAAGTTGAAATGAAGGAAG+AGG 0.482754 2_1:+69687733 Msa0221090:CDS
GATTCATCAGCTGATTTGCT+CGG 0.484084 2_1:-69687524 None:intergenic
ATCACTCCACTTTGTCAAAA+GGG 0.489260 2_1:+69690489 Msa0221090:CDS
TGTGAAACAATTTGAATCAA+AGG 0.491078 2_1:+69688093 Msa0221090:CDS
CAATCAAGTCGTGCCTTTGG+TGG 0.496384 2_1:+69690339 Msa0221090:CDS
GGAATTTGAAGGCCAAATGA+TGG 0.498386 2_1:+69688834 Msa0221090:CDS
GCAAGTTGAAGGACGAGCAA+TGG 0.499141 2_1:+69688204 Msa0221090:CDS
CATTAACACAGCTAACTAGC+TGG 0.500508 2_1:-69687688 None:intergenic
GGAGAAGGACTTTGAGGAAA+GGG 0.500675 2_1:+69688030 Msa0221090:CDS
AGTGGAGGAGCTTCAGTCAA+AGG 0.502204 2_1:+69688009 Msa0221090:CDS
AGCAGAAGCAATTTAAAGGT+CGG 0.502227 2_1:+69687945 Msa0221090:CDS
CAGATTCCATCTCCTTCATA+CGG 0.504321 2_1:-69688636 None:intergenic
TAGATTCCAGCTCCTTCACT+TGG 0.513418 2_1:-69688762 None:intergenic
AGAGATACAACATGAAGGTC+GGG 0.513506 2_1:+69688240 Msa0221090:CDS
TCAGAAAAGAAGGAATTTGA+AGG 0.518263 2_1:+69688823 Msa0221090:CDS
AGTCGTTTAAGAAGTGCAAA+AGG 0.518332 2_1:+69687585 Msa0221090:CDS
TATGATGATCAGGGAAAAGA+TGG 0.519539 2_1:+69687467 Msa0221090:CDS
AGAGTATAAGTTCAAAGTAA+AGG 0.521614 2_1:+69689050 Msa0221090:CDS
TGTGAAAGAATTTGAATCAA+AGG 0.523232 2_1:+69688471 Msa0221090:CDS
GCATGTTAAAAGCGTGGTGG+AGG 0.528650 2_1:+69688666 Msa0221090:CDS
TCAAAGGAGAAGAAATTTGA+AGG 0.531012 2_1:+69688613 Msa0221090:CDS
CAAAGGTCAGGTGAAGGAGT+TGG 0.536905 2_1:+69688336 Msa0221090:CDS
AAATGAAGGAAGAGGAACTC+AGG 0.538991 2_1:+69687741 Msa0221090:CDS
TCAAAGGAGAAGGTACTTGA+AGG 0.540155 2_1:+69688487 Msa0221090:CDS
AGAGTCAACAAGGAAGAAGT+TGG 0.543186 2_1:+69687865 Msa0221090:CDS
GAATAGCTGTGCTTTAATGA+AGG 0.543322 2_1:+69687910 Msa0221090:CDS
TTAAGAAGTGCAAAAGGAAG+AGG 0.543495 2_1:+69687591 Msa0221090:CDS
TGAGGAACGGGTAAAGAAGC+TGG 0.543752 2_1:+69688420 Msa0221090:CDS
TCAAAGTCTTGACAAGAAGC+AGG 0.545616 2_1:-69687329 None:intergenic
TGAAATTCACAACAAAATGC+AGG 0.546544 2_1:+69687709 Msa0221090:CDS
GCAGTGTGAAGGACGAGTGG+AGG 0.548347 2_1:+69687994 Msa0221090:CDS
TCAAAGGAGAAGGAACTTGA+AGG 0.556316 2_1:+69688109 Msa0221090:CDS
TGATGTCCAAGTGAAGGAGC+TGG 0.557364 2_1:+69688756 Msa0221090:CDS
TCAAAATTGAGGCCATTGAA+AGG 0.560208 2_1:+69688697 Msa0221090:CDS
ATTGATGGAGCATGAGTCAA+AGG 0.560494 2_1:+69688597 Msa0221090:CDS
ACAATTTGAATCAAAGGAGA+AGG 0.560708 2_1:+69688099 Msa0221090:CDS
GCAAAAGAGAGGCAAGTTGA+AGG 0.562888 2_1:+69688193 Msa0221090:CDS
TGAAGAACAAGTTAAATCGA+AGG 0.563135 2_1:+69688924 Msa0221090:CDS
ACGCATTGAAAGGCAAATGA+AGG 0.563814 2_1:+69688372 Msa0221090:CDS
AACAGAAACAATTTGAAGGA+CGG 0.564168 2_1:+69689007 Msa0221090:CDS
GCAGTTTGAAGGACAAGTCA+AGG 0.564315 2_1:+69689098 Msa0221090:CDS
AAGAGATACAACATGAAGGT+CGG 0.565165 2_1:+69688239 Msa0221090:CDS
TAAGTTCAAAGTAAAGGTGA+AGG 0.565515 2_1:+69689056 Msa0221090:CDS
AGAATTTGAATCAAAGGAGA+AGG 0.567507 2_1:+69688477 Msa0221090:CDS
GCATGTTGAAAACCAAATGA+AGG 0.570282 2_1:+69688876 Msa0221090:CDS
TTGATTCAAATTGTTTCACA+TGG 0.571176 2_1:-69688090 None:intergenic
GGAGCTTCAGTCAAAGGAGA+AGG 0.571348 2_1:+69688015 Msa0221090:CDS
TAAATTTGATGGACAACCAA+AGG 0.575089 2_1:+69689140 Msa0221090:CDS
TGAAGGCCGTATGAAGGAGA+TGG 0.575488 2_1:+69688630 Msa0221090:CDS
ACGGTTGAATGAACTGGAGT+CGG 0.579242 2_1:+69689026 Msa0221090:CDS
TTGCAAACTTGTTCCACCAA+AGG 0.581513 2_1:-69690352 None:intergenic
GAATTCCAAAGGTCAGGTGA+AGG 0.589794 2_1:+69688330 Msa0221090:CDS
AAGAGAAGCACTTTGAGGAA+CGG 0.589834 2_1:+69688407 Msa0221090:CDS
ACACTTTGAAAGACAAGTGA+AGG 0.589925 2_1:+69688792 Msa0221090:CDS
GAAGCAGTGTGAAGGACGAG+TGG 0.596138 2_1:+69687991 Msa0221090:CDS
GATCAGGGAAAAGATGGAGA+TGG 0.597551 2_1:+69687473 Msa0221090:CDS
TTTCTTAACAGAAACATCCA+AGG 0.599750 2_1:-69687429 None:intergenic
TCAAAAGAGAAGCAGTGTGA+AGG 0.600875 2_1:+69687983 Msa0221090:CDS
AGGAGCTCAAATCAAAATTG+AGG 0.601068 2_1:+69688686 Msa0221090:CDS
ACAACATGAAGGTCGGGTGA+AGG 0.606722 2_1:+69688246 Msa0221090:CDS
AAACTCTGTTTGCAAAACCC+AGG 0.606810 2_1:-69690791 None:intergenic
TGGTGATGACGATGTGAAGA+AGG 0.610285 2_1:+69687304 Msa0221090:CDS
AACCAAAGGAGCCTGAGTCA+AGG 0.611830 2_1:+69689154 Msa0221090:CDS
GCAACTTGATGTCCAAGTGA+AGG 0.617345 2_1:+69688750 Msa0221090:CDS
CTGTTGAATGATGGATGTTG+AGG 0.618487 2_1:+69687256 None:intergenic
TTGATTCAAATTCTTTCACA+CGG 0.620040 2_1:-69688468 None:intergenic
GGAGTTCAATCTCTTTACAA+TGG 0.620269 2_1:+69690399 Msa0221090:CDS
TCAAAAGAGAAGAATTCCAA+AGG 0.620884 2_1:+69688319 Msa0221090:CDS
GTGCAAAAGGAAGAGGCAAG+TGG 0.621092 2_1:+69687598 Msa0221090:CDS
TTGAAGTTCTTTCATCAGCG+AGG 0.621846 2_1:-69687552 None:intergenic
TGTTTCTGTTAAGAAATCAA+AGG 0.621882 2_1:+69687436 Msa0221090:CDS
TTTGACAAAGTGGAGTGATG+GGG 0.623526 2_1:-69690485 None:intergenic
AGAAGCTGGAGTCAAGAGAG+AGG 0.627544 2_1:+69688434 Msa0221090:CDS
AGGAGAAGGACTTTGAGGAA+AGG 0.630256 2_1:+69688029 Msa0221090:CDS
CAGGCTTCCAAACAGCTCGA+CGG 0.630568 2_1:-69690772 None:intergenic
GCGTCCCCGTATGATGATCA+GGG 0.632309 2_1:+69687458 Msa0221090:CDS
ACAAAAGAGATACAACATGA+AGG 0.635717 2_1:+69688235 Msa0221090:CDS
TTTGGAACTTAAGACCAAAG+AGG 0.637114 2_1:+69687793 Msa0221090:CDS
AACACAAGCGAGAAAACTGA+CGG 0.647061 2_1:+69690378 Msa0221090:CDS
TATACGCCGAAAATTTGTGG+TGG 0.652786 2_1:+69687284 Msa0221090:CDS
AATGAAGGAAGAGGAACTCA+GGG 0.659325 2_1:+69687742 Msa0221090:CDS
AGGAGCTTGATGCAAAAGAG+AGG 0.678070 2_1:+69688182 Msa0221090:CDS
AGAGAAGCATGTTAAAAGCG+TGG 0.692605 2_1:+69688660 Msa0221090:CDS
GAAGCATGTTAAAAGCGTGG+TGG 0.701453 2_1:+69688663 Msa0221090:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGGTTTTATTATAATATTA+TGG + chr2_1:69689475-69689494 Msa0221090:intron 10.0%
!! AAAACAAACATTTCAAAAAT+CGG + chr2_1:69688155-69688174 Msa0221090:CDS 15.0%
!! AAAATATCATCATCATTAAA+AGG - chr2_1:69689262-69689281 None:intergenic 15.0%
!!! AATACATTCAGTAACTTTTT+GGG - chr2_1:69687774-69687793 None:intergenic 20.0%
!!! AAATACATTCAGTAACTTTT+TGG - chr2_1:69687775-69687794 None:intergenic 20.0%
!!! AAAAGTTACTGAATGTATTT+TGG + chr2_1:69687775-69687794 Msa0221090:CDS 20.0%
!! AAACAAACATTTCAAAAATC+GGG + chr2_1:69688156-69688175 Msa0221090:CDS 20.0%
!! TTTAAAGAATGAAGTAAATC+AGG - chr2_1:69689291-69689310 None:intergenic 20.0%
!! TCATCAAATTATGAAATAGA+TGG - chr2_1:69689734-69689753 None:intergenic 20.0%
!! TAAATATACTTTCTACTGTT+GGG - chr2_1:69689773-69689792 None:intergenic 20.0%
!! ATAAATATACTTTCTACTGT+TGG - chr2_1:69689774-69689793 None:intergenic 20.0%
!! AAAACTTCGCTAAAATATTT+CGG + chr2_1:69689808-69689827 Msa0221090:intron 20.0%
!!! GTCATATGTTGTAAATTTTA+TGG + chr2_1:69689830-69689849 Msa0221090:intron 20.0%
!! AGAAAATACTGAAAATTCTT+TGG + chr2_1:69690644-69690663 Msa0221090:CDS 20.0%
!! TGTTTCTGTTAAGAAATCAA+AGG + chr2_1:69687436-69687455 Msa0221090:CDS 25.0%
! TTGATTCAAATTGTTTCACA+TGG - chr2_1:69688093-69688112 None:intergenic 25.0%
! TGTGAAACAATTTGAATCAA+AGG + chr2_1:69688093-69688112 Msa0221090:CDS 25.0%
! TTAAAAAGAAACGCATTGAA+AGG + chr2_1:69688362-69688381 Msa0221090:CDS 25.0%
! TTGATTCAAATTCTTTCACA+CGG - chr2_1:69688471-69688490 None:intergenic 25.0%
! TGTGAAAGAATTTGAATCAA+AGG + chr2_1:69688471-69688490 Msa0221090:CDS 25.0%
!!! AAATAAGCATTTTGAAAGAC+AGG + chr2_1:69688966-69688985 Msa0221090:CDS 25.0%
! TCAAAACAGAAACAATTTGA+AGG + chr2_1:69689003-69689022 Msa0221090:CDS 25.0%
! AGAGTATAAGTTCAAAGTAA+AGG + chr2_1:69689050-69689069 Msa0221090:CDS 25.0%
! TTAAAAGAAAAGCAGTTTGA+AGG + chr2_1:69689087-69689106 Msa0221090:CDS 25.0%
!! AATTTATTCAGCTTTGACTT+AGG - chr2_1:69689126-69689145 None:intergenic 25.0%
! TCAAAGCTGAATAAATTTGA+TGG + chr2_1:69689129-69689148 Msa0221090:CDS 25.0%
!!! ATATATTTTTTCCTTGACTC+AGG - chr2_1:69689168-69689187 None:intergenic 25.0%
! GAGATACATAAAATGTTCAA+GGG - chr2_1:69689418-69689437 None:intergenic 25.0%
! AGAGATACATAAAATGTTCA+AGG - chr2_1:69689419-69689438 None:intergenic 25.0%
! TTATAATAAAACCATAGCCA+TGG - chr2_1:69689470-69689489 None:intergenic 25.0%
! AATATTCTGACAAACATTGT+GGG + chr2_1:69689569-69689588 Msa0221090:intron 25.0%
! CTATTAATAGTTGCACTAAT+AGG - chr2_1:69689607-69689626 None:intergenic 25.0%
!!! ATTTGTCATTGTTTTACAAG+TGG + chr2_1:69689948-69689967 Msa0221090:intron 25.0%
!!! CTATCTAGTTTTGTATAACA+TGG + chr2_1:69690018-69690037 Msa0221090:intron 25.0%
! TTGATAAACAAGTTCTTCAT+CGG - chr2_1:69690066-69690085 None:intergenic 25.0%
! GCTGCATTAATACTAAATAT+TGG - chr2_1:69690193-69690212 None:intergenic 25.0%
!!! ATTTCCAAAACAAGTTTTGA+TGG - chr2_1:69690456-69690475 None:intergenic 25.0%
!! TTCAGTATTTTCTTTCATCT+TGG - chr2_1:69690637-69690656 None:intergenic 25.0%
! AAATACTGAAAATTCTTTGG+CGG + chr2_1:69690647-69690666 Msa0221090:CDS 25.0%
TTTCTTAACAGAAACATCCA+AGG - chr2_1:69687432-69687451 None:intergenic 30.0%
CTCTTCAATATCTTTCTTGA+TGG - chr2_1:69687642-69687661 None:intergenic 30.0%
TGAAATTCACAACAAAATGC+AGG + chr2_1:69687709-69687728 Msa0221090:CDS 30.0%
AAAAGTTGAAATGAAGGAAG+AGG + chr2_1:69687733-69687752 Msa0221090:CDS 30.0%
CAGAAAAATTAGAGTCAACA+AGG + chr2_1:69687855-69687874 Msa0221090:CDS 30.0%
TCAAAGCAGAAGCAATTTAA+AGG + chr2_1:69687941-69687960 Msa0221090:CDS 30.0%
ACAATTTGAATCAAAGGAGA+AGG + chr2_1:69688099-69688118 Msa0221090:CDS 30.0%
ACAAAAGAGATACAACATGA+AGG + chr2_1:69688235-69688254 Msa0221090:CDS 30.0%
TCAAAAGAGAAGAATTCCAA+AGG + chr2_1:69688319-69688338 Msa0221090:CDS 30.0%
AGAATTTGAATCAAAGGAGA+AGG + chr2_1:69688477-69688496 Msa0221090:CDS 30.0%
TTAAAAGAGAAGCAACTTGA+AGG + chr2_1:69688571-69688590 Msa0221090:CDS 30.0%
TCAAAGGAGAAGAAATTTGA+AGG + chr2_1:69688613-69688632 Msa0221090:CDS 30.0%
TCAGAAAAGAAGGAATTTGA+AGG + chr2_1:69688823-69688842 Msa0221090:CDS 30.0%
TTAATAGCAAGTCCATCATT+TGG - chr2_1:69688849-69688868 None:intergenic 30.0%
TGAAGAACAAGTTAAATCGA+AGG + chr2_1:69688924-69688943 Msa0221090:CDS 30.0%
AACAGAAACAATTTGAAGGA+CGG + chr2_1:69689007-69689026 Msa0221090:CDS 30.0%
TAAGTTCAAAGTAAAGGTGA+AGG + chr2_1:69689056-69689075 Msa0221090:CDS 30.0%
! TAAATTTGATGGACAACCAA+AGG + chr2_1:69689140-69689159 Msa0221090:CDS 30.0%
ATTGACGAGAAAAAAGAATC+CGG + chr2_1:69689186-69689205 Msa0221090:CDS 30.0%
AGATGAAGAAGAACATTTAC+CGG - chr2_1:69689208-69689227 None:intergenic 30.0%
! TCAGATTTGTTTGAAAGTCT+GGG - chr2_1:69689383-69689402 None:intergenic 30.0%
! TTCAGATTTGTTTGAAAGTC+TGG - chr2_1:69689384-69689403 None:intergenic 30.0%
CAATATTCTGACAAACATTG+TGG + chr2_1:69689568-69689587 Msa0221090:intron 30.0%
ATATTCTGACAAACATTGTG+GGG + chr2_1:69689570-69689589 Msa0221090:intron 30.0%
ATAGCAACTTAACTGAATCA+AGG - chr2_1:69689666-69689685 None:intergenic 30.0%
TGAGTGTTACAGATAATCTA+GGG - chr2_1:69690127-69690146 None:intergenic 30.0%
TTGAGTGTTACAGATAATCT+AGG - chr2_1:69690128-69690147 None:intergenic 30.0%
TATTGGAATGAACTAGATCT+TGG - chr2_1:69690176-69690195 None:intergenic 30.0%
!! AGATCCATCAAAACTTGTTT+TGG + chr2_1:69690449-69690468 Msa0221090:CDS 30.0%
GTTGCTCTAATAGATAGATT+TGG - chr2_1:69690542-69690561 None:intergenic 30.0%
!!! AATGCTTTTTTTCTCACACA+AGG - chr2_1:69690591-69690610 None:intergenic 30.0%
TCTTATACGCCGAAAATTTG+TGG + chr2_1:69687281-69687300 Msa0221090:CDS 35.0%
! TCATCACCACCACAAATTTT+CGG - chr2_1:69687293-69687312 None:intergenic 35.0%
TTATCAAACAGCCTCTTAAC+TGG - chr2_1:69687401-69687420 None:intergenic 35.0%
! TCTTTTCCCTGATCATCATA+CGG - chr2_1:69687467-69687486 None:intergenic 35.0%
TATGATGATCAGGGAAAAGA+TGG + chr2_1:69687467-69687486 Msa0221090:CDS 35.0%
GCTGATGAAAGAACTTCAAT+TGG + chr2_1:69687556-69687575 Msa0221090:CDS 35.0%
! AGTCGTTTAAGAAGTGCAAA+AGG + chr2_1:69687585-69687604 Msa0221090:CDS 35.0%
! TTAAGAAGTGCAAAAGGAAG+AGG + chr2_1:69687591-69687610 Msa0221090:CDS 35.0%
GCAGGAAAAAGTTGAAATGA+AGG + chr2_1:69687727-69687746 Msa0221090:CDS 35.0%
! TTTGGAACTTAAGACCAAAG+AGG + chr2_1:69687793-69687812 Msa0221090:CDS 35.0%
GAATAGCTGTGCTTTAATGA+AGG + chr2_1:69687910-69687929 Msa0221090:CDS 35.0%
AGCAGAAGCAATTTAAAGGT+CGG + chr2_1:69687945-69687964 Msa0221090:CDS 35.0%
CAAACATTTCAAAAATCGGG+TGG + chr2_1:69688159-69688178 Msa0221090:CDS 35.0%
AAGAGATACAACATGAAGGT+CGG + chr2_1:69688239-69688258 Msa0221090:CDS 35.0%
GCATTTCAAAAACCGAATGA+AGG + chr2_1:69688288-69688307 Msa0221090:CDS 35.0%
TTAACTGAAGCTCCTTCATT+TGG - chr2_1:69688555-69688574 None:intergenic 35.0%
AGGAGCTCAAATCAAAATTG+AGG + chr2_1:69688686-69688705 Msa0221090:CDS 35.0%
TCAAAATTGAGGCCATTGAA+AGG + chr2_1:69688697-69688716 Msa0221090:CDS 35.0%
TCTTGAAGTTGTCCTTTCAA+TGG - chr2_1:69688712-69688731 None:intergenic 35.0%
! ACACTTTGAAAGACAAGTGA+AGG + chr2_1:69688792-69688811 Msa0221090:CDS 35.0%
GGAGTTTGAATCAGAAAAGA+AGG + chr2_1:69688813-69688832 Msa0221090:CDS 35.0%
GCATGTTGAAAACCAAATGA+AGG + chr2_1:69688876-69688895 Msa0221090:CDS 35.0%
CCATGGATAAAAAACACATG+AGG - chr2_1:69689453-69689472 None:intergenic 35.0%
!!! CCTCATGTGTTTTTTATCCA+TGG + chr2_1:69689450-69689469 Msa0221090:intron 35.0%
!!! GTGTTTTTTATCCATGGCTA+TGG + chr2_1:69689456-69689475 Msa0221090:intron 35.0%
ATATTGCACAACCACGTAAA+AGG + chr2_1:69689916-69689935 Msa0221090:intron 35.0%
TATTGCACAACCACGTAAAA+GGG + chr2_1:69689917-69689936 Msa0221090:intron 35.0%
! ATTTTTCACACCCTTTTACG+TGG - chr2_1:69689930-69689949 None:intergenic 35.0%
!! ATCCTTTTTCACTGCAATCT+AGG - chr2_1:69689985-69690004 None:intergenic 35.0%
!!! CTAGTTTTGTATAACATGGC+AGG + chr2_1:69690022-69690041 Msa0221090:intron 35.0%
AGATAATCTAGGGAAAGTCA+TGG - chr2_1:69690117-69690136 None:intergenic 35.0%
CATAGGACATTGATGAGTTA+CGG - chr2_1:69690215-69690234 None:intergenic 35.0%
CATGCAAGAATGTAAGACAT+AGG - chr2_1:69690232-69690251 None:intergenic 35.0%
GGAGTTCAATCTCTTTACAA+TGG + chr2_1:69690399-69690418 Msa0221090:CDS 35.0%
! TTTTGACAAAGTGGAGTGAT+GGG - chr2_1:69690489-69690508 None:intergenic 35.0%
ATCACTCCACTTTGTCAAAA+GGG + chr2_1:69690489-69690508 Msa0221090:CDS 35.0%
! TTATCTCCCTTTTGACAAAG+TGG - chr2_1:69690498-69690517 None:intergenic 35.0%
!! GAAGCTAGCATTTGATTTGA+AGG + chr2_1:69690611-69690630 Msa0221090:CDS 35.0%
!! AGATGAAGTTTTCGAGCTTT+TGG + chr2_1:69690725-69690744 Msa0221090:CDS 35.0%
TATACGCCGAAAATTTGTGG+TGG + chr2_1:69687284-69687303 Msa0221090:CDS 40.0%
TCAAAGTCTTGACAAGAAGC+AGG - chr2_1:69687332-69687351 None:intergenic 40.0%
! GTTTGATAACAGCAGTTCCT+TGG + chr2_1:69687412-69687431 Msa0221090:CDS 40.0%
! TTTTCCCTGATCATCATACG+GGG - chr2_1:69687465-69687484 None:intergenic 40.0%
! CTTTTCCCTGATCATCATAC+GGG - chr2_1:69687466-69687485 None:intergenic 40.0%
!! GATTCATCAGCTGATTTGCT+CGG - chr2_1:69687527-69687546 None:intergenic 40.0%
TTGAAGTTCTTTCATCAGCG+AGG - chr2_1:69687555-69687574 None:intergenic 40.0%
CATTAACACAGCTAACTAGC+TGG - chr2_1:69687691-69687710 None:intergenic 40.0%
AAATGAAGGAAGAGGAACTC+AGG + chr2_1:69687741-69687760 Msa0221090:CDS 40.0%
AATGAAGGAAGAGGAACTCA+GGG + chr2_1:69687742-69687761 Msa0221090:CDS 40.0%
TGCATCAAGATCTTCCTCTT+TGG - chr2_1:69687810-69687829 None:intergenic 40.0%
AGAGTCAACAAGGAAGAAGT+TGG + chr2_1:69687865-69687884 Msa0221090:CDS 40.0%
GCAGAAGCAATTTAAAGGTC+GGG + chr2_1:69687946-69687965 Msa0221090:CDS 40.0%
GCAATTTAAAGGTCGGGTTA+AGG + chr2_1:69687952-69687971 Msa0221090:CDS 40.0%
TCAAAAGAGAAGCAGTGTGA+AGG + chr2_1:69687983-69688002 Msa0221090:CDS 40.0%
TCAAAGGAGAAGGAACTTGA+AGG + chr2_1:69688109-69688128 Msa0221090:CDS 40.0%
ACATTTCAAAAATCGGGTGG+AGG + chr2_1:69688162-69688181 Msa0221090:CDS 40.0%
AGAGATACAACATGAAGGTC+GGG + chr2_1:69688240-69688259 Msa0221090:CDS 40.0%
TTGACTCAAGCTCCTTCATT+CGG - chr2_1:69688303-69688322 None:intergenic 40.0%
AGAGAAGAATTCCAAAGGTC+AGG + chr2_1:69688324-69688343 Msa0221090:CDS 40.0%
ACGCATTGAAAGGCAAATGA+AGG + chr2_1:69688372-69688391 Msa0221090:CDS 40.0%
!! GTCAAAAGAGAAGCACTTTG+AGG + chr2_1:69688402-69688421 Msa0221090:CDS 40.0%
!! AAGAGAAGCACTTTGAGGAA+CGG + chr2_1:69688407-69688426 Msa0221090:CDS 40.0%
!! TCAAAGGAGAAGGTACTTGA+AGG + chr2_1:69688487-69688506 Msa0221090:CDS 40.0%
!! GCATTTTGAGTGCCAAATGA+AGG + chr2_1:69688540-69688559 Msa0221090:CDS 40.0%
TTGACTCATGCTCCATCAAT+CGG - chr2_1:69688597-69688616 None:intergenic 40.0%
ATTGATGGAGCATGAGTCAA+AGG + chr2_1:69688597-69688616 Msa0221090:CDS 40.0%
GAAATTTGAAGGCCGTATGA+AGG + chr2_1:69688624-69688643 Msa0221090:CDS 40.0%
CAGATTCCATCTCCTTCATA+CGG - chr2_1:69688639-69688658 None:intergenic 40.0%
! AGAGAAGCATGTTAAAAGCG+TGG + chr2_1:69688660-69688679 Msa0221090:CDS 40.0%
GGAATTTGAAGGCCAAATGA+TGG + chr2_1:69688834-69688853 Msa0221090:CDS 40.0%
TTGACTCAAGCTCCTTCATT+TGG - chr2_1:69688303-69688322 None:intergenic 40.0%
GAAGGAGCTTGAGTCAAAAA+TGG + chr2_1:69688894-69688913 Msa0221090:CDS 40.0%
!! GCATTTTGAAAGACAGGTTG+AGG + chr2_1:69688972-69688991 Msa0221090:CDS 40.0%
AGCCTAGATTGCAGTGAAAA+AGG + chr2_1:69689980-69689999 Msa0221090:intron 40.0%
! TTTTGTATAACATGGCAGGC+TGG + chr2_1:69690026-69690045 Msa0221090:intron 40.0%
ATCTTGGCGAGAAAAGATGT+TGG - chr2_1:69690160-69690179 None:intergenic 40.0%
! TTGCAAACTTGTTCCACCAA+AGG - chr2_1:69690355-69690374 None:intergenic 40.0%
AACACAAGCGAGAAAACTGA+CGG + chr2_1:69690378-69690397 Msa0221090:CDS 40.0%
TTTGACAAAGTGGAGTGATG+GGG - chr2_1:69690488-69690507 None:intergenic 40.0%
! CTTTTGACAAAGTGGAGTGA+TGG - chr2_1:69690490-69690509 None:intergenic 40.0%
CATCACTCCACTTTGTCAAA+AGG + chr2_1:69690488-69690507 Msa0221090:CDS 40.0%
CTCACACAAGGTTCAATAAC+TGG - chr2_1:69690579-69690598 None:intergenic 40.0%
GAAAATTCTTTGGCGGTTCT+TGG + chr2_1:69690654-69690673 Msa0221090:CDS 40.0%
AAACTCTGTTTGCAAAACCC+AGG - chr2_1:69690794-69690813 None:intergenic 40.0%
! GGTTTTGCAAACAGAGTTTC+TGG + chr2_1:69690795-69690814 Msa0221090:CDS 40.0%
! TGGTGATGACGATGTGAAGA+AGG + chr2_1:69687304-69687323 Msa0221090:CDS 45.0%
GATCAGGGAAAAGATGGAGA+TGG + chr2_1:69687473-69687492 Msa0221090:CDS 45.0%
AGGAAGAGGCAAGTGGAAAA+AGG + chr2_1:69687605-69687624 Msa0221090:CDS 45.0%
GGAAGAGGCAAGTGGAAAAA+GGG + chr2_1:69687606-69687625 Msa0221090:CDS 45.0%
GTCAAAGGAGAAGGACTTTG+AGG + chr2_1:69688024-69688043 Msa0221090:CDS 45.0%
! AGGAGAAGGACTTTGAGGAA+AGG + chr2_1:69688029-69688048 Msa0221090:CDS 45.0%
! GGAGAAGGACTTTGAGGAAA+GGG + chr2_1:69688030-69688049 Msa0221090:CDS 45.0%
AGGAGCTTGATGCAAAAGAG+AGG + chr2_1:69688182-69688201 Msa0221090:CDS 45.0%
! GCAAAAGAGAGGCAAGTTGA+AGG + chr2_1:69688193-69688212 Msa0221090:CDS 45.0%
GAATTCCAAAGGTCAGGTGA+AGG + chr2_1:69688330-69688349 Msa0221090:CDS 45.0%
!! AGAGAAGCACTTTGAGGAAC+GGG + chr2_1:69688408-69688427 Msa0221090:CDS 45.0%
! GAAGCATGTTAAAAGCGTGG+TGG + chr2_1:69688663-69688682 Msa0221090:CDS 45.0%
GCAACTTGATGTCCAAGTGA+AGG + chr2_1:69688750-69688769 Msa0221090:CDS 45.0%
TAGATTCCAGCTCCTTCACT+TGG - chr2_1:69688765-69688784 None:intergenic 45.0%
TGAAGGACGGTTGAATGAAC+TGG + chr2_1:69689020-69689039 Msa0221090:CDS 45.0%
ACGGTTGAATGAACTGGAGT+CGG + chr2_1:69689026-69689045 Msa0221090:CDS 45.0%
GCAGTTTGAAGGACAAGTCA+AGG + chr2_1:69689098-69689117 Msa0221090:CDS 45.0%
!! CATGGAACAAGCTCTGAAGT+TGG - chr2_1:69690099-69690118 None:intergenic 45.0%
GATCAATCAAGTCGTGCCTT+TGG + chr2_1:69690336-69690355 Msa0221090:CDS 45.0%
AAAATTGCCGTCGAGCTGTT+TGG + chr2_1:69690765-69690784 Msa0221090:CDS 45.0%
CTGATGCACTGCCAGTTAAG+AGG + chr2_1:69687387-69687406 Msa0221090:CDS 50.0%
GTGCAAAAGGAAGAGGCAAG+TGG + chr2_1:69687598-69687617 Msa0221090:CDS 50.0%
AGTGGAGGAGCTTCAGTCAA+AGG + chr2_1:69688009-69688028 Msa0221090:CDS 50.0%
GGAGCTTCAGTCAAAGGAGA+AGG + chr2_1:69688015-69688034 Msa0221090:CDS 50.0%
! GCAAGTTGAAGGACGAGCAA+TGG + chr2_1:69688204-69688223 Msa0221090:CDS 50.0%
ACAACATGAAGGTCGGGTGA+AGG + chr2_1:69688246-69688265 Msa0221090:CDS 50.0%
CAACTCCTTCACCTGACCTT+TGG - chr2_1:69688338-69688357 None:intergenic 50.0%
CAAAGGTCAGGTGAAGGAGT+TGG + chr2_1:69688336-69688355 Msa0221090:CDS 50.0%
TGAGGAACGGGTAAAGAAGC+TGG + chr2_1:69688420-69688439 Msa0221090:CDS 50.0%
AGAAGCTGGAGTCAAGAGAG+AGG + chr2_1:69688434-69688453 Msa0221090:CDS 50.0%
GCAACTTGAAGGCCGATTGA+TGG + chr2_1:69688582-69688601 Msa0221090:CDS 50.0%
TGAAGGCCGTATGAAGGAGA+TGG + chr2_1:69688630-69688649 Msa0221090:CDS 50.0%
! GCATGTTAAAAGCGTGGTGG+AGG + chr2_1:69688666-69688685 Msa0221090:CDS 50.0%
TGATGTCCAAGTGAAGGAGC+TGG + chr2_1:69688756-69688775 Msa0221090:CDS 50.0%
AACCAAAGGAGCCTGAGTCA+AGG + chr2_1:69689154-69689173 Msa0221090:CDS 50.0%
TTCCTTGACTCAGGCTCCTT+TGG - chr2_1:69689159-69689178 None:intergenic 50.0%
!! CAAGCTCTGAAGTTGGATGC+TGG - chr2_1:69690092-69690111 None:intergenic 50.0%
CAATCAAGTCGTGCCTTTGG+TGG + chr2_1:69690339-69690358 Msa0221090:CDS 50.0%
GCGTCCCCGTATGATGATCA+GGG + chr2_1:69687458-69687477 Msa0221090:CDS 55.0%
GAAGCAGTGTGAAGGACGAG+TGG + chr2_1:69687991-69688010 Msa0221090:CDS 55.0%
CAGGCTTCCAAACAGCTCGA+CGG - chr2_1:69690775-69690794 None:intergenic 55.0%
GTCGAGCTGTTTGGAAGCCT+GGG + chr2_1:69690774-69690793 Msa0221090:CDS 55.0%
GGCGTCCCCGTATGATGATC+AGG + chr2_1:69687457-69687476 Msa0221090:CDS 60.0%
GCAGTGTGAAGGACGAGTGG+AGG + chr2_1:69687994-69688013 Msa0221090:CDS 60.0%
CGTCGAGCTGTTTGGAAGCC+TGG + chr2_1:69690773-69690792 Msa0221090:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 69687263 69690830 69687263 ID=Msa0221090;Name=Msa0221090
chr2_1 mRNA 69687263 69690830 69687263 ID=Msa0221090-mRNA-1;Parent=Msa0221090;Name=Msa0221090-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|1|0|1|1|1|2|0|818
chr2_1 exon 69687263 69689207 69687263 ID=Msa0221090-mRNA-1:exon:19749;Parent=Msa0221090-mRNA-1
chr2_1 exon 69690319 69690830 69690319 ID=Msa0221090-mRNA-1:exon:19750;Parent=Msa0221090-mRNA-1
chr2_1 CDS 69687263 69689207 69687263 ID=Msa0221090-mRNA-1:cds;Parent=Msa0221090-mRNA-1
chr2_1 CDS 69690319 69690830 69690319 ID=Msa0221090-mRNA-1:cds;Parent=Msa0221090-mRNA-1
Gene Sequence

>Msa0221090

ATGATGGATGTTGAGGCTTCTTATACGCCGAAAATTTGTGGTGGTGATGACGATGTGAAGAAGGAACCTGCTTCTTGTCAAGACTTTGATTCAAGTTTGAGAACATGTAGTAGTAAATGTGATACTGATGCACTGCCAGTTAAGAGGCTGTTTGATAACAGCAGTTCCTTGGATGTTTCTGTTAAGAAATCAAAGGCGTCCCCGTATGATGATCAGGGAAAAGATGGAGATGGTATTCTGCTGACAAGTACAACTAAAAAGCCGAGCAAATCAGCTGATGAATCATTTTCCTCGCTGATGAAAGAACTTCAATTGGTAGAAAAGTCGTTTAAGAAGTGCAAAAGGAAGAGGCAAGTGGAAAAAGGGAGATTGCAGTCCATCAAGAAAGATATTGAAGAGTGCAGCAAAGAGCTTGAAGATAAGAACCAGCTAGTTAGCTGTGTTAATGAAATTCACAACAAAATGCAGGAAAAAGTTGAAATGAAGGAAGAGGAACTCAGGGCGCTTTCCCAAAAAGTTACTGAATGTATTTTGGAACTTAAGACCAAAGAGGAAGATCTTGATGCAGTGAACAAATTAATTAGTGAAGAAACAGAAAAATTAGAGTCAACAAGGAAGAAGTTGGTAAATATAAGATCAGAAATGAAGAATAGCTGTGCTTTAATGAAGGAATTTGAGTCAAAGCAGAAGCAATTTAAAGGTCGGGTTAAGGAACTCGAGTCAAAAGAGAAGCAGTGTGAAGGACGAGTGGAGGAGCTTCAGTCAAAGGAGAAGGACTTTGAGGAAAGGGTAAAGAAGCTAGAGTCAAGAGAGAAGCAGCTTGAAGTCCATGTGAAACAATTTGAATCAAAGGAGAAGGAACTTGAAGGTTATATGAATGAGCTTGAATCAGAAAACAAACATTTCAAAAATCGGGTGGAGGAGCTTGATGCAAAAGAGAGGCAAGTTGAAGGACGAGCAATGGAGCTAGAGACAAAAGAGATACAACATGAAGGTCGGGTGAAGGAGTTTGAATCAAAAGAGAAGCATTTCAAAAACCGAATGAAGGAGCTTGAGTCAAAAGAGAAGAATTCCAAAGGTCAGGTGAAGGAGTTGGAATTTAAAAAGAAACGCATTGAAAGGCAAATGAAGGAGCTTGAGTCAAAAGAGAAGCACTTTGAGGAACGGGTAAAGAAGCTGGAGTCAAGAGAGAGGCAGTTTGAAGTCCGTGTGAAAGAATTTGAATCAAAGGAGAAGGTACTTGAAGGTCATACGAATGAGCTTGAATCAAAAAAGAAGCATTTTGAGTGCCAAATGAAGGAGCTTCAGTTAAAAGAGAAGCAACTTGAAGGCCGATTGATGGAGCATGAGTCAAAGGAGAAGAAATTTGAAGGCCGTATGAAGGAGATGGAATCTGAAGAGAAGCATGTTAAAAGCGTGGTGGAGGAGCTCAAATCAAAATTGAGGCCATTGAAAGGACAACTTCAAGAACTTTACTCTAAAGAGAAGCAACTTGATGTCCAAGTGAAGGAGCTGGAATCTAAAAACAGACACTTTGAAAGACAAGTGAAGGAGTTTGAATCAGAAAAGAAGGAATTTGAAGGCCAAATGATGGACTTGCTATTAAAAGAGAAGCATGTTGAAAACCAAATGAAGGAGCTTGAGTCAAAAATGGAGAAATTTGAAGAACAAGTTAAATCGAAGGAGAAAGAACTCGAATCTAAAAATAAGCATTTTGAAAGACAGGTTGAGGACTTCAAATCAAAACAGAAACAATTTGAAGGACGGTTGAATGAACTGGAGTCGGAAGAGTATAAGTTCAAAGTAAAGGTGAAGGAGCTCAACTTAAAAGAAAAGCAGTTTGAAGGACAAGTCAAGGATCCTAAGTCAAAGCTGAATAAATTTGATGGACAACCAAAGGAGCCTGAGTCAAGGAAAAAATATATTGACGAGAAAAAAGAATCCGTTGCATCTTACATGCATGATCAATCAAGTCGTGCCTTTGGTGGAACAAGTTTGCAATTGAACACAAGCGAGAAAACTGACGGAGTTCAATCTCTTTACAATGGTATTTTAGTTAATCTGCAAGACTCATCAGATCCATCAAAACTTGTTTTGGAAATGATACTGAACCCCATCACTCCACTTTGTCAAAAGGGAGATAATGTTGTGATTATTGCTGATTACCAAATCTATCTATTAGAGCAACTGATGAGAATCTCACCAGTTATTGAACCTTGTGTGAGAAAAAAAGCATTGAAGCTAGCATTTGATTTGAAGGCCAAGATGAAAGAAAATACTGAAAATTCTTTGGCGGTTCTTGGTTTTCTACAGCTTTTGTCAATTTATAAATTGCTTGATTCGTTCGATAAAGATGAAGTTTTCGAGCTTTTGGCATTTGTTGCTCTGCACAAAATTGCCGTCGAGCTGTTTGGAAGCCTGGGTTTTGCAAACAGAGTTTCTGGTATGTTGATTTAG
Protein sequence

>Msa0221090

MMDVEASYTPKICGGDDDVKKEPASCQDFDSSLRTCSSKCDTDALPVKRLFDNSSSLDVSVKKSKASPYDDQGKDGDGILLTSTTKKPSKSADESFSSLMKELQLVEKSFKKCKRKRQVEKGRLQSIKKDIEECSKELEDKNQLVSCVNEIHNKMQEKVEMKEEELRALSQKVTECILELKTKEEDLDAVNKLISEETEKLESTRKKLVNIRSEMKNSCALMKEFESKQKQFKGRVKELESKEKQCEGRVEELQSKEKDFEERVKKLESREKQLEVHVKQFESKEKELEGYMNELESENKHFKNRVEELDAKERQVEGRAMELETKEIQHEGRVKEFESKEKHFKNRMKELESKEKNSKGQVKELEFKKKRIERQMKELESKEKHFEERVKKLESRERQFEVRVKEFESKEKVLEGHTNELESKKKHFECQMKELQLKEKQLEGRLMEHESKEKKFEGRMKEMESEEKHVKSVVEELKSKLRPLKGQLQELYSKEKQLDVQVKELESKNRHFERQVKEFESEKKEFEGQMMDLLLKEKHVENQMKELESKMEKFEEQVKSKEKELESKNKHFERQVEDFKSKQKQFEGRLNELESEEYKFKVKVKELNLKEKQFEGQVKDPKSKLNKFDGQPKEPESRKKYIDEKKESVASYMHDQSSRAFGGTSLQLNTSEKTDGVQSLYNGILVNLQDSSDPSKLVLEMILNPITPLCQKGDNVVIIADYQIYLLEQLMRISPVIEPCVRKKALKLAFDLKAKMKENTENSLAVLGFLQLLSIYKLLDSFDKDEVFELLAFVALHKIAVELFGSLGFANRVSGMLI*