Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1437950 | AES61567.1 | 96.774 | 93 | 3 | 0 | 1 | 93 | 1 | 93 | 3.36e-57 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1437950 | sp|Q6NNN0|RADL3_ARATH | 55.738 | 61 | 26 | 1 | 11 | 71 | 12 | 71 | 6.50e-17 | 71.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1437950 | G7IBR6 | 96.774 | 93 | 3 | 0 | 1 | 93 | 1 | 93 | 1.60e-57 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0022310 | Msa1437950 | 0.806899 | 6.573313e-50 | -8.615850e-47 |
| Msa0067360 | Msa1437950 | 0.824102 | 9.680181e-54 | -8.615850e-47 |
| Msa1369530 | Msa1437950 | 0.809312 | 2.014075e-50 | -8.615850e-47 |
| Msa0621590 | Msa1437950 | 0.807842 | 4.149476e-50 | -8.615850e-47 |
| Msa0691050 | Msa1437950 | 0.804382 | 2.219270e-49 | -8.615850e-47 |
| Msa0220740 | Msa1437950 | 0.806856 | 6.714268e-50 | -8.615850e-47 |
| Msa0222920 | Msa1437950 | 0.825884 | 3.673688e-54 | -8.615850e-47 |
| Msa0990960 | Msa1437950 | 0.830626 | 2.641758e-55 | -8.615850e-47 |
| Msa1040120 | Msa1437950 | 0.805707 | 1.172293e-49 | -8.615850e-47 |
| Msa1046300 | Msa1437950 | 0.806873 | 6.659197e-50 | -8.615850e-47 |
| Msa0344830 | Msa1437950 | 0.831447 | 1.660413e-55 | -8.615850e-47 |
| Msa0891040 | Msa1437950 | 0.812966 | 3.249252e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1437950 | MtrunA17_Chr1g0194071 | 96.774 | 93 | 3 | 0 | 1 | 93 | 1 | 93 | 3.09e-61 | 181 |
| Msa1437950 | MtrunA17_Chr7g0270711 | 71.739 | 92 | 21 | 1 | 1 | 92 | 1 | 87 | 2.73e-40 | 128 |
| Msa1437950 | MtrunA17_Chr3g0144631 | 59.649 | 57 | 22 | 1 | 11 | 67 | 16 | 71 | 4.78e-16 | 66.6 |
| Msa1437950 | MtrunA17_Chr6g0449111 | 48.333 | 60 | 30 | 1 | 9 | 68 | 8 | 66 | 6.37e-15 | 63.5 |
| Msa1437950 | MtrunA17_Chr3g0081631 | 51.724 | 58 | 28 | 0 | 11 | 68 | 10 | 67 | 7.30e-15 | 63.9 |
| Msa1437950 | MtrunA17_Chr8g0373131 | 52.542 | 59 | 26 | 1 | 9 | 67 | 3 | 59 | 2.39e-14 | 62.0 |
| Msa1437950 | MtrunA17_Chr8g0373111 | 52.542 | 59 | 26 | 1 | 9 | 67 | 3 | 59 | 2.49e-14 | 62.0 |
| Msa1437950 | MtrunA17_Chr8g0373121 | 52.542 | 59 | 26 | 1 | 9 | 67 | 3 | 59 | 2.49e-14 | 62.0 |
| Msa1437950 | MtrunA17_Chr1g0155181 | 49.153 | 59 | 29 | 1 | 9 | 67 | 5 | 62 | 3.16e-14 | 62.0 |
| Msa1437950 | MtrunA17_Chr5g0404301 | 48.333 | 60 | 29 | 1 | 8 | 67 | 1 | 58 | 1.22e-13 | 60.1 |
| Msa1437950 | MtrunA17_Chr7g0255841 | 56.364 | 55 | 23 | 1 | 14 | 68 | 15 | 68 | 1.25e-13 | 60.8 |
| Msa1437950 | MtrunA17_Chr8g0373101 | 48.276 | 58 | 28 | 1 | 11 | 68 | 5 | 60 | 3.26e-13 | 59.3 |
| Msa1437950 | MtrunA17_Chr5g0404101 | 50.000 | 58 | 28 | 1 | 11 | 68 | 19 | 75 | 5.65e-13 | 59.3 |
| Msa1437950 | MtrunA17_Chr3g0141601 | 47.458 | 59 | 30 | 1 | 9 | 67 | 5 | 62 | 3.20e-12 | 57.0 |
| Msa1437950 | MtrunA17_Chr3g0141641 | 53.571 | 56 | 25 | 1 | 10 | 65 | 2 | 56 | 4.72e-11 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1437950 | AT1G75250.2 | 41.975 | 81 | 43 | 2 | 7 | 84 | 8 | 87 | 5.50e-16 | 67.4 |
| Msa1437950 | AT1G75250.1 | 41.975 | 81 | 43 | 2 | 7 | 84 | 8 | 87 | 1.16e-15 | 67.0 |
| Msa1437950 | AT4G39250.1 | 49.123 | 57 | 28 | 1 | 11 | 67 | 14 | 69 | 5.69e-14 | 62.0 |
| Msa1437950 | AT2G18328.1 | 50.000 | 56 | 27 | 1 | 14 | 69 | 14 | 68 | 9.39e-14 | 60.8 |
| Msa1437950 | AT1G19510.1 | 56.604 | 53 | 22 | 1 | 14 | 66 | 15 | 66 | 9.96e-13 | 58.9 |
| Msa1437950 | AT2G21650.1 | 47.368 | 57 | 29 | 1 | 11 | 67 | 14 | 69 | 1.98e-12 | 58.2 |
| Msa1437950 | AT5G04760.1 | 48.333 | 60 | 29 | 1 | 11 | 70 | 6 | 63 | 6.68e-12 | 59.3 |
| Msa1437950 | AT4G36570.1 | 61.364 | 44 | 16 | 1 | 11 | 54 | 12 | 54 | 2.71e-11 | 54.3 |
Find 21 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAAAATTTGATCATAAAT+TGG | 0.124686 | tig0028402:-39214 | Msa1437950:CDS |
| GCAACCATCTCCCACCTTTC+TGG | 0.164171 | tig0028402:+39321 | None:intergenic |
| AGAGCATTATGTGATACTTT+TGG | 0.282735 | tig0028402:-39259 | Msa1437950:CDS |
| GATATTCTTAAGGGGTTATG+TGG | 0.360269 | tig0028402:-39405 | Msa1437950:CDS |
| GGAAAATTTGATCATAAATT+GGG | 0.369552 | tig0028402:-39213 | Msa1437950:CDS |
| AGGGGTTATGTGGGTGGAGT+TGG | 0.375226 | tig0028402:-39395 | Msa1437950:CDS |
| TGACATGGATGATATTCTTA+AGG | 0.387299 | tig0028402:-39415 | None:intergenic |
| GATTTGGAGCTTATAGAATC+TGG | 0.404881 | tig0028402:-39234 | Msa1437950:CDS |
| GACATGGATGATATTCTTAA+GGG | 0.427645 | tig0028402:-39414 | Msa1437950:CDS |
| TTCTTAAGGGGTTATGTGGG+TGG | 0.506774 | tig0028402:-39401 | Msa1437950:CDS |
| GAGATGGTTGCAGCTATGGT+TGG | 0.516331 | tig0028402:-39309 | Msa1437950:CDS |
| GTGGGAGATGGTTGCAGCTA+TGG | 0.520778 | tig0028402:-39313 | Msa1437950:CDS |
| ATATTCTTAAGGGGTTATGT+GGG | 0.528932 | tig0028402:-39404 | Msa1437950:CDS |
| TCATCCAGAAAGGTGGGAGA+TGG | 0.560419 | tig0028402:-39325 | Msa1437950:CDS |
| ACAAGCATAAGGACTCAGTA+AGG | 0.572830 | tig0028402:+39163 | None:intergenic |
| ATGAGAGTCATCCAGAAAGG+TGG | 0.588731 | tig0028402:-39332 | Msa1437950:CDS |
| GTTATGTGGGTGGAGTTGGA+AGG | 0.593267 | tig0028402:-39391 | Msa1437950:CDS |
| TGAGAGTCATCCAGAAAGGT+GGG | 0.602388 | tig0028402:-39331 | Msa1437950:CDS |
| ATGGTTGCAGCTATGGTTGG+AGG | 0.608350 | tig0028402:-39306 | Msa1437950:CDS |
| TTGATGAGAGTCATCCAGAA+AGG | 0.613257 | tig0028402:-39335 | Msa1437950:CDS |
| ACATGGATGATATTCTTAAG+GGG | 0.632105 | tig0028402:-39413 | Msa1437950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGGAAAATTTGATCATAAAT+TGG | - | tig0028402:39349-39368 | Msa1437950:CDS | 20.0% |
| !! | GGAAAATTTGATCATAAATT+GGG | - | tig0028402:39350-39369 | Msa1437950:CDS | 20.0% |
| !!! | TTATTTTTCAGACAAGCATA+AGG | + | tig0028402:39414-39433 | None:intergenic | 25.0% |
| ATATTCTTAAGGGGTTATGT+GGG | - | tig0028402:39159-39178 | Msa1437950:CDS | 30.0% | |
| !! | AGAGCATTATGTGATACTTT+TGG | - | tig0028402:39304-39323 | Msa1437950:CDS | 30.0% |
| GATATTCTTAAGGGGTTATG+TGG | - | tig0028402:39158-39177 | Msa1437950:CDS | 35.0% | |
| ! | GCATTATGTGATACTTTTGG+AGG | - | tig0028402:39307-39326 | Msa1437950:CDS | 35.0% |
| !! | TGTGATACTTTTGGAGGATT+TGG | - | tig0028402:39313-39332 | Msa1437950:CDS | 35.0% |
| ! | GATTTGGAGCTTATAGAATC+TGG | - | tig0028402:39329-39348 | Msa1437950:CDS | 35.0% |
| TTGATGAGAGTCATCCAGAA+AGG | - | tig0028402:39228-39247 | Msa1437950:CDS | 40.0% | |
| ! | TTGGAGGTGAAAAAAGTGCA+GGG | - | tig0028402:39273-39292 | Msa1437950:CDS | 40.0% |
| ACAAGCATAAGGACTCAGTA+AGG | + | tig0028402:39403-39422 | None:intergenic | 40.0% | |
| TTCTTAAGGGGTTATGTGGG+TGG | - | tig0028402:39162-39181 | Msa1437950:CDS | 45.0% | |
| ATGAGAGTCATCCAGAAAGG+TGG | - | tig0028402:39231-39250 | Msa1437950:CDS | 45.0% | |
| TGAGAGTCATCCAGAAAGGT+GGG | - | tig0028402:39232-39251 | Msa1437950:CDS | 45.0% | |
| ! | GTTGGAGGTGAAAAAAGTGC+AGG | - | tig0028402:39272-39291 | Msa1437950:CDS | 45.0% |
| ! | TGGAGGTGAAAAAAGTGCAG+GGG | - | tig0028402:39274-39293 | Msa1437950:CDS | 45.0% |
| GTTATGTGGGTGGAGTTGGA+AGG | - | tig0028402:39172-39191 | Msa1437950:CDS | 50.0% | |
| TCATCCAGAAAGGTGGGAGA+TGG | - | tig0028402:39238-39257 | Msa1437950:CDS | 50.0% | |
| GAGATGGTTGCAGCTATGGT+TGG | - | tig0028402:39254-39273 | Msa1437950:CDS | 50.0% | |
| ATGGTTGCAGCTATGGTTGG+AGG | - | tig0028402:39257-39276 | Msa1437950:CDS | 50.0% | |
| ! | AGGTGAAAAAAGTGCAGGGG+AGG | - | tig0028402:39277-39296 | Msa1437950:CDS | 50.0% |
| AGGGGTTATGTGGGTGGAGT+TGG | - | tig0028402:39168-39187 | Msa1437950:CDS | 55.0% | |
| GCAACCATCTCCCACCTTTC+TGG | + | tig0028402:39245-39264 | None:intergenic | 55.0% | |
| GTGGGAGATGGTTGCAGCTA+TGG | - | tig0028402:39250-39269 | Msa1437950:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0028402 | gene | 39152 | 39433 | 39152 | ID=Msa1437950;Name=Msa1437950 |
| tig0028402 | mRNA | 39152 | 39433 | 39152 | ID=Msa1437950-mRNA-1;Parent=Msa1437950;Name=Msa1437950-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|93 |
| tig0028402 | exon | 39152 | 39433 | 39152 | ID=Msa1437950-mRNA-1:exon:11050;Parent=Msa1437950-mRNA-1 |
| tig0028402 | CDS | 39152 | 39433 | 39152 | ID=Msa1437950-mRNA-1:cds;Parent=Msa1437950-mRNA-1 |
| Gene Sequence |
| Protein sequence |
