Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0022310 | Msa0025330 | 0.813380 | 2.636109e-51 | -8.615850e-47 |
| Msa0022310 | Msa0045790 | 0.809412 | 1.917091e-50 | -8.615850e-47 |
| Msa0022310 | Msa0046160 | 0.806437 | 8.231214e-50 | -8.615850e-47 |
| Msa0022310 | Msa0072900 | 0.815415 | 9.351717e-52 | -8.615850e-47 |
| Msa0022310 | Msa0077450 | 0.816467 | 5.447365e-52 | -8.615850e-47 |
| Msa0022310 | Msa0101810 | -0.815890 | 7.332747e-52 | -8.615850e-47 |
| Msa0022310 | Msa0121090 | 0.807821 | 4.190809e-50 | -8.615850e-47 |
| Msa0022310 | Msa0123100 | 0.814128 | 1.803856e-51 | -8.615850e-47 |
| Msa0022310 | Msa0133980 | -0.802315 | 5.945761e-49 | -8.615850e-47 |
| Msa0022310 | Msa0160010 | 0.805434 | 1.337555e-49 | -8.615850e-47 |
| Msa0022310 | Msa0160130 | 0.819172 | 1.335107e-52 | -8.615850e-47 |
| Msa0022310 | Msa0161530 | 0.847923 | 8.502017e-60 | -8.615850e-47 |
| Msa0022310 | Msa0170140 | 0.829707 | 4.427404e-55 | -8.615850e-47 |
| Msa0022310 | Msa0171300 | 0.805276 | 1.443084e-49 | -8.615850e-47 |
| Msa0022310 | Msa0215320 | -0.804265 | 2.346981e-49 | -8.615850e-47 |
| Msa0022310 | Msa0276920 | 0.808590 | 2.874158e-50 | -8.615850e-47 |
| Msa0022310 | Msa0279790 | 0.805272 | 1.445907e-49 | -8.615850e-47 |
| Msa0022310 | Msa0283450 | 0.805801 | 1.120284e-49 | -8.615850e-47 |
| Msa0022310 | Msa0283520 | 0.804199 | 2.422471e-49 | -8.615850e-47 |
| Msa0022310 | Msa0285450 | 0.802640 | 5.098141e-49 | -8.615850e-47 |
| Msa0022310 | Msa0323080 | 0.818512 | 1.885898e-52 | -8.615850e-47 |
| Msa0022310 | Msa0332000 | 0.805612 | 1.227311e-49 | -8.615850e-47 |
| Msa0022310 | Msa0333030 | 0.801326 | 9.493234e-49 | -8.615850e-47 |
| Msa0022310 | Msa0333720 | -0.809828 | 1.560379e-50 | -8.615850e-47 |
| Msa0022310 | Msa0350990 | 0.829609 | 4.678703e-55 | -8.615850e-47 |
| Msa0022310 | Msa0351030 | 0.833178 | 6.191568e-56 | -8.615850e-47 |
| Msa0022310 | Msa0351080 | 0.819194 | 1.319718e-52 | -8.615850e-47 |
| Msa0022310 | Msa0351100 | 0.808868 | 2.506393e-50 | -8.615850e-47 |
| Msa0022310 | Msa0356890 | -0.804817 | 1.800153e-49 | -8.615850e-47 |
| Msa0022310 | Msa0366240 | 0.812192 | 4.798358e-51 | -8.615850e-47 |
| Msa0022310 | Msa0376590 | 0.802053 | 6.732382e-49 | -8.615850e-47 |
| Msa0022310 | Msa0406880 | 0.808075 | 3.700945e-50 | -8.615850e-47 |
| Msa0022310 | Msa0436370 | 0.812786 | 3.557711e-51 | -8.615850e-47 |
| Msa0022310 | Msa0443260 | 0.801432 | 9.027751e-49 | -8.615850e-47 |
| Msa0022310 | Msa0484780 | 0.804575 | 2.022219e-49 | -8.615850e-47 |
| Msa0022310 | Msa0516370 | 0.808631 | 2.816744e-50 | -8.615850e-47 |
| Msa0022310 | Msa0532350 | 0.805269 | 1.448055e-49 | -8.615850e-47 |
| Msa0022310 | Msa0572420 | 0.815586 | 8.570509e-52 | -8.615850e-47 |
| Msa0022310 | Msa0572480 | 0.817132 | 3.864518e-52 | -8.615850e-47 |
| Msa0022310 | Msa0593650 | 0.808940 | 2.419245e-50 | -8.615850e-47 |
| Msa0022310 | Msa0608200 | 0.846010 | 2.840130e-59 | -8.615850e-47 |
| Msa0022310 | Msa0621590 | 0.800609 | 1.329915e-48 | -8.615850e-47 |
| Msa0022310 | Msa0623080 | 0.821551 | 3.802928e-53 | -8.615850e-47 |
| Msa0022310 | Msa0640860 | 0.821393 | 4.134513e-53 | -8.615850e-47 |
| Msa0022310 | Msa0648520 | 0.838599 | 2.613325e-57 | -8.615850e-47 |
| Msa0022310 | Msa0658570 | 0.800418 | 1.455087e-48 | -8.615850e-47 |
| Msa0022310 | Msa0689010 | 0.813046 | 3.120670e-51 | -8.615850e-47 |
| Msa0022310 | Msa0691050 | 0.877086 | 8.332673e-69 | -8.615850e-47 |
| Msa0022310 | Msa0693380 | 0.814608 | 1.412809e-51 | -8.615850e-47 |
| Msa0022310 | Msa0713640 | 0.801939 | 7.106913e-49 | -8.615850e-47 |
| Msa0022310 | Msa0731940 | 0.820277 | 7.468660e-53 | -8.615850e-47 |
| Msa0022310 | Msa0735170 | 0.821501 | 3.905471e-53 | -8.615850e-47 |
| Msa0022310 | Msa0737570 | 0.807148 | 5.823302e-50 | -8.615850e-47 |
| Msa0022310 | Msa0760130 | 0.802097 | 6.593058e-49 | -8.615850e-47 |
| Msa0022310 | Msa0810220 | 0.803325 | 3.679001e-49 | -8.615850e-47 |
| Msa0022310 | Msa0834880 | 0.807372 | 5.219455e-50 | -8.615850e-47 |
| Msa0022310 | Msa0850960 | 0.813544 | 2.426391e-51 | -8.615850e-47 |
| Msa0022310 | Msa0856920 | 0.812721 | 3.677270e-51 | -8.615850e-47 |
| Msa0022310 | Msa0870530 | 0.806115 | 9.620875e-50 | -8.615850e-47 |
| Msa0022310 | Msa0880950 | 0.803097 | 4.100665e-49 | -8.615850e-47 |
| Msa0022310 | Msa0891040 | 0.834624 | 2.691186e-56 | -8.615850e-47 |
| Msa0022310 | Msa0894860 | 0.807256 | 5.523589e-50 | -8.615850e-47 |
| Msa0022310 | Msa0903700 | 0.817316 | 3.513521e-52 | -8.615850e-47 |
| Msa0022310 | Msa0907070 | 0.810347 | 1.205800e-50 | -8.615850e-47 |
| Msa0022310 | Msa0936210 | 0.804933 | 1.702950e-49 | -8.615850e-47 |
| Msa0022310 | Msa0969520 | 0.804055 | 2.595446e-49 | -8.615850e-47 |
| Msa0022310 | Msa0975220 | 0.813030 | 3.146467e-51 | -8.615850e-47 |
| Msa0022310 | Msa0990960 | 0.830925 | 2.231774e-55 | -8.615850e-47 |
| Msa0022310 | Msa0998690 | 0.806529 | 7.870854e-50 | -8.615850e-47 |
| Msa0022310 | Msa1000300 | 0.805830 | 1.104588e-49 | -8.615850e-47 |
| Msa0022310 | Msa1014540 | 0.805560 | 1.258391e-49 | -8.615850e-47 |
| Msa0022310 | Msa1024740 | 0.807032 | 6.160418e-50 | -8.615850e-47 |
| Msa0022310 | Msa1027630 | 0.803136 | 4.026106e-49 | -8.615850e-47 |
| Msa0022310 | Msa1035300 | 0.808949 | 2.408115e-50 | -8.615850e-47 |
| Msa0022310 | Msa1046300 | 0.821210 | 4.558083e-53 | -8.615850e-47 |
| Msa0022310 | Msa1075220 | -0.803640 | 3.166076e-49 | -8.615850e-47 |
| Msa0022310 | Msa1080960 | 0.804590 | 2.007690e-49 | -8.615850e-47 |
| Msa0022310 | Msa1082500 | 0.810392 | 1.179147e-50 | -8.615850e-47 |
| Msa0022310 | Msa1104370 | 0.801300 | 9.608951e-49 | -8.615850e-47 |
| Msa0022310 | Msa1116450 | 0.804860 | 1.763761e-49 | -8.615850e-47 |
| Msa0022310 | Msa1130550 | 0.807739 | 4.362275e-50 | -8.615850e-47 |
| Msa0022310 | Msa1152210 | 0.802075 | 6.662406e-49 | -8.615850e-47 |
| Msa0022310 | Msa1162550 | -0.806328 | 8.676136e-50 | -8.615850e-47 |
| Msa0022310 | Msa1229600 | 0.806015 | 1.009658e-49 | -8.615850e-47 |
| Msa0022310 | Msa1231550 | 0.826879 | 2.129493e-54 | -8.615850e-47 |
| Msa0022310 | Msa1238930 | 0.804960 | 1.680899e-49 | -8.615850e-47 |
| Msa0022310 | Msa1270610 | 0.800053 | 1.726021e-48 | -8.615850e-47 |
| Msa0022310 | Msa1310210 | 0.824929 | 6.183914e-54 | -8.615850e-47 |
| Msa0022310 | Msa1311960 | 0.802066 | 6.690526e-49 | -8.615850e-47 |
| Msa0022310 | Msa1323670 | 0.810549 | 1.090554e-50 | -8.615850e-47 |
| Msa0022310 | Msa1331590 | 0.811319 | 7.429519e-51 | -8.615850e-47 |
| Msa0022310 | Msa1341520 | 0.814501 | 1.491824e-51 | -8.615850e-47 |
| Msa0022310 | Msa1350310 | 0.815960 | 7.072855e-52 | -8.615850e-47 |
| Msa0022310 | Msa1379850 | 0.812510 | 4.088731e-51 | -8.615850e-47 |
| Msa0022310 | Msa1390120 | 0.810395 | 1.177316e-50 | -8.615850e-47 |
| Msa0022310 | Msa1392260 | 0.800341 | 1.508531e-48 | -8.615850e-47 |
| Msa0022310 | Msa1393410 | 0.809306 | 2.020073e-50 | -8.615850e-47 |
| Msa0022310 | Msa1395530 | 0.814381 | 1.585629e-51 | -8.615850e-47 |
| Msa0022310 | Msa1408620 | -0.809342 | 1.983916e-50 | -8.615850e-47 |
| Msa0022310 | Msa1409700 | 0.801495 | 8.767016e-49 | -8.615850e-47 |
| Msa0022310 | Msa1409870 | 0.804199 | 2.422768e-49 | -8.615850e-47 |
| Msa0022310 | Msa1423880 | 0.814065 | 1.862587e-51 | -8.615850e-47 |
| Msa0022310 | Msa1435420 | 0.806887 | 6.612254e-50 | -8.615850e-47 |
| Msa0022310 | Msa1436480 | 0.806655 | 7.403367e-50 | -8.615850e-47 |
| Msa0022310 | Msa1437950 | 0.806899 | 6.573313e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0022310 | MtrunA17_Chr1g0175521 | 90.654 | 321 | 24 | 2 | 1 | 321 | 1 | 315 | 0.0 | 543 |
| Msa0022310 | MtrunA17_Chr7g0258791 | 48.459 | 357 | 129 | 13 | 1 | 321 | 1 | 338 | 4.61e-78 | 241 |
| Msa0022310 | MtrunA17_Chr7g0258801 | 48.802 | 334 | 133 | 10 | 12 | 321 | 23 | 342 | 6.40e-76 | 236 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0022310 | AT2G42040.1 | 54.630 | 108 | 21 | 3 | 203 | 310 | 163 | 242 | 1.30e-21 | 92.8 |
Find 88 sgRNAs with CRISPR-Local
Find 152 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTATAGAAGGATCTAAATT+TGG | 0.138899 | 1_1:+41759867 | None:intergenic |
| GCACAAGAAGCAAGAAATTT+TGG | 0.142499 | 1_1:-41759518 | Msa0022310:intron |
| AAGAATGCGTTGTTTGATTT+TGG | 0.169330 | 1_1:-41757624 | Msa0022310:three_prime_UTR |
| TCTCTATGTGAGCATCATTT+AGG | 0.194755 | 1_1:-41758149 | Msa0022310:CDS |
| CTTCCTCTCCGTTTCTTTAG+AGG | 0.209046 | 1_1:+41758535 | None:intergenic |
| TATTATTAAATTTAAGATAT+TGG | 0.229930 | 1_1:-41757650 | Msa0022310:three_prime_UTR |
| GTTCTTGGTTGGTTTCTATC+TGG | 0.254125 | 1_1:+41760053 | None:intergenic |
| CTAAATTTGGGTTTAGATTT+CGG | 0.256077 | 1_1:+41759880 | None:intergenic |
| GGAGGTTCAGTTTGAGATAT+TGG | 0.256335 | 1_1:+41758053 | None:intergenic |
| CTCTCACTAAGATATGTTCT+TGG | 0.279403 | 1_1:+41760038 | None:intergenic |
| TCGCGGTGCAGTCGAGTTAA+TGG | 0.280856 | 1_1:-41758212 | Msa0022310:CDS |
| CTCTATGTGAGCATCATTTA+GGG | 0.282907 | 1_1:-41758148 | Msa0022310:CDS |
| GGTAAGAGGTGGTGCACTTA+TGG | 0.318134 | 1_1:-41758240 | Msa0022310:CDS |
| GCATGACAAGTGTAAGAAAT+AGG | 0.329001 | 1_1:-41758109 | Msa0022310:CDS |
| CTATAGAAGGATCTAAATTT+GGG | 0.347816 | 1_1:+41759868 | None:intergenic |
| TTACCTTGTTTATGACTAGA+AGG | 0.355956 | 1_1:+41758690 | None:intergenic |
| AAATTTCTTGCTTCTTGTGC+TGG | 0.356796 | 1_1:+41759521 | None:intergenic |
| GAAAAGCTAACCAAGTTTGA+TGG | 0.358390 | 1_1:-41758023 | Msa0022310:CDS |
| TATTTCATGTACTTCAGATT+AGG | 0.378804 | 1_1:-41757823 | Msa0022310:three_prime_UTR |
| GCTCATAAATAACTAGACTT+TGG | 0.381860 | 1_1:+41759816 | None:intergenic |
| TGAGAGAAAGAAAGTGCATT+AGG | 0.387249 | 1_1:-41760021 | Msa0022310:five_prime_UTR |
| AGTACTAATCGCAAAATACT+AGG | 0.391185 | 1_1:-41757863 | Msa0022310:three_prime_UTR |
| TGAACTTGCACTCACGTATA+AGG | 0.423080 | 1_1:+41757775 | None:intergenic |
| CTCTTCTTCGCGATAATCAT+CGG | 0.437633 | 1_1:+41757978 | None:intergenic |
| CACTAAGATATGTTCTTGGT+TGG | 0.444824 | 1_1:+41760042 | None:intergenic |
| TCATGAGTGCAAGAATGTTT+TGG | 0.453295 | 1_1:+41759646 | None:intergenic |
| CAGATCTCAACATGATCAAC+CGG | 0.459291 | 1_1:-41759711 | Msa0022310:CDS |
| TTATGACTAGAAGGCCTTGA+AGG | 0.462959 | 1_1:+41758699 | None:intergenic |
| GCGATCGATGAGTAGTTTGT+TGG | 0.464541 | 1_1:-41757931 | Msa0022310:CDS |
| ATTGTCTTCACCATCAAACT+TGG | 0.466393 | 1_1:+41758013 | None:intergenic |
| TACTGCACTGTTATCTCTCT+CGG | 0.470852 | 1_1:-41759617 | Msa0022310:CDS |
| GCGAAGAAGAGGATGAAGAT+TGG | 0.473786 | 1_1:-41757966 | Msa0022310:CDS |
| TTTGTGTTGTAATTTCTTGC+GGG | 0.474320 | 1_1:-41757678 | Msa0022310:three_prime_UTR |
| AGAGGGCATGTCAAGGAAGG+GGG | 0.481397 | 1_1:-41759234 | Msa0022310:CDS |
| TTTAGTTTCATAAACTAGAT+GGG | 0.482660 | 1_1:-41759939 | Msa0022310:five_prime_UTR |
| GGAAGATCATAAAGAAGAAG+AGG | 0.484016 | 1_1:-41758410 | Msa0022310:CDS |
| GATCAACCGGTCACCGGTGG+TGG | 0.490122 | 1_1:-41759698 | Msa0022310:CDS |
| ACATTCTTGCACTCATGACT+TGG | 0.490947 | 1_1:-41759641 | Msa0022310:CDS |
| ATCAACTACTCCCACCGATC+GGG | 0.492964 | 1_1:-41759576 | Msa0022310:CDS |
| GAGAGGGCATGTCAAGGAAG+GGG | 0.502321 | 1_1:-41759235 | Msa0022310:CDS |
| GGGAGAGGGCATGTCAAGGA+AGG | 0.508157 | 1_1:-41759237 | Msa0022310:CDS |
| TAGAGAAACAAAACAAAAGA+AGG | 0.509554 | 1_1:-41758377 | Msa0022310:intron |
| AGTGTTATGATAGTAATGAA+TGG | 0.510545 | 1_1:-41758441 | Msa0022310:CDS |
| TATAGTATCTTGCAACTATG+AGG | 0.511241 | 1_1:-41759759 | Msa0022310:exon |
| CCGTTGGCTTGTTTCCCGAT+CGG | 0.511460 | 1_1:+41759562 | None:intergenic |
| TCATTTATATAGATTGGAGA+TGG | 0.513170 | 1_1:-41758579 | Msa0022310:intron |
| CGATCGATGAGTAGTTTGTT+GGG | 0.513939 | 1_1:-41757930 | Msa0022310:CDS |
| CGATGATTATCGCGAAGAAG+AGG | 0.515105 | 1_1:-41757977 | Msa0022310:CDS |
| TCATAAAGAAGAAGAGGTAG+TGG | 0.518196 | 1_1:-41758404 | Msa0022310:CDS |
| TTGGCTTGTTTCCCGATCGG+TGG | 0.518762 | 1_1:+41759565 | None:intergenic |
| GTTTGTGTTGTAATTTCTTG+CGG | 0.519726 | 1_1:-41757679 | Msa0022310:three_prime_UTR |
| TGGCTTGTTTCCCGATCGGT+GGG | 0.519826 | 1_1:+41759566 | None:intergenic |
| TGCAGTCGAGTTAATGGAAG+AGG | 0.520583 | 1_1:-41758206 | Msa0022310:CDS |
| TACTGAATCATCAGAGCCGT+TGG | 0.530770 | 1_1:+41759546 | None:intergenic |
| GTTGCACCACCACCGGTGAC+CGG | 0.532197 | 1_1:+41759692 | None:intergenic |
| AGCATCATTTAGGGAAAGGA+AGG | 0.554136 | 1_1:-41758139 | Msa0022310:CDS |
| TGTGATTTGCAGGTAGAAGA+TGG | 0.559441 | 1_1:-41759258 | Msa0022310:intron |
| GGAGAGGGCATGTCAAGGAA+GGG | 0.559602 | 1_1:-41759236 | Msa0022310:CDS |
| TAGTAATGAATGGGATGAAG+AGG | 0.560430 | 1_1:-41758431 | Msa0022310:CDS |
| GTACTAATCGCAAAATACTA+GGG | 0.561295 | 1_1:-41757862 | Msa0022310:three_prime_UTR |
| GTGTTATGATAGTAATGAAT+GGG | 0.561410 | 1_1:-41758440 | Msa0022310:CDS |
| TGTAAGAAATAGGTCTATTG+TGG | 0.562415 | 1_1:-41758099 | Msa0022310:CDS |
| TCGTTGAGTTGCACCACCAC+CGG | 0.564805 | 1_1:+41759685 | None:intergenic |
| TGTGAGCATCATTTAGGGAA+AGG | 0.571914 | 1_1:-41758143 | Msa0022310:CDS |
| GATCAACTACTCCCACCGAT+CGG | 0.572727 | 1_1:-41759577 | Msa0022310:CDS |
| GAGTTAATGGAAGAGGATGG+AGG | 0.574908 | 1_1:-41758199 | Msa0022310:CDS |
| GAGTGCACAAACTTCAACTG+AGG | 0.575039 | 1_1:-41758730 | Msa0022310:CDS |
| CAACATGATCAACCGGTCAC+CGG | 0.577249 | 1_1:-41759704 | Msa0022310:CDS |
| GTGATTTGCAGGTAGAAGAT+GGG | 0.581315 | 1_1:-41759257 | Msa0022310:intron |
| AGTAGTTGATCAGACGGCTG+AGG | 0.594515 | 1_1:+41759589 | None:intergenic |
| TTGCAGGTAGAAGATGGGAG+AGG | 0.602099 | 1_1:-41759252 | Msa0022310:intron |
| GAAGAGGATGAAGATTGGAA+TGG | 0.603128 | 1_1:-41757961 | Msa0022310:CDS |
| GTGCACTTATGGAAGGTTCG+CGG | 0.606666 | 1_1:-41758229 | Msa0022310:CDS |
| AGAGAATAACCGACTAGTGT+TGG | 0.608366 | 1_1:+41758167 | None:intergenic |
| TGCAGGTAGAAGATGGGAGA+GGG | 0.613399 | 1_1:-41759251 | Msa0022310:intron |
| GTCGAGTTAATGGAAGAGGA+TGG | 0.615801 | 1_1:-41758202 | Msa0022310:CDS |
| AGATGGGAGAGGGCATGTCA+AGG | 0.620023 | 1_1:-41759241 | Msa0022310:CDS |
| AGAGGTGGTGCACTTATGGA+AGG | 0.620926 | 1_1:-41758236 | Msa0022310:CDS |
| GGTGGGAGTAGTTGATCAGA+CGG | 0.626321 | 1_1:+41759583 | None:intergenic |
| ACAAACAATCAGTGATCTCG+TGG | 0.632907 | 1_1:-41757907 | Msa0022310:CDS |
| GACAAAAGGGTAATGATACC+AGG | 0.635672 | 1_1:-41759212 | Msa0022310:intron |
| CATGATCAACCGGTCACCGG+TGG | 0.646362 | 1_1:-41759701 | Msa0022310:CDS |
| TGTTGTCAACCAACACTAGT+CGG | 0.655661 | 1_1:-41758176 | Msa0022310:CDS |
| AGGCCTTCTAGTCATAAACA+AGG | 0.659693 | 1_1:-41758693 | Msa0022310:intron |
| TTTCCTCTAAAGAAACGGAG+AGG | 0.668761 | 1_1:-41758538 | Msa0022310:CDS |
| TTGGAGATGGAACGACGGAG+AGG | 0.683107 | 1_1:-41758566 | Msa0022310:CDS |
| CCGATCGGGAAACAAGCCAA+CGG | 0.690535 | 1_1:-41759562 | Msa0022310:CDS |
| ATAGATTGGAGATGGAACGA+CGG | 0.693875 | 1_1:-41758571 | Msa0022310:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTTTTAATTTTTTTTT+AGG | - | chr1_1:41758212-41758231 | Msa0022310:CDS | 0.0% |
| !! | TATTAATGTGTTATATTTAT+TGG | - | chr1_1:41759009-41759028 | Msa0022310:intron | 10.0% |
| !! | TAAAATCAAAATAAAAAAGA+AGG | - | chr1_1:41759413-41759432 | Msa0022310:intron | 10.0% |
| !!! | ATTTTATTTCACATTCATAA+AGG | - | chr1_1:41758300-41758319 | Msa0022310:intron | 15.0% |
| !!! | TTTTATTTCACATTCATAAA+GGG | - | chr1_1:41758301-41758320 | Msa0022310:intron | 15.0% |
| !! | AATATCTCATAGTAAAAAAA+AGG | - | chr1_1:41758526-41758545 | Msa0022310:CDS | 15.0% |
| !!! | GATTTTATAAACATCATATA+AGG | + | chr1_1:41759400-41759419 | None:intergenic | 15.0% |
| !!! | TTTTAGTTTCATAAACTAGA+TGG | - | chr1_1:41757734-41757753 | Msa0022310:three_prime_UTR | 20.0% |
| !!! | TTTAGTTTCATAAACTAGAT+GGG | - | chr1_1:41757735-41757754 | Msa0022310:three_prime_UTR | 20.0% |
| !! | TAAAAATCACATACTATAGA+AGG | + | chr1_1:41757822-41757841 | None:intergenic | 20.0% |
| !! | TTTATTTCACATTCATAAAG+GGG | - | chr1_1:41758302-41758321 | Msa0022310:intron | 20.0% |
| !!! | AATATTTTTGAAAGACACAA+AGG | - | chr1_1:41758326-41758345 | Msa0022310:intron | 20.0% |
| !!! | ATATTTTTGAAAGACACAAA+GGG | - | chr1_1:41758327-41758346 | Msa0022310:intron | 20.0% |
| !!! | TTTTTACTATGAGATATTCA+TGG | + | chr1_1:41758524-41758543 | None:intergenic | 20.0% |
| !! | AAAAAAGATGTGATGTAAAT+GGG | + | chr1_1:41758805-41758824 | None:intergenic | 20.0% |
| !! | AAAAAAAGATGTGATGTAAA+TGG | + | chr1_1:41758806-41758825 | None:intergenic | 20.0% |
| !!! | CTATTGTCATTTATATAGAT+TGG | - | chr1_1:41759089-41759108 | Msa0022310:intron | 20.0% |
| !! | AAAATAAAAAAGAAGGTAAG+AGG | - | chr1_1:41759420-41759439 | Msa0022310:intron | 20.0% |
| !! | CTAAATTTGGGTTTAGATTT+CGG | + | chr1_1:41757797-41757816 | None:intergenic | 25.0% |
| ! | CTATAGAAGGATCTAAATTT+GGG | + | chr1_1:41757809-41757828 | None:intergenic | 25.0% |
| ! | ACTATAGAAGGATCTAAATT+TGG | + | chr1_1:41757810-41757829 | None:intergenic | 25.0% |
| !!! | TATTTTTGAAAGACACAAAG+GGG | - | chr1_1:41758328-41758347 | Msa0022310:intron | 25.0% |
| !! | ACCAACCATATTAATTTTAG+TGG | - | chr1_1:41758362-41758381 | Msa0022310:intron | 25.0% |
| ! | TCCACTAAAATTAATATGGT+TGG | + | chr1_1:41758366-41758385 | None:intergenic | 25.0% |
| ! | AGGATCCACTAAAATTAATA+TGG | + | chr1_1:41758370-41758389 | None:intergenic | 25.0% |
| ! | CACAAAAAAGAAACATACAT+AGG | + | chr1_1:41758390-41758409 | None:intergenic | 25.0% |
| ! | TAATTTCATGTGTCTTTCTT+TGG | + | chr1_1:41758493-41758512 | None:intergenic | 25.0% |
| ! | GTAAAAAAAAGGTGAAAACA+TGG | - | chr1_1:41758537-41758556 | Msa0022310:CDS | 25.0% |
| !!! | AACTATATGTTTTTTCATGC+CGG | + | chr1_1:41758583-41758602 | None:intergenic | 25.0% |
| !!! | AGTTTTGAGAAAAAAACGAT+CGG | + | chr1_1:41758716-41758735 | None:intergenic | 25.0% |
| ! | AAAAAGATGTGATGTAAATG+GGG | + | chr1_1:41758804-41758823 | None:intergenic | 25.0% |
| ! | ACTTCAAGGTAAAAAAGAAA+AGG | + | chr1_1:41758854-41758873 | None:intergenic | 25.0% |
| ! | TCATTTATATAGATTGGAGA+TGG | - | chr1_1:41759095-41759114 | Msa0022310:intron | 25.0% |
| ! | AGTGTTATGATAGTAATGAA+TGG | - | chr1_1:41759233-41759252 | Msa0022310:CDS | 25.0% |
| ! | GTGTTATGATAGTAATGAAT+GGG | - | chr1_1:41759234-41759253 | Msa0022310:CDS | 25.0% |
| ! | TAGAGAAACAAAACAAAAGA+AGG | - | chr1_1:41759297-41759316 | Msa0022310:intron | 25.0% |
| ! | TATTTCATGTACTTCAGATT+AGG | - | chr1_1:41759851-41759870 | Msa0022310:five_prime_UTR | 25.0% |
| !! | GTTCATATTTTGTGAAGTTT+TGG | - | chr1_1:41759973-41759992 | Msa0022310:five_prime_UTR | 25.0% |
| GCTCATAAATAACTAGACTT+TGG | + | chr1_1:41757861-41757880 | None:intergenic | 30.0% | |
| TATAGTATCTTGCAACTATG+AGG | - | chr1_1:41757915-41757934 | Msa0022310:CDS | 30.0% | |
| TCTAACACTTTGTAAAAGGT+TGG | + | chr1_1:41758269-41758288 | None:intergenic | 30.0% | |
| CCAATCTAACACTTTGTAAA+AGG | + | chr1_1:41758273-41758292 | None:intergenic | 30.0% | |
| !! | CCTTTTACAAAGTGTTAGAT+TGG | - | chr1_1:41758270-41758289 | Msa0022310:intron | 30.0% |
| TTACTATGAGATATTCATGG+AGG | + | chr1_1:41758521-41758540 | None:intergenic | 30.0% | |
| AAAAGATGTGATGTAAATGG+GGG | + | chr1_1:41758803-41758822 | None:intergenic | 30.0% | |
| !!! | CTTTTTTACCTTGAAGTGTA+TGG | - | chr1_1:41758857-41758876 | Msa0022310:intron | 30.0% |
| TTGTGTGGTATATATGTTTG+TGG | - | chr1_1:41758885-41758904 | Msa0022310:intron | 30.0% | |
| !!! | GATGTGTCTTTTTTGTTAGT+AGG | - | chr1_1:41758907-41758926 | Msa0022310:intron | 30.0% |
| !!! | GTCTTTTTTGTTAGTAGGAA+TGG | - | chr1_1:41758912-41758931 | Msa0022310:intron | 30.0% |
| ! | TTACCTTGTTTATGACTAGA+AGG | + | chr1_1:41758987-41759006 | None:intergenic | 30.0% |
| !! | AAGCTTTTCCTCTAAAGAAA+CGG | - | chr1_1:41759131-41759150 | Msa0022310:intron | 30.0% |
| ATAAAAAAGAAGGTAAGAGG+TGG | - | chr1_1:41759423-41759442 | Msa0022310:intron | 30.0% | |
| TGTAAGAAATAGGTCTATTG+TGG | - | chr1_1:41759575-41759594 | Msa0022310:CDS | 30.0% | |
| AGTACTAATCGCAAAATACT+AGG | - | chr1_1:41759811-41759830 | Msa0022310:five_prime_UTR | 30.0% | |
| GTACTAATCGCAAAATACTA+GGG | - | chr1_1:41759812-41759831 | Msa0022310:five_prime_UTR | 30.0% | |
| GTTTGTGTTGTAATTTCTTG+CGG | - | chr1_1:41759995-41760014 | Msa0022310:five_prime_UTR | 30.0% | |
| TTTGTGTTGTAATTTCTTGC+GGG | - | chr1_1:41759996-41760015 | Msa0022310:five_prime_UTR | 30.0% | |
| !! | AAGAATGCGTTGTTTGATTT+TGG | - | chr1_1:41760050-41760069 | Msa0022310:five_prime_UTR | 30.0% |
| CACTAAGATATGTTCTTGGT+TGG | + | chr1_1:41757635-41757654 | None:intergenic | 35.0% | |
| CTCTCACTAAGATATGTTCT+TGG | + | chr1_1:41757639-41757658 | None:intergenic | 35.0% | |
| ! | TGAGAGAAAGAAAGTGCATT+AGG | - | chr1_1:41757653-41757672 | Msa0022310:three_prime_UTR | 35.0% |
| ! | AGAGAGTAGAGTAAGCTTTT+CGG | + | chr1_1:41757943-41757962 | None:intergenic | 35.0% |
| ! | TCATGAGTGCAAGAATGTTT+TGG | + | chr1_1:41758031-41758050 | None:intergenic | 35.0% |
| AAATTTCTTGCTTCTTGTGC+TGG | + | chr1_1:41758156-41758175 | None:intergenic | 35.0% | |
| ! | GCACAAGAAGCAAGAAATTT+TGG | - | chr1_1:41758156-41758175 | Msa0022310:CDS | 35.0% |
| !!! | TGTCTTTCTTTGGTTTTACC+TGG | + | chr1_1:41758483-41758502 | None:intergenic | 35.0% |
| GACCCATAACTTGATTTAAC+CGG | - | chr1_1:41758561-41758580 | Msa0022310:CDS | 35.0% | |
| ! | TGCCGGTTAAATCAAGTTAT+GGG | + | chr1_1:41758566-41758585 | None:intergenic | 35.0% |
| ! | ATGCCGGTTAAATCAAGTTA+TGG | + | chr1_1:41758567-41758586 | None:intergenic | 35.0% |
| ! | AACGATCGGTTTAGATAAGA+GGG | + | chr1_1:41758702-41758721 | None:intergenic | 35.0% |
| ! | AAACGATCGGTTTAGATAAG+AGG | + | chr1_1:41758703-41758722 | None:intergenic | 35.0% |
| CAATTACACCATACACTTCA+AGG | + | chr1_1:41758868-41758887 | None:intergenic | 35.0% | |
| AAGTGTATGGTGTAATTGTG+TGG | - | chr1_1:41758870-41758889 | Msa0022310:intron | 35.0% | |
| TAGTAATGAATGGGATGAAG+AGG | - | chr1_1:41759243-41759262 | Msa0022310:CDS | 35.0% | |
| GGAAGATCATAAAGAAGAAG+AGG | - | chr1_1:41759264-41759283 | Msa0022310:intron | 35.0% | |
| TCATAAAGAAGAAGAGGTAG+TGG | - | chr1_1:41759270-41759289 | Msa0022310:intron | 35.0% | |
| TCTCTATGTGAGCATCATTT+AGG | - | chr1_1:41759525-41759544 | Msa0022310:CDS | 35.0% | |
| CTCTATGTGAGCATCATTTA+GGG | - | chr1_1:41759526-41759545 | Msa0022310:CDS | 35.0% | |
| GCATGACAAGTGTAAGAAAT+AGG | - | chr1_1:41759565-41759584 | Msa0022310:CDS | 35.0% | |
| !!! | TTGGTTAGCTTTTCAAGAGA+TGG | + | chr1_1:41759645-41759664 | None:intergenic | 35.0% |
| GAAAAGCTAACCAAGTTTGA+TGG | - | chr1_1:41759651-41759670 | Msa0022310:CDS | 35.0% | |
| ATTGTCTTCACCATCAAACT+TGG | + | chr1_1:41759664-41759683 | None:intergenic | 35.0% | |
| !! | GTTCTTGGTTGGTTTCTATC+TGG | + | chr1_1:41757624-41757643 | None:intergenic | 40.0% |
| CAGATCTCAACATGATCAAC+CGG | - | chr1_1:41757963-41757982 | Msa0022310:CDS | 40.0% | |
| ACATTCTTGCACTCATGACT+TGG | - | chr1_1:41758033-41758052 | Msa0022310:CDS | 40.0% | |
| TACTGCACTGTTATCTCTCT+CGG | - | chr1_1:41758057-41758076 | Msa0022310:CDS | 40.0% | |
| ! | TGTGATTTGCAGGTAGAAGA+TGG | - | chr1_1:41758416-41758435 | Msa0022310:CDS | 40.0% |
| ! | GTGATTTGCAGGTAGAAGAT+GGG | - | chr1_1:41758417-41758436 | Msa0022310:CDS | 40.0% |
| GACAAAAGGGTAATGATACC+AGG | - | chr1_1:41758462-41758481 | Msa0022310:CDS | 40.0% | |
| TTATGACTAGAAGGCCTTGA+AGG | + | chr1_1:41758978-41758997 | None:intergenic | 40.0% | |
| AGGCCTTCTAGTCATAAACA+AGG | - | chr1_1:41758981-41759000 | Msa0022310:intron | 40.0% | |
| ATAGATTGGAGATGGAACGA+CGG | - | chr1_1:41759103-41759122 | Msa0022310:intron | 40.0% | |
| ! | TTTCCTCTAAAGAAACGGAG+AGG | - | chr1_1:41759136-41759155 | Msa0022310:intron | 40.0% |
| TGTTGTCAACCAACACTAGT+CGG | - | chr1_1:41759498-41759517 | Msa0022310:intron | 40.0% | |
| AGAGAATAACCGACTAGTGT+TGG | + | chr1_1:41759510-41759529 | None:intergenic | 40.0% | |
| TGTGAGCATCATTTAGGGAA+AGG | - | chr1_1:41759531-41759550 | Msa0022310:CDS | 40.0% | |
| AGCATCATTTAGGGAAAGGA+AGG | - | chr1_1:41759535-41759554 | Msa0022310:CDS | 40.0% | |
| ! | GGAGGTTCAGTTTGAGATAT+TGG | + | chr1_1:41759624-41759643 | None:intergenic | 40.0% |
| !!! | GTTAGCTTTTCAAGAGATGG+AGG | + | chr1_1:41759642-41759661 | None:intergenic | 40.0% |
| CTCTTCTTCGCGATAATCAT+CGG | + | chr1_1:41759699-41759718 | None:intergenic | 40.0% | |
| GAAGAGGATGAAGATTGGAA+TGG | - | chr1_1:41759713-41759732 | Msa0022310:CDS | 40.0% | |
| !! | CGATCGATGAGTAGTTTGTT+GGG | - | chr1_1:41759744-41759763 | Msa0022310:CDS | 40.0% |
| ACAAACAATCAGTGATCTCG+TGG | - | chr1_1:41759767-41759786 | Msa0022310:five_prime_UTR | 40.0% | |
| TGAACTTGCACTCACGTATA+AGG | + | chr1_1:41759902-41759921 | None:intergenic | 40.0% | |
| TACTGAATCATCAGAGCCGT+TGG | + | chr1_1:41758131-41758150 | None:intergenic | 45.0% | |
| CAGCTAAAGGGCAAATTGGT+AGG | + | chr1_1:41758744-41758763 | None:intergenic | 45.0% | |
| TGTGCAGCTAAAGGGCAAAT+TGG | + | chr1_1:41758748-41758767 | None:intergenic | 45.0% | |
| TACCTGCTTGTGCAGCTAAA+GGG | + | chr1_1:41758756-41758775 | None:intergenic | 45.0% | |
| GAGTGCACAAACTTCAACTG+AGG | - | chr1_1:41758944-41758963 | Msa0022310:intron | 45.0% | |
| ! | CTGAGGTTTTCTCTCCTTCA+AGG | - | chr1_1:41758961-41758980 | Msa0022310:intron | 45.0% |
| CTTCCTCTCCGTTTCTTTAG+AGG | + | chr1_1:41759142-41759161 | None:intergenic | 45.0% | |
| TGCAGTCGAGTTAATGGAAG+AGG | - | chr1_1:41759468-41759487 | Msa0022310:intron | 45.0% | |
| GTCGAGTTAATGGAAGAGGA+TGG | - | chr1_1:41759472-41759491 | Msa0022310:intron | 45.0% | |
| GAGTTAATGGAAGAGGATGG+AGG | - | chr1_1:41759475-41759494 | Msa0022310:intron | 45.0% | |
| CGATGATTATCGCGAAGAAG+AGG | - | chr1_1:41759697-41759716 | Msa0022310:CDS | 45.0% | |
| GCGAAGAAGAGGATGAAGAT+TGG | - | chr1_1:41759708-41759727 | Msa0022310:CDS | 45.0% | |
| ! | GCGATCGATGAGTAGTTTGT+TGG | - | chr1_1:41759743-41759762 | Msa0022310:CDS | 45.0% |
| !!! | TATTTTTTTATGAAATTATA+AGG | - | chr1_1:41758607-41758626 | Msa0022310:intron | 5.0% |
| !!! | TATTATTAAATTTAAGATAT+TGG | - | chr1_1:41760024-41760043 | Msa0022310:five_prime_UTR | 5.0% |
| CAACATGATCAACCGGTCAC+CGG | - | chr1_1:41757970-41757989 | Msa0022310:CDS | 50.0% | |
| AGTAGTTGATCAGACGGCTG+AGG | + | chr1_1:41758088-41758107 | None:intergenic | 50.0% | |
| GGTGGGAGTAGTTGATCAGA+CGG | + | chr1_1:41758094-41758113 | None:intergenic | 50.0% | |
| GATCAACTACTCCCACCGAT+CGG | - | chr1_1:41758097-41758116 | Msa0022310:CDS | 50.0% | |
| ATCAACTACTCCCACCGATC+GGG | - | chr1_1:41758098-41758117 | Msa0022310:CDS | 50.0% | |
| TTGCAGGTAGAAGATGGGAG+AGG | - | chr1_1:41758422-41758441 | Msa0022310:CDS | 50.0% | |
| TGCAGGTAGAAGATGGGAGA+GGG | - | chr1_1:41758423-41758442 | Msa0022310:CDS | 50.0% | |
| TGTCAAGGAAGGGGGACAAA+AGG | - | chr1_1:41758448-41758467 | Msa0022310:CDS | 50.0% | |
| GTCAAGGAAGGGGGACAAAA+GGG | - | chr1_1:41758449-41758468 | Msa0022310:CDS | 50.0% | |
| CTACCTGCTTGTGCAGCTAA+AGG | + | chr1_1:41758757-41758776 | None:intergenic | 50.0% | |
| GATCGAGTGGAACCAAATGC+TGG | - | chr1_1:41758776-41758795 | Msa0022310:intron | 50.0% | |
| GTAAATGGGGGACCAGCATT+TGG | + | chr1_1:41758791-41758810 | None:intergenic | 50.0% | |
| GGTAAGAGGTGGTGCACTTA+TGG | - | chr1_1:41759434-41759453 | Msa0022310:intron | 50.0% | |
| AGAGGTGGTGCACTTATGGA+AGG | - | chr1_1:41759438-41759457 | Msa0022310:intron | 50.0% | |
| GTGCACTTATGGAAGGTTCG+CGG | - | chr1_1:41759445-41759464 | Msa0022310:intron | 50.0% | |
| ! | TCGTTGAGTTGCACCACCAC+CGG | + | chr1_1:41757992-41758011 | None:intergenic | 55.0% |
| !! | TGGCTTGTTTCCCGATCGGT+GGG | + | chr1_1:41758111-41758130 | None:intergenic | 55.0% |
| !! | TTGGCTTGTTTCCCGATCGG+TGG | + | chr1_1:41758112-41758131 | None:intergenic | 55.0% |
| !! | CCGTTGGCTTGTTTCCCGAT+CGG | + | chr1_1:41758115-41758134 | None:intergenic | 55.0% |
| CCGATCGGGAAACAAGCCAA+CGG | - | chr1_1:41758112-41758131 | Msa0022310:CDS | 55.0% | |
| ! | TGTGCGTGCGTGTGATTTGC+AGG | - | chr1_1:41758406-41758425 | Msa0022310:CDS | 55.0% |
| ! | AGATGGGAGAGGGCATGTCA+AGG | - | chr1_1:41758433-41758452 | Msa0022310:CDS | 55.0% |
| ! | GGAGAGGGCATGTCAAGGAA+GGG | - | chr1_1:41758438-41758457 | Msa0022310:CDS | 55.0% |
| ! | GAGAGGGCATGTCAAGGAAG+GGG | - | chr1_1:41758439-41758458 | Msa0022310:CDS | 55.0% |
| ! | AGAGGGCATGTCAAGGAAGG+GGG | - | chr1_1:41758440-41758459 | Msa0022310:CDS | 55.0% |
| TGCCCTTTAGCTGCACAAGC+AGG | - | chr1_1:41758751-41758770 | Msa0022310:intron | 55.0% | |
| GCACAAGCAGGTAGATCGAG+TGG | - | chr1_1:41758763-41758782 | Msa0022310:intron | 55.0% | |
| TTGGAGATGGAACGACGGAG+AGG | - | chr1_1:41759108-41759127 | Msa0022310:intron | 55.0% | |
| TCGCGGTGCAGTCGAGTTAA+TGG | - | chr1_1:41759462-41759481 | Msa0022310:intron | 55.0% | |
| CATGATCAACCGGTCACCGG+TGG | - | chr1_1:41757973-41757992 | Msa0022310:CDS | 60.0% | |
| ! | GGGAGAGGGCATGTCAAGGA+AGG | - | chr1_1:41758437-41758456 | Msa0022310:CDS | 60.0% |
| GATCAACCGGTCACCGGTGG+TGG | - | chr1_1:41757976-41757995 | Msa0022310:CDS | 65.0% | |
| GTTGCACCACCACCGGTGAC+CGG | + | chr1_1:41757985-41758004 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 41757594 | 41760102 | 41757594 | ID=Msa0022310;Name=Msa0022310 |
| chr1_1 | mRNA | 41757594 | 41760102 | 41757594 | ID=Msa0022310-mRNA-1;Parent=Msa0022310;Name=Msa0022310-mRNA-1;_AED=0.10;_eAED=0.10;_QI=338|1|1|1|1|1|5|293|321 |
| chr1_1 | exon | 41757594 | 41758264 | 41757594 | ID=Msa0022310-mRNA-1:exon:12320;Parent=Msa0022310-mRNA-1 |
| chr1_1 | exon | 41758378 | 41758589 | 41758378 | ID=Msa0022310-mRNA-1:exon:12319;Parent=Msa0022310-mRNA-1 |
| chr1_1 | exon | 41758694 | 41758767 | 41758694 | ID=Msa0022310-mRNA-1:exon:12318;Parent=Msa0022310-mRNA-1 |
| chr1_1 | exon | 41759213 | 41759268 | 41759213 | ID=Msa0022310-mRNA-1:exon:12317;Parent=Msa0022310-mRNA-1 |
| chr1_1 | exon | 41759519 | 41760102 | 41759519 | ID=Msa0022310-mRNA-1:exon:12316;Parent=Msa0022310-mRNA-1 |
| chr1_1 | five_prime_UTR | 41759765 | 41760102 | 41759765 | ID=Msa0022310-mRNA-1:five_prime_utr;Parent=Msa0022310-mRNA-1 |
| chr1_1 | CDS | 41759519 | 41759764 | 41759519 | ID=Msa0022310-mRNA-1:cds;Parent=Msa0022310-mRNA-1 |
| chr1_1 | CDS | 41759213 | 41759268 | 41759213 | ID=Msa0022310-mRNA-1:cds;Parent=Msa0022310-mRNA-1 |
| chr1_1 | CDS | 41758694 | 41758767 | 41758694 | ID=Msa0022310-mRNA-1:cds;Parent=Msa0022310-mRNA-1 |
| chr1_1 | CDS | 41758378 | 41758589 | 41758378 | ID=Msa0022310-mRNA-1:cds;Parent=Msa0022310-mRNA-1 |
| chr1_1 | CDS | 41757887 | 41758264 | 41757887 | ID=Msa0022310-mRNA-1:cds;Parent=Msa0022310-mRNA-1 |
| chr1_1 | three_prime_UTR | 41757594 | 41757886 | 41757594 | ID=Msa0022310-mRNA-1:three_prime_utr;Parent=Msa0022310-mRNA-1 |
| Gene Sequence |
| Protein sequence |
