AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67232


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67232.t1 MTR_8g088720 97.297 148 4 0 1 148 1 148 7.18e-107 301
MS.gene67232.t1 MTR_2g006290 63.816 152 44 3 4 148 3 150 1.87e-63 191
MS.gene67232.t1 MTR_1g112030 56.164 146 57 5 4 146 18 159 1.57e-50 159
MS.gene67232.t1 MTR_1g112030 56.164 146 57 5 4 146 14 155 1.65e-50 159
MS.gene67232.t1 MTR_1g112050 55.782 147 62 3 1 146 9 153 6.20e-50 157
MS.gene67232.t1 MTR_2g086510 61.789 123 45 2 25 146 33 154 1.05e-49 156
MS.gene67232.t1 MTR_4g053120 57.037 135 51 3 19 146 20 154 5.77e-48 152
MS.gene67232.t1 MTR_5g006320 36.634 101 63 1 48 148 46 145 1.67e-17 74.7
MS.gene67232.t1 MTR_4g098490 36.585 82 52 0 65 146 77 158 3.29e-16 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67232.t1 AT1G60950 64.000 150 49 3 1 147 1 148 1.03e-65 197
MS.gene67232.t1 AT1G10960 61.333 150 53 3 1 147 1 148 5.54e-59 180
MS.gene67232.t1 AT2G27510 50.658 152 65 4 5 146 3 154 1.99e-44 143
MS.gene67232.t1 AT5G10000 43.182 132 71 3 19 147 18 148 1.23e-32 113
MS.gene67232.t1 AT4G14890 35.333 150 80 4 3 148 10 146 7.54e-17 72.8
MS.gene67232.t1 AT1G32550 40.244 82 49 0 65 146 72 153 1.26e-16 73.2
MS.gene67232.t1 AT1G32550 34.783 92 44 2 71 146 75 166 5.22e-12 60.8

Find 56 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGTGGGTCAGACTTTATT+TGG 0.300570 4.2:+12595888 MS.gene67232:CDS
CCACGTTAGGGAATGCTGTT+AGG 0.301806 4.2:-12595872 None:intergenic
GTAAGTTCTTTAGTTCCATC+TGG 0.311839 4.2:-12595975 None:intergenic
GCTGTTAGGGTTGTGTTCAT+TGG 0.358114 4.2:-12595858 None:intergenic
ACCACCACAGCAGCCCTATC+AGG 0.384793 4.2:+12595801 MS.gene67232:CDS
TGCTCACCATGGTTCCTGAT+AGG 0.397541 4.2:-12595815 None:intergenic
TGTGCTGGAAAAGTTGTAGA+AGG 0.411785 4.2:+12596086 MS.gene67232:CDS
AATAAAGTCTGACCCACGTT+AGG 0.420347 4.2:-12595885 None:intergenic
TCGAGAATGTACTCATTATC+CGG 0.426007 4.2:-12596002 None:intergenic
GATGAGCTTGATCTTGTAAC+TGG 0.426566 4.2:-12595949 None:intergenic
CTTCCTTACTCATGCAGGGC+TGG 0.437541 4.2:+12596050 MS.gene67232:CDS
GGTGCTTGCTCTTCATGTGC+TGG 0.439901 4.2:+12596071 MS.gene67232:CDS
TCGATCTTCCTTACTCATGC+AGG 0.452165 4.2:+12596045 MS.gene67232:CDS
TAAGTTCTTTAGTTCCATCT+GGG 0.455484 4.2:-12595974 None:intergenic
GCTCACCATGGTTCCTGATA+GGG 0.466904 4.2:-12595814 None:intergenic
TCCTGATAGGGCTGCTGTGG+TGG 0.470793 4.2:-12595802 None:intergenic
CATTGGCTGCCTCCTGATGA+AGG 0.472321 4.2:-12595841 None:intergenic
ACTTTATTTGGTGTGAAGGC+CGG 0.475879 4.2:+12595900 MS.gene67232:CDS
TGATAGGGCTGCTGTGGTGG+TGG 0.476979 4.2:-12595799 None:intergenic
TGGTGAGCACCTCCTTCATC+AGG 0.483290 4.2:+12595829 MS.gene67232:CDS
CACGTTAGGGAATGCTGTTA+GGG 0.501804 4.2:-12595871 None:intergenic
GCACCAGCCCTGCATGAGTA+AGG 0.502602 4.2:-12596053 None:intergenic
GATGATGATCAATTAAGTGC+TGG 0.507761 4.2:+12596140 MS.gene67232:CDS
TCAGACTTTATTTGGTGTGA+AGG 0.514081 4.2:+12595896 MS.gene67232:CDS
ATAAAGTCTGACCCACGTTA+GGG 0.523812 4.2:-12595884 None:intergenic
AAAAGTTGTAGAAGGGGAAG+TGG 0.534366 4.2:+12596094 MS.gene67232:CDS
ATCAAGCTCATCACCCCAGA+TGG 0.540257 4.2:+12595960 MS.gene67232:CDS
CTTGATCTTGTAACTGGCCA+TGG 0.544201 4.2:-12595943 None:intergenic
ATGATGATCAATTAAGTGCT+GGG 0.551795 4.2:+12596141 MS.gene67232:CDS
AAGATCGATGCCTTGTTCCT+CGG 0.553403 4.2:-12596030 None:intergenic
CTCGACGTAGCCGAGGAACA+AGG 0.555180 4.2:+12596020 MS.gene67232:CDS
CACTTAATTGATCATCATCA+AGG 0.556300 4.2:-12596136 None:intergenic
AACTAAAGAACTTACCTGCC+CGG 0.559896 4.2:+12595983 MS.gene67232:CDS
TTTGGTGTGAAGGCCGGACG+TGG 0.560810 4.2:+12595906 MS.gene67232:CDS
AATGTACTCATTATCCGGGC+AGG 0.566913 4.2:-12595997 None:intergenic
CGATCTTCCTTACTCATGCA+GGG 0.578511 4.2:+12596046 MS.gene67232:CDS
GTGCTGGAAAAGTTGTAGAA+GGG 0.579438 4.2:+12596087 MS.gene67232:CDS
GGTTCCTGATAGGGCTGCTG+TGG 0.579627 4.2:-12595805 None:intergenic
AGTGATACGACTTCCACGTC+CGG 0.584494 4.2:-12595919 None:intergenic
CGAGAATGTACTCATTATCC+GGG 0.584894 4.2:-12596001 None:intergenic
CCTTGTGTGTCTCAATAACA+AGG 0.591064 4.2:-12596199 None:intergenic
CCTAACAGCATTCCCTAACG+TGG 0.597665 4.2:+12595872 MS.gene67232:CDS
TGCTGGAAAAGTTGTAGAAG+GGG 0.598351 4.2:+12596088 MS.gene67232:CDS
TGGAAGTCGTATCACTGCCA+TGG 0.614707 4.2:+12595926 MS.gene67232:CDS
GTACATTCTCGACGTAGCCG+AGG 0.630338 4.2:+12596013 MS.gene67232:CDS
AAGTTCTTTAGTTCCATCTG+GGG 0.632400 4.2:-12595973 None:intergenic
AGCAGCCCTATCAGGAACCA+TGG 0.637647 4.2:+12595809 MS.gene67232:CDS
TGGCTGCCTCCTGATGAAGG+AGG 0.644050 4.2:-12595838 None:intergenic
GTGTGTCTCAATAACAAGGT+CGG 0.646026 4.2:-12596195 None:intergenic
CCTTGTTATTGAGACACACA+AGG 0.649100 4.2:+12596199 MS.gene67232:CDS
GATGAAGGAGGTGCTCACCA+TGG 0.650251 4.2:-12595826 None:intergenic
TCAATAACAAGGTCGGAACG+AGG 0.657316 4.2:-12596188 None:intergenic
CAATAACAAGGTCGGAACGA+GGG 0.670427 4.2:-12596187 None:intergenic
TGAGCACCTCCTTCATCAGG+AGG 0.676610 4.2:+12595832 MS.gene67232:CDS
CTAACAGCATTCCCTAACGT+GGG 0.718396 4.2:+12595873 MS.gene67232:CDS
TGTTATTGAGACACACAAGG+AGG 0.740119 4.2:+12596202 MS.gene67232:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGATGATCAATTAAGTGCT+GGG + chr4.2:12596141-12596160 MS.gene67232:CDS 30.0%
CACTTAATTGATCATCATCA+AGG - chr4.2:12596139-12596158 None:intergenic 30.0%
! TAAGTTCTTTAGTTCCATCT+GGG - chr4.2:12595977-12595996 None:intergenic 30.0%
GATGATGATCAATTAAGTGC+TGG + chr4.2:12596140-12596159 MS.gene67232:CDS 35.0%
TCGAGAATGTACTCATTATC+CGG - chr4.2:12596005-12596024 None:intergenic 35.0%
! AAGTTCTTTAGTTCCATCTG+GGG - chr4.2:12595976-12595995 None:intergenic 35.0%
! GTAAGTTCTTTAGTTCCATC+TGG - chr4.2:12595978-12595997 None:intergenic 35.0%
!! TCAGACTTTATTTGGTGTGA+AGG + chr4.2:12595896-12595915 MS.gene67232:CDS 35.0%
AAAAGTTGTAGAAGGGGAAG+TGG + chr4.2:12596094-12596113 MS.gene67232:CDS 40.0%
AACGTGGGTCAGACTTTATT+TGG + chr4.2:12595888-12595907 MS.gene67232:CDS 40.0%
AACTAAAGAACTTACCTGCC+CGG + chr4.2:12595983-12596002 MS.gene67232:CDS 40.0%
AATAAAGTCTGACCCACGTT+AGG - chr4.2:12595888-12595907 None:intergenic 40.0%
ATAAAGTCTGACCCACGTTA+GGG - chr4.2:12595887-12595906 None:intergenic 40.0%
CCTTGTGTGTCTCAATAACA+AGG - chr4.2:12596202-12596221 None:intergenic 40.0%
CGAGAATGTACTCATTATCC+GGG - chr4.2:12596004-12596023 None:intergenic 40.0%
GATGAGCTTGATCTTGTAAC+TGG - chr4.2:12595952-12595971 None:intergenic 40.0%
GTGCTGGAAAAGTTGTAGAA+GGG + chr4.2:12596087-12596106 MS.gene67232:CDS 40.0%
GTGTGTCTCAATAACAAGGT+CGG - chr4.2:12596198-12596217 None:intergenic 40.0%
TGCTGGAAAAGTTGTAGAAG+GGG + chr4.2:12596088-12596107 MS.gene67232:CDS 40.0%
TGTGCTGGAAAAGTTGTAGA+AGG + chr4.2:12596086-12596105 MS.gene67232:CDS 40.0%
TGTTATTGAGACACACAAGG+AGG + chr4.2:12596202-12596221 MS.gene67232:CDS 40.0%
!! ACTTTATTTGGTGTGAAGGC+CGG + chr4.2:12595900-12595919 MS.gene67232:CDS 40.0%
!! CCTTGTTATTGAGACACACA+AGG + chr4.2:12596199-12596218 MS.gene67232:CDS 40.0%
AATGTACTCATTATCCGGGC+AGG - chr4.2:12596000-12596019 None:intergenic 45.0%
CAATAACAAGGTCGGAACGA+GGG - chr4.2:12596190-12596209 None:intergenic 45.0%
CACGTTAGGGAATGCTGTTA+GGG - chr4.2:12595874-12595893 None:intergenic 45.0%
CGATCTTCCTTACTCATGCA+GGG + chr4.2:12596046-12596065 MS.gene67232:CDS 45.0%
CTAACAGCATTCCCTAACGT+GGG + chr4.2:12595873-12595892 MS.gene67232:CDS 45.0%
CTTGATCTTGTAACTGGCCA+TGG - chr4.2:12595946-12595965 None:intergenic 45.0%
GCTGTTAGGGTTGTGTTCAT+TGG - chr4.2:12595861-12595880 None:intergenic 45.0%
TCAATAACAAGGTCGGAACG+AGG - chr4.2:12596191-12596210 None:intergenic 45.0%
TCGATCTTCCTTACTCATGC+AGG + chr4.2:12596045-12596064 MS.gene67232:CDS 45.0%
! AAGATCGATGCCTTGTTCCT+CGG - chr4.2:12596033-12596052 None:intergenic 45.0%
AGTGATACGACTTCCACGTC+CGG - chr4.2:12595922-12595941 None:intergenic 50.0%
ATCAAGCTCATCACCCCAGA+TGG + chr4.2:12595960-12595979 MS.gene67232:CDS 50.0%
CCACGTTAGGGAATGCTGTT+AGG - chr4.2:12595875-12595894 None:intergenic 50.0%
CCTAACAGCATTCCCTAACG+TGG + chr4.2:12595872-12595891 MS.gene67232:CDS 50.0%
GCTCACCATGGTTCCTGATA+GGG - chr4.2:12595817-12595836 None:intergenic 50.0%
TGCTCACCATGGTTCCTGAT+AGG - chr4.2:12595818-12595837 None:intergenic 50.0%
TGGAAGTCGTATCACTGCCA+TGG + chr4.2:12595926-12595945 MS.gene67232:CDS 50.0%
AGCAGCCCTATCAGGAACCA+TGG + chr4.2:12595809-12595828 MS.gene67232:CDS 55.0%
CTTCCTTACTCATGCAGGGC+TGG + chr4.2:12596050-12596069 MS.gene67232:CDS 55.0%
GGTGCTTGCTCTTCATGTGC+TGG + chr4.2:12596071-12596090 MS.gene67232:CDS 55.0%
GTACATTCTCGACGTAGCCG+AGG + chr4.2:12596013-12596032 MS.gene67232:CDS 55.0%
TGAGCACCTCCTTCATCAGG+AGG + chr4.2:12595832-12595851 MS.gene67232:CDS 55.0%
TGGTGAGCACCTCCTTCATC+AGG + chr4.2:12595829-12595848 MS.gene67232:CDS 55.0%
! CATTGGCTGCCTCCTGATGA+AGG - chr4.2:12595844-12595863 None:intergenic 55.0%
!! GATGAAGGAGGTGCTCACCA+TGG - chr4.2:12595829-12595848 None:intergenic 55.0%
ACCACCACAGCAGCCCTATC+AGG + chr4.2:12595801-12595820 MS.gene67232:CDS 60.0%
CTCGACGTAGCCGAGGAACA+AGG + chr4.2:12596020-12596039 MS.gene67232:CDS 60.0%
GCACCAGCCCTGCATGAGTA+AGG - chr4.2:12596056-12596075 None:intergenic 60.0%
GGTTCCTGATAGGGCTGCTG+TGG - chr4.2:12595808-12595827 None:intergenic 60.0%
TCCTGATAGGGCTGCTGTGG+TGG - chr4.2:12595805-12595824 None:intergenic 60.0%
TGATAGGGCTGCTGTGGTGG+TGG - chr4.2:12595802-12595821 None:intergenic 60.0%
TGGCTGCCTCCTGATGAAGG+AGG - chr4.2:12595841-12595860 None:intergenic 60.0%
! TTTGGTGTGAAGGCCGGACG+TGG + chr4.2:12595906-12595925 MS.gene67232:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 12595795 12596241 12595795 ID=MS.gene67232
chr4.2 mRNA 12595795 12596241 12595795 ID=MS.gene67232.t1;Parent=MS.gene67232
chr4.2 exon 12595795 12596241 12595795 ID=MS.gene67232.t1.exon1;Parent=MS.gene67232.t1
chr4.2 CDS 12595795 12596241 12595795 ID=cds.MS.gene67232.t1;Parent=MS.gene67232.t1
Gene Sequence

>MS.gene67232

ATGGCCACCACCACAGCAGCCCTATCAGGAACCATGGTGAGCACCTCCTTCATCAGGAGGCAGCCAATGAACACAACCCTAACAGCATTCCCTAACGTGGGTCAGACTTTATTTGGTGTGAAGGCCGGACGTGGAAGTCGTATCACTGCCATGGCCAGTTACAAGATCAAGCTCATCACCCCAGATGGAACTAAAGAACTTACCTGCCCGGATAATGAGTACATTCTCGACGTAGCCGAGGAACAAGGCATCGATCTTCCTTACTCATGCAGGGCTGGTGCTTGCTCTTCATGTGCTGGAAAAGTTGTAGAAGGGGAAGTGGATAATTCAGATAATAGCTTCCTTGATGATGATCAATTAAGTGCTGGGTTTGTTCTCACATGTGTTGCTTACCCTCGTTCCGACCTTGTTATTGAGACACACAAGGAGGAAGATCTTGTTTCTTAA

Protein sequence

>MS.gene67232.t1

MATTTAALSGTMVSTSFIRRQPMNTTLTAFPNVGQTLFGVKAGRGSRITAMASYKIKLITPDGTKELTCPDNEYILDVAEEQGIDLPYSCRAGACSSCAGKVVEGEVDNSDNSFLDDDQLSAGFVLTCVAYPRSDLVIETHKEEDLVS