AlfalfaGEDB | MS.gene67232

M. sativa cultivar XinJiangDaYe / MS.gene67232

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67232.t1	XP_003629958.1	97.3	148	4	0	1	148	1	148	2.70E-75	291.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67232.t1	Q43517	67.1	143	47	0	6	148	2	144	3.6e-52	205.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67232.t1	G7L8U4	97.3	148	4	0	1	148	1	148	2.0e-75	291.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene67232	0.911449	6.01E-83	-1.69E-46
MS.gene049173	MS.gene67232	0.866708	2.34E-65	-1.69E-46
MS.gene049181	MS.gene67232	-0.811188	7.93E-51	-1.69E-46
MS.gene049217	MS.gene67232	0.871898	4.81E-67	-1.69E-46
MS.gene049222	MS.gene67232	0.879478	1.21E-69	-1.69E-46
MS.gene049232	MS.gene67232	0.895561	8.53E-76	-1.69E-46
MS.gene049281	MS.gene67232	-0.815118	1.09E-51	-1.69E-46
MS.gene049285	MS.gene67232	0.941161	6.72E-101	-1.69E-46
MS.gene049312	MS.gene67232	-0.814446	1.53E-51	-1.69E-46
MS.gene049313	MS.gene67232	-0.814437	1.54E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene67232	MS.gene74977	PPI
MS.gene67232	MS.gene000152	PPI
MS.gene67232	MS.gene067740	PPI
MS.gene67232	MS.gene034453	PPI
MS.gene67232	MS.gene92294	PPI
MS.gene67232	MS.gene03847	PPI
MS.gene67232	MS.gene029328	PPI
MS.gene67232	MS.gene88235	PPI
MS.gene67232	MS.gene065061	PPI
MS.gene67232	MS.gene35417	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67232.t1	MTR_8g088720	97.297	148	4	0	1	148	1	148	7.18e-107	301
MS.gene67232.t1	MTR_2g006290	63.816	152	44	3	4	148	3	150	1.87e-63	191
MS.gene67232.t1	MTR_1g112030	56.164	146	57	5	4	146	18	159	1.57e-50	159
MS.gene67232.t1	MTR_1g112030	56.164	146	57	5	4	146	14	155	1.65e-50	159
MS.gene67232.t1	MTR_1g112050	55.782	147	62	3	1	146	9	153	6.20e-50	157
MS.gene67232.t1	MTR_2g086510	61.789	123	45	2	25	146	33	154	1.05e-49	156
MS.gene67232.t1	MTR_4g053120	57.037	135	51	3	19	146	20	154	5.77e-48	152
MS.gene67232.t1	MTR_5g006320	36.634	101	63	1	48	148	46	145	1.67e-17	74.7
MS.gene67232.t1	MTR_4g098490	36.585	82	52	0	65	146	77	158	3.29e-16	72.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene67232.t1	AT1G60950	64.000	150	49	3	1	147	1	148	1.03e-65	197
MS.gene67232.t1	AT1G10960	61.333	150	53	3	1	147	1	148	5.54e-59	180
MS.gene67232.t1	AT2G27510	50.658	152	65	4	5	146	3	154	1.99e-44	143
MS.gene67232.t1	AT5G10000	43.182	132	71	3	19	147	18	148	1.23e-32	113
MS.gene67232.t1	AT4G14890	35.333	150	80	4	3	148	10	146	7.54e-17	72.8
MS.gene67232.t1	AT1G32550	40.244	82	49	0	65	146	72	153	1.26e-16	73.2
MS.gene67232.t1	AT1G32550	34.783	92	44	2	71	146	75	166	5.22e-12	60.8

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Find 56 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTGTAGAAGGGGAAG+TGG	0.534366	4.2:+12596094	MS.gene67232:CDS
AACGTGGGTCAGACTTTATT+TGG	0.300570	4.2:+12595888	MS.gene67232:CDS
AACTAAAGAACTTACCTGCC+CGG	0.559896	4.2:+12595983	MS.gene67232:CDS
AAGATCGATGCCTTGTTCCT+CGG	0.553403	4.2:-12596030	None:intergenic
AAGTTCTTTAGTTCCATCTG+GGG	0.632400	4.2:-12595973	None:intergenic
AATAAAGTCTGACCCACGTT+AGG	0.420347	4.2:-12595885	None:intergenic
AATGTACTCATTATCCGGGC+AGG	0.566913	4.2:-12595997	None:intergenic
ACCACCACAGCAGCCCTATC+AGG	0.384793	4.2:+12595801	MS.gene67232:CDS
ACTTTATTTGGTGTGAAGGC+CGG	0.475879	4.2:+12595900	MS.gene67232:CDS
AGCAGCCCTATCAGGAACCA+TGG	0.637647	4.2:+12595809	MS.gene67232:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGATGATCAATTAAGTGCT+GGG	+	chr4.2:12596141-12596160	MS.gene67232:CDS	30.0%
CACTTAATTGATCATCATCA+AGG	-	chr4.2:12596139-12596158	None:intergenic	30.0%
GATGATGATCAATTAAGTGC+TGG	+	chr4.2:12596140-12596159	MS.gene67232:CDS	35.0%
TCGAGAATGTACTCATTATC+CGG	-	chr4.2:12596005-12596024	None:intergenic	35.0%
AAAAGTTGTAGAAGGGGAAG+TGG	+	chr4.2:12596094-12596113	MS.gene67232:CDS	40.0%
AACGTGGGTCAGACTTTATT+TGG	+	chr4.2:12595888-12595907	MS.gene67232:CDS	40.0%
AACTAAAGAACTTACCTGCC+CGG	+	chr4.2:12595983-12596002	MS.gene67232:CDS	40.0%
AATAAAGTCTGACCCACGTT+AGG	-	chr4.2:12595888-12595907	None:intergenic	40.0%
ATAAAGTCTGACCCACGTTA+GGG	-	chr4.2:12595887-12595906	None:intergenic	40.0%
CCTTGTGTGTCTCAATAACA+AGG	-	chr4.2:12596202-12596221	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	12595795	12596241	12595795	ID=MS.gene67232
chr4.2	mRNA	12595795	12596241	12595795	ID=MS.gene67232.t1;Parent=MS.gene67232
chr4.2	exon	12595795	12596241	12595795	ID=MS.gene67232.t1.exon1;Parent=MS.gene67232.t1
chr4.2	CDS	12595795	12596241	12595795	ID=cds.MS.gene67232.t1;Parent=MS.gene67232.t1

Gene Sequence

>MS.gene67232

ATGGCCACCACCACAGCAGCCCTATCAGGAACCATGGTGAGCACCTCCTTCATCAGGAGGCAGCCAATGAACACAACCCTAACAGCATTCCCTAACGTGGGTCAGACTTTATTTGGTGTGAAGGCCGGACGTGGAAGTCGTATCACTGCCATGGCCAGTTACAAGATCAAGCTCATCACCCCAGATGGAACTAAAGAACTTACCTGCCCGGATAATGAGTACATTCTCGACGTAGCCGAGGAACAAGGCATCGATCTTCCTTACTCATGCAGGGCTGGTGCTTGCTCTTCATGTGCTGGAAAAGTTGTAGAAGGGGAAGTGGATAATTCAGATAATAGCTTCCTTGATGATGATCAATTAAGTGCTGGGTTTGTTCTCACATGTGTTGCTTACCCTCGTTCCGACCTTGTTATTGAGACACACAAGGAGGAAGATCTTGTTTCTTAA

Protein sequence

>MS.gene67232.t1

MATTTAALSGTMVSTSFIRRQPMNTTLTAFPNVGQTLFGVKAGRGSRITAMASYKIKLITPDGTKELTCPDNEYILDVAEEQGIDLPYSCRAGACSSCAGKVVEGEVDNSDNSFLDDDQLSAGFVLTCVAYPRSDLVIETHKEEDLVS