AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene47889


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47889.t1 MTR_8g088720 97.297 148 4 0 1 148 1 148 7.18e-107 301
MS.gene47889.t1 MTR_2g006290 63.816 152 44 3 4 148 3 150 1.87e-63 191
MS.gene47889.t1 MTR_1g112030 56.164 146 57 5 4 146 18 159 1.57e-50 159
MS.gene47889.t1 MTR_1g112030 56.164 146 57 5 4 146 14 155 1.65e-50 159
MS.gene47889.t1 MTR_1g112050 55.782 147 62 3 1 146 9 153 6.20e-50 157
MS.gene47889.t1 MTR_2g086510 61.789 123 45 2 25 146 33 154 1.05e-49 156
MS.gene47889.t1 MTR_4g053120 57.037 135 51 3 19 146 20 154 5.77e-48 152
MS.gene47889.t1 MTR_5g006320 36.634 101 63 1 48 148 46 145 1.67e-17 74.7
MS.gene47889.t1 MTR_4g098490 36.585 82 52 0 65 146 77 158 3.29e-16 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene47889.t1 AT1G60950 64.000 150 49 3 1 147 1 148 1.03e-65 197
MS.gene47889.t1 AT1G10960 61.333 150 53 3 1 147 1 148 5.54e-59 180
MS.gene47889.t1 AT2G27510 50.658 152 65 4 5 146 3 154 1.99e-44 143
MS.gene47889.t1 AT5G10000 43.182 132 71 3 19 147 18 148 1.23e-32 113
MS.gene47889.t1 AT4G14890 35.333 150 80 4 3 148 10 146 7.54e-17 72.8
MS.gene47889.t1 AT1G32550 40.244 82 49 0 65 146 72 153 1.26e-16 73.2
MS.gene47889.t1 AT1G32550 34.783 92 44 2 71 146 75 166 5.22e-12 60.8

Find 56 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGTGGGTCAGACTTTATT+TGG 0.300570 4.1:+11884472 MS.gene47889:CDS
CCACGTTAGGGAATGCTGTT+AGG 0.301806 4.1:-11884456 None:intergenic
GTAAGTTCTTTAGTTCCATC+TGG 0.311839 4.1:-11884559 None:intergenic
GCTGTTAGGGTTGTGTTCAT+TGG 0.358114 4.1:-11884442 None:intergenic
ACCACCACAGCAGCCCTATC+AGG 0.384793 4.1:+11884385 MS.gene47889:CDS
TGCTCACCATGGTTCCTGAT+AGG 0.397541 4.1:-11884399 None:intergenic
TGTGCTGGAAAAGTTGTAGA+AGG 0.411785 4.1:+11884670 MS.gene47889:CDS
AATAAAGTCTGACCCACGTT+AGG 0.420347 4.1:-11884469 None:intergenic
TCGAGAATGTACTCATTATC+CGG 0.426007 4.1:-11884586 None:intergenic
GATGAGCTTGATCTTGTAAC+TGG 0.426566 4.1:-11884533 None:intergenic
CTTCCTTACTCATGCAGGGC+TGG 0.437541 4.1:+11884634 MS.gene47889:CDS
GGTGCTTGCTCTTCATGTGC+TGG 0.439901 4.1:+11884655 MS.gene47889:CDS
TCGATCTTCCTTACTCATGC+AGG 0.452165 4.1:+11884629 MS.gene47889:CDS
TAAGTTCTTTAGTTCCATCT+GGG 0.455484 4.1:-11884558 None:intergenic
GCTCACCATGGTTCCTGATA+GGG 0.466904 4.1:-11884398 None:intergenic
TCCTGATAGGGCTGCTGTGG+TGG 0.470793 4.1:-11884386 None:intergenic
CATTGGCTGCCTCCTGATGA+AGG 0.472321 4.1:-11884425 None:intergenic
ACTTTATTTGGTGTGAAGGC+CGG 0.475879 4.1:+11884484 MS.gene47889:CDS
TGATAGGGCTGCTGTGGTGG+TGG 0.476979 4.1:-11884383 None:intergenic
TGGTGAGCACCTCCTTCATC+AGG 0.483290 4.1:+11884413 MS.gene47889:CDS
CACGTTAGGGAATGCTGTTA+GGG 0.501804 4.1:-11884455 None:intergenic
GCACCAGCCCTGCATGAGTA+AGG 0.502602 4.1:-11884637 None:intergenic
GATGATGATCAATTAAGTGC+TGG 0.507761 4.1:+11884724 MS.gene47889:CDS
TCAGACTTTATTTGGTGTGA+AGG 0.514081 4.1:+11884480 MS.gene47889:CDS
ATAAAGTCTGACCCACGTTA+GGG 0.523812 4.1:-11884468 None:intergenic
AAAAGTTGTAGAAGGGGAAG+TGG 0.534366 4.1:+11884678 MS.gene47889:CDS
ATCAAGCTCATCACCCCAGA+TGG 0.540257 4.1:+11884544 MS.gene47889:CDS
CTTGATCTTGTAACTGGCCA+TGG 0.544201 4.1:-11884527 None:intergenic
ATGATGATCAATTAAGTGCT+GGG 0.551795 4.1:+11884725 MS.gene47889:CDS
AAGATCGATGCCTTGTTCCT+CGG 0.553403 4.1:-11884614 None:intergenic
CTCGACGTAGCCGAGGAACA+AGG 0.555180 4.1:+11884604 MS.gene47889:CDS
CACTTAATTGATCATCATCA+AGG 0.556300 4.1:-11884720 None:intergenic
AACTAAAGAACTTACCTGCC+CGG 0.559896 4.1:+11884567 MS.gene47889:CDS
TTTGGTGTGAAGGCCGGACG+TGG 0.560810 4.1:+11884490 MS.gene47889:CDS
AATGTACTCATTATCCGGGC+AGG 0.566913 4.1:-11884581 None:intergenic
CGATCTTCCTTACTCATGCA+GGG 0.578511 4.1:+11884630 MS.gene47889:CDS
GTGCTGGAAAAGTTGTAGAA+GGG 0.579438 4.1:+11884671 MS.gene47889:CDS
GGTTCCTGATAGGGCTGCTG+TGG 0.579627 4.1:-11884389 None:intergenic
AGTGATACGACTTCCACGTC+CGG 0.584494 4.1:-11884503 None:intergenic
CGAGAATGTACTCATTATCC+GGG 0.584894 4.1:-11884585 None:intergenic
CCTTGTGTGTCTCAATAACA+AGG 0.591064 4.1:-11884783 None:intergenic
CCTAACAGCATTCCCTAACG+TGG 0.597665 4.1:+11884456 MS.gene47889:CDS
TGCTGGAAAAGTTGTAGAAG+GGG 0.598351 4.1:+11884672 MS.gene47889:CDS
TGGAAGTCGTATCACTGCCA+TGG 0.614707 4.1:+11884510 MS.gene47889:CDS
GTACATTCTCGACGTAGCCG+AGG 0.630338 4.1:+11884597 MS.gene47889:CDS
AAGTTCTTTAGTTCCATCTG+GGG 0.632400 4.1:-11884557 None:intergenic
AGCAGCCCTATCAGGAACCA+TGG 0.637647 4.1:+11884393 MS.gene47889:CDS
TGGCTGCCTCCTGATGAAGG+AGG 0.644050 4.1:-11884422 None:intergenic
GTGTGTCTCAATAACAAGGT+CGG 0.646026 4.1:-11884779 None:intergenic
CCTTGTTATTGAGACACACA+AGG 0.649100 4.1:+11884783 MS.gene47889:CDS
GATGAAGGAGGTGCTCACCA+TGG 0.650251 4.1:-11884410 None:intergenic
TCAATAACAAGGTCGGAACG+AGG 0.657316 4.1:-11884772 None:intergenic
CAATAACAAGGTCGGAACGA+GGG 0.670427 4.1:-11884771 None:intergenic
TGAGCACCTCCTTCATCAGG+AGG 0.676610 4.1:+11884416 MS.gene47889:CDS
CTAACAGCATTCCCTAACGT+GGG 0.718396 4.1:+11884457 MS.gene47889:CDS
TGTTATTGAGACACACAAGG+AGG 0.740119 4.1:+11884786 MS.gene47889:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGATGATCAATTAAGTGCT+GGG + chr4.1:11884725-11884744 MS.gene47889:CDS 30.0%
CACTTAATTGATCATCATCA+AGG - chr4.1:11884723-11884742 None:intergenic 30.0%
! TAAGTTCTTTAGTTCCATCT+GGG - chr4.1:11884561-11884580 None:intergenic 30.0%
GATGATGATCAATTAAGTGC+TGG + chr4.1:11884724-11884743 MS.gene47889:CDS 35.0%
TCGAGAATGTACTCATTATC+CGG - chr4.1:11884589-11884608 None:intergenic 35.0%
! AAGTTCTTTAGTTCCATCTG+GGG - chr4.1:11884560-11884579 None:intergenic 35.0%
! GTAAGTTCTTTAGTTCCATC+TGG - chr4.1:11884562-11884581 None:intergenic 35.0%
!! TCAGACTTTATTTGGTGTGA+AGG + chr4.1:11884480-11884499 MS.gene47889:CDS 35.0%
AAAAGTTGTAGAAGGGGAAG+TGG + chr4.1:11884678-11884697 MS.gene47889:CDS 40.0%
AACGTGGGTCAGACTTTATT+TGG + chr4.1:11884472-11884491 MS.gene47889:CDS 40.0%
AACTAAAGAACTTACCTGCC+CGG + chr4.1:11884567-11884586 MS.gene47889:CDS 40.0%
AATAAAGTCTGACCCACGTT+AGG - chr4.1:11884472-11884491 None:intergenic 40.0%
ATAAAGTCTGACCCACGTTA+GGG - chr4.1:11884471-11884490 None:intergenic 40.0%
CCTTGTGTGTCTCAATAACA+AGG - chr4.1:11884786-11884805 None:intergenic 40.0%
CGAGAATGTACTCATTATCC+GGG - chr4.1:11884588-11884607 None:intergenic 40.0%
GATGAGCTTGATCTTGTAAC+TGG - chr4.1:11884536-11884555 None:intergenic 40.0%
GTGCTGGAAAAGTTGTAGAA+GGG + chr4.1:11884671-11884690 MS.gene47889:CDS 40.0%
GTGTGTCTCAATAACAAGGT+CGG - chr4.1:11884782-11884801 None:intergenic 40.0%
TGCTGGAAAAGTTGTAGAAG+GGG + chr4.1:11884672-11884691 MS.gene47889:CDS 40.0%
TGTGCTGGAAAAGTTGTAGA+AGG + chr4.1:11884670-11884689 MS.gene47889:CDS 40.0%
TGTTATTGAGACACACAAGG+AGG + chr4.1:11884786-11884805 MS.gene47889:CDS 40.0%
!! ACTTTATTTGGTGTGAAGGC+CGG + chr4.1:11884484-11884503 MS.gene47889:CDS 40.0%
!! CCTTGTTATTGAGACACACA+AGG + chr4.1:11884783-11884802 MS.gene47889:CDS 40.0%
AATGTACTCATTATCCGGGC+AGG - chr4.1:11884584-11884603 None:intergenic 45.0%
CAATAACAAGGTCGGAACGA+GGG - chr4.1:11884774-11884793 None:intergenic 45.0%
CACGTTAGGGAATGCTGTTA+GGG - chr4.1:11884458-11884477 None:intergenic 45.0%
CGATCTTCCTTACTCATGCA+GGG + chr4.1:11884630-11884649 MS.gene47889:CDS 45.0%
CTAACAGCATTCCCTAACGT+GGG + chr4.1:11884457-11884476 MS.gene47889:CDS 45.0%
CTTGATCTTGTAACTGGCCA+TGG - chr4.1:11884530-11884549 None:intergenic 45.0%
GCTGTTAGGGTTGTGTTCAT+TGG - chr4.1:11884445-11884464 None:intergenic 45.0%
TCAATAACAAGGTCGGAACG+AGG - chr4.1:11884775-11884794 None:intergenic 45.0%
TCGATCTTCCTTACTCATGC+AGG + chr4.1:11884629-11884648 MS.gene47889:CDS 45.0%
! AAGATCGATGCCTTGTTCCT+CGG - chr4.1:11884617-11884636 None:intergenic 45.0%
AGTGATACGACTTCCACGTC+CGG - chr4.1:11884506-11884525 None:intergenic 50.0%
ATCAAGCTCATCACCCCAGA+TGG + chr4.1:11884544-11884563 MS.gene47889:CDS 50.0%
CCACGTTAGGGAATGCTGTT+AGG - chr4.1:11884459-11884478 None:intergenic 50.0%
CCTAACAGCATTCCCTAACG+TGG + chr4.1:11884456-11884475 MS.gene47889:CDS 50.0%
GCTCACCATGGTTCCTGATA+GGG - chr4.1:11884401-11884420 None:intergenic 50.0%
TGCTCACCATGGTTCCTGAT+AGG - chr4.1:11884402-11884421 None:intergenic 50.0%
TGGAAGTCGTATCACTGCCA+TGG + chr4.1:11884510-11884529 MS.gene47889:CDS 50.0%
AGCAGCCCTATCAGGAACCA+TGG + chr4.1:11884393-11884412 MS.gene47889:CDS 55.0%
CTTCCTTACTCATGCAGGGC+TGG + chr4.1:11884634-11884653 MS.gene47889:CDS 55.0%
GGTGCTTGCTCTTCATGTGC+TGG + chr4.1:11884655-11884674 MS.gene47889:CDS 55.0%
GTACATTCTCGACGTAGCCG+AGG + chr4.1:11884597-11884616 MS.gene47889:CDS 55.0%
TGAGCACCTCCTTCATCAGG+AGG + chr4.1:11884416-11884435 MS.gene47889:CDS 55.0%
TGGTGAGCACCTCCTTCATC+AGG + chr4.1:11884413-11884432 MS.gene47889:CDS 55.0%
! CATTGGCTGCCTCCTGATGA+AGG - chr4.1:11884428-11884447 None:intergenic 55.0%
!! GATGAAGGAGGTGCTCACCA+TGG - chr4.1:11884413-11884432 None:intergenic 55.0%
ACCACCACAGCAGCCCTATC+AGG + chr4.1:11884385-11884404 MS.gene47889:CDS 60.0%
CTCGACGTAGCCGAGGAACA+AGG + chr4.1:11884604-11884623 MS.gene47889:CDS 60.0%
GCACCAGCCCTGCATGAGTA+AGG - chr4.1:11884640-11884659 None:intergenic 60.0%
GGTTCCTGATAGGGCTGCTG+TGG - chr4.1:11884392-11884411 None:intergenic 60.0%
TCCTGATAGGGCTGCTGTGG+TGG - chr4.1:11884389-11884408 None:intergenic 60.0%
TGATAGGGCTGCTGTGGTGG+TGG - chr4.1:11884386-11884405 None:intergenic 60.0%
TGGCTGCCTCCTGATGAAGG+AGG - chr4.1:11884425-11884444 None:intergenic 60.0%
! TTTGGTGTGAAGGCCGGACG+TGG + chr4.1:11884490-11884509 MS.gene47889:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 11884379 11884825 11884379 ID=MS.gene47889
chr4.1 mRNA 11884379 11884825 11884379 ID=MS.gene47889.t1;Parent=MS.gene47889
chr4.1 exon 11884379 11884825 11884379 ID=MS.gene47889.t1.exon1;Parent=MS.gene47889.t1
chr4.1 CDS 11884379 11884825 11884379 ID=cds.MS.gene47889.t1;Parent=MS.gene47889.t1
Gene Sequence

>MS.gene47889

ATGGCCACCACCACAGCAGCCCTATCAGGAACCATGGTGAGCACCTCCTTCATCAGGAGGCAGCCAATGAACACAACCCTAACAGCATTCCCTAACGTGGGTCAGACTTTATTTGGTGTGAAGGCCGGACGTGGAAGTCGTATCACTGCCATGGCCAGTTACAAGATCAAGCTCATCACCCCAGATGGAACTAAAGAACTTACCTGCCCGGATAATGAGTACATTCTCGACGTAGCCGAGGAACAAGGCATCGATCTTCCTTACTCATGCAGGGCTGGTGCTTGCTCTTCATGTGCTGGAAAAGTTGTAGAAGGGGAAGTGGATAATTCAGATAATAGCTTCCTTGATGATGATCAATTAAGTGCTGGGTTTGTTCTCACATGTGTTGCTTACCCTCGTTCCGACCTTGTTATTGAGACACACAAGGAGGAAGATCTTGTTTCTTAA

Protein sequence

>MS.gene47889.t1

MATTTAALSGTMVSTSFIRRQPMNTTLTAFPNVGQTLFGVKAGRGSRITAMASYKIKLITPDGTKELTCPDNEYILDVAEEQGIDLPYSCRAGACSSCAGKVVEGEVDNSDNSFLDDDQLSAGFVLTCVAYPRSDLVIETHKEEDLVS