AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27932


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27932.t1 MTR_8g088720 97.297 148 4 0 1 148 1 148 7.18e-107 301
MS.gene27932.t1 MTR_2g006290 63.816 152 44 3 4 148 3 150 1.87e-63 191
MS.gene27932.t1 MTR_1g112030 56.164 146 57 5 4 146 18 159 1.57e-50 159
MS.gene27932.t1 MTR_1g112030 56.164 146 57 5 4 146 14 155 1.65e-50 159
MS.gene27932.t1 MTR_1g112050 55.782 147 62 3 1 146 9 153 6.20e-50 157
MS.gene27932.t1 MTR_2g086510 61.789 123 45 2 25 146 33 154 1.05e-49 156
MS.gene27932.t1 MTR_4g053120 57.037 135 51 3 19 146 20 154 5.77e-48 152
MS.gene27932.t1 MTR_5g006320 36.634 101 63 1 48 148 46 145 1.67e-17 74.7
MS.gene27932.t1 MTR_4g098490 36.585 82 52 0 65 146 77 158 3.29e-16 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27932.t1 AT1G60950 64.000 150 49 3 1 147 1 148 1.03e-65 197
MS.gene27932.t1 AT1G10960 61.333 150 53 3 1 147 1 148 5.54e-59 180
MS.gene27932.t1 AT2G27510 50.658 152 65 4 5 146 3 154 1.99e-44 143
MS.gene27932.t1 AT5G10000 43.182 132 71 3 19 147 18 148 1.23e-32 113
MS.gene27932.t1 AT4G14890 35.333 150 80 4 3 148 10 146 7.54e-17 72.8
MS.gene27932.t1 AT1G32550 40.244 82 49 0 65 146 72 153 1.26e-16 73.2
MS.gene27932.t1 AT1G32550 34.783 92 44 2 71 146 75 166 5.22e-12 60.8

Find 56 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACGTGGGTCAGACTTTATT+TGG 0.300570 4.4:+12313960 MS.gene27932:CDS
CCACGTTAGGGAATGCTGTT+AGG 0.301806 4.4:-12313944 None:intergenic
GTAAGTTCTTTAGTTCCATC+TGG 0.311839 4.4:-12314047 None:intergenic
GCTGTTAGGGTTGTGTTCAT+TGG 0.358114 4.4:-12313930 None:intergenic
ACCACCACAGCAGCCCTATC+AGG 0.384793 4.4:+12313873 MS.gene27932:CDS
TGCTCACCATGGTTCCTGAT+AGG 0.397541 4.4:-12313887 None:intergenic
TGTGCTGGAAAAGTTGTAGA+AGG 0.411785 4.4:+12314158 MS.gene27932:CDS
AATAAAGTCTGACCCACGTT+AGG 0.420347 4.4:-12313957 None:intergenic
AATGAGCTTGATCTTGTAAC+TGG 0.424868 4.4:-12314021 None:intergenic
TCGAGAATGTACTCATTATC+CGG 0.426007 4.4:-12314074 None:intergenic
CTTCCTTACTCATGCAGGGC+TGG 0.437541 4.4:+12314122 MS.gene27932:CDS
GGTGCTTGCTCTTCATGTGC+TGG 0.439901 4.4:+12314143 MS.gene27932:CDS
TCGATCTTCCTTACTCATGC+AGG 0.452165 4.4:+12314117 MS.gene27932:CDS
GCTCACCATGGTTCCTGATA+GGG 0.466904 4.4:-12313886 None:intergenic
TCCTGATAGGGCTGCTGTGG+TGG 0.470793 4.4:-12313874 None:intergenic
CATTGGCTGCCTCCTGATGA+AGG 0.472321 4.4:-12313913 None:intergenic
ACTTTATTTGGTGTGAAGGC+CGG 0.475879 4.4:+12313972 MS.gene27932:CDS
TGATAGGGCTGCTGTGGTGG+TGG 0.476979 4.4:-12313871 None:intergenic
TGGTGAGCACCTCCTTCATC+AGG 0.483290 4.4:+12313901 MS.gene27932:CDS
TAAGTTCTTTAGTTCCATCT+GGG 0.497202 4.4:-12314046 None:intergenic
CACGTTAGGGAATGCTGTTA+GGG 0.501804 4.4:-12313943 None:intergenic
GCACCAGCCCTGCATGAGTA+AGG 0.502602 4.4:-12314125 None:intergenic
GATGATGATCAATTAAGTGC+TGG 0.507761 4.4:+12314212 MS.gene27932:CDS
TCAGACTTTATTTGGTGTGA+AGG 0.514081 4.4:+12313968 MS.gene27932:CDS
ATAAAGTCTGACCCACGTTA+GGG 0.523812 4.4:-12313956 None:intergenic
ATCAAGCTCATTACCCCAGA+TGG 0.528289 4.4:+12314032 MS.gene27932:CDS
AAAAGTTGTAGAAGGGGAAG+TGG 0.534366 4.4:+12314166 MS.gene27932:CDS
CTTGATCTTGTAACTGGCCA+TGG 0.544201 4.4:-12314015 None:intergenic
ATGATGATCAATTAAGTGCT+GGG 0.551795 4.4:+12314213 MS.gene27932:CDS
AAGATCGATGCCTTGTTCCT+CGG 0.553403 4.4:-12314102 None:intergenic
CTCGACGTAGCCGAGGAACA+AGG 0.555180 4.4:+12314092 MS.gene27932:CDS
CACTTAATTGATCATCATCA+AGG 0.556300 4.4:-12314208 None:intergenic
AACTAAAGAACTTACCTGCC+CGG 0.559896 4.4:+12314055 MS.gene27932:CDS
TTTGGTGTGAAGGCCGGACG+TGG 0.560810 4.4:+12313978 MS.gene27932:CDS
AATGTACTCATTATCCGGGC+AGG 0.566913 4.4:-12314069 None:intergenic
CGATCTTCCTTACTCATGCA+GGG 0.578511 4.4:+12314118 MS.gene27932:CDS
GTGCTGGAAAAGTTGTAGAA+GGG 0.579438 4.4:+12314159 MS.gene27932:CDS
GGTTCCTGATAGGGCTGCTG+TGG 0.579627 4.4:-12313877 None:intergenic
AGTGATACGACTTCCACGTC+CGG 0.584494 4.4:-12313991 None:intergenic
CGAGAATGTACTCATTATCC+GGG 0.584894 4.4:-12314073 None:intergenic
CCTTGTGTGTCTCAATAACA+AGG 0.591064 4.4:-12314271 None:intergenic
CCTAACAGCATTCCCTAACG+TGG 0.597665 4.4:+12313944 MS.gene27932:CDS
TGCTGGAAAAGTTGTAGAAG+GGG 0.598351 4.4:+12314160 MS.gene27932:CDS
TGGAAGTCGTATCACTGCCA+TGG 0.614707 4.4:+12313998 MS.gene27932:CDS
GTACATTCTCGACGTAGCCG+AGG 0.630338 4.4:+12314085 MS.gene27932:CDS
AAGTTCTTTAGTTCCATCTG+GGG 0.631501 4.4:-12314045 None:intergenic
AGCAGCCCTATCAGGAACCA+TGG 0.637647 4.4:+12313881 MS.gene27932:CDS
TGGCTGCCTCCTGATGAAGG+AGG 0.644050 4.4:-12313910 None:intergenic
GTGTGTCTCAATAACAAGGT+CGG 0.646026 4.4:-12314267 None:intergenic
CCTTGTTATTGAGACACACA+AGG 0.649100 4.4:+12314271 MS.gene27932:CDS
GATGAAGGAGGTGCTCACCA+TGG 0.650251 4.4:-12313898 None:intergenic
TCAATAACAAGGTCGGAACG+AGG 0.657316 4.4:-12314260 None:intergenic
CAATAACAAGGTCGGAACGA+GGG 0.670427 4.4:-12314259 None:intergenic
TGAGCACCTCCTTCATCAGG+AGG 0.676610 4.4:+12313904 MS.gene27932:CDS
CTAACAGCATTCCCTAACGT+GGG 0.718396 4.4:+12313945 MS.gene27932:CDS
TGTTATTGAGACACACAAGG+AGG 0.740119 4.4:+12314274 MS.gene27932:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGATGATCAATTAAGTGCT+GGG + chr4.4:12314213-12314232 MS.gene27932:CDS 30.0%
CACTTAATTGATCATCATCA+AGG - chr4.4:12314211-12314230 None:intergenic 30.0%
! TAAGTTCTTTAGTTCCATCT+GGG - chr4.4:12314049-12314068 None:intergenic 30.0%
AATGAGCTTGATCTTGTAAC+TGG - chr4.4:12314024-12314043 None:intergenic 35.0%
GATGATGATCAATTAAGTGC+TGG + chr4.4:12314212-12314231 MS.gene27932:CDS 35.0%
TCGAGAATGTACTCATTATC+CGG - chr4.4:12314077-12314096 None:intergenic 35.0%
! AAGTTCTTTAGTTCCATCTG+GGG - chr4.4:12314048-12314067 None:intergenic 35.0%
! GTAAGTTCTTTAGTTCCATC+TGG - chr4.4:12314050-12314069 None:intergenic 35.0%
!! TCAGACTTTATTTGGTGTGA+AGG + chr4.4:12313968-12313987 MS.gene27932:CDS 35.0%
AAAAGTTGTAGAAGGGGAAG+TGG + chr4.4:12314166-12314185 MS.gene27932:CDS 40.0%
AACGTGGGTCAGACTTTATT+TGG + chr4.4:12313960-12313979 MS.gene27932:CDS 40.0%
AACTAAAGAACTTACCTGCC+CGG + chr4.4:12314055-12314074 MS.gene27932:CDS 40.0%
AATAAAGTCTGACCCACGTT+AGG - chr4.4:12313960-12313979 None:intergenic 40.0%
ATAAAGTCTGACCCACGTTA+GGG - chr4.4:12313959-12313978 None:intergenic 40.0%
CCTTGTGTGTCTCAATAACA+AGG - chr4.4:12314274-12314293 None:intergenic 40.0%
CGAGAATGTACTCATTATCC+GGG - chr4.4:12314076-12314095 None:intergenic 40.0%
GTGCTGGAAAAGTTGTAGAA+GGG + chr4.4:12314159-12314178 MS.gene27932:CDS 40.0%
GTGTGTCTCAATAACAAGGT+CGG - chr4.4:12314270-12314289 None:intergenic 40.0%
TGCTGGAAAAGTTGTAGAAG+GGG + chr4.4:12314160-12314179 MS.gene27932:CDS 40.0%
TGTGCTGGAAAAGTTGTAGA+AGG + chr4.4:12314158-12314177 MS.gene27932:CDS 40.0%
TGTTATTGAGACACACAAGG+AGG + chr4.4:12314274-12314293 MS.gene27932:CDS 40.0%
!! ACTTTATTTGGTGTGAAGGC+CGG + chr4.4:12313972-12313991 MS.gene27932:CDS 40.0%
!! CCTTGTTATTGAGACACACA+AGG + chr4.4:12314271-12314290 MS.gene27932:CDS 40.0%
AATGTACTCATTATCCGGGC+AGG - chr4.4:12314072-12314091 None:intergenic 45.0%
ATCAAGCTCATTACCCCAGA+TGG + chr4.4:12314032-12314051 MS.gene27932:CDS 45.0%
CAATAACAAGGTCGGAACGA+GGG - chr4.4:12314262-12314281 None:intergenic 45.0%
CACGTTAGGGAATGCTGTTA+GGG - chr4.4:12313946-12313965 None:intergenic 45.0%
CGATCTTCCTTACTCATGCA+GGG + chr4.4:12314118-12314137 MS.gene27932:CDS 45.0%
CTAACAGCATTCCCTAACGT+GGG + chr4.4:12313945-12313964 MS.gene27932:CDS 45.0%
CTTGATCTTGTAACTGGCCA+TGG - chr4.4:12314018-12314037 None:intergenic 45.0%
GCTGTTAGGGTTGTGTTCAT+TGG - chr4.4:12313933-12313952 None:intergenic 45.0%
TCAATAACAAGGTCGGAACG+AGG - chr4.4:12314263-12314282 None:intergenic 45.0%
TCGATCTTCCTTACTCATGC+AGG + chr4.4:12314117-12314136 MS.gene27932:CDS 45.0%
! AAGATCGATGCCTTGTTCCT+CGG - chr4.4:12314105-12314124 None:intergenic 45.0%
AGTGATACGACTTCCACGTC+CGG - chr4.4:12313994-12314013 None:intergenic 50.0%
CCACGTTAGGGAATGCTGTT+AGG - chr4.4:12313947-12313966 None:intergenic 50.0%
CCTAACAGCATTCCCTAACG+TGG + chr4.4:12313944-12313963 MS.gene27932:CDS 50.0%
GCTCACCATGGTTCCTGATA+GGG - chr4.4:12313889-12313908 None:intergenic 50.0%
TGCTCACCATGGTTCCTGAT+AGG - chr4.4:12313890-12313909 None:intergenic 50.0%
TGGAAGTCGTATCACTGCCA+TGG + chr4.4:12313998-12314017 MS.gene27932:CDS 50.0%
AGCAGCCCTATCAGGAACCA+TGG + chr4.4:12313881-12313900 MS.gene27932:CDS 55.0%
CTTCCTTACTCATGCAGGGC+TGG + chr4.4:12314122-12314141 MS.gene27932:CDS 55.0%
GGTGCTTGCTCTTCATGTGC+TGG + chr4.4:12314143-12314162 MS.gene27932:CDS 55.0%
GTACATTCTCGACGTAGCCG+AGG + chr4.4:12314085-12314104 MS.gene27932:CDS 55.0%
TGAGCACCTCCTTCATCAGG+AGG + chr4.4:12313904-12313923 MS.gene27932:CDS 55.0%
TGGTGAGCACCTCCTTCATC+AGG + chr4.4:12313901-12313920 MS.gene27932:CDS 55.0%
! CATTGGCTGCCTCCTGATGA+AGG - chr4.4:12313916-12313935 None:intergenic 55.0%
!! GATGAAGGAGGTGCTCACCA+TGG - chr4.4:12313901-12313920 None:intergenic 55.0%
ACCACCACAGCAGCCCTATC+AGG + chr4.4:12313873-12313892 MS.gene27932:CDS 60.0%
CTCGACGTAGCCGAGGAACA+AGG + chr4.4:12314092-12314111 MS.gene27932:CDS 60.0%
GCACCAGCCCTGCATGAGTA+AGG - chr4.4:12314128-12314147 None:intergenic 60.0%
GGTTCCTGATAGGGCTGCTG+TGG - chr4.4:12313880-12313899 None:intergenic 60.0%
TCCTGATAGGGCTGCTGTGG+TGG - chr4.4:12313877-12313896 None:intergenic 60.0%
TGATAGGGCTGCTGTGGTGG+TGG - chr4.4:12313874-12313893 None:intergenic 60.0%
TGGCTGCCTCCTGATGAAGG+AGG - chr4.4:12313913-12313932 None:intergenic 60.0%
! TTTGGTGTGAAGGCCGGACG+TGG + chr4.4:12313978-12313997 MS.gene27932:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 12313867 12314313 12313867 ID=MS.gene27932
chr4.4 mRNA 12313867 12314313 12313867 ID=MS.gene27932.t1;Parent=MS.gene27932
chr4.4 exon 12313867 12314313 12313867 ID=MS.gene27932.t1.exon1;Parent=MS.gene27932.t1
chr4.4 CDS 12313867 12314313 12313867 ID=cds.MS.gene27932.t1;Parent=MS.gene27932.t1
Gene Sequence

>MS.gene27932

ATGGCCACCACCACAGCAGCCCTATCAGGAACCATGGTGAGCACCTCCTTCATCAGGAGGCAGCCAATGAACACAACCCTAACAGCATTCCCTAACGTGGGTCAGACTTTATTTGGTGTGAAGGCCGGACGTGGAAGTCGTATCACTGCCATGGCCAGTTACAAGATCAAGCTCATTACCCCAGATGGAACTAAAGAACTTACCTGCCCGGATAATGAGTACATTCTCGACGTAGCCGAGGAACAAGGCATCGATCTTCCTTACTCATGCAGGGCTGGTGCTTGCTCTTCATGTGCTGGAAAAGTTGTAGAAGGGGAAGTGGATAATTCAGATAATAGCTTCCTTGATGATGATCAATTAAGTGCTGGGTTTGTTCTCACATGTGTTGCTTACCCTCGTTCCGACCTTGTTATTGAGACACACAAGGAGGAAGATCTTGTTTCTTAA

Protein sequence

>MS.gene27932.t1

MATTTAALSGTMVSTSFIRRQPMNTTLTAFPNVGQTLFGVKAGRGSRITAMASYKIKLITPDGTKELTCPDNEYILDVAEEQGIDLPYSCRAGACSSCAGKVVEGEVDNSDNSFLDDDQLSAGFVLTCVAYPRSDLVIETHKEEDLVS