Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004833.t1 | XP_003590835.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 5.60E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004833.t1 | P55844 | 91.0 | 133 | 12 | 0 | 1 | 133 | 1 | 133 | 2.2e-61 | 236.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004833.t1 | G7I4V0 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 4.1e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68134 | MS.gene004833 | PPI |
| MS.gene063084 | MS.gene004833 | PPI |
| MS.gene52970 | MS.gene004833 | PPI |
| MS.gene004833 | MS.gene066035 | PPI |
| MS.gene066034 | MS.gene004833 | PPI |
| MS.gene014197 | MS.gene004833 | PPI |
| MS.gene004833 | MS.gene68733 | PPI |
| MS.gene52972 | MS.gene004833 | PPI |
| MS.gene066761 | MS.gene004833 | PPI |
| MS.gene71415 | MS.gene004833 | PPI |
| MS.gene004833 | MS.gene77100 | PPI |
| MS.gene004833 | MS.gene041630 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004833.t1 | MTR_1g075720 | 99.248 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 2.34e-90 | 258 |
| MS.gene004833.t1 | MTR_4g120770 | 95.489 | 133 | 6 | 0 | 1 | 133 | 1 | 133 | 4.44e-89 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene004833.t1 | AT2G20450 | 84.962 | 133 | 20 | 0 | 1 | 133 | 1 | 133 | 7.52e-77 | 224 |
| MS.gene004833.t1 | AT4G27090 | 85.385 | 130 | 19 | 0 | 1 | 130 | 1 | 130 | 6.52e-76 | 221 |
Find 43 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAACCTATCAAAATCATTA+AGG | 0.237759 | 1.4:-61951821 | None:intergenic |
| TTGACGAGATCCTTCTTCTT+AGG | 0.250131 | 1.4:-61951578 | None:intergenic |
| GTCAACTATGGCCCAGATTA+TGG | 0.258615 | 1.4:+61951330 | MS.gene004833:CDS |
| GATTATGTTGGCCAAGATTA+AGG | 0.263288 | 1.4:+61951845 | MS.gene004833:CDS |
| ACAACGAGCTTGCCATAATC+TGG | 0.305463 | 1.4:-61951342 | None:intergenic |
| GAAAATGGGTTACCCTAGTT+TGG | 0.329740 | 1.4:-61951383 | None:intergenic |
| GAAAGCCTCTTGAAATTGAT+TGG | 0.344891 | 1.4:-61951521 | None:intergenic |
| TATCAATCTTGATATCAGTT+AGG | 0.362900 | 1.4:-61951544 | None:intergenic |
| CTAAAATTATACCTTAATCT+TGG | 0.381940 | 1.4:-61951856 | None:intergenic |
| AAAGCCTCTTGAAATTGATT+GGG | 0.387025 | 1.4:-61951520 | None:intergenic |
| TGCAGAGGGCTGGTGTTGTT+AGG | 0.431787 | 1.4:+61952573 | MS.gene004833:intron |
| TTGATGTTATCGACCAAACT+AGG | 0.433748 | 1.4:+61951370 | MS.gene004833:CDS |
| GAACCTATCAAAATCATTAA+GGG | 0.440003 | 1.4:-61951820 | None:intergenic |
| AACAATCAATTTCCTTCCCC+AGG | 0.448020 | 1.4:-61951781 | None:intergenic |
| ATTGGGGCTCTCTCCATGTC+AGG | 0.459706 | 1.4:-61951503 | None:intergenic |
| ATCAATCTTGATATCAGTTA+GGG | 0.462600 | 1.4:-61951543 | None:intergenic |
| AGAGTTGCACTTGTCAACTA+TGG | 0.476767 | 1.4:+61951318 | MS.gene004833:CDS |
| GCTGAGGAAGACCGCAGCTT+AGG | 0.479614 | 1.4:+61952610 | MS.gene004833:CDS |
| TTTCAATTTGTGCAGAGGGC+TGG | 0.483986 | 1.4:+61952563 | MS.gene004833:intron |
| GAAGGATCTCGTCAAGGCTA+TGG | 0.488672 | 1.4:+61951586 | MS.gene004833:CDS |
| GGAAATTGATTGTTAAGAAG+AGG | 0.493248 | 1.4:+61951790 | MS.gene004833:CDS |
| TTCAAGAGGTTTGTTGAGAT+CGG | 0.494020 | 1.4:+61951294 | MS.gene004833:CDS |
| CAAGAGAGTTCCTAAGAAGA+AGG | 0.504705 | 1.4:+61951568 | MS.gene004833:CDS |
| ACAAGTGGGAGAAGAGCTCC+TGG | 0.539704 | 1.4:+61951763 | MS.gene004833:CDS |
| AATTGATTGTTAAGAAGAGG+AGG | 0.539799 | 1.4:+61951793 | MS.gene004833:CDS |
| TGATAGGTTCAAGATTATGT+TGG | 0.542816 | 1.4:+61951833 | MS.gene004833:CDS |
| CAACGAGCTTGCCATAATCT+GGG | 0.548769 | 1.4:-61951341 | None:intergenic |
| TGATAGATGTGAAGAACAAG+TGG | 0.562094 | 1.4:+61951748 | MS.gene004833:intron |
| ATCTCAACAAACCTCTTGAA+CGG | 0.571035 | 1.4:-61951291 | None:intergenic |
| AAGCCTCTTGAAATTGATTG+GGG | 0.580879 | 1.4:-61951519 | None:intergenic |
| GAGCCCCAATCAATTTCAAG+AGG | 0.583215 | 1.4:+61951516 | MS.gene004833:CDS |
| GGGAGAAGAGCTCCTGGGGA+AGG | 0.586483 | 1.4:+61951769 | MS.gene004833:CDS |
| GTCAAGGCTATGGAAGCTGC+AGG | 0.587422 | 1.4:+61951596 | MS.gene004833:CDS |
| TCTTTGTTCAGCCGTTCAAG+AGG | 0.591089 | 1.4:+61951280 | MS.gene004833:intron |
| TCTTGTTGATGCACCTGACA+TGG | 0.596389 | 1.4:+61951490 | MS.gene004833:CDS |
| ATTGATTGTTAAGAAGAGGA+GGG | 0.602431 | 1.4:+61951794 | MS.gene004833:CDS |
| TGATGTTATCGACCAAACTA+GGG | 0.607546 | 1.4:+61951371 | MS.gene004833:CDS |
| GGCAAGAACTTGCTAAGCTG+AGG | 0.613930 | 1.4:+61952594 | MS.gene004833:CDS |
| AAGTGGGAGAAGAGCTCCTG+GGG | 0.619297 | 1.4:+61951765 | MS.gene004833:CDS |
| GATAGATGTGAAGAACAAGT+GGG | 0.623335 | 1.4:+61951749 | MS.gene004833:intron |
| TAAGAAGAAGGATCTCGTCA+AGG | 0.637349 | 1.4:+61951580 | MS.gene004833:CDS |
| CAAGTGGGAGAAGAGCTCCT+GGG | 0.641380 | 1.4:+61951764 | MS.gene004833:CDS |
| ATGAAGTAATGCCTAAGCTG+CGG | 0.676586 | 1.4:-61952621 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATCTGTTATCATAAAATT+TGG | + | chr1.4:61952331-61952350 | MS.gene004833:intron | 15.0% |
| !! | TTTGGTTAAATAACTTAATT+AGG | + | chr1.4:61951927-61951946 | MS.gene004833:intron | 15.0% |
| !!! | AATTTTATGATAACAGATTA+AGG | - | chr1.4:61952331-61952350 | None:intergenic | 15.0% |
| !!! | ATTTTATGATAACAGATTAA+GGG | - | chr1.4:61952330-61952349 | None:intergenic | 15.0% |
| !! | AAAGATTGGAAATTTGAAAA+TGG | - | chr1.4:61951401-61951420 | None:intergenic | 20.0% |
| !! | AAGATTGGAAATTTGAAAAT+GGG | - | chr1.4:61951400-61951419 | None:intergenic | 20.0% |
| !! | ATTAAGTTATTTAACCAAAC+AGG | - | chr1.4:61951926-61951945 | None:intergenic | 20.0% |
| !! | ATTAGAGAAAGATTAAGAAA+TGG | - | chr1.4:61951248-61951267 | None:intergenic | 20.0% |
| !! | CTAAAATTATACCTTAATCT+TGG | - | chr1.4:61951859-61951878 | None:intergenic | 20.0% |
| !! | TTAAGTTATTTAACCAAACA+GGG | - | chr1.4:61951925-61951944 | None:intergenic | 20.0% |
| !! | TTAAGTTATTTATCCAAACA+GGG | - | chr1.4:61952007-61952026 | None:intergenic | 20.0% |
| !! | TTTGGATAAATAACTTAACT+TGG | + | chr1.4:61952009-61952028 | MS.gene004833:intron | 20.0% |
| !!! | ATTGTTTTTGTATAAGCTTT+AGG | + | chr1.4:61952111-61952130 | MS.gene004833:intron | 20.0% |
| !!! | GAAATTGGATTTTAATTTTG+TGG | + | chr1.4:61951705-61951724 | MS.gene004833:intron | 20.0% |
| !!! | GATTTTAATTTTGTGGTAAT+TGG | + | chr1.4:61951712-61951731 | MS.gene004833:intron | 20.0% |
| !!! | TAGCTTTTGTTTATTGAAAT+TGG | + | chr1.4:61951690-61951709 | MS.gene004833:intron | 20.0% |
| !!! | TTGTTTTTGTATAAGCTTTA+GGG | + | chr1.4:61952112-61952131 | MS.gene004833:intron | 20.0% |
| !!! | TTTTCAGAAATTAAGCAAAT+GGG | - | chr1.4:61951441-61951460 | None:intergenic | 20.0% |
| !!! | TTTTTCAGAAATTAAGCAAA+TGG | - | chr1.4:61951442-61951461 | None:intergenic | 20.0% |
| ! | ATCAATCTTGATATCAGTTA+GGG | - | chr1.4:61951546-61951565 | None:intergenic | 25.0% |
| ! | GAACCTATCAAAATCATTAA+GGG | - | chr1.4:61951823-61951842 | None:intergenic | 25.0% |
| ! | GTTAAGTTATTTATCCAAAC+AGG | - | chr1.4:61952008-61952027 | None:intergenic | 25.0% |
| ! | TAAAATAAGCTAATCCAAAC+AGG | + | chr1.4:61952447-61952466 | MS.gene004833:intron | 25.0% |
| ! | TAAATTTCAATTTGTGCAGA+GGG | + | chr1.4:61952559-61952578 | MS.gene004833:intron | 25.0% |
| ! | TATCAATCTTGATATCAGTT+AGG | - | chr1.4:61951547-61951566 | None:intergenic | 25.0% |
| ! | TGAACCTATCAAAATCATTA+AGG | - | chr1.4:61951824-61951843 | None:intergenic | 25.0% |
| ! | TTAAATTTCAATTTGTGCAG+AGG | + | chr1.4:61952558-61952577 | MS.gene004833:intron | 25.0% |
| !! | TTTTCATAAGTCAATCCAAA+CGG | - | chr1.4:61952139-61952158 | None:intergenic | 25.0% |
| !!! | TTGTGAAATGCTTTTTAGTT+AGG | + | chr1.4:61951899-61951918 | MS.gene004833:intron | 25.0% |
| !!! | TTGTGATATGCTTTTAAGTT+AGG | + | chr1.4:61951981-61952000 | MS.gene004833:intron | 25.0% |
| !!! | TTGTTTTTCAAATTGTGTCT+AGG | + | chr1.4:61952498-61952517 | MS.gene004833:intron | 25.0% |
| AAAGCCTCTTGAAATTGATT+GGG | - | chr1.4:61951523-61951542 | None:intergenic | 30.0% | |
| AAAGTTGTATCTTCATAAGC+TGG | + | chr1.4:61952214-61952233 | MS.gene004833:intron | 30.0% | |
| AATTGATTGTTAAGAAGAGG+AGG | + | chr1.4:61951793-61951812 | MS.gene004833:CDS | 30.0% | |
| ATTGATTGTTAAGAAGAGGA+GGG | + | chr1.4:61951794-61951813 | MS.gene004833:CDS | 30.0% | |
| GGAAATTGATTGTTAAGAAG+AGG | + | chr1.4:61951790-61951809 | MS.gene004833:CDS | 30.0% | |
| GGTTGAAAATGGAAAAAGAT+TGG | - | chr1.4:61951415-61951434 | None:intergenic | 30.0% | |
| TAATATTCATGAGCTTGTCA+AGG | - | chr1.4:61952254-61952273 | None:intergenic | 30.0% | |
| TGATAGGTTCAAGATTATGT+TGG | + | chr1.4:61951833-61951852 | MS.gene004833:CDS | 30.0% | |
| ! | AATTAGGCTTTTAGTGTAAG+TGG | + | chr1.4:61951943-61951962 | MS.gene004833:intron | 30.0% |
| !!! | TTGTGATTTGTTTGTTTTGC+AGG | + | chr1.4:61951466-61951485 | MS.gene004833:intron | 30.0% |
| AAACCCTACATACTCAACTA+AGG | - | chr1.4:61952474-61952493 | None:intergenic | 35.0% | |
| AACCCTACATACTCAACTAA+GGG | - | chr1.4:61952473-61952492 | None:intergenic | 35.0% | |
| AAGCCTCTTGAAATTGATTG+GGG | - | chr1.4:61951522-61951541 | None:intergenic | 35.0% | |
| ATCTCAACAAACCTCTTGAA+CGG | - | chr1.4:61951294-61951313 | None:intergenic | 35.0% | |
| GAAAGCCTCTTGAAATTGAT+TGG | - | chr1.4:61951524-61951543 | None:intergenic | 35.0% | |
| GATAGATGTGAAGAACAAGT+GGG | + | chr1.4:61951749-61951768 | MS.gene004833:intron | 35.0% | |
| GATTATGTTGGCCAAGATTA+AGG | + | chr1.4:61951845-61951864 | MS.gene004833:CDS | 35.0% | |
| GGTTATTATACGAGCTGTTA+TGG | + | chr1.4:61952050-61952069 | MS.gene004833:intron | 35.0% | |
| TAACAGATTAAGGGTCTGTT+CGG | - | chr1.4:61952321-61952340 | None:intergenic | 35.0% | |
| TGATAGATGTGAAGAACAAG+TGG | + | chr1.4:61951748-61951767 | MS.gene004833:intron | 35.0% | |
| TGATGTTATCGACCAAACTA+GGG | + | chr1.4:61951371-61951390 | MS.gene004833:CDS | 35.0% | |
| TGGAGATTATTGTGTAAGTG+TGG | + | chr1.4:61952029-61952048 | MS.gene004833:intron | 35.0% | |
| TTGATGTTATCGACCAAACT+AGG | + | chr1.4:61951370-61951389 | MS.gene004833:CDS | 35.0% | |
| ! | AGAAATTAAGCAAATGGGCT+AGG | - | chr1.4:61951436-61951455 | None:intergenic | 35.0% |
| !! | CTGCCCTTAATGATTTTGAT+AGG | + | chr1.4:61951817-61951836 | MS.gene004833:CDS | 35.0% |
| !! | TTCAAGAGGTTTGTTGAGAT+CGG | + | chr1.4:61951294-61951313 | MS.gene004833:CDS | 35.0% |
| AACAATCAATTTCCTTCCCC+AGG | - | chr1.4:61951784-61951803 | None:intergenic | 40.0% | |
| AGAGTTGCACTTGTCAACTA+TGG | + | chr1.4:61951318-61951337 | MS.gene004833:CDS | 40.0% | |
| CAAGAGAGTTCCTAAGAAGA+AGG | + | chr1.4:61951568-61951587 | MS.gene004833:CDS | 40.0% | |
| TAAGAAGAAGGATCTCGTCA+AGG | + | chr1.4:61951580-61951599 | MS.gene004833:CDS | 40.0% | |
| TGTCTGTGAGTACTTATGTG+TGG | - | chr1.4:61952398-61952417 | None:intergenic | 40.0% | |
| TTGACGAGATCCTTCTTCTT+AGG | - | chr1.4:61951581-61951600 | None:intergenic | 40.0% | |
| ! | CAGACAGTCTTACATGCTTT+TGG | + | chr1.4:61952412-61952431 | MS.gene004833:intron | 40.0% |
| !! | CAAATGGGCTAGGTTGAAAA+TGG | - | chr1.4:61951426-61951445 | None:intergenic | 40.0% |
| !! | GAAAATGGGTTACCCTAGTT+TGG | - | chr1.4:61951386-61951405 | None:intergenic | 40.0% |
| !! | TATAAGCTTTAGGGTCCGTT+TGG | + | chr1.4:61952121-61952140 | MS.gene004833:intron | 40.0% |
| !!! | CTTTTAAGTTAGGCCCTGTT+TGG | + | chr1.4:61951991-61952010 | MS.gene004833:intron | 40.0% |
| !!! | CTTTTTAGTTAGGCCCTGTT+TGG | + | chr1.4:61951909-61951928 | MS.gene004833:intron | 40.0% |
| ACAACGAGCTTGCCATAATC+TGG | - | chr1.4:61951345-61951364 | None:intergenic | 45.0% | |
| AGGCCCTTAGTTGAGTATGT+AGG | + | chr1.4:61952467-61952486 | MS.gene004833:intron | 45.0% | |
| CAACGAGCTTGCCATAATCT+GGG | - | chr1.4:61951344-61951363 | None:intergenic | 45.0% | |
| GAGCCCCAATCAATTTCAAG+AGG | + | chr1.4:61951516-61951535 | MS.gene004833:CDS | 45.0% | |
| GATTAAGGGTCTGTTCGGAT+TGG | - | chr1.4:61952316-61952335 | None:intergenic | 45.0% | |
| GGCCCTTAGTTGAGTATGTA+GGG | + | chr1.4:61952468-61952487 | MS.gene004833:intron | 45.0% | |
| GTCAACTATGGCCCAGATTA+TGG | + | chr1.4:61951330-61951349 | MS.gene004833:CDS | 45.0% | |
| TACTCAACTAAGGGCCTGTT+TGG | - | chr1.4:61952464-61952483 | None:intergenic | 45.0% | |
| TCTTGTTGATGCACCTGACA+TGG | + | chr1.4:61951490-61951509 | MS.gene004833:CDS | 45.0% | |
| TCTTTGTTCAGCCGTTCAAG+AGG | + | chr1.4:61951280-61951299 | MS.gene004833:intron | 45.0% | |
| TTTCAATTTGTGCAGAGGGC+TGG | + | chr1.4:61952563-61952582 | MS.gene004833:intron | 45.0% | |
| !! | AAATAAATAAATATATAGAT+AGG | - | chr1.4:61951637-61951656 | None:intergenic | 5.0% |
| GAAGGATCTCGTCAAGGCTA+TGG | + | chr1.4:61951586-61951605 | MS.gene004833:CDS | 50.0% | |
| !! | GGCAAGAACTTGCTAAGCTG+AGG | + | chr1.4:61952594-61952613 | MS.gene004833:CDS | 50.0% |
| AAGTGGGAGAAGAGCTCCTG+GGG | + | chr1.4:61951765-61951784 | MS.gene004833:CDS | 55.0% | |
| ACAAGTGGGAGAAGAGCTCC+TGG | + | chr1.4:61951763-61951782 | MS.gene004833:CDS | 55.0% | |
| ATTGGGGCTCTCTCCATGTC+AGG | - | chr1.4:61951506-61951525 | None:intergenic | 55.0% | |
| CAAGTGGGAGAAGAGCTCCT+GGG | + | chr1.4:61951764-61951783 | MS.gene004833:CDS | 55.0% | |
| GTCAAGGCTATGGAAGCTGC+AGG | + | chr1.4:61951596-61951615 | MS.gene004833:CDS | 55.0% | |
| !! | TGCAGAGGGCTGGTGTTGTT+AGG | + | chr1.4:61952573-61952592 | MS.gene004833:intron | 55.0% |
| GGGAGAAGAGCTCCTGGGGA+AGG | + | chr1.4:61951769-61951788 | MS.gene004833:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 61951206 | 61952631 | 61951206 | ID=MS.gene004833 |
| chr1.4 | mRNA | 61951206 | 61952631 | 61951206 | ID=MS.gene004833.t1;Parent=MS.gene004833 |
| chr1.4 | exon | 61951206 | 61951208 | 61951206 | ID=MS.gene004833.t1.exon1;Parent=MS.gene004833.t1 |
| chr1.4 | CDS | 61951206 | 61951208 | 61951206 | ID=cds.MS.gene004833.t1;Parent=MS.gene004833.t1 |
| chr1.4 | exon | 61951291 | 61951392 | 61951291 | ID=MS.gene004833.t1.exon2;Parent=MS.gene004833.t1 |
| chr1.4 | CDS | 61951291 | 61951392 | 61951291 | ID=cds.MS.gene004833.t1;Parent=MS.gene004833.t1 |
| chr1.4 | exon | 61951488 | 61951617 | 61951488 | ID=MS.gene004833.t1.exon3;Parent=MS.gene004833.t1 |
| chr1.4 | CDS | 61951488 | 61951617 | 61951488 | ID=cds.MS.gene004833.t1;Parent=MS.gene004833.t1 |
| chr1.4 | exon | 61951754 | 61951866 | 61951754 | ID=MS.gene004833.t1.exon4;Parent=MS.gene004833.t1 |
| chr1.4 | CDS | 61951754 | 61951866 | 61951754 | ID=cds.MS.gene004833.t1;Parent=MS.gene004833.t1 |
| chr1.4 | exon | 61952578 | 61952631 | 61952578 | ID=MS.gene004833.t1.exon5;Parent=MS.gene004833.t1 |
| chr1.4 | CDS | 61952578 | 61952631 | 61952578 | ID=cds.MS.gene004833.t1;Parent=MS.gene004833.t1 |
| Gene Sequence |
| Protein sequence |
