Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52972.t1 | XP_003590835.1 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 5.60E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52972.t1 | P55844 | 91.0 | 133 | 12 | 0 | 1 | 133 | 1 | 133 | 2.2e-61 | 236.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52972.t1 | G7I4V0 | 99.2 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 4.1e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68134 | MS.gene52972 | PPI |
| MS.gene52972 | MS.gene71415 | PPI |
| MS.gene52970 | MS.gene52972 | PPI |
| MS.gene066034 | MS.gene52972 | PPI |
| MS.gene014197 | MS.gene52972 | PPI |
| MS.gene52972 | MS.gene066035 | PPI |
| MS.gene52972 | MS.gene066761 | PPI |
| MS.gene52972 | MS.gene004833 | PPI |
| MS.gene52972 | MS.gene68733 | PPI |
| MS.gene52972 | MS.gene77100 | PPI |
| MS.gene52972 | MS.gene041630 | PPI |
| MS.gene52972 | MS.gene063084 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52972.t1 | MTR_1g075720 | 99.248 | 133 | 1 | 0 | 1 | 133 | 1 | 133 | 2.34e-90 | 258 |
| MS.gene52972.t1 | MTR_4g120770 | 95.489 | 133 | 6 | 0 | 1 | 133 | 1 | 133 | 4.44e-89 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52972.t1 | AT2G20450 | 84.962 | 133 | 20 | 0 | 1 | 133 | 1 | 133 | 7.52e-77 | 224 |
| MS.gene52972.t1 | AT4G27090 | 85.385 | 130 | 19 | 0 | 1 | 130 | 1 | 130 | 6.52e-76 | 221 |
Find 39 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAACCTATCAAAATCATTA+AGG | 0.237759 | 1.1:-56651566 | None:intergenic |
| TTGACGAGATCCTTCTTCTT+AGG | 0.250131 | 1.1:-56651330 | None:intergenic |
| GTCAACTATGGCCCAGATTA+TGG | 0.258615 | 1.1:+56651083 | MS.gene52972:CDS |
| GATTATGTTGGCCAAGATTA+AGG | 0.263288 | 1.1:+56651590 | MS.gene52972:CDS |
| ACAACGAGCTTGCCATAATC+TGG | 0.305463 | 1.1:-56651095 | None:intergenic |
| GAAAATGGGTTACCCTAGTT+TGG | 0.329740 | 1.1:-56651136 | None:intergenic |
| GAAAGCCTCTTGAAATTGAT+TGG | 0.348542 | 1.1:-56651273 | None:intergenic |
| CTAAAATTATACCTTAATCT+TGG | 0.381940 | 1.1:-56651601 | None:intergenic |
| TGCAGAGGGCTGGTGTTGTT+AGG | 0.431787 | 1.1:+56652317 | MS.gene52972:intron |
| TTGATGTTATCGACCAAACT+AGG | 0.433748 | 1.1:+56651123 | MS.gene52972:CDS |
| GAACCTATCAAAATCATTAA+GGG | 0.440003 | 1.1:-56651565 | None:intergenic |
| AACAATCAATTTCCTTCCCC+AGG | 0.448020 | 1.1:-56651526 | None:intergenic |
| ATTGGAGCTCTCTCCATGTC+AGG | 0.463357 | 1.1:-56651255 | None:intergenic |
| GCTGAGGAAGACCGCTGCTT+AGG | 0.472651 | 1.1:+56652354 | MS.gene52972:CDS |
| AGAGTTGCACTTGTCAACTA+TGG | 0.476767 | 1.1:+56651071 | MS.gene52972:CDS |
| TTTCAATTTGTGCAGAGGGC+TGG | 0.483986 | 1.1:+56652307 | MS.gene52972:intron |
| GAAGGATCTCGTCAAGGCTA+TGG | 0.488672 | 1.1:+56651338 | MS.gene52972:CDS |
| GGAAATTGATTGTTAAGAAG+AGG | 0.493248 | 1.1:+56651535 | MS.gene52972:CDS |
| TTCAAGAGGTTTGTTGAGAT+CGG | 0.494020 | 1.1:+56651047 | MS.gene52972:CDS |
| CAAGAGAGTTCCTAAGAAGA+AGG | 0.504705 | 1.1:+56651320 | MS.gene52972:CDS |
| ACAAGTGGGAGAAGAGCTCC+TGG | 0.539704 | 1.1:+56651508 | MS.gene52972:CDS |
| AATTGATTGTTAAGAAGAGG+AGG | 0.539799 | 1.1:+56651538 | MS.gene52972:CDS |
| TGATAGGTTCAAGATTATGT+TGG | 0.542816 | 1.1:+56651578 | MS.gene52972:CDS |
| CAACGAGCTTGCCATAATCT+GGG | 0.548769 | 1.1:-56651094 | None:intergenic |
| TGATAGATGTGAAGAACAAG+TGG | 0.562094 | 1.1:+56651493 | MS.gene52972:intron |
| ATCTCAACAAACCTCTTGAA+CGG | 0.571035 | 1.1:-56651044 | None:intergenic |
| GAGCTCCAATCAATTTCAAG+AGG | 0.584468 | 1.1:+56651268 | MS.gene52972:CDS |
| GGGAGAAGAGCTCCTGGGGA+AGG | 0.586483 | 1.1:+56651514 | MS.gene52972:CDS |
| GTCAAGGCTATGGAAGCTGC+AGG | 0.587422 | 1.1:+56651348 | MS.gene52972:CDS |
| TCTTTGTTCAGCCGTTCAAG+AGG | 0.591089 | 1.1:+56651033 | MS.gene52972:intron |
| TCTTGTTGATGCACCTGACA+TGG | 0.596389 | 1.1:+56651242 | MS.gene52972:CDS |
| ATTGATTGTTAAGAAGAGGA+GGG | 0.602431 | 1.1:+56651539 | MS.gene52972:CDS |
| TGATGTTATCGACCAAACTA+GGG | 0.607546 | 1.1:+56651124 | MS.gene52972:CDS |
| GGCAAGAACTTGCTAAGCTG+AGG | 0.613930 | 1.1:+56652338 | MS.gene52972:CDS |
| AAGTGGGAGAAGAGCTCCTG+GGG | 0.619297 | 1.1:+56651510 | MS.gene52972:CDS |
| GATAGATGTGAAGAACAAGT+GGG | 0.623335 | 1.1:+56651494 | MS.gene52972:intron |
| TAAGAAGAAGGATCTCGTCA+AGG | 0.637349 | 1.1:+56651332 | MS.gene52972:CDS |
| CAAGTGGGAGAAGAGCTCCT+GGG | 0.641380 | 1.1:+56651509 | MS.gene52972:CDS |
| ATGAAGTAATGCCTAAGCAG+CGG | 0.686386 | 1.1:-56652365 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAATAAATATATAGAT+AGG | - | chr1.1:56651389-56651408 | None:intergenic | 10.0% |
| !! | TATGTTCAAAAATTGTTAAA+TGG | - | chr1.1:56652140-56652159 | None:intergenic | 15.0% |
| !! | TTTGGTTAAATAACTTAATT+AGG | + | chr1.1:56651672-56651691 | MS.gene52972:intron | 15.0% |
| !! | AAAGATTGGAAATTTGAAAA+TGG | - | chr1.1:56651154-56651173 | None:intergenic | 20.0% |
| !! | AAGATTGGAAATTTGAAAAT+GGG | - | chr1.1:56651153-56651172 | None:intergenic | 20.0% |
| !! | ATTAGAGAAAGATTAAGAAA+TGG | - | chr1.1:56651001-56651020 | None:intergenic | 20.0% |
| !! | CTAAAATTATACCTTAATCT+TGG | - | chr1.1:56651604-56651623 | None:intergenic | 20.0% |
| !! | TTAAGTTATTTAACCAAACA+AGG | - | chr1.1:56651670-56651689 | None:intergenic | 20.0% |
| !! | TTAAGTTATTTATCCAAACA+GGG | - | chr1.1:56651752-56651771 | None:intergenic | 20.0% |
| !! | TTTGGATAAATAACTTAACT+TGG | + | chr1.1:56651754-56651773 | MS.gene52972:intron | 20.0% |
| !!! | AAAATGAAGTCAAATTGTTT+TGG | + | chr1.1:56651843-56651862 | MS.gene52972:intron | 20.0% |
| !!! | AATCTGTTTCTTAAGTTATT+TGG | + | chr1.1:56652075-56652094 | MS.gene52972:intron | 20.0% |
| !!! | TAGCTTTTTTTTGTTGAAAT+TGG | + | chr1.1:56651437-56651456 | MS.gene52972:intron | 20.0% |
| !!! | TGTTTTTTCAAATTGTGTTT+TGG | + | chr1.1:56652246-56652265 | MS.gene52972:intron | 20.0% |
| !!! | TTGAAATTGGATTTTAATTG+TGG | + | chr1.1:56651450-56651469 | MS.gene52972:intron | 20.0% |
| !!! | TTGTGAAATGATTTTTAGTT+AGG | + | chr1.1:56651644-56651663 | MS.gene52972:intron | 20.0% |
| !!! | TTTTCAGAAATTAAGCAAAT+GGG | - | chr1.1:56651194-56651213 | None:intergenic | 20.0% |
| !!! | TTTTTCAGAAATTAAGCAAA+TGG | - | chr1.1:56651195-56651214 | None:intergenic | 20.0% |
| ! | ATAATTTCAATTTGTGCAGA+GGG | + | chr1.1:56652303-56652322 | MS.gene52972:intron | 25.0% |
| ! | GAACCTATCAAAATCATTAA+GGG | - | chr1.1:56651568-56651587 | None:intergenic | 25.0% |
| ! | GTTAAGTTATTTATCCAAAC+AGG | - | chr1.1:56651753-56651772 | None:intergenic | 25.0% |
| ! | GTTCATTTGACATTGTTAAA+TGG | - | chr1.1:56652118-56652137 | None:intergenic | 25.0% |
| ! | TGAACCTATCAAAATCATTA+AGG | - | chr1.1:56651569-56651588 | None:intergenic | 25.0% |
| ! | TTTCAGAAATTAAGCAAATG+GGG | - | chr1.1:56651193-56651212 | None:intergenic | 25.0% |
| !! | TTTTCATAAGTCAATCCAAA+CGG | - | chr1.1:56651884-56651903 | None:intergenic | 25.0% |
| !!! | ATTGTTTTGGTATAAGCTTT+AGG | + | chr1.1:56651856-56651875 | MS.gene52972:intron | 25.0% |
| !!! | TGGATTTTAATTGTGGTAAT+TGG | + | chr1.1:56651457-56651476 | MS.gene52972:intron | 25.0% |
| !!! | TTGTGAAATGCTTTTAAGTT+AGG | + | chr1.1:56651726-56651745 | MS.gene52972:intron | 25.0% |
| !!! | TTGTTTTGGTATAAGCTTTA+GGG | + | chr1.1:56651857-56651876 | MS.gene52972:intron | 25.0% |
| AAAGTTGTATCTTCATAAGC+TGG | + | chr1.1:56651959-56651978 | MS.gene52972:intron | 30.0% | |
| AATTGATTGTTAAGAAGAGG+AGG | + | chr1.1:56651538-56651557 | MS.gene52972:CDS | 30.0% | |
| ATTGATTGTTAAGAAGAGGA+GGG | + | chr1.1:56651539-56651558 | MS.gene52972:CDS | 30.0% | |
| CATAATTTCAATTTGTGCAG+AGG | + | chr1.1:56652302-56652321 | MS.gene52972:intron | 30.0% | |
| GGAAATTGATTGTTAAGAAG+AGG | + | chr1.1:56651535-56651554 | MS.gene52972:CDS | 30.0% | |
| GGTTGCAAATAGAAAAAGAT+TGG | - | chr1.1:56651168-56651187 | None:intergenic | 30.0% | |
| TAATATTCATGAGCTTGTCA+AGG | - | chr1.1:56651999-56652018 | None:intergenic | 30.0% | |
| TGATAGGTTCAAGATTATGT+TGG | + | chr1.1:56651578-56651597 | MS.gene52972:CDS | 30.0% | |
| ! | AATTAGGCTTTTAGTGTAAG+TGG | + | chr1.1:56651688-56651707 | MS.gene52972:intron | 30.0% |
| !!! | ATTTTTAGTTAGGCCTTGTT+TGG | + | chr1.1:56651654-56651673 | MS.gene52972:intron | 30.0% |
| !!! | TTGTGATTTGTTTGTTTTGC+AGG | + | chr1.1:56651218-56651237 | MS.gene52972:intron | 30.0% |
| AAACCCTATAAACTCAGCTA+AGG | - | chr1.1:56652221-56652240 | None:intergenic | 35.0% | |
| AACCCTATAAACTCAGCTAA+GGG | - | chr1.1:56652220-56652239 | None:intergenic | 35.0% | |
| ACCAACTTGAAACTTATGTG+TGG | - | chr1.1:56652163-56652182 | None:intergenic | 35.0% | |
| AGAAATTAAGCAAATGGGGT+TGG | - | chr1.1:56651189-56651208 | None:intergenic | 35.0% | |
| ATCTCAACAAACCTCTTGAA+CGG | - | chr1.1:56651047-56651066 | None:intergenic | 35.0% | |
| CAGATTAAGTCTGTTCAGAT+TGG | - | chr1.1:56652061-56652080 | None:intergenic | 35.0% | |
| GAAAGCCTCTTGAAATTGAT+TGG | - | chr1.1:56651276-56651295 | None:intergenic | 35.0% | |
| GATAGATGTGAAGAACAAGT+GGG | + | chr1.1:56651494-56651513 | MS.gene52972:intron | 35.0% | |
| GATTATGTTGGCCAAGATTA+AGG | + | chr1.1:56651590-56651609 | MS.gene52972:CDS | 35.0% | |
| GGTTATTATACGAGCTGTTA+TGG | + | chr1.1:56651795-56651814 | MS.gene52972:intron | 35.0% | |
| TGATAGATGTGAAGAACAAG+TGG | + | chr1.1:56651493-56651512 | MS.gene52972:intron | 35.0% | |
| TGATGTTATCGACCAAACTA+GGG | + | chr1.1:56651124-56651143 | MS.gene52972:CDS | 35.0% | |
| TGGAGCTTATTGTGTAAATG+TGG | + | chr1.1:56651774-56651793 | MS.gene52972:intron | 35.0% | |
| TTGATGTTATCGACCAAACT+AGG | + | chr1.1:56651123-56651142 | MS.gene52972:CDS | 35.0% | |
| ! | ACCACACATAAGTTTCAAGT+TGG | + | chr1.1:56652159-56652178 | MS.gene52972:intron | 35.0% |
| !! | CTGCCCTTAATGATTTTGAT+AGG | + | chr1.1:56651562-56651581 | MS.gene52972:CDS | 35.0% |
| !! | TTCAAGAGGTTTGTTGAGAT+CGG | + | chr1.1:56651047-56651066 | MS.gene52972:CDS | 35.0% |
| AACAATCAATTTCCTTCCCC+AGG | - | chr1.1:56651529-56651548 | None:intergenic | 40.0% | |
| AAGCCCTTAGCTGAGTTTAT+AGG | + | chr1.1:56652214-56652233 | MS.gene52972:intron | 40.0% | |
| AGAGTTGCACTTGTCAACTA+TGG | + | chr1.1:56651071-56651090 | MS.gene52972:CDS | 40.0% | |
| AGCCCTTAGCTGAGTTTATA+GGG | + | chr1.1:56652215-56652234 | MS.gene52972:intron | 40.0% | |
| CAAGAGAGTTCCTAAGAAGA+AGG | + | chr1.1:56651320-56651339 | MS.gene52972:CDS | 40.0% | |
| GAGCTCCAATCAATTTCAAG+AGG | + | chr1.1:56651268-56651287 | MS.gene52972:CDS | 40.0% | |
| TAAGAAGAAGGATCTCGTCA+AGG | + | chr1.1:56651332-56651351 | MS.gene52972:CDS | 40.0% | |
| TTGACGAGATCCTTCTTCTT+AGG | - | chr1.1:56651333-56651352 | None:intergenic | 40.0% | |
| !! | GAAAATGGGTTACCCTAGTT+TGG | - | chr1.1:56651139-56651158 | None:intergenic | 40.0% |
| !! | TATAAGCTTTAGGGTCCGTT+TGG | + | chr1.1:56651866-56651885 | MS.gene52972:intron | 40.0% |
| !!! | CTTTTAAGTTAGGCCCTGTT+TGG | + | chr1.1:56651736-56651755 | MS.gene52972:intron | 40.0% |
| ACAACGAGCTTGCCATAATC+TGG | - | chr1.1:56651098-56651117 | None:intergenic | 45.0% | |
| CAACGAGCTTGCCATAATCT+GGG | - | chr1.1:56651097-56651116 | None:intergenic | 45.0% | |
| GTCAACTATGGCCCAGATTA+TGG | + | chr1.1:56651083-56651102 | MS.gene52972:CDS | 45.0% | |
| TCTTGTTGATGCACCTGACA+TGG | + | chr1.1:56651242-56651261 | MS.gene52972:CDS | 45.0% | |
| TCTTTGTTCAGCCGTTCAAG+AGG | + | chr1.1:56651033-56651052 | MS.gene52972:intron | 45.0% | |
| TTTCAATTTGTGCAGAGGGC+TGG | + | chr1.1:56652307-56652326 | MS.gene52972:intron | 45.0% | |
| ! | AACTCAGCTAAGGGCTTGTT+TGG | - | chr1.1:56652211-56652230 | None:intergenic | 45.0% |
| ! | AGCTAAGGGCTTGTTTGGAT+TGG | - | chr1.1:56652206-56652225 | None:intergenic | 45.0% |
| ATTGGAGCTCTCTCCATGTC+AGG | - | chr1.1:56651258-56651277 | None:intergenic | 50.0% | |
| GAAGGATCTCGTCAAGGCTA+TGG | + | chr1.1:56651338-56651357 | MS.gene52972:CDS | 50.0% | |
| !! | GGCAAGAACTTGCTAAGCTG+AGG | + | chr1.1:56652338-56652357 | MS.gene52972:CDS | 50.0% |
| AAGTGGGAGAAGAGCTCCTG+GGG | + | chr1.1:56651510-56651529 | MS.gene52972:CDS | 55.0% | |
| ACAAGTGGGAGAAGAGCTCC+TGG | + | chr1.1:56651508-56651527 | MS.gene52972:CDS | 55.0% | |
| CAAGTGGGAGAAGAGCTCCT+GGG | + | chr1.1:56651509-56651528 | MS.gene52972:CDS | 55.0% | |
| GTCAAGGCTATGGAAGCTGC+AGG | + | chr1.1:56651348-56651367 | MS.gene52972:CDS | 55.0% | |
| !! | TGCAGAGGGCTGGTGTTGTT+AGG | + | chr1.1:56652317-56652336 | MS.gene52972:intron | 55.0% |
| GGGAGAAGAGCTCCTGGGGA+AGG | + | chr1.1:56651514-56651533 | MS.gene52972:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 56650959 | 56652375 | 56650959 | ID=MS.gene52972 |
| chr1.1 | mRNA | 56650959 | 56652375 | 56650959 | ID=MS.gene52972.t1;Parent=MS.gene52972 |
| chr1.1 | exon | 56650959 | 56650961 | 56650959 | ID=MS.gene52972.t1.exon1;Parent=MS.gene52972.t1 |
| chr1.1 | CDS | 56650959 | 56650961 | 56650959 | ID=cds.MS.gene52972.t1;Parent=MS.gene52972.t1 |
| chr1.1 | exon | 56651044 | 56651145 | 56651044 | ID=MS.gene52972.t1.exon2;Parent=MS.gene52972.t1 |
| chr1.1 | CDS | 56651044 | 56651145 | 56651044 | ID=cds.MS.gene52972.t1;Parent=MS.gene52972.t1 |
| chr1.1 | exon | 56651240 | 56651369 | 56651240 | ID=MS.gene52972.t1.exon3;Parent=MS.gene52972.t1 |
| chr1.1 | CDS | 56651240 | 56651369 | 56651240 | ID=cds.MS.gene52972.t1;Parent=MS.gene52972.t1 |
| chr1.1 | exon | 56651499 | 56651611 | 56651499 | ID=MS.gene52972.t1.exon4;Parent=MS.gene52972.t1 |
| chr1.1 | CDS | 56651499 | 56651611 | 56651499 | ID=cds.MS.gene52972.t1;Parent=MS.gene52972.t1 |
| chr1.1 | exon | 56652322 | 56652375 | 56652322 | ID=MS.gene52972.t1.exon5;Parent=MS.gene52972.t1 |
| chr1.1 | CDS | 56652322 | 56652375 | 56652322 | ID=cds.MS.gene52972.t1;Parent=MS.gene52972.t1 |
| Gene Sequence |
| Protein sequence |
