Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68134.t1 | XP_020229252.1 | 91.7 | 193 | 14 | 2 | 1 | 193 | 1 | 191 | 2.10E-91 | 345.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68134.t1 | Q9LS09 | 86.4 | 169 | 22 | 1 | 1 | 168 | 1 | 169 | 3.1e-80 | 299.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68134.t1 | A0A1S3U1V4 | 95.6 | 180 | 7 | 1 | 1 | 180 | 1 | 179 | 4.3e-91 | 343.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene68134 | MS.gene069975 | PPI |
| MS.gene68134 | MS.gene52972 | PPI |
| MS.gene68134 | MS.gene71415 | PPI |
| MS.gene68134 | MS.gene066034 | PPI |
| MS.gene68134 | MS.gene94307 | PPI |
| MS.gene68134 | MS.gene004833 | PPI |
| MS.gene68134 | MS.gene98552 | PPI |
| MS.gene68134 | MS.gene041630 | PPI |
| MS.gene68134 | MS.gene064876 | PPI |
| MS.gene68134 | MS.gene81646 | PPI |
| MS.gene52970 | MS.gene68134 | PPI |
| MS.gene014301 | MS.gene68134 | PPI |
| MS.gene68134 | MS.gene66836 | PPI |
| MS.gene68134 | MS.gene070538 | PPI |
| MS.gene016712 | MS.gene68134 | PPI |
| MS.gene68134 | MS.gene94315 | PPI |
| MS.gene68134 | MS.gene066035 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68134.t1 | MTR_3g118150 | 96.373 | 193 | 7 | 0 | 1 | 193 | 1 | 193 | 5.38e-135 | 375 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68134.t1 | AT5G38110 | 86.391 | 169 | 22 | 1 | 1 | 168 | 1 | 169 | 1.13e-104 | 300 |
| MS.gene68134.t1 | AT1G66740 | 80.899 | 178 | 31 | 1 | 1 | 178 | 1 | 175 | 2.33e-103 | 296 |
Find 49 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTACTCACATGCTCTTATT+TGG | 0.139177 | 3.1:+92859337 | MS.gene68134:CDS |
| TGGATCTTGTTCTCCAGTTT+GGG | 0.231870 | 3.1:-92859564 | None:intergenic |
| GTGGATCTTGTTCTCCAGTT+TGG | 0.266545 | 3.1:-92859565 | None:intergenic |
| CTACTCACATGCTCTTATTT+GGG | 0.286175 | 3.1:+92859338 | MS.gene68134:CDS |
| GATATCTTCTTCACGAATCT+TGG | 0.287365 | 3.1:-92859297 | None:intergenic |
| TGAGACTTATGACCAGTTAT+TGG | 0.289525 | 3.1:+92859202 | MS.gene68134:CDS |
| CGAGATTTCCTATGAGTGTT+TGG | 0.353611 | 3.1:+92858844 | MS.gene68134:CDS |
| GGGTAGTTGCTCTGAGGGAG+GGG | 0.371206 | 3.1:-92859543 | None:intergenic |
| TGGGGTAGTTGCTCTGAGGG+AGG | 0.378053 | 3.1:-92859545 | None:intergenic |
| GGGGTAGTTGCTCTGAGGGA+GGG | 0.392562 | 3.1:-92859544 | None:intergenic |
| GTTCTCAGGGTGGAAATTGA+TGG | 0.403676 | 3.1:-92859501 | None:intergenic |
| GGTAGTTGCTCTGAGGGAGG+GGG | 0.407080 | 3.1:-92859542 | None:intergenic |
| CAAGGACACTCTCCAATAAC+TGG | 0.417188 | 3.1:-92859214 | None:intergenic |
| TCACGAATCTTGGATGGGTC+TGG | 0.425679 | 3.1:-92859287 | None:intergenic |
| TCAGAGCAACTACCCCAAAC+TGG | 0.436456 | 3.1:+92859551 | MS.gene68134:CDS |
| GAGAGCGGCCAAACACTCAT+AGG | 0.445137 | 3.1:-92858852 | None:intergenic |
| CTATCTGTTAAAACCTTGGT+TGG | 0.449270 | 3.1:-92859431 | None:intergenic |
| AACCCTATCTGTTAAAACCT+TGG | 0.452925 | 3.1:-92859435 | None:intergenic |
| TTATTGGAGAGTGTCCTTGT+TGG | 0.475012 | 3.1:+92859218 | MS.gene68134:CDS |
| TTCTCAGGGTGGAAATTGAT+GGG | 0.479943 | 3.1:-92859500 | None:intergenic |
| TCTGTTAAAACCTTGGTTGG+AGG | 0.483580 | 3.1:-92859428 | None:intergenic |
| GAATGGAAGCTGATTTATGT+TGG | 0.485287 | 3.1:+92859167 | MS.gene68134:CDS |
| TCTTCTTCACGAATCTTGGA+TGG | 0.488809 | 3.1:-92859293 | None:intergenic |
| ATGGGGAACTTAGTGACCCT+TGG | 0.490528 | 3.1:-92859482 | None:intergenic |
| GGTCAAGAGTTTATTCGTGT+TGG | 0.494710 | 3.1:+92859359 | MS.gene68134:CDS |
| CTTGTTGGTCCTGTCAATGT+TGG | 0.502550 | 3.1:+92859233 | MS.gene68134:CDS |
| CGATAGTTTCCAACATTGAC+AGG | 0.504203 | 3.1:-92859242 | None:intergenic |
| TAGGAAATCTCGAACTGAAA+GGG | 0.513564 | 3.1:-92858833 | None:intergenic |
| AAGGGATTGAGAAACGAAGC+TGG | 0.517879 | 3.1:-92858815 | None:intergenic |
| GTTTGGGGTAGTTGCTCTGA+GGG | 0.518854 | 3.1:-92859548 | None:intergenic |
| GAGAGAGGAACCTCCAACCA+AGG | 0.524167 | 3.1:+92859418 | MS.gene68134:CDS |
| ATTCGTGAAGAAGATATCAT+TGG | 0.528304 | 3.1:+92859302 | MS.gene68134:CDS |
| CAATTGTCACAGATTTGGAA+TGG | 0.533603 | 3.1:+92859150 | MS.gene68134:intron |
| ATAGGAAATCTCGAACTGAA+AGG | 0.536457 | 3.1:-92858834 | None:intergenic |
| CGAATCTTGGATGGGTCTGG+TGG | 0.541608 | 3.1:-92859284 | None:intergenic |
| TGTTTGGCCGCTCTCAAAGA+TGG | 0.557561 | 3.1:+92858860 | MS.gene68134:CDS |
| CTTCTTCACGAATCTTGGAT+GGG | 0.564124 | 3.1:-92859292 | None:intergenic |
| GGATCTTGTTCTCCAGTTTG+GGG | 0.567661 | 3.1:-92859563 | None:intergenic |
| AGTTTGGGGTAGTTGCTCTG+AGG | 0.582073 | 3.1:-92859549 | None:intergenic |
| GTATCGGGTGCAGCAGCAAG+TGG | 0.584533 | 3.1:-92859584 | None:intergenic |
| CAACATTGACAGGACCAACA+AGG | 0.622504 | 3.1:-92859232 | None:intergenic |
| TGACGACGAGCAGCTGAGAG+AGG | 0.624627 | 3.1:+92859403 | MS.gene68134:CDS |
| ACATCTTATCTGATAAACCA+AGG | 0.636524 | 3.1:+92859465 | MS.gene68134:CDS |
| GATTTATGTTGGATCTGCTG+AGG | 0.636648 | 3.1:+92859178 | MS.gene68134:CDS |
| GAAACGAAGCTGGATTGTCA+AGG | 0.636849 | 3.1:-92858805 | None:intergenic |
| CATCTTATCTGATAAACCAA+GGG | 0.651929 | 3.1:+92859466 | MS.gene68134:CDS |
| TCTCAGGGTGGAAATTGATG+GGG | 0.674973 | 3.1:-92859499 | None:intergenic |
| GGTTAACATCACGAACGTGA+CGG | 0.680811 | 3.1:+92858781 | MS.gene68134:CDS |
| ATACATACCATCTTTGAGAG+CGG | 0.695677 | 3.1:-92858867 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTACTCTACTTTTATTTTA+GGG | + | chr3.1:92858898-92858917 | MS.gene68134:intron | 15.0% |
| !! | AAACAAAATTAGGTCAAATT+TGG | - | chr3.1:92859005-92859024 | None:intergenic | 20.0% |
| !! | AACAAAATTAGGTCAAATTT+GGG | - | chr3.1:92859004-92859023 | None:intergenic | 20.0% |
| !! | ACTAAGTAACAAACAAAATT+AGG | - | chr3.1:92859015-92859034 | None:intergenic | 20.0% |
| !! | TTCAACTTAATAATTGCATT+GGG | - | chr3.1:92859103-92859122 | None:intergenic | 20.0% |
| !! | TTTCAACTTAATAATTGCAT+TGG | - | chr3.1:92859104-92859123 | None:intergenic | 20.0% |
| !!! | CAATTATTAAGTTGAAAATG+TGG | + | chr3.1:92859107-92859126 | MS.gene68134:intron | 20.0% |
| !!! | CTTTACTCTACTTTTATTTT+AGG | + | chr3.1:92858897-92858916 | MS.gene68134:intron | 20.0% |
| !!! | TCTACTTTCTTGTTTTTTTA+GGG | + | chr3.1:92859053-92859072 | MS.gene68134:intron | 20.0% |
| ! | CTAAAAAAACAAGAAAGTAG+AGG | - | chr3.1:92859054-92859073 | None:intergenic | 25.0% |
| ! | TCAACTTAATAATTGCATTG+GGG | - | chr3.1:92859102-92859121 | None:intergenic | 25.0% |
| !! | GTTTGTTACTTAGTTAGTTA+GGG | + | chr3.1:92859021-92859040 | MS.gene68134:intron | 25.0% |
| !! | TGTTTGTTACTTAGTTAGTT+AGG | + | chr3.1:92859020-92859039 | MS.gene68134:intron | 25.0% |
| !!! | CTCTACTTTCTTGTTTTTTT+AGG | + | chr3.1:92859052-92859071 | MS.gene68134:intron | 25.0% |
| !!! | TTCTTGTTTTTTTAGGGTTT+AGG | + | chr3.1:92859059-92859078 | MS.gene68134:intron | 25.0% |
| AAAACAAGAAAGTAGAGGAT+TGG | - | chr3.1:92859049-92859068 | None:intergenic | 30.0% | |
| AACTTAATAATTGCATTGGG+GGG | - | chr3.1:92859100-92859119 | None:intergenic | 30.0% | |
| ACATCTTATCTGATAAACCA+AGG | + | chr3.1:92859465-92859484 | MS.gene68134:CDS | 30.0% | |
| ATTCGTGAAGAAGATATCAT+TGG | + | chr3.1:92859302-92859321 | MS.gene68134:CDS | 30.0% | |
| CAACTTAATAATTGCATTGG+GGG | - | chr3.1:92859101-92859120 | None:intergenic | 30.0% | |
| CATCTTATCTGATAAACCAA+GGG | + | chr3.1:92859466-92859485 | MS.gene68134:CDS | 30.0% | |
| ! | CTTCATTTTCATTGTTCTCA+GGG | - | chr3.1:92859517-92859536 | None:intergenic | 30.0% |
| ! | TCTTCATTTTCATTGTTCTC+AGG | - | chr3.1:92859518-92859537 | None:intergenic | 30.0% |
| ! | TTTTAACAGATAGGGTTCAA+AGG | + | chr3.1:92859441-92859460 | MS.gene68134:CDS | 30.0% |
| !! | AACCAAGGTTTTAACAGATA+GGG | + | chr3.1:92859433-92859452 | MS.gene68134:CDS | 30.0% |
| !!! | AAACTATCGCTTTGTTTTAC+AGG | + | chr3.1:92859256-92859275 | MS.gene68134:CDS | 30.0% |
| AACCCTATCTGTTAAAACCT+TGG | - | chr3.1:92859438-92859457 | None:intergenic | 35.0% | |
| ATAGGAAATCTCGAACTGAA+AGG | - | chr3.1:92858837-92858856 | None:intergenic | 35.0% | |
| ATGGTCAATTGTCACAGATT+TGG | + | chr3.1:92859145-92859164 | MS.gene68134:intron | 35.0% | |
| CAATTGTCACAGATTTGGAA+TGG | + | chr3.1:92859150-92859169 | MS.gene68134:intron | 35.0% | |
| CTATCTGTTAAAACCTTGGT+TGG | - | chr3.1:92859434-92859453 | None:intergenic | 35.0% | |
| GATATCTTCTTCACGAATCT+TGG | - | chr3.1:92859300-92859319 | None:intergenic | 35.0% | |
| TAGGAAATCTCGAACTGAAA+GGG | - | chr3.1:92858836-92858855 | None:intergenic | 35.0% | |
| TCTACTCACATGCTCTTATT+TGG | + | chr3.1:92859337-92859356 | MS.gene68134:CDS | 35.0% | |
| TGAGACTTATGACCAGTTAT+TGG | + | chr3.1:92859202-92859221 | MS.gene68134:CDS | 35.0% | |
| ! | ATACATACCATCTTTGAGAG+CGG | - | chr3.1:92858870-92858889 | None:intergenic | 35.0% |
| ! | ATTTTCGTTTTCACTCTGCT+AGG | + | chr3.1:92858938-92858957 | MS.gene68134:intron | 35.0% |
| ! | CTACTCACATGCTCTTATTT+GGG | + | chr3.1:92859338-92859357 | MS.gene68134:CDS | 35.0% |
| ! | GAATGGAAGCTGATTTATGT+TGG | + | chr3.1:92859167-92859186 | MS.gene68134:CDS | 35.0% |
| ! | GTGGACTATCAATTCATTCA+TGG | + | chr3.1:92859126-92859145 | MS.gene68134:intron | 35.0% |
| !! | CAACCAAGGTTTTAACAGAT+AGG | + | chr3.1:92859432-92859451 | MS.gene68134:CDS | 35.0% |
| CGAGATTTCCTATGAGTGTT+TGG | + | chr3.1:92858844-92858863 | MS.gene68134:CDS | 40.0% | |
| CGATAGTTTCCAACATTGAC+AGG | - | chr3.1:92859245-92859264 | None:intergenic | 40.0% | |
| CTTCTTCACGAATCTTGGAT+GGG | - | chr3.1:92859295-92859314 | None:intergenic | 40.0% | |
| GATTTATGTTGGATCTGCTG+AGG | + | chr3.1:92859178-92859197 | MS.gene68134:CDS | 40.0% | |
| GGTCAAGAGTTTATTCGTGT+TGG | + | chr3.1:92859359-92859378 | MS.gene68134:CDS | 40.0% | |
| TAATAATTGCATTGGGGGGA+GGG | - | chr3.1:92859096-92859115 | None:intergenic | 40.0% | |
| TCTGTTAAAACCTTGGTTGG+AGG | - | chr3.1:92859431-92859450 | None:intergenic | 40.0% | |
| TCTTCTTCACGAATCTTGGA+TGG | - | chr3.1:92859296-92859315 | None:intergenic | 40.0% | |
| TGGATCTTGTTCTCCAGTTT+GGG | - | chr3.1:92859567-92859586 | None:intergenic | 40.0% | |
| TTAATAATTGCATTGGGGGG+AGG | - | chr3.1:92859097-92859116 | None:intergenic | 40.0% | |
| TTCTCAGGGTGGAAATTGAT+GGG | - | chr3.1:92859503-92859522 | None:intergenic | 40.0% | |
| ! | CATTTTCATTGTTCTCAGGG+TGG | - | chr3.1:92859514-92859533 | None:intergenic | 40.0% |
| ! | GTTTTCACTCTGCTAGGTTA+GGG | + | chr3.1:92858944-92858963 | MS.gene68134:intron | 40.0% |
| !! | TTATTGGAGAGTGTCCTTGT+TGG | + | chr3.1:92859218-92859237 | MS.gene68134:CDS | 40.0% |
| AAGGGATTGAGAAACGAAGC+TGG | - | chr3.1:92858818-92858837 | None:intergenic | 45.0% | |
| AATAATTGCATTGGGGGGAG+GGG | - | chr3.1:92859095-92859114 | None:intergenic | 45.0% | |
| ACTCTGCTAGGTTAGGGTTT+AGG | + | chr3.1:92858950-92858969 | MS.gene68134:intron | 45.0% | |
| ATACCATTCCCCCAAATGAG+GGG | + | chr3.1:92859603-92859622 | MS.gene68134:CDS | 45.0% | |
| CAACATTGACAGGACCAACA+AGG | - | chr3.1:92859235-92859254 | None:intergenic | 45.0% | |
| CAAGGACACTCTCCAATAAC+TGG | - | chr3.1:92859217-92859236 | None:intergenic | 45.0% | |
| CTCATTTGGGGGAATGGTAT+CGG | - | chr3.1:92859603-92859622 | None:intergenic | 45.0% | |
| CTCTGCTAGGTTAGGGTTTA+GGG | + | chr3.1:92858951-92858970 | MS.gene68134:intron | 45.0% | |
| CTTGTTGGTCCTGTCAATGT+TGG | + | chr3.1:92859233-92859252 | MS.gene68134:CDS | 45.0% | |
| GAAACGAAGCTGGATTGTCA+AGG | - | chr3.1:92858808-92858827 | None:intergenic | 45.0% | |
| GATACCATTCCCCCAAATGA+GGG | + | chr3.1:92859602-92859621 | MS.gene68134:CDS | 45.0% | |
| GGATCTTGTTCTCCAGTTTG+GGG | - | chr3.1:92859566-92859585 | None:intergenic | 45.0% | |
| GGTTAACATCACGAACGTGA+CGG | + | chr3.1:92858781-92858800 | MS.gene68134:CDS | 45.0% | |
| GTGGATCTTGTTCTCCAGTT+TGG | - | chr3.1:92859568-92859587 | None:intergenic | 45.0% | |
| GTTCTCAGGGTGGAAATTGA+TGG | - | chr3.1:92859504-92859523 | None:intergenic | 45.0% | |
| TCATTTGGGGGAATGGTATC+GGG | - | chr3.1:92859602-92859621 | None:intergenic | 45.0% | |
| TCTCAGGGTGGAAATTGATG+GGG | - | chr3.1:92859502-92859521 | None:intergenic | 45.0% | |
| TTAAGAACCCCCCTCATTTG+GGG | - | chr3.1:92859615-92859634 | None:intergenic | 45.0% | |
| ! | CGTTTTCACTCTGCTAGGTT+AGG | + | chr3.1:92858943-92858962 | MS.gene68134:intron | 45.0% |
| ATGGGGAACTTAGTGACCCT+TGG | - | chr3.1:92859485-92859504 | None:intergenic | 50.0% | |
| CGATACCATTCCCCCAAATG+AGG | + | chr3.1:92859601-92859620 | MS.gene68134:CDS | 50.0% | |
| TAAGAACCCCCCTCATTTGG+GGG | - | chr3.1:92859614-92859633 | None:intergenic | 50.0% | |
| TACCATTCCCCCAAATGAGG+GGG | + | chr3.1:92859604-92859623 | MS.gene68134:CDS | 50.0% | |
| TCACGAATCTTGGATGGGTC+TGG | - | chr3.1:92859290-92859309 | None:intergenic | 50.0% | |
| TCAGAGCAACTACCCCAAAC+TGG | + | chr3.1:92859551-92859570 | MS.gene68134:CDS | 50.0% | |
| TGTTTGGCCGCTCTCAAAGA+TGG | + | chr3.1:92858860-92858879 | MS.gene68134:CDS | 50.0% | |
| ! | AGTTTGGGGTAGTTGCTCTG+AGG | - | chr3.1:92859552-92859571 | None:intergenic | 50.0% |
| ! | GTTTGGGGTAGTTGCTCTGA+GGG | - | chr3.1:92859551-92859570 | None:intergenic | 50.0% |
| ACCATTCCCCCAAATGAGGG+GGG | + | chr3.1:92859605-92859624 | MS.gene68134:CDS | 55.0% | |
| CGAATCTTGGATGGGTCTGG+TGG | - | chr3.1:92859287-92859306 | None:intergenic | 55.0% | |
| GAGAGAGGAACCTCCAACCA+AGG | + | chr3.1:92859418-92859437 | MS.gene68134:CDS | 55.0% | |
| GAGAGCGGCCAAACACTCAT+AGG | - | chr3.1:92858855-92858874 | None:intergenic | 55.0% | |
| ACCCCCCTCATTTGGGGGAA+TGG | - | chr3.1:92859609-92859628 | None:intergenic | 60.0% | |
| GTATCGGGTGCAGCAGCAAG+TGG | - | chr3.1:92859587-92859606 | None:intergenic | 60.0% | |
| TGACGACGAGCAGCTGAGAG+AGG | + | chr3.1:92859403-92859422 | MS.gene68134:CDS | 60.0% | |
| !! | GGGGTAGTTGCTCTGAGGGA+GGG | - | chr3.1:92859547-92859566 | None:intergenic | 60.0% |
| !! | GGGTAGTTGCTCTGAGGGAG+GGG | - | chr3.1:92859546-92859565 | None:intergenic | 60.0% |
| !! | GGTAGTTGCTCTGAGGGAGG+GGG | - | chr3.1:92859545-92859564 | None:intergenic | 60.0% |
| !! | TGGGGTAGTTGCTCTGAGGG+AGG | - | chr3.1:92859548-92859567 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 92858773 | 92859634 | 92858773 | ID=MS.gene68134 |
| chr3.1 | mRNA | 92858773 | 92859634 | 92858773 | ID=MS.gene68134.t1;Parent=MS.gene68134 |
| chr3.1 | exon | 92858773 | 92858881 | 92858773 | ID=MS.gene68134.t1.exon1;Parent=MS.gene68134.t1 |
| chr3.1 | CDS | 92858773 | 92858881 | 92858773 | ID=cds.MS.gene68134.t1;Parent=MS.gene68134.t1 |
| chr3.1 | exon | 92859162 | 92859634 | 92859162 | ID=MS.gene68134.t1.exon2;Parent=MS.gene68134.t1 |
| chr3.1 | CDS | 92859162 | 92859634 | 92859162 | ID=cds.MS.gene68134.t1;Parent=MS.gene68134.t1 |
| Gene Sequence |
| Protein sequence |
