AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066035


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066035.t1 MTR_1g075720 99.248 133 1 0 1 133 1 133 2.34e-90 258
MS.gene066035.t1 MTR_4g120770 95.489 133 6 0 1 133 1 133 4.44e-89 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066035.t1 AT2G20450 84.962 133 20 0 1 133 1 133 7.52e-77 224
MS.gene066035.t1 AT4G27090 85.385 130 19 0 1 130 1 130 6.52e-76 221

Find 38 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACCTATCAAAATCATTA+AGG 0.237759 1.2:-58370588 None:intergenic
TTGACGAGATCCTTCTTCTT+AGG 0.248112 1.2:-58370363 None:intergenic
GATTATGTTGGCCAAGATTA+AGG 0.263288 1.2:+58370612 MS.gene066035:CDS
GTCAACTATGGTCCAGATTA+TGG 0.285686 1.2:+58370115 MS.gene066035:CDS
GAAAATGGGTTACCCTAGTT+TGG 0.329740 1.2:-58370168 None:intergenic
GAAAGCCTCTTGAAATTGAT+TGG 0.348542 1.2:-58370306 None:intergenic
ACAACAAGCTTGCCATAATC+TGG 0.353003 1.2:-58370127 None:intergenic
CTAAAATTATACCTTAATCT+TGG 0.381940 1.2:-58370623 None:intergenic
TGCAGAGGGCTGGTGTTGTT+AGG 0.431787 1.2:+58371341 MS.gene066035:intron
TTGATGTTATCGACCAAACT+AGG 0.433748 1.2:+58370155 MS.gene066035:CDS
GAACCTATCAAAATCATTAA+GGG 0.440003 1.2:-58370587 None:intergenic
AACAATCAATTTCCTTCCCC+AGG 0.448020 1.2:-58370548 None:intergenic
ATTGGAGCTCTCTCCATGTC+AGG 0.463357 1.2:-58370288 None:intergenic
AGAGTTGCACTTGTCAACTA+TGG 0.467898 1.2:+58370103 MS.gene066035:CDS
AATTCTACCTGCAGCTTCCA+TGG 0.474519 1.2:-58370388 None:intergenic
GTTGAGGAAGACCGCAGCTT+AGG 0.482197 1.2:+58371378 MS.gene066035:CDS
TTTCAATTTGTGCAGAGGGC+TGG 0.483986 1.2:+58371331 MS.gene066035:intron
GGAAATTGATTGTTAAGAAG+AGG 0.493248 1.2:+58370557 MS.gene066035:CDS
TTCAAGAGGTTTGTTGAGAT+CGG 0.494020 1.2:+58370079 MS.gene066035:CDS
CAAGAGAGTTCCTAAGAAGA+AGG 0.504705 1.2:+58370353 MS.gene066035:CDS
GGCAAGAACTTGCTAAGTTG+AGG 0.522233 1.2:+58371362 MS.gene066035:CDS
ACAAGTGGGAGAAGAGCTCC+TGG 0.539704 1.2:+58370530 MS.gene066035:CDS
AATTGATTGTTAAGAAGAGG+AGG 0.539799 1.2:+58370560 MS.gene066035:CDS
TGATAGGTTCAAGATTATGT+TGG 0.542816 1.2:+58370600 MS.gene066035:CDS
TGATAGATGTGAAGAACAAG+TGG 0.562094 1.2:+58370515 MS.gene066035:intron
GTCAAAGCCATGGAAGCTGC+AGG 0.563654 1.2:+58370381 MS.gene066035:CDS
ATCTCAACAAACCTCTTGAA+CGG 0.571035 1.2:-58370076 None:intergenic
GAAGGATCTCGTCAAAGCCA+TGG 0.572972 1.2:+58370371 MS.gene066035:CDS
GAGCTCCAATCAATTTCAAG+AGG 0.584468 1.2:+58370301 MS.gene066035:CDS
GGGAGAAGAGCTCCTGGGGA+AGG 0.586483 1.2:+58370536 MS.gene066035:CDS
TCTTTGTTCAGCCGTTCAAG+AGG 0.591089 1.2:+58370065 MS.gene066035:intron
TCTTGTTGATGCACCTGACA+TGG 0.596389 1.2:+58370275 MS.gene066035:CDS
ATTGATTGTTAAGAAGAGGA+GGG 0.602431 1.2:+58370561 MS.gene066035:CDS
TGATGTTATCGACCAAACTA+GGG 0.607546 1.2:+58370156 MS.gene066035:CDS
AAGTGGGAGAAGAGCTCCTG+GGG 0.619297 1.2:+58370532 MS.gene066035:CDS
GATAGATGTGAAGAACAAGT+GGG 0.623335 1.2:+58370516 MS.gene066035:intron
CAAGTGGGAGAAGAGCTCCT+GGG 0.641380 1.2:+58370531 MS.gene066035:CDS
ATGAAGTAATGCCTAAGCTG+CGG 0.676586 1.2:-58371389 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGATTTTTTTTTTTTTG+TGG + chr1.2:58370472-58370491 MS.gene066035:intron 10.0%
!! ACAAAAATAAATACATAGAT+AGG - chr1.2:58370425-58370444 None:intergenic 15.0%
!!! TTTTTTTTTTTTGTGGTAAT+TGG + chr1.2:58370479-58370498 MS.gene066035:intron 15.0%
!! AAAGATTGGAAATTTGAAAA+TGG - chr1.2:58370186-58370205 None:intergenic 20.0%
!! AAGATTGGAAATTTGAAAAT+GGG - chr1.2:58370185-58370204 None:intergenic 20.0%
!! ATTAGAGAAAGATTAAGAAA+TGG - chr1.2:58370033-58370052 None:intergenic 20.0%
!! CTAAAATTATACCTTAATCT+TGG - chr1.2:58370626-58370645 None:intergenic 20.0%
!! TTAAGTTATTTATCCAAACA+AGG - chr1.2:58370777-58370796 None:intergenic 20.0%
!!! ATTGTATTTCTATAAGCTTT+AGG + chr1.2:58370880-58370899 MS.gene066035:intron 20.0%
!!! TTGTATTTCTATAAGCTTTA+GGG + chr1.2:58370881-58370900 MS.gene066035:intron 20.0%
!!! TTTTCAGAAATTAAGCAAAT+GGG - chr1.2:58370226-58370245 None:intergenic 20.0%
!!! TTTTTCAGAAATTAAGCAAA+TGG - chr1.2:58370227-58370246 None:intergenic 20.0%
! CAAGTTTGTCTATGAAAAAA+CGG - chr1.2:58371004-58371023 None:intergenic 25.0%
! GAACCTATCAAAATCATTAA+GGG - chr1.2:58370590-58370609 None:intergenic 25.0%
! GGTTATTATATAAGCTGTTA+TGG + chr1.2:58370820-58370839 MS.gene066035:intron 25.0%
! TAAAATAAGCTAATCCAAAC+AGG + chr1.2:58371215-58371234 MS.gene066035:intron 25.0%
! TAAAATATTAAGGGTCTGTT+TGG - chr1.2:58371088-58371107 None:intergenic 25.0%
! TAAATTTCAATTTGTGCAGA+GGG + chr1.2:58371327-58371346 MS.gene066035:intron 25.0%
! TGAACCTATCAAAATCATTA+AGG - chr1.2:58370591-58370610 None:intergenic 25.0%
! TTAAATTTCAATTTGTGCAG+AGG + chr1.2:58371326-58371345 MS.gene066035:intron 25.0%
!! TTGCATTTTATAGTGTAAGT+GGG + chr1.2:58370798-58370817 MS.gene066035:intron 25.0%
!! TTTGCATTTTATAGTGTAAG+TGG + chr1.2:58370797-58370816 MS.gene066035:intron 25.0%
!!! TGTGAAATGATTTTAAGTCA+GGG + chr1.2:58370667-58370686 MS.gene066035:intron 25.0%
!!! TTGTGAAATGATTTTAAGTC+AGG + chr1.2:58370666-58370685 MS.gene066035:intron 25.0%
!!! TTGTTTTTCAAATTGTGTCT+AGG + chr1.2:58371266-58371285 MS.gene066035:intron 25.0%
AATTGATTGTTAAGAAGAGG+AGG + chr1.2:58370560-58370579 MS.gene066035:CDS 30.0%
ATGGAGATACAATGTTTGTT+AGG - chr1.2:58370978-58370997 None:intergenic 30.0%
ATTGATTGTTAAGAAGAGGA+GGG + chr1.2:58370561-58370580 MS.gene066035:CDS 30.0%
GGAAATTGATTGTTAAGAAG+AGG + chr1.2:58370557-58370576 MS.gene066035:CDS 30.0%
GGATGAAAATGGAAAAAGAT+TGG - chr1.2:58370200-58370219 None:intergenic 30.0%
TGATAGGTTCAAGATTATGT+TGG + chr1.2:58370600-58370619 MS.gene066035:CDS 30.0%
TTGTGAAATGCTCTTAAGTT+GGG + chr1.2:58370751-58370770 MS.gene066035:intron 30.0%
! TGCATTTTATAGTGTAAGTG+GGG + chr1.2:58370799-58370818 MS.gene066035:intron 30.0%
! TGGAGATACAATGTTTGTTA+GGG - chr1.2:58370977-58370996 None:intergenic 30.0%
!!! TTGTGATTTGTTTGTTTTGC+AGG + chr1.2:58370251-58370270 MS.gene066035:intron 30.0%
AAACCCTACATACTCAACTA+AGG - chr1.2:58371242-58371261 None:intergenic 35.0%
AACCCTACATACTCAACTAA+GGG - chr1.2:58371241-58371260 None:intergenic 35.0%
ATCTCAACAAACCTCTTGAA+CGG - chr1.2:58370079-58370098 None:intergenic 35.0%
GAAAGCCTCTTGAAATTGAT+TGG - chr1.2:58370309-58370328 None:intergenic 35.0%
GATAGATGTGAAGAACAAGT+GGG + chr1.2:58370516-58370535 MS.gene066035:intron 35.0%
GATTATGTTGGCCAAGATTA+AGG + chr1.2:58370612-58370631 MS.gene066035:CDS 35.0%
GTCTATGAAAAAACGGCTTA+TGG - chr1.2:58370997-58371016 None:intergenic 35.0%
GTTGTGAAATGCTCTTAAGT+TGG + chr1.2:58370750-58370769 MS.gene066035:intron 35.0%
TATTAAGGGTCTGTTTGGAT+TGG - chr1.2:58371083-58371102 None:intergenic 35.0%
TGATAGATGTGAAGAACAAG+TGG + chr1.2:58370515-58370534 MS.gene066035:intron 35.0%
TGATGTTATCGACCAAACTA+GGG + chr1.2:58370156-58370175 MS.gene066035:CDS 35.0%
TTCTCATAACCCAATTCAAG+CGG - chr1.2:58370908-58370927 None:intergenic 35.0%
TTGATGTTATCGACCAAACT+AGG + chr1.2:58370155-58370174 MS.gene066035:CDS 35.0%
! AGAAATTAAGCAAATGGGCT+AGG - chr1.2:58370221-58370240 None:intergenic 35.0%
! ATTAAGTTGTCTATCCAAGC+AGG - chr1.2:58370694-58370713 None:intergenic 35.0%
! TTAAGTTGTCTATCCAAGCA+GGG - chr1.2:58370693-58370712 None:intergenic 35.0%
!! CTGCCCTTAATGATTTTGAT+AGG + chr1.2:58370584-58370603 MS.gene066035:CDS 35.0%
!! TTCAAGAGGTTTGTTGAGAT+CGG + chr1.2:58370079-58370098 MS.gene066035:CDS 35.0%
!!! TTTGGATTGGCTTCTTTTTG+AGG - chr1.2:58371070-58371089 None:intergenic 35.0%
AACAATCAATTTCCTTCCCC+AGG - chr1.2:58370551-58370570 None:intergenic 40.0%
ACAACAAGCTTGCCATAATC+TGG - chr1.2:58370130-58370149 None:intergenic 40.0%
AGAGTTGCACTTGTCAACTA+TGG + chr1.2:58370103-58370122 MS.gene066035:CDS 40.0%
CAAGAGAGTTCCTAAGAAGA+AGG + chr1.2:58370353-58370372 MS.gene066035:CDS 40.0%
GAGCTCCAATCAATTTCAAG+AGG + chr1.2:58370301-58370320 MS.gene066035:CDS 40.0%
GTCAACTATGGTCCAGATTA+TGG + chr1.2:58370115-58370134 MS.gene066035:CDS 40.0%
TCTCATAACCCAATTCAAGC+GGG - chr1.2:58370907-58370926 None:intergenic 40.0%
TGTCTGTGAGTACTTATGTG+TGG - chr1.2:58371166-58371185 None:intergenic 40.0%
TTGACGAGATCCTTCTTCTT+AGG - chr1.2:58370366-58370385 None:intergenic 40.0%
TTTGCAGCTTGTAGTTCAAG+TGG + chr1.2:58370713-58370732 MS.gene066035:intron 40.0%
!! CAAATGGGCTAGGATGAAAA+TGG - chr1.2:58370211-58370230 None:intergenic 40.0%
!! GAAAATGGGTTACCCTAGTT+TGG - chr1.2:58370171-58370190 None:intergenic 40.0%
AATTCTACCTGCAGCTTCCA+TGG - chr1.2:58370391-58370410 None:intergenic 45.0%
AGGCCCTTAGTTGAGTATGT+AGG + chr1.2:58371235-58371254 MS.gene066035:intron 45.0%
GGCCCTTAGTTGAGTATGTA+GGG + chr1.2:58371236-58371255 MS.gene066035:intron 45.0%
TACTCAACTAAGGGCCTGTT+TGG - chr1.2:58371232-58371251 None:intergenic 45.0%
TCTTGTTGATGCACCTGACA+TGG + chr1.2:58370275-58370294 MS.gene066035:CDS 45.0%
TCTTTGTTCAGCCGTTCAAG+AGG + chr1.2:58370065-58370084 MS.gene066035:intron 45.0%
TTTCAATTTGTGCAGAGGGC+TGG + chr1.2:58371331-58371350 MS.gene066035:intron 45.0%
! CAGACAGTCTTGCATGCTTT+TGG + chr1.2:58371180-58371199 MS.gene066035:intron 45.0%
!! CTCTTAAGTTGGGCCTTGTT+TGG + chr1.2:58370761-58370780 MS.gene066035:intron 45.0%
!! GGCAAGAACTTGCTAAGTTG+AGG + chr1.2:58371362-58371381 MS.gene066035:CDS 45.0%
!!! AATTTTATGATAAAATATTA+AGG - chr1.2:58371098-58371117 None:intergenic 5.0%
!!! ATTTTATGATAAAATATTAA+GGG - chr1.2:58371097-58371116 None:intergenic 5.0%
!!! TAATATTTTATCATAAAATT+TGG + chr1.2:58371098-58371117 MS.gene066035:intron 5.0%
ATTGGAGCTCTCTCCATGTC+AGG - chr1.2:58370291-58370310 None:intergenic 50.0%
GAAGGATCTCGTCAAAGCCA+TGG + chr1.2:58370371-58370390 MS.gene066035:CDS 50.0%
!! CTTTAGGGCCCGCTTGAATT+GGG + chr1.2:58370896-58370915 MS.gene066035:intron 50.0%
!! TTTTAAGTCAGGGCCCTGCT+TGG + chr1.2:58370677-58370696 MS.gene066035:intron 50.0%
AAGTGGGAGAAGAGCTCCTG+GGG + chr1.2:58370532-58370551 MS.gene066035:CDS 55.0%
ACAAGTGGGAGAAGAGCTCC+TGG + chr1.2:58370530-58370549 MS.gene066035:CDS 55.0%
CAAGTGGGAGAAGAGCTCCT+GGG + chr1.2:58370531-58370550 MS.gene066035:CDS 55.0%
GTCAAAGCCATGGAAGCTGC+AGG + chr1.2:58370381-58370400 MS.gene066035:CDS 55.0%
!! GCTTTAGGGCCCGCTTGAAT+TGG + chr1.2:58370895-58370914 MS.gene066035:intron 55.0%
!! TGCAGAGGGCTGGTGTTGTT+AGG + chr1.2:58371341-58371360 MS.gene066035:intron 55.0%
GGGAGAAGAGCTCCTGGGGA+AGG + chr1.2:58370536-58370555 MS.gene066035:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 58369991 58371399 58369991 ID=MS.gene066035
chr1.2 mRNA 58369991 58371399 58369991 ID=MS.gene066035.t1;Parent=MS.gene066035
chr1.2 exon 58369991 58369993 58369991 ID=MS.gene066035.t1.exon1;Parent=MS.gene066035.t1
chr1.2 CDS 58369991 58369993 58369991 ID=cds.MS.gene066035.t1;Parent=MS.gene066035.t1
chr1.2 exon 58370076 58370177 58370076 ID=MS.gene066035.t1.exon2;Parent=MS.gene066035.t1
chr1.2 CDS 58370076 58370177 58370076 ID=cds.MS.gene066035.t1;Parent=MS.gene066035.t1
chr1.2 exon 58370273 58370402 58370273 ID=MS.gene066035.t1.exon3;Parent=MS.gene066035.t1
chr1.2 CDS 58370273 58370402 58370273 ID=cds.MS.gene066035.t1;Parent=MS.gene066035.t1
chr1.2 exon 58370521 58370633 58370521 ID=MS.gene066035.t1.exon4;Parent=MS.gene066035.t1
chr1.2 CDS 58370521 58370633 58370521 ID=cds.MS.gene066035.t1;Parent=MS.gene066035.t1
chr1.2 exon 58371346 58371399 58371346 ID=MS.gene066035.t1.exon5;Parent=MS.gene066035.t1
chr1.2 CDS 58371346 58371399 58371346 ID=cds.MS.gene066035.t1;Parent=MS.gene066035.t1
Gene Sequence

>MS.gene066035

ATGGTGAATCACATTTCTCTGTTCATCTTTGTCTCAATTCCATTTCTTAATCTTTCTCTAATCTCACTGTGTGTTCTTTGTTCAGCCGTTCAAGAGGTTTGTTGAGATCGGAAGAGTTGCACTTGTCAACTATGGTCCAGATTATGGCAAGCTTGTTGTTATCGTTGATGTTATCGACCAAACTAGGGTAACCCATTTTCAAATTTCCAATCTTTTTCCATTTTCATCCTAGCCCATTTGCTTAATTTCTGAAAAATGTTTTGTGATTTGTTTGTTTTGCAGGCTCTTGTTGATGCACCTGACATGGAGAGAGCTCCAATCAATTTCAAGAGGCTTTCTCTAACTGATATCAAGATTGATATCAAGAGAGTTCCTAAGAAGAAGGATCTCGTCAAAGCCATGGAAGCTGCAGGTAGAATTTCTTCTATTAACCTATCTATGTATTTATTTTTGTTTTTGTTTTTATATTTTGTTTGTTGAAATTTGATTTTTTTTTTTTTGTGGTAATTGGTTTTGTTTGAATTTGATAGATGTGAAGAACAAGTGGGAGAAGAGCTCCTGGGGAAGGAAATTGATTGTTAAGAAGAGGAGGGCTGCCCTTAATGATTTTGATAGGTTCAAGATTATGTTGGCCAAGATTAAGGTATAATTTTAGTTGCTGTTTTTTGTTTTCTGTTGTGAAATGATTTTAAGTCAGGGCCCTGCTTGGATAGACAACTTAATTTGCAGCTTGTAGTTCAAGTGGCTATTATAGAAGCTGTTGTGAAATGCTCTTAAGTTGGGCCTTGTTTGGATAAATAACTTAATTTGCATTTTATAGTGTAAGTGGGGTTATTATATAAGCTGTTATGGATGAACTGTTTCTGTTGCACAAGATAAGATGAATCAAATTGTATTTCTATAAGCTTTAGGGCCCGCTTGAATTGGGTTATGAGAAGTAGCTTATGTAAATAAATAAGCTCTTATGTTAATTCATAAGTTCTCCCTAACAAACATTGTATCTCCATAAGCCGTTTTTTCATAGACAAACTTGACAAGCTCATGAATAATACATAAAAGCTTATTTATTTGAATAACCTCAAAAAGAAGCCAATCCAAACAGACCCTTAATATTTTATCATAAAATTTGGAGAACTTATCAAATGAAACTGTTAAAACAACTTTTGAACATGCCACACATAAGTACTCACAGACAGTCTTGCATGCTTTTGGCTGTTTATAAGCTAAAATAAGCTAATCCAAACAGGCCCTTAGTTGAGTATGTAGGGTTTGATTTTGTTTTTCAAATTGTGTCTAGGTTGTATTAATTCTGATTCTGATGTTGTATTGATGTTCTTAAATTTCAATTTGTGCAGAGGGCTGGTGTTGTTAGGCAAGAACTTGCTAAGTTGAGGAAGACCGCAGCTTAG

Protein sequence

>MS.gene066035.t1

MPFKRFVEIGRVALVNYGPDYGKLVVIVDVIDQTRALVDAPDMERAPINFKRLSLTDIKIDIKRVPKKKDLVKAMEAADVKNKWEKSSWGRKLIVKKRRAALNDFDRFKIMLAKIKRAGVVRQELAKLRKTAA