Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063084.t1 | XP_014499992.1 | 95.6 | 180 | 7 | 1 | 1 | 180 | 1 | 179 | 6.00E-91 | 343.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063084.t1 | Q9LS09 | 86.4 | 169 | 22 | 1 | 1 | 168 | 1 | 169 | 3.1e-80 | 299.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063084.t1 | A0A1S3U1V4 | 95.6 | 180 | 7 | 1 | 1 | 180 | 1 | 179 | 4.3e-91 | 343.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene063084 | MS.gene066035 | PPI |
| MS.gene66836 | MS.gene063084 | PPI |
| MS.gene016712 | MS.gene063084 | PPI |
| MS.gene063084 | MS.gene004833 | PPI |
| MS.gene94315 | MS.gene063084 | PPI |
| MS.gene71415 | MS.gene063084 | PPI |
| MS.gene064876 | MS.gene063084 | PPI |
| MS.gene063084 | MS.gene041630 | PPI |
| MS.gene063084 | MS.gene81646 | PPI |
| MS.gene066034 | MS.gene063084 | PPI |
| MS.gene063084 | MS.gene069975 | PPI |
| MS.gene94307 | MS.gene063084 | PPI |
| MS.gene070538 | MS.gene063084 | PPI |
| MS.gene98552 | MS.gene063084 | PPI |
| MS.gene52972 | MS.gene063084 | PPI |
| MS.gene52970 | MS.gene063084 | PPI |
| MS.gene014301 | MS.gene063084 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063084.t1 | MTR_3g118150 | 95.855 | 193 | 8 | 0 | 1 | 193 | 1 | 193 | 1.58e-134 | 374 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063084.t1 | AT5G38110 | 86.391 | 169 | 22 | 1 | 1 | 168 | 1 | 169 | 1.31e-104 | 300 |
| MS.gene063084.t1 | AT1G66740 | 80.899 | 178 | 31 | 1 | 1 | 178 | 1 | 175 | 2.90e-103 | 295 |
Find 53 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTACTCACCTGCTCTTATT+TGG | 0.129518 | 3.3:+95872481 | MS.gene063084:CDS |
| TGGATCTTGTTCTCCAGTTT+GGG | 0.231870 | 3.3:-95872708 | None:intergenic |
| GTGGATCTTGTTCTCCAGTT+TGG | 0.266545 | 3.3:-95872709 | None:intergenic |
| CTACTCACCTGCTCTTATTT+GGG | 0.267855 | 3.3:+95872482 | MS.gene063084:CDS |
| GATATCTTCTTCACGAATCT+TGG | 0.287365 | 3.3:-95872441 | None:intergenic |
| TGAGACTTATGACCAGTTAT+TGG | 0.289525 | 3.3:+95872346 | MS.gene063084:CDS |
| CGAGATTTCCTATGAGTGTT+TGG | 0.353611 | 3.3:+95872004 | MS.gene063084:CDS |
| GGGTAGTTGCTCTGAGGGAG+GGG | 0.371206 | 3.3:-95872687 | None:intergenic |
| TGGGGTAGTTGCTCTGAGGG+AGG | 0.378053 | 3.3:-95872689 | None:intergenic |
| GGGGTAGTTGCTCTGAGGGA+GGG | 0.392562 | 3.3:-95872688 | None:intergenic |
| GTTCTCAGGGTGGAAATTGA+TGG | 0.403676 | 3.3:-95872645 | None:intergenic |
| GGTAGTTGCTCTGAGGGAGG+GGG | 0.407080 | 3.3:-95872686 | None:intergenic |
| CAAGGACACTCTCCAATAAC+TGG | 0.417188 | 3.3:-95872358 | None:intergenic |
| TCACGAATCTTGGATGGGTC+TGG | 0.425679 | 3.3:-95872431 | None:intergenic |
| TCAGAGCAACTACCCCAAAC+TGG | 0.436456 | 3.3:+95872695 | MS.gene063084:CDS |
| GAGAGCGGCCAAACACTCAT+AGG | 0.445137 | 3.3:-95872012 | None:intergenic |
| CTATCTGTTAAAACCTTGGT+TGG | 0.449270 | 3.3:-95872575 | None:intergenic |
| AACCCTATCTGTTAAAACCT+TGG | 0.452925 | 3.3:-95872579 | None:intergenic |
| TTATTGGAGAGTGTCCTTGT+TGG | 0.475012 | 3.3:+95872362 | MS.gene063084:CDS |
| TTCTCAGGGTGGAAATTGAT+GGG | 0.479943 | 3.3:-95872644 | None:intergenic |
| TCTGTTAAAACCTTGGTTGG+AGG | 0.483580 | 3.3:-95872572 | None:intergenic |
| GAATGGAAGCTGATTTATGT+TGG | 0.485287 | 3.3:+95872311 | MS.gene063084:CDS |
| TCTTCTTCACGAATCTTGGA+TGG | 0.488809 | 3.3:-95872437 | None:intergenic |
| ATGGGGAACTTAGTGACCCT+TGG | 0.490528 | 3.3:-95872626 | None:intergenic |
| GGTCAAGAGTTTATTCGTGT+TGG | 0.494710 | 3.3:+95872503 | MS.gene063084:CDS |
| TAGGAAATCTCGAACTGAAA+GGG | 0.513564 | 3.3:-95871993 | None:intergenic |
| AAGGGATTGAGAAACGAAGC+TGG | 0.517879 | 3.3:-95871975 | None:intergenic |
| GTTTGGGGTAGTTGCTCTGA+GGG | 0.518854 | 3.3:-95872692 | None:intergenic |
| CGATAGTTTCCAACATTGAC+GGG | 0.519253 | 3.3:-95872386 | None:intergenic |
| GAGAGAGGAACCTCCAACCA+AGG | 0.524167 | 3.3:+95872562 | MS.gene063084:CDS |
| ATTCGTGAAGAAGATATCAT+TGG | 0.528304 | 3.3:+95872446 | MS.gene063084:CDS |
| ATAGGAAATCTCGAACTGAA+AGG | 0.536457 | 3.3:-95871994 | None:intergenic |
| CTTGTTGGTCCCGTCAATGT+TGG | 0.539469 | 3.3:+95872377 | MS.gene063084:CDS |
| CGAATCTTGGATGGGTCTGG+TGG | 0.541608 | 3.3:-95872428 | None:intergenic |
| TGTTTGGCCGCTCTCAAAGA+TGG | 0.557561 | 3.3:+95872020 | MS.gene063084:CDS |
| CTCTTGACCCAAATAAGAGC+AGG | 0.559683 | 3.3:-95872489 | None:intergenic |
| AAATTGTCACAGATTTGGAA+TGG | 0.563329 | 3.3:+95872294 | MS.gene063084:intron |
| CTTCTTCACGAATCTTGGAT+GGG | 0.564124 | 3.3:-95872436 | None:intergenic |
| GGATCTTGTTCTCCAGTTTG+GGG | 0.567661 | 3.3:-95872707 | None:intergenic |
| GCGATAGTTTCCAACATTGA+CGG | 0.581777 | 3.3:-95872387 | None:intergenic |
| AGTTTGGGGTAGTTGCTCTG+AGG | 0.582073 | 3.3:-95872693 | None:intergenic |
| GTATCGGGTGCAGCAGCAAG+TGG | 0.584533 | 3.3:-95872728 | None:intergenic |
| TGACGACGAGCAGCTGAGAG+AGG | 0.624627 | 3.3:+95872547 | MS.gene063084:CDS |
| CAACATTGACGGGACCAACA+AGG | 0.634986 | 3.3:-95872376 | None:intergenic |
| ACATCTTATCTGATAAACCA+AGG | 0.636524 | 3.3:+95872609 | MS.gene063084:CDS |
| GATTTATGTTGGATCTGCTG+AGG | 0.636648 | 3.3:+95872322 | MS.gene063084:CDS |
| GAAACGAAGCTGGATTGTCA+AGG | 0.636849 | 3.3:-95871965 | None:intergenic |
| AAGAAGAAAAGCGATGAGCG+CGG | 0.647668 | 3.3:+95871920 | None:intergenic |
| CATCTTATCTGATAAACCAA+GGG | 0.651929 | 3.3:+95872610 | MS.gene063084:CDS |
| AGAGCAGGTGAGTAGAAGCA+CGG | 0.669211 | 3.3:-95872474 | None:intergenic |
| GGTTAACATTACGAACGTGA+CGG | 0.673152 | 3.3:+95871941 | MS.gene063084:CDS |
| TCTCAGGGTGGAAATTGATG+GGG | 0.674973 | 3.3:-95872643 | None:intergenic |
| ATACATACCATCTTTGAGAG+CGG | 0.695677 | 3.3:-95872027 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATATAGTTAAATTGCAT+TGG | - | chr3.3:95872264-95872283 | None:intergenic | 15.0% |
| !! | TAATATAGTTAAATTGCATT+GGG | - | chr3.3:95872263-95872282 | None:intergenic | 15.0% |
| !!! | TTTACTCTACTTTTATTTTA+GGG | + | chr3.3:95872058-95872077 | MS.gene063084:intron | 15.0% |
| !! | AAACAAAATTAGGTCAAATT+TGG | - | chr3.3:95872165-95872184 | None:intergenic | 20.0% |
| !! | AACAAAATTAGGTCAAATTT+GGG | - | chr3.3:95872164-95872183 | None:intergenic | 20.0% |
| !! | AATATAGTTAAATTGCATTG+GGG | - | chr3.3:95872262-95872281 | None:intergenic | 20.0% |
| !! | ACTAAGTAACAAACAAAATT+AGG | - | chr3.3:95872175-95872194 | None:intergenic | 20.0% |
| !!! | CTTTACTCTACTTTTATTTT+AGG | + | chr3.3:95872057-95872076 | MS.gene063084:intron | 20.0% |
| !!! | TCTACTTTCTTGTTTTTTTA+GGG | + | chr3.3:95872213-95872232 | MS.gene063084:intron | 20.0% |
| ! | ATATAGTTAAATTGCATTGG+GGG | - | chr3.3:95872261-95872280 | None:intergenic | 25.0% |
| ! | CTAAAAAAACAAGAAAGTAG+AGG | - | chr3.3:95872214-95872233 | None:intergenic | 25.0% |
| !! | GTTTGTTACTTAGTTAGTTA+GGG | + | chr3.3:95872181-95872200 | MS.gene063084:intron | 25.0% |
| !! | TGTTTGTTACTTAGTTAGTT+AGG | + | chr3.3:95872180-95872199 | MS.gene063084:intron | 25.0% |
| !!! | CTCTACTTTCTTGTTTTTTT+AGG | + | chr3.3:95872212-95872231 | MS.gene063084:intron | 25.0% |
| !!! | TTCTTGTTTTTTTAGGGTTT+AGG | + | chr3.3:95872219-95872238 | MS.gene063084:intron | 25.0% |
| AAAACAAGAAAGTAGAGGAT+TGG | - | chr3.3:95872209-95872228 | None:intergenic | 30.0% | |
| AAATTGTCACAGATTTGGAA+TGG | + | chr3.3:95872294-95872313 | MS.gene063084:intron | 30.0% | |
| ACATCTTATCTGATAAACCA+AGG | + | chr3.3:95872609-95872628 | MS.gene063084:CDS | 30.0% | |
| ATTCGTGAAGAAGATATCAT+TGG | + | chr3.3:95872446-95872465 | MS.gene063084:CDS | 30.0% | |
| CATCTTATCTGATAAACCAA+GGG | + | chr3.3:95872610-95872629 | MS.gene063084:CDS | 30.0% | |
| GTTGAAAATTGTCACAGATT+TGG | + | chr3.3:95872289-95872308 | MS.gene063084:intron | 30.0% | |
| TATAGTTAAATTGCATTGGG+GGG | - | chr3.3:95872260-95872279 | None:intergenic | 30.0% | |
| ! | CTTCATTTTCATTGTTCTCA+GGG | - | chr3.3:95872661-95872680 | None:intergenic | 30.0% |
| ! | TCTTCATTTTCATTGTTCTC+AGG | - | chr3.3:95872662-95872681 | None:intergenic | 30.0% |
| ! | TTTTAACAGATAGGGTTCAA+AGG | + | chr3.3:95872585-95872604 | MS.gene063084:CDS | 30.0% |
| !! | AACCAAGGTTTTAACAGATA+GGG | + | chr3.3:95872577-95872596 | MS.gene063084:CDS | 30.0% |
| !!! | AAACTATCGCTTTGTTTTAC+AGG | + | chr3.3:95872400-95872419 | MS.gene063084:CDS | 30.0% |
| AACCCTATCTGTTAAAACCT+TGG | - | chr3.3:95872582-95872601 | None:intergenic | 35.0% | |
| ATAGGAAATCTCGAACTGAA+AGG | - | chr3.3:95871997-95872016 | None:intergenic | 35.0% | |
| CTATCTGTTAAAACCTTGGT+TGG | - | chr3.3:95872578-95872597 | None:intergenic | 35.0% | |
| GATATCTTCTTCACGAATCT+TGG | - | chr3.3:95872444-95872463 | None:intergenic | 35.0% | |
| TAGGAAATCTCGAACTGAAA+GGG | - | chr3.3:95871996-95872015 | None:intergenic | 35.0% | |
| TGAGACTTATGACCAGTTAT+TGG | + | chr3.3:95872346-95872365 | MS.gene063084:CDS | 35.0% | |
| ! | ATACATACCATCTTTGAGAG+CGG | - | chr3.3:95872030-95872049 | None:intergenic | 35.0% |
| ! | ATTTTCGTTTTCACTCTGCT+AGG | + | chr3.3:95872098-95872117 | MS.gene063084:intron | 35.0% |
| ! | GAATGGAAGCTGATTTATGT+TGG | + | chr3.3:95872311-95872330 | MS.gene063084:CDS | 35.0% |
| !! | CAACCAAGGTTTTAACAGAT+AGG | + | chr3.3:95872576-95872595 | MS.gene063084:CDS | 35.0% |
| CGAGATTTCCTATGAGTGTT+TGG | + | chr3.3:95872004-95872023 | MS.gene063084:CDS | 40.0% | |
| CGATAGTTTCCAACATTGAC+GGG | - | chr3.3:95872389-95872408 | None:intergenic | 40.0% | |
| CTTCTTCACGAATCTTGGAT+GGG | - | chr3.3:95872439-95872458 | None:intergenic | 40.0% | |
| GATTTATGTTGGATCTGCTG+AGG | + | chr3.3:95872322-95872341 | MS.gene063084:CDS | 40.0% | |
| GCGATAGTTTCCAACATTGA+CGG | - | chr3.3:95872390-95872409 | None:intergenic | 40.0% | |
| GGTCAAGAGTTTATTCGTGT+TGG | + | chr3.3:95872503-95872522 | MS.gene063084:CDS | 40.0% | |
| GGTTAACATTACGAACGTGA+CGG | + | chr3.3:95871941-95871960 | MS.gene063084:CDS | 40.0% | |
| TCTACTCACCTGCTCTTATT+TGG | + | chr3.3:95872481-95872500 | MS.gene063084:CDS | 40.0% | |
| TCTGTTAAAACCTTGGTTGG+AGG | - | chr3.3:95872575-95872594 | None:intergenic | 40.0% | |
| TCTTCTTCACGAATCTTGGA+TGG | - | chr3.3:95872440-95872459 | None:intergenic | 40.0% | |
| TGGATCTTGTTCTCCAGTTT+GGG | - | chr3.3:95872711-95872730 | None:intergenic | 40.0% | |
| TTCTCAGGGTGGAAATTGAT+GGG | - | chr3.3:95872647-95872666 | None:intergenic | 40.0% | |
| ! | CATTTTCATTGTTCTCAGGG+TGG | - | chr3.3:95872658-95872677 | None:intergenic | 40.0% |
| ! | CTACTCACCTGCTCTTATTT+GGG | + | chr3.3:95872482-95872501 | MS.gene063084:CDS | 40.0% |
| ! | GTTTTCACTCTGCTAGGTTA+GGG | + | chr3.3:95872104-95872123 | MS.gene063084:intron | 40.0% |
| !! | TTATTGGAGAGTGTCCTTGT+TGG | + | chr3.3:95872362-95872381 | MS.gene063084:CDS | 40.0% |
| AAGGGATTGAGAAACGAAGC+TGG | - | chr3.3:95871978-95871997 | None:intergenic | 45.0% | |
| ACTCTGCTAGGTTAGGGTTT+AGG | + | chr3.3:95872110-95872129 | MS.gene063084:intron | 45.0% | |
| AGTTAAATTGCATTGGGGGG+AGG | - | chr3.3:95872257-95872276 | None:intergenic | 45.0% | |
| ATACCATTCCCCCAAATGAC+GGG | + | chr3.3:95872747-95872766 | MS.gene063084:CDS | 45.0% | |
| CAAGGACACTCTCCAATAAC+TGG | - | chr3.3:95872361-95872380 | None:intergenic | 45.0% | |
| CTCTGCTAGGTTAGGGTTTA+GGG | + | chr3.3:95872111-95872130 | MS.gene063084:intron | 45.0% | |
| CTCTTGACCCAAATAAGAGC+AGG | - | chr3.3:95872492-95872511 | None:intergenic | 45.0% | |
| GAAACGAAGCTGGATTGTCA+AGG | - | chr3.3:95871968-95871987 | None:intergenic | 45.0% | |
| GATACCATTCCCCCAAATGA+CGG | + | chr3.3:95872746-95872765 | MS.gene063084:CDS | 45.0% | |
| GGATCTTGTTCTCCAGTTTG+GGG | - | chr3.3:95872710-95872729 | None:intergenic | 45.0% | |
| GTCATTTGGGGGAATGGTAT+CGG | - | chr3.3:95872747-95872766 | None:intergenic | 45.0% | |
| GTGGATCTTGTTCTCCAGTT+TGG | - | chr3.3:95872712-95872731 | None:intergenic | 45.0% | |
| GTTAAATTGCATTGGGGGGA+GGG | - | chr3.3:95872256-95872275 | None:intergenic | 45.0% | |
| GTTCTCAGGGTGGAAATTGA+TGG | - | chr3.3:95872648-95872667 | None:intergenic | 45.0% | |
| TCATTTGGGGGAATGGTATC+GGG | - | chr3.3:95872746-95872765 | None:intergenic | 45.0% | |
| TCTCAGGGTGGAAATTGATG+GGG | - | chr3.3:95872646-95872665 | None:intergenic | 45.0% | |
| TTAAATTGCATTGGGGGGAG+GGG | - | chr3.3:95872255-95872274 | None:intergenic | 45.0% | |
| TTAAGAACCCCCGTCATTTG+GGG | - | chr3.3:95872759-95872778 | None:intergenic | 45.0% | |
| ! | CGTTTTCACTCTGCTAGGTT+AGG | + | chr3.3:95872103-95872122 | MS.gene063084:intron | 45.0% |
| AGAGCAGGTGAGTAGAAGCA+CGG | - | chr3.3:95872477-95872496 | None:intergenic | 50.0% | |
| ATGGGGAACTTAGTGACCCT+TGG | - | chr3.3:95872629-95872648 | None:intergenic | 50.0% | |
| CAACATTGACGGGACCAACA+AGG | - | chr3.3:95872379-95872398 | None:intergenic | 50.0% | |
| CTTGTTGGTCCCGTCAATGT+TGG | + | chr3.3:95872377-95872396 | MS.gene063084:CDS | 50.0% | |
| TAAGAACCCCCGTCATTTGG+GGG | - | chr3.3:95872758-95872777 | None:intergenic | 50.0% | |
| TACCATTCCCCCAAATGACG+GGG | + | chr3.3:95872748-95872767 | MS.gene063084:CDS | 50.0% | |
| TCACGAATCTTGGATGGGTC+TGG | - | chr3.3:95872434-95872453 | None:intergenic | 50.0% | |
| TCAGAGCAACTACCCCAAAC+TGG | + | chr3.3:95872695-95872714 | MS.gene063084:CDS | 50.0% | |
| TGTTTGGCCGCTCTCAAAGA+TGG | + | chr3.3:95872020-95872039 | MS.gene063084:CDS | 50.0% | |
| ! | AGTTTGGGGTAGTTGCTCTG+AGG | - | chr3.3:95872696-95872715 | None:intergenic | 50.0% |
| ! | GTTTGGGGTAGTTGCTCTGA+GGG | - | chr3.3:95872695-95872714 | None:intergenic | 50.0% |
| ACCATTCCCCCAAATGACGG+GGG | + | chr3.3:95872749-95872768 | MS.gene063084:CDS | 55.0% | |
| CGAATCTTGGATGGGTCTGG+TGG | - | chr3.3:95872431-95872450 | None:intergenic | 55.0% | |
| GAGAGAGGAACCTCCAACCA+AGG | + | chr3.3:95872562-95872581 | MS.gene063084:CDS | 55.0% | |
| GAGAGCGGCCAAACACTCAT+AGG | - | chr3.3:95872015-95872034 | None:intergenic | 55.0% | |
| ACCCCCGTCATTTGGGGGAA+TGG | - | chr3.3:95872753-95872772 | None:intergenic | 60.0% | |
| GTATCGGGTGCAGCAGCAAG+TGG | - | chr3.3:95872731-95872750 | None:intergenic | 60.0% | |
| TGACGACGAGCAGCTGAGAG+AGG | + | chr3.3:95872547-95872566 | MS.gene063084:CDS | 60.0% | |
| !! | GGGGTAGTTGCTCTGAGGGA+GGG | - | chr3.3:95872691-95872710 | None:intergenic | 60.0% |
| !! | GGGTAGTTGCTCTGAGGGAG+GGG | - | chr3.3:95872690-95872709 | None:intergenic | 60.0% |
| !! | GGTAGTTGCTCTGAGGGAGG+GGG | - | chr3.3:95872689-95872708 | None:intergenic | 60.0% |
| !! | TGGGGTAGTTGCTCTGAGGG+AGG | - | chr3.3:95872692-95872711 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 95871933 | 95872778 | 95871933 | ID=MS.gene063084 |
| chr3.3 | mRNA | 95871933 | 95872778 | 95871933 | ID=MS.gene063084.t1;Parent=MS.gene063084 |
| chr3.3 | exon | 95871933 | 95872041 | 95871933 | ID=MS.gene063084.t1.exon1;Parent=MS.gene063084.t1 |
| chr3.3 | CDS | 95871933 | 95872041 | 95871933 | ID=cds.MS.gene063084.t1;Parent=MS.gene063084.t1 |
| chr3.3 | exon | 95872306 | 95872778 | 95872306 | ID=MS.gene063084.t1.exon2;Parent=MS.gene063084.t1 |
| chr3.3 | CDS | 95872306 | 95872778 | 95872306 | ID=cds.MS.gene063084.t1;Parent=MS.gene063084.t1 |
| Gene Sequence |
| Protein sequence |
