AlfalfaGEDB | MS.gene68733

M. sativa cultivar XinJiangDaYe / MS.gene68733

KEGG: K10753
GO: GO:0005575 GO:0005634 GO:0006325 GO:0006333 GO:0008150 GO:0009987 GO:0016043 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0071840 GO:0110165
PFAM: PF04729.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68733.t1	XP_020229252.1	91.7	193	14	2	1	193	1	191	2.10E-91	345.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68733.t1	Q9LS09	86.4	169	22	1	1	168	1	169	3.1e-80	299.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68733.t1	A0A1S3U1V4	95.6	180	7	1	1	180	1	179	4.3e-91	343.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene68733	MS.gene066035	PPI
MS.gene68733	MS.gene069975	PPI
MS.gene52970	MS.gene68733	PPI
MS.gene014301	MS.gene68733	PPI
MS.gene066034	MS.gene68733	PPI
MS.gene81646	MS.gene68733	PPI
MS.gene94307	MS.gene68733	PPI
MS.gene070538	MS.gene68733	PPI
MS.gene98552	MS.gene68733	PPI
MS.gene004833	MS.gene68733	PPI
MS.gene52972	MS.gene68733	PPI
MS.gene016712	MS.gene68733	PPI
MS.gene94315	MS.gene68733	PPI
MS.gene68733	MS.gene041630	PPI
MS.gene71415	MS.gene68733	PPI
MS.gene064876	MS.gene68733	PPI
MS.gene66836	MS.gene68733	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68733.t1	MTR_3g118150	96.373	193	7	0	1	193	1	193	5.38e-135	375

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68733.t1	AT5G38110	86.391	169	22	1	1	168	1	169	1.13e-104	300
MS.gene68733.t1	AT1G66740	80.899	178	31	1	1	178	1	175	2.33e-103	296

Find 50 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTACTCACATGCTCTTATT+TGG	0.139177	3.2:+93137173	MS.gene68733:CDS
TGGATCTTGTTCTCCAGTTT+GGG	0.231870	3.2:-93137400	None:intergenic
GTGGATCTTGTTCTCCAGTT+TGG	0.266545	3.2:-93137401	None:intergenic
CTACTCACATGCTCTTATTT+GGG	0.286175	3.2:+93137174	MS.gene68733:CDS
GATATCTTCTTCACGAATCT+TGG	0.287365	3.2:-93137133	None:intergenic
TGAGACTTATGACCAGTTAT+TGG	0.289525	3.2:+93137038	MS.gene68733:CDS
CGAGATTTCCTATGAGTGTT+TGG	0.353611	3.2:+93136680	MS.gene68733:CDS
GGGTAGTTGCTCTGAGGGAG+GGG	0.371206	3.2:-93137379	None:intergenic
TGGGGTAGTTGCTCTGAGGG+AGG	0.378053	3.2:-93137381	None:intergenic
GGGGTAGTTGCTCTGAGGGA+GGG	0.392562	3.2:-93137380	None:intergenic
GTTCTCAGGGTGGAAATTGA+TGG	0.403676	3.2:-93137337	None:intergenic
GGTAGTTGCTCTGAGGGAGG+GGG	0.407080	3.2:-93137378	None:intergenic
CAAGGACACTCTCCAATAAC+TGG	0.417188	3.2:-93137050	None:intergenic
TCACGAATCTTGGATGGGTC+TGG	0.425679	3.2:-93137123	None:intergenic
TCAGAGCAACTACCCCAAAC+TGG	0.436456	3.2:+93137387	MS.gene68733:CDS
GAGAGCGGCCAAACACTCAT+AGG	0.445137	3.2:-93136688	None:intergenic
CTATCTGTTAAAACCTTGGT+TGG	0.449270	3.2:-93137267	None:intergenic
AACCCTATCTGTTAAAACCT+TGG	0.452925	3.2:-93137271	None:intergenic
TTATTGGAGAGTGTCCTTGT+TGG	0.475012	3.2:+93137054	MS.gene68733:CDS
TTCTCAGGGTGGAAATTGAT+GGG	0.479943	3.2:-93137336	None:intergenic
TCTGTTAAAACCTTGGTTGG+AGG	0.483580	3.2:-93137264	None:intergenic
GAATGGAAGCTGATTTATGT+TGG	0.485287	3.2:+93137003	MS.gene68733:CDS
TCTTCTTCACGAATCTTGGA+TGG	0.488809	3.2:-93137129	None:intergenic
ATGGGGAACTTAGTGACCCT+TGG	0.490528	3.2:-93137318	None:intergenic
GGTCAAGAGTTTATTCGTGT+TGG	0.494710	3.2:+93137195	MS.gene68733:CDS
CTTGTTGGTCCTGTCAATGT+TGG	0.502550	3.2:+93137069	MS.gene68733:CDS
CGATAGTTTCCAACATTGAC+AGG	0.504203	3.2:-93137078	None:intergenic
TAGGAAATCTCGAACTGAAA+GGG	0.513564	3.2:-93136669	None:intergenic
AAGGGATTGAGAAACGAAGC+TGG	0.517879	3.2:-93136651	None:intergenic
GTTTGGGGTAGTTGCTCTGA+GGG	0.518854	3.2:-93137384	None:intergenic
GAGAGAGGAACCTCCAACCA+AGG	0.524167	3.2:+93137254	MS.gene68733:CDS
ATTCGTGAAGAAGATATCAT+TGG	0.528304	3.2:+93137138	MS.gene68733:CDS
ATAGGAAATCTCGAACTGAA+AGG	0.536457	3.2:-93136670	None:intergenic
CGAATCTTGGATGGGTCTGG+TGG	0.541608	3.2:-93137120	None:intergenic
TAATTGTCACAGATTTGGAA+TGG	0.548767	3.2:+93136986	MS.gene68733:intron
TGTTTGGCCGCTCTCAAAGA+TGG	0.557561	3.2:+93136696	MS.gene68733:CDS
CTTCTTCACGAATCTTGGAT+GGG	0.564124	3.2:-93137128	None:intergenic
GGATCTTGTTCTCCAGTTTG+GGG	0.567661	3.2:-93137399	None:intergenic
AGTTTGGGGTAGTTGCTCTG+AGG	0.582073	3.2:-93137385	None:intergenic
GTATCGGGTGCAGCAGCAAG+TGG	0.584533	3.2:-93137420	None:intergenic
CAACATTGACAGGACCAACA+AGG	0.622504	3.2:-93137068	None:intergenic
TGACGACGAGCAGCTGAGAG+AGG	0.624627	3.2:+93137239	MS.gene68733:CDS
ACATCTTATCTGATAAACCA+AGG	0.636524	3.2:+93137301	MS.gene68733:CDS
GATTTATGTTGGATCTGCTG+AGG	0.636648	3.2:+93137014	MS.gene68733:CDS
GAAACGAAGCTGGATTGTCA+AGG	0.636849	3.2:-93136641	None:intergenic
AAGAAGAAAAGCGATGAGCG+CGG	0.647668	3.2:+93136596	None:intergenic
CATCTTATCTGATAAACCAA+GGG	0.651929	3.2:+93137302	MS.gene68733:CDS
GGTTAACATTACGAACGTGA+CGG	0.673152	3.2:+93136617	MS.gene68733:CDS
TCTCAGGGTGGAAATTGATG+GGG	0.674973	3.2:-93137335	None:intergenic
ATACATACCATCTTTGAGAG+CGG	0.695677	3.2:-93136703	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTACTCTACTTTTATTTTA+GGG	+	chr3.2:93136734-93136753	MS.gene68733:intron	15.0%
!!	AAACAAAATTAGGTCAAATT+TGG	-	chr3.2:93136841-93136860	None:intergenic	20.0%
!!	AACAAAATTAGGTCAAATTT+GGG	-	chr3.2:93136840-93136859	None:intergenic	20.0%
!!	ACTAAGTAACAAACAAAATT+AGG	-	chr3.2:93136851-93136870	None:intergenic	20.0%
!!	TTCAACTTAATAATTGCATT+GGG	-	chr3.2:93136939-93136958	None:intergenic	20.0%
!!	TTTCAACTTAATAATTGCAT+TGG	-	chr3.2:93136940-93136959	None:intergenic	20.0%
!!!	CAATTATTAAGTTGAAAATG+TGG	+	chr3.2:93136943-93136962	MS.gene68733:intron	20.0%
!!!	CTTTACTCTACTTTTATTTT+AGG	+	chr3.2:93136733-93136752	MS.gene68733:intron	20.0%
!!!	TCTACTTTCTTGTTTTTTTA+GGG	+	chr3.2:93136889-93136908	MS.gene68733:intron	20.0%
!	CTAAAAAAACAAGAAAGTAG+AGG	-	chr3.2:93136890-93136909	None:intergenic	25.0%
!	TCAACTTAATAATTGCATTG+GGG	-	chr3.2:93136938-93136957	None:intergenic	25.0%
!!	GTTTGTTACTTAGTTAGTTA+GGG	+	chr3.2:93136857-93136876	MS.gene68733:intron	25.0%
!!	TGTTTGTTACTTAGTTAGTT+AGG	+	chr3.2:93136856-93136875	MS.gene68733:intron	25.0%
!!!	CTCTACTTTCTTGTTTTTTT+AGG	+	chr3.2:93136888-93136907	MS.gene68733:intron	25.0%
!!!	TTCTTGTTTTTTTAGGGTTT+AGG	+	chr3.2:93136895-93136914	MS.gene68733:intron	25.0%
	AAAACAAGAAAGTAGAGGAT+TGG	-	chr3.2:93136885-93136904	None:intergenic	30.0%
	AACTTAATAATTGCATTGGG+GGG	-	chr3.2:93136936-93136955	None:intergenic	30.0%
	ACATCTTATCTGATAAACCA+AGG	+	chr3.2:93137301-93137320	MS.gene68733:CDS	30.0%
	ATGGTTAATTGTCACAGATT+TGG	+	chr3.2:93136981-93137000	MS.gene68733:intron	30.0%
	ATTCGTGAAGAAGATATCAT+TGG	+	chr3.2:93137138-93137157	MS.gene68733:CDS	30.0%
	CAACTTAATAATTGCATTGG+GGG	-	chr3.2:93136937-93136956	None:intergenic	30.0%
	CATCTTATCTGATAAACCAA+GGG	+	chr3.2:93137302-93137321	MS.gene68733:CDS	30.0%
	TAATTGTCACAGATTTGGAA+TGG	+	chr3.2:93136986-93137005	MS.gene68733:intron	30.0%
!	CTTCATTTTCATTGTTCTCA+GGG	-	chr3.2:93137353-93137372	None:intergenic	30.0%
!	TCTTCATTTTCATTGTTCTC+AGG	-	chr3.2:93137354-93137373	None:intergenic	30.0%
!	TTTTAACAGATAGGGTTCAA+AGG	+	chr3.2:93137277-93137296	MS.gene68733:CDS	30.0%
!!	AACCAAGGTTTTAACAGATA+GGG	+	chr3.2:93137269-93137288	MS.gene68733:CDS	30.0%
!!!	AAACTATCGCTTTGTTTTAC+AGG	+	chr3.2:93137092-93137111	MS.gene68733:CDS	30.0%
	AACCCTATCTGTTAAAACCT+TGG	-	chr3.2:93137274-93137293	None:intergenic	35.0%
	ATAGGAAATCTCGAACTGAA+AGG	-	chr3.2:93136673-93136692	None:intergenic	35.0%
	CTATCTGTTAAAACCTTGGT+TGG	-	chr3.2:93137270-93137289	None:intergenic	35.0%
	GATATCTTCTTCACGAATCT+TGG	-	chr3.2:93137136-93137155	None:intergenic	35.0%
	TAGGAAATCTCGAACTGAAA+GGG	-	chr3.2:93136672-93136691	None:intergenic	35.0%
	TCTACTCACATGCTCTTATT+TGG	+	chr3.2:93137173-93137192	MS.gene68733:CDS	35.0%
	TGAGACTTATGACCAGTTAT+TGG	+	chr3.2:93137038-93137057	MS.gene68733:CDS	35.0%
!	ATACATACCATCTTTGAGAG+CGG	-	chr3.2:93136706-93136725	None:intergenic	35.0%
!	ATTTTCGTTTTCACTCTGCT+AGG	+	chr3.2:93136774-93136793	MS.gene68733:intron	35.0%
!	CTACTCACATGCTCTTATTT+GGG	+	chr3.2:93137174-93137193	MS.gene68733:CDS	35.0%
!	GAATGGAAGCTGATTTATGT+TGG	+	chr3.2:93137003-93137022	MS.gene68733:CDS	35.0%
!	GTGGACTATCAATTCATTCA+TGG	+	chr3.2:93136962-93136981	MS.gene68733:intron	35.0%
!!	CAACCAAGGTTTTAACAGAT+AGG	+	chr3.2:93137268-93137287	MS.gene68733:CDS	35.0%
	CGAGATTTCCTATGAGTGTT+TGG	+	chr3.2:93136680-93136699	MS.gene68733:CDS	40.0%
	CGATAGTTTCCAACATTGAC+AGG	-	chr3.2:93137081-93137100	None:intergenic	40.0%
	CTTCTTCACGAATCTTGGAT+GGG	-	chr3.2:93137131-93137150	None:intergenic	40.0%
	GATTTATGTTGGATCTGCTG+AGG	+	chr3.2:93137014-93137033	MS.gene68733:CDS	40.0%
	GGTCAAGAGTTTATTCGTGT+TGG	+	chr3.2:93137195-93137214	MS.gene68733:CDS	40.0%
	GGTTAACATTACGAACGTGA+CGG	+	chr3.2:93136617-93136636	MS.gene68733:CDS	40.0%
	TAATAATTGCATTGGGGGGA+GGG	-	chr3.2:93136932-93136951	None:intergenic	40.0%
	TCTGTTAAAACCTTGGTTGG+AGG	-	chr3.2:93137267-93137286	None:intergenic	40.0%
	TCTTCTTCACGAATCTTGGA+TGG	-	chr3.2:93137132-93137151	None:intergenic	40.0%
	TGGATCTTGTTCTCCAGTTT+GGG	-	chr3.2:93137403-93137422	None:intergenic	40.0%
	TTAATAATTGCATTGGGGGG+AGG	-	chr3.2:93136933-93136952	None:intergenic	40.0%
	TTCTCAGGGTGGAAATTGAT+GGG	-	chr3.2:93137339-93137358	None:intergenic	40.0%
!	CATTTTCATTGTTCTCAGGG+TGG	-	chr3.2:93137350-93137369	None:intergenic	40.0%
!	GTTTTCACTCTGCTAGGTTA+GGG	+	chr3.2:93136780-93136799	MS.gene68733:intron	40.0%
!!	TTATTGGAGAGTGTCCTTGT+TGG	+	chr3.2:93137054-93137073	MS.gene68733:CDS	40.0%
	AAGGGATTGAGAAACGAAGC+TGG	-	chr3.2:93136654-93136673	None:intergenic	45.0%
	AATAATTGCATTGGGGGGAG+GGG	-	chr3.2:93136931-93136950	None:intergenic	45.0%
	ACTCTGCTAGGTTAGGGTTT+AGG	+	chr3.2:93136786-93136805	MS.gene68733:intron	45.0%
	ATACCATTCCCCCAAATGAG+GGG	+	chr3.2:93137439-93137458	MS.gene68733:CDS	45.0%
	CAACATTGACAGGACCAACA+AGG	-	chr3.2:93137071-93137090	None:intergenic	45.0%
	CAAGGACACTCTCCAATAAC+TGG	-	chr3.2:93137053-93137072	None:intergenic	45.0%
	CTCATTTGGGGGAATGGTAT+CGG	-	chr3.2:93137439-93137458	None:intergenic	45.0%
	CTCTGCTAGGTTAGGGTTTA+GGG	+	chr3.2:93136787-93136806	MS.gene68733:intron	45.0%
	CTTGTTGGTCCTGTCAATGT+TGG	+	chr3.2:93137069-93137088	MS.gene68733:CDS	45.0%
	GAAACGAAGCTGGATTGTCA+AGG	-	chr3.2:93136644-93136663	None:intergenic	45.0%
	GATACCATTCCCCCAAATGA+GGG	+	chr3.2:93137438-93137457	MS.gene68733:CDS	45.0%
	GGATCTTGTTCTCCAGTTTG+GGG	-	chr3.2:93137402-93137421	None:intergenic	45.0%
	GTGGATCTTGTTCTCCAGTT+TGG	-	chr3.2:93137404-93137423	None:intergenic	45.0%
	GTTCTCAGGGTGGAAATTGA+TGG	-	chr3.2:93137340-93137359	None:intergenic	45.0%
	TCATTTGGGGGAATGGTATC+GGG	-	chr3.2:93137438-93137457	None:intergenic	45.0%
	TCTCAGGGTGGAAATTGATG+GGG	-	chr3.2:93137338-93137357	None:intergenic	45.0%
	TTAAGAACCCCCCTCATTTG+GGG	-	chr3.2:93137451-93137470	None:intergenic	45.0%
!	CGTTTTCACTCTGCTAGGTT+AGG	+	chr3.2:93136779-93136798	MS.gene68733:intron	45.0%
	ATGGGGAACTTAGTGACCCT+TGG	-	chr3.2:93137321-93137340	None:intergenic	50.0%
	CGATACCATTCCCCCAAATG+AGG	+	chr3.2:93137437-93137456	MS.gene68733:CDS	50.0%
	TAAGAACCCCCCTCATTTGG+GGG	-	chr3.2:93137450-93137469	None:intergenic	50.0%
	TACCATTCCCCCAAATGAGG+GGG	+	chr3.2:93137440-93137459	MS.gene68733:CDS	50.0%
	TCACGAATCTTGGATGGGTC+TGG	-	chr3.2:93137126-93137145	None:intergenic	50.0%
	TCAGAGCAACTACCCCAAAC+TGG	+	chr3.2:93137387-93137406	MS.gene68733:CDS	50.0%
	TGTTTGGCCGCTCTCAAAGA+TGG	+	chr3.2:93136696-93136715	MS.gene68733:CDS	50.0%
!	AGTTTGGGGTAGTTGCTCTG+AGG	-	chr3.2:93137388-93137407	None:intergenic	50.0%
!	GTTTGGGGTAGTTGCTCTGA+GGG	-	chr3.2:93137387-93137406	None:intergenic	50.0%
	ACCATTCCCCCAAATGAGGG+GGG	+	chr3.2:93137441-93137460	MS.gene68733:CDS	55.0%
	CGAATCTTGGATGGGTCTGG+TGG	-	chr3.2:93137123-93137142	None:intergenic	55.0%
	GAGAGAGGAACCTCCAACCA+AGG	+	chr3.2:93137254-93137273	MS.gene68733:CDS	55.0%
	GAGAGCGGCCAAACACTCAT+AGG	-	chr3.2:93136691-93136710	None:intergenic	55.0%
	ACCCCCCTCATTTGGGGGAA+TGG	-	chr3.2:93137445-93137464	None:intergenic	60.0%
	GTATCGGGTGCAGCAGCAAG+TGG	-	chr3.2:93137423-93137442	None:intergenic	60.0%
	TGACGACGAGCAGCTGAGAG+AGG	+	chr3.2:93137239-93137258	MS.gene68733:CDS	60.0%
!!	GGGGTAGTTGCTCTGAGGGA+GGG	-	chr3.2:93137383-93137402	None:intergenic	60.0%
!!	GGGTAGTTGCTCTGAGGGAG+GGG	-	chr3.2:93137382-93137401	None:intergenic	60.0%
!!	GGTAGTTGCTCTGAGGGAGG+GGG	-	chr3.2:93137381-93137400	None:intergenic	60.0%
!!	TGGGGTAGTTGCTCTGAGGG+AGG	-	chr3.2:93137384-93137403	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	93136609	93137470	93136609	ID=MS.gene68733
chr3.2	mRNA	93136609	93137470	93136609	ID=MS.gene68733.t1;Parent=MS.gene68733
chr3.2	exon	93136609	93136717	93136609	ID=MS.gene68733.t1.exon1;Parent=MS.gene68733.t1
chr3.2	CDS	93136609	93136717	93136609	ID=cds.MS.gene68733.t1;Parent=MS.gene68733.t1
chr3.2	exon	93136998	93137470	93136998	ID=MS.gene68733.t1.exon2;Parent=MS.gene68733.t1
chr3.2	CDS	93136998	93137470	93136998	ID=cds.MS.gene68733.t1;Parent=MS.gene68733.t1

Gene Sequence

>MS.gene68733

ATGAGCGCGGTTAACATTACGAACGTGACGGTCCTTGACAATCCAGCTTCGTTTCTCAATCCCTTTCAGTTCGAGATTTCCTATGAGTGTTTGGCCGCTCTCAAAGATGGTATGTATCGTTTCTCTTTACTCTACTTTTATTTTAGGGTTTAGATCTTCAATTCAATTTTCGTTTTCACTCTGCTAGGTTAGGGTTTAGGGTTTTTCTTTTCAGATCTTGTTTCTGTTCCCAAATTTGACCTAATTTTGTTTGTTACTTAGTTAGTTAGGGCACCAATCCTCTACTTTCTTGTTTTTTTAGGGTTTAGGCTGTTCTACTCCCCTCCCCCCAATGCAATTATTAAGTTGAAAATGTGGACTATCAATTCATTCATGGTTAATTGTCACAGATTTGGAATGGAAGCTGATTTATGTTGGATCTGCTGAGGATGAGACTTATGACCAGTTATTGGAGAGTGTCCTTGTTGGTCCTGTCAATGTTGGAAACTATCGCTTTGTTTTACAGGCAGATCCACCAGACCCATCCAAGATTCGTGAAGAAGATATCATTGGTGTTACAGTGCTTCTACTCACATGCTCTTATTTGGGTCAAGAGTTTATTCGTGTTGGCTACTATGTCAACAATGACTATGACGACGAGCAGCTGAGAGAGGAACCTCCAACCAAGGTTTTAACAGATAGGGTTCAAAGGAACATCTTATCTGATAAACCAAGGGTCACTAAGTTCCCCATCAATTTCCACCCTGAGAACAATGAAAATGAAGAACAACCCCCTCCCTCAGAGCAACTACCCCAAACTGGAGAACAAGATCCACTTGCTGCTGCACCCGATACCATTCCCCCAAATGAGGGGGGTTCTTAA

Protein sequence

>MS.gene68733.t1

MSAVNITNVTVLDNPASFLNPFQFEISYECLAALKDDLEWKLIYVGSAEDETYDQLLESVLVGPVNVGNYRFVLQADPPDPSKIREEDIIGVTVLLLTCSYLGQEFIRVGYYVNNDYDDEQLREEPPTKVLTDRVQRNILSDKPRVTKFPINFHPENNENEEQPPPSEQLPQTGEQDPLAAAPDTIPPNEGGS