Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041630.t1 | XP_003609712.1 | 97.7 | 132 | 3 | 0 | 14 | 145 | 2 | 133 | 7.50E-62 | 246.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041630.t1 | P55844 | 91.7 | 132 | 11 | 0 | 14 | 145 | 2 | 133 | 9.3e-61 | 234.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041630.t1 | B7FL54 | 97.7 | 132 | 3 | 0 | 14 | 145 | 2 | 133 | 5.4e-62 | 246.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77100 | MS.gene041630 | PPI |
| MS.gene68134 | MS.gene041630 | PPI |
| MS.gene063084 | MS.gene041630 | PPI |
| MS.gene52970 | MS.gene041630 | PPI |
| MS.gene066034 | MS.gene041630 | PPI |
| MS.gene014197 | MS.gene041630 | PPI |
| MS.gene68733 | MS.gene041630 | PPI |
| MS.gene52972 | MS.gene041630 | PPI |
| MS.gene066035 | MS.gene041630 | PPI |
| MS.gene066761 | MS.gene041630 | PPI |
| MS.gene71415 | MS.gene041630 | PPI |
| MS.gene004833 | MS.gene041630 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041630.t1 | MTR_4g120770 | 97.727 | 132 | 3 | 0 | 14 | 145 | 2 | 133 | 7.40e-89 | 254 |
| MS.gene041630.t1 | MTR_1g075720 | 93.939 | 132 | 8 | 0 | 14 | 145 | 2 | 133 | 6.03e-87 | 250 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041630.t1 | AT2G20450 | 83.206 | 131 | 22 | 0 | 15 | 145 | 3 | 133 | 1.10e-75 | 221 |
| MS.gene041630.t1 | AT4G27090 | 83.594 | 128 | 21 | 0 | 15 | 142 | 3 | 130 | 1.50e-74 | 218 |
Find 0 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAACAAAAGAAA+TGG | - | 32523:35267-35286 | None:intergenic | 10.0% |
| !! | ATCTTTATAATAAAAAAACA+CGG | - | 32523:35782-35801 | None:intergenic | 10.0% |
| !!! | AGTTTTTTAATGTTAAATTT+AGG | + | 32523:36421-36440 | MS.gene041630:intron | 10.0% |
| !! | ATTAAGTCATTTATTCAAAT+TGG | + | 32523:35756-35775 | MS.gene041630:intron | 15.0% |
| !! | TGTTTACTAACTAATTTAAA+GGG | - | 32523:36990-37009 | None:intergenic | 15.0% |
| !! | TTGTTTACTAACTAATTTAA+AGG | - | 32523:36991-37010 | None:intergenic | 15.0% |
| !!! | AAAAAATGAATTTTTTCATC+TGG | + | 32523:35850-35869 | MS.gene041630:intron | 15.0% |
| !!! | AAAAATGAATTTTTTCATCT+GGG | + | 32523:35851-35870 | MS.gene041630:intron | 15.0% |
| !!! | AAAGTTTGAATTTTTTTATC+TGG | + | 32523:35358-35377 | MS.gene041630:intron | 15.0% |
| !!! | ATTGATATCTTGTTATATTA+AGG | + | 32523:36541-36560 | MS.gene041630:intron | 15.0% |
| !!! | TATTTCTATGACAAAAAATA+AGG | - | 32523:35686-35705 | None:intergenic | 15.0% |
| !!! | TTAAAACAATCTTATTTCTT+AGG | + | 32523:36676-36695 | MS.gene041630:intron | 15.0% |
| !! | AAGAAACAAAACCTAAAAAT+TGG | - | 32523:35322-35341 | None:intergenic | 20.0% |
| !! | AATATTTGTAATCAAAATGC+AGG | - | 32523:36278-36297 | None:intergenic | 20.0% |
| !! | AATCTTTCTAATGAAAATAC+AGG | - | 32523:36775-36794 | None:intergenic | 20.0% |
| !! | AGAAACAAAACCTAAAAATT+GGG | - | 32523:35321-35340 | None:intergenic | 20.0% |
| !! | ATATTTGTAATCAAAATGCA+GGG | - | 32523:36277-36296 | None:intergenic | 20.0% |
| !! | CAGAAACAAAAAAAAAGATT+AGG | - | 32523:37530-37549 | None:intergenic | 20.0% |
| !! | TAAAATTACATACTCTAGTT+TGG | - | 32523:35239-35258 | None:intergenic | 20.0% |
| !! | TAAATAGAACAAATCAAAAC+TGG | - | 32523:35965-35984 | None:intergenic | 20.0% |
| !! | TTGCAAATATATGAATGTAA+TGG | + | 32523:36942-36961 | MS.gene041630:intron | 20.0% |
| !! | TTTCTGATGCTTTATAATTA+CGG | + | 32523:37565-37584 | MS.gene041630:intron | 20.0% |
| !!! | ATTAAGTTGTTTATCCATAT+AGG | + | 32523:36251-36270 | MS.gene041630:intron | 20.0% |
| !!! | GTATAAACTATTTCTATAAC+AGG | - | 32523:36189-36208 | None:intergenic | 20.0% |
| !!! | TTTTGTTTTGTTCTATTTCA+AGG | + | 32523:35471-35490 | MS.gene041630:intron | 20.0% |
| ! | AATGAACTTGACACTAATTT+AGG | - | 32523:36919-36938 | None:intergenic | 25.0% |
| ! | ACTTAACTTAATGCAAAGAA+TGG | - | 32523:35507-35526 | None:intergenic | 25.0% |
| ! | AGCAAATGAATGTTAATTGA+AGG | - | 32523:35111-35130 | None:intergenic | 25.0% |
| ! | AGTAATAAATAGTGCAGTAT+TGG | - | 32523:37093-37112 | None:intergenic | 25.0% |
| ! | ATCAAGAAATATTTGCTTGT+GGG | - | 32523:37132-37151 | None:intergenic | 25.0% |
| ! | ATTTAGGAACAACAAAAATG+TGG | - | 32523:36903-36922 | None:intergenic | 25.0% |
| ! | GCATTGTCAGAAAAAATTTA+TGG | + | 32523:35407-35426 | MS.gene041630:intron | 25.0% |
| ! | TACCAGAAAAAAAAAAACTG+TGG | + | 32523:35902-35921 | MS.gene041630:intron | 25.0% |
| ! | TATTTCTTAGGTTATACCTA+AGG | + | 32523:36688-36707 | MS.gene041630:intron | 25.0% |
| ! | TTTAAAGGGAATAAGAAACA+TGG | - | 32523:36976-36995 | None:intergenic | 25.0% |
| ! | TTTAGGAACAACAAAAATGT+GGG | - | 32523:36902-36921 | None:intergenic | 25.0% |
| ! | TTTATGGTAGAATTTGATTG+AGG | + | 32523:35423-35442 | MS.gene041630:intron | 25.0% |
| !! | TGTTCATGTTTTGCATTTAT+TGG | + | 32523:35994-36013 | MS.gene041630:intron | 25.0% |
| !! | TTGAACTAATTTGGTAATCT+AGG | + | 32523:36021-36040 | MS.gene041630:intron | 25.0% |
| !!! | AATCTAGGTATTGATGTATT+GGG | + | 32523:36036-36055 | MS.gene041630:intron | 25.0% |
| !!! | AGTTAAGTTTTGAACTGATT+TGG | + | 32523:35519-35538 | MS.gene041630:intron | 25.0% |
| !!! | ATTGGTGTTTTGAACTAATT+TGG | + | 32523:36012-36031 | MS.gene041630:intron | 25.0% |
| !!! | CAGTTTTTTTTTTTCTGGTA+AGG | - | 32523:35902-35921 | None:intergenic | 25.0% |
| !!! | TAATCTAGGTATTGATGTAT+TGG | + | 32523:36035-36054 | MS.gene041630:intron | 25.0% |
| !!! | TACCACAGTTTTTTTTTTTC+TGG | - | 32523:35907-35926 | None:intergenic | 25.0% |
| !!! | TGTTTTAGGTAGTGATATTA+AGG | + | 32523:35542-35561 | MS.gene041630:intron | 25.0% |
| !!! | TTGAGCAAATTTGTTAATCT+CGG | + | 32523:36517-36536 | MS.gene041630:intron | 25.0% |
| !!! | TTTTGATTTGTTCTACTTCA+AGG | + | 32523:36461-36480 | MS.gene041630:intron | 25.0% |
| AAACAGATGTTAAGAACAAG+TGG | + | 32523:37389-37408 | MS.gene041630:intron | 30.0% | |
| AAATACTAAGCGTTTATGCA+AGG | - | 32523:35576-35595 | None:intergenic | 30.0% | |
| AACAGATGTTAAGAACAAGT+GGG | + | 32523:37390-37409 | MS.gene041630:intron | 30.0% | |
| AGAGCCTCTTGAAATTTATA+GGG | - | 32523:37220-37239 | None:intergenic | 30.0% | |
| ATATGATGAGAAATTGTGCT+CGG | - | 32523:35064-35083 | None:intergenic | 30.0% | |
| ATCTCCTAGCATAAATACTT+GGG | + | 32523:36568-36587 | MS.gene041630:intron | 30.0% | |
| ATTCATTTGAGCACTATTCA+TGG | - | 32523:37041-37060 | None:intergenic | 30.0% | |
| GAACCTATCAAAATCATTGA+GGG | - | 32523:37464-37483 | None:intergenic | 30.0% | |
| GATCAAGAAATATTTGCTTG+TGG | - | 32523:37133-37152 | None:intergenic | 30.0% | |
| GTTAATTGAAGGAGAAAACT+TGG | - | 32523:35100-35119 | None:intergenic | 30.0% | |
| TCAAGAAATATTTGCTTGTG+GGG | - | 32523:37131-37150 | None:intergenic | 30.0% | |
| TGAACCTATCAAAATCATTG+AGG | - | 32523:37465-37484 | None:intergenic | 30.0% | |
| TTCATTTGAGCACTATTCAT+GGG | - | 32523:37040-37059 | None:intergenic | 30.0% | |
| TTGAAACTGCATAGCATAAT+TGG | + | 32523:36845-36864 | MS.gene041630:intron | 30.0% | |
| TTGTCTGCATCATTTATGTT+TGG | + | 32523:36301-36320 | MS.gene041630:intron | 30.0% | |
| TTTGTGTACTATATGTTGAC+AGG | + | 32523:37163-37182 | MS.gene041630:intron | 30.0% | |
| ! | AAACTACCAGATTCTTTTTC+TGG | - | 32523:36406-36425 | None:intergenic | 30.0% |
| ! | AACTACCAGATTCTTTTTCT+GGG | - | 32523:36405-36424 | None:intergenic | 30.0% |
| ! | ATGCAGTTTTTCACAAAAGT+TGG | - | 32523:37325-37344 | None:intergenic | 30.0% |
| ! | TGTCTGTGTTTTGCATTAAA+TGG | + | 32523:36490-36509 | MS.gene041630:intron | 30.0% |
| ! | TTGATGAGATCCTTTTTCTT+CGG | - | 32523:37278-37297 | None:intergenic | 30.0% |
| !! | GCTGTTTTCATAAGTTATCT+TGG | - | 32523:36127-36146 | None:intergenic | 30.0% |
| !! | TTTAGGTTTTCTATAGCTTC+TGG | + | 32523:36438-36457 | MS.gene041630:intron | 30.0% |
| !!! | GGTGTTGATAAATGTTTTCA+TGG | + | 32523:35872-35891 | MS.gene041630:intron | 30.0% |
| !!! | TTGAACTGATTTGGTGTTTT+AGG | + | 32523:35528-35547 | MS.gene041630:intron | 30.0% |
| !!! | TTTAGTTTTTGTATAGCTGC+TGG | + | 32523:35448-35467 | MS.gene041630:intron | 30.0% |
| AACTGATTGTGAAGAAGAGA+AGG | + | 32523:37434-37453 | MS.gene041630:CDS | 35.0% | |
| ACTGATTGTGAAGAAGAGAA+GGG | + | 32523:37435-37454 | MS.gene041630:CDS | 35.0% | |
| AGTATTTATGCTAGGAGATG+AGG | - | 32523:36567-36586 | None:intergenic | 35.0% | |
| ATAATTACGGCTTATGCAGA+GGG | + | 32523:37578-37597 | MS.gene041630:intron | 35.0% | |
| ATCAACTACGGAGAAAACTA+TGG | + | 32523:35183-35202 | MS.gene041630:CDS | 35.0% | |
| ATCTCAACGAATCTCTTGAA+AGG | - | 32523:35147-35166 | None:intergenic | 35.0% | |
| ATGAAAATACAGGCTGTAGT+TGG | - | 32523:36765-36784 | None:intergenic | 35.0% | |
| CAAGAAATATTTGCTTGTGG+GGG | - | 32523:37130-37149 | None:intergenic | 35.0% | |
| CATAAGCTCTTGTCAAGATA+GGG | - | 32523:36714-36733 | None:intergenic | 35.0% | |
| CATCTCCTAGCATAAATACT+TGG | + | 32523:36567-36586 | MS.gene041630:intron | 35.0% | |
| CCTAAAAATTGGGTACAAGA+TGG | - | 32523:35311-35330 | None:intergenic | 35.0% | |
| GAGAGCCTCTTGAAATTTAT+AGG | - | 32523:37221-37240 | None:intergenic | 35.0% | |
| GAGCCTCTTGAAATTTATAG+GGG | - | 32523:37219-37238 | None:intergenic | 35.0% | |
| GATTCCCAAGTATTTATGCT+AGG | - | 32523:36575-36594 | None:intergenic | 35.0% | |
| GCTGTCATCACAAGTTATAT+TGG | - | 32523:35632-35651 | None:intergenic | 35.0% | |
| TAAGAGAGTTCCGAAGAAAA+AGG | + | 32523:37265-37284 | MS.gene041630:CDS | 35.0% | |
| TAGTGCTCAAATGAATGTTG+TGG | + | 32523:37045-37064 | MS.gene041630:intron | 35.0% | |
| TATAATTACGGCTTATGCAG+AGG | + | 32523:37577-37596 | MS.gene041630:intron | 35.0% | |
| TATTGCCCAGAAAAAGAATC+TGG | + | 32523:36397-36416 | MS.gene041630:intron | 35.0% | |
| TCTTATTGATGCTCCAGATA+TGG | + | 32523:37187-37206 | MS.gene041630:CDS | 35.0% | |
| TGAATACCAAGTGTTTATGC+CGG | - | 32523:36072-36091 | None:intergenic | 35.0% | |
| TGATAGGTTCAAGATCATGT+TGG | + | 32523:37474-37493 | MS.gene041630:CDS | 35.0% | |
| TGCATAGCATAATTGGTGTA+GGG | + | 32523:36852-36871 | MS.gene041630:intron | 35.0% | |
| TGTATTGGGATATTATTCCC+CGG | + | 32523:36050-36069 | MS.gene041630:intron | 35.0% | |
| TTCAAGAGATTCGTTGAGAT+TGG | + | 32523:35147-35166 | MS.gene041630:CDS | 35.0% | |
| ! | CCATCTTGTACCCAATTTTT+AGG | + | 32523:35308-35327 | MS.gene041630:intron | 35.0% |
| !! | TTGAGTTGTCTGCATCTTTT+AGG | + | 32523:35801-35820 | MS.gene041630:intron | 35.0% |
| !!! | AGTTTTTTGTTCGCGACTTT+AGG | + | 32523:35926-35945 | MS.gene041630:intron | 35.0% |
| !!! | TTGTCTGCATCTTTTAGGTT+TGG | + | 32523:35806-35825 | MS.gene041630:intron | 35.0% |
| AAAGGATCTCATCAAAGCCA+TGG | + | 32523:37283-37302 | MS.gene041630:CDS | 40.0% | |
| AATACCAAGTGTTTATGCCG+GGG | - | 32523:36070-36089 | None:intergenic | 40.0% | |
| AGAGATTCGTTGAGATTGGA+AGG | + | 32523:35151-35170 | MS.gene041630:CDS | 40.0% | |
| AGCGTTTATGCAAGGAGATA+AGG | - | 32523:35568-35587 | None:intergenic | 40.0% | |
| ATAGTACACAAACTCAGCGA+TGG | - | 32523:37155-37174 | None:intergenic | 40.0% | |
| ATCAAAATGCAGGGCCTATA+TGG | - | 32523:36268-36287 | None:intergenic | 40.0% | |
| CTGCATAGCATAATTGGTGT+AGG | + | 32523:36851-36870 | MS.gene041630:intron | 40.0% | |
| GAATACCAAGTGTTTATGCC+GGG | - | 32523:36071-36090 | None:intergenic | 40.0% | |
| GAGATTCGTTGAGATTGGAA+GGG | + | 32523:35152-35171 | MS.gene041630:CDS | 40.0% | |
| GATCATGTTGGCAAAGATCA+AGG | + | 32523:37486-37505 | MS.gene041630:CDS | 40.0% | |
| GCATAAGCTCTTGTCAAGAT+AGG | - | 32523:36715-36734 | None:intergenic | 40.0% | |
| GGTCCCCTATAAATTTCAAG+AGG | + | 32523:37213-37232 | MS.gene041630:CDS | 40.0% | |
| TAGCATAATTGGTGTAGGGA+CGG | + | 32523:36856-36875 | MS.gene041630:intron | 40.0% | |
| !! | CAGCCCTCAATGATTTTGAT+AGG | + | 32523:37458-37477 | MS.gene041630:CDS | 40.0% |
| !! | TGTCTGCAGTGTTGATTAGT+TGG | + | 32523:36799-36818 | MS.gene041630:intron | 40.0% |
| AGGGTTGCTCTGATCAACTA+CGG | + | 32523:35171-35190 | MS.gene041630:CDS | 45.0% | |
| GTTGTGGTTTCATTGCATCC+AGG | + | 32523:37061-37080 | MS.gene041630:intron | 45.0% | |
| TATTCCCCGGCATAAACACT+TGG | + | 32523:36063-36082 | MS.gene041630:intron | 45.0% | |
| TCTTGTCAAGATAGGGCCTT+AGG | - | 32523:36707-36726 | None:intergenic | 45.0% | |
| TTGATGCTCCAGATATGGAG+AGG | + | 32523:37192-37211 | MS.gene041630:CDS | 45.0% | |
| AAGTGGGAGAAGAGCTCATG+GGG | + | 32523:37406-37425 | MS.gene041630:CDS | 50.0% | |
| ACAAGTGGGAGAAGAGCTCA+TGG | + | 32523:37404-37423 | MS.gene041630:CDS | 50.0% | |
| ATAGGGGACCTCTCCATATC+TGG | - | 32523:37203-37222 | None:intergenic | 50.0% | |
| ATCAAAGCCATGGAAGCTGC+TGG | + | 32523:37293-37312 | MS.gene041630:CDS | 50.0% | |
| CAAGTGGGAGAAGAGCTCAT+GGG | + | 32523:37405-37424 | MS.gene041630:CDS | 50.0% | |
| !! | GTGCAGTATTGGCTCAAACC+TGG | - | 32523:37082-37101 | None:intergenic | 50.0% |
| !! | GTGTAGGGACGGAAGCATTT+CGG | + | 32523:36867-36886 | MS.gene041630:intron | 50.0% |
| !! | TGTAGGGACGGAAGCATTTC+GGG | + | 32523:36868-36887 | MS.gene041630:intron | 50.0% |
| AAGCCATGGAAGCTGCTGGT+TGG | + | 32523:37297-37316 | MS.gene041630:intron | 55.0% | |
| GTACCAACCAGCAGCTTCCA+TGG | - | 32523:37303-37322 | None:intergenic | 55.0% | |
| TTACGGCTTATGCAGAGGGC+AGG | + | 32523:37582-37601 | MS.gene041630:intron | 55.0% | |
| !! | GGCAAGAGCTTGCTAAGCTG+AGG | + | 32523:37613-37632 | MS.gene041630:CDS | 55.0% |
| !! | GTAGGGACGGAAGCATTTCG+GGG | + | 32523:36869-36888 | MS.gene041630:intron | 55.0% |
| !! | TAGGGACGGAAGCATTTCGG+GGG | + | 32523:36870-36889 | MS.gene041630:intron | 55.0% |
| ! | TGCAGAGGGCAGGCGTTGTT+AGG | + | 32523:37592-37611 | MS.gene041630:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32523 | gene | 35049 | 37650 | 35049 | ID=MS.gene041630 |
| 32523 | mRNA | 35049 | 37650 | 35049 | ID=MS.gene041630.t1;Parent=MS.gene041630 |
| 32523 | exon | 35049 | 35087 | 35049 | ID=MS.gene041630.t1.exon1;Parent=MS.gene041630.t1 |
| 32523 | CDS | 35049 | 35087 | 35049 | ID=cds.MS.gene041630.t1;Parent=MS.gene041630.t1 |
| 32523 | exon | 35144 | 35245 | 35144 | ID=MS.gene041630.t1.exon2;Parent=MS.gene041630.t1 |
| 32523 | CDS | 35144 | 35245 | 35144 | ID=cds.MS.gene041630.t1;Parent=MS.gene041630.t1 |
| 32523 | exon | 37185 | 37314 | 37185 | ID=MS.gene041630.t1.exon3;Parent=MS.gene041630.t1 |
| 32523 | CDS | 37185 | 37314 | 37185 | ID=cds.MS.gene041630.t1;Parent=MS.gene041630.t1 |
| 32523 | exon | 37395 | 37507 | 37395 | ID=MS.gene041630.t1.exon4;Parent=MS.gene041630.t1 |
| 32523 | CDS | 37395 | 37507 | 37395 | ID=cds.MS.gene041630.t1;Parent=MS.gene041630.t1 |
| 32523 | exon | 37597 | 37650 | 37597 | ID=MS.gene041630.t1.exon5;Parent=MS.gene041630.t1 |
| 32523 | CDS | 37597 | 37650 | 37597 | ID=cds.MS.gene041630.t1;Parent=MS.gene041630.t1 |
| Gene Sequence |
| Protein sequence |
