Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014197.t1 | XP_020229252.1 | 91.7 | 193 | 14 | 2 | 1 | 193 | 1 | 191 | 2.10E-91 | 345.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014197.t1 | Q9LS09 | 86.4 | 169 | 22 | 1 | 1 | 168 | 1 | 169 | 3.1e-80 | 299.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014197.t1 | A0A1S3U1V4 | 95.6 | 180 | 7 | 1 | 1 | 180 | 1 | 179 | 4.3e-91 | 343.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene014197 | MS.gene94315 | PPI |
| MS.gene014197 | MS.gene66836 | PPI |
| MS.gene014197 | MS.gene066035 | PPI |
| MS.gene52970 | MS.gene014197 | PPI |
| MS.gene014301 | MS.gene014197 | PPI |
| MS.gene014197 | MS.gene069975 | PPI |
| MS.gene014197 | MS.gene52972 | PPI |
| MS.gene014197 | MS.gene71415 | PPI |
| MS.gene014197 | MS.gene066034 | PPI |
| MS.gene014197 | MS.gene94307 | PPI |
| MS.gene014197 | MS.gene004833 | PPI |
| MS.gene016712 | MS.gene014197 | PPI |
| MS.gene014197 | MS.gene041630 | PPI |
| MS.gene014197 | MS.gene064876 | PPI |
| MS.gene014197 | MS.gene81646 | PPI |
| MS.gene014197 | MS.gene98552 | PPI |
| MS.gene014197 | MS.gene070538 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014197.t1 | MTR_3g118150 | 96.373 | 193 | 7 | 0 | 1 | 193 | 1 | 193 | 5.38e-135 | 375 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014197.t1 | AT5G38110 | 86.391 | 169 | 22 | 1 | 1 | 168 | 1 | 169 | 1.13e-104 | 300 |
| MS.gene014197.t1 | AT1G66740 | 80.899 | 178 | 31 | 1 | 1 | 178 | 1 | 175 | 2.33e-103 | 296 |
Find 49 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTACTCACATGCTCTTATT+TGG | 0.139177 | 3.4:+100162568 | MS.gene014197:CDS |
| TGGATCTTGTTCTCCAGTTT+GGG | 0.231870 | 3.4:-100162795 | None:intergenic |
| GTGGATCTTGTTCTCCAGTT+TGG | 0.266545 | 3.4:-100162796 | None:intergenic |
| CTACTCACATGCTCTTATTT+GGG | 0.286175 | 3.4:+100162569 | MS.gene014197:CDS |
| GATATCTTCTTCACGAATCT+TGG | 0.287365 | 3.4:-100162528 | None:intergenic |
| TGAGACTTATGACCAGTTAT+TGG | 0.289525 | 3.4:+100162433 | MS.gene014197:CDS |
| CGAGATTTCCTATGAGTGTT+TGG | 0.353611 | 3.4:+100162075 | MS.gene014197:CDS |
| GGGTAGTTGCTCTGAGGGAG+GGG | 0.371206 | 3.4:-100162774 | None:intergenic |
| TGGGGTAGTTGCTCTGAGGG+AGG | 0.378053 | 3.4:-100162776 | None:intergenic |
| GGGGTAGTTGCTCTGAGGGA+GGG | 0.392562 | 3.4:-100162775 | None:intergenic |
| GTTCTCAGGGTGGAAATTGA+TGG | 0.403676 | 3.4:-100162732 | None:intergenic |
| GGTAGTTGCTCTGAGGGAGG+GGG | 0.407080 | 3.4:-100162773 | None:intergenic |
| CAAGGACACTCTCCAATAAC+TGG | 0.417188 | 3.4:-100162445 | None:intergenic |
| TCACGAATCTTGGATGGGTC+TGG | 0.425679 | 3.4:-100162518 | None:intergenic |
| TCAGAGCAACTACCCCAAAC+TGG | 0.436456 | 3.4:+100162782 | MS.gene014197:CDS |
| GAGAGCGGCCAAACACTCAT+AGG | 0.445137 | 3.4:-100162083 | None:intergenic |
| CTATCTGTTAAAACCTTGGT+TGG | 0.449270 | 3.4:-100162662 | None:intergenic |
| AACCCTATCTGTTAAAACCT+TGG | 0.452925 | 3.4:-100162666 | None:intergenic |
| TTATTGGAGAGTGTCCTTGT+TGG | 0.475012 | 3.4:+100162449 | MS.gene014197:CDS |
| TTCTCAGGGTGGAAATTGAT+GGG | 0.479943 | 3.4:-100162731 | None:intergenic |
| TCTGTTAAAACCTTGGTTGG+AGG | 0.483580 | 3.4:-100162659 | None:intergenic |
| GAATGGAAGCTGATTTATGT+TGG | 0.485287 | 3.4:+100162398 | MS.gene014197:CDS |
| TCTTCTTCACGAATCTTGGA+TGG | 0.488809 | 3.4:-100162524 | None:intergenic |
| ATGGGGAACTTAGTGACCCT+TGG | 0.490528 | 3.4:-100162713 | None:intergenic |
| GGTCAAGAGTTTATTCGTGT+TGG | 0.494710 | 3.4:+100162590 | MS.gene014197:CDS |
| CTTGTTGGTCCTGTCAATGT+TGG | 0.502550 | 3.4:+100162464 | MS.gene014197:CDS |
| CGATAGTTTCCAACATTGAC+AGG | 0.504203 | 3.4:-100162473 | None:intergenic |
| TAGGAAATCTCGAACTGAAA+GGG | 0.513564 | 3.4:-100162064 | None:intergenic |
| AAGGGATTGAGAAACGAAGC+TGG | 0.517879 | 3.4:-100162046 | None:intergenic |
| GTTTGGGGTAGTTGCTCTGA+GGG | 0.518854 | 3.4:-100162779 | None:intergenic |
| GAGAGAGGAACCTCCAACCA+AGG | 0.524167 | 3.4:+100162649 | MS.gene014197:CDS |
| ATTCGTGAAGAAGATATCAT+TGG | 0.528304 | 3.4:+100162533 | MS.gene014197:CDS |
| CAATTGTCACAGATTTGGAA+TGG | 0.533603 | 3.4:+100162381 | MS.gene014197:intron |
| ATAGGAAATCTCGAACTGAA+AGG | 0.536457 | 3.4:-100162065 | None:intergenic |
| CGAATCTTGGATGGGTCTGG+TGG | 0.541608 | 3.4:-100162515 | None:intergenic |
| TGTTTGGCCGCTCTCAAAGA+TGG | 0.557561 | 3.4:+100162091 | MS.gene014197:CDS |
| CTTCTTCACGAATCTTGGAT+GGG | 0.564124 | 3.4:-100162523 | None:intergenic |
| GGATCTTGTTCTCCAGTTTG+GGG | 0.567661 | 3.4:-100162794 | None:intergenic |
| AGTTTGGGGTAGTTGCTCTG+AGG | 0.582073 | 3.4:-100162780 | None:intergenic |
| GTATCGGGTGCAGCAGCAAG+TGG | 0.584533 | 3.4:-100162815 | None:intergenic |
| TGATGACGAGCAGCTGAGAG+AGG | 0.622188 | 3.4:+100162634 | MS.gene014197:CDS |
| CAACATTGACAGGACCAACA+AGG | 0.622504 | 3.4:-100162463 | None:intergenic |
| ACATCTTATCTGATAAACCA+AGG | 0.636524 | 3.4:+100162696 | MS.gene014197:CDS |
| GATTTATGTTGGATCTGCTG+AGG | 0.636648 | 3.4:+100162409 | MS.gene014197:CDS |
| GAAACGAAGCTGGATTGTCA+AGG | 0.636849 | 3.4:-100162036 | None:intergenic |
| CATCTTATCTGATAAACCAA+GGG | 0.651929 | 3.4:+100162697 | MS.gene014197:CDS |
| TCTCAGGGTGGAAATTGATG+GGG | 0.674973 | 3.4:-100162730 | None:intergenic |
| GGTTAACATCACGAACGTGA+CGG | 0.680811 | 3.4:+100162012 | MS.gene014197:CDS |
| ATACATACCATCTTTGAGAG+CGG | 0.695677 | 3.4:-100162098 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTACTCTACTTTTATTTTA+GGG | + | chr3.4:100162129-100162148 | MS.gene014197:intron | 15.0% |
| !! | AAACAAAATTAGGTCAAATT+TGG | - | chr3.4:100162236-100162255 | None:intergenic | 20.0% |
| !! | AACAAAATTAGGTCAAATTT+GGG | - | chr3.4:100162235-100162254 | None:intergenic | 20.0% |
| !! | ACTAAGTAACAAACAAAATT+AGG | - | chr3.4:100162246-100162265 | None:intergenic | 20.0% |
| !! | TTCAACTTAATAATTGCATT+GGG | - | chr3.4:100162334-100162353 | None:intergenic | 20.0% |
| !! | TTTCAACTTAATAATTGCAT+TGG | - | chr3.4:100162335-100162354 | None:intergenic | 20.0% |
| !!! | CAATTATTAAGTTGAAAATG+TGG | + | chr3.4:100162338-100162357 | MS.gene014197:intron | 20.0% |
| !!! | CTTTACTCTACTTTTATTTT+AGG | + | chr3.4:100162128-100162147 | MS.gene014197:intron | 20.0% |
| !!! | TCTACTTTCTTGTTTTTTTA+GGG | + | chr3.4:100162284-100162303 | MS.gene014197:intron | 20.0% |
| ! | CTAAAAAAACAAGAAAGTAG+AGG | - | chr3.4:100162285-100162304 | None:intergenic | 25.0% |
| ! | TCAACTTAATAATTGCATTG+GGG | - | chr3.4:100162333-100162352 | None:intergenic | 25.0% |
| !! | GTTTGTTACTTAGTTAGTTA+GGG | + | chr3.4:100162252-100162271 | MS.gene014197:intron | 25.0% |
| !! | TGTTTGTTACTTAGTTAGTT+AGG | + | chr3.4:100162251-100162270 | MS.gene014197:intron | 25.0% |
| !!! | CTCTACTTTCTTGTTTTTTT+AGG | + | chr3.4:100162283-100162302 | MS.gene014197:intron | 25.0% |
| !!! | TTCTTGTTTTTTTAGGGTTT+AGG | + | chr3.4:100162290-100162309 | MS.gene014197:intron | 25.0% |
| AAAACAAGAAAGTAGAGGAT+TGG | - | chr3.4:100162280-100162299 | None:intergenic | 30.0% | |
| AACTTAATAATTGCATTGGG+GGG | - | chr3.4:100162331-100162350 | None:intergenic | 30.0% | |
| ACATCTTATCTGATAAACCA+AGG | + | chr3.4:100162696-100162715 | MS.gene014197:CDS | 30.0% | |
| ATTCGTGAAGAAGATATCAT+TGG | + | chr3.4:100162533-100162552 | MS.gene014197:CDS | 30.0% | |
| CAACTTAATAATTGCATTGG+GGG | - | chr3.4:100162332-100162351 | None:intergenic | 30.0% | |
| CATCTTATCTGATAAACCAA+GGG | + | chr3.4:100162697-100162716 | MS.gene014197:CDS | 30.0% | |
| ! | CTTCATTTTCATTGTTCTCA+GGG | - | chr3.4:100162748-100162767 | None:intergenic | 30.0% |
| ! | TCTTCATTTTCATTGTTCTC+AGG | - | chr3.4:100162749-100162768 | None:intergenic | 30.0% |
| ! | TTTTAACAGATAGGGTTCAA+AGG | + | chr3.4:100162672-100162691 | MS.gene014197:CDS | 30.0% |
| !! | AACCAAGGTTTTAACAGATA+GGG | + | chr3.4:100162664-100162683 | MS.gene014197:CDS | 30.0% |
| !!! | AAACTATCGCTTTGTTTTAC+AGG | + | chr3.4:100162487-100162506 | MS.gene014197:CDS | 30.0% |
| AACCCTATCTGTTAAAACCT+TGG | - | chr3.4:100162669-100162688 | None:intergenic | 35.0% | |
| ATAGGAAATCTCGAACTGAA+AGG | - | chr3.4:100162068-100162087 | None:intergenic | 35.0% | |
| ATGGTCAATTGTCACAGATT+TGG | + | chr3.4:100162376-100162395 | MS.gene014197:intron | 35.0% | |
| CAATTGTCACAGATTTGGAA+TGG | + | chr3.4:100162381-100162400 | MS.gene014197:intron | 35.0% | |
| CTATCTGTTAAAACCTTGGT+TGG | - | chr3.4:100162665-100162684 | None:intergenic | 35.0% | |
| GATATCTTCTTCACGAATCT+TGG | - | chr3.4:100162531-100162550 | None:intergenic | 35.0% | |
| TAGGAAATCTCGAACTGAAA+GGG | - | chr3.4:100162067-100162086 | None:intergenic | 35.0% | |
| TCTACTCACATGCTCTTATT+TGG | + | chr3.4:100162568-100162587 | MS.gene014197:CDS | 35.0% | |
| TGAGACTTATGACCAGTTAT+TGG | + | chr3.4:100162433-100162452 | MS.gene014197:CDS | 35.0% | |
| ! | ATACATACCATCTTTGAGAG+CGG | - | chr3.4:100162101-100162120 | None:intergenic | 35.0% |
| ! | ATTTTCGTTTTCACTCTGCT+AGG | + | chr3.4:100162169-100162188 | MS.gene014197:intron | 35.0% |
| ! | CTACTCACATGCTCTTATTT+GGG | + | chr3.4:100162569-100162588 | MS.gene014197:CDS | 35.0% |
| ! | GAATGGAAGCTGATTTATGT+TGG | + | chr3.4:100162398-100162417 | MS.gene014197:CDS | 35.0% |
| ! | GTGGACTATCAATTCATTCA+TGG | + | chr3.4:100162357-100162376 | MS.gene014197:intron | 35.0% |
| !! | CAACCAAGGTTTTAACAGAT+AGG | + | chr3.4:100162663-100162682 | MS.gene014197:CDS | 35.0% |
| CGAGATTTCCTATGAGTGTT+TGG | + | chr3.4:100162075-100162094 | MS.gene014197:CDS | 40.0% | |
| CGATAGTTTCCAACATTGAC+AGG | - | chr3.4:100162476-100162495 | None:intergenic | 40.0% | |
| CTTCTTCACGAATCTTGGAT+GGG | - | chr3.4:100162526-100162545 | None:intergenic | 40.0% | |
| GATTTATGTTGGATCTGCTG+AGG | + | chr3.4:100162409-100162428 | MS.gene014197:CDS | 40.0% | |
| GGTCAAGAGTTTATTCGTGT+TGG | + | chr3.4:100162590-100162609 | MS.gene014197:CDS | 40.0% | |
| TAATAATTGCATTGGGGGGA+GGG | - | chr3.4:100162327-100162346 | None:intergenic | 40.0% | |
| TCTGTTAAAACCTTGGTTGG+AGG | - | chr3.4:100162662-100162681 | None:intergenic | 40.0% | |
| TCTTCTTCACGAATCTTGGA+TGG | - | chr3.4:100162527-100162546 | None:intergenic | 40.0% | |
| TGGATCTTGTTCTCCAGTTT+GGG | - | chr3.4:100162798-100162817 | None:intergenic | 40.0% | |
| TTAATAATTGCATTGGGGGG+AGG | - | chr3.4:100162328-100162347 | None:intergenic | 40.0% | |
| TTCTCAGGGTGGAAATTGAT+GGG | - | chr3.4:100162734-100162753 | None:intergenic | 40.0% | |
| ! | CATTTTCATTGTTCTCAGGG+TGG | - | chr3.4:100162745-100162764 | None:intergenic | 40.0% |
| ! | GTTTTCACTCTGCTAGGTTA+GGG | + | chr3.4:100162175-100162194 | MS.gene014197:intron | 40.0% |
| !! | TTATTGGAGAGTGTCCTTGT+TGG | + | chr3.4:100162449-100162468 | MS.gene014197:CDS | 40.0% |
| AAGGGATTGAGAAACGAAGC+TGG | - | chr3.4:100162049-100162068 | None:intergenic | 45.0% | |
| AATAATTGCATTGGGGGGAG+GGG | - | chr3.4:100162326-100162345 | None:intergenic | 45.0% | |
| ACTCTGCTAGGTTAGGGTTT+AGG | + | chr3.4:100162181-100162200 | MS.gene014197:intron | 45.0% | |
| ATACCATTCCCCCAAATGAG+GGG | + | chr3.4:100162834-100162853 | MS.gene014197:CDS | 45.0% | |
| CAACATTGACAGGACCAACA+AGG | - | chr3.4:100162466-100162485 | None:intergenic | 45.0% | |
| CAAGGACACTCTCCAATAAC+TGG | - | chr3.4:100162448-100162467 | None:intergenic | 45.0% | |
| CTCATTTGGGGGAATGGTAT+CGG | - | chr3.4:100162834-100162853 | None:intergenic | 45.0% | |
| CTCTGCTAGGTTAGGGTTTA+GGG | + | chr3.4:100162182-100162201 | MS.gene014197:intron | 45.0% | |
| CTTGTTGGTCCTGTCAATGT+TGG | + | chr3.4:100162464-100162483 | MS.gene014197:CDS | 45.0% | |
| GAAACGAAGCTGGATTGTCA+AGG | - | chr3.4:100162039-100162058 | None:intergenic | 45.0% | |
| GATACCATTCCCCCAAATGA+GGG | + | chr3.4:100162833-100162852 | MS.gene014197:CDS | 45.0% | |
| GGATCTTGTTCTCCAGTTTG+GGG | - | chr3.4:100162797-100162816 | None:intergenic | 45.0% | |
| GGTTAACATCACGAACGTGA+CGG | + | chr3.4:100162012-100162031 | MS.gene014197:CDS | 45.0% | |
| GTGGATCTTGTTCTCCAGTT+TGG | - | chr3.4:100162799-100162818 | None:intergenic | 45.0% | |
| GTTCTCAGGGTGGAAATTGA+TGG | - | chr3.4:100162735-100162754 | None:intergenic | 45.0% | |
| TCATTTGGGGGAATGGTATC+GGG | - | chr3.4:100162833-100162852 | None:intergenic | 45.0% | |
| TCTCAGGGTGGAAATTGATG+GGG | - | chr3.4:100162733-100162752 | None:intergenic | 45.0% | |
| TTAAGAACCCCCCTCATTTG+GGG | - | chr3.4:100162846-100162865 | None:intergenic | 45.0% | |
| ! | CGTTTTCACTCTGCTAGGTT+AGG | + | chr3.4:100162174-100162193 | MS.gene014197:intron | 45.0% |
| ATGGGGAACTTAGTGACCCT+TGG | - | chr3.4:100162716-100162735 | None:intergenic | 50.0% | |
| CGATACCATTCCCCCAAATG+AGG | + | chr3.4:100162832-100162851 | MS.gene014197:CDS | 50.0% | |
| TAAGAACCCCCCTCATTTGG+GGG | - | chr3.4:100162845-100162864 | None:intergenic | 50.0% | |
| TACCATTCCCCCAAATGAGG+GGG | + | chr3.4:100162835-100162854 | MS.gene014197:CDS | 50.0% | |
| TCACGAATCTTGGATGGGTC+TGG | - | chr3.4:100162521-100162540 | None:intergenic | 50.0% | |
| TCAGAGCAACTACCCCAAAC+TGG | + | chr3.4:100162782-100162801 | MS.gene014197:CDS | 50.0% | |
| TGTTTGGCCGCTCTCAAAGA+TGG | + | chr3.4:100162091-100162110 | MS.gene014197:CDS | 50.0% | |
| ! | AGTTTGGGGTAGTTGCTCTG+AGG | - | chr3.4:100162783-100162802 | None:intergenic | 50.0% |
| ! | GTTTGGGGTAGTTGCTCTGA+GGG | - | chr3.4:100162782-100162801 | None:intergenic | 50.0% |
| ACCATTCCCCCAAATGAGGG+GGG | + | chr3.4:100162836-100162855 | MS.gene014197:CDS | 55.0% | |
| CGAATCTTGGATGGGTCTGG+TGG | - | chr3.4:100162518-100162537 | None:intergenic | 55.0% | |
| GAGAGAGGAACCTCCAACCA+AGG | + | chr3.4:100162649-100162668 | MS.gene014197:CDS | 55.0% | |
| GAGAGCGGCCAAACACTCAT+AGG | - | chr3.4:100162086-100162105 | None:intergenic | 55.0% | |
| ! | TGATGACGAGCAGCTGAGAG+AGG | + | chr3.4:100162634-100162653 | MS.gene014197:CDS | 55.0% |
| ACCCCCCTCATTTGGGGGAA+TGG | - | chr3.4:100162840-100162859 | None:intergenic | 60.0% | |
| GTATCGGGTGCAGCAGCAAG+TGG | - | chr3.4:100162818-100162837 | None:intergenic | 60.0% | |
| !! | GGGGTAGTTGCTCTGAGGGA+GGG | - | chr3.4:100162778-100162797 | None:intergenic | 60.0% |
| !! | GGGTAGTTGCTCTGAGGGAG+GGG | - | chr3.4:100162777-100162796 | None:intergenic | 60.0% |
| !! | GGTAGTTGCTCTGAGGGAGG+GGG | - | chr3.4:100162776-100162795 | None:intergenic | 60.0% |
| !! | TGGGGTAGTTGCTCTGAGGG+AGG | - | chr3.4:100162779-100162798 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 100162004 | 100162865 | 100162004 | ID=MS.gene014197 |
| chr3.4 | mRNA | 100162004 | 100162865 | 100162004 | ID=MS.gene014197.t1;Parent=MS.gene014197 |
| chr3.4 | exon | 100162004 | 100162112 | 100162004 | ID=MS.gene014197.t1.exon1;Parent=MS.gene014197.t1 |
| chr3.4 | CDS | 100162004 | 100162112 | 100162004 | ID=cds.MS.gene014197.t1;Parent=MS.gene014197.t1 |
| chr3.4 | exon | 100162393 | 100162865 | 100162393 | ID=MS.gene014197.t1.exon2;Parent=MS.gene014197.t1 |
| chr3.4 | CDS | 100162393 | 100162865 | 100162393 | ID=cds.MS.gene014197.t1;Parent=MS.gene014197.t1 |
| Gene Sequence |
| Protein sequence |
