Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005619.t1 | XP_013469188.1 | 92.5 | 214 | 16 | 0 | 1 | 214 | 1 | 214 | 9.30E-109 | 402.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005619.t1 | P46423 | 66.0 | 206 | 70 | 0 | 4 | 209 | 3 | 208 | 4.9e-74 | 278.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005619.t1 | A0A072VNK7 | 92.5 | 214 | 16 | 0 | 1 | 214 | 1 | 214 | 6.7e-109 | 402.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene20712 | MS.gene005619 | PPI |
| MS.gene005619 | MS.gene07192 | PPI |
| MS.gene03035 | MS.gene005619 | PPI |
| MS.gene20711 | MS.gene005619 | PPI |
| MS.gene07195 | MS.gene005619 | PPI |
| MS.gene07174 | MS.gene005619 | PPI |
| MS.gene07196 | MS.gene005619 | PPI |
| MS.gene005619 | MS.gene03036 | PPI |
| MS.gene03034 | MS.gene005619 | PPI |
| MS.gene005619 | MS.gene20713 | PPI |
| MS.gene005620 | MS.gene005619 | PPI |
| MS.gene005621 | MS.gene005619 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005619.t1 | MTR_1g088840 | 92.523 | 214 | 16 | 0 | 1 | 214 | 1 | 214 | 1.65e-149 | 414 |
| MS.gene005619.t1 | MTR_1g088825 | 92.523 | 214 | 16 | 0 | 1 | 214 | 1 | 214 | 1.65e-149 | 414 |
| MS.gene005619.t1 | MTR_1g088845 | 86.916 | 214 | 28 | 0 | 1 | 214 | 1 | 214 | 1.55e-139 | 389 |
| MS.gene005619.t1 | MTR_1g088840 | 91.566 | 166 | 14 | 0 | 49 | 214 | 2 | 167 | 3.83e-112 | 318 |
| MS.gene005619.t1 | MTR_1g088840 | 92.373 | 118 | 9 | 0 | 97 | 214 | 1 | 118 | 3.62e-77 | 228 |
| MS.gene005619.t1 | MTR_3g064700 | 46.117 | 206 | 110 | 1 | 4 | 208 | 3 | 208 | 3.00e-60 | 188 |
| MS.gene005619.t1 | MTR_3g450930 | 44.498 | 209 | 114 | 2 | 4 | 211 | 3 | 210 | 4.70e-59 | 185 |
| MS.gene005619.t1 | MTR_3g450790 | 44.019 | 209 | 115 | 2 | 4 | 211 | 3 | 210 | 3.63e-58 | 182 |
| MS.gene005619.t1 | MTR_1g088850 | 39.713 | 209 | 125 | 1 | 3 | 210 | 2 | 210 | 1.57e-56 | 179 |
| MS.gene005619.t1 | MTR_5g090920 | 44.019 | 209 | 112 | 4 | 4 | 209 | 3 | 209 | 8.61e-56 | 177 |
| MS.gene005619.t1 | MTR_1g026140 | 42.130 | 216 | 119 | 3 | 4 | 213 | 5 | 220 | 2.46e-55 | 176 |
| MS.gene005619.t1 | MTR_5g090910 | 41.905 | 210 | 119 | 3 | 4 | 211 | 3 | 211 | 1.30e-54 | 174 |
| MS.gene005619.t1 | MTR_1g492670 | 86.765 | 68 | 9 | 0 | 147 | 214 | 2 | 69 | 6.11e-39 | 129 |
| MS.gene005619.t1 | MTR_5g090910 | 40.136 | 147 | 86 | 2 | 67 | 211 | 8 | 154 | 3.59e-32 | 114 |
| MS.gene005619.t1 | MTR_3g088635 | 23.013 | 239 | 152 | 5 | 4 | 210 | 1 | 239 | 2.96e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005619.t1 | AT1G02920 | 63.158 | 209 | 77 | 0 | 1 | 209 | 1 | 209 | 5.04e-89 | 261 |
| MS.gene005619.t1 | AT1G02930 | 61.722 | 209 | 79 | 1 | 1 | 209 | 1 | 208 | 6.37e-88 | 258 |
| MS.gene005619.t1 | AT1G02930 | 61.722 | 209 | 79 | 1 | 1 | 209 | 1 | 208 | 6.37e-88 | 258 |
| MS.gene005619.t1 | AT2G47730 | 58.049 | 205 | 85 | 1 | 6 | 209 | 54 | 258 | 3.82e-86 | 256 |
| MS.gene005619.t1 | AT2G47730 | 58.049 | 205 | 85 | 1 | 6 | 209 | 54 | 258 | 3.82e-86 | 256 |
| MS.gene005619.t1 | AT4G02520 | 59.242 | 211 | 83 | 1 | 1 | 208 | 1 | 211 | 6.27e-85 | 251 |
| MS.gene005619.t1 | AT2G02930 | 58.962 | 212 | 84 | 1 | 1 | 209 | 1 | 212 | 3.66e-83 | 246 |
| MS.gene005619.t1 | AT1G02950 | 54.369 | 206 | 93 | 1 | 1 | 205 | 24 | 229 | 5.48e-78 | 234 |
| MS.gene005619.t1 | AT1G02950 | 54.950 | 202 | 90 | 1 | 5 | 205 | 26 | 227 | 2.61e-77 | 232 |
| MS.gene005619.t1 | AT1G02950 | 54.950 | 202 | 90 | 1 | 5 | 205 | 26 | 227 | 2.61e-77 | 232 |
| MS.gene005619.t1 | AT1G02950 | 54.950 | 202 | 90 | 1 | 5 | 205 | 38 | 239 | 2.86e-77 | 233 |
| MS.gene005619.t1 | AT1G02940 | 47.087 | 206 | 108 | 1 | 5 | 209 | 40 | 245 | 2.56e-66 | 205 |
| MS.gene005619.t1 | AT1G02940 | 47.087 | 206 | 108 | 1 | 5 | 209 | 40 | 245 | 2.56e-66 | 205 |
| MS.gene005619.t1 | AT1G02940 | 47.087 | 206 | 108 | 1 | 5 | 209 | 65 | 270 | 3.54e-66 | 205 |
| MS.gene005619.t1 | AT1G02940 | 47.087 | 206 | 108 | 1 | 5 | 209 | 53 | 258 | 4.34e-66 | 205 |
| MS.gene005619.t1 | AT3G62760 | 47.826 | 207 | 107 | 1 | 3 | 208 | 2 | 208 | 2.80e-63 | 196 |
| MS.gene005619.t1 | AT1G49860 | 47.692 | 195 | 98 | 2 | 21 | 211 | 22 | 216 | 7.64e-58 | 183 |
| MS.gene005619.t1 | AT3G03190 | 44.498 | 209 | 115 | 1 | 4 | 211 | 3 | 211 | 5.66e-57 | 180 |
| MS.gene005619.t1 | AT5G17220 | 39.234 | 209 | 126 | 1 | 4 | 211 | 3 | 211 | 6.49e-52 | 167 |
| MS.gene005619.t1 | AT2G30860 | 40.385 | 208 | 122 | 2 | 4 | 210 | 3 | 209 | 5.72e-50 | 162 |
| MS.gene005619.t1 | AT2G30870 | 38.424 | 203 | 124 | 1 | 9 | 210 | 7 | 209 | 2.74e-46 | 152 |
| MS.gene005619.t1 | AT1G02950 | 50.704 | 142 | 69 | 1 | 65 | 205 | 60 | 201 | 4.50e-44 | 147 |
| MS.gene005619.t1 | AT2G30860 | 37.500 | 152 | 94 | 1 | 4 | 155 | 3 | 153 | 5.86e-31 | 112 |
| MS.gene005619.t1 | AT5G41210 | 25.980 | 204 | 145 | 2 | 1 | 198 | 1 | 204 | 1.45e-11 | 62.4 |
| MS.gene005619.t1 | AT5G41240 | 29.208 | 202 | 131 | 5 | 4 | 196 | 3 | 201 | 3.80e-11 | 62.4 |
Find 0 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATACATTGATAATTTAACAT+TGG | + | 8682:60792-60811 | None:intergenic | 15.0% |
| ! | AAAATTGAAATGCAGAATCA+AGG | - | 8682:60897-60916 | MS.gene005619:intron | 25.0% |
| ! | AAATTGAAATGCAGAATCAA+GGG | - | 8682:60898-60917 | MS.gene005619:intron | 25.0% |
| ! | TTCATCAAGTAATGAAAAGT+AGG | + | 8682:61229-61248 | None:intergenic | 25.0% |
| !! | ATCAATTAGTTTGATCAATG+TGG | + | 8682:60578-60597 | None:intergenic | 25.0% |
| !! | GTTAACATTAACTTGAAGAA+TGG | - | 8682:60401-60420 | MS.gene005619:CDS | 25.0% |
| !! | TCATTCTCTTCAACAATTTT+TGG | + | 8682:61106-61125 | None:intergenic | 25.0% |
| AACAATGAGTATGCTGATAA+GGG | - | 8682:60938-60957 | MS.gene005619:intron | 30.0% | |
| ATCAAGAATGCTATCCAATT+TGG | + | 8682:61131-61150 | None:intergenic | 30.0% | |
| CTTACATTGAGTGATATGAA+GGG | + | 8682:60443-60462 | None:intergenic | 30.0% | |
| TAACAATGAGTATGCTGATA+AGG | - | 8682:60937-60956 | MS.gene005619:intron | 30.0% | |
| TCCAAGAAGATAGCAATAAT+GGG | - | 8682:60983-61002 | MS.gene005619:intron | 30.0% | |
| TCGTGATTCAAACAATTTCT+TGG | + | 8682:61260-61279 | None:intergenic | 30.0% | |
| TTGGGGATAAAGAGTTTATT+TGG | - | 8682:61067-61086 | MS.gene005619:intron | 30.0% | |
| ! | AAGTAGCATCAAAACTTGTT+CGG | - | 8682:61041-61060 | MS.gene005619:intron | 30.0% |
| ! | ATTAGTTTGATCAATGTGGT+TGG | + | 8682:60574-60593 | None:intergenic | 30.0% |
| ! | GAGTTTATTTGGAATTCCTT+TGG | - | 8682:61078-61097 | MS.gene005619:intron | 30.0% |
| !! | TCAACAATTTTTGGATCCAA+AGG | + | 8682:61097-61116 | None:intergenic | 30.0% |
| !!! | GTTTCTTTTCATTTTTTGCG+AGG | + | 8682:60749-60768 | None:intergenic | 30.0% |
| AAATCTCCATCTTCAAAAGC+TGG | + | 8682:60634-60653 | None:intergenic | 35.0% | |
| ACAATGAGTATGCTGATAAG+GGG | - | 8682:60939-60958 | MS.gene005619:intron | 35.0% | |
| ATTGTCTGAGTCCAAATACT+TGG | - | 8682:61165-61184 | MS.gene005619:intron | 35.0% | |
| CAAGAAGATAGCAATAATGG+GGG | - | 8682:60985-61004 | MS.gene005619:intron | 35.0% | |
| CCAAGAAGATAGCAATAATG+GGG | - | 8682:60984-61003 | MS.gene005619:intron | 35.0% | |
| CTCCAAGAAGATAGCAATAA+TGG | - | 8682:60982-61001 | MS.gene005619:intron | 35.0% | |
| GAAGAATGGAGAACACAAAA+AGG | - | 8682:60415-60434 | MS.gene005619:CDS | 35.0% | |
| GCTTACATTGAGTGATATGA+AGG | + | 8682:60444-60463 | None:intergenic | 35.0% | |
| TAAATGATGCAAATCCACCA+AGG | + | 8682:61206-61225 | None:intergenic | 35.0% | |
| TGAAGAGAATGAAGCCAAAT+TGG | - | 8682:61114-61133 | MS.gene005619:intron | 35.0% | |
| TTCTTTATGTTGCAGCCATT+TGG | - | 8682:60601-60620 | MS.gene005619:CDS | 35.0% | |
| TTGTCTGAGTCCAAATACTT+GGG | - | 8682:61166-61185 | MS.gene005619:intron | 35.0% | |
| ! | AGTAGCATCAAAACTTGTTC+GGG | - | 8682:61042-61061 | MS.gene005619:intron | 35.0% |
| ! | ATCAAAACTTGTTCGGGAAT+TGG | - | 8682:61048-61067 | MS.gene005619:intron | 35.0% |
| ! | ATGGCATCAATAAAAGTGCA+CGG | - | 8682:60308-60327 | MS.gene005619:CDS | 35.0% |
| ! | GATGGAGATTTAAAGCTCTT+TGG | - | 8682:60643-60662 | MS.gene005619:CDS | 35.0% |
| ! | TCAAAACTTGTTCGGGAATT+GGG | - | 8682:61049-61068 | MS.gene005619:intron | 35.0% |
| ! | TTTTTGCGAGGAGTGAAATT+AGG | + | 8682:60737-60756 | None:intergenic | 35.0% |
| AAAGCTGGAACTTGACCAAA+TGG | + | 8682:60619-60638 | None:intergenic | 40.0% | |
| ACGATCTCATGTTAGTGCTT+GGG | - | 8682:61276-61295 | MS.gene005619:CDS | 40.0% | |
| CCCCATTATTGCTATCTTCT+TGG | + | 8682:60987-61006 | None:intergenic | 40.0% | |
| TCAATGTGGTTGGAACAAGA+AGG | + | 8682:60564-60583 | None:intergenic | 40.0% | |
| ! | AGATAGCAATAATGGGGGTA+TGG | - | 8682:60990-61009 | MS.gene005619:intron | 40.0% |
| ! | ATGCTACTTGATCGAAGTGT+TGG | + | 8682:61030-61049 | None:intergenic | 40.0% |
| ! | CAAAACTTGTTCGGGAATTG+GGG | - | 8682:61050-61069 | MS.gene005619:intron | 40.0% |
| ! | GGAGTCATTGCTTGTTAACT+TGG | + | 8682:60966-60985 | None:intergenic | 40.0% |
| !! | CAAGTTCCAGCTTTTGAAGA+TGG | - | 8682:60625-60644 | MS.gene005619:CDS | 40.0% |
| AGAGTCACCTCCCAAGTATT+TGG | + | 8682:61179-61198 | None:intergenic | 45.0% | |
| ATATCACTGCAAGACCAGCT+TGG | - | 8682:61305-61324 | MS.gene005619:CDS | 45.0% | |
| ATCTCATGTTAGTGCTTGGG+TGG | - | 8682:61279-61298 | MS.gene005619:CDS | 45.0% | |
| CACGATCTCATGTTAGTGCT+TGG | - | 8682:61275-61294 | MS.gene005619:CDS | 45.0% | |
| CTAATATTTGTGCGAGCGCT+TGG | - | 8682:60845-60864 | MS.gene005619:intron | 45.0% | |
| TCTGAGTCCAAATACTTGGG+AGG | - | 8682:61169-61188 | MS.gene005619:intron | 45.0% | |
| AGGTGACTCTTTCACCTTGG+TGG | - | 8682:61189-61208 | MS.gene005619:intron | 50.0% | |
| GCAAGGACTTTAGACCAAGC+TGG | + | 8682:61322-61341 | None:intergenic | 50.0% | |
| ! | AATGGGGGTATGGTCTGAAG+TGG | - | 8682:61000-61019 | MS.gene005619:intron | 50.0% |
| AGTTGCAGTAACCCGCATGG+TGG | + | 8682:60351-60370 | None:intergenic | 55.0% | |
| GAGAGTTGCAGTAACCCGCA+TGG | + | 8682:60354-60373 | None:intergenic | 55.0% | |
| GGGAGGTGACTCTTTCACCT+TGG | - | 8682:61186-61205 | MS.gene005619:intron | 55.0% | |
| CCTACTCAACGGCCACCATG+CGG | - | 8682:60336-60355 | MS.gene005619:CDS | 60.0% | |
| CGCATGGTGGCCGTTGAGTA+GGG | + | 8682:60338-60357 | None:intergenic | 60.0% | |
| CTACTCAACGGCCACCATGC+GGG | - | 8682:60337-60356 | MS.gene005619:CDS | 60.0% | |
| GCACGGAAGCCCCTACTCAA+CGG | - | 8682:60325-60344 | MS.gene005619:CDS | 60.0% | |
| GCATGGTGGCCGTTGAGTAG+GGG | + | 8682:60337-60356 | None:intergenic | 60.0% | |
| CCGCATGGTGGCCGTTGAGT+AGG | + | 8682:60339-60358 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8682 | gene | 60308 | 61357 | 60308 | ID=MS.gene005619 |
| 8682 | mRNA | 60308 | 61357 | 60308 | ID=MS.gene005619.t1;Parent=MS.gene005619 |
| 8682 | exon | 61208 | 61357 | 61208 | ID=MS.gene005619.t1.exon1;Parent=MS.gene005619.t1 |
| 8682 | CDS | 61208 | 61357 | 61208 | ID=cds.MS.gene005619.t1;Parent=MS.gene005619.t1 |
| 8682 | exon | 61001 | 61049 | 61001 | ID=MS.gene005619.t1.exon2;Parent=MS.gene005619.t1 |
| 8682 | CDS | 61001 | 61049 | 61001 | ID=cds.MS.gene005619.t1;Parent=MS.gene005619.t1 |
| 8682 | exon | 60308 | 60753 | 60308 | ID=MS.gene005619.t1.exon3;Parent=MS.gene005619.t1 |
| 8682 | CDS | 60308 | 60753 | 60308 | ID=cds.MS.gene005619.t1;Parent=MS.gene005619.t1 |
| Gene Sequence |
| Protein sequence |
