AlfalfaGEDB | MS.gene005621

M. sativa cultivar XinJiangDaYe / MS.gene005621

KEGG: K00799
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00043.28 PF02798.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005621.t1	XP_013469193.1	97.3	220	6	0	1	220	1	220	2.00E-119	438.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005621.t1	Q9LZI9	57.3	213	91	0	1	213	1	213	1.2e-72	274.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005621.t1	A0A072VNL4	97.3	220	6	0	1	220	1	220	1.5e-119	438.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene005621	MS.gene068679	PPI
MS.gene005621	MS.gene03036	PPI
MS.gene005621	MS.gene061566	PPI
MS.gene04976	MS.gene005621	PPI
MS.gene062848	MS.gene005621	PPI
MS.gene062847	MS.gene005621	PPI
MS.gene005621	MS.gene22188	PPI
MS.gene065582	MS.gene005621	PPI
MS.gene005621	MS.gene83384	PPI
MS.gene005621	MS.gene20713	PPI
MS.gene062849	MS.gene005621	PPI
MS.gene005621	MS.gene47714	PPI
MS.gene005621	MS.gene48030	PPI
MS.gene005621	MS.gene039175	PPI
MS.gene005621	MS.gene049706	PPI
MS.gene005621	MS.gene017062	PPI
MS.gene005621	MS.gene05016	PPI
MS.gene03035	MS.gene005621	PPI
MS.gene005621	MS.gene008334	PPI
MS.gene008337	MS.gene005621	PPI
MS.gene005621	MS.gene039177	PPI
MS.gene005621	MS.gene005620	PPI
MS.gene005621	MS.gene048703	PPI
MS.gene005621	MS.gene063436	PPI
MS.gene038277	MS.gene005621	PPI
MS.gene005621	MS.gene063433	PPI
MS.gene062850	MS.gene005621	PPI
MS.gene008278	MS.gene005621	PPI
MS.gene005621	MS.gene065585	PPI
MS.gene005621	MS.gene062845	PPI
MS.gene005621	MS.gene063431	PPI
MS.gene005621	MS.gene048743	PPI
MS.gene005621	MS.gene69499	PPI
MS.gene005621	MS.gene039178	PPI
MS.gene065586	MS.gene005621	PPI
MS.gene005621	MS.gene063432	PPI
MS.gene07195	MS.gene005621	PPI
MS.gene005621	MS.gene48032	PPI
MS.gene005621	MS.gene039174	PPI
MS.gene005621	MS.gene039181	PPI
MS.gene005621	MS.gene69505	PPI
MS.gene005621	MS.gene30529	PPI
MS.gene065587	MS.gene005621	PPI
MS.gene07196	MS.gene005621	PPI
MS.gene005621	MS.gene07174	PPI
MS.gene017060	MS.gene005621	PPI
MS.gene005621	MS.gene005619	PPI
MS.gene005621	MS.gene07196	PPI
MS.gene005621	MS.gene20712	PPI
MS.gene005621	MS.gene019181	PPI
MS.gene005621	MS.gene063430	PPI
MS.gene005621	MS.gene062844	PPI
MS.gene005621	MS.gene22195	PPI
MS.gene019183	MS.gene005621	PPI
MS.gene005621	MS.gene039180	PPI
MS.gene005621	MS.gene03035	PPI
MS.gene063437	MS.gene005621	PPI
MS.gene005621	MS.gene068678	PPI
MS.gene000510	MS.gene005621	PPI
MS.gene005621	MS.gene039179	PPI
MS.gene005621	MS.gene049699	PPI
MS.gene005621	MS.gene22196	PPI
MS.gene068681	MS.gene005621	PPI
MS.gene005621	MS.gene062846	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005621.t1	MTR_1g088850	97.273	220	6	0	1	220	1	220	2.00e-163	449
MS.gene005621.t1	MTR_3g450790	48.571	210	105	3	1	209	1	208	1.15e-69	212
MS.gene005621.t1	MTR_3g450930	46.190	210	110	3	1	209	1	208	1.31e-63	197
MS.gene005621.t1	MTR_1g088845	41.346	208	121	1	3	210	4	210	7.02e-58	182
MS.gene005621.t1	MTR_5g090920	44.550	211	113	4	1	209	1	209	1.08e-57	182
MS.gene005621.t1	MTR_1g088840	39.904	208	124	1	3	210	4	210	8.38e-57	179
MS.gene005621.t1	MTR_1g088825	39.904	208	124	1	3	210	4	210	8.38e-57	179
MS.gene005621.t1	MTR_3g064700	43.062	209	117	2	1	208	1	208	6.41e-56	177
MS.gene005621.t1	MTR_5g090910	42.925	212	115	5	1	209	1	209	1.91e-55	176
MS.gene005621.t1	MTR_1g026140	37.264	212	128	1	3	209	5	216	3.21e-50	163
MS.gene005621.t1	MTR_1g088840	39.506	162	97	1	49	210	3	163	5.27e-41	137
MS.gene005621.t1	MTR_5g090910	41.497	147	81	4	66	209	8	152	2.12e-29	107
MS.gene005621.t1	MTR_1g088840	37.500	112	70	0	99	210	3	114	1.69e-24	94.0
MS.gene005621.t1	MTR_4g134770	26.316	228	144	5	1	219	1	213	3.51e-14	70.9
MS.gene005621.t1	MTR_3g058940	27.830	212	142	5	1	210	1	203	1.45e-13	69.3
MS.gene005621.t1	MTR_1g492670	42.188	64	37	0	147	210	2	65	2.83e-12	60.5
MS.gene005621.t1	MTR_2g005570	27.053	207	130	5	11	210	13	205	6.61e-12	64.3
MS.gene005621.t1	MTR_2g005570	27.184	206	131	5	11	210	13	205	9.29e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene005621.t1	AT3G62760	56.221	217	95	0	1	217	1	217	9.62e-96	278
MS.gene005621.t1	AT1G02930	42.308	208	118	1	2	209	3	208	1.24e-59	186
MS.gene005621.t1	AT1G02930	42.308	208	118	1	2	209	3	208	1.24e-59	186
MS.gene005621.t1	AT1G02920	41.827	208	120	1	2	209	3	209	1.29e-57	181
MS.gene005621.t1	AT2G30860	44.186	215	117	3	1	214	1	213	2.68e-56	178
MS.gene005621.t1	AT2G47730	42.439	205	118	0	5	209	54	258	7.82e-56	179
MS.gene005621.t1	AT2G47730	42.439	205	118	0	5	209	54	258	7.82e-56	179
MS.gene005621.t1	AT4G02520	40.952	210	120	2	2	208	3	211	6.89e-55	174
MS.gene005621.t1	AT2G30870	44.019	209	114	3	1	208	1	207	3.57e-54	173
MS.gene005621.t1	AT2G02930	40.000	210	124	1	2	209	3	212	5.67e-54	172
MS.gene005621.t1	AT1G02950	35.922	206	132	0	1	206	23	228	2.14e-50	164
MS.gene005621.t1	AT1G02950	35.922	206	132	0	1	206	23	228	2.14e-50	164
MS.gene005621.t1	AT1G02950	35.922	206	132	0	1	206	35	240	2.67e-50	164
MS.gene005621.t1	AT1G02950	36.453	203	129	0	4	206	28	230	3.27e-50	164
MS.gene005621.t1	AT3G03190	37.619	210	129	2	1	209	1	209	6.50e-49	159
MS.gene005621.t1	AT1G49860	41.500	200	112	3	14	209	16	214	1.19e-47	157
MS.gene005621.t1	AT1G02940	36.893	206	130	0	4	209	40	245	9.14e-47	155
MS.gene005621.t1	AT1G02940	36.893	206	130	0	4	209	40	245	9.14e-47	155
MS.gene005621.t1	AT1G02940	36.893	206	130	0	4	209	53	258	1.36e-46	155
MS.gene005621.t1	AT1G02940	36.893	206	130	0	4	209	65	270	1.38e-46	155
MS.gene005621.t1	AT5G17220	33.333	210	138	2	1	209	1	209	1.05e-45	151
MS.gene005621.t1	AT2G30860	40.645	155	90	2	1	155	1	153	4.31e-33	117
MS.gene005621.t1	AT1G02950	29.143	175	120	1	32	206	32	202	6.14e-26	100
MS.gene005621.t1	AT1G09640	26.415	212	146	5	1	210	1	204	1.20e-11	63.5
MS.gene005621.t1	AT1G57720	26.606	218	138	7	1	210	1	204	1.26e-11	63.5
MS.gene005621.t1	AT1G57720	26.606	218	138	7	1	210	1	204	1.26e-11	63.5
MS.gene005621.t1	AT1G09640	26.415	212	146	5	1	210	1	204	1.58e-11	62.4

Find 0 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTCGAATAAACTTAAA+GGG	-	8682:77871-77890	None:intergenic	15.0%
!!!	TATTTTTCGAATAAACTTAA+AGG	-	8682:77872-77891	None:intergenic	15.0%
!!	AATTATCTATGAATACTTTG+TGG	+	8682:78295-78314	MS.gene005621:CDS	20.0%
!!	GAATTAGTAACAAAAATTAG+AGG	-	8682:78004-78023	None:intergenic	20.0%
!!!	ACAAAGTATTCATAGATAAT+TGG	-	8682:78296-78315	None:intergenic	20.0%
!	AATAGGTTACATATCATTAC+AGG	-	8682:78102-78121	None:intergenic	25.0%
!	AATCAAGCAAAAATACTACT+AGG	+	8682:77919-77938	MS.gene005621:intron	25.0%
!	ATATGTAGCTGAAAAATACA+AGG	+	8682:78169-78188	MS.gene005621:CDS	25.0%
!	ATCATTACAGGTTCAAAAAA+TGG	-	8682:78090-78109	None:intergenic	25.0%
!!	TAACCTATTTTATGTGACTA+TGG	+	8682:78113-78132	MS.gene005621:intron	25.0%
!!	TTTTTCATGTAGACATATCA+TGG	-	8682:77596-77615	None:intergenic	25.0%
	AGTCCATAGTCACATAAAAT+AGG	-	8682:78119-78138	None:intergenic	30.0%
	GCTGAAAAATACAAGGAAAA+TGG	+	8682:78176-78195	MS.gene005621:CDS	30.0%
	TTTCGAATAAACTTAAAGGG+TGG	-	8682:77868-77887	None:intergenic	30.0%
!	AAAAAGAAAGCATACCAAAG+AGG	-	8682:77810-77829	None:intergenic	30.0%
!	AAAAGAAAGCATACCAAAGA+GGG	-	8682:77809-77828	None:intergenic	30.0%
!	AAAATGGACCTGATTTGATT+AGG	+	8682:78192-78211	MS.gene005621:CDS	30.0%
!	AATAGTGAGTTGAAGATATG+TGG	-	8682:78463-78482	None:intergenic	30.0%
!!	GTATTTTTCAGCTACATATG+AGG	-	8682:78168-78187	None:intergenic	30.0%
	AAGCTTCATTGGTCAAAATG+TGG	+	8682:78231-78250	MS.gene005621:CDS	35.0%
	AGATCATCATCTTCAAGTAC+TGG	-	8682:77784-77803	None:intergenic	35.0%
	ATTGATCATTGAAGCACAAG+GGG	-	8682:78495-78514	None:intergenic	35.0%
	CATTGATCATTGAAGCACAA+GGG	-	8682:78496-78515	None:intergenic	35.0%
	CTTACGTTCTTGTTGAGAAA+AGG	-	8682:77676-77695	None:intergenic	35.0%
	GTGCTTCAATGATCAATGAA+CGG	+	8682:78498-78517	MS.gene005621:CDS	35.0%
	TACTTTGTGGTTCCATTTCA+AGG	+	8682:78308-78327	MS.gene005621:CDS	35.0%
	TCATTGATCATTGAAGCACA+AGG	-	8682:78497-78516	None:intergenic	35.0%
	TCATTGTGCCTAATCAAATC+AGG	-	8682:78203-78222	None:intergenic	35.0%
	TCTGCTTTCCAAAAAGTTGT+GGG	+	8682:78560-78579	MS.gene005621:CDS	35.0%
	TGACACAAAAGAAGCTTCAT+TGG	+	8682:78220-78239	MS.gene005621:CDS	35.0%
	TTCAGCTACATATGAGGTTA+TGG	-	8682:78162-78181	None:intergenic	35.0%
!	AGTACTGGAATCAAACCAAA+GGG	-	8682:77769-77788	None:intergenic	35.0%
!	CAATGAACGGATTCATGTTA+AGG	+	8682:78511-78530	MS.gene005621:CDS	35.0%
!	TTTTTGATCACTCTGACCAA+AGG	-	8682:78594-78613	None:intergenic	35.0%
!!	AAGTACTGGAATCAAACCAA+AGG	-	8682:77770-77789	None:intergenic	35.0%
!!	GTATTTTGTTGTGCTTAGCT+TGG	-	8682:78405-78424	None:intergenic	35.0%
	ACGTTCTTGTTGAGAAAAGG+AGG	-	8682:77673-77692	None:intergenic	40.0%
	AGATAATTGGTGTGACTGCA+GGG	-	8682:78283-78302	None:intergenic	40.0%
	AGCACAACAAAATACCTTGC+AGG	+	8682:78410-78429	MS.gene005621:CDS	40.0%
	ATTGGTCAAAATGTGGACAG+AGG	+	8682:78238-78257	MS.gene005621:CDS	40.0%
	CACAACAAAATACCTTGCAG+GGG	+	8682:78412-78431	MS.gene005621:CDS	40.0%
	CTTTCAATCACTGACCTGTT+TGG	-	8682:78341-78360	None:intergenic	40.0%
	GACTATAAAAATCCCCTGCA+AGG	-	8682:78427-78446	None:intergenic	40.0%
	GCACAACAAAATACCTTGCA+GGG	+	8682:78411-78430	MS.gene005621:CDS	40.0%
	GCATGATTAGTGGTCTAGAT+TGG	-	8682:78035-78054	None:intergenic	40.0%
	GGTGGGAAGATATATCTTCT+AGG	+	8682:78537-78556	MS.gene005621:CDS	40.0%
	GTCTGCTTTCCAAAAAGTTG+TGG	+	8682:78559-78578	MS.gene005621:CDS	40.0%
	TAGATAATTGGTGTGACTGC+AGG	-	8682:78284-78303	None:intergenic	40.0%
	TCAGAAGCAAACACATTGAC+TGG	-	8682:77640-77659	None:intergenic	40.0%
	TGACATGCATGCATGATTAG+TGG	-	8682:78045-78064	None:intergenic	40.0%
!	AACGGATTCATGTTAAGGCT+TGG	+	8682:78516-78535	MS.gene005621:CDS	40.0%
!	GATTCATGTTAAGGCTTGGT+GGG	+	8682:78520-78539	MS.gene005621:CDS	40.0%
!	TCATACCTCCCACAACTTTT+TGG	-	8682:78571-78590	None:intergenic	40.0%
!!	TTTGGTTCTTGGCCTTGAAA+TGG	-	8682:78323-78342	None:intergenic	40.0%
	GATGATGATCTCACCCTCTT+TGG	+	8682:77793-77812	MS.gene005621:CDS	45.0%
	GCTTTCCAAAAAGTTGTGGG+AGG	+	8682:78563-78582	MS.gene005621:CDS	45.0%
	GGATTCATGTTAAGGCTTGG+TGG	+	8682:78519-78538	MS.gene005621:CDS	45.0%
	TAGACATATCATGGCACGAG+TGG	-	8682:77587-77606	None:intergenic	45.0%
	TCTTTGTTCATGCAGCCCTT+TGG	+	8682:77751-77770	MS.gene005621:intron	45.0%
	TTCAAGGCCAAGAACCAAAC+AGG	+	8682:78324-78343	MS.gene005621:CDS	45.0%
!!	TCACTGACCTGTTTGGTTCT+TGG	-	8682:78334-78353	None:intergenic	45.0%
	ACGAGTGGTGTTTGTGGACA+TGG	-	8682:77572-77591	None:intergenic	50.0%
	CGAGTGGTGTTTGTGGACAT+GGG	-	8682:77571-77590	None:intergenic	50.0%
!	GTTGTGGGAGGTATGACCTT+TGG	+	8682:78575-78594	MS.gene005621:CDS	50.0%
	CATGGCACGAGTGGTGTTTG+TGG	-	8682:77578-77597	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
8682	gene	77544	78616	77544	ID=MS.gene005621
8682	mRNA	77544	78616	77544	ID=MS.gene005621.t1;Parent=MS.gene005621
8682	exon	77544	77690	77544	ID=MS.gene005621.t1.exon1;Parent=MS.gene005621.t1
8682	CDS	77544	77690	77544	ID=cds.MS.gene005621.t1;Parent=MS.gene005621.t1
8682	exon	77766	77814	77766	ID=MS.gene005621.t1.exon2;Parent=MS.gene005621.t1
8682	CDS	77766	77814	77766	ID=cds.MS.gene005621.t1;Parent=MS.gene005621.t1
8682	exon	78150	78616	78150	ID=MS.gene005621.t1.exon3;Parent=MS.gene005621.t1
8682	CDS	78150	78616	78150	ID=cds.MS.gene005621.t1;Parent=MS.gene005621.t1

Gene Sequence

>MS.gene005621

ATGGCACTCAAGATTTATGGTCTGCCCATGTCCACAAACACCACTCGTGCCATGATATGTCTACATGAAAAAGAAGTAGATTTTGAGCTTATTCCAGTCAATGTGTTTGCTTCTGAGCATAAACAGCCTCCTTTTCTCAACAAGAACGTAAGTAAAAAAAGCACTTCTAGCTAGCTTATATATCTTTTCTTAATCACATACTAATTTTCTTTGTTCATGCAGCCCTTTGGTTTGATTCCAGTACTTGAAGATGATGATCTCACCCTCTTTGGTATGCTTTCTTTTTTTCTTTTTCAGTTGTTACATTTTATTCACTCTGCACCACCCTTTAAGTTTATTCGAAAAATATACTTTGATGCTCGCTTTCATATTTATAATCAAGCAAAAATACTACTAGGAAAAAAAAAATTATGTAATTTCAGCGCGAGAAGTGACTCTGAAATTTGAGAATTTTTGTCCTCTAATTTTTGTTACTAATTCAACATTTTCCAATCTAGACCACTAATCATGCATGCATGTCAGATAATTTGATATTGTTTTGTTCCATTTTTTGAACCTGTAATGATATGTAACCTATTTTATGTGACTATGGACTATACAAAAAAGAGTCAAGAGCCATAACCTCATATGTAGCTGAAAAATACAAGGAAAATGGACCTGATTTGATTAGGCACAATGACACAAAAGAAGCTTCATTGGTCAAAATGTGGACAGAGGTAGAGTCTCACTACTATGACCCTGCAGTCACACCAATTATCTATGAATACTTTGTGGTTCCATTTCAAGGCCAAGAACCAAACAGGTCAGTGATTGAAAGTAACATTGAGAAGCTGAAGATTGTGCTTGATGTGTATGAGACCAAGCTAAGCACAACAAAATACCTTGCAGGGGATTTTTATAGTCTTGCTGATCTTAGCCACATATCTTCAACTCACTATTTTATGCAGACCCCTTGTGCTTCAATGATCAATGAACGGATTCATGTTAAGGCTTGGTGGGAAGATATATCTTCTAGGTCTGCTTTCCAAAAAGTTGTGGGAGGTATGACCTTTGGTCAGAGTGATCAAAAATGA

Protein sequence

>MS.gene005621.t1

MALKIYGLPMSTNTTRAMICLHEKEVDFELIPVNVFASEHKQPPFLNKNPFGLIPVLEDDDLTLFESRAITSYVAEKYKENGPDLIRHNDTKEASLVKMWTEVESHYYDPAVTPIIYEYFVVPFQGQEPNRSVIESNIEKLKIVLDVYETKLSTTKYLAGDFYSLADLSHISSTHYFMQTPCASMINERIHVKAWWEDISSRSAFQKVVGGMTFGQSDQK