Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005620.t1 | BAB70616.1 | 99.5 | 214 | 1 | 0 | 1 | 214 | 1 | 214 | 7.10E-117 | 429.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005620.t1 | P30109 | 63.6 | 206 | 75 | 0 | 4 | 209 | 3 | 208 | 4.1e-73 | 275.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005620.t1 | Q948X4 | 99.5 | 214 | 1 | 0 | 1 | 214 | 1 | 214 | 5.1e-117 | 429.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene005620 | MS.gene20713 | PPI |
| MS.gene03034 | MS.gene005620 | PPI |
| MS.gene005620 | MS.gene03034 | PPI |
| MS.gene005621 | MS.gene005620 | PPI |
| MS.gene005620 | MS.gene07192 | PPI |
| MS.gene005620 | MS.gene005619 | PPI |
| MS.gene005620 | MS.gene20712 | PPI |
| MS.gene20711 | MS.gene005620 | PPI |
| MS.gene07195 | MS.gene005620 | PPI |
| MS.gene07174 | MS.gene005620 | PPI |
| MS.gene07196 | MS.gene005620 | PPI |
| MS.gene03035 | MS.gene005620 | PPI |
| MS.gene005620 | MS.gene03036 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005620.t1 | MTR_1g088845 | 94.393 | 214 | 12 | 0 | 1 | 214 | 1 | 214 | 1.99e-151 | 419 |
| MS.gene005620.t1 | MTR_1g088840 | 89.252 | 214 | 23 | 0 | 1 | 214 | 1 | 214 | 5.09e-144 | 400 |
| MS.gene005620.t1 | MTR_1g088825 | 89.252 | 214 | 23 | 0 | 1 | 214 | 1 | 214 | 5.09e-144 | 400 |
| MS.gene005620.t1 | MTR_1g088840 | 88.554 | 166 | 19 | 0 | 49 | 214 | 2 | 167 | 4.81e-108 | 307 |
| MS.gene005620.t1 | MTR_1g088840 | 87.288 | 118 | 15 | 0 | 97 | 214 | 1 | 118 | 4.77e-72 | 214 |
| MS.gene005620.t1 | MTR_3g064700 | 43.478 | 207 | 116 | 1 | 3 | 208 | 2 | 208 | 4.21e-57 | 180 |
| MS.gene005620.t1 | MTR_3g450930 | 42.857 | 210 | 118 | 2 | 3 | 211 | 2 | 210 | 9.91e-57 | 179 |
| MS.gene005620.t1 | MTR_1g088850 | 39.423 | 208 | 125 | 1 | 4 | 210 | 3 | 210 | 3.08e-55 | 175 |
| MS.gene005620.t1 | MTR_3g450790 | 41.429 | 210 | 121 | 2 | 3 | 211 | 2 | 210 | 4.86e-55 | 175 |
| MS.gene005620.t1 | MTR_1g026140 | 40.909 | 220 | 123 | 4 | 1 | 213 | 1 | 220 | 3.27e-54 | 173 |
| MS.gene005620.t1 | MTR_5g090920 | 41.429 | 210 | 118 | 4 | 3 | 209 | 2 | 209 | 2.12e-51 | 166 |
| MS.gene005620.t1 | MTR_5g090910 | 38.863 | 211 | 126 | 3 | 3 | 211 | 2 | 211 | 2.57e-49 | 160 |
| MS.gene005620.t1 | MTR_1g492670 | 89.706 | 68 | 7 | 0 | 147 | 214 | 2 | 69 | 4.30e-40 | 132 |
| MS.gene005620.t1 | MTR_5g090910 | 37.415 | 147 | 90 | 2 | 67 | 211 | 8 | 154 | 2.70e-28 | 104 |
| MS.gene005620.t1 | MTR_8g098420 | 27.211 | 147 | 107 | 0 | 4 | 150 | 3 | 149 | 2.70e-11 | 60.5 |
| MS.gene005620.t1 | MTR_8g098420 | 27.397 | 146 | 106 | 0 | 4 | 149 | 3 | 148 | 7.10e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene005620.t1 | AT1G02920 | 59.809 | 209 | 84 | 0 | 1 | 209 | 1 | 209 | 3.77e-86 | 253 |
| MS.gene005620.t1 | AT1G02930 | 58.852 | 209 | 85 | 1 | 1 | 209 | 1 | 208 | 1.01e-85 | 253 |
| MS.gene005620.t1 | AT1G02930 | 58.852 | 209 | 85 | 1 | 1 | 209 | 1 | 208 | 1.01e-85 | 253 |
| MS.gene005620.t1 | AT2G02930 | 58.491 | 212 | 85 | 1 | 1 | 209 | 1 | 212 | 4.23e-85 | 251 |
| MS.gene005620.t1 | AT4G02520 | 57.346 | 211 | 87 | 1 | 1 | 208 | 1 | 211 | 1.61e-84 | 249 |
| MS.gene005620.t1 | AT2G47730 | 55.610 | 205 | 90 | 1 | 6 | 209 | 54 | 258 | 1.93e-83 | 249 |
| MS.gene005620.t1 | AT2G47730 | 55.610 | 205 | 90 | 1 | 6 | 209 | 54 | 258 | 1.93e-83 | 249 |
| MS.gene005620.t1 | AT1G02950 | 51.942 | 206 | 98 | 1 | 1 | 205 | 24 | 229 | 4.60e-77 | 232 |
| MS.gene005620.t1 | AT1G02950 | 52.475 | 202 | 95 | 1 | 5 | 205 | 26 | 227 | 1.27e-76 | 231 |
| MS.gene005620.t1 | AT1G02950 | 52.475 | 202 | 95 | 1 | 5 | 205 | 26 | 227 | 1.27e-76 | 231 |
| MS.gene005620.t1 | AT1G02950 | 52.475 | 202 | 95 | 1 | 5 | 205 | 38 | 239 | 1.62e-76 | 231 |
| MS.gene005620.t1 | AT1G02940 | 46.602 | 206 | 109 | 1 | 5 | 209 | 40 | 245 | 4.17e-67 | 207 |
| MS.gene005620.t1 | AT1G02940 | 46.602 | 206 | 109 | 1 | 5 | 209 | 40 | 245 | 4.17e-67 | 207 |
| MS.gene005620.t1 | AT1G02940 | 46.602 | 206 | 109 | 1 | 5 | 209 | 53 | 258 | 6.27e-67 | 207 |
| MS.gene005620.t1 | AT1G02940 | 46.602 | 206 | 109 | 1 | 5 | 209 | 65 | 270 | 8.25e-67 | 207 |
| MS.gene005620.t1 | AT3G62760 | 45.631 | 206 | 111 | 1 | 4 | 208 | 3 | 208 | 5.39e-61 | 190 |
| MS.gene005620.t1 | AT1G49860 | 47.692 | 195 | 98 | 2 | 21 | 211 | 22 | 216 | 6.70e-57 | 181 |
| MS.gene005620.t1 | AT3G03190 | 42.381 | 210 | 120 | 1 | 3 | 211 | 2 | 211 | 7.00e-54 | 172 |
| MS.gene005620.t1 | AT5G17220 | 38.095 | 210 | 129 | 1 | 3 | 211 | 2 | 211 | 2.36e-50 | 163 |
| MS.gene005620.t1 | AT2G30860 | 37.321 | 209 | 129 | 2 | 3 | 210 | 2 | 209 | 1.18e-46 | 153 |
| MS.gene005620.t1 | AT2G30870 | 36.946 | 203 | 127 | 1 | 9 | 210 | 7 | 209 | 3.14e-45 | 150 |
| MS.gene005620.t1 | AT1G02950 | 47.887 | 142 | 73 | 1 | 65 | 205 | 60 | 201 | 7.17e-44 | 146 |
| MS.gene005620.t1 | AT2G30860 | 35.294 | 153 | 98 | 1 | 3 | 155 | 2 | 153 | 7.21e-29 | 106 |
Find 0 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGTCTGAATCTAAATACA+TGG | - | 8682:65585-65604 | MS.gene005620:intron | 25.0% |
| ! | TTGTCTGAATCTAAATACAT+GGG | - | 8682:65586-65605 | MS.gene005620:intron | 25.0% |
| !! | ATCAATTAGTTTGATCAATG+TGG | + | 8682:65066-65085 | None:intergenic | 25.0% |
| !! | CTTACATTGATTGATATGAA+GGG | + | 8682:64974-64993 | None:intergenic | 25.0% |
| AAAATTGAAATGCAGAGTCA+AGG | - | 8682:65317-65336 | MS.gene005620:intron | 30.0% | |
| AAATTGAAATGCAGAGTCAA+GGG | - | 8682:65318-65337 | MS.gene005620:intron | 30.0% | |
| AAGACCTTATGTTAATGCTT+GGG | - | 8682:65696-65715 | MS.gene005620:CDS | 30.0% | |
| AAGTAGCATCAATACTTGTT+TGG | - | 8682:65461-65480 | MS.gene005620:intron | 30.0% | |
| AGTAGCATCAATACTTGTTT+GGG | - | 8682:65462-65481 | MS.gene005620:intron | 30.0% | |
| AGTTTGTAAGCATTAACATG+AGG | - | 8682:64927-64946 | MS.gene005620:CDS | 30.0% | |
| CTAGGGATTAAGACTTTATT+TGG | - | 8682:65487-65506 | MS.gene005620:CDS | 30.0% | |
| ! | ATTAGTTTGATCAATGTGGT+TGG | + | 8682:65062-65081 | None:intergenic | 30.0% |
| ! | GACTTTATTTGGAATTCCTT+TGG | - | 8682:65498-65517 | MS.gene005620:intron | 30.0% |
| ! | GCTTACATTGATTGATATGA+AGG | + | 8682:64975-64994 | None:intergenic | 30.0% |
| AAATCTCCATCTTCAAAAGC+TGG | + | 8682:65122-65141 | None:intergenic | 35.0% | |
| ACTCATTATTGCCATCTTCT+TGG | + | 8682:65407-65426 | None:intergenic | 35.0% | |
| ATCAAGAATGCTATCCAACT+TGG | + | 8682:65551-65570 | None:intergenic | 35.0% | |
| ATCATGAGTATGCTGATAAG+GGG | - | 8682:65359-65378 | MS.gene005620:intron | 35.0% | |
| CAAGACCTTATGTTAATGCT+TGG | - | 8682:65695-65714 | MS.gene005620:CDS | 35.0% | |
| CAATACTTGTTTGGGAACTA+GGG | - | 8682:65470-65489 | MS.gene005620:CDS | 35.0% | |
| GATCATGAGTATGCTGATAA+GGG | - | 8682:65358-65377 | MS.gene005620:intron | 35.0% | |
| GTAAGCATTAACATGAGGAA+TGG | - | 8682:64932-64951 | MS.gene005620:CDS | 35.0% | |
| TAAATGATGCAAATCCACCA+AGG | + | 8682:65626-65645 | None:intergenic | 35.0% | |
| TCAACCCAAGCATTAACATA+AGG | + | 8682:65703-65722 | None:intergenic | 35.0% | |
| TCAATACTTGTTTGGGAACT+AGG | - | 8682:65469-65488 | MS.gene005620:CDS | 35.0% | |
| TCCTTTGGATTCAAAAGTTG+TGG | - | 8682:65513-65532 | MS.gene005620:intron | 35.0% | |
| TCTGAATCTAAATACATGGG+AGG | - | 8682:65589-65608 | MS.gene005620:intron | 35.0% | |
| TGATCATGAGTATGCTGATA+AGG | - | 8682:65357-65376 | MS.gene005620:intron | 35.0% | |
| TTCATCAAGTAGTGAAGAGA+AGG | + | 8682:65649-65668 | None:intergenic | 35.0% | |
| TTCTTTATGTTGCAGCCATT+TGG | - | 8682:65089-65108 | MS.gene005620:CDS | 35.0% | |
| ! | ATGGCAACAATAAAAGTGCA+CGG | - | 8682:64839-64858 | MS.gene005620:CDS | 35.0% |
| ! | GATGGAGATTTAAAGCTCTT+TGG | - | 8682:65131-65150 | MS.gene005620:CDS | 35.0% |
| ! | TCCACAACTTTTGAATCCAA+AGG | + | 8682:65517-65536 | None:intergenic | 35.0% |
| !! | ATGCTACTTTATCGTAGTGT+AGG | + | 8682:65450-65469 | None:intergenic | 35.0% |
| AAAGCTGGAACTTGACCAAA+TGG | + | 8682:65107-65126 | None:intergenic | 40.0% | |
| AGATGGCAATAATGAGTGTG+TGG | - | 8682:65410-65429 | MS.gene005620:intron | 40.0% | |
| GAGGAATGGTGAACACAAAA+AGG | - | 8682:64946-64965 | MS.gene005620:CDS | 40.0% | |
| TCAATGTGGTTGGAACAAGA+AGG | + | 8682:65052-65071 | None:intergenic | 40.0% | |
| !! | CAAGTTCCAGCTTTTGAAGA+TGG | - | 8682:65113-65132 | MS.gene005620:CDS | 40.0% |
| AAGCAGTGACTCCAAGAAGA+TGG | - | 8682:65393-65412 | MS.gene005620:intron | 45.0% | |
| ATATCACTGCAAGACCAGCT+TGG | - | 8682:65725-65744 | MS.gene005620:CDS | 45.0% | |
| GACACGATATCAAACACACG+TGG | - | 8682:65215-65234 | MS.gene005620:CDS | 45.0% | |
| GCAAGTACTTTAGACCAAGC+TGG | + | 8682:65742-65761 | None:intergenic | 45.0% | |
| GGAAGAGAATGAAGCCAAGT+TGG | - | 8682:65534-65553 | MS.gene005620:intron | 45.0% | |
| GGCAATAATGAGTGTGTGGT+CGG | - | 8682:65414-65433 | MS.gene005620:intron | 45.0% | |
| AATGAGTGTGTGGTCGGAAG+TGG | - | 8682:65420-65439 | MS.gene005620:intron | 50.0% | |
| AGGTGAGTGTTTCACCTTGG+TGG | - | 8682:65609-65628 | MS.gene005620:intron | 50.0% | |
| AGTTGCAGTAACCCGCATGG+TGG | + | 8682:64882-64901 | None:intergenic | 55.0% | |
| CAGAGTTGCAGTAACCCGCA+TGG | + | 8682:64885-64904 | None:intergenic | 55.0% | |
| CTATCTCAACGGCCACCATG+CGG | - | 8682:64867-64886 | MS.gene005620:CDS | 55.0% | |
| GCACGGAAGCCCTATCTCAA+CGG | - | 8682:64856-64875 | MS.gene005620:CDS | 55.0% | |
| GCATGGTGGCCGTTGAGATA+GGG | + | 8682:64868-64887 | None:intergenic | 55.0% | |
| GGGAGGTGAGTGTTTCACCT+TGG | - | 8682:65606-65625 | MS.gene005620:intron | 55.0% | |
| TATCTCAACGGCCACCATGC+GGG | - | 8682:64868-64887 | MS.gene005620:CDS | 55.0% | |
| CGCATGGTGGCCGTTGAGAT+AGG | + | 8682:64869-64888 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8682 | gene | 64839 | 65777 | 64839 | ID=MS.gene005620 |
| 8682 | mRNA | 64839 | 65777 | 64839 | ID=MS.gene005620.t1;Parent=MS.gene005620 |
| 8682 | exon | 65628 | 65777 | 65628 | ID=MS.gene005620.t1.exon1;Parent=MS.gene005620.t1 |
| 8682 | CDS | 65628 | 65777 | 65628 | ID=cds.MS.gene005620.t1;Parent=MS.gene005620.t1 |
| 8682 | exon | 65464 | 65512 | 65464 | ID=MS.gene005620.t1.exon2;Parent=MS.gene005620.t1 |
| 8682 | CDS | 65464 | 65512 | 65464 | ID=cds.MS.gene005620.t1;Parent=MS.gene005620.t1 |
| 8682 | exon | 64839 | 65284 | 64839 | ID=MS.gene005620.t1.exon3;Parent=MS.gene005620.t1 |
| 8682 | CDS | 64839 | 65284 | 64839 | ID=cds.MS.gene005620.t1;Parent=MS.gene005620.t1 |
| Gene Sequence |
| Protein sequence |
