AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07195


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07195.t1 MTR_1g088845 96.262 214 8 0 1 214 1 214 1.96e-153 424
MS.gene07195.t1 MTR_1g088840 90.654 214 20 0 1 214 1 214 9.60e-146 404
MS.gene07195.t1 MTR_1g088825 90.654 214 20 0 1 214 1 214 9.60e-146 404
MS.gene07195.t1 MTR_1g088840 90.361 166 16 0 49 214 2 167 1.05e-109 311
MS.gene07195.t1 MTR_1g088840 89.831 118 12 0 97 214 1 118 7.13e-74 219
MS.gene07195.t1 MTR_3g064700 44.928 207 113 1 3 208 2 208 3.22e-59 185
MS.gene07195.t1 MTR_3g450930 43.810 210 116 2 3 211 2 210 5.80e-58 182
MS.gene07195.t1 MTR_1g088850 40.865 208 122 1 4 210 3 210 9.19e-57 179
MS.gene07195.t1 MTR_1g026140 42.273 220 120 4 1 213 1 220 2.60e-56 178
MS.gene07195.t1 MTR_3g450790 42.381 210 119 2 3 211 2 210 4.96e-56 177
MS.gene07195.t1 MTR_5g090920 42.381 210 116 4 3 209 2 209 1.41e-52 168
MS.gene07195.t1 MTR_5g090910 39.810 211 124 3 3 211 2 211 1.13e-50 164
MS.gene07195.t1 MTR_1g492670 92.647 68 5 0 147 214 2 69 5.05e-41 134
MS.gene07195.t1 MTR_5g090910 38.776 147 88 2 67 211 8 154 1.35e-29 108
MS.gene07195.t1 MTR_8g098420 27.211 147 107 0 4 150 3 149 2.78e-11 60.5
MS.gene07195.t1 MTR_8g098420 27.397 146 106 0 4 149 3 148 6.44e-11 60.5
MS.gene07195.t1 MTR_4g134380 25.000 168 115 3 12 175 24 184 6.47e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07195.t1 AT1G02920 61.244 209 81 0 1 209 1 209 9.66e-88 258
MS.gene07195.t1 AT1G02930 60.287 209 82 1 1 209 1 208 3.43e-87 256
MS.gene07195.t1 AT1G02930 60.287 209 82 1 1 209 1 208 3.43e-87 256
MS.gene07195.t1 AT2G47730 57.561 205 86 1 6 209 54 258 9.66e-86 254
MS.gene07195.t1 AT2G47730 57.561 205 86 1 6 209 54 258 9.66e-86 254
MS.gene07195.t1 AT2G02930 58.962 212 84 1 1 209 1 212 1.56e-85 252
MS.gene07195.t1 AT4G02520 57.820 211 86 1 1 208 1 211 5.62e-85 251
MS.gene07195.t1 AT1G02950 52.913 206 96 1 1 205 24 229 1.07e-78 236
MS.gene07195.t1 AT1G02950 53.465 202 93 1 5 205 26 227 2.08e-78 235
MS.gene07195.t1 AT1G02950 53.465 202 93 1 5 205 26 227 2.08e-78 235
MS.gene07195.t1 AT1G02950 53.465 202 93 1 5 205 38 239 2.65e-78 235
MS.gene07195.t1 AT1G02940 47.573 206 107 1 5 209 40 245 4.75e-69 212
MS.gene07195.t1 AT1G02940 47.573 206 107 1 5 209 40 245 4.75e-69 212
MS.gene07195.t1 AT1G02940 47.573 206 107 1 5 209 65 270 1.13e-68 212
MS.gene07195.t1 AT1G02940 47.573 206 107 1 5 209 53 258 1.21e-68 211
MS.gene07195.t1 AT3G62760 47.087 206 108 1 4 208 3 208 4.68e-63 195
MS.gene07195.t1 AT1G49860 48.205 195 97 2 21 211 22 216 1.06e-57 183
MS.gene07195.t1 AT3G03190 43.810 210 117 1 3 211 2 211 5.93e-56 177
MS.gene07195.t1 AT5G17220 38.095 210 129 1 3 211 2 211 5.51e-51 164
MS.gene07195.t1 AT2G30860 38.756 209 126 2 3 210 2 209 7.94e-48 156
MS.gene07195.t1 AT2G30870 38.424 203 124 1 9 210 7 209 2.14e-46 152
MS.gene07195.t1 AT1G02950 49.296 142 71 1 65 205 60 201 9.46e-46 151
MS.gene07195.t1 AT2G30860 35.948 153 97 1 3 155 2 153 2.63e-29 107
MS.gene07195.t1 AT1G09640 29.825 171 113 5 41 210 40 204 2.75e-11 62.4
MS.gene07195.t1 AT1G09640 29.586 169 112 5 41 208 40 202 3.78e-11 61.2
MS.gene07195.t1 AT2G02380 29.189 185 105 6 2 175 10 179 6.24e-11 60.1

Find 49 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGGGATTAAGACTTTATT+TGG 0.143067 1.4:-70498593 MS.gene07195:CDS
GATGGAGATTTAAAGCTCTT+TGG 0.296201 1.4:-70498954 MS.gene07195:intron
GACTTTATTTGGAATTCCTT+TGG 0.310575 1.4:-70498582 MS.gene07195:CDS
CGCATGGTGGCCGTTGAGAT+AGG 0.336998 1.4:+70499215 None:intergenic
TCAATACTTGTTTGGGAACT+AGG 0.337085 1.4:-70498611 MS.gene07195:CDS
AAGTAGCATCAATACTTGTT+TGG 0.355376 1.4:-70498619 MS.gene07195:CDS
GAGGAATGGTGAACACAAAA+AGG 0.363061 1.4:-70499135 MS.gene07195:CDS
AGTAGCATCAATACTTGTTT+GGG 0.373002 1.4:-70498618 MS.gene07195:CDS
ATCTTTATGTTGCAGCCATT+TGG 0.379956 1.4:-70498996 MS.gene07195:intron
GATCATGAGTATGCTGATAA+GGG 0.387666 1.4:-70498722 MS.gene07195:CDS
TGATCATGAGTATGCTGATA+AGG 0.403067 1.4:-70498723 MS.gene07195:CDS
CAAGACCTTATGTTAATGCT+TGG 0.407435 1.4:-70498385 MS.gene07195:CDS
AAGACCTTATGTTAATGCTT+GGG 0.420971 1.4:-70498384 MS.gene07195:CDS
AGGTGACTCTTTCACCTTGG+TGG 0.445525 1.4:-70498471 MS.gene07195:CDS
AAATCTCCATCTTCAAAAGC+TGG 0.446504 1.4:+70498966 None:intergenic
ATGATCTTAATTAGGTATCA+TGG 0.448138 1.4:+70498319 None:intergenic
ATTGTCTGAATCTAAATACT+TGG 0.454334 1.4:-70498495 MS.gene07195:CDS
GCTTACATTGATTGATATGA+AGG 0.465911 1.4:+70499109 None:intergenic
ATGCTACTTTATCGTAGTGT+AGG 0.467657 1.4:+70498633 None:intergenic
CAATACTTGTTTGGGAACTA+GGG 0.472992 1.4:-70498610 MS.gene07195:CDS
AAAGCTGGAACTTGACCAAA+TGG 0.480424 1.4:+70498981 None:intergenic
TCCTTTGGATTCAAAAGTTG+TGG 0.481662 1.4:-70498567 MS.gene07195:CDS
AATGAGTGTGTGGTCGGAAG+TGG 0.508607 1.4:-70498660 MS.gene07195:CDS
GCATGGTGGCCGTTGAGATA+GGG 0.515923 1.4:+70499216 None:intergenic
GGGAGGTGACTCTTTCACCT+TGG 0.520363 1.4:-70498474 MS.gene07195:CDS
TCAACCCAAGCATTAACATA+AGG 0.524477 1.4:+70498380 None:intergenic
ATCAAGAATGCTATCCAACT+TGG 0.528639 1.4:+70498532 None:intergenic
TATCTCAACGGCCACCATGC+GGG 0.531171 1.4:-70499213 MS.gene07195:CDS
GGAAGAGAATGAAGCCAAGT+TGG 0.538389 1.4:-70498546 MS.gene07195:CDS
AAAATTGAAATGCAGAGTCA+AGG 0.547213 1.4:-70498763 MS.gene07195:intron
AAATTGAAATGCAGAGTCAA+GGG 0.557402 1.4:-70498762 MS.gene07195:intron
TTCATCAAGTAGTGAAGAGA+AGG 0.557567 1.4:+70498434 None:intergenic
GTAAGCATTAACATGAGGAA+TGG 0.557734 1.4:-70499149 MS.gene07195:CDS
AAGCAGTGACTCGAAGAAGA+TGG 0.567141 1.4:-70498687 MS.gene07195:CDS
GCAAGTACTTTAGACCAAGC+TGG 0.578209 1.4:+70498341 None:intergenic
CTTACATTGATTGATATGAA+GGG 0.578372 1.4:+70499110 None:intergenic
GGCAATAATGAGTGTGTGGT+CGG 0.583453 1.4:-70498666 MS.gene07195:CDS
TTGTCTGAATCTAAATACTT+GGG 0.593351 1.4:-70498494 MS.gene07195:CDS
ATATCACTGCAAGACCAGCT+TGG 0.601324 1.4:-70498355 MS.gene07195:CDS
ATCATGAGTATGCTGATAAG+GGG 0.611479 1.4:-70498721 MS.gene07195:CDS
GCACGGAAGCCCTATCTCAA+CGG 0.620985 1.4:-70499225 MS.gene07195:CDS
CTATCTCAACGGCCACCATG+CGG 0.624610 1.4:-70499214 MS.gene07195:CDS
AGTTTGTAAGCATTAACATG+AGG 0.629431 1.4:-70499154 MS.gene07195:CDS
ATGGCAACAATAAAAGTGCA+CGG 0.649349 1.4:-70499242 MS.gene07195:CDS
CAGAGTTGCAGTAACCCGCA+TGG 0.653094 1.4:+70499199 None:intergenic
TCTGAATCTAAATACTTGGG+AGG 0.673210 1.4:-70498491 MS.gene07195:CDS
AGTTGCAGTAACCCGCATGG+TGG 0.707787 1.4:+70499202 None:intergenic
TAAATGATGCAAATCCACCA+AGG 0.710594 1.4:+70498457 None:intergenic
AGATGGCAATAATGAGTGTG+TGG 0.714038 1.4:-70498670 MS.gene07195:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATAAAGATTGAATTAGT+TGG + chr1.4:70498562-70498581 None:intergenic 20.0%
! ATTGTCTGAATCTAAATACT+TGG - chr1.4:70499072-70499091 MS.gene07195:intron 25.0%
! TTGTCTGAATCTAAATACTT+GGG - chr1.4:70499073-70499092 MS.gene07195:intron 25.0%
!! CTTACATTGATTGATATGAA+GGG + chr1.4:70498460-70498479 None:intergenic 25.0%
AAAATTGAAATGCAGAGTCA+AGG - chr1.4:70498804-70498823 MS.gene07195:intron 30.0%
AAATTGAAATGCAGAGTCAA+GGG - chr1.4:70498805-70498824 MS.gene07195:intron 30.0%
AAGACCTTATGTTAATGCTT+GGG - chr1.4:70499183-70499202 MS.gene07195:CDS 30.0%
AAGTAGCATCAATACTTGTT+TGG - chr1.4:70498948-70498967 MS.gene07195:intron 30.0%
AGTAGCATCAATACTTGTTT+GGG - chr1.4:70498949-70498968 MS.gene07195:intron 30.0%
AGTTTGTAAGCATTAACATG+AGG - chr1.4:70498413-70498432 MS.gene07195:CDS 30.0%
ATTAGTTGGATCAATGTAGT+TGG + chr1.4:70498548-70498567 None:intergenic 30.0%
CTAGGGATTAAGACTTTATT+TGG - chr1.4:70498974-70498993 MS.gene07195:CDS 30.0%
! GACTTTATTTGGAATTCCTT+TGG - chr1.4:70498985-70499004 MS.gene07195:intron 30.0%
! GCTTACATTGATTGATATGA+AGG + chr1.4:70498461-70498480 None:intergenic 30.0%
AAATCTCCATCTTCAAAAGC+TGG + chr1.4:70498604-70498623 None:intergenic 35.0%
ATCAAGAATGCTATCCAACT+TGG + chr1.4:70499038-70499057 None:intergenic 35.0%
ATCATGAGTATGCTGATAAG+GGG - chr1.4:70498846-70498865 MS.gene07195:intron 35.0%
ATCTTTATGTTGCAGCCATT+TGG - chr1.4:70498571-70498590 MS.gene07195:CDS 35.0%
CAAGACCTTATGTTAATGCT+TGG - chr1.4:70499182-70499201 MS.gene07195:CDS 35.0%
CAATACTTGTTTGGGAACTA+GGG - chr1.4:70498957-70498976 MS.gene07195:CDS 35.0%
GATCATGAGTATGCTGATAA+GGG - chr1.4:70498845-70498864 MS.gene07195:intron 35.0%
GTAAGCATTAACATGAGGAA+TGG - chr1.4:70498418-70498437 MS.gene07195:CDS 35.0%
TAAATGATGCAAATCCACCA+AGG + chr1.4:70499113-70499132 None:intergenic 35.0%
TCAACCCAAGCATTAACATA+AGG + chr1.4:70499190-70499209 None:intergenic 35.0%
TCAATACTTGTTTGGGAACT+AGG - chr1.4:70498956-70498975 MS.gene07195:CDS 35.0%
TCAATGTAGTTGGAACAAGA+AGG + chr1.4:70498538-70498557 None:intergenic 35.0%
TCCTTTGGATTCAAAAGTTG+TGG - chr1.4:70499000-70499019 MS.gene07195:intron 35.0%
TCTGAATCTAAATACTTGGG+AGG - chr1.4:70499076-70499095 MS.gene07195:intron 35.0%
TGATCATGAGTATGCTGATA+AGG - chr1.4:70498844-70498863 MS.gene07195:intron 35.0%
TTCATCAAGTAGTGAAGAGA+AGG + chr1.4:70499136-70499155 None:intergenic 35.0%
! ATGGCAACAATAAAAGTGCA+CGG - chr1.4:70498325-70498344 MS.gene07195:CDS 35.0%
! GATGGAGATTTAAAGCTCTT+TGG - chr1.4:70498613-70498632 MS.gene07195:CDS 35.0%
! TCCACAACTTTTGAATCCAA+AGG + chr1.4:70499004-70499023 None:intergenic 35.0%
!! ATGCTACTTTATCGTAGTGT+AGG + chr1.4:70498937-70498956 None:intergenic 35.0%
AAAGCTGGAACTTGACCAAA+TGG + chr1.4:70498589-70498608 None:intergenic 40.0%
AGATGGCAATAATGAGTGTG+TGG - chr1.4:70498897-70498916 MS.gene07195:intron 40.0%
GAGGAATGGTGAACACAAAA+AGG - chr1.4:70498432-70498451 MS.gene07195:CDS 40.0%
!! CAAGTTCCAGCTTTTGAAGA+TGG - chr1.4:70498595-70498614 MS.gene07195:CDS 40.0%
AAGCAGTGACTCGAAGAAGA+TGG - chr1.4:70498880-70498899 MS.gene07195:intron 45.0%
ATATCACTGCAAGACCAGCT+TGG - chr1.4:70499212-70499231 MS.gene07195:CDS 45.0%
GACACGATATCAAACACACG+TGG - chr1.4:70498702-70498721 MS.gene07195:CDS 45.0%
GCAAGTACTTTAGACCAAGC+TGG + chr1.4:70499229-70499248 None:intergenic 45.0%
GGAAGAGAATGAAGCCAAGT+TGG - chr1.4:70499021-70499040 MS.gene07195:intron 45.0%
GGCAATAATGAGTGTGTGGT+CGG - chr1.4:70498901-70498920 MS.gene07195:intron 45.0%
AATGAGTGTGTGGTCGGAAG+TGG - chr1.4:70498907-70498926 MS.gene07195:intron 50.0%
AGGTGACTCTTTCACCTTGG+TGG - chr1.4:70499096-70499115 MS.gene07195:intron 50.0%
AGTTGCAGTAACCCGCATGG+TGG + chr1.4:70498368-70498387 None:intergenic 55.0%
CAGAGTTGCAGTAACCCGCA+TGG + chr1.4:70498371-70498390 None:intergenic 55.0%
CTATCTCAACGGCCACCATG+CGG - chr1.4:70498353-70498372 MS.gene07195:CDS 55.0%
GCACGGAAGCCCTATCTCAA+CGG - chr1.4:70498342-70498361 MS.gene07195:CDS 55.0%
GCATGGTGGCCGTTGAGATA+GGG + chr1.4:70498354-70498373 None:intergenic 55.0%
GGGAGGTGACTCTTTCACCT+TGG - chr1.4:70499093-70499112 MS.gene07195:intron 55.0%
TATCTCAACGGCCACCATGC+GGG - chr1.4:70498354-70498373 MS.gene07195:CDS 55.0%
CGCATGGTGGCCGTTGAGAT+AGG + chr1.4:70498355-70498374 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 70498325 70499264 70498325 ID=MS.gene07195
chr1.4 mRNA 70498325 70499264 70498325 ID=MS.gene07195.t1;Parent=MS.gene07195
chr1.4 exon 70499115 70499264 70499115 ID=MS.gene07195.t1.exon1;Parent=MS.gene07195.t1
chr1.4 CDS 70499115 70499264 70499115 ID=cds.MS.gene07195.t1;Parent=MS.gene07195.t1
chr1.4 exon 70498955 70499003 70498955 ID=MS.gene07195.t1.exon2;Parent=MS.gene07195.t1
chr1.4 CDS 70498955 70499003 70498955 ID=cds.MS.gene07195.t1;Parent=MS.gene07195.t1
chr1.4 exon 70498325 70498770 70498325 ID=MS.gene07195.t1.exon3;Parent=MS.gene07195.t1
chr1.4 CDS 70498325 70498770 70498325 ID=cds.MS.gene07195.t1;Parent=MS.gene07195.t1
Gene Sequence

>MS.gene07195

ATGGCAACAATAAAAGTGCACGGAAGCCCTATCTCAACGGCCACCATGCGGGTTACTGCAACTCTGTATGAGAAAGAGCTTGAATTTGAGTTTGTAAGCATTAACATGAGGAATGGTGAACACAAAAAGGAACCCTTCATATCAATCAATGTAAGCAACATAACAAAAATATCATTCTGCTAGTTTACTTTAATTATGAATTGAAATTTTCCTTCTTGTTCCAACTACATTGATCCAACTAATTCAATCTTTATGTTGCAGCCATTTGGTCAAGTTCCAGCTTTTGAAGATGGAGATTTAAAGCTCTTTGGTAAAATTTATTTGAATATGATATATGTAGCACATGACACTTTAGATTAAAAACGTATTTGATGTCTGACACGATATCAAACACACGTGGTTATATTCAATTATTTTTATTTGATGTCTAGTATTTGTGCGAGTGCTTCATAGAATTTGTTGTATATATAAACTAATTAAAAATTGAAATGCAGAGTCAAGGGCGATAACACAATATATTGATCATGAGTATGCTGATAAGGGGACTAAGTTAACAAGCAGTGACTCGAAGAAGATGGCAATAATGAGTGTGTGGTCGGAAGTGGAAAGCCTACACTACGATAAAGTAGCATCAATACTTGTTTGGGAACTAGGGATTAAGACTTTATTTGGAATTCCTTTGGATTCAAAAGTTGTGGAAGAGAATGAAGCCAAGTTGGATAGCATTCTTGATGTGTATGAGAAAAGATTGTCTGAATCTAAATACTTGGGAGGTGACTCTTTCACCTTGGTGGATTTGCATCATTTACCTTCTCTTCACTACTTGATGAAGTCACAGAGCAAGAAATTGTTTGAATCAAGACCTTATGTTAATGCTTGGGTTGATGATATCACTGCAAGACCAGCTTGGTCTAAAGTACTTGCCATGATACCTAATTAA

Protein sequence

>MS.gene07195.t1

MATIKVHGSPISTATMRVTATLYEKELEFEFVSINMRNGEHKKEPFISINPFGQVPAFEDGDLKLFESRAITQYIDHEYADKGTKLTSSDSKKMAIMSVWSEVESLHYDKVASILVWELGIKTLFGIPLDSKVVEENEAKLDSILDVYEKRLSESKYLGGDSFTLVDLHHLPSLHYLMKSQSKKLFESRPYVNAWVDDITARPAWSKVLAMIPN