AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03036


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03036.t1 MTR_1g088840 92.523 214 16 0 1 214 1 214 1.65e-149 414
MS.gene03036.t1 MTR_1g088825 92.523 214 16 0 1 214 1 214 1.65e-149 414
MS.gene03036.t1 MTR_1g088845 86.916 214 28 0 1 214 1 214 1.55e-139 389
MS.gene03036.t1 MTR_1g088840 91.566 166 14 0 49 214 2 167 3.83e-112 318
MS.gene03036.t1 MTR_1g088840 92.373 118 9 0 97 214 1 118 3.62e-77 228
MS.gene03036.t1 MTR_3g064700 46.117 206 110 1 4 208 3 208 3.00e-60 188
MS.gene03036.t1 MTR_3g450930 44.498 209 114 2 4 211 3 210 4.70e-59 185
MS.gene03036.t1 MTR_3g450790 44.019 209 115 2 4 211 3 210 3.63e-58 182
MS.gene03036.t1 MTR_1g088850 39.713 209 125 1 3 210 2 210 1.57e-56 179
MS.gene03036.t1 MTR_5g090920 44.019 209 112 4 4 209 3 209 8.61e-56 177
MS.gene03036.t1 MTR_1g026140 42.130 216 119 3 4 213 5 220 2.46e-55 176
MS.gene03036.t1 MTR_5g090910 41.905 210 119 3 4 211 3 211 1.30e-54 174
MS.gene03036.t1 MTR_1g492670 86.765 68 9 0 147 214 2 69 6.11e-39 129
MS.gene03036.t1 MTR_5g090910 40.136 147 86 2 67 211 8 154 3.59e-32 114
MS.gene03036.t1 MTR_3g088635 23.013 239 152 5 4 210 1 239 2.96e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03036.t1 AT1G02920 63.158 209 77 0 1 209 1 209 5.04e-89 261
MS.gene03036.t1 AT1G02930 61.722 209 79 1 1 209 1 208 6.37e-88 258
MS.gene03036.t1 AT1G02930 61.722 209 79 1 1 209 1 208 6.37e-88 258
MS.gene03036.t1 AT2G47730 58.049 205 85 1 6 209 54 258 3.82e-86 256
MS.gene03036.t1 AT2G47730 58.049 205 85 1 6 209 54 258 3.82e-86 256
MS.gene03036.t1 AT4G02520 59.242 211 83 1 1 208 1 211 6.27e-85 251
MS.gene03036.t1 AT2G02930 58.962 212 84 1 1 209 1 212 3.66e-83 246
MS.gene03036.t1 AT1G02950 54.369 206 93 1 1 205 24 229 5.48e-78 234
MS.gene03036.t1 AT1G02950 54.950 202 90 1 5 205 26 227 2.61e-77 232
MS.gene03036.t1 AT1G02950 54.950 202 90 1 5 205 26 227 2.61e-77 232
MS.gene03036.t1 AT1G02950 54.950 202 90 1 5 205 38 239 2.86e-77 233
MS.gene03036.t1 AT1G02940 47.087 206 108 1 5 209 40 245 2.56e-66 205
MS.gene03036.t1 AT1G02940 47.087 206 108 1 5 209 40 245 2.56e-66 205
MS.gene03036.t1 AT1G02940 47.087 206 108 1 5 209 65 270 3.54e-66 205
MS.gene03036.t1 AT1G02940 47.087 206 108 1 5 209 53 258 4.34e-66 205
MS.gene03036.t1 AT3G62760 47.826 207 107 1 3 208 2 208 2.80e-63 196
MS.gene03036.t1 AT1G49860 47.692 195 98 2 21 211 22 216 7.64e-58 183
MS.gene03036.t1 AT3G03190 44.498 209 115 1 4 211 3 211 5.66e-57 180
MS.gene03036.t1 AT5G17220 39.234 209 126 1 4 211 3 211 6.49e-52 167
MS.gene03036.t1 AT2G30860 40.385 208 122 2 4 210 3 209 5.72e-50 162
MS.gene03036.t1 AT2G30870 38.424 203 124 1 9 210 7 209 2.74e-46 152
MS.gene03036.t1 AT1G02950 50.704 142 69 1 65 205 60 201 4.50e-44 147
MS.gene03036.t1 AT2G30860 37.500 152 94 1 4 155 3 153 5.86e-31 112
MS.gene03036.t1 AT5G41210 25.980 204 145 2 1 198 1 204 1.45e-11 62.4
MS.gene03036.t1 AT5G41240 29.208 202 131 5 4 196 3 201 3.80e-11 62.4

Find 54 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCATTATTGCTATCTTCT+TGG 0.169803 1.3:+62154493 None:intergenic
TTGGGGATAAAGAGTTTATT+TGG 0.186179 1.3:-62154410 MS.gene03036:CDS
ATCAAGAATGCTATCCAATT+TGG 0.237155 1.3:+62154349 None:intergenic
AGAGTCACCTCCCAAGTATT+TGG 0.266829 1.3:+62154301 None:intergenic
CTCCAAGAAGATAGCAATAA+TGG 0.269830 1.3:-62154495 MS.gene03036:CDS
TCAAAACTTGTTCGGGAATT+GGG 0.278093 1.3:-62154428 MS.gene03036:CDS
ATCAAAACTTGTTCGGGAAT+TGG 0.285433 1.3:-62154429 MS.gene03036:CDS
GATGGAGATTTAAAGCTCTT+TGG 0.296201 1.3:-62154859 MS.gene03036:intron
TGAAGAGAATGAAGCCAAAT+TGG 0.299438 1.3:-62154363 MS.gene03036:CDS
TCGTGATTCAAACAATTTCT+TGG 0.300130 1.3:+62154220 None:intergenic
GAAGAATGGAGAACACAAAA+AGG 0.349354 1.3:-62155089 MS.gene03036:CDS
GGAGTCATTGCTTGTTAACT+TGG 0.352335 1.3:+62154514 None:intergenic
CGCATGGTGGCCGTTGAGTA+GGG 0.361457 1.3:+62155169 None:intergenic
AACAATGAGTATGCTGATAA+GGG 0.368137 1.3:-62154539 MS.gene03036:CDS
TTCTTTATGTTGCAGCCATT+TGG 0.379956 1.3:-62154901 MS.gene03036:intron
TAACAATGAGTATGCTGATA+AGG 0.387390 1.3:-62154540 MS.gene03036:CDS
AGTAGCATCAAAACTTGTTC+GGG 0.393847 1.3:-62154435 MS.gene03036:CDS
AAGTAGCATCAAAACTTGTT+CGG 0.398448 1.3:-62154436 MS.gene03036:CDS
GAGTTTATTTGGAATTCCTT+TGG 0.399412 1.3:-62154399 MS.gene03036:CDS
CCGCATGGTGGCCGTTGAGT+AGG 0.403862 1.3:+62155168 None:intergenic
TCCAAGAAGATAGCAATAAT+GGG 0.404293 1.3:-62154494 MS.gene03036:CDS
GTTAACATTAACTTGAAGAA+TGG 0.418899 1.3:-62155103 MS.gene03036:CDS
AGGTGACTCTTTCACCTTGG+TGG 0.445525 1.3:-62154288 MS.gene03036:CDS
AAATCTCCATCTTCAAAAGC+TGG 0.446504 1.3:+62154871 None:intergenic
ACTGTCTTAATTAGGTATCA+TGG 0.449391 1.3:+62154136 None:intergenic
GCTTACATTGAGTGATATGA+AGG 0.454820 1.3:+62155063 None:intergenic
CACGATCTCATGTTAGTGCT+TGG 0.456189 1.3:-62154202 MS.gene03036:CDS
AAAATTGAAATGCAGAATCA+AGG 0.457301 1.3:-62154580 MS.gene03036:intron
ATTGTCTGAGTCCAAATACT+TGG 0.458455 1.3:-62154312 MS.gene03036:CDS
AAAGCTGGAACTTGACCAAA+TGG 0.480424 1.3:+62154886 None:intergenic
ACGATCTCATGTTAGTGCTT+GGG 0.482947 1.3:-62154201 MS.gene03036:CDS
ATGCTACTTGATCGAAGTGT+TGG 0.510802 1.3:+62154450 None:intergenic
GGGAGGTGACTCTTTCACCT+TGG 0.520363 1.3:-62154291 MS.gene03036:CDS
TTCATCAAGTAATGAAAAGT+AGG 0.527654 1.3:+62154251 None:intergenic
CTACTCAACGGCCACCATGC+GGG 0.529590 1.3:-62155167 MS.gene03036:CDS
CAAAACTTGTTCGGGAATTG+GGG 0.535983 1.3:-62154427 MS.gene03036:CDS
CTTAATTAGGTATCATGGCA+AGG 0.566951 1.3:+62154141 None:intergenic
AAATTGAAATGCAGAATCAA+GGG 0.575285 1.3:-62154579 MS.gene03036:intron
GCAAGGACTTTAGACCAAGC+TGG 0.580505 1.3:+62154158 None:intergenic
GCATGGTGGCCGTTGAGTAG+GGG 0.589594 1.3:+62155170 None:intergenic
ATATCACTGCAAGACCAGCT+TGG 0.601324 1.3:-62154172 MS.gene03036:CDS
TTGTCTGAGTCCAAATACTT+GGG 0.601763 1.3:-62154311 MS.gene03036:CDS
ATCTCATGTTAGTGCTTGGG+TGG 0.606175 1.3:-62154198 MS.gene03036:CDS
CCAAGAAGATAGCAATAATG+GGG 0.612634 1.3:-62154493 MS.gene03036:CDS
CTTACATTGAGTGATATGAA+GGG 0.613061 1.3:+62155064 None:intergenic
GCACGGAAGCCCCTACTCAA+CGG 0.627204 1.3:-62155179 MS.gene03036:CDS
ACAATGAGTATGCTGATAAG+GGG 0.629847 1.3:-62154538 MS.gene03036:CDS
ATGGCATCAATAAAAGTGCA+CGG 0.642318 1.3:-62155196 MS.gene03036:CDS
GAGAGTTGCAGTAACCCGCA+TGG 0.650972 1.3:+62155153 None:intergenic
CCTACTCAACGGCCACCATG+CGG 0.660998 1.3:-62155168 MS.gene03036:CDS
TCTGAGTCCAAATACTTGGG+AGG 0.681272 1.3:-62154308 MS.gene03036:CDS
AGTTGCAGTAACCCGCATGG+TGG 0.691159 1.3:+62155156 None:intergenic
CAAGAAGATAGCAATAATGG+GGG 0.701769 1.3:-62154492 MS.gene03036:CDS
TAAATGATGCAAATCCACCA+AGG 0.702241 1.3:+62154274 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATACATTGATAATTTAACAT+TGG + chr1.3:62154629-62154648 None:intergenic 15.0%
! AAAATTGAAATGCAGAATCA+AGG - chr1.3:62154758-62154777 MS.gene03036:intron 25.0%
! AAATTGAAATGCAGAATCAA+GGG - chr1.3:62154759-62154778 MS.gene03036:intron 25.0%
! TTCATCAAGTAATGAAAAGT+AGG + chr1.3:62155090-62155109 None:intergenic 25.0%
!! ATCAATTAGTTTGATCAATG+TGG + chr1.3:62154414-62154433 None:intergenic 25.0%
!! GTTAACATTAACTTGAAGAA+TGG - chr1.3:62154235-62154254 MS.gene03036:CDS 25.0%
!! TCATTCTCTTCAACAATTTT+TGG + chr1.3:62154967-62154986 None:intergenic 25.0%
AACAATGAGTATGCTGATAA+GGG - chr1.3:62154799-62154818 MS.gene03036:intron 30.0%
ATCAAGAATGCTATCCAATT+TGG + chr1.3:62154992-62155011 None:intergenic 30.0%
CTTACATTGAGTGATATGAA+GGG + chr1.3:62154277-62154296 None:intergenic 30.0%
TAACAATGAGTATGCTGATA+AGG - chr1.3:62154798-62154817 MS.gene03036:intron 30.0%
TCCAAGAAGATAGCAATAAT+GGG - chr1.3:62154844-62154863 MS.gene03036:intron 30.0%
TCGTGATTCAAACAATTTCT+TGG + chr1.3:62155121-62155140 None:intergenic 30.0%
TTGGGGATAAAGAGTTTATT+TGG - chr1.3:62154928-62154947 MS.gene03036:intron 30.0%
! AAGTAGCATCAAAACTTGTT+CGG - chr1.3:62154902-62154921 MS.gene03036:intron 30.0%
! ATTAGTTTGATCAATGTGGT+TGG + chr1.3:62154410-62154429 None:intergenic 30.0%
! GAGTTTATTTGGAATTCCTT+TGG - chr1.3:62154939-62154958 MS.gene03036:intron 30.0%
!! TCAACAATTTTTGGATCCAA+AGG + chr1.3:62154958-62154977 None:intergenic 30.0%
!!! GTTTCTTTTCATTTTTTGCG+AGG + chr1.3:62154585-62154604 None:intergenic 30.0%
AAATCTCCATCTTCAAAAGC+TGG + chr1.3:62154470-62154489 None:intergenic 35.0%
ACAATGAGTATGCTGATAAG+GGG - chr1.3:62154800-62154819 MS.gene03036:intron 35.0%
ATTGTCTGAGTCCAAATACT+TGG - chr1.3:62155026-62155045 MS.gene03036:intron 35.0%
CAAGAAGATAGCAATAATGG+GGG - chr1.3:62154846-62154865 MS.gene03036:intron 35.0%
CCAAGAAGATAGCAATAATG+GGG - chr1.3:62154845-62154864 MS.gene03036:intron 35.0%
CTCCAAGAAGATAGCAATAA+TGG - chr1.3:62154843-62154862 MS.gene03036:intron 35.0%
GAAGAATGGAGAACACAAAA+AGG - chr1.3:62154249-62154268 MS.gene03036:CDS 35.0%
GCTTACATTGAGTGATATGA+AGG + chr1.3:62154278-62154297 None:intergenic 35.0%
TAAATGATGCAAATCCACCA+AGG + chr1.3:62155067-62155086 None:intergenic 35.0%
TGAAGAGAATGAAGCCAAAT+TGG - chr1.3:62154975-62154994 MS.gene03036:intron 35.0%
TTCTTTATGTTGCAGCCATT+TGG - chr1.3:62154437-62154456 MS.gene03036:CDS 35.0%
TTGTCTGAGTCCAAATACTT+GGG - chr1.3:62155027-62155046 MS.gene03036:intron 35.0%
! AGTAGCATCAAAACTTGTTC+GGG - chr1.3:62154903-62154922 MS.gene03036:intron 35.0%
! ATCAAAACTTGTTCGGGAAT+TGG - chr1.3:62154909-62154928 MS.gene03036:intron 35.0%
! ATGGCATCAATAAAAGTGCA+CGG - chr1.3:62154142-62154161 MS.gene03036:CDS 35.0%
! GATGGAGATTTAAAGCTCTT+TGG - chr1.3:62154479-62154498 MS.gene03036:CDS 35.0%
! TCAAAACTTGTTCGGGAATT+GGG - chr1.3:62154910-62154929 MS.gene03036:intron 35.0%
! TTTTTGCGAGGAGTGAAATT+AGG + chr1.3:62154573-62154592 None:intergenic 35.0%
AAAGCTGGAACTTGACCAAA+TGG + chr1.3:62154455-62154474 None:intergenic 40.0%
ACGATCTCATGTTAGTGCTT+GGG - chr1.3:62155137-62155156 MS.gene03036:CDS 40.0%
CCCCATTATTGCTATCTTCT+TGG + chr1.3:62154848-62154867 None:intergenic 40.0%
TCAATGTGGTTGGAACAAGA+AGG + chr1.3:62154400-62154419 None:intergenic 40.0%
TGTCATGTGCTACGTAAATC+AGG + chr1.3:62154520-62154539 None:intergenic 40.0%
! AGATAGCAATAATGGGGGTA+TGG - chr1.3:62154851-62154870 MS.gene03036:intron 40.0%
! ATGCTACTTGATCGAAGTGT+TGG + chr1.3:62154891-62154910 None:intergenic 40.0%
! CAAAACTTGTTCGGGAATTG+GGG - chr1.3:62154911-62154930 MS.gene03036:intron 40.0%
! GGAGTCATTGCTTGTTAACT+TGG + chr1.3:62154827-62154846 None:intergenic 40.0%
!! CAAGTTCCAGCTTTTGAAGA+TGG - chr1.3:62154461-62154480 MS.gene03036:CDS 40.0%
AGAGTCACCTCCCAAGTATT+TGG + chr1.3:62155040-62155059 None:intergenic 45.0%
ATATCACTGCAAGACCAGCT+TGG - chr1.3:62155166-62155185 MS.gene03036:CDS 45.0%
ATCTCATGTTAGTGCTTGGG+TGG - chr1.3:62155140-62155159 MS.gene03036:CDS 45.0%
CACGATCTCATGTTAGTGCT+TGG - chr1.3:62155136-62155155 MS.gene03036:CDS 45.0%
CTAATATTTGTGCGAGCGCT+TGG - chr1.3:62154706-62154725 MS.gene03036:intron 45.0%
TCTGAGTCCAAATACTTGGG+AGG - chr1.3:62155030-62155049 MS.gene03036:intron 45.0%
AGGTGACTCTTTCACCTTGG+TGG - chr1.3:62155050-62155069 MS.gene03036:intron 50.0%
GCAAGGACTTTAGACCAAGC+TGG + chr1.3:62155183-62155202 None:intergenic 50.0%
! AATGGGGGTATGGTCTGAAG+TGG - chr1.3:62154861-62154880 MS.gene03036:CDS 50.0%
AGTTGCAGTAACCCGCATGG+TGG + chr1.3:62154185-62154204 None:intergenic 55.0%
GAGAGTTGCAGTAACCCGCA+TGG + chr1.3:62154188-62154207 None:intergenic 55.0%
GGGAGGTGACTCTTTCACCT+TGG - chr1.3:62155047-62155066 MS.gene03036:intron 55.0%
CCTACTCAACGGCCACCATG+CGG - chr1.3:62154170-62154189 MS.gene03036:CDS 60.0%
CGCATGGTGGCCGTTGAGTA+GGG + chr1.3:62154172-62154191 None:intergenic 60.0%
CTACTCAACGGCCACCATGC+GGG - chr1.3:62154171-62154190 MS.gene03036:CDS 60.0%
GCACGGAAGCCCCTACTCAA+CGG - chr1.3:62154159-62154178 MS.gene03036:CDS 60.0%
GCATGGTGGCCGTTGAGTAG+GGG + chr1.3:62154171-62154190 None:intergenic 60.0%
CCGCATGGTGGCCGTTGAGT+AGG + chr1.3:62154173-62154192 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 62154142 62155218 62154142 ID=MS.gene03036
chr1.3 mRNA 62154142 62155218 62154142 ID=MS.gene03036.t1;Parent=MS.gene03036
chr1.3 exon 62155069 62155218 62155069 ID=MS.gene03036.t1.exon1;Parent=MS.gene03036.t1
chr1.3 CDS 62155069 62155218 62155069 ID=cds.MS.gene03036.t1;Parent=MS.gene03036.t1
chr1.3 exon 62154860 62154908 62154860 ID=MS.gene03036.t1.exon2;Parent=MS.gene03036.t1
chr1.3 CDS 62154860 62154908 62154860 ID=cds.MS.gene03036.t1;Parent=MS.gene03036.t1
chr1.3 exon 62154142 62154587 62154142 ID=MS.gene03036.t1.exon3;Parent=MS.gene03036.t1
chr1.3 CDS 62154142 62154587 62154142 ID=cds.MS.gene03036.t1;Parent=MS.gene03036.t1
Gene Sequence

>MS.gene03036

ATGGCATCAATAAAAGTGCACGGAAGCCCCTACTCAACGGCCACCATGCGGGTTACTGCAACTCTCTATGAAAAACAGATTGAGTTTGAGTTTGTTAACATTAACTTGAAGAATGGAGAACACAAAAAGGAACCCTTCATATCACTCAATGTAAGCAATATAAAAATATATATATATATATATATATATATATATATATATATATATATATATATATCGTTCTGCTAGTTCACTTCAATTATGAATTGAAATTTTCCTTCTTGTTCCAACCACATTGATCAAACTAATTGATTAATTCTTTATGTTGCAGCCATTTGGTCAAGTTCCAGCTTTTGAAGATGGAGATTTAAAGCTCTTTGGTAAAATTTATATGAACCTGATTTACGTAGCACATGACACTTTAGATTAAACATTAATTATGTGTATTTCCTAATTTCACTCCTCGCAAAAAATGAAAAGAAACAAAAAAAAAAAACATTTTTTACCAATGTTAAATTATCAATGTATGTATCGATATGTCAATATGTGTATGTATCGATATGTCAATGTATGTATCGTGTCTCTCTAATATTTGTGCGAGCGCTTGGTAGAATATGATGTAGATATAAACTAATTAAAAATTGAAATGCAGAATCAAGGGCGATAACACAATATATTAACAATGAGTATGCTGATAAGGGGACCAAGTTAACAAGCAATGACTCCAAGAAGATAGCAATAATGGGGGTATGGTCTGAAGTGGAGAGCCAACACTTCGATCAAGTAGCATCAAAACTTGTTCGGGAATTGGGGATAAAGAGTTTATTTGGAATTCCTTTGGATCCAAAAATTGTTGAAGAGAATGAAGCCAAATTGGATAGCATTCTTGATGTGTATGAGAAAAGATTGTCTGAGTCCAAATACTTGGGAGGTGACTCTTTCACCTTGGTGGATTTGCATCATTTACCTACTTTTCATTACTTGATGAAGTCACAGACCAAGAAATTGTTTGAATCACGATCTCATGTTAGTGCTTGGGTGGCTGATATCACTGCAAGACCAGCTTGGTCTAAAGTCCTTGCCATGATACCTAATTAA

Protein sequence

>MS.gene03036.t1

MASIKVHGSPYSTATMRVTATLYEKQIEFEFVNINLKNGEHKKEPFISLNPFGQVPAFEDGDLKLFESRAITQYINNEYADKGTKLTSNDSKKIAIMGVWSEVESQHFDQVASKLVRELGIKSLFGIPLDPKIVEENEAKLDSILDVYEKRLSESKYLGGDSFTLVDLHHLPTFHYLMKSQTKKLFESRSHVSAWVADITARPAWSKVLAMIPN