AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07192

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07192.t1 MTR_1g088850 97.727 220 5 0 1 220 1 220 1.64e-164 452
MS.gene07192.t1 MTR_3g450790 48.571 210 105 3 1 209 1 208 2.56e-69 211
MS.gene07192.t1 MTR_3g450930 45.714 210 111 3 1 209 1 208 8.24e-63 195
MS.gene07192.t1 MTR_5g090920 44.076 211 114 4 1 209 1 209 1.25e-56 179
MS.gene07192.t1 MTR_1g088845 40.865 208 122 1 3 210 4 210 1.43e-56 179
MS.gene07192.t1 MTR_3g064700 43.062 209 117 2 1 208 1 208 3.60e-56 178
MS.gene07192.t1 MTR_1g088840 39.423 208 125 1 3 210 4 210 1.82e-55 176
MS.gene07192.t1 MTR_1g088825 39.423 208 125 1 3 210 4 210 1.82e-55 176
MS.gene07192.t1 MTR_5g090910 42.453 212 116 5 1 209 1 209 2.25e-54 173
MS.gene07192.t1 MTR_1g026140 37.736 212 127 1 3 209 5 216 5.13e-51 165
MS.gene07192.t1 MTR_1g088840 39.506 162 97 1 49 210 3 163 5.51e-41 137
MS.gene07192.t1 MTR_5g090910 41.497 147 81 4 66 209 8 152 2.21e-29 107
MS.gene07192.t1 MTR_1g088840 37.500 112 70 0 99 210 3 114 1.60e-24 94.0
MS.gene07192.t1 MTR_4g134770 26.316 228 144 5 1 219 1 213 2.92e-14 71.2
MS.gene07192.t1 MTR_3g058940 27.358 212 143 5 1 210 1 203 1.26e-13 69.3
MS.gene07192.t1 MTR_1g492670 42.188 64 37 0 147 210 2 65 2.58e-12 60.5
MS.gene07192.t1 MTR_2g005570 27.053 207 130 5 11 210 13 205 8.14e-12 63.9
MS.gene07192.t1 MTR_2g005570 27.184 206 131 5 11 210 13 205 1.07e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07192.t1 AT3G62760 56.221 217 95 0 1 217 1 217 6.28e-96 279
MS.gene07192.t1 AT1G02930 41.827 208 119 1 2 209 3 208 2.08e-58 183
MS.gene07192.t1 AT1G02930 41.827 208 119 1 2 209 3 208 2.08e-58 183
MS.gene07192.t1 AT1G02920 41.346 208 121 1 2 209 3 209 2.23e-56 178
MS.gene07192.t1 AT2G30860 43.721 215 118 3 1 214 1 213 3.27e-55 176
MS.gene07192.t1 AT2G47730 41.951 205 119 0 5 209 54 258 2.08e-54 175
MS.gene07192.t1 AT2G47730 41.951 205 119 0 5 209 54 258 2.08e-54 175
MS.gene07192.t1 AT4G02520 40.476 210 121 2 2 208 3 211 1.64e-53 171
MS.gene07192.t1 AT2G30870 43.541 209 115 3 1 208 1 207 7.88e-53 169
MS.gene07192.t1 AT2G02930 39.524 210 125 1 2 209 3 212 9.76e-53 169
MS.gene07192.t1 AT1G02950 35.437 206 133 0 1 206 23 228 2.69e-49 161
MS.gene07192.t1 AT1G02950 35.437 206 133 0 1 206 23 228 2.69e-49 161
MS.gene07192.t1 AT3G03190 37.619 210 129 2 1 209 1 209 4.03e-49 160
MS.gene07192.t1 AT1G02950 35.437 206 133 0 1 206 35 240 4.63e-49 161
MS.gene07192.t1 AT1G02950 35.961 203 130 0 4 206 28 230 5.62e-49 160
MS.gene07192.t1 AT1G49860 41.500 200 112 3 14 209 16 214 1.10e-47 157
MS.gene07192.t1 AT5G17220 33.333 210 138 2 1 209 1 209 5.72e-46 152
MS.gene07192.t1 AT1G02940 36.408 206 131 0 4 209 40 245 1.77e-45 152
MS.gene07192.t1 AT1G02940 36.408 206 131 0 4 209 40 245 1.77e-45 152
MS.gene07192.t1 AT1G02940 36.408 206 131 0 4 209 53 258 2.28e-45 152
MS.gene07192.t1 AT1G02940 36.408 206 131 0 4 209 65 270 2.69e-45 152
MS.gene07192.t1 AT2G30860 40.000 155 91 2 1 155 1 153 3.67e-32 115
MS.gene07192.t1 AT1G02950 30.070 143 100 0 64 206 60 202 7.20e-26 100
MS.gene07192.t1 AT1G09640 26.415 212 146 5 1 210 1 204 1.10e-11 63.9
MS.gene07192.t1 AT1G57720 26.606 218 138 7 1 210 1 204 1.20e-11 63.5
MS.gene07192.t1 AT1G57720 26.606 218 138 7 1 210 1 204 1.20e-11 63.5
MS.gene07192.t1 AT1G09640 26.415 212 146 5 1 210 1 204 1.47e-11 62.8

Find 44 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTGACCTGTTTGGTTCT+TGG 0.194862 1.4:-70520160 None:intergenic
ATCATGGCACTCAAGATTTA+TGG 0.277244 1.4:+70519369 None:intergenic
AATAGTGAGTTGAAGATATA+TGG 0.317000 1.4:-70520289 None:intergenic
ACAAAGTATTCATAGATAAT+TGG 0.322317 1.4:-70520122 None:intergenic
TTCAGCTACATATGAGGTTA+TGG 0.327058 1.4:-70519988 None:intergenic
GATGATGATCTCACCCTCTT+TGG 0.366830 1.4:+70519621 MS.gene07192:CDS
CTTTCAATCACTGACCTGTT+TGG 0.367204 1.4:-70520167 None:intergenic
CAATGAACGGATTCATGTTA+AGG 0.371100 1.4:+70520340 MS.gene07192:CDS
TTTGGTTCTTGGCCTTGAAA+TGG 0.382658 1.4:-70520149 None:intergenic
AAAATGGACCTGATTTGATT+AGG 0.390014 1.4:+70520021 MS.gene07192:CDS
TACTTTGTGGTTCCATTTCA+AGG 0.391654 1.4:+70520137 MS.gene07192:CDS
GTTGTGGGAGGTATGACTTT+TGG 0.408716 1.4:+70520404 MS.gene07192:CDS
CTTACGTTCTTGTTGAGAAA+AGG 0.412482 1.4:-70519501 None:intergenic
AGCACAACAAAGTACCTTGC+AGG 0.416067 1.4:+70520239 MS.gene07192:CDS
GGTGGGAAGATATATCTTCT+AGG 0.418080 1.4:+70520366 MS.gene07192:CDS
TCTTTGTTCATGCAGCCCTT+TGG 0.422040 1.4:+70519579 MS.gene07192:intron
AATTATCTATGAATACTTTG+TGG 0.442647 1.4:+70520124 MS.gene07192:CDS
TGACACAAAAGAAGCTTCAT+TGG 0.444080 1.4:+70520049 MS.gene07192:CDS
TAGATAATTGGTGTGACTGC+AGG 0.445936 1.4:-70520110 None:intergenic
GTACTTTGTTGTGCTTAGCT+TGG 0.454755 1.4:-70520231 None:intergenic
AGATCATCATCTTCAAGTAC+TGG 0.467902 1.4:-70519609 None:intergenic
GGCACTCAAGATTTATGGTC+TGG 0.469062 1.4:+70519374 MS.gene07192:CDS
TTCAAGGCCAAGAACCAAAC+AGG 0.470857 1.4:+70520153 MS.gene07192:CDS
TCATTGTGCCTAATCAAATC+AGG 0.483923 1.4:-70520029 None:intergenic
AACGGATTCATGTTAAGGCT+TGG 0.491423 1.4:+70520345 MS.gene07192:CDS
ACGAGTGGTGTTTGTGGACA+TGG 0.514578 1.4:-70519397 None:intergenic
CATGGCACGAGTGGTGTTTG+TGG 0.516433 1.4:-70519403 None:intergenic
GATTCATGTTAAGGCTTGGT+GGG 0.536813 1.4:+70520349 MS.gene07192:CDS
GGATTCATGTTAAGGCTTGG+TGG 0.539482 1.4:+70520348 MS.gene07192:CDS
GCACAACAAAGTACCTTGCA+GGG 0.549960 1.4:+70520240 MS.gene07192:CDS
AGATAATTGGTGTGACTGCA+GGG 0.554379 1.4:-70520109 None:intergenic
AAAAGAAAGCATACCAAAGA+GGG 0.568007 1.4:-70519634 None:intergenic
TCAGAAGCAAACACATTGAC+TGG 0.571377 1.4:-70519465 None:intergenic
ACGTTCTTGTTGAGAAAAGG+AGG 0.590052 1.4:-70519498 None:intergenic
AGTACTGGAATCAAACCAAA+GGG 0.590562 1.4:-70519594 None:intergenic
AAGCTTCATTGGTCAAAATG+TGG 0.591373 1.4:+70520060 MS.gene07192:CDS
GTGCTTCAATGATCAATGAA+CGG 0.607609 1.4:+70520327 MS.gene07192:CDS
ATTGATCATTGAAGCACAAG+GGG 0.612739 1.4:-70520321 None:intergenic
AAGTACTGGAATCAAACCAA+AGG 0.617639 1.4:-70519595 None:intergenic
TCATTGATCATTGAAGCACA+AGG 0.633784 1.4:-70520323 None:intergenic
CATTGATCATTGAAGCACAA+GGG 0.636560 1.4:-70520322 None:intergenic
CACAACAAAGTACCTTGCAG+GGG 0.682868 1.4:+70520241 MS.gene07192:CDS
TAGACATATCATGGCACGAG+TGG 0.683620 1.4:-70519412 None:intergenic
ATTGGTCAAAATGTGGACAG+AGG 0.694356 1.4:+70520067 MS.gene07192:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTCGAATAAACTTAAA+GGG - chr1.4:70519699-70519718 None:intergenic 15.0%
!!! TATTTTTCGAATAAACTTAA+AGG - chr1.4:70519700-70519719 None:intergenic 15.0%
!! AATTATCTATGAATACTTTG+TGG + chr1.4:70520124-70520143 MS.gene07192:CDS 20.0%
!! GAATTAGTAACAAAAATTAG+AGG - chr1.4:70519833-70519852 None:intergenic 20.0%
!!! ACAAAGTATTCATAGATAAT+TGG - chr1.4:70520125-70520144 None:intergenic 20.0%
! AAAAGGTTAGATATCATGAA+AGG - chr1.4:70519931-70519950 None:intergenic 25.0%
! AAAGGTTAGATATCATGAAA+GGG - chr1.4:70519930-70519949 None:intergenic 25.0%
! AATCAAGCAAAAATACTACT+AGG + chr1.4:70519747-70519766 MS.gene07192:intron 25.0%
! ATATGTAGCTGAAAAATACA+AGG + chr1.4:70519998-70520017 MS.gene07192:CDS 25.0%
!! AATAGTGAGTTGAAGATATA+TGG - chr1.4:70520292-70520311 None:intergenic 25.0%
!! TTTTTCATGTAGACATATCA+TGG - chr1.4:70519424-70519443 None:intergenic 25.0%
!!! TAACCTTTTTTATGTGACTA+TGG + chr1.4:70519942-70519961 MS.gene07192:intron 25.0%
AGTCCATAGTCACATAAAAA+AGG - chr1.4:70519948-70519967 None:intergenic 30.0%
GCTGAAAAATACAAGGAAAA+TGG + chr1.4:70520005-70520024 MS.gene07192:CDS 30.0%
TTTCGAATAAACTTAAAGGG+TGG - chr1.4:70519696-70519715 None:intergenic 30.0%
! AAAAAGAAAGCATACCAAAG+AGG - chr1.4:70519638-70519657 None:intergenic 30.0%
! AAAAGAAAGCATACCAAAGA+GGG - chr1.4:70519637-70519656 None:intergenic 30.0%
! AAAATGGACCTGATTTGATT+AGG + chr1.4:70520021-70520040 MS.gene07192:CDS 30.0%
!! GTATTTTTCAGCTACATATG+AGG - chr1.4:70519997-70520016 None:intergenic 30.0%
AAGCTTCATTGGTCAAAATG+TGG + chr1.4:70520060-70520079 MS.gene07192:CDS 35.0%
AGATCATCATCTTCAAGTAC+TGG - chr1.4:70519612-70519631 None:intergenic 35.0%
ATTGATCATTGAAGCACAAG+GGG - chr1.4:70520324-70520343 None:intergenic 35.0%
CATTGATCATTGAAGCACAA+GGG - chr1.4:70520325-70520344 None:intergenic 35.0%
CTTACGTTCTTGTTGAGAAA+AGG - chr1.4:70519504-70519523 None:intergenic 35.0%
GCATGATTAGTGGTTTAGAT+TGG - chr1.4:70519864-70519883 None:intergenic 35.0%
GTGCTTCAATGATCAATGAA+CGG + chr1.4:70520327-70520346 MS.gene07192:CDS 35.0%
TACTTTGTGGTTCCATTTCA+AGG + chr1.4:70520137-70520156 MS.gene07192:CDS 35.0%
TCATTGATCATTGAAGCACA+AGG - chr1.4:70520326-70520345 None:intergenic 35.0%
TCATTGTGCCTAATCAAATC+AGG - chr1.4:70520032-70520051 None:intergenic 35.0%
TCTGCTTTCCAAAAAGTTGT+GGG + chr1.4:70520389-70520408 MS.gene07192:CDS 35.0%
TGACACAAAAGAAGCTTCAT+TGG + chr1.4:70520049-70520068 MS.gene07192:CDS 35.0%
TTCAGCTACATATGAGGTTA+TGG - chr1.4:70519991-70520010 None:intergenic 35.0%
! AGTACTGGAATCAAACCAAA+GGG - chr1.4:70519597-70519616 None:intergenic 35.0%
! CAATGAACGGATTCATGTTA+AGG + chr1.4:70520340-70520359 MS.gene07192:CDS 35.0%
!! AAGTACTGGAATCAAACCAA+AGG - chr1.4:70519598-70519617 None:intergenic 35.0%
ACGTTCTTGTTGAGAAAAGG+AGG - chr1.4:70519501-70519520 None:intergenic 40.0%
AGATAATTGGTGTGACTGCA+GGG - chr1.4:70520112-70520131 None:intergenic 40.0%
ATTGGTCAAAATGTGGACAG+AGG + chr1.4:70520067-70520086 MS.gene07192:CDS 40.0%
CTTTCAATCACTGACCTGTT+TGG - chr1.4:70520170-70520189 None:intergenic 40.0%
GACTATAAAAATCCCCTGCA+AGG - chr1.4:70520256-70520275 None:intergenic 40.0%
GGTGGGAAGATATATCTTCT+AGG + chr1.4:70520366-70520385 MS.gene07192:CDS 40.0%
GTCTGCTTTCCAAAAAGTTG+TGG + chr1.4:70520388-70520407 MS.gene07192:CDS 40.0%
TAGATAATTGGTGTGACTGC+AGG - chr1.4:70520113-70520132 None:intergenic 40.0%
TCAGAAGCAAACACATTGAC+TGG - chr1.4:70519468-70519487 None:intergenic 40.0%
TGACATGCATGCATGATTAG+TGG - chr1.4:70519874-70519893 None:intergenic 40.0%
! AACGGATTCATGTTAAGGCT+TGG + chr1.4:70520345-70520364 MS.gene07192:CDS 40.0%
! GATTCATGTTAAGGCTTGGT+GGG + chr1.4:70520349-70520368 MS.gene07192:CDS 40.0%
! GTACTTTGTTGTGCTTAGCT+TGG - chr1.4:70520234-70520253 None:intergenic 40.0%
! TCATACCTCCCACAACTTTT+TGG - chr1.4:70520400-70520419 None:intergenic 40.0%
!! TTTGGTTCTTGGCCTTGAAA+TGG - chr1.4:70520152-70520171 None:intergenic 40.0%
GATGATGATCTCACCCTCTT+TGG + chr1.4:70519621-70519640 MS.gene07192:CDS 45.0%
GCTTTCCAAAAAGTTGTGGG+AGG + chr1.4:70520392-70520411 MS.gene07192:CDS 45.0%
GGATTCATGTTAAGGCTTGG+TGG + chr1.4:70520348-70520367 MS.gene07192:CDS 45.0%
GGCACTCAAGATTTATGGTC+TGG + chr1.4:70519374-70519393 MS.gene07192:CDS 45.0%
TAGACATATCATGGCACGAG+TGG - chr1.4:70519415-70519434 None:intergenic 45.0%
TCTTTGTTCATGCAGCCCTT+TGG + chr1.4:70519579-70519598 MS.gene07192:intron 45.0%
TTCAAGGCCAAGAACCAAAC+AGG + chr1.4:70520153-70520172 MS.gene07192:CDS 45.0%
! AGCACAACAAAGTACCTTGC+AGG + chr1.4:70520239-70520258 MS.gene07192:CDS 45.0%
! CACAACAAAGTACCTTGCAG+GGG + chr1.4:70520241-70520260 MS.gene07192:CDS 45.0%
! GCACAACAAAGTACCTTGCA+GGG + chr1.4:70520240-70520259 MS.gene07192:CDS 45.0%
!! GTTGTGGGAGGTATGACTTT+TGG + chr1.4:70520404-70520423 MS.gene07192:CDS 45.0%
!! TCACTGACCTGTTTGGTTCT+TGG - chr1.4:70520163-70520182 None:intergenic 45.0%
ACGAGTGGTGTTTGTGGACA+TGG - chr1.4:70519400-70519419 None:intergenic 50.0%
CATGGCACGAGTGGTGTTTG+TGG - chr1.4:70519406-70519425 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 70519372 70520445 70519372 ID=MS.gene07192
chr1.4 mRNA 70519372 70520445 70519372 ID=MS.gene07192.t1;Parent=MS.gene07192
chr1.4 exon 70519372 70519518 70519372 ID=MS.gene07192.t1.exon1;Parent=MS.gene07192.t1
chr1.4 CDS 70519372 70519518 70519372 ID=cds.MS.gene07192.t1;Parent=MS.gene07192.t1
chr1.4 exon 70519594 70519642 70519594 ID=MS.gene07192.t1.exon2;Parent=MS.gene07192.t1
chr1.4 CDS 70519594 70519642 70519594 ID=cds.MS.gene07192.t1;Parent=MS.gene07192.t1
chr1.4 exon 70519979 70520445 70519979 ID=MS.gene07192.t1.exon3;Parent=MS.gene07192.t1
chr1.4 CDS 70519979 70520445 70519979 ID=cds.MS.gene07192.t1;Parent=MS.gene07192.t1
Gene Sequence

>MS.gene07192

ATGGCACTCAAGATTTATGGTCTGGCCATGTCCACAAACACCACTCGTGCCATGATATGTCTACATGAAAAAGAAGTAGATTTTGAGCTTATTCCAGTCAATGTGTTTGCTTCTGAGCATAAACAGCCTCCTTTTCTCAACAAGAACGTAAGTAAAAAAAGCACTTCTAGCTAGCTTATATATCTTTTCTTAATCACATACTAATTTTCTTTGTTCATGCAGCCCTTTGGTTTGATTCCAGTACTTGAAGATGATGATCTCACCCTCTTTGGTATGCTTTCTTTTTTTCTTTTTCAGTTGTTACATTTTATTCACTCTGCACCACCCTTTAAGTTTATTCGAAAAATATACTTTGATGCTCGCTTTCATATTTATAATCAAGCAAAAATACTACTAGGAAAAAAAAAATTATGTAATTTCAGCGCGAGAAGTGACTCTGAAATTTGAGAATTTTTTGTCCTCTAATTTTTGTTACTAATTCAACGTTTTCCAATCTAAACCACTAATCATGCATGCATGTCAGATAATTTGATATTGTTTTGTTTCATTTTTTAACCCTTTCATGATATCTAACCTTTTTTATGTGACTATGGACTACACAAAAAAGAGTCAAGAGCCATAACCTCATATGTAGCTGAAAAATACAAGGAAAATGGACCTGATTTGATTAGGCACAATGACACAAAAGAAGCTTCATTGGTCAAAATGTGGACAGAGGTAGAGTCTCACTACTATGACCCTGCAGTCACACCAATTATCTATGAATACTTTGTGGTTCCATTTCAAGGCCAAGAACCAAACAGGTCAGTGATTGAAAGTAACATTGAGAAGCTGAAGATTGTGCTTGATGTGTATGAGACCAAGCTAAGCACAACAAAGTACCTTGCAGGGGATTTTTATAGTCTTGCTGATCTTAGCCATATATCTTCAACTCACTATTTTATGCAGACCCCTTGTGCTTCAATGATCAATGAACGGATTCATGTTAAGGCTTGGTGGGAAGATATATCTTCTAGGTCTGCTTTCCAAAAAGTTGTGGGAGGTATGACTTTTGGTCAGAGTGATCAAAAATGA
Protein sequence

>MS.gene07192.t1

MALKIYGLAMSTNTTRAMICLHEKEVDFELIPVNVFASEHKQPPFLNKNPFGLIPVLEDDDLTLFESRAITSYVAEKYKENGPDLIRHNDTKEASLVKMWTEVESHYYDPAVTPIIYEYFVVPFQGQEPNRSVIESNIEKLKIVLDVYETKLSTTKYLAGDFYSLADLSHISSTHYFMQTPCASMINERIHVKAWWEDISSRSAFQKVVGGMTFGQSDQK