AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07196


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07196.t1 MTR_1g088840 92.523 214 16 0 1 214 1 214 1.65e-149 414
MS.gene07196.t1 MTR_1g088825 92.523 214 16 0 1 214 1 214 1.65e-149 414
MS.gene07196.t1 MTR_1g088845 86.916 214 28 0 1 214 1 214 1.55e-139 389
MS.gene07196.t1 MTR_1g088840 91.566 166 14 0 49 214 2 167 3.83e-112 318
MS.gene07196.t1 MTR_1g088840 92.373 118 9 0 97 214 1 118 3.62e-77 228
MS.gene07196.t1 MTR_3g064700 46.117 206 110 1 4 208 3 208 3.00e-60 188
MS.gene07196.t1 MTR_3g450930 44.498 209 114 2 4 211 3 210 4.70e-59 185
MS.gene07196.t1 MTR_3g450790 44.019 209 115 2 4 211 3 210 3.63e-58 182
MS.gene07196.t1 MTR_1g088850 39.713 209 125 1 3 210 2 210 1.57e-56 179
MS.gene07196.t1 MTR_5g090920 44.019 209 112 4 4 209 3 209 8.61e-56 177
MS.gene07196.t1 MTR_1g026140 42.130 216 119 3 4 213 5 220 2.46e-55 176
MS.gene07196.t1 MTR_5g090910 41.905 210 119 3 4 211 3 211 1.30e-54 174
MS.gene07196.t1 MTR_1g492670 86.765 68 9 0 147 214 2 69 6.11e-39 129
MS.gene07196.t1 MTR_5g090910 40.136 147 86 2 67 211 8 154 3.59e-32 114
MS.gene07196.t1 MTR_3g088635 23.013 239 152 5 4 210 1 239 2.96e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07196.t1 AT1G02920 63.158 209 77 0 1 209 1 209 5.04e-89 261
MS.gene07196.t1 AT1G02930 61.722 209 79 1 1 209 1 208 6.37e-88 258
MS.gene07196.t1 AT1G02930 61.722 209 79 1 1 209 1 208 6.37e-88 258
MS.gene07196.t1 AT2G47730 58.049 205 85 1 6 209 54 258 3.82e-86 256
MS.gene07196.t1 AT2G47730 58.049 205 85 1 6 209 54 258 3.82e-86 256
MS.gene07196.t1 AT4G02520 59.242 211 83 1 1 208 1 211 6.27e-85 251
MS.gene07196.t1 AT2G02930 58.962 212 84 1 1 209 1 212 3.66e-83 246
MS.gene07196.t1 AT1G02950 54.369 206 93 1 1 205 24 229 5.48e-78 234
MS.gene07196.t1 AT1G02950 54.950 202 90 1 5 205 26 227 2.61e-77 232
MS.gene07196.t1 AT1G02950 54.950 202 90 1 5 205 26 227 2.61e-77 232
MS.gene07196.t1 AT1G02950 54.950 202 90 1 5 205 38 239 2.86e-77 233
MS.gene07196.t1 AT1G02940 47.087 206 108 1 5 209 40 245 2.56e-66 205
MS.gene07196.t1 AT1G02940 47.087 206 108 1 5 209 40 245 2.56e-66 205
MS.gene07196.t1 AT1G02940 47.087 206 108 1 5 209 65 270 3.54e-66 205
MS.gene07196.t1 AT1G02940 47.087 206 108 1 5 209 53 258 4.34e-66 205
MS.gene07196.t1 AT3G62760 47.826 207 107 1 3 208 2 208 2.80e-63 196
MS.gene07196.t1 AT1G49860 47.692 195 98 2 21 211 22 216 7.64e-58 183
MS.gene07196.t1 AT3G03190 44.498 209 115 1 4 211 3 211 5.66e-57 180
MS.gene07196.t1 AT5G17220 39.234 209 126 1 4 211 3 211 6.49e-52 167
MS.gene07196.t1 AT2G30860 40.385 208 122 2 4 210 3 209 5.72e-50 162
MS.gene07196.t1 AT2G30870 38.424 203 124 1 9 210 7 209 2.74e-46 152
MS.gene07196.t1 AT1G02950 50.704 142 69 1 65 205 60 201 4.50e-44 147
MS.gene07196.t1 AT2G30860 37.500 152 94 1 4 155 3 153 5.86e-31 112
MS.gene07196.t1 AT5G41210 25.980 204 145 2 1 198 1 204 1.45e-11 62.4
MS.gene07196.t1 AT5G41240 29.208 202 131 5 4 196 3 201 3.80e-11 62.4

Find 55 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCATTATTGCTATCTTCT+TGG 0.169803 1.4:+70486528 None:intergenic
TTGGGGATAAAGAGTTTATT+TGG 0.186179 1.4:-70486445 MS.gene07196:CDS
AGAGTCACCTCCCAAGTATT+TGG 0.266829 1.4:+70486336 None:intergenic
CTCCAAGAAGATAGCAATAA+TGG 0.269830 1.4:-70486530 MS.gene07196:CDS
TCAAAACTTGTTCGGGAATT+GGG 0.278093 1.4:-70486463 MS.gene07196:CDS
ATCAAAACTTGTTCGGGAAT+TGG 0.285433 1.4:-70486464 MS.gene07196:CDS
GATGGAGATTTAAAGCTCTT+TGG 0.296201 1.4:-70486947 MS.gene07196:intron
TCGTGATTCAAACAATTTCT+TGG 0.300130 1.4:+70486255 None:intergenic
GAAGAATGGAGAACACAAAA+AGG 0.349354 1.4:-70487147 MS.gene07196:CDS
GGAGTCATTGCTTGTTAACT+TGG 0.352335 1.4:+70486549 None:intergenic
CGCATGGTGGCCGTTGAGTA+GGG 0.361457 1.4:+70487227 None:intergenic
AACAATGAGTATGCTGATAA+GGG 0.368137 1.4:-70486574 MS.gene07196:CDS
TTCTTTATGTTGCAGCCATT+TGG 0.379956 1.4:-70486989 MS.gene07196:intron
TAACAATGAGTATGCTGATA+AGG 0.387390 1.4:-70486575 MS.gene07196:CDS
AGTAGCATCAAAACTTGTTC+GGG 0.393847 1.4:-70486470 MS.gene07196:CDS
AAGTAGCATCAAAACTTGTT+CGG 0.398448 1.4:-70486471 MS.gene07196:CDS
GAGTTTATTTGGAATTCCTT+TGG 0.399412 1.4:-70486434 MS.gene07196:CDS
CCGCATGGTGGCCGTTGAGT+AGG 0.403862 1.4:+70487226 None:intergenic
TCCAAGAAGATAGCAATAAT+GGG 0.404293 1.4:-70486529 MS.gene07196:CDS
GTTAACATTAACTTGAAGAA+TGG 0.418899 1.4:-70487161 MS.gene07196:CDS
AGGTGACTCTTTCACCTTGG+TGG 0.445525 1.4:-70486323 MS.gene07196:CDS
AAATCTCCATCTTCAAAAGC+TGG 0.446504 1.4:+70486959 None:intergenic
ACTGTCTTAATTAGGTATCA+TGG 0.449391 1.4:+70486171 None:intergenic
GCTTACATTGAGTGAAATGA+AGG 0.454820 1.4:+70487121 None:intergenic
CACGATCTCATGTTAGTGCT+TGG 0.456189 1.4:-70486237 MS.gene07196:CDS
AAAATTGAAATGCAGAATCA+AGG 0.457301 1.4:-70486615 MS.gene07196:intron
AAAGCTGGAACTTGACCAAA+TGG 0.480424 1.4:+70486974 None:intergenic
ACGATCTCATGTTAGTGCTT+GGG 0.482947 1.4:-70486236 MS.gene07196:CDS
ATTGTCGGAGTCCAAATACT+TGG 0.498612 1.4:-70486347 MS.gene07196:CDS
ATGCTACTTGATCGAAGTGT+TGG 0.510802 1.4:+70486485 None:intergenic
GGGAGGTGACTCTTTCACCT+TGG 0.520363 1.4:-70486326 MS.gene07196:CDS
TGAAGAGAATGAAGCCAAGT+TGG 0.524273 1.4:-70486398 MS.gene07196:CDS
TTCATCAAGTAATGAAAAGT+AGG 0.527654 1.4:+70486286 None:intergenic
ATCAAGAATGCTATCCAACT+TGG 0.528639 1.4:+70486384 None:intergenic
CTACTCAACGGCCACCATGC+GGG 0.529590 1.4:-70487225 MS.gene07196:CDS
CAAAACTTGTTCGGGAATTG+GGG 0.535983 1.4:-70486462 MS.gene07196:CDS
TGTGTATGAGAAAAGATTGT+CGG 0.551593 1.4:-70486362 MS.gene07196:CDS
CTTAATTAGGTATCATGGCA+AGG 0.566951 1.4:+70486176 None:intergenic
AAATTGAAATGCAGAATCAA+GGG 0.575285 1.4:-70486614 MS.gene07196:intron
GCAAGGACTTTAGACCAAGC+TGG 0.580505 1.4:+70486193 None:intergenic
GCATGGTGGCCGTTGAGTAG+GGG 0.589594 1.4:+70487228 None:intergenic
ATATCACTGCAAGACCAGCT+TGG 0.601324 1.4:-70486207 MS.gene07196:CDS
ATCTCATGTTAGTGCTTGGG+TGG 0.606175 1.4:-70486233 MS.gene07196:CDS
CCAAGAAGATAGCAATAATG+GGG 0.612634 1.4:-70486528 MS.gene07196:CDS
CTTACATTGAGTGAAATGAA+GGG 0.613061 1.4:+70487122 None:intergenic
TTGTCGGAGTCCAAATACTT+GGG 0.617317 1.4:-70486346 MS.gene07196:CDS
GCACGGAAGCCCCTACTCAA+CGG 0.627204 1.4:-70487237 MS.gene07196:CDS
ACAATGAGTATGCTGATAAG+GGG 0.629847 1.4:-70486573 MS.gene07196:CDS
ATGGCATCAATAAAAGTGCA+CGG 0.642318 1.4:-70487254 MS.gene07196:CDS
CAGAGTTGCAGTAACCCGCA+TGG 0.653094 1.4:+70487211 None:intergenic
CCTACTCAACGGCCACCATG+CGG 0.660998 1.4:-70487226 MS.gene07196:CDS
TCGGAGTCCAAATACTTGGG+AGG 0.675284 1.4:-70486343 MS.gene07196:CDS
CAAGAAGATAGCAATAATGG+GGG 0.701769 1.4:-70486527 MS.gene07196:CDS
TAAATGATGCAAATCCACCA+AGG 0.702241 1.4:+70486309 None:intergenic
AGTTGCAGTAACCCGCATGG+TGG 0.707787 1.4:+70487214 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACATTTTCTACCAATATT+TGG - chr1.4:70486615-70486634 MS.gene07196:intron 20.0%
!!! TTTTCTTTTCATTTTTTGCA+AGG + chr1.4:70486590-70486609 None:intergenic 20.0%
! AAAATTGAAATGCAGAATCA+AGG - chr1.4:70486816-70486835 MS.gene07196:intron 25.0%
! AAATTGAAATGCAGAATCAA+GGG - chr1.4:70486817-70486836 MS.gene07196:intron 25.0%
! TTATGTCAAACACCAAATAT+TGG + chr1.4:70486630-70486649 None:intergenic 25.0%
! TTCATCAAGTAATGAAAAGT+AGG + chr1.4:70487148-70487167 None:intergenic 25.0%
!! GTTAACATTAACTTGAAGAA+TGG - chr1.4:70486270-70486289 MS.gene07196:CDS 25.0%
!! TCATTCTCTTCAACAATTTT+TGG + chr1.4:70487025-70487044 None:intergenic 25.0%
AACAATGAGTATGCTGATAA+GGG - chr1.4:70486857-70486876 MS.gene07196:intron 30.0%
ATTATACAGTTTGATCAACG+TGG + chr1.4:70486419-70486438 None:intergenic 30.0%
CTTACATTGAGTGAAATGAA+GGG + chr1.4:70486312-70486331 None:intergenic 30.0%
GACATAATATACAACACATG+TGG - chr1.4:70486643-70486662 MS.gene07196:intron 30.0%
TAACAATGAGTATGCTGATA+AGG - chr1.4:70486856-70486875 MS.gene07196:intron 30.0%
TCCAAGAAGATAGCAATAAT+GGG - chr1.4:70486902-70486921 MS.gene07196:intron 30.0%
TCGTGATTCAAACAATTTCT+TGG + chr1.4:70487179-70487198 None:intergenic 30.0%
TGTGTATGAGAAAAGATTGT+CGG - chr1.4:70487069-70487088 MS.gene07196:intron 30.0%
TTGGGGATAAAGAGTTTATT+TGG - chr1.4:70486986-70487005 MS.gene07196:intron 30.0%
! AAGTAGCATCAAAACTTGTT+CGG - chr1.4:70486960-70486979 MS.gene07196:CDS 30.0%
! GAGTTTATTTGGAATTCCTT+TGG - chr1.4:70486997-70487016 MS.gene07196:intron 30.0%
! TTTTTGCAAGGAGTGAAATT+AGG + chr1.4:70486578-70486597 None:intergenic 30.0%
!! TCAACAATTTTTGGATCCAA+AGG + chr1.4:70487016-70487035 None:intergenic 30.0%
AAATCTCCATCTTCAAAAGC+TGG + chr1.4:70486475-70486494 None:intergenic 35.0%
ACAATGAGTATGCTGATAAG+GGG - chr1.4:70486858-70486877 MS.gene07196:intron 35.0%
ATCAAGAATGCTATCCAACT+TGG + chr1.4:70487050-70487069 None:intergenic 35.0%
CAAGAAGATAGCAATAATGG+GGG - chr1.4:70486904-70486923 MS.gene07196:intron 35.0%
CCAAGAAGATAGCAATAATG+GGG - chr1.4:70486903-70486922 MS.gene07196:intron 35.0%
CTCCAAGAAGATAGCAATAA+TGG - chr1.4:70486901-70486920 MS.gene07196:intron 35.0%
GAAGAATGGAGAACACAAAA+AGG - chr1.4:70486284-70486303 MS.gene07196:CDS 35.0%
GCTTACATTGAGTGAAATGA+AGG + chr1.4:70486313-70486332 None:intergenic 35.0%
TAAATGATGCAAATCCACCA+AGG + chr1.4:70487125-70487144 None:intergenic 35.0%
TTCTTTATGTTGCAGCCATT+TGG - chr1.4:70486442-70486461 MS.gene07196:CDS 35.0%
! AGTAGCATCAAAACTTGTTC+GGG - chr1.4:70486961-70486980 MS.gene07196:CDS 35.0%
! ATCAAAACTTGTTCGGGAAT+TGG - chr1.4:70486967-70486986 MS.gene07196:CDS 35.0%
! ATGGCATCAATAAAAGTGCA+CGG - chr1.4:70486177-70486196 MS.gene07196:CDS 35.0%
! GATGGAGATTTAAAGCTCTT+TGG - chr1.4:70486484-70486503 MS.gene07196:CDS 35.0%
! TCAAAACTTGTTCGGGAATT+GGG - chr1.4:70486968-70486987 MS.gene07196:CDS 35.0%
AAAGCTGGAACTTGACCAAA+TGG + chr1.4:70486460-70486479 None:intergenic 40.0%
ACGATCTCATGTTAGTGCTT+GGG - chr1.4:70487195-70487214 MS.gene07196:CDS 40.0%
ATTGTCGGAGTCCAAATACT+TGG - chr1.4:70487084-70487103 MS.gene07196:intron 40.0%
CCCCATTATTGCTATCTTCT+TGG + chr1.4:70486906-70486925 None:intergenic 40.0%
TACAGTTTGATCAACGTGGT+TGG + chr1.4:70486415-70486434 None:intergenic 40.0%
TGAAGAGAATGAAGCCAAGT+TGG - chr1.4:70487033-70487052 MS.gene07196:intron 40.0%
TGTCATGTGCTACGTAAATC+AGG + chr1.4:70486525-70486544 None:intergenic 40.0%
TTGTCGGAGTCCAAATACTT+GGG - chr1.4:70487085-70487104 MS.gene07196:intron 40.0%
! AGATAGCAATAATGGGGGTA+TGG - chr1.4:70486909-70486928 MS.gene07196:intron 40.0%
! ATGCTACTTGATCGAAGTGT+TGG + chr1.4:70486949-70486968 None:intergenic 40.0%
! CAAAACTTGTTCGGGAATTG+GGG - chr1.4:70486969-70486988 MS.gene07196:CDS 40.0%
! GGAGTCATTGCTTGTTAACT+TGG + chr1.4:70486885-70486904 None:intergenic 40.0%
!! CAAGTTCCAGCTTTTGAAGA+TGG - chr1.4:70486466-70486485 MS.gene07196:CDS 40.0%
AGAGTCACCTCCCAAGTATT+TGG + chr1.4:70487098-70487117 None:intergenic 45.0%
ATATCACTGCAAGACCAGCT+TGG - chr1.4:70487224-70487243 MS.gene07196:CDS 45.0%
ATCTCATGTTAGTGCTTGGG+TGG - chr1.4:70487198-70487217 MS.gene07196:CDS 45.0%
CACGATCTCATGTTAGTGCT+TGG - chr1.4:70487194-70487213 MS.gene07196:CDS 45.0%
TCAACGTGGTTGGAACAAGA+AGG + chr1.4:70486405-70486424 None:intergenic 45.0%
AGGTGACTCTTTCACCTTGG+TGG - chr1.4:70487108-70487127 MS.gene07196:intron 50.0%
GCAAGGACTTTAGACCAAGC+TGG + chr1.4:70487241-70487260 None:intergenic 50.0%
TCGGAGTCCAAATACTTGGG+AGG - chr1.4:70487088-70487107 MS.gene07196:intron 50.0%
! AATGGGGGTATGGTCTGAAG+TGG - chr1.4:70486919-70486938 MS.gene07196:intron 50.0%
AGTTGCAGTAACCCGCATGG+TGG + chr1.4:70486220-70486239 None:intergenic 55.0%
CAGAGTTGCAGTAACCCGCA+TGG + chr1.4:70486223-70486242 None:intergenic 55.0%
GGGAGGTGACTCTTTCACCT+TGG - chr1.4:70487105-70487124 MS.gene07196:intron 55.0%
CCTACTCAACGGCCACCATG+CGG - chr1.4:70486205-70486224 MS.gene07196:CDS 60.0%
CGCATGGTGGCCGTTGAGTA+GGG + chr1.4:70486207-70486226 None:intergenic 60.0%
CTACTCAACGGCCACCATGC+GGG - chr1.4:70486206-70486225 MS.gene07196:CDS 60.0%
GCACGGAAGCCCCTACTCAA+CGG - chr1.4:70486194-70486213 MS.gene07196:CDS 60.0%
GCATGGTGGCCGTTGAGTAG+GGG + chr1.4:70486206-70486225 None:intergenic 60.0%
CCGCATGGTGGCCGTTGAGT+AGG + chr1.4:70486208-70486227 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 70486177 70487276 70486177 ID=MS.gene07196
chr1.4 mRNA 70486177 70487276 70486177 ID=MS.gene07196.t1;Parent=MS.gene07196
chr1.4 exon 70487127 70487276 70487127 ID=MS.gene07196.t1.exon1;Parent=MS.gene07196.t1
chr1.4 CDS 70487127 70487276 70487127 ID=cds.MS.gene07196.t1;Parent=MS.gene07196.t1
chr1.4 exon 70486948 70486996 70486948 ID=MS.gene07196.t1.exon2;Parent=MS.gene07196.t1
chr1.4 CDS 70486948 70486996 70486948 ID=cds.MS.gene07196.t1;Parent=MS.gene07196.t1
chr1.4 exon 70486177 70486622 70486177 ID=MS.gene07196.t1.exon3;Parent=MS.gene07196.t1
chr1.4 CDS 70486177 70486622 70486177 ID=cds.MS.gene07196.t1;Parent=MS.gene07196.t1
Gene Sequence

>MS.gene07196

ATGGCATCAATAAAAGTGCACGGAAGCCCCTACTCAACGGCCACCATGCGGGTTACTGCAACTCTGTATGAAAAACAGATTGAGTTTGAGTTTGTTAACATTAACTTGAAGAATGGAGAACACAAAAAGGAACCCTTCATTTCACTCAATGTAAGCAATATAAAAATATATATATATATATATATATCGTTCTGCTAGTTCACTTCAATTATGAATTGAAATTTTCCTTCTTGTTCCAACCACGTTGATCAAACTGTATAATTAATTCTTTATGTTGCAGCCATTTGGTCAAGTTCCAGCTTTTGAAGATGGAGATTTAAAGCTCTTTGGTAAAATTTATATGAACCTGATTTACGTAGCACATGACACTTTAGATTAAACATTAATTATGTGTATTTCCTAATTTCACTCCTTGCAAAAAATGAAAAGAAAAAAAAAAAACATTTTCTACCAATATTTGGTGTTTGACATAATATACAACACATGTGGTTATATTTAATTATTTTTATTTTCGTTTTTGAACAAGTTATTTTTATTTTCTTAAATTATCACTGATATCGATATTACAATGTATGTATAGTGTCTCTCTAATATTTGTGCGAGTGCTTCGTAGAATTTGATGTAGATATAAACTAATTAAAAATTGAAATGCAGAATCAAGGGCGATAACGCAATATATTAACAATGAGTATGCTGATAAGGGGACCAAGTTAACAAGCAATGACTCCAAGAAGATAGCAATAATGGGGGTATGGTCTGAAGTGGAGAGCCAACACTTCGATCAAGTAGCATCAAAACTTGTTCGGGAATTGGGGATAAAGAGTTTATTTGGAATTCCTTTGGATCCAAAAATTGTTGAAGAGAATGAAGCCAAGTTGGATAGCATTCTTGATGTGTATGAGAAAAGATTGTCGGAGTCCAAATACTTGGGAGGTGACTCTTTCACCTTGGTGGATTTGCATCATTTACCTACTTTTCATTACTTGATGAAGTCACAGACCAAGAAATTGTTTGAATCACGATCTCATGTTAGTGCTTGGGTGGCTGATATCACTGCAAGACCAGCTTGGTCTAAAGTCCTTGCCATGATACCTAATTAA

Protein sequence

>MS.gene07196.t1

MASIKVHGSPYSTATMRVTATLYEKQIEFEFVNINLKNGEHKKEPFISLNPFGQVPAFEDGDLKLFESRAITQYINNEYADKGTKLTSNDSKKIAIMGVWSEVESQHFDQVASKLVRELGIKSLFGIPLDPKIVEENEAKLDSILDVYEKRLSESKYLGGDSFTLVDLHHLPTFHYLMKSQTKKLFESRSHVSAWVADITARPAWSKVLAMIPN