Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07174.t1 | BAB70616.1 | 98.7 | 159 | 2 | 0 | 1 | 159 | 6 | 164 | 1.60E-82 | 315.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07174.t1 | P46423 | 65.4 | 159 | 55 | 0 | 1 | 159 | 5 | 163 | 9.5e-55 | 214.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07174.t1 | Q948X4 | 98.7 | 159 | 2 | 0 | 1 | 159 | 6 | 164 | 1.2e-82 | 315.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene07195 | MS.gene07174 | PPI |
| MS.gene07174 | MS.gene03034 | PPI |
| MS.gene07196 | MS.gene07174 | PPI |
| MS.gene07174 | MS.gene07192 | PPI |
| MS.gene07174 | MS.gene005619 | PPI |
| MS.gene07174 | MS.gene20712 | PPI |
| MS.gene005621 | MS.gene07174 | PPI |
| MS.gene07174 | MS.gene03036 | PPI |
| MS.gene07174 | MS.gene005620 | PPI |
| MS.gene03035 | MS.gene07174 | PPI |
| MS.gene07174 | MS.gene20713 | PPI |
| MS.gene20711 | MS.gene07174 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07174.t1 | MTR_1g088845 | 93.125 | 160 | 11 | 0 | 1 | 160 | 6 | 165 | 3.36e-109 | 311 |
| MS.gene07174.t1 | MTR_1g088840 | 88.125 | 160 | 19 | 0 | 1 | 160 | 6 | 165 | 1.81e-104 | 299 |
| MS.gene07174.t1 | MTR_1g088825 | 88.125 | 160 | 19 | 0 | 1 | 160 | 6 | 165 | 1.81e-104 | 299 |
| MS.gene07174.t1 | MTR_1g088840 | 88.034 | 117 | 14 | 0 | 44 | 160 | 2 | 118 | 1.31e-72 | 216 |
| MS.gene07174.t1 | MTR_3g064700 | 42.683 | 164 | 94 | 0 | 1 | 164 | 5 | 168 | 1.15e-42 | 142 |
| MS.gene07174.t1 | MTR_1g088850 | 38.788 | 165 | 100 | 1 | 1 | 164 | 5 | 169 | 9.97e-41 | 137 |
| MS.gene07174.t1 | MTR_1g026140 | 39.394 | 165 | 99 | 1 | 1 | 164 | 7 | 171 | 9.15e-40 | 135 |
| MS.gene07174.t1 | MTR_3g450790 | 37.870 | 169 | 105 | 0 | 4 | 172 | 7 | 175 | 2.02e-39 | 134 |
| MS.gene07174.t1 | MTR_3g450930 | 37.278 | 169 | 106 | 0 | 4 | 172 | 7 | 175 | 6.50e-38 | 130 |
| MS.gene07174.t1 | MTR_1g088840 | 85.507 | 69 | 10 | 0 | 92 | 160 | 1 | 69 | 7.06e-37 | 124 |
| MS.gene07174.t1 | MTR_5g090920 | 38.235 | 170 | 102 | 2 | 5 | 172 | 8 | 176 | 7.10e-37 | 127 |
| MS.gene07174.t1 | MTR_5g090910 | 36.970 | 165 | 103 | 1 | 5 | 168 | 8 | 172 | 3.69e-34 | 120 |
| MS.gene07174.t1 | MTR_5g090910 | 34.259 | 108 | 70 | 1 | 62 | 168 | 8 | 115 | 2.45e-14 | 67.4 |
| MS.gene07174.t1 | MTR_8g098420 | 27.500 | 160 | 111 | 2 | 22 | 180 | 26 | 181 | 5.62e-12 | 61.6 |
| MS.gene07174.t1 | MTR_8g098420 | 27.329 | 161 | 112 | 2 | 22 | 181 | 26 | 182 | 2.05e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07174.t1 | AT1G02920 | 60.759 | 158 | 62 | 0 | 3 | 160 | 8 | 165 | 9.28e-63 | 193 |
| MS.gene07174.t1 | AT2G02930 | 59.006 | 161 | 63 | 1 | 3 | 160 | 8 | 168 | 1.56e-62 | 192 |
| MS.gene07174.t1 | AT1G02930 | 59.494 | 158 | 63 | 1 | 3 | 160 | 8 | 164 | 1.73e-62 | 192 |
| MS.gene07174.t1 | AT1G02930 | 59.494 | 158 | 63 | 1 | 3 | 160 | 8 | 164 | 1.73e-62 | 192 |
| MS.gene07174.t1 | AT4G02520 | 57.764 | 161 | 65 | 1 | 3 | 160 | 8 | 168 | 9.75e-62 | 190 |
| MS.gene07174.t1 | AT2G47730 | 54.321 | 162 | 73 | 1 | 1 | 161 | 54 | 215 | 4.83e-60 | 188 |
| MS.gene07174.t1 | AT2G47730 | 54.321 | 162 | 73 | 1 | 1 | 161 | 54 | 215 | 4.83e-60 | 188 |
| MS.gene07174.t1 | AT1G02950 | 49.689 | 161 | 80 | 1 | 1 | 160 | 27 | 187 | 4.13e-56 | 177 |
| MS.gene07174.t1 | AT1G02950 | 49.689 | 161 | 80 | 1 | 1 | 160 | 27 | 187 | 4.13e-56 | 177 |
| MS.gene07174.t1 | AT1G02950 | 49.689 | 161 | 80 | 1 | 1 | 160 | 29 | 189 | 4.47e-56 | 177 |
| MS.gene07174.t1 | AT1G02950 | 49.689 | 161 | 80 | 1 | 1 | 160 | 39 | 199 | 6.75e-56 | 177 |
| MS.gene07174.t1 | AT1G02940 | 45.062 | 162 | 88 | 1 | 1 | 161 | 41 | 202 | 1.34e-45 | 150 |
| MS.gene07174.t1 | AT1G02940 | 45.062 | 162 | 88 | 1 | 1 | 161 | 41 | 202 | 1.34e-45 | 150 |
| MS.gene07174.t1 | AT1G02940 | 45.062 | 162 | 88 | 1 | 1 | 161 | 54 | 215 | 1.74e-45 | 150 |
| MS.gene07174.t1 | AT1G02940 | 45.062 | 162 | 88 | 1 | 1 | 161 | 66 | 227 | 2.06e-45 | 151 |
| MS.gene07174.t1 | AT3G62760 | 42.941 | 170 | 96 | 1 | 1 | 169 | 5 | 174 | 1.54e-44 | 147 |
| MS.gene07174.t1 | AT1G49860 | 44.785 | 163 | 89 | 1 | 19 | 180 | 25 | 187 | 5.16e-41 | 139 |
| MS.gene07174.t1 | AT3G03190 | 41.463 | 164 | 96 | 0 | 1 | 164 | 5 | 168 | 2.25e-37 | 128 |
| MS.gene07174.t1 | AT5G17220 | 37.288 | 177 | 111 | 0 | 1 | 177 | 5 | 181 | 1.22e-36 | 126 |
| MS.gene07174.t1 | AT2G30870 | 34.320 | 169 | 111 | 0 | 4 | 172 | 7 | 175 | 1.31e-34 | 121 |
| MS.gene07174.t1 | AT2G30860 | 34.524 | 168 | 110 | 0 | 5 | 172 | 8 | 175 | 6.45e-34 | 119 |
| MS.gene07174.t1 | AT2G30860 | 35.616 | 146 | 94 | 0 | 5 | 150 | 8 | 153 | 2.51e-29 | 106 |
| MS.gene07174.t1 | AT1G02950 | 43.137 | 102 | 57 | 1 | 60 | 160 | 60 | 161 | 1.72e-24 | 95.5 |
Find 45 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGGGATAAAGACTTTATT+TGG | 0.149009 | 1.4:+70735534 | MS.gene07174:CDS |
| ACTCATTATTGCCATCTTCT+TGG | 0.252577 | 1.4:-70735451 | None:intergenic |
| TCAAAACTTGTTTGGGAATT+GGG | 0.267427 | 1.4:+70735516 | MS.gene07174:CDS |
| ATCAAAACTTGTTTGGGAAT+TGG | 0.277142 | 1.4:+70735515 | MS.gene07174:CDS |
| GATGGAGATTTAAAGCTCTT+TGG | 0.296201 | 1.4:+70735173 | MS.gene07174:CDS |
| GACTTTATTTGGAATTCCTT+TGG | 0.310575 | 1.4:+70735545 | MS.gene07174:CDS |
| AAGTAGCATCAAAACTTGTT+TGG | 0.348556 | 1.4:+70735508 | MS.gene07174:CDS |
| GAGGAATGGTGAACACAAAA+AGG | 0.363061 | 1.4:+70734988 | MS.gene07174:CDS |
| AGTAGCATCAAAACTTGTTT+GGG | 0.371553 | 1.4:+70735509 | MS.gene07174:CDS |
| TTCTTTATGTTGCAGCCATT+TGG | 0.379956 | 1.4:+70735131 | MS.gene07174:intron |
| CCGCATGGTGGCCGTTGAGA+TGG | 0.384856 | 1.4:-70734909 | None:intergenic |
| GATCATGAGTATGCTGATAA+GGG | 0.387666 | 1.4:+70735405 | MS.gene07174:CDS |
| GGTGAGTGTTTCACCTTTGG+TGG | 0.391725 | 1.4:+70735657 | MS.gene07174:CDS |
| CGCATGGTGGCCGTTGAGAT+GGG | 0.394581 | 1.4:-70734908 | None:intergenic |
| TGATCATGAGTATGCTGATA+AGG | 0.403067 | 1.4:+70735404 | MS.gene07174:CDS |
| GGAGGTGAGTGTTTCACCTT+TGG | 0.413897 | 1.4:+70735654 | MS.gene07174:CDS |
| AAATCTCCATCTTCAAAAGC+TGG | 0.446504 | 1.4:-70735161 | None:intergenic |
| ATGCTACTTTATCGTAGTGT+AGG | 0.457201 | 1.4:-70735494 | None:intergenic |
| GCTTACATTGATTGATATGA+AGG | 0.465911 | 1.4:-70735014 | None:intergenic |
| AAAGCTGGAACTTGACCAAA+TGG | 0.480424 | 1.4:-70735146 | None:intergenic |
| TCCTTTGGATTCAAAAGTTG+TGG | 0.481662 | 1.4:+70735560 | MS.gene07174:CDS |
| CAAAACTTGTTTGGGAATTG+GGG | 0.490036 | 1.4:+70735517 | MS.gene07174:CDS |
| AATGAGTGTGTGGTCGGAAG+TGG | 0.508607 | 1.4:+70735467 | MS.gene07174:CDS |
| CATCTCAACGGCCACCATGC+GGG | 0.515678 | 1.4:+70734910 | MS.gene07174:CDS |
| ATTGTCTGAATCTAAATACA+TGG | 0.527540 | 1.4:+70735632 | MS.gene07174:CDS |
| ATCAAGAATGCTATCCAACT+TGG | 0.528639 | 1.4:-70735595 | None:intergenic |
| GGAAGAGAATGAAGCCAAGT+TGG | 0.538389 | 1.4:+70735581 | MS.gene07174:CDS |
| AAAATTGAAATGCAGAGTCA+AGG | 0.547213 | 1.4:+70735364 | MS.gene07174:intron |
| AAATTGAAATGCAGAGTCAA+GGG | 0.557402 | 1.4:+70735365 | MS.gene07174:intron |
| GTAAGCATTAACATGAGGAA+TGG | 0.557734 | 1.4:+70734974 | MS.gene07174:CDS |
| AAGCAGTGACTCCAAGAAGA+TGG | 0.558112 | 1.4:+70735440 | MS.gene07174:CDS |
| CTTACATTGATTGATATGAA+GGG | 0.578372 | 1.4:-70735013 | None:intergenic |
| TTCATCAAGTAGTGAAGAGA+AGG | 0.583253 | 1.4:-70735694 | None:intergenic |
| GGCAATAATGAGTGTGTGGT+CGG | 0.583453 | 1.4:+70735461 | MS.gene07174:CDS |
| GCATGGTGGCCGTTGAGATG+GGG | 0.584856 | 1.4:-70734907 | None:intergenic |
| GCACGGAAGCCCCATCTCAA+CGG | 0.594946 | 1.4:+70734898 | MS.gene07174:CDS |
| ATCATGAGTATGCTGATAAG+GGG | 0.611479 | 1.4:+70735406 | MS.gene07174:CDS |
| CCATCTCAACGGCCACCATG+CGG | 0.626175 | 1.4:+70734909 | MS.gene07174:CDS |
| AGTTTGTAAGCATTAACATG+AGG | 0.629431 | 1.4:+70734969 | MS.gene07174:CDS |
| TTGTCTGAATCTAAATACAT+GGG | 0.650116 | 1.4:+70735633 | MS.gene07174:CDS |
| CAGAGTTGCAGTAACCCGCA+TGG | 0.653094 | 1.4:-70734924 | None:intergenic |
| ACATGATGCAAATCCACCAA+AGG | 0.664596 | 1.4:-70735670 | None:intergenic |
| TCTGAATCTAAATACATGGG+AGG | 0.678031 | 1.4:+70735636 | MS.gene07174:CDS |
| AGTTGCAGTAACCCGCATGG+TGG | 0.707787 | 1.4:-70734921 | None:intergenic |
| AGATGGCAATAATGAGTGTG+TGG | 0.714038 | 1.4:+70735457 | MS.gene07174:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGTCTGAATCTAAATACA+TGG | + | chr1.4:70735632-70735651 | MS.gene07174:CDS | 25.0% |
| ! | TTAAAAACGTATTTGATGTC+TGG | + | chr1.4:70735241-70735260 | MS.gene07174:intron | 25.0% |
| ! | TTGTCTGAATCTAAATACAT+GGG | + | chr1.4:70735633-70735652 | MS.gene07174:CDS | 25.0% |
| !! | ATCAATTAGTTTGATCAATG+TGG | - | chr1.4:70735108-70735127 | None:intergenic | 25.0% |
| !! | CTTACATTGATTGATATGAA+GGG | - | chr1.4:70735016-70735035 | None:intergenic | 25.0% |
| AAAATTGAAATGCAGAGTCA+AGG | + | chr1.4:70735364-70735383 | MS.gene07174:intron | 30.0% | |
| AAATTGAAATGCAGAGTCAA+GGG | + | chr1.4:70735365-70735384 | MS.gene07174:intron | 30.0% | |
| AGTTTGTAAGCATTAACATG+AGG | + | chr1.4:70734969-70734988 | MS.gene07174:CDS | 30.0% | |
| TTGGGGATAAAGACTTTATT+TGG | + | chr1.4:70735534-70735553 | MS.gene07174:CDS | 30.0% | |
| ! | AAGTAGCATCAAAACTTGTT+TGG | + | chr1.4:70735508-70735527 | MS.gene07174:CDS | 30.0% |
| ! | AGTAGCATCAAAACTTGTTT+GGG | + | chr1.4:70735509-70735528 | MS.gene07174:CDS | 30.0% |
| ! | ATCAAAACTTGTTTGGGAAT+TGG | + | chr1.4:70735515-70735534 | MS.gene07174:CDS | 30.0% |
| ! | ATTAGTTTGATCAATGTGGT+TGG | - | chr1.4:70735104-70735123 | None:intergenic | 30.0% |
| ! | GACTTTATTTGGAATTCCTT+TGG | + | chr1.4:70735545-70735564 | MS.gene07174:CDS | 30.0% |
| ! | GCTTACATTGATTGATATGA+AGG | - | chr1.4:70735017-70735036 | None:intergenic | 30.0% |
| ! | TCAAAACTTGTTTGGGAATT+GGG | + | chr1.4:70735516-70735535 | MS.gene07174:CDS | 30.0% |
| AAATCTCCATCTTCAAAAGC+TGG | - | chr1.4:70735164-70735183 | None:intergenic | 35.0% | |
| ACTCATTATTGCCATCTTCT+TGG | - | chr1.4:70735454-70735473 | None:intergenic | 35.0% | |
| ATCAAGAATGCTATCCAACT+TGG | - | chr1.4:70735598-70735617 | None:intergenic | 35.0% | |
| ATCATGAGTATGCTGATAAG+GGG | + | chr1.4:70735406-70735425 | MS.gene07174:CDS | 35.0% | |
| GATCATGAGTATGCTGATAA+GGG | + | chr1.4:70735405-70735424 | MS.gene07174:CDS | 35.0% | |
| GTAAGCATTAACATGAGGAA+TGG | + | chr1.4:70734974-70734993 | MS.gene07174:CDS | 35.0% | |
| TCCTTTGGATTCAAAAGTTG+TGG | + | chr1.4:70735560-70735579 | MS.gene07174:CDS | 35.0% | |
| TCTGAATCTAAATACATGGG+AGG | + | chr1.4:70735636-70735655 | MS.gene07174:CDS | 35.0% | |
| TGATCATGAGTATGCTGATA+AGG | + | chr1.4:70735404-70735423 | MS.gene07174:CDS | 35.0% | |
| TTCATCAAGTAGTGAAGAGA+AGG | - | chr1.4:70735697-70735716 | None:intergenic | 35.0% | |
| TTCTTTATGTTGCAGCCATT+TGG | + | chr1.4:70735131-70735150 | MS.gene07174:intron | 35.0% | |
| ! | CAAAACTTGTTTGGGAATTG+GGG | + | chr1.4:70735517-70735536 | MS.gene07174:CDS | 35.0% |
| ! | GATGGAGATTTAAAGCTCTT+TGG | + | chr1.4:70735173-70735192 | MS.gene07174:CDS | 35.0% |
| ! | TCCACAACTTTTGAATCCAA+AGG | - | chr1.4:70735564-70735583 | None:intergenic | 35.0% |
| !! | ATGCTACTTTATCGTAGTGT+AGG | - | chr1.4:70735497-70735516 | None:intergenic | 35.0% |
| AAAGCTGGAACTTGACCAAA+TGG | - | chr1.4:70735149-70735168 | None:intergenic | 40.0% | |
| ACATGATGCAAATCCACCAA+AGG | - | chr1.4:70735673-70735692 | None:intergenic | 40.0% | |
| AGATGGCAATAATGAGTGTG+TGG | + | chr1.4:70735457-70735476 | MS.gene07174:CDS | 40.0% | |
| GAGGAATGGTGAACACAAAA+AGG | + | chr1.4:70734988-70735007 | MS.gene07174:CDS | 40.0% | |
| TCAATGTGGTTGGAACAAGA+AGG | - | chr1.4:70735094-70735113 | None:intergenic | 40.0% | |
| !! | CAAGTTCCAGCTTTTGAAGA+TGG | + | chr1.4:70735155-70735174 | MS.gene07174:CDS | 40.0% |
| AAGCAGTGACTCCAAGAAGA+TGG | + | chr1.4:70735440-70735459 | MS.gene07174:CDS | 45.0% | |
| GGAAGAGAATGAAGCCAAGT+TGG | + | chr1.4:70735581-70735600 | MS.gene07174:CDS | 45.0% | |
| GGCAATAATGAGTGTGTGGT+CGG | + | chr1.4:70735461-70735480 | MS.gene07174:CDS | 45.0% | |
| AATGAGTGTGTGGTCGGAAG+TGG | + | chr1.4:70735467-70735486 | MS.gene07174:CDS | 50.0% | |
| GGAGGTGAGTGTTTCACCTT+TGG | + | chr1.4:70735654-70735673 | MS.gene07174:CDS | 50.0% | |
| GGTGAGTGTTTCACCTTTGG+TGG | + | chr1.4:70735657-70735676 | MS.gene07174:CDS | 50.0% | |
| AGTTGCAGTAACCCGCATGG+TGG | - | chr1.4:70734924-70734943 | None:intergenic | 55.0% | |
| CAGAGTTGCAGTAACCCGCA+TGG | - | chr1.4:70734927-70734946 | None:intergenic | 55.0% | |
| CATCTCAACGGCCACCATGC+GGG | + | chr1.4:70734910-70734929 | MS.gene07174:CDS | 60.0% | |
| CCATCTCAACGGCCACCATG+CGG | + | chr1.4:70734909-70734928 | MS.gene07174:CDS | 60.0% | |
| CGCATGGTGGCCGTTGAGAT+GGG | - | chr1.4:70734911-70734930 | None:intergenic | 60.0% | |
| GCACGGAAGCCCCATCTCAA+CGG | + | chr1.4:70734898-70734917 | MS.gene07174:CDS | 60.0% | |
| GCATGGTGGCCGTTGAGATG+GGG | - | chr1.4:70734910-70734929 | None:intergenic | 60.0% | |
| CCGCATGGTGGCCGTTGAGA+TGG | - | chr1.4:70734912-70734931 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 70734896 | 70735740 | 70734896 | ID=MS.gene07174 |
| chr1.4 | mRNA | 70734896 | 70735740 | 70734896 | ID=MS.gene07174.t1;Parent=MS.gene07174 |
| chr1.4 | exon | 70734896 | 70735030 | 70734896 | ID=MS.gene07174.t1.exon1;Parent=MS.gene07174.t1 |
| chr1.4 | CDS | 70734896 | 70735030 | 70734896 | ID=cds.MS.gene07174.t1;Parent=MS.gene07174.t1 |
| chr1.4 | exon | 70735146 | 70735194 | 70735146 | ID=MS.gene07174.t1.exon2;Parent=MS.gene07174.t1 |
| chr1.4 | CDS | 70735146 | 70735194 | 70735146 | ID=cds.MS.gene07174.t1;Parent=MS.gene07174.t1 |
| chr1.4 | exon | 70735379 | 70735740 | 70735379 | ID=MS.gene07174.t1.exon3;Parent=MS.gene07174.t1 |
| chr1.4 | CDS | 70735379 | 70735740 | 70735379 | ID=cds.MS.gene07174.t1;Parent=MS.gene07174.t1 |
| Gene Sequence |
| Protein sequence |
