Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35221.t1 | AFK42475.1 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 5.50E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35221.t1 | Q9ZQX4 | 87.3 | 126 | 16 | 0 | 1 | 126 | 1 | 126 | 8.6e-58 | 224.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35221.t1 | I3SQD3 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 4.0e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052015 | MS.gene35221 | 0.813158 | 2.95E-51 | -1.69E-46 |
| MS.gene059103 | MS.gene35221 | 0.82068 | 6.04E-53 | -1.69E-46 |
| MS.gene059480 | MS.gene35221 | 0.819651 | 1.04E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene35221 | MS.gene007434 | PPI |
| MS.gene35221 | MS.gene22436 | PPI |
| MS.gene35221 | MS.gene007513 | PPI |
| MS.gene35221 | MS.gene029841 | PPI |
| MS.gene35221 | MS.gene45416 | PPI |
| MS.gene35221 | MS.gene24597 | PPI |
| MS.gene35221 | MS.gene20061 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35221.t1 | AT4G02620 | 87.302 | 126 | 16 | 0 | 1 | 126 | 1 | 126 | 4.46e-80 | 231 |
Find 28 sgRNAs with CRISPR-Local
Find 210 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCATAGTTGGGTTTCTTT+TGG | 0.252743 | 4.4:-18500156 | MS.gene35221:CDS |
| CTCATGATTCAGTTCTTTCA+CGG | 0.364488 | 4.4:-18498663 | MS.gene35221:CDS |
| TTTCCACAGGATACCATAGT+TGG | 0.389583 | 4.4:-18500167 | MS.gene35221:intron |
| GAAGGGATTTCAAGAATTGC+AGG | 0.394210 | 4.4:+18498709 | None:intergenic |
| ATTTCAAGAATTGCAGGAAC+TGG | 0.410123 | 4.4:+18498715 | None:intergenic |
| GAATCATGAGCAGGATCATA+AGG | 0.423283 | 4.4:+18498676 | None:intergenic |
| AGCCAAAAGAAACCCAACTA+TGG | 0.431491 | 4.4:+18500154 | None:intergenic |
| ACTTACTTGAATCCACAATA+AGG | 0.438291 | 4.4:+18500093 | None:intergenic |
| TGCAGGTTGCAAATATGATA+AGG | 0.450588 | 4.4:-18498762 | MS.gene35221:intron |
| GATGAATTGTTGGCAGGAAT+CGG | 0.453548 | 4.4:+18501431 | None:intergenic |
| GGCAATAAGTGATGAATTGT+TGG | 0.454916 | 4.4:+18501421 | None:intergenic |
| GCTTACCTCGTCAGCAATCA+TGG | 0.470639 | 4.4:+18501400 | None:intergenic |
| GAAGACAAATTACCTTATTG+TGG | 0.485357 | 4.4:-18500105 | MS.gene35221:CDS |
| TTCCACAGGATACCATAGTT+GGG | 0.495606 | 4.4:-18500166 | MS.gene35221:intron |
| TTCAATGCTGAATCAGTGGC+AGG | 0.511114 | 4.4:-18498628 | MS.gene35221:CDS |
| TCATGATTCAGTTCTTTCAC+GGG | 0.514197 | 4.4:-18498662 | MS.gene35221:CDS |
| GGCAGGAATCGGAACTCTGT+TGG | 0.544048 | 4.4:+18501442 | None:intergenic |
| ATAAGTGATGAATTGTTGGC+AGG | 0.546049 | 4.4:+18501425 | None:intergenic |
| AATCATGAGCAGGATCATAA+GGG | 0.560484 | 4.4:+18498677 | None:intergenic |
| GAAAGAACTGAATCATGAGC+AGG | 0.572900 | 4.4:+18498667 | None:intergenic |
| TATTGCCATGATTGCTGACG+AGG | 0.587291 | 4.4:-18501405 | MS.gene35221:intron |
| TCTCTTCAATGCTGAATCAG+TGG | 0.629677 | 4.4:-18498632 | MS.gene35221:CDS |
| AACGACTGTCAAACAAATCG+AGG | 0.646091 | 4.4:-18499464 | MS.gene35221:CDS |
| CAAAGAATTCTCAACACGGG+AGG | 0.652854 | 4.4:-18499434 | MS.gene35221:CDS |
| ATTCAAAGAATTCTCAACAC+GGG | 0.675761 | 4.4:-18499437 | MS.gene35221:CDS |
| CATTCAAAGAATTCTCAACA+CGG | 0.678390 | 4.4:-18499438 | MS.gene35221:CDS |
| CATGAGCAGGATCATAAGGG+TGG | 0.700765 | 4.4:+18498680 | None:intergenic |
| TGGGAAACGTTGACATACGT+CGG | 0.705364 | 4.4:-18500127 | MS.gene35221:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTAGTTATTCAAATTAATA+GGG | + | chr4.4:18499717-18499736 | None:intergenic | 15.0% |
| !! | GAAAACTCAATTAATAAAAA+AGG | + | chr4.4:18499114-18499133 | None:intergenic | 15.0% |
| !! | TATGAAATGTATTAAAGATA+AGG | + | chr4.4:18500266-18500285 | None:intergenic | 15.0% |
| !! | TTACTTACTATTATTTATGA+GGG | - | chr4.4:18500004-18500023 | MS.gene35221:intron | 15.0% |
| !! | TTTACTTACTATTATTTATG+AGG | - | chr4.4:18500003-18500022 | MS.gene35221:intron | 15.0% |
| !!! | AAAAAAATCTAAAATGGAAT+TGG | + | chr4.4:18499799-18499818 | None:intergenic | 15.0% |
| !!! | AAAAAATCTAAAATGGAATT+GGG | + | chr4.4:18499798-18499817 | None:intergenic | 15.0% |
| !! | AAATACCAGCTTATATAATA+TGG | + | chr4.4:18499332-18499351 | None:intergenic | 20.0% |
| !! | AACATGTGTTAAACTATAAT+AGG | + | chr4.4:18500835-18500854 | None:intergenic | 20.0% |
| !! | AATTTGTAAGTCATATTTCA+TGG | - | chr4.4:18500674-18500693 | MS.gene35221:intron | 20.0% |
| !! | ATGAAATATGACTTACAAAT+TGG | + | chr4.4:18500675-18500694 | None:intergenic | 20.0% |
| !! | ATTAACAGGAAAAAATTGTA+TGG | + | chr4.4:18500046-18500065 | None:intergenic | 20.0% |
| !! | CACTAGTTATTCAAATTAAT+AGG | + | chr4.4:18499718-18499737 | None:intergenic | 20.0% |
| !! | CTAGTTATTCAAATTAATAG+GGG | + | chr4.4:18499716-18499735 | None:intergenic | 20.0% |
| !! | TAACAGGATTTAAATGAAAT+AGG | - | chr4.4:18499157-18499176 | MS.gene35221:intron | 20.0% |
| !! | TTAACAGGAAAAAATTGTAT+GGG | + | chr4.4:18500045-18500064 | None:intergenic | 20.0% |
| !! | TTAATAAAAAAGGAAATCAG+TGG | + | chr4.4:18499104-18499123 | None:intergenic | 20.0% |
| !!! | AAAATGTTGACTAGTTTATA+TGG | - | chr4.4:18501083-18501102 | MS.gene35221:intron | 20.0% |
| !!! | AACACATGTACTATTTTTAT+TGG | + | chr4.4:18500134-18500153 | None:intergenic | 20.0% |
| !!! | AATTCAATTTTATTTTCCAC+AGG | - | chr4.4:18499881-18499900 | MS.gene35221:intron | 20.0% |
| !!! | ATAAGAAAGTATATACAACA+TGG | + | chr4.4:18498693-18498712 | None:intergenic | 20.0% |
| !!! | TAAGAAAGTATATACAACAT+GGG | + | chr4.4:18498692-18498711 | None:intergenic | 20.0% |
| !!! | TGTTTTATTTCCTTATTTCA+GGG | + | chr4.4:18499484-18499503 | None:intergenic | 20.0% |
| !!! | TTAAAGATCTATTTTGCTAT+TGG | - | chr4.4:18498841-18498860 | MS.gene35221:intron | 20.0% |
| !!! | TTCAAAACAAATTGATTTGA+CGG | - | chr4.4:18500537-18500556 | MS.gene35221:intron | 20.0% |
| !!! | TTTTTGCTAGAATCTATTTA+AGG | + | chr4.4:18499136-18499155 | None:intergenic | 20.0% |
| ! | AAAATCAGATATAGTTCAGT+CGG | - | chr4.4:18499670-18499689 | MS.gene35221:intron | 25.0% |
| ! | AAATGAAATAGGAAATTGTG+AGG | - | chr4.4:18499168-18499187 | MS.gene35221:intron | 25.0% |
| ! | AATCAGAAAATTAGGTGAAA+AGG | + | chr4.4:18500100-18500119 | None:intergenic | 25.0% |
| ! | AATCAGATGATATGATAACT+AGG | - | chr4.4:18498741-18498760 | MS.gene35221:CDS | 25.0% |
| ! | AGATTCTAGCAAAAATTAAC+AGG | - | chr4.4:18499141-18499160 | MS.gene35221:intron | 25.0% |
| ! | ATACTCCTATTTCATTAACA+AGG | + | chr4.4:18499373-18499392 | None:intergenic | 25.0% |
| ! | CTCTGATAAAATTGAAGAAA+GGG | - | chr4.4:18499222-18499241 | MS.gene35221:intron | 25.0% |
| ! | GTGAGATTCAATCATAAAAT+TGG | - | chr4.4:18501002-18501021 | MS.gene35221:intron | 25.0% |
| ! | GTGTTAAACTATAATAGGTA+AGG | + | chr4.4:18500830-18500849 | None:intergenic | 25.0% |
| ! | TAAATAACCCAAGATTGTAA+CGG | + | chr4.4:18501257-18501276 | None:intergenic | 25.0% |
| ! | TAACAGGAAAAAATTGTATG+GGG | + | chr4.4:18500044-18500063 | None:intergenic | 25.0% |
| ! | TACTTCCATATTATATAAGC+TGG | - | chr4.4:18499324-18499343 | MS.gene35221:intron | 25.0% |
| ! | TAGCCAATTACTAGTATAAA+CGG | - | chr4.4:18499456-18499475 | MS.gene35221:CDS | 25.0% |
| ! | TATAATTTCTTTGACTATGC+AGG | - | chr4.4:18501282-18501301 | MS.gene35221:intron | 25.0% |
| ! | TCAGTTATTAGTTGGATATT+TGG | - | chr4.4:18501128-18501147 | MS.gene35221:intron | 25.0% |
| ! | TCTCTGATAAAATTGAAGAA+AGG | - | chr4.4:18499221-18499240 | MS.gene35221:intron | 25.0% |
| ! | TGTATTAAAGATAAGGTTTG+TGG | + | chr4.4:18500259-18500278 | None:intergenic | 25.0% |
| ! | TTTATGCAAAGGTTATTAGT+AGG | - | chr4.4:18500342-18500361 | MS.gene35221:intron | 25.0% |
| !! | AAACCGTAGAATACTTATTT+TGG | - | chr4.4:18500305-18500324 | MS.gene35221:intron | 25.0% |
| !! | AAATTTAGTTTCTTTGGATG+AGG | + | chr4.4:18499198-18499217 | None:intergenic | 25.0% |
| !! | ACTATTATTGTACATTTTCC+TGG | - | chr4.4:18498908-18498927 | MS.gene35221:intron | 25.0% |
| !! | AGAGACAAATTTAGTTTCTT+TGG | + | chr4.4:18499204-18499223 | None:intergenic | 25.0% |
| !! | AGGATAAGTAAAAAGTCTTT+TGG | + | chr4.4:18500810-18500829 | None:intergenic | 25.0% |
| !! | ATTTTATGATTGAATCTCAC+TGG | + | chr4.4:18501002-18501021 | None:intergenic | 25.0% |
| !! | TAGATTTGACTTAGAGTAAT+TGG | + | chr4.4:18499438-18499457 | None:intergenic | 25.0% |
| !! | TATTAGCTAAGATGATTTTG+CGG | + | chr4.4:18498790-18498809 | None:intergenic | 25.0% |
| !! | TGCAAATATGATAAGGTTTT+TGG | - | chr4.4:18501306-18501325 | MS.gene35221:intron | 25.0% |
| !!! | AACCGTAGAATACTTATTTT+GGG | - | chr4.4:18500306-18500325 | MS.gene35221:intron | 25.0% |
| !!! | GTGTTTTATTTCCTTATTTC+AGG | + | chr4.4:18499485-18499504 | None:intergenic | 25.0% |
| !!! | TTTTAATTATCTACTGCATC+AGG | - | chr4.4:18500901-18500920 | MS.gene35221:intron | 25.0% |
| AAAATCCATACAATGACAGT+TGG | - | chr4.4:18499521-18499540 | MS.gene35221:intron | 30.0% | |
| AACAGGAAAAAATTGTATGG+GGG | + | chr4.4:18500043-18500062 | None:intergenic | 30.0% | |
| AATGAATAATGCTGGATAAC+TGG | - | chr4.4:18500945-18500964 | MS.gene35221:intron | 30.0% | |
| ACTTACTTGAATCCACAATA+AGG | + | chr4.4:18499971-18499990 | None:intergenic | 30.0% | |
| AGAAAACCTTCTTGCTTATT+TGG | - | chr4.4:18501231-18501250 | MS.gene35221:intron | 30.0% | |
| AGTTTAACACATGTTCTGTT+TGG | - | chr4.4:18500840-18500859 | MS.gene35221:intron | 30.0% | |
| ATGAGGTTAATGAATAATGC+TGG | - | chr4.4:18500937-18500956 | MS.gene35221:intron | 30.0% | |
| ATTCAAAGAATTCTCAACAC+GGG | - | chr4.4:18500624-18500643 | MS.gene35221:intron | 30.0% | |
| CATTCAAAGAATTCTCAACA+CGG | - | chr4.4:18500623-18500642 | MS.gene35221:intron | 30.0% | |
| CATTTCTACACATCCTATAT+AGG | + | chr4.4:18500227-18500246 | None:intergenic | 30.0% | |
| GAAGACAAATTACCTTATTG+TGG | - | chr4.4:18499956-18499975 | MS.gene35221:intron | 30.0% | |
| GGTAATCAACACTATTGTTA+GGG | - | chr4.4:18500861-18500880 | MS.gene35221:intron | 30.0% | |
| GTTCCGTTTATACTAGTAAT+TGG | + | chr4.4:18499462-18499481 | None:intergenic | 30.0% | |
| TAAAAACTATTTCATGCACC+CGG | - | chr4.4:18501199-18501218 | MS.gene35221:intron | 30.0% | |
| TAGAAAATAAGTGGATGGAT+AGG | - | chr4.4:18499003-18499022 | MS.gene35221:intron | 30.0% | |
| TCTACCACAATCAGAAAATT+AGG | + | chr4.4:18500108-18500127 | None:intergenic | 30.0% | |
| TCTTGAAAGGATCAAATTCA+GGG | - | chr4.4:18498978-18498997 | MS.gene35221:intron | 30.0% | |
| TGCCCAAAATAAGTATTCTA+CGG | + | chr4.4:18500311-18500330 | None:intergenic | 30.0% | |
| TGGTAATCAACACTATTGTT+AGG | - | chr4.4:18500860-18500879 | MS.gene35221:intron | 30.0% | |
| TTAATTGGTACTTGAACCTT+GGG | - | chr4.4:18500381-18500400 | MS.gene35221:intron | 30.0% | |
| TTCAGGGTTTAGAAAATAAG+TGG | - | chr4.4:18498994-18499013 | MS.gene35221:intron | 30.0% | |
| ! | AAAAGGAAATCAGTGGTTTT+AGG | + | chr4.4:18499097-18499116 | None:intergenic | 30.0% |
| ! | AACTGTCATTGTATGGATTT+TGG | + | chr4.4:18499522-18499541 | None:intergenic | 30.0% |
| ! | AAGGTTTTCTAAATAGGAAC+CGG | + | chr4.4:18501221-18501240 | None:intergenic | 30.0% |
| ! | AAGTATTCTACGGTTTTTCT+GGG | + | chr4.4:18500301-18500320 | None:intergenic | 30.0% |
| ! | AGCAAGAAGGTTTTCTAAAT+AGG | + | chr4.4:18501227-18501246 | None:intergenic | 30.0% |
| ! | ATAGTTTTTAAATCCCAGCT+AGG | + | chr4.4:18501189-18501208 | None:intergenic | 30.0% |
| ! | CATTAACCTCATGTAAGTTT+TGG | + | chr4.4:18500929-18500948 | None:intergenic | 30.0% |
| ! | GATTGCCTTGTTAATGAAAT+AGG | - | chr4.4:18499365-18499384 | MS.gene35221:intron | 30.0% |
| ! | TAAGTATTCTACGGTTTTTC+TGG | + | chr4.4:18500302-18500321 | None:intergenic | 30.0% |
| ! | TTCACCTAATTTTCTGATTG+TGG | - | chr4.4:18500101-18500120 | MS.gene35221:CDS | 30.0% |
| !! | AACAGCCTGAATCATTTTTA+TGG | + | chr4.4:18500700-18500719 | None:intergenic | 30.0% |
| !! | TCAAGTTCTGAAAATATTCC+TGG | - | chr4.4:18500498-18500517 | MS.gene35221:intron | 30.0% |
| !! | TGACTAGTTTATATGGTCAT+TGG | - | chr4.4:18501090-18501109 | MS.gene35221:intron | 30.0% |
| !!! | AGAATACTTATTTTGGGCAT+GGG | - | chr4.4:18500312-18500331 | MS.gene35221:intron | 30.0% |
| !!! | ATTGTATGGATTTTGGCTTT+GGG | + | chr4.4:18499515-18499534 | None:intergenic | 30.0% |
| !!! | TAGAATACTTATTTTGGGCA+TGG | - | chr4.4:18500311-18500330 | MS.gene35221:intron | 30.0% |
| !!! | TGGGCTATTTTTTTATGCAA+AGG | - | chr4.4:18500331-18500350 | MS.gene35221:intron | 30.0% |
| AAACTGAGCAAGAAATTACC+AGG | + | chr4.4:18500519-18500538 | None:intergenic | 35.0% | |
| AACATGATGCACAGATTAAC+AGG | + | chr4.4:18500060-18500079 | None:intergenic | 35.0% | |
| AATCATGAGCAGGATCATAA+GGG | + | chr4.4:18501387-18501406 | None:intergenic | 35.0% | |
| ACCAATTAACCTCAGTTTCA+GGG | + | chr4.4:18500370-18500389 | None:intergenic | 35.0% | |
| ACTAGGATGCATGATTGAAT+AGG | - | chr4.4:18498758-18498777 | MS.gene35221:CDS | 35.0% | |
| AGAAAATTAGGTGAAAAGGC+AGG | + | chr4.4:18500096-18500115 | None:intergenic | 35.0% | |
| AGACTTACTGTTGTATATCG+TGG | - | chr4.4:18500170-18500189 | MS.gene35221:intron | 35.0% | |
| ATAAACGGAACCCTGAAATA+AGG | - | chr4.4:18499471-18499490 | MS.gene35221:intron | 35.0% | |
| ATAAGTGATGAATTGTTGGC+AGG | + | chr4.4:18498639-18498658 | None:intergenic | 35.0% | |
| ATTGTTAGGGAAAATCTGGA+GGG | - | chr4.4:18500874-18500893 | MS.gene35221:intron | 35.0% | |
| ATTTCAAGAATTGCAGGAAC+TGG | + | chr4.4:18501349-18501368 | None:intergenic | 35.0% | |
| CACTATTGTTAGGGAAAATC+TGG | - | chr4.4:18500870-18500889 | MS.gene35221:intron | 35.0% | |
| CATGGCCATAAAAATGATTC+AGG | - | chr4.4:18500692-18500711 | MS.gene35221:intron | 35.0% | |
| CTAGGATGCATGATTGAATA+GGG | - | chr4.4:18498759-18498778 | MS.gene35221:CDS | 35.0% | |
| CTCATGATTCAGTTCTTTCA+CGG | - | chr4.4:18501398-18501417 | MS.gene35221:intron | 35.0% | |
| CTCTTGAAAGGATCAAATTC+AGG | - | chr4.4:18498977-18498996 | MS.gene35221:intron | 35.0% | |
| CTGTTGGATCAGTTATTAGT+TGG | - | chr4.4:18501120-18501139 | MS.gene35221:intron | 35.0% | |
| GAAAATTAGGTGAAAAGGCA+GGG | + | chr4.4:18500095-18500114 | None:intergenic | 35.0% | |
| GAAAGGGATTTAGAGATCAT+AGG | - | chr4.4:18499238-18499257 | MS.gene35221:intron | 35.0% | |
| GGCAATAAGTGATGAATTGT+TGG | + | chr4.4:18498643-18498662 | None:intergenic | 35.0% | |
| GGGTTTAATAGGTTAGGTTT+AGG | - | chr4.4:18498952-18498971 | MS.gene35221:intron | 35.0% | |
| GGGTTTAGAAAATAAGTGGA+TGG | - | chr4.4:18498998-18499017 | MS.gene35221:intron | 35.0% | |
| GTTAATTGGTACTTGAACCT+TGG | - | chr4.4:18500380-18500399 | MS.gene35221:intron | 35.0% | |
| TACCAATTAACCTCAGTTTC+AGG | + | chr4.4:18500371-18500390 | None:intergenic | 35.0% | |
| TATTGTTAGGGAAAATCTGG+AGG | - | chr4.4:18500873-18500892 | MS.gene35221:intron | 35.0% | |
| TATTTGGCCGTTACAATCTT+GGG | - | chr4.4:18501247-18501266 | MS.gene35221:intron | 35.0% | |
| TCATGATTCAGTTCTTTCAC+GGG | - | chr4.4:18501399-18501418 | MS.gene35221:intron | 35.0% | |
| TGACTTCTCAAGTCATCTTA+TGG | + | chr4.4:18499051-18499070 | None:intergenic | 35.0% | |
| TGCAGGTTGCAAATATGATA+AGG | - | chr4.4:18501299-18501318 | MS.gene35221:intron | 35.0% | |
| TTATTTGGCCGTTACAATCT+TGG | - | chr4.4:18501246-18501265 | MS.gene35221:intron | 35.0% | |
| TTGTCGACATTTAAACCCAA+AGG | + | chr4.4:18501047-18501066 | None:intergenic | 35.0% | |
| ! | ACTTTCAGTTTTCAGCCATA+GGG | + | chr4.4:18499614-18499633 | None:intergenic | 35.0% |
| ! | AGGTTTTCTAAATAGGAACC+GGG | + | chr4.4:18501220-18501239 | None:intergenic | 35.0% |
| ! | AGTATTCTACGGTTTTTCTG+GGG | + | chr4.4:18500300-18500319 | None:intergenic | 35.0% |
| ! | TACCATAGTTGGGTTTCTTT+TGG | - | chr4.4:18499905-18499924 | MS.gene35221:intron | 35.0% |
| ! | TACTTTCAGTTTTCAGCCAT+AGG | + | chr4.4:18499615-18499634 | None:intergenic | 35.0% |
| ! | TAGGTTTAGGTTCTCTTGAA+AGG | - | chr4.4:18498965-18498984 | MS.gene35221:intron | 35.0% |
| ! | TATTGTACATTTTCCTGGTG+TGG | - | chr4.4:18498913-18498932 | MS.gene35221:intron | 35.0% |
| !! | TTTTCTTAAGAAGTGTGCAG+TGG | + | chr4.4:18498885-18498904 | None:intergenic | 35.0% |
| !!! | CATTGTATGGATTTTGGCTT+TGG | + | chr4.4:18499516-18499535 | None:intergenic | 35.0% |
| !!! | TTGTATGGATTTTGGCTTTG+GGG | + | chr4.4:18499514-18499533 | None:intergenic | 35.0% |
| AACGACTGTCAAACAAATCG+AGG | - | chr4.4:18500597-18500616 | MS.gene35221:intron | 40.0% | |
| AAGTAGGAACTAACAGTAGC+AGG | + | chr4.4:18500757-18500776 | None:intergenic | 40.0% | |
| ACACTCCAACTGTCATTGTA+TGG | + | chr4.4:18499529-18499548 | None:intergenic | 40.0% | |
| ACCGCACTAAAATATCCCTA+TGG | - | chr4.4:18499596-18499615 | MS.gene35221:intron | 40.0% | |
| ACGTAATAAGCTGATGTCTC+AGG | + | chr4.4:18500787-18500806 | None:intergenic | 40.0% | |
| ACTGCTTGGTACAAATCACA+GGG | + | chr4.4:18499401-18499420 | None:intergenic | 40.0% | |
| AGCCAAAAGAAACCCAACTA+TGG | + | chr4.4:18499910-18499929 | None:intergenic | 40.0% | |
| AGTAATCCAAGTACTACCCA+AGG | + | chr4.4:18500400-18500419 | None:intergenic | 40.0% | |
| ATCTGTGCATCATGTTCATG+TGG | - | chr4.4:18500064-18500083 | MS.gene35221:intron | 40.0% | |
| CAATGGCTGAATGAGCTAAA+AGG | + | chr4.4:18501160-18501179 | None:intergenic | 40.0% | |
| CAGGTTCCAAAACTTACATG+AGG | - | chr4.4:18500920-18500939 | MS.gene35221:intron | 40.0% | |
| CGACATTTAAACCCAAAGGA+TGG | + | chr4.4:18501043-18501062 | None:intergenic | 40.0% | |
| CGGCAATTGAACATTACAAC+TGG | - | chr4.4:18499690-18499709 | MS.gene35221:intron | 40.0% | |
| CTGTTAGTTCCTACTTCCTA+CGG | - | chr4.4:18500761-18500780 | MS.gene35221:intron | 40.0% | |
| GAAAGAACTGAATCATGAGC+AGG | + | chr4.4:18501397-18501416 | None:intergenic | 40.0% | |
| GAAGGGATTTCAAGAATTGC+AGG | + | chr4.4:18501355-18501374 | None:intergenic | 40.0% | |
| GAATCATGAGCAGGATCATA+AGG | + | chr4.4:18501388-18501407 | None:intergenic | 40.0% | |
| GATGAATTGTTGGCAGGAAT+CGG | + | chr4.4:18498633-18498652 | None:intergenic | 40.0% | |
| GATTGCATCACACATAGTCA+AGG | - | chr4.4:18499291-18499310 | MS.gene35221:intron | 40.0% | |
| GGGATATGTCTCTCCTATAT+AGG | - | chr4.4:18500211-18500230 | MS.gene35221:intron | 40.0% | |
| TAACGGCCAAATAAGCAAGA+AGG | + | chr4.4:18501240-18501259 | None:intergenic | 40.0% | |
| TACAATGACAGTTGGAGTGT+TGG | - | chr4.4:18499529-18499548 | MS.gene35221:intron | 40.0% | |
| TAGAAGGCTTGGGGTTTAAT+AGG | - | chr4.4:18498941-18498960 | MS.gene35221:intron | 40.0% | |
| TATCCCCATGCTTATTTACG+AGG | + | chr4.4:18500197-18500216 | None:intergenic | 40.0% | |
| TCTCTTCAATGCTGAATCAG+TGG | - | chr4.4:18501429-18501448 | MS.gene35221:CDS | 40.0% | |
| TCTTTCGATAGCCATCCTTT+GGG | - | chr4.4:18501029-18501048 | MS.gene35221:intron | 40.0% | |
| TGGACCTCGTAAATAAGCAT+GGG | - | chr4.4:18500190-18500209 | MS.gene35221:intron | 40.0% | |
| TTCCACAGGATACCATAGTT+GGG | - | chr4.4:18499895-18499914 | MS.gene35221:intron | 40.0% | |
| TTCTTTCGATAGCCATCCTT+TGG | - | chr4.4:18501028-18501047 | MS.gene35221:intron | 40.0% | |
| TTTCCACAGGATACCATAGT+TGG | - | chr4.4:18499894-18499913 | MS.gene35221:intron | 40.0% | |
| ! | ATAGTTGGGTTTCTTTTGGC+TGG | - | chr4.4:18499909-18499928 | MS.gene35221:intron | 40.0% |
| ! | CATAAGGGTGGTCTTTTGAA+GGG | + | chr4.4:18501372-18501391 | None:intergenic | 40.0% |
| ! | TCATAAGGGTGGTCTTTTGA+AGG | + | chr4.4:18501373-18501392 | None:intergenic | 40.0% |
| !!! | GCCATAGGGATATTTTAGTG+CGG | + | chr4.4:18499600-18499619 | None:intergenic | 40.0% |
| !!! | TGTATGGATTTTGGCTTTGG+GGG | + | chr4.4:18499513-18499532 | None:intergenic | 40.0% |
| AACCCAACTATGGTATCCTG+TGG | + | chr4.4:18499900-18499919 | None:intergenic | 45.0% | |
| AGCCATTGTGATATCCTAGC+TGG | - | chr4.4:18501172-18501191 | MS.gene35221:intron | 45.0% | |
| ATGGATAGGTTCTCGAGGAA+GGG | - | chr4.4:18499017-18499036 | MS.gene35221:intron | 45.0% | |
| CAAAGAATTCTCAACACGGG+AGG | - | chr4.4:18500627-18500646 | MS.gene35221:intron | 45.0% | |
| CTTGAACCTTGGGTAGTACT+TGG | - | chr4.4:18500391-18500410 | MS.gene35221:intron | 45.0% | |
| GACTGCTTGGTACAAATCAC+AGG | + | chr4.4:18499402-18499421 | None:intergenic | 45.0% | |
| GCCATTGTGATATCCTAGCT+GGG | - | chr4.4:18501173-18501192 | MS.gene35221:intron | 45.0% | |
| GCCCTGAAACTGAGGTTAAT+TGG | - | chr4.4:18500366-18500385 | MS.gene35221:intron | 45.0% | |
| GGAACTAACAGTAGCAGGTA+TGG | + | chr4.4:18500752-18500771 | None:intergenic | 45.0% | |
| GGACCTCGTAAATAAGCATG+GGG | - | chr4.4:18500191-18500210 | MS.gene35221:intron | 45.0% | |
| GGCTGAATGAGCTAAAAGGA+CGG | + | chr4.4:18501156-18501175 | None:intergenic | 45.0% | |
| GGCTTGGGGTTTAATAGGTT+AGG | - | chr4.4:18498946-18498965 | MS.gene35221:intron | 45.0% | |
| GTGGACCTCGTAAATAAGCA+TGG | - | chr4.4:18500189-18500208 | MS.gene35221:intron | 45.0% | |
| TATTGCCATGATTGCTGACG+AGG | - | chr4.4:18498656-18498675 | MS.gene35221:CDS | 45.0% | |
| TCCCAGCTAGGATATCACAA+TGG | + | chr4.4:18501177-18501196 | None:intergenic | 45.0% | |
| TCTCACTGGTACTGTCACAT+TGG | + | chr4.4:18500988-18501007 | None:intergenic | 45.0% | |
| TGCATCACACATAGTCAAGG+AGG | - | chr4.4:18499294-18499313 | MS.gene35221:intron | 45.0% | |
| TGGGAAACGTTGACATACGT+CGG | - | chr4.4:18499934-18499953 | MS.gene35221:intron | 45.0% | |
| TTCAATGCTGAATCAGTGGC+AGG | - | chr4.4:18501433-18501452 | MS.gene35221:CDS | 45.0% | |
| ! | TGTGGAGTACTAGAAGGCTT+GGG | - | chr4.4:18498931-18498950 | MS.gene35221:intron | 45.0% |
| !! | GGTCATTGGTTTCTCACTGT+TGG | - | chr4.4:18501104-18501123 | MS.gene35221:intron | 45.0% |
| !!! | TATTTTAGTGCGGTGCTGCT+AGG | + | chr4.4:18499590-18499609 | None:intergenic | 45.0% |
| !! | AAAAAAAAAAAAATCTAAAA+TGG | + | chr4.4:18499805-18499824 | None:intergenic | 5.0% |
| !!! | TTTTTTTTTTTTTTTGTAAT+TGG | - | chr4.4:18499816-18499835 | MS.gene35221:intron | 5.0% |
| ACCTGCAGTTTCAGACTGCT+TGG | + | chr4.4:18499415-18499434 | None:intergenic | 50.0% | |
| AGTGGATGGATAGGTTCTCG+AGG | - | chr4.4:18499012-18499031 | MS.gene35221:intron | 50.0% | |
| CATGAGCAGGATCATAAGGG+TGG | + | chr4.4:18501384-18501403 | None:intergenic | 50.0% | |
| GATGGATAGGTTCTCGAGGA+AGG | - | chr4.4:18499016-18499035 | MS.gene35221:intron | 50.0% | |
| GCTTACCTCGTCAGCAATCA+TGG | + | chr4.4:18498664-18498683 | None:intergenic | 50.0% | |
| TAGTAGGTGCCCTGAAACTG+AGG | - | chr4.4:18500358-18500377 | MS.gene35221:intron | 50.0% | |
| ! | ACCAAGCAGTCTGAAACTGC+AGG | - | chr4.4:18499411-18499430 | MS.gene35221:CDS | 50.0% |
| ! | GTGGAGTACTAGAAGGCTTG+GGG | - | chr4.4:18498932-18498951 | MS.gene35221:intron | 50.0% |
| ! | GTGTGGAGTACTAGAAGGCT+TGG | - | chr4.4:18498930-18498949 | MS.gene35221:intron | 50.0% |
| ! | TCCTGGTGTGGAGTACTAGA+AGG | - | chr4.4:18498925-18498944 | MS.gene35221:intron | 50.0% |
| !! | GGGTTTCTTTTGGCTGGAGT+GGG | - | chr4.4:18499915-18499934 | MS.gene35221:intron | 50.0% |
| !! | TGGGTTTCTTTTGGCTGGAG+TGG | - | chr4.4:18499914-18499933 | MS.gene35221:intron | 50.0% |
| AAGCTGATGTCTCAGGCCGT+AGG | + | chr4.4:18500780-18500799 | None:intergenic | 55.0% | |
| GCCTTCTAGTACTCCACACC+AGG | + | chr4.4:18498929-18498948 | None:intergenic | 55.0% | |
| GGCAGGAATCGGAACTCTGT+TGG | + | chr4.4:18498622-18498641 | None:intergenic | 55.0% | |
| TGTCTCAGGCCGTAGGAAGT+AGG | + | chr4.4:18500773-18500792 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 18498615 | 18501468 | 18498615 | ID=MS.gene35221 |
| chr4.4 | mRNA | 18498615 | 18501468 | 18498615 | ID=MS.gene35221.t1;Parent=MS.gene35221 |
| chr4.4 | exon | 18501406 | 18501468 | 18501406 | ID=MS.gene35221.t1.exon1;Parent=MS.gene35221.t1 |
| chr4.4 | CDS | 18501406 | 18501468 | 18501406 | ID=cds.MS.gene35221.t1;Parent=MS.gene35221.t1 |
| chr4.4 | exon | 18500099 | 18500180 | 18500099 | ID=MS.gene35221.t1.exon2;Parent=MS.gene35221.t1 |
| chr4.4 | CDS | 18500099 | 18500180 | 18500099 | ID=cds.MS.gene35221.t1;Parent=MS.gene35221.t1 |
| chr4.4 | exon | 18499405 | 18499487 | 18499405 | ID=MS.gene35221.t1.exon3;Parent=MS.gene35221.t1 |
| chr4.4 | CDS | 18499405 | 18499487 | 18499405 | ID=cds.MS.gene35221.t1;Parent=MS.gene35221.t1 |
| chr4.4 | exon | 18498615 | 18498779 | 18498615 | ID=MS.gene35221.t1.exon4;Parent=MS.gene35221.t1 |
| chr4.4 | CDS | 18498615 | 18498779 | 18498615 | ID=cds.MS.gene35221.t1;Parent=MS.gene35221.t1 |
| Gene Sequence |
| Protein sequence |
