Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014264.t1 | RHN71306.1 | 81.7 | 273 | 20 | 1 | 1 | 273 | 6 | 248 | 3.40E-124 | 454.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014264.t1 | Q9FMS6 | 59.5 | 269 | 104 | 3 | 8 | 271 | 9 | 277 | 2.4e-86 | 320.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014264.t1 | A0A396IZW4 | 81.7 | 273 | 20 | 1 | 1 | 273 | 6 | 248 | 2.5e-124 | 454.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene014264 | MS.gene21541 | PPI |
| MS.gene025629 | MS.gene014264 | PPI |
| MS.gene41100 | MS.gene014264 | PPI |
| MS.gene014264 | MS.gene41097 | PPI |
| MS.gene007154 | MS.gene014264 | PPI |
| MS.gene065707 | MS.gene014264 | PPI |
| MS.gene000609 | MS.gene014264 | PPI |
| MS.gene065708 | MS.gene014264 | PPI |
| MS.gene014264 | MS.gene025628 | PPI |
| MS.gene41101 | MS.gene014264 | PPI |
| MS.gene053225 | MS.gene014264 | PPI |
| MS.gene034250 | MS.gene014264 | PPI |
| MS.gene034816 | MS.gene014264 | PPI |
| MS.gene72977 | MS.gene014264 | PPI |
| MS.gene065709 | MS.gene014264 | PPI |
| MS.gene72978 | MS.gene014264 | PPI |
| MS.gene034815 | MS.gene014264 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014264.t1 | AT5G22250 | 58.333 | 276 | 110 | 3 | 1 | 271 | 2 | 277 | 1.84e-111 | 323 |
| MS.gene014264.t1 | AT3G44260 | 54.815 | 270 | 116 | 3 | 6 | 271 | 12 | 279 | 2.70e-102 | 300 |
| MS.gene014264.t1 | AT2G32070 | 46.241 | 266 | 137 | 5 | 9 | 272 | 8 | 269 | 9.73e-79 | 239 |
| MS.gene014264.t1 | AT5G10960 | 44.245 | 278 | 134 | 6 | 4 | 272 | 3 | 268 | 1.94e-76 | 234 |
| MS.gene014264.t1 | AT1G15920 | 44.118 | 272 | 143 | 5 | 5 | 270 | 8 | 276 | 5.80e-76 | 233 |
| MS.gene014264.t1 | AT1G15920 | 44.118 | 272 | 143 | 5 | 5 | 270 | 8 | 276 | 5.80e-76 | 233 |
| MS.gene014264.t1 | AT1G80780 | 43.774 | 265 | 144 | 5 | 9 | 272 | 8 | 268 | 1.03e-74 | 229 |
| MS.gene014264.t1 | AT1G80780 | 43.774 | 265 | 144 | 5 | 9 | 272 | 8 | 268 | 1.03e-74 | 229 |
| MS.gene014264.t1 | AT1G80780 | 43.774 | 265 | 144 | 5 | 9 | 272 | 8 | 268 | 1.31e-74 | 229 |
| MS.gene014264.t1 | AT1G61470 | 37.295 | 244 | 133 | 7 | 12 | 250 | 1 | 229 | 3.23e-38 | 135 |
| MS.gene014264.t1 | AT1G06450 | 33.969 | 262 | 154 | 8 | 12 | 269 | 8 | 254 | 4.53e-38 | 137 |
| MS.gene014264.t1 | AT3G44240 | 36.123 | 227 | 131 | 6 | 26 | 250 | 2 | 216 | 1.24e-35 | 128 |
| MS.gene014264.t1 | AT1G27820 | 35.685 | 241 | 137 | 7 | 15 | 250 | 8 | 235 | 1.65e-34 | 127 |
| MS.gene014264.t1 | AT1G27890 | 36.100 | 241 | 136 | 7 | 15 | 250 | 3 | 230 | 2.93e-34 | 126 |
Find 48 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATCATGATACGTGAAGTT+TGG | 0.309470 | 3.3:-95045431 | MS.gene014264:CDS |
| TGTCATCTGATGCGGGGATC+TGG | 0.360753 | 3.3:-95045033 | MS.gene014264:CDS |
| AATCTCATTCGTGGATTTGT+TGG | 0.372739 | 3.3:-95045384 | MS.gene014264:CDS |
| AACTGTATTGTTCGTAAGGT+TGG | 0.390597 | 3.3:+95045286 | None:intergenic |
| AACTATCAGATCCAGCTTGA+TGG | 0.414722 | 3.3:+95044764 | None:intergenic |
| CAAACTTCACGTATCATGAT+TGG | 0.415819 | 3.3:+95045432 | None:intergenic |
| ATCAATGGAATCTTTATTGT+GGG | 0.431524 | 3.3:+95045122 | None:intergenic |
| TAATATTCTCGATGTGGATC+GGG | 0.434798 | 3.3:-95044800 | MS.gene014264:CDS |
| TCGATGGATACTGAATTTCC+AGG | 0.435207 | 3.3:-95045345 | MS.gene014264:CDS |
| AATCATGATACGTGAAGTTT+GGG | 0.444962 | 3.3:-95045430 | MS.gene014264:CDS |
| AAATAACTGTATTGTTCGTA+AGG | 0.456687 | 3.3:+95045282 | None:intergenic |
| GCTCTTAACATCATTCAGAT+TGG | 0.459139 | 3.3:-95045243 | MS.gene014264:CDS |
| CTAATATTCTCGATGTGGAT+CGG | 0.460073 | 3.3:-95044801 | MS.gene014264:CDS |
| CTCGATGTGGATCGGGCCGT+TGG | 0.478690 | 3.3:-95044793 | MS.gene014264:CDS |
| TCACACCATGAGAGGCATTA+CGG | 0.480920 | 3.3:+95045070 | None:intergenic |
| ACTACACGGTGGTCTTGAAC+GGG | 0.485949 | 3.3:-95044827 | MS.gene014264:CDS |
| ATCATGATTGGTTCTGAACT+AGG | 0.486665 | 3.3:+95045444 | None:intergenic |
| GAGGAATTCAATCTCATTCG+TGG | 0.488675 | 3.3:-95045393 | MS.gene014264:CDS |
| AACATACAATTTCATCTCGA+TGG | 0.498778 | 3.3:-95045361 | MS.gene014264:CDS |
| CACTACACGGTGGTCTTGAA+CGG | 0.504975 | 3.3:-95044828 | MS.gene014264:CDS |
| ACTATCAGATCCAGCTTGAT+GGG | 0.512128 | 3.3:+95044765 | None:intergenic |
| TATCAATGGAATCTTTATTG+TGG | 0.521806 | 3.3:+95045121 | None:intergenic |
| GTTCGTAAGGTTGGAGATGA+CGG | 0.530754 | 3.3:+95045295 | None:intergenic |
| GTTGGAAAGTCCCATCAAGC+TGG | 0.534537 | 3.3:-95044775 | MS.gene014264:CDS |
| TTGACAGCGGAGCATATCAA+TGG | 0.541523 | 3.3:+95045107 | None:intergenic |
| ATTGATATGCTCCGCTGTCA+AGG | 0.556742 | 3.3:-95045105 | MS.gene014264:CDS |
| AAGTTATCTCCCGAATAGCT+TGG | 0.560061 | 3.3:-95044929 | MS.gene014264:CDS |
| TTCGTCCGTAATGCCTCTCA+TGG | 0.560305 | 3.3:-95045075 | MS.gene014264:CDS |
| CAATGAATTCACACCATGAG+AGG | 0.573117 | 3.3:+95045062 | None:intergenic |
| CAATGGAATCTTTATTGTGG+GGG | 0.584758 | 3.3:+95045124 | None:intergenic |
| CTCACCTTAACAGATGCAAA+AGG | 0.589044 | 3.3:-95045219 | MS.gene014264:CDS |
| CGCTACTGTAGTGCACTACA+CGG | 0.597011 | 3.3:-95044841 | MS.gene014264:CDS |
| TGACGGTGGTCAACTTTGAG+TGG | 0.600711 | 3.3:+95045312 | None:intergenic |
| TCGTCAACGAAGATGTGACA+TGG | 0.602644 | 3.3:-95045005 | MS.gene014264:CDS |
| TCAATGGAATCTTTATTGTG+GGG | 0.606892 | 3.3:+95045123 | None:intergenic |
| TTGAGTGGATAAATAACACC+TGG | 0.609070 | 3.3:+95045327 | None:intergenic |
| AGCTTGATGGGACTTTCCAA+CGG | 0.618452 | 3.3:+95044777 | None:intergenic |
| GAGACAAATCATTATGACGA+AGG | 0.645294 | 3.3:-95045472 | None:intergenic |
| GGTTGCTAATATTCTCGATG+TGG | 0.651359 | 3.3:-95044806 | MS.gene014264:CDS |
| GATCTGATAGTTTATTAACA+TGG | 0.652288 | 3.3:-95044753 | MS.gene014264:CDS |
| CGTGTAGTGCACTACAGTAG+CGG | 0.654385 | 3.3:+95044842 | None:intergenic |
| ATGTCACATCTTCGTTGACG+AGG | 0.659231 | 3.3:+95045007 | None:intergenic |
| CGTCAACGAAGATGTGACAT+GGG | 0.670296 | 3.3:-95045004 | MS.gene014264:CDS |
| GATGTTAAGAGCATCAACGT+TGG | 0.677679 | 3.3:+95045254 | None:intergenic |
| CGTAAGGTTGGAGATGACGG+TGG | 0.714506 | 3.3:+95045298 | None:intergenic |
| CGAAGTCAATACCTTGACAG+CGG | 0.743721 | 3.3:+95045094 | None:intergenic |
| TTGGGCACACAATCTGTACG+AGG | 0.755140 | 3.3:-95045412 | MS.gene014264:CDS |
| TACTGTAGTGCACTACACGG+TGG | 0.764520 | 3.3:-95044838 | MS.gene014264:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGTTTTGAGAAAATTATT+TGG | - | chr3.3:95045224-95045243 | MS.gene014264:CDS | 15.0% |
| ! | AAATAACTGTATTGTTCGTA+AGG | + | chr3.3:95044840-95044859 | None:intergenic | 25.0% |
| ! | ATCAATGGAATCTTTATTGT+GGG | + | chr3.3:95045000-95045019 | None:intergenic | 25.0% |
| ! | GATCTGATAGTTTATTAACA+TGG | - | chr3.3:95045366-95045385 | MS.gene014264:CDS | 25.0% |
| ! | TAAAAACATTCGTTGTATCT+GGG | - | chr3.3:95044938-95044957 | MS.gene014264:CDS | 25.0% |
| ! | TATCAATGGAATCTTTATTG+TGG | + | chr3.3:95045001-95045020 | None:intergenic | 25.0% |
| ! | TTAAAAACATTCGTTGTATC+TGG | - | chr3.3:95044937-95044956 | MS.gene014264:CDS | 25.0% |
| !! | AAAAAGTTTTCCAAGCTATT+CGG | + | chr3.3:95045203-95045222 | None:intergenic | 25.0% |
| !! | AAATATGCTGGAATCATTTT+CGG | - | chr3.3:95045440-95045459 | MS.gene014264:CDS | 25.0% |
| !! | AAATGATTCCAGCATATTTT+TGG | + | chr3.3:95045439-95045458 | None:intergenic | 25.0% |
| AACATACAATTTCATCTCGA+TGG | - | chr3.3:95044758-95044777 | MS.gene014264:CDS | 30.0% | |
| AATCATGATACGTGAAGTTT+GGG | - | chr3.3:95044689-95044708 | MS.gene014264:CDS | 30.0% | |
| AATGAAGTCCAAAAATATGC+TGG | - | chr3.3:95045428-95045447 | MS.gene014264:CDS | 30.0% | |
| TCAATGGAATCTTTATTGTG+GGG | + | chr3.3:95044999-95045018 | None:intergenic | 30.0% | |
| ! | AAAAGTTTTCCAAGCTATTC+GGG | + | chr3.3:95045202-95045221 | None:intergenic | 30.0% |
| !! | TGCATTTGATTTTGCATATC+TGG | - | chr3.3:95045151-95045170 | MS.gene014264:CDS | 30.0% |
| !!! | CAACGAATGTTTTTAAAACG+AGG | + | chr3.3:95044933-95044952 | None:intergenic | 30.0% |
| !!! | GAATGTTTTTAAAACGAGGT+AGG | + | chr3.3:95044929-95044948 | None:intergenic | 30.0% |
| AACTGTATTGTTCGTAAGGT+TGG | + | chr3.3:95044836-95044855 | None:intergenic | 35.0% | |
| AATCTCATTCGTGGATTTGT+TGG | - | chr3.3:95044735-95044754 | MS.gene014264:CDS | 35.0% | |
| CAAACTTCACGTATCATGAT+TGG | + | chr3.3:95044690-95044709 | None:intergenic | 35.0% | |
| CAATCATGATACGTGAAGTT+TGG | - | chr3.3:95044688-95044707 | MS.gene014264:CDS | 35.0% | |
| CAATGGAATCTTTATTGTGG+GGG | + | chr3.3:95044998-95045017 | None:intergenic | 35.0% | |
| CTAATATTCTCGATGTGGAT+CGG | - | chr3.3:95045318-95045337 | MS.gene014264:CDS | 35.0% | |
| GCTCTTAACATCATTCAGAT+TGG | - | chr3.3:95044876-95044895 | MS.gene014264:CDS | 35.0% | |
| TAATATTCTCGATGTGGATC+GGG | - | chr3.3:95045319-95045338 | MS.gene014264:CDS | 35.0% | |
| TTGAGTGGATAAATAACACC+TGG | + | chr3.3:95044795-95044814 | None:intergenic | 35.0% | |
| ! | ATGGCAGACTTTTCAGAAAA+TGG | - | chr3.3:95045385-95045404 | MS.gene014264:CDS | 35.0% |
| ! | GTTACCTTTTGCATCTGTTA+AGG | + | chr3.3:95044907-95044926 | None:intergenic | 35.0% |
| ! | TGCATTTTTGTCATCTGATG+CGG | - | chr3.3:95045078-95045097 | MS.gene014264:CDS | 35.0% |
| !! | ATCATGATTGGTTCTGAACT+AGG | + | chr3.3:95044678-95044697 | None:intergenic | 35.0% |
| !! | TGGAATCATTTTCGGATTAG+AGG | - | chr3.3:95045448-95045467 | MS.gene014264:CDS | 35.0% |
| AACTATCAGATCCAGCTTGA+TGG | + | chr3.3:95045358-95045377 | None:intergenic | 40.0% | |
| AAGTTATCTCCCGAATAGCT+TGG | - | chr3.3:95045190-95045209 | MS.gene014264:CDS | 40.0% | |
| ACTATCAGATCCAGCTTGAT+GGG | + | chr3.3:95045357-95045376 | None:intergenic | 40.0% | |
| CAATGAATTCACACCATGAG+AGG | + | chr3.3:95045060-95045079 | None:intergenic | 40.0% | |
| CTCACCTTAACAGATGCAAA+AGG | - | chr3.3:95044900-95044919 | MS.gene014264:CDS | 40.0% | |
| GAGGAATTCAATCTCATTCG+TGG | - | chr3.3:95044726-95044745 | MS.gene014264:CDS | 40.0% | |
| GGTTGCTAATATTCTCGATG+TGG | - | chr3.3:95045313-95045332 | MS.gene014264:CDS | 40.0% | |
| TCGATGGATACTGAATTTCC+AGG | - | chr3.3:95044774-95044793 | MS.gene014264:CDS | 40.0% | |
| ! | CATTTTTGTCATCTGATGCG+GGG | - | chr3.3:95045080-95045099 | MS.gene014264:CDS | 40.0% |
| ! | CTTTTGCATCTGTTAAGGTG+AGG | + | chr3.3:95044902-95044921 | None:intergenic | 40.0% |
| ! | GCATTTTTGTCATCTGATGC+GGG | - | chr3.3:95045079-95045098 | MS.gene014264:CDS | 40.0% |
| !! | GATGTTAAGAGCATCAACGT+TGG | + | chr3.3:95044868-95044887 | None:intergenic | 40.0% |
| AGCTTGATGGGACTTTCCAA+CGG | + | chr3.3:95045345-95045364 | None:intergenic | 45.0% | |
| ATGTCACATCTTCGTTGACG+AGG | + | chr3.3:95045115-95045134 | None:intergenic | 45.0% | |
| ATTGATATGCTCCGCTGTCA+AGG | - | chr3.3:95045014-95045033 | MS.gene014264:CDS | 45.0% | |
| CGAAGTCAATACCTTGACAG+CGG | + | chr3.3:95045028-95045047 | None:intergenic | 45.0% | |
| CGTCAACGAAGATGTGACAT+GGG | - | chr3.3:95045115-95045134 | MS.gene014264:CDS | 45.0% | |
| GTTCGTAAGGTTGGAGATGA+CGG | + | chr3.3:95044827-95044846 | None:intergenic | 45.0% | |
| TCGTCAACGAAGATGTGACA+TGG | - | chr3.3:95045114-95045133 | MS.gene014264:CDS | 45.0% | |
| TTGACAGCGGAGCATATCAA+TGG | + | chr3.3:95045015-95045034 | None:intergenic | 45.0% | |
| !! | TCACACCATGAGAGGCATTA+CGG | + | chr3.3:95045052-95045071 | None:intergenic | 45.0% |
| CGCTACTGTAGTGCACTACA+CGG | - | chr3.3:95045278-95045297 | MS.gene014264:CDS | 50.0% | |
| CGTGTAGTGCACTACAGTAG+CGG | + | chr3.3:95045280-95045299 | None:intergenic | 50.0% | |
| GTTGGAAAGTCCCATCAAGC+TGG | - | chr3.3:95045344-95045363 | MS.gene014264:CDS | 50.0% | |
| TACTGTAGTGCACTACACGG+TGG | - | chr3.3:95045281-95045300 | MS.gene014264:CDS | 50.0% | |
| TGACGGTGGTCAACTTTGAG+TGG | + | chr3.3:95044810-95044829 | None:intergenic | 50.0% | |
| TTCGTCCGTAATGCCTCTCA+TGG | - | chr3.3:95045044-95045063 | MS.gene014264:CDS | 50.0% | |
| TTGGGCACACAATCTGTACG+AGG | - | chr3.3:95044707-95044726 | MS.gene014264:CDS | 50.0% | |
| ! | ACTACACGGTGGTCTTGAAC+GGG | - | chr3.3:95045292-95045311 | MS.gene014264:CDS | 50.0% |
| ! | CACTACACGGTGGTCTTGAA+CGG | - | chr3.3:95045291-95045310 | MS.gene014264:CDS | 50.0% |
| TGTCATCTGATGCGGGGATC+TGG | - | chr3.3:95045086-95045105 | MS.gene014264:CDS | 55.0% | |
| ! | CGTAAGGTTGGAGATGACGG+TGG | + | chr3.3:95044824-95044843 | None:intergenic | 55.0% |
| CTCGATGTGGATCGGGCCGT+TGG | - | chr3.3:95045326-95045345 | MS.gene014264:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 95044660 | 95045481 | 95044660 | ID=MS.gene014264 |
| chr3.3 | mRNA | 95044660 | 95045481 | 95044660 | ID=MS.gene014264.t1;Parent=MS.gene014264 |
| chr3.3 | exon | 95044660 | 95045481 | 95044660 | ID=MS.gene014264.t1.exon1;Parent=MS.gene014264.t1 |
| chr3.3 | CDS | 95044660 | 95045481 | 95044660 | ID=cds.MS.gene014264.t1;Parent=MS.gene014264.t1 |
| Gene Sequence |
| Protein sequence |
