AlfalfaGEDB | MS.gene034816

M. sativa cultivar XinJiangDaYe / MS.gene034816

KEGG: K12581
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04857.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034816.t1	XP_024633363.1	95.8	288	8	1	1	284	1	288	4.60E-156	560.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034816.t1	Q9LXM2	61.2	276	95	4	10	281	11	278	1.1e-89	331.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034816.t1	A0A396JQM4	81.6	332	13	2	1	284	1	332	4.1e-146	526.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene034816	MS.gene85904	PPI
MS.gene053225	MS.gene034816	PPI
MS.gene034816	MS.gene030414	PPI
MS.gene034816	MS.gene84309	PPI
MS.gene53120	MS.gene034816	PPI
MS.gene72178	MS.gene034816	PPI
MS.gene034816	MS.gene034438	PPI
MS.gene68798	MS.gene034816	PPI
MS.gene35444	MS.gene034816	PPI
MS.gene034816	MS.gene85905	PPI
MS.gene034816	MS.gene53119	PPI
MS.gene034816	MS.gene000609	PPI
MS.gene034816	MS.gene55324	PPI
MS.gene034816	MS.gene020895	PPI
MS.gene034816	MS.gene38057	PPI
MS.gene034816	MS.gene72157	PPI
MS.gene81219	MS.gene034816	PPI
MS.gene034250	MS.gene034816	PPI
MS.gene034816	MS.gene85901	PPI
MS.gene034816	MS.gene78556	PPI
MS.gene034816	MS.gene85903	PPI
MS.gene034816	MS.gene35431	PPI
MS.gene38201	MS.gene034816	PPI
MS.gene034816	MS.gene38199	PPI
MS.gene53121	MS.gene034816	PPI
MS.gene034816	MS.gene72156	PPI
MS.gene034816	MS.gene53123	PPI
MS.gene030415	MS.gene034816	PPI
MS.gene034816	MS.gene048793	PPI
MS.gene68205	MS.gene034816	PPI
MS.gene034816	MS.gene048794	PPI
MS.gene034816	MS.gene014264	PPI
MS.gene034816	MS.gene35692	PPI
MS.gene034816	MS.gene53122	PPI
MS.gene070320	MS.gene034816	PPI
MS.gene78557	MS.gene034816	PPI
MS.gene72160	MS.gene034816	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034816.t1	AT3G44260	61.232	276	95	4	10	281	11	278	2.86e-114	330
MS.gene034816.t1	AT5G22250	61.993	271	92	5	16	281	12	276	1.64e-113	328
MS.gene034816.t1	AT2G32070	54.851	268	109	5	15	280	9	266	1.65e-97	288
MS.gene034816.t1	AT5G10960	55.805	267	107	5	15	280	9	265	4.47e-97	286
MS.gene034816.t1	AT1G80780	55.431	267	108	5	15	280	9	265	1.37e-94	280
MS.gene034816.t1	AT1G80780	55.431	267	108	5	15	280	9	265	1.37e-94	280
MS.gene034816.t1	AT1G80780	55.431	267	108	5	15	280	9	265	2.32e-94	280
MS.gene034816.t1	AT1G15920	50.000	270	128	5	15	282	13	277	5.92e-86	259
MS.gene034816.t1	AT1G15920	50.000	270	128	5	15	282	13	277	5.92e-86	259
MS.gene034816.t1	AT1G06450	34.082	267	149	8	19	280	10	254	2.60e-40	144
MS.gene034816.t1	AT1G61470	37.860	243	127	8	21	258	5	228	4.77e-39	138
MS.gene034816.t1	AT1G27820	37.052	251	132	9	15	258	3	234	4.07e-36	131
MS.gene034816.t1	AT1G27890	36.929	241	134	6	21	258	4	229	5.24e-32	120
MS.gene034816.t1	AT3G44240	36.245	229	128	6	32	258	3	215	6.51e-32	118

Find 0 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAAAAGGAGTTTCTAATTT+GGG	-	8685:224486-224505	MS.gene034816:CDS	25.0%
!	TAGAAAAGGAGTTTCTAATT+TGG	-	8685:224485-224504	MS.gene034816:CDS	25.0%
!!	AGAAACTCCTTTTCTAATTT+CGG	+	8685:224481-224500	None:intergenic	25.0%
	AAGATTAAGGAAGTTCACTT+TGG	-	8685:224949-224968	MS.gene034816:CDS	30.0%
	AGATTAAGGAAGTTCACTTT+GGG	-	8685:224950-224969	MS.gene034816:CDS	30.0%
	TAACCTTCCGAAATTAGAAA+AGG	-	8685:224471-224490	MS.gene034816:CDS	30.0%
	TCGAGAAGAATAAGGAATTT+GGG	-	8685:224593-224612	MS.gene034816:CDS	30.0%
	TTCGAGAAGAATAAGGAATT+TGG	-	8685:224592-224611	MS.gene034816:CDS	30.0%
!	GATTGATTTCGAGAAGAATA+AGG	-	8685:224585-224604	MS.gene034816:CDS	30.0%
!	TGTTTATGATGTGAAGCATT+TGG	-	8685:224801-224820	MS.gene034816:CDS	30.0%
!!	ATTTGGTGAAGTTTTGTGAA+GGG	-	8685:224818-224837	MS.gene034816:CDS	30.0%
!!	GTTGTATGGATTAGAAAATG+TGG	-	8685:225008-225027	MS.gene034816:CDS	30.0%
!!	TCATGCTTTTAGGAAGATTA+AGG	-	8685:224936-224955	MS.gene034816:CDS	30.0%
!!	TTTCATAGCGGATATGATTT+CGG	-	8685:224691-224710	MS.gene034816:CDS	30.0%
	AATTGAATTGCTGAAGAGTC+AGG	-	8685:224561-224580	MS.gene034816:CDS	35.0%
	ATTGAATTGCTGAAGAGTCA+GGG	-	8685:224562-224581	MS.gene034816:CDS	35.0%
	CGGATATGATTTCGGATATT+TGG	-	8685:224699-224718	MS.gene034816:CDS	35.0%
	TCGATGGTAAGATGATAAGT+CGG	+	8685:224362-224381	None:intergenic	35.0%
!	ACTCCTTTTCTAATTTCGGA+AGG	+	8685:224477-224496	None:intergenic	35.0%
!	TGATTTCGGATATTTGGTGA+AGG	-	8685:224705-224724	MS.gene034816:CDS	35.0%
!!	AAGTTTTGTGAAGGGTTGTA+TGG	-	8685:224826-224845	MS.gene034816:CDS	35.0%
!!	CATTTGGTGAAGTTTTGTGA+AGG	-	8685:224817-224836	MS.gene034816:CDS	35.0%
	AAGTATGCTGGTGTGTTGTA+TGG	-	8685:224994-225013	MS.gene034816:CDS	40.0%
	ATTGAGATTGATGGAGCGTA+GGG	+	8685:224214-224233	None:intergenic	40.0%
	CTGATAAAGTGAGACCAACT+TGG	+	8685:224441-224460	None:intergenic	40.0%
	GATCGATTGATGAAGTATGC+TGG	-	8685:224982-225001	MS.gene034816:CDS	40.0%
	GATGTGAATCAACGAAAGAG+CGG	+	8685:224282-224301	None:intergenic	40.0%
	GGTCTTAATCTCATCCAAGT+TGG	-	8685:224424-224443	MS.gene034816:CDS	40.0%
	GTGTAATGAGGAAGTTAGCT+GGG	-	8685:224663-224682	MS.gene034816:CDS	40.0%
	TAATTTCGGAAGGTTACCCT+CGG	+	8685:224467-224486	None:intergenic	40.0%
	TGTGTAATGAGGAAGTTAGC+TGG	-	8685:224662-224681	MS.gene034816:CDS	40.0%
	TTCACATCCGATCATCTCAA+TGG	-	8685:224294-224313	MS.gene034816:CDS	40.0%
	TTCTGGACTTGTGTGTAATG+AGG	-	8685:224651-224670	MS.gene034816:CDS	40.0%
	TTGAATTGCTGAAGAGTCAG+GGG	-	8685:224563-224582	MS.gene034816:CDS	40.0%
!	AGGAATTTGGGATTGACTCA+TGG	-	8685:224605-224624	MS.gene034816:CDS	40.0%
!	ATGGTGGTTTGGATAGAGTT+GGG	-	8685:224845-224864	MS.gene034816:CDS	40.0%
!	TATGGTGGTTTGGATAGAGT+TGG	-	8685:224844-224863	MS.gene034816:CDS	40.0%
!	TTTTGTGAAGGGTTGTATGG+TGG	-	8685:224829-224848	MS.gene034816:CDS	40.0%
!!	GCTGAATTGATGATGAGTTC+TGG	-	8685:224634-224653	MS.gene034816:CDS	40.0%
!!	TGCTGGTTAGGGTTTTCTTT+GGG	-	8685:224773-224792	MS.gene034816:CDS	40.0%
!!!	TGCTGACTCTTCATGCTTTT+AGG	-	8685:224926-224945	MS.gene034816:CDS	40.0%
	AGAAACTCAGCAAGCTCATC+AGG	+	8685:224751-224770	None:intergenic	45.0%
	AGCTGGGTGACATTTCATAG+CGG	-	8685:224679-224698	MS.gene034816:CDS	45.0%
	CTCACTTTATCAGATGCCGA+GGG	-	8685:224448-224467	MS.gene034816:CDS	45.0%
	GATTGAGATTGATGGAGCGT+AGG	+	8685:224215-224234	None:intergenic	45.0%
	TCAATGGACACCGAATTTCC+CGG	-	8685:224310-224329	MS.gene034816:CDS	45.0%
	TCCATCAATCTCAATCCGAC+CGG	-	8685:224219-224238	MS.gene034816:CDS	45.0%
	TCGAGATTGAATGACCACAC+CGG	+	8685:224241-224260	None:intergenic	45.0%
	TCTCACTTTATCAGATGCCG+AGG	-	8685:224447-224466	MS.gene034816:CDS	45.0%
	TGAAGGGTTGTATGGTGGTT+TGG	-	8685:224834-224853	MS.gene034816:CDS	45.0%
!	GCTGGTTAGGGTTTTCTTTG+GGG	-	8685:224774-224793	MS.gene034816:CDS	45.0%
!	TCGGTGTCCATTGAGATGAT+CGG	+	8685:224304-224323	None:intergenic	45.0%
!	TGAATGTGGATCGTGTTGCT+GGG	-	8685:224875-224894	MS.gene034816:CDS	45.0%
!!	TGAGTTTCTGGTGCTGGTTA+GGG	-	8685:224762-224781	MS.gene034816:CDS	45.0%
!!	TGATGAGCTTGCTGAGTTTC+TGG	-	8685:224750-224769	MS.gene034816:CDS	45.0%
!!!	GTGCTGGTTAGGGTTTTCTT+TGG	-	8685:224772-224791	MS.gene034816:CDS	45.0%
	AATGAGTGGCAGGTGTTCGA+TGG	+	8685:224378-224397	None:intergenic	50.0%
	ACCGGTCGGATTGAGATTGA+TGG	+	8685:224223-224242	None:intergenic	50.0%
	AGTTGGGAAGACGCTGAATG+TGG	-	8685:224861-224880	MS.gene034816:CDS	50.0%
	ATAAGTCGGTGATGCCGTCA+GGG	+	8685:224348-224367	None:intergenic	50.0%
	CACTCATTACTCCGTCCTCA+AGG	-	8685:224390-224409	MS.gene034816:CDS	50.0%
	CTGAATGTGGATCGTGTTGC+TGG	-	8685:224874-224893	MS.gene034816:CDS	50.0%
	CTTGAGGACGGAGTAATGAG+TGG	+	8685:224392-224411	None:intergenic	50.0%
	GATTGAATGACCACACCGGT+CGG	+	8685:224237-224256	None:intergenic	50.0%
	TGTTGCTGGGAAGAGTCATC+AGG	-	8685:224888-224907	MS.gene034816:CDS	50.0%
!!	CTGAGTTTCTGGTGCTGGTT+AGG	-	8685:224761-224780	MS.gene034816:CDS	50.0%
	AGGACGGAGTAATGAGTGGC+AGG	+	8685:224388-224407	None:intergenic	55.0%
	CAATCTCAATCCGACCGGTG+TGG	-	8685:224224-224243	MS.gene034816:CDS	55.0%
	CTGGGAAGAGTCATCAGGCT+GGG	-	8685:224893-224912	MS.gene034816:CDS	55.0%
	GACGACAACGCCGGGAAATT+CGG	+	8685:224323-224342	None:intergenic	55.0%
	GATAAGTCGGTGATGCCGTC+AGG	+	8685:224349-224368	None:intergenic	55.0%
	GTCCTCAAGGCGAATGTCGA+CGG	-	8685:224403-224422	MS.gene034816:CDS	55.0%
	GTCGACATTCGCCTTGAGGA+CGG	+	8685:224404-224423	None:intergenic	55.0%
!!	GCTTGCTGAGTTTCTGGTGC+TGG	-	8685:224756-224775	MS.gene034816:CDS	55.0%
	GACCGTCGACATTCGCCTTG+AGG	+	8685:224408-224427	None:intergenic	60.0%
	GCTGGGAAGAGTCATCAGGC+TGG	-	8685:224892-224911	MS.gene034816:CDS	60.0%
	AGTCGGTGATGCCGTCAGGG+CGG	+	8685:224345-224364	None:intergenic	65.0%
	GGCGTTGTCGTCCGCCCTGA+CGG	-	8685:224331-224350	MS.gene034816:CDS	70.0%
	GTCAGGGCGGACGACAACGC+CGG	+	8685:224332-224351	None:intergenic	70.0%
	TCAGGGCGGACGACAACGCC+GGG	+	8685:224331-224350	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
8685	gene	224181	225035	224181	ID=MS.gene034816
8685	mRNA	224181	225035	224181	ID=MS.gene034816.t1;Parent=MS.gene034816
8685	exon	224181	225035	224181	ID=MS.gene034816.t1.exon1;Parent=MS.gene034816.t1
8685	CDS	224181	225035	224181	ID=cds.MS.gene034816.t1;Parent=MS.gene034816.t1

Gene Sequence

>MS.gene034816

ATGTTCACACAAAGCATGTTCATCGCAAAACCCTACGCTCCATCAATCTCAATCCGACCGGTGTGGTCATTCAATCTCGAATCAGAGTTCAAACTCATCCGCTCTTTCGTTGATTCACATCCGATCATCTCAATGGACACCGAATTTCCCGGCGTTGTCGTCCGCCCTGACGGCATCACCGACTTATCATCTTACCATCGAACACCTGCCACTCATTACTCCGTCCTCAAGGCGAATGTCGACGGTCTTAATCTCATCCAAGTTGGTCTCACTTTATCAGATGCCGAGGGTAACCTTCCGAAATTAGAAAAGGAGTTTCTAATTTGGGAATTTAATTTCAGTGATTTTGATGTTGCACGTGATATTCACGCGCACGAGTCAATTGAATTGCTGAAGAGTCAGGGGATTGATTTCGAGAAGAATAAGGAATTTGGGATTGACTCATGGAAATTTGCTGAATTGATGATGAGTTCTGGACTTGTGTGTAATGAGGAAGTTAGCTGGGTGACATTTCATAGCGGATATGATTTCGGATATTTGGTGAAGGCGTTGACGCAGAGAGCTTTGCCTGATGAGCTTGCTGAGTTTCTGGTGCTGGTTAGGGTTTTCTTTGGGGAGAGTGTTTATGATGTGAAGCATTTGGTGAAGTTTTGTGAAGGGTTGTATGGTGGTTTGGATAGAGTTGGGAAGACGCTGAATGTGGATCGTGTTGCTGGGAAGAGTCATCAGGCTGGGTCTGATAGTTTGCTGACTCTTCATGCTTTTAGGAAGATTAAGGAAGTTCACTTTGGGAATGATGTTGATCGATTGATGAAGTATGCTGGTGTGTTGTATGGATTAGAAAATGTGGTTTAA

Protein sequence

>MS.gene034816.t1

MFTQSMFIAKPYAPSISIRPVWSFNLESEFKLIRSFVDSHPIISMDTEFPGVVVRPDGITDLSSYHRTPATHYSVLKANVDGLNLIQVGLTLSDAEGNLPKLEKEFLIWEFNFSDFDVARDIHAHESIELLKSQGIDFEKNKEFGIDSWKFAELMMSSGLVCNEEVSWVTFHSGYDFGYLVKALTQRALPDELAEFLVLVRVFFGESVYDVKHLVKFCEGLYGGLDRVGKTLNVDRVAGKSHQAGSDSLLTLHAFRKIKEVHFGNDVDRLMKYAGVLYGLENVV