AlfalfaGEDB | MS.gene034250

M. sativa cultivar XinJiangDaYe / MS.gene034250

KEGG: K12581
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04857.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034250.t1	XP_024638824.1	99.6	277	1	0	1	277	1	277	1.00E-160	575.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034250.t1	Q9SKZ2	79.9	269	54	0	9	277	7	275	3.0e-132	472.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034250.t1	A0A396HYE4	99.6	277	1	0	1	277	1	277	7.4e-161	575.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene034250	MS.gene065708	PPI
MS.gene034250	MS.gene78557	PPI
MS.gene034250	MS.gene048794	PPI
MS.gene034250	MS.gene53121	PPI
MS.gene034250	MS.gene41100	PPI
MS.gene034250	MS.gene034438	PPI
MS.gene034250	MS.gene85905	PPI
MS.gene034250	MS.gene065709	PPI
MS.gene034250	MS.gene68205	PPI
MS.gene034250	MS.gene025629	PPI
MS.gene034250	MS.gene53119	PPI
MS.gene034250	MS.gene065707	PPI
MS.gene034250	MS.gene000609	PPI
MS.gene034250	MS.gene55324	PPI
MS.gene034250	MS.gene020895	PPI
MS.gene034250	MS.gene034815	PPI
MS.gene034250	MS.gene85904	PPI
MS.gene034250	MS.gene030414	PPI
MS.gene034250	MS.gene84309	PPI
MS.gene034250	MS.gene034816	PPI
MS.gene034250	MS.gene68798	PPI
MS.gene034250	MS.gene72978	PPI
MS.gene034250	MS.gene41097	PPI
MS.gene034250	MS.gene030415	PPI
MS.gene034250	MS.gene85901	PPI
MS.gene034250	MS.gene38057	PPI
MS.gene034250	MS.gene72157	PPI
MS.gene034250	MS.gene21541	PPI
MS.gene034250	MS.gene81219	PPI
MS.gene034250	MS.gene35444	PPI
MS.gene034250	MS.gene38201	PPI
MS.gene034250	MS.gene053225	PPI
MS.gene034250	MS.gene38199	PPI
MS.gene034250	MS.gene53123	PPI
MS.gene034250	MS.gene78556	PPI
MS.gene034250	MS.gene85903	PPI
MS.gene034250	MS.gene048793	PPI
MS.gene034250	MS.gene070320	PPI
MS.gene034250	MS.gene014264	PPI
MS.gene034250	MS.gene35692	PPI
MS.gene034250	MS.gene35431	PPI
MS.gene034250	MS.gene53122	PPI
MS.gene034250	MS.gene53120	PPI
MS.gene034250	MS.gene007154	PPI
MS.gene034250	MS.gene72977	PPI
MS.gene034250	MS.gene41101	PPI
MS.gene034250	MS.gene72178	PPI
MS.gene034250	MS.gene72160	PPI
MS.gene034250	MS.gene025628	PPI
MS.gene034250	MS.gene72156	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034250.t1	AT2G32070	79.926	269	54	0	9	277	7	275	4.73e-169	469
MS.gene034250.t1	AT1G80780	79.323	266	54	1	9	274	7	271	2.46e-162	452
MS.gene034250.t1	AT1G80780	79.323	266	54	1	9	274	7	271	2.46e-162	452
MS.gene034250.t1	AT1G80780	79.167	264	54	1	9	272	7	269	1.87e-160	447
MS.gene034250.t1	AT5G10960	72.119	269	74	1	8	276	6	273	1.44e-146	412
MS.gene034250.t1	AT1G15920	63.433	268	91	2	7	268	9	275	1.78e-122	351
MS.gene034250.t1	AT1G15920	63.433	268	91	2	7	268	9	275	1.78e-122	351
MS.gene034250.t1	AT3G44260	53.571	280	119	8	1	270	1	279	1.25e-94	280
MS.gene034250.t1	AT5G22250	53.731	268	117	6	10	270	10	277	1.56e-94	280
MS.gene034250.t1	AT1G06450	37.405	262	147	6	15	272	10	258	1.56e-47	162
MS.gene034250.t1	AT1G61470	35.000	240	135	7	16	249	4	228	3.69e-34	125
MS.gene034250.t1	AT3G44240	34.934	229	136	5	27	253	2	219	4.69e-33	121
MS.gene034250.t1	AT1G27820	34.156	243	135	9	16	249	8	234	4.76e-31	117
MS.gene034250.t1	AT1G27890	34.568	243	134	9	16	249	3	229	1.59e-29	113

Find 45 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCATAGTGGATATGATTT+TGG	0.178563	4.1:+88667426	MS.gene034250:CDS
CGATTCAGATCCGTGAAGTT+TGG	0.267035	4.1:+88666968	MS.gene034250:CDS
TACTCACATCTTCTGCATTC+AGG	0.268390	4.1:+88667655	MS.gene034250:CDS
GAATTTCTTTAGTGGATCTT+TGG	0.269835	4.1:+88667689	MS.gene034250:CDS
TATGTTAAAGCTTATACAAT+TGG	0.337330	4.1:+88667152	MS.gene034250:CDS
AAGAACCCAACTTGTGTATC+AGG	0.337532	4.1:-88667483	None:intergenic
CAACTTGCGGCGAAGATGAT+AGG	0.351788	4.1:+88667208	MS.gene034250:CDS
TTGAAAGAGAATTTCTTTAG+TGG	0.358856	4.1:+88667681	MS.gene034250:CDS
GGTATCGATGCTAGGCGTTT+CGG	0.383381	4.1:+88667348	MS.gene034250:CDS
TGTTGGAGGTTAAGAGGGTT+GGG	0.387020	4.1:+88667604	MS.gene034250:CDS
CAGAGTTGTTGGAGGTTAAG+AGG	0.391647	4.1:+88667598	MS.gene034250:CDS
TCACAGCGAATTCTTCTTCA+AGG	0.398899	4.1:-88666999	None:intergenic
AAATATGCAGGGGTCTTGTA+TGG	0.400647	4.1:+88667714	MS.gene034250:CDS
TGATTATCCTTTCATTGCTA+TGG	0.408589	4.1:+88667038	MS.gene034250:CDS
TTGTTGGAGGTTAAGAGGGT+TGG	0.413971	4.1:+88667603	MS.gene034250:CDS
TCCTTTCATTGCTATGGATA+CGG	0.427277	4.1:+88667044	MS.gene034250:CDS
GGTATTGTTCTTCGACCTGT+TGG	0.437734	4.1:+88667075	MS.gene034250:CDS
ATGTTAAAGCTTATACAATT+GGG	0.439850	4.1:+88667153	MS.gene034250:CDS
GATTTGTCATCAAGCGGGTT+CGG	0.446291	4.1:+88667626	MS.gene034250:CDS
GAATAAGCTTGCAGAGTTGT+TGG	0.449673	4.1:+88667587	MS.gene034250:CDS
GCAGGGGTCTTGTATGGGTT+AGG	0.460666	4.1:+88667720	MS.gene034250:CDS
GTATTGTTCTTCGACCTGTT+GGG	0.461121	4.1:+88667076	MS.gene034250:CDS
AGAGTTGTTGGAGGTTAAGA+GGG	0.462475	4.1:+88667599	MS.gene034250:CDS
TAGAGAATTTAATGTTAATG+AGG	0.462708	4.1:+88667254	MS.gene034250:CDS
AATTTACCTGATACACAAGT+TGG	0.472874	4.1:+88667477	MS.gene034250:CDS
ATTTACCTGATACACAAGTT+GGG	0.507490	4.1:+88667478	MS.gene034250:CDS
GGATCTTTGGAGAAATATGC+AGG	0.509118	4.1:+88667702	MS.gene034250:CDS
CTATCATCTTCGCCGCAAGT+TGG	0.526576	4.1:-88667207	None:intergenic
GGTTATCGCTCCAAACTTCA+CGG	0.529023	4.1:-88666978	None:intergenic
TTTGTAATAGTCTTCATGGT+GGG	0.534821	4.1:+88667562	MS.gene034250:CDS
GTTTGATATCATACAGCATA+GGG	0.539432	4.1:-88667527	None:intergenic
TATGGGTTAGGTGTTGAGAA+TGG	0.543159	4.1:+88667732	MS.gene034250:CDS
ATGAACATGGTATCGATGCT+AGG	0.555439	4.1:+88667340	MS.gene034250:CDS
TAAGCTTGCAGAGTTGTTGG+AGG	0.562547	4.1:+88667590	MS.gene034250:CDS
TGTTTGATATCATACAGCAT+AGG	0.581668	4.1:-88667528	None:intergenic
ACAGGTCGAAGAACAATACC+AGG	0.581710	4.1:-88667072	None:intergenic
CATGGAAATCTTCCAACTTG+CGG	0.583679	4.1:+88667195	MS.gene034250:CDS
TCCGTATCCATAGCAATGAA+AGG	0.583831	4.1:-88667045	None:intergenic
GTTGGGATTTGTCATCAAGC+GGG	0.588282	4.1:+88667621	MS.gene034250:CDS
AATATGCAGGGGTCTTGTAT+GGG	0.597633	4.1:+88667715	MS.gene034250:CDS
GGTTGGGATTTGTCATCAAG+CGG	0.598328	4.1:+88667620	MS.gene034250:CDS
GCTATGGATACGGAATTCCC+TGG	0.614374	4.1:+88667054	MS.gene034250:CDS
GATCTTTGGAGAAATATGCA+GGG	0.629100	4.1:+88667703	MS.gene034250:CDS
ATCTTTGGAGAAATATGCAG+GGG	0.683756	4.1:+88667704	MS.gene034250:CDS
CAGGTCGAAGAACAATACCA+GGG	0.748306	4.1:-88667071	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGTTAAAGCTTATACAATT+GGG	+	chr4.1:88667153-88667172	MS.gene034250:CDS	20.0%
!!	TAGAGAATTTAATGTTAATG+AGG	+	chr4.1:88667254-88667273	MS.gene034250:CDS	20.0%
!!	TATGTTAAAGCTTATACAAT+TGG	+	chr4.1:88667152-88667171	MS.gene034250:CDS	20.0%
!!	TTCAAGAAAAATAATGAACA+TGG	+	chr4.1:88667327-88667346	MS.gene034250:CDS	20.0%
!!!	ACATATCAACATTATCTTTT+AGG	-	chr4.1:88667137-88667156	None:intergenic	20.0%
!!!	CATATCAACATTATCTTTTA+GGG	-	chr4.1:88667136-88667155	None:intergenic	20.0%
!!!	TTTTGAATGATAATGTTCAT+TGG	+	chr4.1:88667397-88667416	MS.gene034250:CDS	20.0%
!	TTGAAAGAGAATTTCTTTAG+TGG	+	chr4.1:88667681-88667700	MS.gene034250:CDS	25.0%
!!!	AAGTTTTGTAATAGTCTTCA+TGG	+	chr4.1:88667558-88667577	MS.gene034250:CDS	25.0%
!!!	TTTCATAGTGGATATGATTT+TGG	+	chr4.1:88667426-88667445	MS.gene034250:CDS	25.0%
	AATTTACCTGATACACAAGT+TGG	+	chr4.1:88667477-88667496	MS.gene034250:CDS	30.0%
	ATTTACCTGATACACAAGTT+GGG	+	chr4.1:88667478-88667497	MS.gene034250:CDS	30.0%
	GTTTGATATCATACAGCATA+GGG	-	chr4.1:88667530-88667549	None:intergenic	30.0%
	TGATTATCCTTTCATTGCTA+TGG	+	chr4.1:88667038-88667057	MS.gene034250:CDS	30.0%
	TGTTTGATATCATACAGCAT+AGG	-	chr4.1:88667531-88667550	None:intergenic	30.0%
	TTTGTAATAGTCTTCATGGT+GGG	+	chr4.1:88667562-88667581	MS.gene034250:CDS	30.0%
!	CATTGGATAACTTTTCATAG+TGG	+	chr4.1:88667414-88667433	MS.gene034250:CDS	30.0%
!	GAATTTCTTTAGTGGATCTT+TGG	+	chr4.1:88667689-88667708	MS.gene034250:CDS	30.0%
!	TTTTGTAATAGTCTTCATGG+TGG	+	chr4.1:88667561-88667580	MS.gene034250:CDS	30.0%
!!	AAGATGATAGGTTTTGTATC+TGG	+	chr4.1:88667220-88667239	MS.gene034250:CDS	30.0%
!!!	CAACATTATCTTTTAGGGTT+TGG	-	chr4.1:88667131-88667150	None:intergenic	30.0%
!!!	TCGTTTTGAGGTAAAATTAG+CGG	-	chr4.1:88666943-88666962	None:intergenic	30.0%
	TCCTTTCATTGCTATGGATA+CGG	+	chr4.1:88667044-88667063	MS.gene034250:CDS	35.0%
!	ATCTTTGGAGAAATATGCAG+GGG	+	chr4.1:88667704-88667723	MS.gene034250:CDS	35.0%
!	GATCTTTGGAGAAATATGCA+GGG	+	chr4.1:88667703-88667722	MS.gene034250:CDS	35.0%
!!!	ATTGAGCTTTTGAGACAAAG+TGG	+	chr4.1:88667297-88667316	MS.gene034250:CDS	35.0%
!!!	CTTACTTTTTCTGATGAGCA+TGG	+	chr4.1:88667177-88667196	MS.gene034250:CDS	35.0%
!!!	TTGCTTTTGAAATTCCCAAC+AGG	-	chr4.1:88667093-88667112	None:intergenic	35.0%
	AAGAACCCAACTTGTGTATC+AGG	-	chr4.1:88667486-88667505	None:intergenic	40.0%
	AGAGTTGTTGGAGGTTAAGA+GGG	+	chr4.1:88667599-88667618	MS.gene034250:CDS	40.0%
	CATGGAAATCTTCCAACTTG+CGG	+	chr4.1:88667195-88667214	MS.gene034250:CDS	40.0%
	GAATAAGCTTGCAGAGTTGT+TGG	+	chr4.1:88667587-88667606	MS.gene034250:CDS	40.0%
	GTATTGTTCTTCGACCTGTT+GGG	+	chr4.1:88667076-88667095	MS.gene034250:CDS	40.0%
	TACTCACATCTTCTGCATTC+AGG	+	chr4.1:88667655-88667674	MS.gene034250:CDS	40.0%
	TCACAGCGAATTCTTCTTCA+AGG	-	chr4.1:88667002-88667021	None:intergenic	40.0%
	TCCGTATCCATAGCAATGAA+AGG	-	chr4.1:88667048-88667067	None:intergenic	40.0%
!	AAATATGCAGGGGTCTTGTA+TGG	+	chr4.1:88667714-88667733	MS.gene034250:CDS	40.0%
!	AATATGCAGGGGTCTTGTAT+GGG	+	chr4.1:88667715-88667734	MS.gene034250:CDS	40.0%
!	GGATCTTTGGAGAAATATGC+AGG	+	chr4.1:88667702-88667721	MS.gene034250:CDS	40.0%
!	GTGAGCTTTTGATGTCATCT+GGG	+	chr4.1:88667370-88667389	MS.gene034250:CDS	40.0%
!!	ATGAACATGGTATCGATGCT+AGG	+	chr4.1:88667340-88667359	MS.gene034250:CDS	40.0%
!!	TATGGGTTAGGTGTTGAGAA+TGG	+	chr4.1:88667732-88667751	MS.gene034250:CDS	40.0%
!!	TGAATCGAATCGTCGTTTTG+AGG	-	chr4.1:88666955-88666974	None:intergenic	40.0%
	ACAGGTCGAAGAACAATACC+AGG	-	chr4.1:88667075-88667094	None:intergenic	45.0%
	CAGAGTTGTTGGAGGTTAAG+AGG	+	chr4.1:88667598-88667617	MS.gene034250:CDS	45.0%
	CAGGTCGAAGAACAATACCA+GGG	-	chr4.1:88667074-88667093	None:intergenic	45.0%
	CGATTCAGATCCGTGAAGTT+TGG	+	chr4.1:88666968-88666987	MS.gene034250:CDS	45.0%
	GGTATTGTTCTTCGACCTGT+TGG	+	chr4.1:88667075-88667094	MS.gene034250:CDS	45.0%
	GGTTATCGCTCCAAACTTCA+CGG	-	chr4.1:88666981-88667000	None:intergenic	45.0%
	GGTTGGGATTTGTCATCAAG+CGG	+	chr4.1:88667620-88667639	MS.gene034250:CDS	45.0%
	GTTGGGATTTGTCATCAAGC+GGG	+	chr4.1:88667621-88667640	MS.gene034250:CDS	45.0%
	TAAGCTTGCAGAGTTGTTGG+AGG	+	chr4.1:88667590-88667609	MS.gene034250:CDS	45.0%
	TGTTGGAGGTTAAGAGGGTT+GGG	+	chr4.1:88667604-88667623	MS.gene034250:CDS	45.0%
	TTGTTGGAGGTTAAGAGGGT+TGG	+	chr4.1:88667603-88667622	MS.gene034250:CDS	45.0%
!	GATTTGTCATCAAGCGGGTT+CGG	+	chr4.1:88667626-88667645	MS.gene034250:CDS	45.0%
!	GGTGAGCTTTTGATGTCATC+TGG	+	chr4.1:88667369-88667388	MS.gene034250:CDS	45.0%
	CAACTTGCGGCGAAGATGAT+AGG	+	chr4.1:88667208-88667227	MS.gene034250:CDS	50.0%
	CTATCATCTTCGCCGCAAGT+TGG	-	chr4.1:88667210-88667229	None:intergenic	50.0%
	GCTATGGATACGGAATTCCC+TGG	+	chr4.1:88667054-88667073	MS.gene034250:CDS	50.0%
!!	GGTATCGATGCTAGGCGTTT+CGG	+	chr4.1:88667348-88667367	MS.gene034250:CDS	50.0%
!	GCAGGGGTCTTGTATGGGTT+AGG	+	chr4.1:88667720-88667739	MS.gene034250:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	88666937	88667770	88666937	ID=MS.gene034250
chr4.1	mRNA	88666937	88667770	88666937	ID=MS.gene034250.t1;Parent=MS.gene034250
chr4.1	exon	88666937	88667770	88666937	ID=MS.gene034250.t1.exon1;Parent=MS.gene034250.t1
chr4.1	CDS	88666937	88667770	88666937	ID=cds.MS.gene034250.t1;Parent=MS.gene034250.t1

Gene Sequence

>MS.gene034250

ATGCCGCTAATTTTACCTCAAAACGACGATTCGATTCAGATCCGTGAAGTTTGGAGCGATAACCTTGAAGAAGAATTCGCTGTGATTCGAGAAATTGTTGATGATTATCCTTTCATTGCTATGGATACGGAATTCCCTGGTATTGTTCTTCGACCTGTTGGGAATTTCAAAAGCAATTATGATTATCATTACCAAACCCTAAAAGATAATGTTGATATGTTAAAGCTTATACAATTGGGTCTTACTTTTTCTGATGAGCATGGAAATCTTCCAACTTGCGGCGAAGATGATAGGTTTTGTATCTGGCAATTCAATTTTAGAGAATTTAATGTTAATGAGGATGTTTTTGCAAATGATTCAATTGAGCTTTTGAGACAAAGTGGAATTGATTTCAAGAAAAATAATGAACATGGTATCGATGCTAGGCGTTTCGGTGAGCTTTTGATGTCATCTGGGATTGTTTTGAATGATAATGTTCATTGGATAACTTTTCATAGTGGATATGATTTTGGTTATTTGTTGAAGTTGTTGACTTGTCAAAATTTACCTGATACACAAGTTGGGTTCTTTAATTTGATTAACATGTATTTCCCTATGCTGTATGATATCAAACATTTGATGAAGTTTTGTAATAGTCTTCATGGTGGGTTGAATAAGCTTGCAGAGTTGTTGGAGGTTAAGAGGGTTGGGATTTGTCATCAAGCGGGTTCGGATAGTTTACTCACATCTTCTGCATTCAGGAAATTGAAAGAGAATTTCTTTAGTGGATCTTTGGAGAAATATGCAGGGGTCTTGTATGGGTTAGGTGTTGAGAATGGACAGAGTACTCATTAA

Protein sequence

>MS.gene034250.t1

MPLILPQNDDSIQIREVWSDNLEEEFAVIREIVDDYPFIAMDTEFPGIVLRPVGNFKSNYDYHYQTLKDNVDMLKLIQLGLTFSDEHGNLPTCGEDDRFCIWQFNFREFNVNEDVFANDSIELLRQSGIDFKKNNEHGIDARRFGELLMSSGIVLNDNVHWITFHSGYDFGYLLKLLTCQNLPDTQVGFFNLINMYFPMLYDIKHLMKFCNSLHGGLNKLAELLEVKRVGICHQAGSDSLLTSSAFRKLKENFFSGSLEKYAGVLYGLGVENGQSTH